Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	776,421	T→C	6.9%	intergenic (+47/‑103)	lysW → / → valZ	tRNA‑Lys/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	776,421	0	T	C	6.9%	202.2 / 3.5	82	intergenic (+47/‑103)	lysW/valZ	tRNA‑Lys/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (21/51);  new base C (3/4);  total (25/57)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGG  >  CP009273/776273‑776569                                                                                                                                                      |                                                                                                                                                     gTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGGAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGAGGTTACGCGTGTAGGATAACGTTGCGt                                                                                                                                                      >  2:793629/1‑149 (MQ=255)      aTCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTT‑AAATCGACACGTCATGTTTAGCGTGTAGGTTGATGTTgtgttatta                                                                                                                                                 >  1:421910/1‑144 (MQ=11)      aTCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCa                                                                                                                                                 >  1:122222/1‑149 (MQ=255)       tCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCaa                                                                                                                                                >  2:452355/1‑149 (MQ=255)          cGTCATCACCCACCGCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTCAAGCGTGTAGGATAACGTTGCGTCAGCAACgg                                                                                                                                             <  2:435262/149‑1 (MQ=255)               tcaCCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGt                                                                                                                                        >  1:1236703/1‑149 (MQ=255)               tcaCCCACCACCGGGTCGTTAGCTCAGTTAGTAGAGCAGTTGACTTTTAATCAATAGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACACATGTTTAGGGTGTAGGATAAGGTTGCGACAGCGACGGGCCGt                                                                                                                                        >  1:874142/1‑149 (MQ=14)                 acccaccACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAg                                                                                                                                      <  2:873566/149‑1 (MQ=255)                  cccaccACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAgg                                                                                                                                     <  1:955281/149‑1 (MQ=255)                   ccaccaCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAggg                                                                                                                                    >  1:398467/1‑149 (MQ=255)                      ccaccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcga                                                                                                                                 >  2:81407/1‑149 (MQ=255)                       ccccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgag                                                                                                                                <  2:1184017/147‑1 (MQ=255)                       caccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgag                                                                                                                                <  1:66256/149‑1 (MQ=255)                       caccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgag                                                                                                                                <  1:45860/149‑1 (MQ=255)                       caccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgag                                                                                                                                <  1:1128828/149‑1 (MQ=255)                        accGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgagc                                                                                                                               <  1:1097115/149‑1 (MQ=255)                        accGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTCAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑CCATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgagc                                                                                                                               <  1:68682/149‑1 (MQ=255)                           gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaa                                                                                                                            <  1:762285/149‑1 (MQ=255)                            ggTCGTCAGCTGTGTTGGTAGGGGTGTTGACTTTTAAGCAATTGGTGGCTGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGGAGGCGGATGTTAGGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaag                                                                                                                           <  2:466078/149‑1 (MQ=14)                             gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagc                                                                                                                          >  1:675744/1‑149 (MQ=255)                               cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGagcgaagcga                                                                                                                        <  1:779896/149‑1 (MQ=255)                               cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                                                        <  2:1028263/149‑1 (MQ=255)                               cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                                                        <  1:1185642/149‑1 (MQ=255)                               cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTGTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                                                        <  1:793992/149‑1 (MQ=255)                                   aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGTCCCGTAGGGCGAGCGAAGCGAGTCa                                                                                                                    <  1:741771/149‑1 (MQ=255)                                   aGCCCAGTTGGTTGAGTAGTTGACTGTTACTCCGTTCGTCGCGGTTTCGAATCGTGGACGACCCATCAGTTTAA‑ACAACGAAGACAGGTGCTAAGCGTGTAGGATAACGTTGCTTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCa                                                                                                                    <  2:1190839/149‑1 (MQ=14)                                          tttgTAGACCAGTTTACTTTCAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGGCAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCa                                                                                                             <  2:477344/146‑1 (MQ=14)                                              tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTAGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCaagcc                                                                                                         >  2:406252/1‑145 (MQ=255)                                                  gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                                                                                                     <  2:899200/149‑1 (MQ=255)                                                    aGTTGACTTTTAATTAATTGGTCGCAGGTTCGAATCGTGCACTACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCCGCACGACccacca                                                                                                   <  1:874254/149‑1 (MQ=255)                                                    aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                                                   <  1:938482/149‑1 (MQ=255)                                                     tgtGACTTTTAATCATTTGGGCGCAGTTTCGAACCCTGCACGACCCCCCCCTCTTT‑ACACCGAACCCAGATGTAAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCcaccac                                                                                                  <  1:1159193/147‑1 (MQ=11)                                                      ttGACTTTTAATCAATTGGTCGCAGGTTCGGCACCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCGTCCTGCACGACCCACCACt                                                                                                 >  1:914262/1‑149 (MQ=255)                                                      ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACCGATGGTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGGCATCCTGCACGACCCACCACt                                                                                                 >  1:172291/1‑149 (MQ=255)                                                        gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCACCGGCCCGTCGGGACAGCGAAGCGAGTCATCCTGCTCGACCCACCACTaa                                                                                               <  1:913309/149‑1 (MQ=14)                                                         ccTTTTAACCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTCA‑ACAAAGTATTCAGTTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAAt                                                                                              <  1:288882/148‑1 (MQ=14)                                                         aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGCAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGCCGCGAGTCCTCCCGCCCGGCCCCCCCCTaac                                                                                              >  2:992663/1‑148 (MQ=14)                                                         aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAAt                                                                                              >  1:814766/1‑149 (MQ=255)                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGa                                                                                            >  2:59534/1‑149 (MQ=255)                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGATGCGTCAGCAACGGCCCGTAGGGCGTGCGAAGCTAGTCATCCTGCACGACCCACCACTAATGa                                                                                            <  1:804192/149‑1 (MQ=255)                                                                agcAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTGCA‑ACAGCGAAGGCGGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTg                                                                                       <  2:56798/147‑1 (MQ=17)                                                                    aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAAGGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGtt                                                                                   <  1:1160367/149‑1 (MQ=255)                                                                    aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGtt                                                                                   <  1:730479/149‑1 (MQ=255)                                                                          cctaGGTTCGCATCCNGCACGACCCACCAGGTTTA‑GCATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTgg                                                                             <  1:1140162/146‑1 (MQ=255)                                                                          cGCAGGTTCGAACCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGGGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGGTCGACTTCAGTCATCCTGCACGACCCACTACTAATGACGGTGGGTTCGGTgg                                                                             <  1:651609/149‑1 (MQ=17)                                                                             aGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCa                                                                                                                    <  1:478274/107‑1 (MQ=9)                                                                             aGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCa                                                                                                                    >  2:478274/1‑107 (MQ=16)                                                                                ttCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAagtag                                                                       <  2:175463/149‑1 (MQ=255)                                                                                   gAATCCTGCACGAGCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGttt                                                                    <  1:889468/149‑1 (MQ=255)                                                                                   gAATCCTGCACGACCCACCAGTTTTA‑ACATCGACGCCAGATGCTCGGCGGTCAGAAATACGTTGCGTCAGCAACGGCCCGTAGGGCGGGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGttt                                                                    <  1:35827/149‑1 (MQ=18)                                                                                      tCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGGGGAAGTAGTTTgta                                                                 >  1:1227778/1‑149 (MQ=255)                                                                                      ggctgtccGACCCACCAGCTTTA‑AGAGGGAAGAAAGATGTTACGCGTGGAGGATCACGGTGCGTCAGCAACGGCCCGTAGGGCGAGCGAGGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTgta                                                                 <  2:179772/142‑1 (MQ=21)                                                                                       ccTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATc                                                                                                                  >  1:330186/1‑99 (MQ=255)                                                                                       ccTGCACGACCCACCAGTTTTA‑ACATCGAACACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATc                                                                                                                  <  2:330186/99‑1 (MQ=12)                                                                                        cTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCCCTAATGACGGGGGGGTCGGTGGAAGGAGTTTggagt                                                               >  1:746625/1‑149 (MQ=18)                                                                                          tcACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTAt                                                             <  1:875582/148‑1 (MQ=255)                                                                                          gcccGACCCGCCAGTTTTA‑ACAGCGTAGACAGATGTTAAGCGTGTAGGATAACGTCGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTAt                                                             <  1:1083198/146‑1 (MQ=21)                                                                                          gcccGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTAt                                                             <  1:62535/146‑1 (MQ=255)                                                                                           cACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATc                                                            <  1:81407/149‑1 (MQ=255)                                                                                              gccccgcgaGTTTCT‑ACCTCGACAACAGATGTTACGCGTGTAGGATAACGGTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAg                                                         <  2:1027212/141‑1 (MQ=21)                                                                                              gACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTg                                                                   >  1:377378/1‑139 (MQ=255)                                                                                              gACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTg                                                                   <  2:377378/139‑1 (MQ=255)                                                                                                ccccccAGGTTTA‑ACATCGAATACAGATGTTAGGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGCCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAgcg                                                       <  2:1117791/149‑1 (MQ=21)                                                                                                cccACCAGTTTTA‑ATATCGAAGACAGATGTTAATCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGAGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGCTCGGTGGAAGTAGTTTGTAGTATCCAgcg                                                       <  1:755451/149‑1 (MQ=255)                                                                                                cccACCAGTTTTA‑AGATCGAAGGCAGGTGGTAAGGGGGTAGGATATCGTTGCGTGAGCAACGGCCCGTGGGGGGAGCGAAGCTAGAAATACAGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTCGTTTGTAGTATCCAgcg                                                       >  2:487863/1‑149 (MQ=255)                                                                                                ccaaccaGGTTCA‑ACATCCAAGACTGGAGTTAAGCGTGTAGGATAACCGTGCGTCAGCAACGGCCCGGAGGGCGAGCGAGGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAgcg                                                       <  1:336711/146‑1 (MQ=21)                                                                                                  caccaGTTTTA‑ACATCGAGGACAGATGTTAAGCGTGTAGGATAACGTTACGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGCAGTATCCAGCGCa                                                     <  2:754144/149‑1 (MQ=255)                                                                                                  caccaGTGTGA‑ACGGCGAAAGCCGATTGTAAGCGTGAAGGATAACGTTGCGGCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCa                                                     <  2:547798/149‑1 (MQ=18)                                                                                                  acacacttttA‑ACATCGAAGACAGACGTGAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCa                                                     <  2:496368/143‑1 (MQ=255)                                                                                                   caccgTTTTAGACAGCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCa                                                     <  2:95602/145‑1 (MQ=255)                                                                                                    ccaGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGGAGTAGTTTGTAGTATCCAGCGCAGt                                                   >  1:810665/1‑149 (MQ=255)                                                                                                    ccaGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGt                                                   >  2:1168410/1‑149 (MQ=255)                                                                                                               cATCCAAGACAGATGTTACGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGAt                                         <  1:337828/149‑1 (MQ=255)                                                                                                                   gAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGc                                     >  1:1194521/1‑149 (MQ=255)                                                                                                                      gACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCa                                  <  2:675744/149‑1 (MQ=255)                                                                                                                       aCAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAg                                 >  2:117011/1‑149 (MQ=255)                                                                                                                       aCAGATGTTAAGCGTGTACGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGGAGTATCCAGCGCAGTATCGGGTGATTAGCTCAg                                 <  1:793629/149‑1 (MQ=255)                                                                                                                        cAGATGTTAAGCGTGTAGGATAACCTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATCACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGc                                <  1:271608/149‑1 (MQ=255)                                                                                                                             gTTAAGCGTGTAGGATAACGTTGCGTCAGCAACCGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGCCCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTCGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGga                           <  2:317449/149‑1 (MQ=255)                                                                                                                                  gCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCa                      <  1:59534/149‑1 (MQ=255)                                                                                                                                     tgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCt                   >  2:904124/1‑149 (MQ=255)                                                                                                                                          gATAACGTTGCGTCAGCAACGGCACGTACGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCtt              >  1:684950/1‑149 (MQ=255)                                                                                                                                               cGTTGCCGCAGCAACGGCCCGTAGGGCGAGCGAAGCCAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAag         <  2:814766/149‑1 (MQ=255)                                                                                                                                                 ttGCGTCAGCAGCGGCCCGTGGGGCGAGCGAAGCGAGTCATCCTGCACCACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAagga       <  2:174685/149‑1 (MQ=255)                                                                                                                                                      tCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAggggg  >  1:598680/1‑149 (MQ=255)                                                                                                                                                      tCAGCAACGGCCCGTAGGGCGAGAGAAGCGAGTCATCCAGCACGACCCACCACTCATGACGGTGGGTTCGGTGGAAGAAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCGCCGCTGGGAGAGCACCTCCCTTACACGGAgggcg  >  1:667071/1‑147 (MQ=37)                                                                                                                                                      |                                                                                                                                                     GTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA‑ACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGG  >  CP009273/776273‑776569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑