| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,823,531 | A→G | 5.2% | V80A (GTG→GCG) | recA ← | DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,823,531 | 0 | A | G | 5.2% | 291.7 / 5.0 | 95 | V80A (GTG→GCG) | recA | DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor |
| Reads supporting (aligned to +/- strand): ref base A (47/43); new base G (2/3); total (49/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01 | |||||||||||
TTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACC > NC_000913/2823385‑2823679 | tttCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCAcc > 5:432538/1‑149 (MQ=255) tCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGGACAGCAGGTTGTCGATATAGACGCACAGTTTACGTGCGTAGATTGGGTCGAGCGCGTGTTCAGCTTCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTg > 1:278712/1‑149 (MQ=255) cAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGca < 7:333350/149‑1 (MQ=255) cTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGACGCTGCAGGTGATCGCC‑Gcagcgca < 7:67213/149‑3 (MQ=255) cTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGcagcgtca < 7:465129/149‑1 (MQ=255) cTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGcagcgtca > 6:206435/1‑149 (MQ=255) gCCGGTGTCCTGCTGGGAGCACAGCAGGTTGTCGTTATCGACGCGCAGTTTACGTGTGTAGATTGGGTCCACCCCGTTTTCAGGATCGATAAACCCACAGGGATTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTg < 4:254923/148‑1 (MQ=255) cGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATACACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGtt > 5:5619/1‑149 (MQ=255) tCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTAcc > 5:327391/1‑149 (MQ=255) tCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTAc < 8:470646/148‑1 (MQ=255) cGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGCAGGTGATCGCC‑Gcagcgcag < 6:320218/134‑4 (MQ=255) cGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGCAGGTGATCGCC‑Gcagcgcag > 5:320218/1‑131 (MQ=255) ggCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGa < 1:244912/149‑1 (MQ=255) gCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCCGCAGCGTCAGCGTGGTTTTACCGGaa < 6:188893/149‑1 (MQ=255) gAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTc > 3:1694/1‑149 (MQ=255) gCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCg < 3:104315/149‑1 (MQ=255) acaGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGt < 5:206435/149‑1 (MQ=255) agcagGTTGTCGATATCGACGCCCAGTTTATGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTcc > 4:53458/1‑149 (MQ=255) agcagGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTcc > 3:417598/1‑149 (MQ=255) agcagGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATCGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTcc > 6:369731/1‑149 (MQ=255) agGTTGGCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTa < 2:114181/149‑1 (MQ=255) gTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCg > 8:16927/1‑145 (MQ=255) gATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTcga < 6:432538/149‑1 (MQ=255) gACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGAt < 4:421675/138‑1 (MQ=255) gACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGAt > 3:421675/1‑138 (MQ=255) gCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGAtt < 8:444199/136‑1 (MQ=255) gCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGAtt > 7:444199/1‑136 (MQ=255) gCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGc < 8:241849/149‑1 (MQ=255) ccAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGcc > 6:209615/1‑148 (MQ=255) tgTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGACGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCa < 3:53458/147‑1 (MQ=255) cGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAg < 3:187935/149‑1 (MQ=255) cGTGCGTAGATTGGGTCCAGCGCGGGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGGCAg < 1:145934/149‑1 (MQ=255) gTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGc > 2:90890/1‑146 (MQ=255) gTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGc < 1:90890/146‑1 (MQ=255) cGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGacca < 3:290717/149‑1 (MQ=255) gTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGcagcgtca < 3:331640/86‑1 (MQ=255) gTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGcagcgtca > 4:331640/1‑86 (MQ=255) gTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGaccac < 6:327391/149‑1 (MQ=255) tAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATa < 5:263444/129‑1 (MQ=255) tAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATa > 6:263444/1‑129 (MQ=255) tAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGGCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGaccacc > 5:123322/1‑149 (MQ=255) gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTg > 1:101419/1‑149 (MQ=255) gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTg > 6:50016/1‑149 (MQ=255) ttGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGc < 4:1694/148‑1 (MQ=255) gTCCAGCGCGTGTTAAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGcccc > 3:361485/1‑147 (MQ=255) ccAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgc > 7:357823/1‑149 (MQ=255) cgcgTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGcgcg > 2:418965/1‑148 (MQ=255) cgcgTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGa < 4:417598/149‑1 (MQ=255) cgcgGGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCAG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGa < 2:278712/149‑1 (MQ=255) gcgTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGat < 1:418965/149‑1 (MQ=255) tgtTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATc < 7:16927/149‑1 (MQ=255) gCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGacc < 2:354573/122‑1 (MQ=255) gCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGacc > 1:354573/1‑122 (MQ=255) gCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTg > 7:472727/1‑148 (MQ=255) cATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGa > 2:368833/1‑148 (MQ=255) aTCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGCTTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGAAATCCACTGa > 2:393689/1‑147 (MQ=255) aTCGATAAACCCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATa > 2:317746/1‑103 (MQ=255) aTCGATAAACCCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATa < 1:317746/103‑1 (MQ=255) aTAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgcgc > 7:96867/1‑131 (MQ=255) aTAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgcgc < 8:96867/131‑1 (MQ=255) aaaCGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCg < 8:375417/77‑1 (MQ=255) aaaCGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCg > 7:375417/1‑77 (MQ=255) aaCGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAc < 5:180156/148‑1 (MQ=255) aCGCTCAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCTATCAGCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgc < 4:326878/125‑1 (MQ=255) aCGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACATGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGACCCAAgc > 3:326878/1‑125 (MQ=255) cGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAccag > 2:344142/1‑147 (MQ=255) gCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGt < 5:369731/149‑1 (MQ=255) cacaGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTa < 1:31800/149‑1 (MQ=255) acaGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCgg > 3:348807/1‑146 (MQ=255) acaGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCgg > 7:125900/1‑146 (MQ=255) caGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAg < 8:117315/137‑1 (MQ=255) caGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAg > 7:117315/1‑137 (MQ=255) aGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTagag < 5:50016/149‑1 (MQ=255) ttttACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGaaa < 5:260679/132‑1 (MQ=255) ttttACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGaaa > 6:260679/1‑132 (MQ=255) ccTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGGAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTc > 1:326112/1‑149 (MQ=255) cTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTgg > 8:437970/1‑42 (MQ=255) cTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTgg < 7:437970/42‑1 (MQ=255) cTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTc > 7:106565/1‑148 (MQ=255) ttCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCca > 2:145134/1‑149 (MQ=255) acgctgacgctgCAGGTGATCGCC‑GCAGCG‑CAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGAt < 7:411014/138‑1 (MQ=37) acgctgacgctgCAGGTGATCGCC‑GCAGCG‑CAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGAt > 8:411014/1‑138 (MQ=37) cgctg‑cgctgG‑GGCGATCACCTGCTGGGTGAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTTGAGATGGTTTCCACAt < 4:361485/148‑1 (MQ=255) cgctg‑cgctgC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATc < 1:368833/149‑1 (MQ=255) tg‑cgctgc‑gGCGATCACCTGCAGCGTCAGGGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCAt > 4:401094/1‑149 (MQ=255) tg‑cgctgc‑gGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCAt > 7:154816/1‑149 (MQ=255) tg‑cgctgc‑gGCGATCACCTGCAGCGTCAGCCTCGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCAGCCCCAAGCGCCATATCCAGTGAAAGCGCCCCGGTAGAGATGGTTTCCACATCCAt > 2:458380/1‑149 (MQ=255) gctgc‑gGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAc > 1:207146/1‑121 (MQ=255) gctgc‑gGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAc < 2:207146/121‑1 (MQ=255) ctgc‑gGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGACCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGaa > 5:167201/1‑149 (MQ=255) c‑ggcgATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACg < 6:123322/148‑1 (MQ=255) gcgATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCAGATGCATGGAACGGTc > 6:373452/1‑149 (MQ=255) gATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGCCGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCtt > 5:144150/1‑149 (MQ=255) aTCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCtt > 5:389872/1‑148 (MQ=255) aTCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTc < 4:348807/149‑1 (MQ=255) aCCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCAACGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGCAAGCGAACCGGTAGAGATGGTTTCCCCATCCAGGGAACGGGCTTCAcc > 2:411770/1‑149 (MQ=255) | TTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACC > NC_000913/2823385‑2823679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |