BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,926	T→A	9.5%	T110S (ACC→TCC)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,926	0	T	A	9.5%	301.5 / 3.6	109	T110S (ACC→TCC)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (72/21); new base A (6/7); total (78/31)
Fisher's exact test for biased strand distribution p-value = 3.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAAT  >  NC_000913/4241689‑4242163

cgcaaCGGGAAAAAAAATATTTTACAAAATAACAAAAAGGTTGCCAGAAAATAGCCGC‑AACTG‑GTTAAAAAGAGA‑CCAGTGC‑CCCGCGAATATGG‑GATGGCGCGA‑GTC‑CCGCAAAATACATGGGCAAGAG‑AATTGAGGCTAACCCAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTa                                                                                                                                                                                                                                                      <  1:268372/236‑1 (MQ=255)

cGGAACGGGAAATAAAATGGTTACCGCAGTAAACTAAAAGTGGCCAGAAAAAAACCGCAAACTG‑GTT‑AAAAGAGC‑CCAGTTC‑CCCGCAAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATGGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTa                                                                                                                                                                                                                                                      <  2:551030/239‑1 (MQ=255)

       ggCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATACCAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AAAGGTAAAA‑CCaa                                                                                                                                                                                                                                              >  2:379711/1‑239 (MQ=255)

        gCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAACTAATCGCAAACTG‑GTT‑AAACGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCAA‑GTT‑CAGCAATATACATTGGCGAGCG‑CATTGTGGCTAAACCAAGGCCAATAACGCCATTAATGCGATAAATAACAAATTCC‑TGAGCAT‑AACCTTCCAGAA‑ACACAGGCAAAATTATGTACGGGGTT‑AT‑AAAGGAAAAA‑ACaaa                                                                                                                                                                                                                                             >  1:375059/1‑238 (MQ=255)

          aaTAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGGTT‑AT‑AGAGGTAAAA‑CCAAGt                                                                                                                                                                                                                                            >  2:289514/1‑238 (MQ=255)

          aaTAAAATAGTGAACGAAGTAAAATAAATGTTGCACAAAAATAACCGAAAACGG‑TTT‑AAAAGAGC‑CAAGTTC‑CCCGCGAATAGGA‑GCTGGAGCAA‑GTT‑CCGCAATATACATGGGCAAGAG‑CATTGAGGCTAAACCAACGCCAAGTCCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGt                                                                                                                                                                                                                                            <  2:795837/238‑1 (MQ=255)

                  aGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGCAAT‑AACCTGCCAGAT‑AAAAAGGACAAGGGTTGTCCGGGGTT‑AA‑AAAGGAAAAA‑ACAAATTCTGG‑CCA‑g                                                                                                                                                                                                                                 >  1:1202313/1‑239 (MQ=255)

                     tttccaaaaaaaCAAAAAGTTGCCCAAAAAAAAACCGAAAATG‑GTT‑AAAA‑AGACCCCGTTT‑CCC‑CGGAAATGG‑GGCGGGGCCACAGG‑CCGCAATAATAATTGGGGGGAGACATGGAGACTAACCCACCTCCATACCCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑c                                                                                                                                                                                                                              <  1:20380/230‑1 (MQ=255)

                     aaggagacaaaaaaaaaaGTTGCCCGAAAATAACCGCAAAGTG‑GTT‑AAAAGACA‑CCATTTC‑CCCGGAAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑Ca                                                                                                                                                                                                                             <  2:559426/225‑1 (MQ=255)

                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCCCAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAg                                                                                                                                                                                                                            >  2:50117/1‑239 (MQ=255)

                           aGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAA‑AACCTGCCAGAT‑AAACAGGCACCGGGTTATCCGTGGTT‑TT‑AAAGTAAAAA‑CCAAATTCTGG‑CCA‑ggacgaaaca                                                                                                                                                                                                                        >  1:1216839/1‑231 (MQ=255)

                             tAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CTGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTa                                                                                                                                                                                                                      >  2:1172038/1‑238 (MQ=255)

                             tAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTac                                                                                                                                                                                                                     >  2:216191/1‑239 (MQ=255)

                                  tAAAGGTTGCACAAAAATAACCG‑AAACGGTGTT‑AAAAGA‑A‑CCCGTTTCCCCGGAAATAGGG‑GCGGGGCCCA‑TTC‑CCGCAATATACTTTGGGGAGAG‑AATTGGGGCTAAACCACCGCAAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAg                                                                                                                                                                                                               <  2:755422/239‑1 (MQ=255)

                                  aaaaaGTGTCCCAAAAAAAACCGCAAACTG‑GTT‑AAAAAAAA‑CAATTTC‑CCCGCAAATTAGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAg                                                                                                                                                                                                               <  2:1163924/238‑1 (MQ=255)

                                   taatgtttccataaaaTAAACACAAAGTG‑TTT‑AAAAGAGA‑CCCGTTT‑CCCCAGAAATGGAGGCGGGAG‑CA‑GTTCCCTAAATATAAATGGGAAAGAG‑AATTGAGCATACACAACCACAAATACCGCCAATAATGCGATAAATAACAAATGCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAg                                                                                                                                                                                                               <  1:284972/227‑1 (MQ=255)

                                     aaGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCAGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑AAGaaa                                                                                                                                                                                                            >  2:721917/1‑239 (MQ=255)

                                       gTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCATTACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AACCAGGCACAGTGTTTTTCGGGCTT‑CA‑AAAGGAAAAC‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTAAAC‑CAGAAATa                                                                                                                                                                                                          >  2:1035054/1‑239 (MQ=255)

                                         tGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCACTACCACCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑CACCTGCCCGAT‑AAACAGGCACCGTGTTGTCCGGGTTT‑AT‑AGCGGTAAAA‑CCAACTGCTGA‑CCA‑GG‑CAGAACCTACAC‑CAGAAATaaa                                                                                                                                                                                                        >  2:609128/1‑239 (MQ=255)

                                         tGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑ACAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCCATATACATTGGCGCGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑CT‑AGAGGTAAAA‑CCCAGTTCTGG‑CCA‑TG‑CGGACCCTACTC‑CAGAAGTaaa                                                                                                                                                                                                        >  2:1177958/1‑239 (MQ=255)

                                          gCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCCGAT‑AAAAAGGCACAGAGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑ACAAAATCAGA‑CCA‑GG‑AAAAACCTaaaacctgtcgctta                                                                                                                                                                                                        >  1:559426/1‑225 (MQ=255)

                                          gCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACCGTCACAGCGTTGTCCGGGTTT‑AT‑AGAGGAAAAA‑ACAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATaaaa                                                                                                                                                                                                       >  2:615839/1‑239 (MQ=255)

                                          gCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AAAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATaaaa                                                                                                                                                                                                       >  2:168986/1‑239 (MQ=255)

                                          gCCCAAAAATAATCGCAACCTG‑TTA‑AAAAGAGA‑CCAGTTC‑CCCGAGAATATGA‑GCTGGACCCA‑GTC‑CCGCAATATCCATGGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATaaaa                                                                                                                                                                                                       <  2:1202313/239‑1 (MQ=255)

                                           cccGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGACCCAACAC‑CAGAAATaaaaa                                                                                                                                                                                                      >  2:508295/1‑239 (MQ=255)

                                           cccGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTCCCAGAT‑AAACAGGCACAGTATTGTCAGGGTTT‑AT‑AAAGGAAAAA‑CCAAGTCCTGG‑CCC‑GG‑CGGGACCACAAC‑CAGAaaaaaaaa                                                                                                                                                                                                      >  1:441508/1‑239 (MQ=255)

                                                aaaaatatccacatttttttaaaaaGA‑A‑CAAGTTC‑CCCGAGAAAAGGA‑GGTGGCTCCA‑GCC‑CCGCAATATGATTTGGCGAAAG‑CATGAAGGTTAAACACACCAAAACTCCGCCAATAATGCGATAAATAACAAAATCC‑GGGACAT‑AACCTGCCATAT‑TAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑g                                                                                                                                                                                                <  1:73135/218‑1 (MQ=255)

                                                                 gTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTaa                                                                                                                                                                               >  2:180701/1‑239 (MQ=255)

                                                                  gaaaaaaGAGC‑CCGGTCT‑CCCTCGAATTGGA‑GCTGGAGCCA‑TGT‑CCGCAATACTCATGTGCGAGAG‑GATTGAGCCTAAACAACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGGAAAA‑CCAATTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGCCAGCAGCAATCTTaa                                                                                                                                                                               <  2:773422/236‑1 (MQ=255)

                                                                            a‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTaaagaaataaag                                                                                                                                                                     >  1:789744/1‑229 (MQ=255)

                                                                            a‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCacg                                                                                                                                                                     >  2:82982/1‑239 (MQ=255)

                                                                            a‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCacg                                                                                                                                                                     >  2:1120889/1‑239 (MQ=255)

                                                                             gccAGTTA‑CCCGCGAATATGG‑GCTAGGGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACACCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑TG‑CGGACCCTACCC‑CAGAAATAAAAAAC‑A‑GGCAAACAACAATCTTAAggaaatacca                                                                                                                                                                     >  1:166109/2‑230 (MQ=255)

                                                                                          cgAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGCGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑AGACAGCAGCAATATTAAGTGAATCacgaaacccgaaacg                                                                                                                                                         >  1:789078/1‑228 (MQ=255)

                                                                                             atatGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACGACGACCGAAg                                                                                                                                                           >  2:93816/1‑234 (MQ=255)

                                                                                              taggA‑GCTGGAGCAA‑GTC‑CCGAATAATAAATTGGCGGGAG‑CATTGAGGTTAACCAAACGCCAATCCCGCCAATAAGGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                                                                                                                                     <  2:703329/236‑1 (MQ=255)

                                                                                              atatgaatggtcgCCA‑GTT‑CCGCAATATACATGGGGGAGAG‑CATGGAGCCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGtt                                                                                                                                                      <  2:795552/227‑1 (MQ=255)

                                                                                                    gCTGGAGCCA‑GTT‑CCGAAATATACATTGGGGAGAG‑TATTGAGGCTAACCCAACCCAAAAACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGc                                                                                                                                                 <  2:1216839/238‑1 (MQ=255)

                                                                                                        gAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                                                                                                                             >  2:763991/1‑238 (MQ=255)

                                                                                                          gCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGAcagca                                                                                                                                                                                         >  2:551890/1‑192 (MQ=255)

                                                                                                                tt‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTTACTGCAATAACCACCGa                                                                                                                                     >  2:435445/1‑239 (MQ=255)

                                                                                                                tt‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGCTTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACATCAGCAACCTTAAGTGAACCACCATACCCGAAGCGGTTACTGCAATAAACACCgc                                                                                                                                     >  2:511262/1‑238 (MQ=255)

                                                                                                                      cAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAACTAAAAAAA‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTATTGCAATAACCACCCAGGGc                                                                                                                                 >  2:556009/1‑238 (MQ=255)

                                                                                                                       aaTATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGAAAGCAGCAATCTTAAGGTAATCACGACGACCGAAACGGTAACTGCAAAAacacacaagggga                                                                                                                                >  1:739997/1‑227 (MQ=255)

                                                                                                                         tataCATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGCTAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CACAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACCACGACCGAAGCGCTTACGCCCATAAACCCCAAGGGCAcc                                                                                                                              >  1:761977/1‑238 (MQ=255)

                                                                                                                           catgtatggggagaattttGAGAATAACCCAACCACAACCCCACAAATAAGGCAAAAAATAA‑AAATTCCGGGGACAA‑AACCTGCCAGAT‑AAACGGGCAAGTTGTTGCCCGGGTTT‑AA‑AGAGAAAAAA‑CCAAGTTCCGG‑CCA‑GG‑CAGACCCTACCC‑CAAAAAAAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGCCGCCCGAGGCGGTTCCTGCAATACCCACCGAGGGCAccgc                                                                                                                            <  1:1120889/222‑1 (MQ=255)

                                                                                                                              aTTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAaccctgtatcttatacaaat                                                                                                                         >  2:1303474/1‑221 (MQ=255)

                                                                                                                                tGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                                      >  2:1243231/1‑239 (MQ=255)

                                                                                                                                tGGCGAAAG‑CATTGAGGCTAAACCAACGCCAATACCGCAAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTCGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CACAACCTACAC‑CATAAATAAAAAAC‑A‑GGACAGCACCAATATTACGTGAATCACGACTACCGAACCGGTTACCGCCAAAACCACCGAGGGCAACCCCgaaga                                                                                                                      >  1:730262/1‑239 (MQ=255)

                                                                                                                                   ggagaG‑TATTGCGCCAAAACCACCGCAAACCCCGCCAATAATGCGATAAATAACAAATGCC‑GGAACAT‑ACCCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCGGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAACCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                                                                                    <  2:379625/237‑1 (MQ=255)

                                                                                                                                   cGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccg                                                                                                                             >  2:797991/1‑229 (MQ=255)

                                                                                                                                   cGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGTTGc                                                                                                                   >  2:280949/1‑239 (MQ=255)

                                                                                                                                   cGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGc                                                                                                                   >  2:448477/1‑239 (MQ=255)

                                                                                                                                   cGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACAACGACCGAAGCGGTTACTGCAAAAACAACCGAGGACACCGCCgaggaaga                                                                                                                   >  1:1146954/1‑236 (MQ=255)

                                                                                                                                     agag‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAacca                                                                                                                                         >  2:505659/1‑215 (MQ=255)

                                                                                                                                     agag‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGCTGATGCa                                                                                                                  >  2:234969/1‑238 (MQ=255)

                                                                                                                                       ag‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAATCGGTTACTGCAATAACCACCAAAGGGACCGCCGATGATGAAAcc                                                                                                               >  2:720431/1‑239 (MQ=255)

                                                                                                                                       ag‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTTATTGCAATAAAAACCCAAGGCCCCCCCAAGAATGCAAca                                                                                                               >  1:100242/1‑238 (MQ=255)

                                                                                                                                       ag‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACGACGACCGAAGCGGTTACTGCAATAac                                                                                                                                           >  2:1122716/1‑211 (MQ=255)

                                                                                                                                        g‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGAACCGCCGATGATGCAACCa                                                                                                              >  2:551547/1‑239 (MQ=255)

                                                                                                                                         gaatggggcaaaaacaacccaaaaTCCCGCCAATAATGCGAAAAATAACAAATTCC‑GGAACAA‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAcc                                                                                                               <  2:789744/216‑1 (MQ=255)

                                                                                                                                             tGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGAATAAACAACCGCGGGCACCCCCGAGATTGCAACAAAtaa                                                                                                          >  1:917360/1‑237 (MQ=255)

                                                                                                                                                           cGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGATAAAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCa                                                                                              >  2:190357/1‑238 (MQ=255)

                                                                                                                                                            ccaaaacccGACAAAAAGGGGAAAAAAAAAAAATTCC‑GGGACAA‑AACCGCCAAGAT‑AACGAGGAACAGTGTTGTCCGGGTTT‑AT‑AGAGGAAAAA‑CAAAGTTCTGG‑CCA‑GG‑CAAAACCTACCC‑AAAAAAAAAAAAAA‑A‑GGACAGCAGCAATTTTAAGTAAACCACGACCACCGAAGCGGTTATTGAAAAACCCACCGAGGCACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGc                                                                                           <  1:1172038/232‑1 (MQ=255)

                                                                                                                                                             caaaacccccaaaaaatGCGAAAAATAACAAATCCC‑GGGACAT‑ACCCTGCCAGAT‑AAACAGGCACAGTTTTGTCCGGGTTT‑AG‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCa                                                                                          <  2:789078/225‑1 (MQ=255)

                                                                                                                                                              acaaccccacAATAATGGGAAAAAAAAAAAATTCC‑GGGACAA‑AACCAGACAGAA‑AAAACGGCACAGTGGTGTCCGGGTTT‑AA‑AGAGGTAAAA‑CCAAGTTCGGG‑CCA‑GG‑CAAAACCTACCA‑CAGAAAAAAAAAAC‑A‑GGACAGCAGAAATCTTAAGGGAACCACGACGACCGAAGCGGTTACTGCAAAAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                         <  1:1243231/230‑1 (MQ=255)

                                                                                                                                                              aactcccacaaaaaggtcaaaaaaaaaaaaaTCCC‑GGGAAAA‑AACCTGCCAAAA‑AAACGGGCAGTGTGTGGTCCGGGTTT‑AG‑AGAGG‑AAAA‑CCCAGTTCTGG‑GCA‑GGACAG‑ACCATCACACAGAAAAAAAAAAC‑G‑GGAAAGCGTCAATCTTAGGTGAACCCCGACGCCCGAAGCGGTTACGGAAATAACCCCCGAGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                         <  1:720431/218‑1 (MQ=255)

                                                                                                                                                                  aCCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAAc                                                                                      >  2:196185/1‑238 (MQ=255)

                                                                                                                                                                      ccAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGACAAACCCTaa                                                                                 >  2:577924/1‑239 (MQ=255)

                                                                                                                                                                      ccAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAACCACGACGACCGAAGAGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCCGGCCACGCAAAACCCTaa                                                                                 >  2:1256836/1‑239 (MQ=255)

                                                                                                                                                                      ccAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GA‑CAGAACCTCCAC‑CAAAAATAAAAAAC‑A‑GGACAGCAGCAATCTTCAGTGAATCACGACGACCGAAGCGGCTACTGCATTAACCACCGAGGGCACCCCCGATGCTGCAACCAATCACAACGCTGACCACGCCAAACCCTac                                                                                 >  2:862267/1‑238 (MQ=255)

                                                                                                                                                                         aaaatggaataaaaaaCAATTCCA‑GGGGCAT‑AACATGCCAGAA‑AAACGAGCACAGGGTGGTCCGACTTTCAA‑AGA‑GAAAAA‑CAAAGTCCTGA‑CCA‑GG‑AAGAATCTACAC‑CAAAAAGAAAAAAC‑A‑AGACAGCAGCAATCTTAAGTGAACCACGACGACCGAAGGGGTGACTGTAGTAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAg                                                                              <  1:1035054/231‑1 (MQ=255)

                                                                                                                                                                            acgcaaaaaataa‑AAATTCC‑CGGAAATAAACCTACCAAAA‑AAACGGGCAAATGGTGGCCGGGGTTT‑AA‑TGAGGAAAAC‑CCATGTTC‑GG‑CCA‑GGTCAAACCCTACAC‑CAAAAAAAAAAAAC‑A‑GGACAGAAGCAAGCTTAAGTGAACCCCGACGACCAAAGCGTTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGa                                                                           <  1:551547/232‑1 (MQ=255)

                                                                                                                                                                                 ataaataaCAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CCGCAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAAGCGGTTAATGCAATAACACCCCAGGGAACAGCCGATGATGCAACCAATCACACCCCTGGCCACCCAACACCCCAACAGGGATTTg                                                                       >  1:902110/1‑238 (MQ=255)

                                                                                                                                                                                 ataaataaCAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTg                                                                       >  2:926763/1‑238 (MQ=255)

                                                                                                                                                                                       aaCAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTACGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCACCCAATCAGAGCCCTGGCCACGCAAAACCCTAACAGGGAGTTg‑gacccgg                                                               >  2:1080555/1‑233 (MQ=255)

                                                                                                                                                                                        aaaaaTTCC‑GGGACAA‑AACCGCCCAGAA‑AACCGGGCAAGGTGTGGCCGGGGTTT‑AA‑AGAGGAAAAC‑CCAAGTTCTGG‑CAG‑GG‑AAGAGCCAACAC‑AAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTAAAGTGAACCACGACGACCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGa                                                              <  2:552579/237‑1 (MQ=255)

                                                                                                                                                                                            aTTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGAAGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCACTCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTc                                                           >  2:891073/1‑238 (MQ=255)

                                                                                                                                                                                              tCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACCACGACCGAAGCGGTTACTGCCATAACCACCGAGGGCACCGCCAATGATGCAACCAATCAAAACGCGGGCCCCCCAACACCCTAAAAGGGAGTTG‑GGAGCGGACTCACt                                                        >  1:676144/1‑239 (MQ=255)

                                                                                                                                                                                                          aaCCTGCCAGGT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAACGGGCTGGCAACCCAAAACCCAAAAAGGGAGTTg‑gagccggagtaaataaaagttagt                                              >  1:1152365/1‑216 (MQ=255)

                                                                                                                                                                                                               gCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGGGc                                         >  2:300731/1‑239 (MQ=255)

                                                                                                                                                                                                                        aacgggcaagtttgttgccGGG‑TT‑AT‑AGAGGGAAAACCCAGGTGCGGG‑CAA‑GG‑AAAAACCAAC‑C‑AAAAAAAAAAAAACAA‑GGAAACCAAAAAATTAAAGGGAACCACGACGCCCGAAGGGGTTCCTGCAATACCCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa                                 <  1:997188/222‑1 (MQ=255)

                                                                                                                                                                                                                                  ggtttttCCGGTTTT‑AT‑AAAGGAAAAC‑CCAGGTTC‑GG‑CCAGGG‑CGAAACCTACCC‑CA‑AAAAAAAAAAC‑AGGGACACCAGCAACCTAAAGTGAATCACGACGACCGAAGCGGTTCCGGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                       <  1:926763/233‑1 (MQ=255)

                                                                                                                                                                                                                                    tttttCCCGGTTT‑ATAAGAAGAAAAA‑CCAACTTCTGG‑CCG‑GG‑AAAAACTACCAC‑AAAAAAAAAAAAAC‑A‑GGACAGCAGAAACCTAAGGTGAACCCCGACGACCAAACGGTTTACAGCAATACCCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCATGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt                      <  1:508295/235‑1 (MQ=255)

                                                                                                                                                                                                                                    gttttacGGGTTT‑GT‑AAGGGTAAAA‑CCCAGTTCGGGCCCA‑GA‑CAGAACATAAAC‑AAGAAAAAAAAACA‑A‑GGACGGCCGAAATGTTAAGGGAATCACAACGACAGAAGCGATTACTGCAATACCCCCCGGGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt                      <  1:556009/233‑1 (MQ=255)

                                                                                                                                                                                                                                    gttgtCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTCCCGG‑CCA‑GG‑AAAAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACGCCGACCGAAGGTGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTg                         <  2:441508/235‑1 (MQ=255)

                                                                                                                                                                                                                                       gtCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                            >  2:747573/1‑118 (MQ=255)

                                                                                                                                                                                                                                       gtCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                            <  1:747573/118‑1 (MQ=255)

                                                                                                                                                                                                                                       gtCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGGTTAACTTAAGTTTTTGGGAGCAACAAAGACGGAATTAAGCGAc                   >  2:1107078/1‑238 (MQ=255)

                                                                                                                                                                                                                                        tCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGAATc                 >  2:565899/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGCCGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGAGGATACAACCAATAAACGCGCTGGCCACGCAAAAACCAACAAGGCAGTTGAGCCG‑GGGTTTACTACAGTTTATTGGGGCAACAAAAAAGGGAGTGAGGGACTCcaca             >  1:760276/1‑238 (MQ=255)

                                                                                                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGGATTGAGTGACTCCACAg            >  2:298958/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGAATTGAGTGACTCAACAg            >  2:869051/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCACCGGATTGCCTTAAGTTTTGTGGGGCAACAAGGACGGTATTGAGTGACTCAACAg            >  2:1217176/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGCAACCAATCAGACGGCTGGCCACGAAAAACCTTAACAGGGAGTTG‑GCAGCGGGTTCACTTAAGATTTGTGGAGCACAAAAAAAGGAATTGAGGGaaacaaaaa            >  1:374070/1‑231 (MQ=255)

                                                                                                                                                                                                                                               tt‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGt           >  2:879172/1‑238 (MQ=255)

                                                                                                                                                                                                                                                   t‑aGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTTGGGAGCAACAAAAACGGAATTGGGGGACTCACCAGAACCg       >  2:1120734/1‑239 (MQ=255)

                                                                                                                                                                                                                                                        gatAAAA‑CCAGGTTCTGG‑CCA‑GG‑CAGACCCACCAC‑CAAAAATAAAACAC‑A‑GGACAGCAACAATCTTAAGTGAATCACGACGCCCGAAGCGGTTCCTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGAtt                                                            <  2:665662/180‑1 (MQ=255)

                                                                                                                                                                                                                                                         gTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGGGCAACAAAGCCGGTATTGAGTGACTCAACGGTACCGGAAAt  >  2:345593/1‑239 (MQ=255)

                                                                                                                                                                                                                                                         gTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACGCAAAACCCTAACAGGGAGTTG‑GAAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAAt  >  2:1175581/1‑239 (MQ=255)

CGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTG‑GTT‑AAAAGAGA‑CCAGTTT‑CCCGCGAATATGA‑GCTGGAGCCA‑GTT‑CCGCAATATACATTGGCGAGAG‑CATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAAT  >  NC_000913/4241689‑4242163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`