Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,968,458	C→T	Q182* (CAA→TAA)	wecA →	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,968,458	0	C	T	100.0%	184.2 / NA	61	Q182* (CAA→TAA)	wecA	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/28);  total (33/28)

TTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTAT  >  NC_000913/3968321‑3968597                                                                                                                                          |                                                                                                                                              ttGGTTACTTCCGGACGCTAGGGGCCGGCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAAcc                                                                                                                                          <  2:309115/143‑1 (MQ=255)     ttACTTCCTGACGCTATTTGCCGTATGGGCCACCTTGAGTGCGTTCAACATGTTTGATGGCATTGATGGCTTGCTGGGCGGGGTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGcc                                                                                                                                      >  1:955117/1‑143 (MQ=255)       actgtcTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTc                                                                                                                                    <  2:741649/138‑1 (MQ=255)         ttCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGTTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGAGGGTCGACGGGTAAACCAGCCTCGc                                                                                                                                  >  1:654036/1‑143 (MQ=255)         ttCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCGCGCGGTATGACTCTGTGGTTCGACGGGTCCACCAGCCTCGc                                                                                                                                  >  1:1068798/1‑143 (MQ=255)           ccTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGCTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCaa                                                                                                                                >  1:470975/1‑143 (MQ=255)              gACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTCAACCAGCCTCGCAATCt                                                                                                                             >  2:107086/1‑143 (MQ=255)                 gCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGt                                                                                                                          >  2:957289/1‑143 (MQ=255)                  ctttgtGCCGTCTGGGCGGCCATTAGTGCGTTGAACATGGTTGGTGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGTTTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTg                                                                                                                         <  1:115540/138‑1 (MQ=255)                    aTTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCt                                                                                                                       >  2:718781/1‑143 (MQ=255)                       tGCTGTCTGGGCGGTCATTAATGCGTTCATCATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGTGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTg                                                                                                                    <  1:424809/143‑1 (MQ=255)                        ctcGTCGGGGTGGCCATTAGTGCGTTCAACATCGTTGATGGCATTTATGGCTTGCTGCGCGCGTTGTCCTGCGTCGCGTTTGCAGCAATCGGGATGATTTTGTGGTTCGACGGGTAAACCTGCCTCGCAATCTGGTGCTTTGc                                                                                                                   <  1:874497/141‑1 (MQ=255)                          gctCTGGGCGGCCATTCATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATTGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCga                                                                                                                 <  1:881787/141‑1 (MQ=255)                          cctCTGCGCGGCCAGTAATGCGTGCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCACCTTTCCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCga                                                                                                                 <  1:880114/141‑1 (MQ=255)                           gTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCgat                                                                                                                <  1:594574/143‑1 (MQ=255)                           gTCTGGGCGGCCATTAATGCGTTCAACAGGGTTGATGGGATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCgat                                                                                                                <  2:675781/143‑1 (MQ=255)                               gtgtggcCATTAGTGCGTTCGACATGGTGGATGGCATTGATGGCTTGCTGGGCGGGTTGTTCTGCGTCTCGTTTGCAGCAAGCGGTATGATGTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATc                                                                                                            <  1:312374/139‑1 (MQ=255)                                ggcggcCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATcg                                                                                                           >  1:73816/1‑143 (MQ=255)                                 gcggcCATTGATGCGTTCAACATGGTTGATGTGATTGATGGGTTGCTGCGGGGCTTGCCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATcgc                                                                                                          <  2:102183/143‑1 (MQ=255)                                 gcggcCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGGATGATTTTGTGGTTCGACGGGTAAACCAGCCCCGCAATCTGGGGCTTTGCggaaaacgt                                                                                                          >  2:1141661/1‑135 (MQ=255)                                         tagggCGTTCAACACGGTTGATGGCATTGATGTCTGGCTGTGCGGTTTGTCCTGCGTCTCTTTTGCAGCTATCTTTTTTTTTTTGTTTTTTTACGGTTAAACCTGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATcc                                                                                                  <  2:873549/139‑1 (MQ=255)                                               gTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCAt                                                                                            >  1:738524/1‑143 (MQ=255)                                               gTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAGTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCAt                                                                                            >  2:401960/1‑143 (MQ=255)                                                ttCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCATGCGTCTCGTTTGCAGCAATCGGTGTGATGTTGTGGTTCGACGGGTAAACCAACCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCCAACTGCCATa                                                                                           >  1:340776/1‑143 (MQ=255)                                                    aCATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAc‑atc                                                                                      <  2:738524/143‑1 (MQ=255)                                                              tGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCAATCCTGCCATAC‑CTCATGCTTAAcc                                                                            >  1:537311/1‑143 (MQ=255)                                                              tGGCATTGATGGCTTGCTGGGCGGGATGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAGTTTGTGGTTCGACGGGTAAACCAGCATCGCAAACTGGTGCTTAGCGAGGATCGCCGCCATCCTGCCACAC‑ATCATGATCAAcg                                                                            >  1:94184/1‑142 (MQ=255)                                                                 ctttGATGGCTTTATCCTCGCGTTGTCCTGCGTGTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGCCGGGTAAACCAGTCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTg                                                                         <  1:127884/141‑1 (MQ=255)                                                                 cgttgttgGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCGATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTg                                                                         <  1:824286/141‑1 (MQ=255)                                                                 cATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTg                                                                         <  1:789600/143‑1 (MQ=255)                                                                       tGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCACTCTGCTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATcc                                                                  >  2:435052/1‑143 (MQ=255)                                                                          cgtGCGGGGCGGGGTGTCCTGCGTGTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTgg                                                               <  1:540600/141‑1 (MQ=255)                                                                          cTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCTGCAATCGGTATGATTTTGTGGTTCGACGTGTAAACCAGCCTCGCAATCTGGGGCTGGGCGATGAGCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTgg                                                               <  2:60995/143‑1 (MQ=255)                                                                           ttGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCACTCTGGTGCTTTGCGATGCTAGCGCCTGCCCGGCTATAC‑ATCATGCTTAACCTTG‑GTGTcgtcgg                                                              >  1:241691/1‑138 (MQ=255)                                                                           ttGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGTTGCTCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTggg                                                              <  2:601937/143‑1 (MQ=255)                                                                           ttGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACGTTG‑GTGTCGTggg                                                              >  2:178814/1‑143 (MQ=255)                                                                               tGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTCAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTcgc                                                          <  1:672412/143‑1 (MQ=255)                                                                                          tCCTGCGTCTCGTTTGCAGCAAGCGGTATGGTTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATACTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCGGGGTCGCCGCTACACAGt                                               >  1:418390/1‑143 (MQ=255)                                                                                           ccTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTc                                              <  1:718781/143‑1 (MQ=255)                                                                                            cTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTCTCCTGGGTCGCCGCTCCCACGTCt                                             >  1:780342/1‑143 (MQ=255)                                                                                            cTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACACAGTCt                                             >  1:773821/1‑143 (MQ=255)                                                                                            cTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCt                                             >  2:1035189/1‑143 (MQ=255)                                                                                            cTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCt                                             <  2:497278/143‑1 (MQ=255)                                                                                               cGTCTCGTTCGCAGCAATCCGTATGATCTTCTGGTTCGACGGGTCAACCAGCCTCGCAAGCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTa                                          <  1:583207/143‑1 (MQ=255)                                                                                                 tctcGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGAGCAATGAGATGATCGCCGCCATCCTGCCATCC‑ATCATGCTTAACCTTG‑GTATCGTGGGTCGCCGCTACAAAGTCGTTATg                                        >  2:1041604/1‑143 (MQ=255)                                                                                                  ctcGTCTGCAGCACTCGGTCTGAGTTTGTGGTTCGACGCGTAAACCAGCCTCGCAATCTGGTTCTGTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATgg                                       <  1:652654/143‑1 (MQ=255)                                                                                                   tcGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATggg                                      >  2:544128/1‑143 (MQ=255)                                                                                                   tcGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCATCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCGTTCTggg                                      >  1:272878/1‑143 (MQ=255)                                                                                                           agcaATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTGATGGGTGATGCgg                              >  2:749155/1‑143 (MQ=255)                                                                                                            gcaATCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACACAGTCTTTATGGGTGAGTCggg                             >  1:361703/1‑143 (MQ=255)                                                                                                              aaTCGGTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCa                           >  2:298726/1‑143 (MQ=255)                                                                                                                  ggTATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGttc                       <  2:857028/143‑3 (MQ=255)                                                                                                                    tATGATTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGc                     >  1:311701/1‑143 (MQ=255)                                                                                                                        aTTTTGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTggt                 >  2:899680/1‑141 (MQ=255)                                                                                                                         ttttGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGAtt                <  2:73816/143‑1 (MQ=255)                                                                                                                         ttttGTGGTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTCTATCGGTGATGCGGGCAGTACGCTGAtt                >  2:1121568/1‑143 (MQ=255)                                                                                                                           tggtggtgCGACGGGTAAACCAGCCTCGCAATCTGGGGCGTTGGGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTgg              <  2:1074641/141‑1 (MQ=255)                                                                                                                             gtgGTCCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCATTGATCGCCGCCATACTG‑TACACGGTTATCCTGAACCAAGCAAAGCCTGGG‑CTTCTGTACAAAGTCTTTATTGGTGATGCGGGCAGTACGCTGATTGGtt            <  2:270169/143‑1 (MQ=255)                                                                                                                               ggTTCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCAGGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGtttt          >  1:766168/1‑143 (MQ=255)                                                                                                                                 ttCGACGGGTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCACCCTGCCATAC‑ATCATGCTTCACCTTG‑CTCTCCTGGGTCGCCGCTACAAAGTCCTCACGGGTGATGCGGGCAGTACGCTGATCGGGTTTAc        >  1:785410/1‑143 (MQ=255)                                                                                                                                       gggTAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGatat  >  1:829393/1‑143 (MQ=255)                                                                                                                                          |                                                                                                                                              TTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATAC‑ATCATGCTTAACCTTG‑GTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTAT  >  NC_000913/3968321‑3968597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑