Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,492,827	G→T	13.2%	A88S (GCC→TCC)	tsgA →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,492,827	0	G	T	13.2%	204.6 / 3.4	89	A88S (GCC→TCC)	tsgA	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (9/66);  new base T (10/3);  total (19/70)
Fisher's exact test for biased strand distribution p-value = 4.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

TCACATGGATTAGCTTTCTCTCCTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGGCGCGCAGCATTGAGTGGTA  >  NC_000913/3492591‑3493053                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           tcacttgtttttgttttCTCTCCTTCGCACTATCTGGGTGTTTGTTTATTGTCACCGGGATGGTGAGTGGAAATCTCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAATAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCTTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑Tg                                                                                                                                                                                                                                            <  1:476331/223‑1 (MQ=255)    caTGGATTAGCTTTCTCTCCTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGCAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑C‑GGt                                                                                                                                                                                                                                     >  2:244031/1‑239 (MQ=255)          ttAGCTTTCTCTCCTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑C‑GGgtttgtg                                                                                                                                                                                                                               >  2:601634/1‑235 (MQ=255)            tgtttgttgtcCTTCGCACGTGCTGGGGTGTTGGTTAGTGTCCCCGGGAGGGTGAGGGGAAATCTCGGCGATTATTTCAATCTTCCTTTTTCCAGTATGAGTAAGACCTTCACCTTCCGCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGt‑t                                                                                                                                                                                                                            <  1:67952/230‑1 (MQ=255)            ggtttttTCTCCTTCGCACTGATTGGTGTGTTGGTTTTTGTCCCCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATTTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGt‑t                                                                                                                                                                                                                            <  1:583168/236‑1 (MQ=255)             gCTTTCTCGTCTACGCACTGACTGGTGCGTTGGGTATTGGCACCGGGATGGTGATGGGAAATATCGCCGATTATTGCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑Tc                                                                                                                                                                                                                           <  2:427469/239‑1 (MQ=255)                tttgttctgtcgcccgtagtgGTGCGTTGTTTGTGGTCCACGGGAGGGTGATGGGGAATGCCGCCGATTATTTCAATCGGCCGGTTTGCAGTATGATAAATACCTCCGCCTTCAGCAACGCCGGCCTTTTAATCGCTATCTGCCTCAACGCCTGGTTGATGGAAATCGTCCCGGTGAAAACGC‑AGTTACGTTTTGGCTTTCGCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGc                                                                                                                                                                                                                        <  1:244031/220‑1 (MQ=255)                  ctctcCTACGCACTGAACGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCca                                                                                                                                                                                                                      <  2:818593/239‑1 (MQ=255)                  ctctcCGACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCca                                                                                                                                                                                                                      <  2:989938/239‑1 (MQ=255)                  ctcccctaccgcatgactgGGTCGTTGGTTATTGGCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATTCCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCca                                                                                                                                                                                                                      <  2:453924/226‑1 (MQ=255)                  cgctacTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCca                                                                                                                                                                                                                      <  2:629468/234‑1 (MQ=255)                   tctcCTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTTTCCTGATGGTGCTGGCGGT‑TTC‑C‑GGGTTGATTT‑TGAGcact                                                                                                                                                                                                                     >  2:1098226/1‑236 (MQ=255)                   tctcCTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTgttgt‑ttataccc                                                                                                                                                                                                                     >  2:683901/1‑232 (MQ=255)                   tctcCTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGG‑TTC‑C‑GGgtttttttttacccac                                                                                                                                                                                                                      >  2:583168/1‑226 (MQ=255)                       cttccccttgactgggtttttGGTATTTGTCAACGGGATGGTGATGGTAAATTTCTCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGTCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑C‑GGTTTGATGT‑TCAGCCACAGcc                                                                                                                                                                                                                 <  1:441135/220‑1 (MQ=255)                       ctaccacctgactgGGGCGTTGGGTATGGCCCCCGTGATGTGGATGGGAAATATCCCCGTTTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCCCCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGcc                                                                                                                                                                                                                 <  1:1179562/232‑1 (MQ=255)                       ctaactactgacttGTTCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGcc                                                                                                                                                                                                                 <  2:78157/233‑1 (MQ=255)                       cTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGTTGGTGCTGTCGGT‑TGG‑C‑GGTTTTATGt‑tttaccccaca                                                                                                                                                                                                                  >  1:887663/1‑228 (MQ=255)                            gatttattgGTGTGTTTTTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCCCCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGAGGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGgcg                                                                                                                                                                                                            <  1:712613/232‑1 (MQ=255)                            ccctgactgGTGCGTTGGTTATTGTCACCGGGATGGTGATTGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGgcg                                                                                                                                                                                                            <  2:832313/237‑1 (MQ=255)                             actgactgGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATCCCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGgcg                                                                                                                                                                                                            <  2:1273526/238‑1 (MQ=255)                             actgactgGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGCAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGCTTGTTGT‑TCAGCCACAGcccgcggc                                                                                                                                                                                                           >  2:597496/1‑233 (MQ=255)                             actgactgGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGgcg                                                                                                                                                                                                            <  2:398101/238‑1 (MQ=255)                             actgactgGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGgcg                                                                                                                                                                                                            <  2:448681/238‑1 (MQ=255)                               tgactgGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑C‑GGGTTTATGT‑TTAGcccccaccctgcgccc                                                                                                                                                                                                         >  2:1153927/1‑225 (MQ=255)                                 actgGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑C‑CGTTTG‑TGTGTTAGCCCCAGCCCGGCGCtcgt                                                                                                                                                                                                       >  2:1264145/1‑236 (MQ=255)                                           tGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTGTGC‑C‑GGTTTG‑TGT‑TTCGCCACCGCCTCGGGCTTTTCTCT‑gcggcg                                                                                                                                                                                            >  2:927838/1‑239 (MQ=255)                                           tGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑CGTTTGTTGT‑TTTAGCACAACCCGGCGCTGTTTTCC‑gcggcg                                                                                                                                                                                            >  2:374345/1‑239 (MQ=255)                                                aTTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGtt                                                                                                                                                                                      <  2:197626/239‑1 (MQ=255)                                                     ccccGGGATGGTGATGGGAAATTACCGCGATTATTTCAATTTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCGCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATtc                                                                                                                                                                                 <  2:1194605/237‑1 (MQ=255)                                                        cGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGTCTTTCTCCTGATGGTGCTGGCGGT‑GTC‑C‑GGGTTTTTGT‑TTAGGCACACCCTAGCCCTGTTCTCT‑CCGGCG‑ATGttatgtctca                                                                                                                                                                              >  2:782036/1‑231 (MQ=255)                                                                  aTGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGGTTGGTGT‑TGTGCCACAGCCCTGCGCTCTTTTCG‑GCGGCGCGTGTTCTTTTTCggggggtgtg                                                                                                                                                                     >  2:1057055/1‑233 (MQ=255)                                                                     ggAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑CGGTTTTTGT‑TCACCCCCAGCCTCGCGCCGTTTTCG‑GCGGCG‑CTGtttttttttggggggggacgcg                                                                                                                                                                  >  2:49677/1‑218 (MQ=255)                                                                      gAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCgg                                                                                                                                                                 <  2:456044/238‑1 (MQ=255)                                                                         atatCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑CGGGTTTGTTGT‑TTGCCCCCACCCTGGCCCGTTTCTC‑‑GCGGCG‑GTGTTTATTTTCCggggggggcgccgcct                                                                                                                                                              >  2:484469/1‑228 (MQ=255)                                                                              gCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGCTC‑GGTGT‑ATGT‑TCAGCGCTACCCTGGTGCTGTTCTCGCGGGGGG‑CGGTTTATTCTGGGGGGGGCGAGCgggtgaacct                                                                                                                                                         >  1:175939/1‑231 (MQ=255)                                                                                    tttttcctttttccTTTTTCCATTTTGAGTTATTCCTTCCCCTTCCTTCACCCCGGCTTTTTAATCTCTATCGTCCGCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGAt                                                                                                                                                  <  1:601634/227‑1 (MQ=255)                                                                                    tattTCAATCTGCCTGTTTCCAGTATGATTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGGCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGAt                                                                                                                                                  <  2:1232236/239‑1 (MQ=255)                                                                                    tattTCAATCTGCCTGGTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTAGCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGAt                                                                                                                                                  <  2:899276/239‑1 (MQ=255)                                                                                         ccaTCTGCCTGTTTCCAGTATGAGTAATCCCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑a                                                                                                                                            <  2:66168/237‑1 (MQ=255)                                                                                             cTGCCTGTTTCCAGTATGAGTAATTCCTTCCCCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAt‑t                                                                                                                                       <  2:502406/239‑1 (MQ=255)                                                                                                ccTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑C‑GGtt                                                                                                                                                                                                                                    >  1:14803/1‑148 (MQ=255)                                                                                                ccTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGtt                                                                                                                                                                                                                                    <  2:14803/148‑1 (MQ=255)                                                                                                ccTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑gcggcg                                                                                                                                                                                            <  2:1031569/186‑1 (MQ=255)                                                                                                  tttttCCCGTTTGATTATTCCCTTCCCCTTCCCTAACTCCGGCATTTTAATCTCTTCCTTCCCCAACGCCGGGCTGATGGAAATCGTCCCGGTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTgg                                                                                                                                  <  1:1153927/237‑1 (MQ=255)                                                                                                  tGTTTCCAGTTTGAGTAATACCTTCACCTTCCTCAACGCCGGCATTGTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCTTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTgg                                                                                                                                  <  2:364767/239‑1 (MQ=255)                                                                                                     tttcgatATGAGTAATCCCTTCTCCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGGCTGGCTGATGGAAATCGTCCAGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGGTGGTGTGGGGGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCTCAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTaa                                                                                                                               <  1:941333/233‑1 (MQ=255)                                                                                                     ttccatgtttattAATACCTTCCCCTTTCCCAACGCCGGCCTTTTAAGCTCAGTCTCCCTCAGCTCCGTGCTGAGGGAATTCGGCCCGTTGAAAACGC‑ATTTACGTTTTGGCTTTCTCCGGATGGTGCTGGCGGT‑TGC‑T‑GGTTTGATGC‑TCAGCCTCAGCCTGGCTGTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCTGCATCACCATGTCGATTGCT‑ACTT‑TCCTGGTaa                                                                                                                               <  1:905513/227‑1 (MQ=255)                                                                                                     ttCCAGTATGAATAATTCCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCCTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTaa                                                                                                                               <  2:887663/239‑1 (MQ=255)                                                                                                       tcctttttgttattccctttcccttcCTCAACGCCGGCCTTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAaca                                                                                                                             <  1:584613/218‑1 (MQ=255)                                                                                                       tccttATGAGTAAGTCCTTCCCCTTTCGCAACGCCGGAATTTTAATCTCTTTCTGCCTCAACGCCTGGCTGATGGAAATCGGCCCGGTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCGGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAaca                                                                                                                             <  1:381031/235‑1 (MQ=255)                                                                                                       ccagttttggTAATCCCTTCACCGTCGTCAACGCCGGCTGTGTAATCTCTACCTTCACCAACGCCCTGTGGATGGAAATGTGCCGGTTGAAAACGC‑AGTTTCGTTGTGGCTTTCCCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGAAGT‑TCAGCCACAGCCTGGCGCTGTGCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCAACACCATGTCGATTGGT‑ACAT‑TCCTGGTAaca                                                                                                                             <  1:202806/230‑1 (MQ=255)                                                                                                        cattATGAGTAATTCCTTCCCCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAacac                                                                                                                            <  2:595673/236‑1 (MQ=255)                                                                                                         cttttGAGTTATCCCTTCCCCTTCCTCAAGGCCGGCATTTTAATCTCTATCTTCCTAAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAAcaca                                                                                                                           <  1:1187931/235‑1 (MQ=255)                                                                                                         ctgtttgattatctcctccctttcCTCAACGCCAGCATTTTAATCTCTATCTTCCCAAACGCCTGGCTGATGGAAATCGGCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAAcaca                                                                                                                           <  1:191496/218‑1 (MQ=255)                                                                                                         cgttttattattACCTTTCCCTTCCTTAACCCCGGCATTTTAATCCTTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAAcaca                                                                                                                           <  1:30506/228‑1 (MQ=255)                                                                                                           gactgttAATTCCTTCCCCTTCCCCAACGCCGGCATTTTAATCTCTATCTTCCTCACCGCCTGGCTGATGGAAATCGTCCCGTGGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAAcaca                                                                                                                           <  1:721091/231‑1 (MQ=255)                                                                                                           ccggaatAATCCCTTCCCCTTCCCCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAAcaca                                                                                                                           <  2:904946/231‑1 (MQ=255)                                                                                                            atttttttttcctttccttttcTCAACGGCTGGCTTTTCATCCTTTTCTTCCTAACTGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑ATTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAAcaca                                                                                                                           <  1:30771/215‑1 (MQ=255)                                                                                                            aTGAGTAATTCCTTCCCCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACaaa                                                                                                                         <  2:879712/238‑1 (MQ=255)                                                                                                            aTGAGTAACCCCTTCCTCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAAcaca                                                                                                                           <  2:1074688/236‑1 (MQ=255)                                                                                                               aGTAATTCCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAaca                                                                                                                             <  2:766392/231‑1 (MQ=255)                                                                                                                         tcaccttcCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TTC‑C‑GGCTTTTTGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGGGGTCAGCGGCATCACCACGTTGGTTGGTGACAT‑T‑CTGGTGAAACAAAAGTA‑TAAAGGGCgg                                                                                                          >  2:1100581/1‑238 (MQ=255)                                                                                                                         tcaccttcCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑ACGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGt                                                                                                          <  2:299619/239‑1 (MQ=255)                                                                                                                          caccttcCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAACCGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTc                                                                                                         <  2:796055/239‑1 (MQ=255)                                                                                                                              ttcCTTAACGCGTGCATTTTAATCTTTATCTCCCTCAGCTCCTGGCTGAGGGAAAGCGTCCCGTGGAAAACGC‑AGTTACGTTTTGGCTTTCTCCGGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAgcg                                                                                                     <  1:484469/239‑1 (MQ=255)                                                                                                                              ttcCTCAAAGCCGGCATTTTAATCCCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAgcg                                                                                                     <  2:1213598/239‑1 (MQ=255)                                                                                                                                ccctaaCGTCGGCATTTTAATCTCTATCTTCCGCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGcgcg                                                                                                   <  2:205685/235‑1 (MQ=255)                                                                                                                                   cAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGtt                                                                                                <  2:1101980/239‑1 (MQ=255)                                                                                                                                   cAACCCCGGCATTTTAATCTTTTTCTTCCTCAACGCCTGGCTTATGGAAACCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCCCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGtt                                                                                                <  2:1154152/239‑1 (MQ=255)                                                                                                                                        ccGGGATTTTAATCCTTTTCTCCCTTGCCTCCTGGGTTTTGGAAACCTCCCCTTTGAAAACGG‑TGTTCCGTTTTGGCTTTCCTCGGAGGGTGCTGGGGGT‑GTC‑C‑GGTTTGAGGT‑TCAGCCCCGGCCTGGGGCTGTTCTTG‑GCGGCG‑ATGTTAATGCTCGGGGTGGTAAGCGGCATCGCCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGc                                                                                           <  1:1141330/239‑1 (MQ=255)                                                                                                                                        ccGGCATTTTAATCTCTATCTTCCCCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGGGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGc                                                                                           <  2:65009/239‑1 (MQ=255)                                                                                                                                             ttttttatcttttctttCCCCAATGTCTGGCGTATGGAATCTGT‑CCGGTGAAAACGCAAGTTACTTTTTGTGTTCTTCCTGATGGGGGTGGCGGT‑TCG‑C‑GGTTTGATGT‑GCAGCCACATCCTGGCGCTGTTTTGG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCACCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTGCCCGCCTGtt                                                                                      <  1:1264145/224‑1 (MQ=255)                                                                                                                                                     ctctATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGcc                                                                                                                                                                                                                 <  2:1189419/113‑1 (MQ=255)                                                                                                                                                       ctttCTTCCCCAACCCCGTGCTTATGGAAATCGCCCCGGTGAAAACGG‑TGTTACGTTTTGGCTTTCCCCTGATGGTGCGGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCCCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGAc                                                                            <  1:904981/236‑1 (MQ=255)                                                                                                                                                        tATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑CGTTTTATGT‑TCAGCCCCAGCCTTGCGCTGTTCTCT‑GTGGCG‑AGGTTCTTTTTCGGGGGGGGGCGCGGGATCACCATGTCGGTTGGT‑ACAT‑TTCTTGTAACACAAATGTATTGAAGGG‑GGCAGCCCGGTTCCCCCCCTTTAtttttcccc                                                                            >  2:736094/1‑232 (MQ=255)                                                                                                                                                        aaccTTCCCCAACCCCGGGCTGATGGAACTCGTCCGGTTGAAACCGC‑AGTTCCGTTTTGGCTTTCGCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACCCAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACt                                                                           <  2:195896/236‑1 (MQ=255)                                                                                                                                                          tCTTCCTCAACGCTGGGGTGATGGAATTCGCGCGGGGGAAAACGC‑AGTTACTTTTTGGCTTTCGCCGGGTGGTGTGGGCGTG‑TGC‑C‑GGTTGGATGT‑TCGGTCACAGCCGGGTGTTGTTCTCG‑GCGGCG‑ATTTTCATCCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTcc                                                                         <  1:626139/239‑1 (MQ=255)                                                                                                                                                             tCCCCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGGTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCcttc                                                                      <  2:208152/239‑1 (MQ=255)                                                                                                                                                               cTCAACCCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCGGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCcttctt                                                                    <  2:1214340/239‑1 (MQ=255)                                                                                                                                                                                 gAAATCGTCCCGGTGAAAAGCC‑AGTTACTTTTTGGCTTGCTCCTGATGGTGGTGGCGGT‑TGC‑C‑GGTTGGATGT‑TCAGCCGCAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTTGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGgatgat                                                  <  2:641713/239‑1 (MQ=255)                                                                                                                                                                                   aatctcccctgtGAAAACGG‑GGTTGCGTTTTGGTTTTCCCCGGAGGGTGCTGGTGGT‑TGC‑G‑GGTTTGATGT‑TCAGCCACAGCCTGGCGTTGTTCTGG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGAtt                                                 <  1:736094/227‑1 (MQ=255)                                                                                                                                                                                                 aaaCGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATcgc                                   <  2:1026981/238‑1 (MQ=255)                                                                                                                                                                                                                  tggttcctccctgtgggTGCGGGGGGT‑GGC‑C‑GTTTGGATGT‑TCAGTCCCGGCGGGGCGTTGTTCGCG‑GCGGCA‑TTTTTCGTTCCCGGGGGGGTCGTGGGCTGCTCCGTGGGTGTTTGT‑‑CATATTCTGGTAAGTCAAAGGTA‑TGAGGGTCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGgcgc                  <  1:94051/224‑1 (MQ=255)                                                                                                                                                                                                                  tggtgtgctcCTGGTGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGTGGTTCTCG‑GCGGCG‑AGGTGCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGG‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGgcgc                  <  2:755720/232‑1 (MQ=255)                                                                                                                                                                                                                        tctctggtttgTGCTGGCGGT‑GGG‑T‑TGTTGTATGT‑TCCGCCACAACCTGGCGTGGTTCATG‑GCGGCG‑ATTGTCATTCCCGGGGGGGTCAGGGGCATCGCCATGTCGATGTGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGGCGCgcagca            <  1:374345/229‑1 (MQ=255)                                                                                                                                                                                                                             ttgtggttgtGGGGGT‑TTC‑C‑GTTTTGATGT‑TCAGCCACAGCCTGGGCTTTTTCCCG‑GCGGCG‑ATTTTCATTCTCGGGGGGGTCAGCGGCATCCCCATGTCGATTGGT‑ACAT‑TCCTGGTAACAAAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGGCGCGCAGCATTGAg       <  1:596468/230‑1 (MQ=255)                                                                                                                                                                                                                             tggtGGGGCTGGCCGT‑TGC‑C‑GGTTTGGTGT‑TCCGCCCACGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCCCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGGCGCGCAGCATTGAg       <  2:1198671/236‑1 (MQ=255)                                                                                                                                                                                                                             tGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGGCGCGCAGCATTGAg       <  2:898477/239‑1 (MQ=255)                                                                                                                                                                                                                                   tGCTGGCGGT‑GGC‑C‑GGTTTGAGCT‑TCAGCAAATGCCTGGCGCTTTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGGCGCGCAGCATTGAGTGGTa  <  2:660601/238‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           TCACATGGATTAGCTTTCTCTCCTACGCACTGACTGGTGCGTTGGTTATTGTCACCGGGATGGTGATGGGAAATATCGCCGATTATTTCAATCTGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGC‑AGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGT‑TGC‑C‑GGTTTGATGT‑TCAGCCACAGCCTGGCGCTGTTCTCG‑GCGGCG‑ATGTTCATTCTCGGGGTGGTCAGCGGCATCACCATGTCGATTGGT‑ACAT‑TCCTGGTAACACAAATGTA‑TGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCCTTCTTCAGTATGGCTGGGATGATTTTCCCAATGATCGCCGCGTTTCTACTGGCGCGCAGCATTGAGTGGTA  >  NC_000913/3492591‑3493053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑