BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,289,076	C→A	15.5%	Q89K (CAG→AAG)	yraJ →	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,289,076	0	C	A	15.5%	385.8 / 16.5	163	Q89K (CAG→AAG)	yraJ	putative outer membrane protein
Reads supporting (aligned to +/- strand): ref base C (102/32); new base A (3/24); total (105/58)
Fisher's exact test for biased strand distribution p-value = 2.42e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

CGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTGCACTGTACCGTGA  >  NC_000913/3288842‑3289308

cgccccccGAAACATGTGGCGCTCATCCGCAAACGGTGTTTGCCGATGGTGCTTACTGGCAGCGGCATGCTTTGCATTACCGCGAAATCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTTG‑TATGCAAACAACCGATCTGTCGCT‑TTTTGCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGaa                                                                                                                                                                                                                                       <  1:860664/233‑1 (MQ=255)

cGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGaa                                                                                                                                                                                                                                       <  2:111053/239‑1 (MQ=255)

 gCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGaaa                                                                                                                                                                                                                                      >  2:195353/1‑239 (MQ=255)

  cacaCCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTACCGCCGAAAAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGAGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGAAATCT‑GGCTGAAT‑AAAAAAAAGGTTTC‑ACAGaaaa                                                                                                                                                                                                                                     >  2:699866/1‑239 (MQ=255)

       cGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACACCT‑ACCAG‑GAACTTATCA‑GGTTGATATAT‑GGCTGAAT‑AAAAAGAAGGTTTC‑AAAAAAAAAAAtt                                                                                                                                                                                                                                >  1:870409/1‑239 (MQ=255)

        gAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTa                                                                                                                                                                                                                               <  1:908978/239‑1 (MQ=255)

               ttGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑Ta                                                                                                                                                                                                                        >  2:1107462/1‑238 (MQ=255)

                 ggCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAACAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATACCT‑GACTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TAcc                                                                                                                                                                                                                      >  2:1024093/1‑238 (MQ=255)

                 ggCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TAcc                                                                                                                                                                                                                      >  2:682798/1‑238 (MQ=255)

                                  gttgttTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGGAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GACCTTATCA‑GGTTGATATCT‑GGCGGATT‑AAAAAGAAGGTTCA‑ACAGAAACAAATTACATT‑TACCGCCAATGCAAAACaaat                                                                                                                                                                                                     >  1:624107/1‑236 (MQ=255)

                                    tgttTGCCGATGGTGCTCACTGGCAGCGTCATGCTTTGCACTACAGCTCACGCCGAAGAGTCTTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGT‑TATGCAAACAACCGCTCTGTAGCG‑TTTTTCAAAAAAAAACGCACACCT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑TCCTGAAT‑AAAAAGAAGATTGC‑CCAGAAAAAAATACCATT‑TACGGCCaaacaagaacaaccttg                                                                                                                                                                                                  >  1:988810/1‑224 (MQ=255)

                                     ttttGCCGATGGTGCTCACTGGCAGCGGCATGCTTGGCACTACCGCAAACGCCAAAGAGTTTTATTCCGACCCCATTATGCGGGAAACCAAAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATCAACAACT‑CCCAG‑GACCTTATAA‑GGTTGATATCT‑GGTTGAAT‑AAAAAAAAGTTTTC‑CCAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTg                                                                                                                                                                                                 <  2:1000071/238‑1 (MQ=255)

                                           cggtgggtctcccgggCAGCGGCAGTCTTTGCCCGTCCGTAAACGCCGAAGAGTATTATTTCGACCCCATTAGGCTGGAAACCACAAAAAGTGG‑TATGAAAACAACCAATCTGTCGCT‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑CAAGAAAAAAATTCCATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCca                                                                                                                                                                                           <  2:1202889/225‑1 (MQ=255)

                                              tGGTGCTCACTGGCAGCGGCATGCTTTGCAATACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTCTGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGCTCTGTCGCG‑GTTTTCAAAAAAATTTGCACAACC‑ACCAT‑GAACTTATCA‑GGTTGTTATCT‑TGCTGAAT‑AAAGAGCAGGCTTC‑AAAGAAAAAAATAACATT‑TACCCCCAATGAATGGCAACTTTTgacaccacaa                                                                                                                                                                                        >  1:720432/1‑230 (MQ=255)

                                                       cTGGAAGCGGAATGTTTTTCACTACCGCTAACGCGGAGGAGTATTATTTCGACCCCATTATGCTGGAAACCAAAAAAAGTGG‑TATGCAAACAACCGATCTGGCGCT‑TTTTTAAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTAAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTa                                                                                                                                                                               <  2:526593/239‑1 (MQ=255)

                                                           caccgtcATGCGTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GCATGAAT‑AAAAAGAAGGTTTC‑ACGGAAAAAAATTACATT‑TAACCCCAAGGAAAAACACATCCTGCAGCCAAAGTTAACGGAAAaac                                                                                                                                                                           >  1:315655/7‑239 (MQ=255)

                                                               ggCTTGTTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTAGGCTGAAAACCAAAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCT‑TTTTTAAAAAAAATAGGCACAACT‑CCCAG‑GAATTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑AAAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGaacaa                                                                                                                                                                         <  2:437542/237‑1 (MQ=255)

                                                                gCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAATTTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCCCAGTTTCCGGTAGAACAACt                                                                                                                                                                       >  2:785628/1‑238 (MQ=255)

                                                                 ctttttttGCACTCCCGATAAGCCCAAAGATTTTTTTTTCGCCCCAATTAGGGTGGAAACCAAAAAAAGTGG‑TATGCAAACAACCGACTTGTCGCT‑TTTTTCAAAAAAATATGCACAACA‑ACCAG‑GATTTTATCA‑GTTTGATATCT‑GGTTGAAT‑AAAAAGAAGTTTTC‑ACAGAAAAAAATTAATTT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTa                                                                                                                                                                      <  2:487671/233‑1 (MQ=255)

                                                                    gCTTTGCACTACCGCTAACGCCGCAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCCCAGTTTACGGTAGAACAACTACGTg                                                                                                                                                                  >  2:855133/1‑239 (MQ=255)

                                                                        tcccctcccgctaaccggaaaGGGTATTATTTCAACCCATTTATGCTGGAAACCAAAAAAAGGGG‑TATGAAACCAACCGATCTGTCGCG‑TTTTTAAAAAAAATAGCCAAAACT‑CCCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAAAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt                                                                                                                                                              <  2:517081/220‑1 (MQ=255)

                                                                        tGCCCTCCCGCTAACCCCGAAGAGTTATATGTCGACCCCTTTATGCTGGAAACCAAAAAAAGTGG‑TATGCAAACAACCGATCTGCCGCT‑TTTTCAAAAAAAAGATGCAAAATG‑ACCAG‑GAACTTAACG‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAGTGTTTC‑CCAGAAAAAAATTCCATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt                                                                                                                                                              <  2:306006/239‑1 (MQ=255)

                                                                        cccccacccccaaccaccaaaGATTATTTTTCCGCCCCAATAATGTTGAAAACCAAAAAAAGTGG‑TATGAAAACACCCTATCTGCCTGT‑TTTTAAAAAAAAATATGAAAATAT‑CCCAG‑GAACTTATAA‑GGTTGATATCT‑GGCGGAAA‑AAAAAGAAGGTTTA‑AAAGAAAAAAATTACATT‑GACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt                                                                                                                                                              <  1:1113089/220‑1 (MQ=255)

                                                                               ccGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTAtt                                                                                                                                                       >  2:401136/1‑239 (MQ=255)

                                                                               ccGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGACCTGGGAAtt                                                                                                                                                       >  2:1257603/1‑239 (MQ=255)

                                                                               ccGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTAAATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCAACAGTTTACGGAAGAAAAACAAAGGGAGCGGGGAAtt                                                                                                                                                       >  1:899864/1‑239 (MQ=255)

                                                                               ccGATAACGCCAAAGAGTATTTTTGCGACCCAATTTGGCTGGAAACCAAAAAAAGTGG‑AAGGCAAACAACCGATCTGCCGGG‑TTTTTAAAAAAAATAGGAAAAACT‑ACCAG‑GAACTTATCA‑GTTTGATATCT‑GGTTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTAtt                                                                                                                                                       <  2:405662/239‑1 (MQ=255)

                                                                                  cTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAg                                                                                                                                                    >  2:1146885/1‑239 (MQ=255)

                                                                                  cTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAg                                                                                                                                                    >  2:557758/1‑239 (MQ=255)

                                                                                           aaGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGaa                                                                                                                                           >  2:867130/1‑239 (MQ=255)

                                                                                                   tattTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAAAAGGTTTC‑ACAGAAAAAAATTAAATT‑TACCGCCAATGCAGAGCAACTTCTACAGCCAAAGTTTACGGTAGAAAAACAACTTGAGCTGGGAATTAAGGTGGATGAAATCCCGgc                                                                                                                                   >  1:339203/1‑239 (MQ=255)

                                                                                                    tttccccccccttttgtcTGAAACCCAAAAAAAGGGT‑TAGAAAAACAACCGTTTTTGCGCG‑TTTTAAAAAAAAATATACAAAACT‑ACCAG‑GAAATTAAAA‑GGTTGATATCT‑GGCTGAAA‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGgcg                                                                                                                                  <  1:140874/222‑1 (MQ=255)

                                                                                                        cGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGCAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCAACAGTTTACGGTAGACCAACTACGTAAGCTGGGTATTAAggctgtctcttttaaacatctcc                                                                                                                              >  2:1326289/1‑218 (MQ=255)

                                                                                                          acatccttAGGCTGTCAACCACAAAAAGTGG‑TATGCAAACACCCGATCTGTCGCG‑TTTTTAACAAAAATATGCACAATT‑AACAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGATT‑AAAAAGAAGACTTA‑ACAAGAAAAACTTAACTT‑CACCGCCAATGCAAAGCAACTTCTCCAGCAACAATTTAAGGATAACCAACCACGTCGGCTCGGGTTTAAAGCGGACGAAATccccccgaagccc                                                                                                                            >  1:417953/7‑229 (MQ=255)

                                                                                                          aCCCTATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCcctggct                                                                                                                            >  2:1282493/1‑239 (MQ=255)

                                                                                                          aCCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAACAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGAAACTTCTGCAGCAACAATTTACGGTAGAAAAACAACATGAGCTGGGTTTTAGGGGGGATAAAATCCGGgcgcgcttg                                                                                                                            >  1:1257114/1‑234 (MQ=255)

                                                                                                          aCCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATCTCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAACAATTACATT‑TACCGCCAATGCAGAGAAACTTCTGCAGCCACCATTTACGGTAGAACAAATAGGTGAGATGGGTCTTCAGGTGTGTGAAAACCCTGCgctg                                                                                                                               >  2:937952/1‑236 (MQ=255)

                                                                                                          aCCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCcctg                                                                                                                               >  2:96183/1‑236 (MQ=255)

                                                                                                          aCCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGGATTAAGTTGGATGAAATCCCGCCgctggcg                                                                                                                            >  2:798538/1‑238 (MQ=255)

                                                                                                          aCCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCgctggct                                                                                                                            >  2:212120/1‑239 (MQ=255)

                                                                                                          aCCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCAACAGTTTACGGTAAAACAACTACGTTAGCGGGGTATTAAGGGGGATGAAATACCGGAgctggcg                                                                                                                            >  1:683548/1‑238 (MQ=255)

                                                                                                          aCCCCATTATGCTGGAAACAACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCgctg                                                                                                                               >  2:921864/1‑236 (MQ=255)

                                                                                                              cATTTTGCGGAAAACAAAAAAAAGGGG‑TACGAAAAAACCAGTACTGTGGGG‑TTTGTAAAAAAATAAAGCAAATGT‑ACCAG‑AAATTTAGAA‑GGTT‑AAATCTGGGCGAAAA‑AAAAAGAAGTTTCC‑AAAGAAAAAATTTACATT‑AACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGaaa                                                                                                                        <  1:817456/239‑1 (MQ=255)

                                                                                                              cATTAGGCTGAAAACCAAAAAAAGTGT‑TATGCAAACAACCGATCTGGCGCT‑TTTTCAAAAAAAATAGGCCCAACT‑ACCAG‑GAACTTATCA‑GGTGGATATCT‑GGCAGAAA‑AAAAAGAAGGTTGC‑AAAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGaaa                                                                                                                        <  2:26379/239‑1 (MQ=255)

                                                                                                               aTTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGaaaa                                                                                                                       >  2:80549/1‑239 (MQ=255)

                                                                                                                  ttttttaaaaCCA‑AAAAAGTGGTTATGAAAACACCTGTTTTGTCGCG‑TTTTTAAAAAAAATACGAACATCT‑‑CCAGACACCATATCA‑GTGGTATCTCT‑GGGTGAAA‑AAAAGAAATGTTTA‑ACAAAAAAAATTTACTTT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGATTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGa                                                                                                                    <  1:212120/232‑1 (MQ=255)

                                                                                                                     cTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGCCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑CCAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTATACCAACTACGTCGCACGGGCATTAAGGTGGATGAAACCCCGACGCTGGCTAAAAAAGATGa                                                                                                                 >  1:26379/1‑239 (MQ=255)

                                                                                                                        gAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGaaa                                                                                                                        >  2:62582/1‑229 (MQ=255)

                                                                                                                        gAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAAACCCGGGGCTGGCTGAAAAAGATGAGGa                                                                                                              >  1:1178372/1‑239 (MQ=255)

                                                                                                                        gAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGa                                                                                                              >  2:912760/1‑239 (MQ=255)

                                                                                                                        gAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGa                                                                                                              >  2:369531/1‑239 (MQ=255)

                                                                                                                         aaaCCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAAGGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAAATACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGagaccgaa                                                                                                             >  2:436747/1‑232 (MQ=255)

                                                                                                                             cacaAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAACAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACACCTACGTGAGCTGGGTTTTAAGGTGGATGAAATCCCGACCCGGGCTGAAAAAAAAGACGATAACg                                                                                                         >  1:157114/1‑239 (MQ=255)

                                                                                                                               aaaaaaGTGG‑TAAGCAAGAACCCGATCTGCCCTG‑TTTTACAAAAAAATATGAACAACT‑ACCAG‑GAATTTATGA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTAAATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTg                                                                                                       <  2:988810/238‑1 (MQ=255)

                                                                                                                                    atggg‑TATGAAAAAAACCGATCTGTCGCG‑TTTTCAAAAAAAATATGCACAACC‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGTTAAAA‑AAAAAAAAGGTTTC‑ACAAAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCaa                                                                                                  <  2:624107/236‑1 (MQ=255)

                                                                                                                                    agggg‑TAAGAAAACACCCGATCTGTCGCT‑TTTTTAAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCG‑GGCTGAAA‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGAAGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCaa                                                                                                  <  2:339203/236‑1 (MQ=255)

                                                                                                                                     aggggaaTGCAAACACCCGTCCTG‑CTCGTTTTTTAAAAAAAATATGCACAACT‑ACCAG‑GAACTTACCA‑GGTTGAAATCT‑GGCTAAAT‑AAAAAGAAGGTTCC‑AAAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCaa                                                                                                  <  2:29959/233‑1 (MQ=255)

                                                                                                                                      tgtgtaatgaaacaaacGATTTGTCGGC‑GTTTTAAAAAAAATAAACACAAATCACCGG‑GAA‑TTAACAGGGTTAAAATCT‑GGCTAAAT‑AAAAAAAAGGTTTC‑AAAAAAAAAATTTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCaa                                                                                                  <  2:655608/230‑1 (MQ=255)

                                                                                                                                        g‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACtg                                                                                               >  2:838672/1‑237 (MQ=255)

                                                                                                                                        g‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGAATGAAATCCCGGGGCTGGCTGAAAAAAATAAcaaaaagggaacaaaacgc                                                                                              >  1:28127/1‑221 (MQ=255)

                                                                                                                                               aaacacgcgatcgtgcgtg‑TTTTTAAAAAAAATATGCACAACT‑ACCAG‑GAACTTAGCA‑GGTTGCTATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGaa                                                                                        <  2:1113507/221‑1 (MQ=255)

                                                                                                                                               aaaCAACGGATCTTCGGTG‑TTGTTAAAAAAAAAATGCACAACT‑ACAAG‑AAACTTATAA‑GGTTAATATTG‑GGCTAAAT‑AAAAAGAAGGTTTC‑AAAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGaa                                                                                        <  2:1299989/239‑1 (MQ=255)

                                                                                                                                                 acaacCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACACATACATGAGCTGGGGATTAAGGGGGATGAAATCCCGGCCCTGGCTGAAAAAAATGACGAAAGCGGGATCAACACGCTTGAACa                                                                                      >  1:733199/1‑239 (MQ=255)

                                                                                                                                                 acaacCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCgcggtctcttataaacatc                                                                                       >  2:41220/1‑220 (MQ=255)

                                                                                                                                                      cGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAAATTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGCTGAAATCTCGGCGATGGCTGAAAAAGATGACGAAAGCGGGATCAAATACCTTGAAAAAAtaa                                                                                 >  1:293587/1‑237 (MQ=255)

                                                                                                                                                          cTGTCGCG‑TTTTAAAAAAAAATATGCACAACT‑ACAGG‑GAACTTATCA‑GGTTGATATCT‑GGCTAAAA‑AAAAAGAAGGTTTC‑ACAAAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTcc                                                                             <  2:1178372/239‑1 (MQ=255)

                                                                                                                                                             tCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGAGGACAATGACGGGATCAAACGGCTTGaaaaaaaaatacacgg                                                                          >  1:1004464/1‑229 (MQ=255)

                                                                                                                                                               gcg‑TTTTTCAAAAAAATATGCACAACT‑ACCTG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGAAGCAACAGTTTACGGTAGAACAACTACGTGAACTGGGTATTAAGGTGGATGAAATCCCGGCGCTGCCTGGAAAAGATGACGATACCGGGAGAAACACCCTTGAAAAAACAATCCCCGATa                                                                        >  1:1012974/1‑239 (MQ=255)

                                                                                                                                                                     tttCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGAGGAAATCCCGGCGCTGGCTGAAAAAACTGACGATAGCGGGGTCAACCCGCCTGACCAAACAATACCCggaaaaccg                                                                   >  1:1066747/1‑232 (MQ=255)

                                                                                                                                                                                       aaCT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCAGGCTCTGGCTGAAAAAGATGACGATAGCGGGATCAACTCGCTTGAACAAACCATTCCCGTAACAGACGCTGAATTTGATTTCaaa                                                 >  1:1223634/1‑238 (MQ=255)

                                                                                                                                                                                                aagAACTTACAA‑GTGTGATTTCT‑GGCGAAAA‑AAAAAGAAAGTTCC‑ACAAAAAATAATTACTTT‑AACCGCCAATGCAGAGCATCTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCAGGGTATTAAGGTGGATGAAATCCCGGCGCAGGCTTAAAGAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTTATTTCAATCATCAGc                                          <  2:135201/237‑1 (MQ=255)

                                                                                                                                                                                                    aCTTAACA‑GGTTGAAATCT‑GGCTAAAT‑AAAAAGAAGGTTTC‑AAAAAAAAAAACTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACt                                      <  2:1066747/239‑1 (MQ=255)

                                                                                                                                                                                                     cTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACtt                                     >  2:57590/1‑239 (MQ=255)

                                                                                                                                                                                                     cTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAACGTGATCAACTCGCTTGAACAAATCATTCCCGGTAAAGCTGCTGAATTTAATTTCaaaccacacaaactt                                     >  1:337822/1‑226 (MQ=255)

                                                                                                                                                                                                     cTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGCCTGAAAAAGATGACGTAAGCGTGATAAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTAAataaacacaaacta                                     >  1:1211652/1‑227 (MQ=255)

                                                                                                                                                                                                     cTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCCGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGaa                                                                                        >  2:269489/1‑188 (MQ=255)

                                                                                                                                                                                                     aaattaagaggttttttCT‑GGCCGAAAAAAAAAGAAGTTGTC‑ACAAAAAAAATTTACATT‑ATCCGCCAATGCAGAGCAACTTCTGCAGCCGCAGGTTACGGTAGAACTACTACGTGAGCTTGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAAc                                       <  1:103842/223‑1 (MQ=255)

                                                                                                                                                                                                      ttATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGACCAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCATTCATCAGCAACTTa                                    >  2:226200/1‑239 (MQ=255)

                                                                                                                                                                                                      gcgtaaggtgtgtatatgagcaagAAA‑AAAAAAGAGGTTGCAACAAAAAAAAATAACTTT‑AACCGCCAATGGAGGGCAACTTCGGCGGCCACAGGTTACGGTAGAACAACTACGTGAGCGGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAAc                                       <  1:631990/216‑1 (MQ=255)

                                                                                                                                                                                                       tATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCCTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGTTGCTGACTTTGATTTCAATCATCAGCAACTTaa                                   >  2:336305/1‑239 (MQ=255)

                                                                                                                                                                                                        aTCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAAt                                  >  2:571702/1‑239 (MQ=255)

                                                                                                                                                                                                        aTCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAAATCGCTTGAACAAATCATTCCCGGTACACCTGCTGAATTTGATTTCAATCATCAGCAACTTAAt                                  >  2:146932/1‑239 (MQ=255)

                                                                                                                                                                                                         tCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TCCCGCCAATGCAGAGCAACTTCTGCAGCCCCAGTTTACGGTAGAACAACAACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGCTAGCGTGATCAACTCACTTGAACAAATCATTCCCGGTACAGTCGCTGACTTTGATTTCAATCAACAACAACTTaaat                                 >  1:1289991/1‑237 (MQ=255)

                                                                                                                                                                                                              gTTGATATCT‑GGCTGAAT‑CAAAAGAAGGATTC‑ACAAAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGACGGAATCCCGGCGCTGGCTGAAACAGATGACGCTAGCGTGATCCACTCGCTTGAACAAATCATATCCGGAACAGCACCTAAATTTGTTTTACAACATACACAACTTAAtttaa                              >  1:1133520/1‑236 (MQ=255)

                                                                                                                                                                                                                  taatttttggcaaaaaaaaaaGAAGTTTTA‑AAAAAAAAAAATTACTTT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGc                            <  2:1211652/222‑1 (MQ=255)

                                                                                                                                                                                                                   tatCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGCGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGGGCTCAACTCGCTTGAACAACTCGTTCCCGGTACAGCTGCTGAATTTGATTTTAATCATCAGAAAATTAATTTGAGCATTc                        >  1:203995/1‑239 (MQ=255)

                                                                                                                                                                                                                         ggCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCTGCGCTGGCTGACAAAGCTGACGATAACGTGATCAACTCCCCTGACAAAATCATCACCGGTACCGCTCCTGAATTTGTTTTCAATAATCAGCAACTAAATTTGAGAATTCCCCaa                   >  1:657605/1‑239 (MQ=255)

                                                                                                                                                                                                                         ggCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCaa                   >  2:610042/1‑239 (MQ=255)

                                                                                                                                                                                                                             gAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACACCTGCGGAATTTGATTTCAATCAACAGAAACTTAATTTGAGAATTCCCCAAAtag               >  1:1303974/1‑237 (MQ=255)

                                                                                                                                                                                                                              gaaaaaaaatggtatttC‑AAAAAAAAGAATTAATTT‑CCCCGACAATGCAGGGCAAATTCTGGAGCCACAGTTTACGGTAGAACAACTTCGTGAGCTGGGTATTAAGGTGGATGAATTCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTg               <  1:798538/225‑1 (MQ=255)

                                                                                                                                                                                                                              act‑AAAAAGAAGGAATC‑ACAGTAAAAAATTACATT‑TACCGCCAATGCAGAGAAAATTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGATGGGTATTAAGGTGGCTGAAATGATGGCGCAGGCTGAAAAATATGACGATATCGTTATCGACTCGCTTGCACAACACCTACCAGGTACAGCTGCTGAATGAGATTTCCATCATCAGAAAATTAATTTAGCCATTCCCCAAATTga              >  1:1093818/3‑238 (MQ=255)

                                                                                                                                                                                                                              aaT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTGc              >  2:105885/1‑239 (MQ=255)

                                                                                                                                                                                                                               aaaaaaaaaGAGGTTTC‑AAAAAAAAAAAATTCATTGTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAAtt                <  2:1140211/235‑1 (MQ=255)

                                                                                                                                                                                                                                aaaaaaaaaaGGTTTC‑AAAAAAAAAAATTACATT‑TCCCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTGCa             <  2:907736/237‑1 (MQ=255)

                                                                                                                                                                                                                                aaaaaaaGAAGTTTTC‑ACAAAAAAAAATTACTTT‑CACCGCCAATGCAGGGCAACTTCTGCAGCCACAGGTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTGCa             <  1:1107462/237‑1 (MQ=255)

                                                                                                                                                                                                                                aaaaaaaGAAGGTTTC‑AAAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTGCa             <  2:810292/237‑1 (MQ=255)

                                                                                                                                                                                                                                        aatttttC‑AAAAAAAAAATTTACTTT‑AACCCGCAATGCAGGGACACTTCTGCAGCCAAAGGTTACGGTAGAACAAATACGGGAGCTGGGTAATAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTGCACTGTACCg     <  1:1282493/235‑1 (MQ=255)

                                                                                                                                                                                                                                         agGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTAAGCATTCCCCAAATTGCACTGTACCGt    >  2:749900/1‑239 (MQ=255)

                                                                                                                                                                                                                                         agGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGATGAATTTGATTTCAATCATCAGAAACTTAATTTGAGAATTCCCCAAATTGAACTTTaactt    >  1:561712/1‑235 (MQ=255)

                                                                                                                                                                                                                                         agGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAACAATTCCCGGTACAGCTGCTGCATTTGATTCCAATCATAAGCAACTAAACTTGCGCATTCCCCAAATTGCACTTTCCCg     >  1:117249/1‑238 (MQ=255)

CGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAAGTGG‑TATGCAAACAACCGATCTGTCGCG‑TTTTTCAAAAAAATATGCACAACT‑ACCAG‑GAACTTATCA‑GGTTGATATCT‑GGCTGAAT‑AAAAAGAAGGTTTC‑ACAGAAAAAAATTACATT‑TACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTTAATTTGAGCATTCCCCAAATTGCACTGTACCGTGA  >  NC_000913/3288842‑3289308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`