Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,547,471	2 bp→AT	coding (1028‑1029/2706 nt)	malT →	mal regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,547,471	0	T	A	97.6%	256.3 / NA	84	I343N (ATC→AAC)	malT	mal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (33/48);  total (35/49)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP009273	3,547,472	0	C	T	97.6%	233.4 / ‑5.5	84	I343I (ATC→ATT)	malT	mal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (31/49);  total (35/49)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAA  >  CP009273/3547335‑3547610                                                                                                                                          ||                                                                                                                                           cTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTgccgc                                                                                                                                 >  1:922646/1‑149 (MQ=255)   cGAAGAGATTGAGCGTCAGGGGTTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGccgccg                                                                                                                               <  2:207758/149‑1 (MQ=255)    gAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGGACTGGCGGCGGAGCTGCGGG‑AAAATACCCGTgccgcccc                                                                                                                              >  2:1007934/1‑147 (MQ=255)       gagaTTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGa                                                                                                                           >  1:666973/1‑149 (MQ=255)         gaTTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGaaa                                                                                                                         <  2:857267/149‑1 (MQ=255)           ttGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGc                                                                                                                       >  1:367691/1‑149 (MQ=255)             gtgCGTCAGGGGCTGTTTTTACAGCGGGTGGAGGTTACCGGCGAGTGGTTCTGCTATGCCCCGCTGTTTGGTAACTTCCTGCGGCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTg                                                                                                                     <  2:347144/147‑1 (MQ=255)              aGCGTCAGGGGCTGTTTTTACAGCGGCCGGATGAGAACGGCGAGTGGTTCTGCTATCACGCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGCACTGGCGGCGGAGCTGCCGG‑CAAAGCACCGTGCCGCCGCCGCAGGCTgg                                                                                                                    >  2:876532/1‑149 (MQ=255)              aGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTgg                                                                                                                    >  2:868397/1‑149 (MQ=255)                cGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGAt                                                                                                                  >  2:386882/1‑149 (MQ=255)                     ggggCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGccc                                                                                                             <  1:386882/149‑1 (MQ=255)                      gggCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCa                                                                                                            <  1:686727/149‑1 (MQ=255)                       ggCTGTTTTTACAGCGGATCGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACGTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGTAGCTTCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAg                                                                                                           <  2:511881/149‑1 (MQ=255)                        gCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCCCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCAAGTGGGAACTGGCGGCGAAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAACATGGATGGCCCAgg                                                                                                          >  1:491949/1‑149 (MQ=255)                        gCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCCGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAgg                                                                                                          <  1:374884/149‑1 (MQ=255)                         cTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCCGGATGGCCCAggg                                                                                                         >  2:821909/1‑149 (MQ=255)                          tGTTTTTGCTGCGGTTGGTTGATACCGGCGAGTGGTTTTGCTATTACTCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGa                                                                                                        <  1:1170901/149‑1 (MQ=255)                             ttttACAGCGGATGGATGGTACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGAttt                                                                                                     <  2:348282/149‑1 (MQ=255)                                tACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTccc                                                                                                  <  2:396409/149‑1 (MQ=255)                                 aCAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCa                                                                                                 <  2:697476/149‑1 (MQ=255)                                  cAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCCCTCGCTGTTTGGTAAGTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCATCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAg                                                                                                <  2:587574/149‑1 (MQ=255)                                  cAGCGGAGGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGACGCAGAAAGCTGGATGGCCCAGGGATTTCCCAg                                                                                                <  1:634860/149‑1 (MQ=255)                                   aGCGGATGGATGCTACAAGCAAGTGGTGATGCTATCACCCAATGTTAGGTAAAAACATGCACAAACGCTACGGATGGGGACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGCTTTcccggg                                                                                               >  2:566742/1‑146 (MQ=255)                                   aGCGGATGGATGATCGAGGCGAGTGGTTTTGCTATTTCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGc                                                                                               <  1:866198/149‑1 (MQ=255)                                   aGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGc                                                                                               <  2:603904/149‑1 (MQ=255)                                    gCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCg                                                                                              >  2:158640/1‑149 (MQ=255)                                     cGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGa                                                                                             <  2:738050/149‑1 (MQ=255)                                      tgatggatgTTACCGGCGTTTGGTTCTGCTATCACCCTCTGTTTGGTAACTTCCTGCGCCAGCTCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGTATTTCCCAGCGaa                                                                                            <  2:20464/148‑1 (MQ=255)                                      gggtggatgATACCGGCGTGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCGGCGCTGCCAGTGGGAACTGGCGGCGGAGCTCCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGaa                                                                                            <  2:611942/146‑1 (MQ=255)                                           gatgatAGCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGTCAGTGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAAt                                                                                       <  1:261591/149‑1 (MQ=255)                                              gatACCGGTCAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATtca                                                                                    <  1:1009496/149‑1 (MQ=255)                                                tACCGGCGAGTGGTTCTGCTATCACCCGCTTTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATtcatc                                                                                  <  2:1107307/149‑1 (MQ=255)                                                    ggCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATgc                                                                              <  1:815647/149‑1 (MQ=255)                                                         gTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGTCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTgg                                                                         >  1:67696/1‑149 (MQ=255)                                                         gTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCACGCGCTgg                                                                         >  2:913625/1‑149 (MQ=255)                                                          tGGTTCTACTATCACTCGCTGGTTGGTACGTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTggc                                                                        <  2:865173/149‑1 (MQ=255)                                                            gTTCTGCTATCACCCGCTGTTTGATAACTTCCTGCGCCAGCGCGGCCAGTGGGAACTGGCGGCGGAGCTGGCGGCACATCC‑CCGTGCCGCCGCCGAAAGCTAGATGGCCCAGNGATTTCCACACGAGCCAATTCATCAGTCGCTggcga                                                                      >  2:744085/1‑148 (MQ=255)                                                             ttCTGCTAGCACCCGCTGTATGGGAACTGCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTACCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTACCCAGCGAAGCAAGTCCACATGCGCTggcggc                                                                     >  2:693245/1‑149 (MQ=255)                                                              tCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCa                                                                    <  1:924881/149‑1 (MQ=255)                                                              tCTGCTATCACCCGCTGTATGGTAACTTCCTAGCCCAACCCTGCCAATCGCAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGCCGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCa                                                                    <  1:294485/149‑1 (MQ=255)                                                               cTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAg                                                                   <  1:1066280/149‑1 (MQ=255)                                                                tGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAgg                                                                  <  2:236207/149‑1 (MQ=255)                                                                   tATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGa                                                               >  1:335740/1‑149 (MQ=255)                                                                    aTCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAcgcggt                                                              >  2:1205688/1‑147 (MQ=255)                                                                    aTCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAAACACCGGGCCGCCGCACAAAGTTATACTTCTCTGTGATTTCCCAGTGAACCAATTCATCAGGCGCCGGCGGCTGCCGAt                                                              >  2:260163/1‑149 (MQ=255)                                                                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAATCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcg                                                           <  1:1210255/149‑1 (MQ=255)                                                                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcg                                                           <  2:1182204/149‑1 (MQ=255)                                                                        cccGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcgc                                                          >  1:178598/1‑149 (MQ=255)                                                                               tttGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgct                                                   >  2:17166/1‑149 (MQ=255)                                                                                ttGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgctg                                                  <  2:412008/149‑1 (MQ=255)                                                                                   gTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcgc                                               >  1:1133354/1‑149 (MQ=255)                                                                                   gTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcgc                                               <  1:516385/149‑1 (MQ=255)                                                                                    tAACTTCCTGCGTCAGCGCCGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAAGTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGcgcg                                              <  1:245412/149‑1 (MQ=255)                                                                                    tAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGAGGCGCTGATGCTGcgcg                                              >  2:891054/1‑149 (MQ=255)                                                                                     aaCTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCTCCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGa                                             <  1:44210/149‑1 (MQ=255)                                                                                      aCTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCAGGCAAATCC‑CCGTGCCGCCACAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGat                                            >  1:1035316/1‑149 (MQ=255)                                                                                         tCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATAtt                                         <  2:1024502/149‑1 (MQ=255)                                                                                          ccTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCATGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGGGATATTc                                        >  1:378622/1‑149 (MQ=255)                                                                                          ccTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGAAGGCGATGCGCTGATGCTGGGAGATCttg                                        >  2:304686/1‑148 (MQ=255)                                                                                          ccTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCCGCAGGCGATGCGCCGACGCTGCCCGatctac                                        >  2:304854/1‑145 (MQ=255)                                                                                          ccTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTAATCATGCACTGGCGGCAGGCGATGCGCTGATGCTGCGCGAGATTc                                        >  1:338731/1‑149 (MQ=255)                                                                                           cTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCt                                       <  2:826299/149‑1 (MQ=255)                                                                                            tGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTg                                      <  2:922646/149‑1 (MQ=255)                                                                                            tGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTg                                      >  1:706880/1‑149 (MQ=255)                                                                                              cgcCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCt                                    <  2:367691/149‑1 (MQ=255)                                                                                                ccAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTa                                  >  1:445327/1‑149 (MQ=255)                                                                                                 cAGCGCTGCCAGTGGGAACTTGCGGCCGAGCTGCCGG‑AAAATGACTGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATGTCCCAGCGAAGCAATTAATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCCTaa                                 <  1:509339/149‑1 (MQ=255)                                                                                                 cAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTaa                                 <  2:102387/149‑1 (MQ=255)                                                                                                 cAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTaa                                 >  1:1220311/1‑149 (MQ=255)                                                                                                       tGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGACGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGc                           <  1:487265/149‑1 (MQ=255)                                                                                                         ccAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCCGAAAGCTGGATGGCCAAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAAGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCt                         >  1:406814/1‑149 (MQ=255)                                                                                                         ccAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACCCCt                         >  2:493051/1‑149 (MQ=255)                                                                                                          cAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTg                        <  1:17166/149‑1 (MQ=255)                                                                                                            gTGGGAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGa                      <  1:261681/149‑1 (MQ=255)                                                                                                               ggAACTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCGGAAAGCTGGATGGCCCAGTGGGTTCCCAGCGCAGCAATTCATCATGCGCTGGCGACAGGCGAAACGCAGTTGCTGCAGGCTATTATGCTTAATAACGCCtagcgta                   >  2:562925/1‑143 (MQ=255)                                                                                                                   cTGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCCGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGtt               <  1:339916/149‑1 (MQ=255)                                                                                                                    tGGCGGCGGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGACAGGCGATGCGCTGATGCTGAGCGATATTATGCTTAATCACGCCTGGAGTCTGTTc              >  1:530003/1‑149 (MQ=255)                                                                                                                      gcggctGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCaa            <  1:1162592/149‑1 (MQ=255)                                                                                                                      gcggcgGAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCaa            <  2:1222707/149‑1 (MQ=255)                                                                                                                       cggcggAGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAAc           <  2:1035316/149‑1 (MQ=255)                                                                                                                           cgAGCTGGCGC‑AAAGTCCCCNTNCCTNCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCACCCGCTAGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATa       <  1:744085/148‑1 (MQ=255)                                                                                                                             aGCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGc     <  2:1220311/149‑1 (MQ=255)                                                                                                                              gCTGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCg    >  2:424412/1‑149 (MQ=255)                                                                                                                                tGCCGG‑AAAATCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGaa  <  1:868397/149‑1 (MQ=255)                                                                                                                                          ||                                                                                                                                           CTCGAAGAGATTGAGCGTCAGGGGCTGTTTTTACAGCGGATGGATGATACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGG‑AAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAA  >  CP009273/3547335‑3547610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑