Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,638,227	A→G	17.6%	E196G (GAG→GGG)	pitA →	phosphate transporter, low‑affinity, tellurite importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,638,227	0	A	G	17.6%	166.5 / 4.7	97	E196G (GAG→GGG)	pitA	phosphate transporter, low‑affinity, tellurite importer
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base G (10/11);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTCGAAGCTTACT  >  NC_000913/3637990‑3638460                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               tttaagagacctatcATTCGCTGATTTGCTGGAACGTCGGGGTTGGTTTAACCAATGGGTTGATAGCCGGGACGTCAGTTGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAg                                                                                                                                                                                                                                               <  1:236230/226‑1 (MQ=255)     aTCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑CGATCCACCTGACCCCAGCGGGGCGTg                                                                                                                                                                                                                                           >  2:871468/1‑239 (MQ=255)           ctctCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGAC‑T‑GAATTTTTTGCTGCGTCGCTACTTGAGCGGGA‑CCAAGAAACGCGCCC‑GGATCCACCTGACCCCAGCGGGGCGTG‑Aaaaga                                                                                                                                                                                                                                    >  2:750946/1‑239 (MQ=255)                aTACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGTTGGCGGTC‑T‑GATTTTTTTGCTCCGTCCCTACCGGGGGGGCA‑CCAAAAAAAGCCCCC‑GCTTCCCCCCCACCCCACGGGAGCGGG‑Caaaaaaaaag                                                                                                                                                                                                                               >  1:561066/1‑236 (MQ=255)                  aCGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACgg                                                                                                                                                                                                                             <  2:433329/239‑1 (MQ=255)                       tgttgggctgttgttcGGGTTTGGTTTAACCAATTCTTTGATGTCGGGGACGTCGGGGGGGGATGCACCCAATGCCCCAAAGGTTTTAAGTTTTTTCGTTTC‑T‑CTGATCGTTTCCCCTATTGCCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCCCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGa                                                                                                                                                                                                                       <  1:583755/225‑1 (MQ=255)                        aTTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATTCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑TATTTTTTTTCTCGTTCCCTCCCTGAGCGGCACCCGAGAAAC‑CACCC‑CTCTCCCCCCGCCCCCCACGGGGGGGGAAAAAAAAAGACGGC‑aaaa                                                                                                                                                                                                                       >  1:1027319/1‑237 (MQ=255)                        aTTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTTCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTTGCCACCTTACCCCACCGGAGAGTG‑AAAAGAAAAACggacaaca                                                                                                                                                                                                                       >  2:1220327/1‑233 (MQ=255)                        aTTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCCCCTGACCCCACCGGAGCCGG‑Aaaaga                                                                                                                                                                                                                                    >  2:236230/1‑226 (MQ=255)                        aTTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTTACCCCAGCGGGGCGTG‑AAAAGAAAGACGGC‑AAGaa                                                                                                                                                                                                                      >  2:577351/1‑239 (MQ=255)                        aTTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCCTCGCTACTGGAGCGGCA‑CCCAGAAACGCGCCC‑GTATCCACCTGGCCCCAGCAGACCGTG‑Aaaagaaatcaggccagaa                                                                                                                                                                                                                       >  2:153881/1‑228 (MQ=255)                          tGGCGCGATCATGGGGATTGGTTTAACCAATGGGTTGATGACCGGGACGGCAGTGGTGGATGCAATCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGaaaa                                                                                                                                                                                                                    <  2:1151622/239‑1 (MQ=255)                            gcgcGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTTGTGCGTCGCTACTGGAGCGGCA‑CCCAGAAACGCGCCA‑GTATCCACCCGTCCCCAGCGGAGCTGG‑CTAAGAAAGACGGG‑TAGaaaaa                                                                                                                                                                                                                   >  2:357580/1‑238 (MQ=255)                             cgcgATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCC‑CCAAGAAAAGCGCCC‑GTATCCCCCGTACCCCACCGGAGCCGG‑AAAAGAAAAAAGGC‑AAGaaaaaa                                                                                                                                                                                                                  >  2:127752/1‑237 (MQ=255)                                gATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GGTTTTCTTGCCGCTTTCCTCCTGGAGCGGGC‑CCAAGAAACCCGCCC‑CTATCCACCTGACCCCCGCGGGGGGGG‑AAAAAAAAAAACGC‑aaaaaaaacaccc                                                                                                                                                                                                              >  1:877036/1‑234 (MQ=255)                                  tcatcGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTGCCTACTGGAGCGGCC‑CCAAGAAACGCGCCC‑CGATCCACCTGACCCCAGCGGAGCGGG‑AAAAGAAAGACGGC‑AAGAAAAAcccccc                                                                                                                                                                                                             >  2:865673/1‑238 (MQ=255)                                     gggggttttttttAACCAAGTTGTTGATTCCCGGGCCCGTAGTGGTGGATGCACTCAATTCCCCGAAAGTTTTAGTTTTTTTGGGTTC‑T‑CTGATCGTTTCCCCTATTGCCGGCC‑TGGGTTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTc                                                                                                                                                                                                         <  1:32574/229‑1 (MQ=255)                                                        tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTT‑T‑GGTTTTTTTGCTTCGTCGCTAATGGGGCGGCA‑CCCAAACACGCGCCC‑GTATCCCCCCGCACCCAACGGAGGGTG‑AAAAGAAAAAGGGC‑AAGAAAAAGCCCCACGTCTGGGCCCGCGTTGCGCTGa                                                                                                                                                                                      >  1:1026336/1‑239 (MQ=255)                                                        tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTTTTGCTGCGTCGCTCATGGAGCGGCA‑CCACGAAACGCGCCC‑GTATCCCCCTGACCCCAGCGGGGCGGG‑AAAAGAAAAACGGC‑CAAGAAAAGCCCCCCTTcccgaccccgtttgggcga                                                                                                                                                                                       >  1:1241192/1‑220 (MQ=255)                                                        tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTTCTGCGTCGCTACTGGGACGGGC‑CCCAGGAACGCGCCC‑GTATCCACCTGACCCCCGCGGGGCGGG‑AAAAGAAAAACGGC‑GAAAAAAAAACCCCATCCTGGAcgcggcttcgggcga                                                                                                                                                                                      >  1:1056117/1‑228 (MQ=255)                                                        tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCCCCTGACCCCAGCGGAGCGGG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTTCGCTg                                                                                                                                                                                       >  2:1309831/1‑238 (MQ=255)                                                        tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGGCTT‑GTTTTTTTTCCTCGGTC‑CTCCCGGGGCGGGC‑CCAAGAAACGCGCCC‑CTGTCCCCCCTACCCCCGCGGGGCGGG‑AAAAGAAAAAACGC‑CAGGAAAAACCCCCGGCCTGGACGCGTATTgggcgct                                                                                                                                                                                      >  1:54221/1‑236 (MQ=255)                                                        tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCACGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGGGTTTGTTGGGGGTC‑T‑GATTTTCTTGCTTCATGGCTCTTGGCGCGGCA‑CACACAATCTCCCCC‑CAATCAACCCGACCCCCATGGAGGCGG‑GAAAGAAAAAAGCCAAAGAAAAAcccgcgctctgggcggcaatttagacaa                                                                                                                                                                                       >  1:986590/1‑216 (MQ=255)                                                              gatgaCCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCACGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAACAAAACGGC‑AAGAAAAAGCCGCCCTTCTGGACCCGCATTTCGCTGAACCtt                                                                                                                                                                                 >  2:705301/1‑238 (MQ=255)                                                                      ggACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTTTTGCTTAGTCGCTACTGGGGCGGCA‑CCAAGAAACGCGCCC‑GTATTCACCCGGCCCCAGCGGAGCGGG‑GAAAGAAAAACGGC‑CAGAAAAAAACGCCCGCCTGGACGGGGATTggcgggatacttttcccacac                                                                                                                                                                        >  1:1023573/1‑219 (MQ=255)                                                                       gACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑TATTTTCTTGCCGCGTCGCTACTGGGGCGGCA‑CCAAGTAACGCGCCC‑GTATCCACCTGACCCCCCCGGGGCGGG‑CAAAGAAAAACGGC‑AGGAAAAAACCACCGTCCCGGGCGCGTAATACGCGGACCCTTTcccacacag                                                                                                                                                                       >  1:472587/1‑232 (MQ=255)                                                                               gggtggtttcctctaattccccGAAGGTTTTAAGTTTTTTCGGTTT‑C‑TTGGTTTTTTCCCCTTTTTGGGGCC‑TGGTTTGTGCTGGCGGTC‑T‑GATTTTCTGGCGGCGGCCCTCCGGGAGCGGAA‑CCAAGAAACCGGCCC‑GTATCCCCCTGCCCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCgg‑cgtggcg                                                                                                                                                              <  1:144190/220‑1 (MQ=255)                                                                                      tGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGGGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCg                                                                                                                                                        >  2:652527/1‑238 (MQ=255)                                                                                      tGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCCTGACGCGTATTGCCGTGATCCTTTCCGCTATCGGCCGGGGGGTTTTc                                                                                                                                                         >  2:63646/1‑238 (MQ=255)                                                                                       ctcattattaccccGAAAGTCTTATGTTTTTTAGTGTC‑T‑CTGGACCTTTCCCCTATGGACGGCT‑GGGAGTTTGCT‑GCGGTG‑TGGATGTGCTTGAGGAGTGGCTACGGGAGGGTCA‑CCAAGACACGCCCCC‑GTATCCCCCTGGCCCAAGGGGGGCGTG‑AAAAGAAAGCCGGC‑AAGAAAACGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCa                                                                                                                                                      <  1:13932/228‑1 (MQ=255)                                                                                        ctcTCATTACCCCAAATGTTTTAAGTTTTTTGGGTTC‑T‑TCGTTCTTTTCCCTTTGTGTCGGCT‑GGGTTTTTGCTGGCGGTT‑T‑GATTTTCTTGCTGCGTCGCTACGGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGCCCCCAGCGGGGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCAc                                                                                                                                                     <  1:652527/237‑1 (MQ=255)                                                                                         aCTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTTTTTCTGCGTCGCTACTGGAGCGGCC‑CCCAAAAACGCGCCC‑GTATCCACCTGACCCCCGCGGAGCGGG‑GAAAGAAAGACGGC‑CAGAAAAAACCCCCCTTCTGGCCGCGGCATGCGCTGATCCTTTCCCCTATCCG‑AGTGGGGgttttctccg                                                                                                                                                    >  1:1030623/1‑233 (MQ=255)                                                                                           tCAATTCCCCGAAATTATTAAGTTTTTCCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGgc                                                                                                                                                  <  2:472587/239‑1 (MQ=255)                                                                                                  cccGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAACCCCCGTTCTGGACGCGGATTGCGCTGATCCTTTCCCCCATCGG‑CGTGGGGCTTTCCCGCCGCGCGCACg                                                                                                                                           >  1:997232/1‑239 (MQ=255)                                                                                                  cccGAAAGTATTAAGTATTTGCGGTTC‑T‑TTGATCGTTCCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACg                                                                                                                                           <  2:1030623/239‑1 (MQ=255)                                                                                                  ccagAAAGTTTTATGTTTTTTGGGGTCTT‑CTG‑TCTTTTCCCCTTTTTGCGGCC‑TGGTTGTTGCGTGGGGTC‑T‑TATTTTTCTCACGCGCCGCTACGGGAGCGGCC‑CCAAGAAACGCGCCC‑GTATCCACCTGGCCCCAGCGAGGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACg                                                                                                                                           <  1:945472/236‑1 (MQ=25)                                                                                                   ccGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGa                                                                                                                                          <  2:997232/239‑1 (MQ=255)                                                                                                       aagtattaagtattTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGGGTTTTTGCACGGGGCGCACgcgtg                                                                                                                                       >  2:864261/1‑234 (MQ=255)                                                                                                         gtattaagtattTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCCACTGGGCCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCCGCGGCGCGTG‑CAAAGAAAAAAGGC‑ACACAAAAACCGCCGGTCTGGACGCGGATTGCGCGGACCCTTTTCCCCATCGG‑CGCGGGGTGTTCGCAAGTCGCGCAcacaaggga                                                                                                                                    >  1:717886/1‑231 (MQ=255)                                                                                                           attaagtattTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTTCTGCGTCGCTACTGGAGCGGCA‑ACCAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCCCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTTTCACGGCGCGCACGATGGGCAGa                                                                                                                                  >  2:415820/1‑239 (MQ=255)                                                                                                                   tttttgcggttcttgtgtcttttCCCCTTTGGGTGGCC‑GGGTGTTGGCTGGGGGTC‑T‑GATTTGTTTGCTGCGTCACTAGTGGAGCGGCA‑CCAAAAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGA‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCa                                                                                                                            <  1:815536/219‑1 (MQ=37)                                                                                                                   ttttTGGTTT‑TCCTGATCTTTTCCCTTATTGGCGGCC‑TGGGTGTTGCTGGCGGTC‑T‑TATTTTCTTGCTGCGTAGCTACTGGAGGGGCA‑CCAAGAAACGCGCCC‑GTATCCCCCTGACCCCAGCGGCGCGTG‑AAAAGAAAGACGGA‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAt                                                                                                                           <  2:744278/239‑1 (MQ=37)                                                                                                                    ttttttgtttttttttttgTTTCCCCTTTTGGCGGCCGTGGT‑TTTCCTGGCGGTC‑C‑TCTTTTCTTGCGGCGTCGCTCGTGGGGCGGCC‑ACAAGAAACCCGCCC‑GTATCCACCTGACCCCAGCGGGGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAt                                                                                                                           <  1:889604/221‑1 (MQ=25)                                                                                                                    tttCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑ACAAGAAAGACGGC‑CAGAAAAAGCCGCCGTTCCGGACGCCGATTGCGCTGATCCCTTCCGCTATCGG‑CGTGGCGGTTTTGCGCCGCCGGAACAATGGTAAGAAAGGGAT‑Tg                                                                                                                        >  1:394701/1‑239 (MQ=255)                                                                                                                      tCGGTTC‑C‑CTGATCGTTCCCCCTATTGGCGGCC‑TGGGGTTTGCCGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACGGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGt                                                                                                                      <  2:54221/239‑1 (MQ=255)                                                                                                                      tCGGTCC‑T‑CTTATCGTTTCCCCTATTGGCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGt                                                                                                                      <  2:831969/239‑1 (MQ=255)                                                                                                                       ttcgtttttttgtttgTTTCCCCTTTGGTCGGCT‑GGGTGTTTGGTGGCGTTG‑T‑GTTTTCCTGGCTGCGGCACTGCTGGGGGGGCA‑CAAAAAAAC‑CGCCCATTATCCACCGTACCCCGGCGGGTCGCG‑AAAAGAATGCCGGC‑AAGAAAACGCCGCCGTTCTGGCCGCGTGTTGCGCTGATCCTTTCCGCTATCGG‑CTGGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGTCAT‑Tgg                                                                                                                       <  1:1220327/224‑1 (MQ=255)                                                                                                                           tc‑t‑cTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGGGGTTTTTCCCGGGGCGGAACATGGGGAGAAAGGCAT‑TTGTCCGGt                                                                                                                 >  2:889604/1‑239 (MQ=255)                                                                                                                                  gttcttttCCCCTTTTGGCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGCCGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGt                                                                                                            <  2:492960/234‑1 (MQ=255)                                                                                                                                  gATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGGGGTTTTTCCCGGGGCGCACCGTGGTCCGAAAGGCAt‑ttggtcggttttgt                                                                                                            >  2:541784/1‑226 (MQ=255)                                                                                                                                       tttCCCCTTTTGGCGGCC‑TGTGGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTGCTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTa                                                                                                       <  2:1026336/239‑1 (MQ=255)                                                                                                                                         tCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑CAAAGAAAGAGCGC‑AAGAAAAAACCGCCGTTCTGGACCCGTATTGCGCTGATCCTTTCCGCTATAGG‑CGAGGCGTTTTCTCACCGCGCGCACGCGGGTCAAACAAGCATATGGGCGGGTTTTG‑TGGTatt                                                                                                     >  1:53405/1‑239 (MQ=255)                                                                                                                                                      gCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGGGTTTTCGCCCGGCGCGGACGATGGGCAGAAAGGCCT‑TGGTCTTGGTATTTTGGGattgttttggcgccc                                                                                         >  2:19328/1‑230 (MQ=255)                                                                                                                                                        c‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGGATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGGTTTTGCACGGGGCGGAAGATGTTCCGAAAGGCCT‑TGGGCTGGGTTTGTTGGGATTGATTGGGGTCtcgc                                                                                       >  2:751095/1‑238 (MQ=255)                                                                                                                                                           ggTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGGTTTTGGCCGGGGCGGACGTTGGGACGAAAAGCAT‑TTGGCTGGGTTTGTTGGTATTTTTTTgcgccgcgccg                                                                                     >  2:81114/1‑237 (MQ=255)                                                                                                                                                            gtggTTGCGGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCcagc                                                                                   <  2:438751/239‑1 (MQ=255)                                                                                                                                                             tgttgggggggggTT‑T‑GCTTTTCTTGCGGCGTCGCTCGGGGGGGGTCA‑CCAAGAAACGCGCCC‑GTATCCACTGGCCCCTGGCGGAGCGTG‑AAAAGAAAGAGGGG‑AAGAAAAGGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCcagc                                                                                   <  1:871468/227‑1 (MQ=37)                                                                                                                                                                     ggCGGTC‑T‑GATTTTCTTGCTGGGTCGCTACGGGAGCGGCA‑CCAAGAACCGCGCCA‑GTATCCCCCTGACCCCAGGGGAGCGTG‑AAAAGAAAGACGGA‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCg                                                                           <  2:596091/238‑1 (MQ=255)                                                                                                                                                                       cGGTC‑T‑GATTTCTTTGCCGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCCCCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCgtg                                                                         <  2:479078/238‑1 (MQ=255)                                                                                                                                                                             ttttttttCTGGCTGGGTCGCTGCTGGGGGGGCA‑ACAAAAACCGCGCCC‑GTACCCACCGGCCCCCAGGGGGGCGTG‑AAAAGAAAGCCGGC‑AAAAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGa                                                                     <  1:63646/234‑1 (MQ=37)                                                                                                                                                                             t‑GATTTTCTTGCTGGGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGCCCCCAGGGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAAc                                                                   <  2:1023573/239‑1 (MQ=255)                                                                                                                                                                                aTTTTCTTGCTGCGTCGCTACGGGAGGGGCC‑CCAAGAAACGCGCCC‑GTATCCCCCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGA‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACAt                                                                 <  2:77115/239‑1 (MQ=255)                                                                                                                                                                                           gggcccgtACTGGAGGGGAA‑CAAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACt                                                       <  2:668583/231‑1 (MQ=255)                                                                                                                                                                                               cgccacggggacGGCA‑CCAAGAAACGGGCCC‑GGTGCCACCTGACCCCAGCGGAGCGTA‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCt                                                   <  2:394701/227‑1 (MQ=38)                                                                                                                                                                                               ccgtcgtGGAACGCCA‑CCAAGAAACGCGCCC‑TTATCCACCGGACCCCAGGGGGGCGTG‑AAAAAAAAGCCGGC‑AAGAAAAAGCCGCCGTTCTGGGCGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCt                                                   <  2:891560/232‑1 (MQ=38)                                                                                                                                                                                               cGCTACTGGAGCGGCA‑CAAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCt                                                   <  2:122315/238‑1 (MQ=255)                                                                                                                                                                                                gCTACTGGAGCGGCA‑CCAAGAACCGCGCCC‑GTACCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGA‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTa                                                  <  2:957169/238‑1 (MQ=255)                                                                                                                                                                                                    cgggAGCGCCA‑CAAAGAACCGCGCCC‑GTACCCACGTGACCCCAGGGGAGCGTG‑AAAAGAAAGACGGC‑AAAAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGaaa                                             <  2:96416/237‑1 (MQ=255)                                                                                                                                                                                                      ggAGCGGCC‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGGGGAGCGTG‑AAAAGAAAGGCGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATc                                           <  2:948669/239‑1 (MQ=255)                                                                                                                                                                                                      ggAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGGTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TTGTCTGGGTTTGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGGACATGAATGCCCCCGGCCACGAAATc                                           >  2:432223/1‑239 (MQ=255)                                                                                                                                                                                                       gcgCGGCA‑CCAAAAAGCCCGCCC‑GTACCCCCCTGCCCCCAGGGGGGTGTG‑AAAAAAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATc                                           <  1:577351/236‑1 (MQ=38)                                                                                                                                                                                                         gCGGCA‑ACAAGACACGCGCCC‑GTATCCGCCTGCCCCGAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCac                                         <  2:599084/238‑1 (MQ=255)                                                                                                                                                                                                            gCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCgg‑cgtgg                                                                                                                                                                >  2:555519/1‑117 (MQ=255)                                                                                                                                                                                                            gCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACg                                                                                                                                           <  1:860094/138‑1 (MQ=255)                                                                                                                                                                                                            gCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACg                                                                                                                                           >  2:860094/1‑138 (MQ=255)                                                                                                                                                                                                            gCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCa                                                                                                                            <  2:114603/153‑1 (MQ=255)                                                                                                                                                                                                            gCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCa                                                                                                                            >  1:114603/1‑153 (MQ=255)                                                                                                                                                                                                            gCA‑CAAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCgg‑cgtgg                                                                                                                                                                <  1:555519/117‑1 (MQ=255)                                                                                                                                                                                                                   aGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGACGCGGCCGCTGGGCAGAAAGGCAT‑TGTGTTGGTTATGTTGGTATTGAATTGCGTCGCGCAACCAGGGCTCAGGGGGAACAGGAATGCGACAGGCTAAAAAATCaaccggaccggg                               >  1:470920/1‑236 (MQ=255)                                                                                                                                                                                                                      aaCGCCCCC‑GTACCCACCTGGCCCCAGCGGAGCGTG‑AAAAGAAAGCCGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGAt                            <  2:561066/239‑1 (MQ=255)                                                                                                                                                                                                                        cgcgCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGGCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGCAATCACCCGTACCCCGGATg                           >  2:57521/1‑238 (MQ=255)                                                                                                                                                                                                                         cgcccc‑GTAACCCCCCGGCCCAAGCGGGCGGTG‑AAAAAAAATACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTACTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGcc                         <  2:470920/236‑1 (MQ=37)                                                                                                                                                                                                                           gcCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTTTGTTGGGATTGATTGGCGTGCCGCCCGCAGGCTTCGTGGGGAACAGGAATGCCCCTGGCTCCGAAATCacccgaacccgggtagcaac                       >  1:1019769/1‑230 (MQ=38)                                                                                                                                                                                                                            ccc‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGGATTGATTGGCGTCGCGCCCGCAGGCTTCCGGGTGAACATGAATGCCCCTGGCTCAGAAATAACCCGAACCCGTGATGCCATc                      >  2:869550/1‑239 (MQ=38)                                                                                                                                                                                                                                      cccggACCCTAGGGGGGCGTG‑AAAAGAAAGCCGGA‑AAGAAAAGGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTc            <  2:1039620/235‑1 (MQ=255)                                                                                                                                                                                                                                      aCCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCCCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGGATTGATTGGCGTCGCGCCAGCAAGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGGGATGCCCTC‑AACAACGTc            >  2:339714/1‑239 (MQ=255)                                                                                                                                                                                                                                      aCCTGACCCCAGCGGAGCGTA‑AAAAAAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTc            <  2:838459/239‑1 (MQ=255)                                                                                                                                                                                                                                      aCCGGACCCCAGCGGAGGGTA‑AAAAGAAAGGCGGA‑AAAAAAAAGCCGCCGTTCTGGGCGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTc            <  2:1263489/239‑1 (MQ=255)                                                                                                                                                                                                                                        cTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGCTGGTCAGAAAGGCAT‑TGGGCTGGTTATGTTGGTATTGATTTGCGGCGCGCCCGCAGGCTTCCCGCGTAAAATGGATGCCACTGGTGCCCGAATAACCCATACCCGCTAAGCCCCc‑aacaaacacga          >  1:128402/1‑233 (MQ=25)                                                                                                                                                                                                                                        cTGACCCAAGCGGAGCGTG‑AATAGATAGACGGC‑AAGAAAAACCCTCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATAGG‑CGTGGCGTTTTCGCCCGGCGCGAACGACGGTCAGAAAACCAT‑TTGTCCGGTTTTATTGGTATTTAATGACGTCTCGCCGCCAAGGTCCGCTGAGAAGACGAATTCCCCTGGCTGCCAAAACACCCGTGCCCGTGCTGCCACCAAACAACCTCg           >  1:276314/1‑239 (MQ=255)                                                                                                                                                                                                                                          gACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGCGGCGTTTTCGCACGGCTCGAACGATGGGCACAACAGCAT‑TGGTCCGGTTATTTTGGTATTCCATGGCGGCGTGCCACCCGGCCTCTTGGGGAACATGAATGTCACTGGACACGCCAGCaccccgacccggcgatcccataaaaacaaccga          >  1:627889/1‑216 (MQ=255)                                                                                                                                                                                                                                           gccccGGGGGAGGGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGGATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTCGAAGc       <  2:588428/238‑1 (MQ=255)                                                                                                                                                                                                                                           aCCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGTTGCCCTC‑AACAAAGTCGac         >  2:1196300/1‑236 (MQ=255)                                                                                                                                                                                                                                            ccccGGCGGTCCGTG‑AAGAGAAGGCCGGC‑AAGAAAAGGCCGCCGTTCGGGACGCTTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTCGAAGc       <  2:377017/238‑1 (MQ=255)                                                                                                                                                                                                                                            ccccAGGGGGGCGTA‑AAAAGAAAGGCGGC‑AAGAAAAAGCCGCCGTTCTGGACGGGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTCGAAGc       <  2:1027319/238‑1 (MQ=255)                                                                                                                                                                                                                                               cAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGGATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATGACCCCTACCCGTGATGCCATC‑AACAACCTCGAAACtt     >  2:1078507/1‑237 (MQ=255)                                                                                                                                                                                                                                                   ggAGCGTG‑GAAAGAAAAGCGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTGCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTCGAAGCTTACt  <  2:100506/236‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               CTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTC‑T‑CTGATCGTTTCCCCTATTGTCGGCC‑TGGTGTTTGCTGGCGGTC‑T‑GATTTTCTTGCTGCGTCGCTACTGGAGCGGCA‑CCAAGAAACGCGCCC‑GTATCCACCTGACCCCAGCGGAGCGTG‑AAAAGAAAGACGGC‑AAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGG‑CGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCAT‑TGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACTGGCTACGAAATCACCCGTACCCGTGATGCCATC‑AACAACGTCGAAGCTTACT  >  NC_000913/3637990‑3638460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑