| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,305,853 | C→T | 35.8% | D126N (GAC→AAC) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,305,853 | 0 | C | T | 35.8% | 45.7 / 45.0 | 57 | D126N (GAC→AAC) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (16/20); new base T (4/16); total (21/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.59e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01 | |||||||||||
CGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCC > CP009273/2305706‑2305999 | cGTTTTTCCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCg < 1:460992/149‑1 (MQ=255)cGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCg < 1:650833/149‑1 (MQ=255)cGTTTTGACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCg < 1:568436/149‑1 (MQ=255) tttttACCCTGGGACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGt < 2:315792/149‑1 (MQ=255) tACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTACAg > 2:936709/1‑149 (MQ=255) ccTGGTACTGAACAGCAAAGGTCAGGCCGTCAACCAGACCGAAGAAGTCAGCGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACCTCGGTCCACGaa < 2:607763/149‑1 (MQ=255) tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGCAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTAACCACCGAATTCTGGCAGTACGTCGTTCAAGGAAGt > 2:276250/1‑149 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 2:483496/149‑1 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:214808/149‑1 (MQ=255) aCTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACg < 1:781767/149‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCa < 1:534368/149‑1 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 2:250065/149‑1 (MQ=255) gCAAAGTTCAGGCCGTCAACCAGAACGAAGAAGTCAGTGTAACGTTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATGCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAAAaac > 2:519638/1‑149 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAaacaa < 1:752134/149‑1 (MQ=255) aggTTCAGGCCGGCAACCAGACCGAAGAAGTCAGTGTTACGGTCGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGGAGTTGTCAGAACCGTAGGTGTCACCTCCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaacaac < 2:205801/147‑1 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCAAAT‑GAATGAAGTTATAAAAACAGTCGGTGACACCACAGAAAACTGCCAGAACGAGAGTCCACGAACTACCGCCATAAACAACGc > 2:854513/1‑149 (MQ=255) ttCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGGGACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCACGAAGTTACGTCATAAACAACGcc < 1:653049/149‑1 (MQ=255) cAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 2:714227/149‑1 (MQ=255) gCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCCCGCCCCT‑GCCTGAAGTTGTCATAACCGTAGGTGTCACCACCGAATTCTGCCAGTAAGTCGGTCCCGGAAGTGACGTCATAAACAAGGCCGTAGt > 2:1123443/1‑149 (MQ=255) gTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGTTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 2:440332/149‑1 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGa < 1:145091/149‑1 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGa < 1:253034/149‑1 (MQ=255) acgccAGCCCGAAGAAGTCAGGGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGa < 2:538978/146‑1 (MQ=255) gaagaaGTCAGTGTTACGGACGGGCGCGAAGCCGTTACAAC‑CAGTTGGCATGACATTGTCAACAACAAATTAGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 2:875822/1‑149 (MQ=255) agaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaaa < 1:332519/149‑1 (MQ=255) agaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaaa < 1:955276/149‑1 (MQ=255) gaagGCAGTGTTACGGGACGTCGCGAAGCCCTTACCAGGCTGGC‑GCATGGAGGTGTCAGAACCGTAGGTGTCACCACCGAATTCGGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 2:116146/149‑1 (MQ=255) gaagCCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGACAAAGTAACCACCGAATTTTTTCATTACGTTGGTCCAGTAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:600394/149‑1 (MQ=255) ttACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAGCCCACATc > 2:862390/1‑149 (MQ=255) tagggcaGGTCGCGAAGCCGTAGCCACGTGGCT‑GCATGAATTGGTCAGAACCGTAGGTGTCACCACCGAATTCTGTCAGTACGCCGGTCCAGGAAGGTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGACCCcacgttc < 2:325119/143‑5 (MQ=255) gTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGCAttt > 2:1094388/1‑149 (MQ=255) cgcgAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCa < 1:845174/149‑1 (MQ=255) ttACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGaa > 1:151345/1‑149 (MQ=255) tACCACGCGGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGCATTTCAGACCTGCGAAt < 2:1226385/149‑1 (MQ=255) ccACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTCTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACTTCATAAACAACGGCGGAGTTACGACCGTAGTCGAAAGAACCCACACCCTGGAATTTCAGACCTGCGAATGc < 1:176158/149‑1 (MQ=255) ccACGCTGCG‑GCATGAAGTTGTCAGCACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAACCAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGc < 2:646944/149‑1 (MQ=255) aCGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACAGCCTGGAATTTCAGACCTGCGACTGCca > 2:1022222/1‑149 (MQ=255) gctgct‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca > 1:800703/1‑149 (MQ=255) gctgct‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca > 1:76633/1‑149 (MQ=255) cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGGCCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTcc > 2:229165/1‑149 (MQ=255) cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTcc < 1:399603/149‑1 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgtttt > 1:1198365/1‑149 (MQ=255) gAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttc > 1:224986/1‑149 (MQ=255) ccGTAGGGTTCACCACCGAATCCTGGCAGTCCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttcgtt < 2:278276/149‑1 (MQ=255) gTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAAAGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGATTGCCACACGGGTCTAGGAGTTgttttcgtttt > 1:812399/1‑149 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCCGGCGTTgttttcgttttc > 1:997737/1‑149 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGACgctttcgttttc > 2:1091252/1‑149 (MQ=255) gtgtCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAgc > 2:336904/1‑149 (MQ=255) tctCACCACCGAATTCTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAgcg < 1:441145/147‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGtt > 1:166928/1‑149 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTCCAGCGCTGTTGc > 2:529130/1‑149 (MQ=255) cTGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATc < 1:569695/149‑1 (MQ=255) tGGCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACCCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCt < 1:1029741/149‑1 (MQ=255) ggaGTACGTCGGTGCAGGAAGTTACGTCATAAACAAACGCGTAGCCACGACCGGAGACGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAAGGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGAGCTGTTGCCCTGGATCTGa < 2:566814/147‑1 (MQ=255) gcagcaCGTTGGGCCAGGAAGTTACGTCATAAACCACGCCGTAGTGACGACGCTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCGGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGa < 2:335928/149‑1 (MQ=255) gCAGTACGTTGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGa < 1:23014/149‑1 (MQ=255) gTCGGTCCAGGAAGTTAGGTCATAAACAACGCCGTAGTTATGACCGTAGTCGAAAGAACTAACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTccc < 1:613161/149‑1 (MQ=255) | CGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCT‑GCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCC > CP009273/2305706‑2305999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |