BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,997	T→C	15.1%	D86G (GAT→GGT)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,997	0	T	C	15.1%	257.2 / 6.6	106	D86G (GAT→GGT)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (66/21); new base C (2/16); total (68/38)
Fisher's exact test for biased strand distribution p-value = 3.80e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTG  >  NC_000913/4241763‑4242233

aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCacg                                                                                                                                                                                                                                           >  2:1120889/1‑239 (MQ=255)

aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCacg                                                                                                                                                                                                                                           >  2:82982/1‑239 (MQ=255)

            cgAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGCGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑AGACAGCAGCAATATTAAGTGAATCacgaaacccgaaacg                                                                                                                                                                                                                               >  1:789078/1‑228 (MQ=255)

               atatgaatggtcgCCAGTTCCGCAATATACATGGGGG‑AGAGCATGGAGCCAAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGtt                                                                                                                                                                                                                            <  2:795552/227‑1 (MQ=255)

               atatGAGCTGGAGCCAGTTCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACGACGACCGAAg                                                                                                                                                                                                                                 >  2:93816/1‑234 (MQ=255)

                tgtgggttggaccagtttCAGCAAT‑TACATTGGGGTAGAGCATAG‑GGATAAACCAACCGCAAATCCCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                                                                                                                                                                                                           <  1:168986/223‑1 (MQ=255)

                taggAGCTGGAGCAAGTCCCGAATAATAAATTGGCG‑GGAGCATTGAGGTTAACCAAA‑CGCCAATCCCGCCAATAAGGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                                                                                                                                                                                                           <  2:703329/236‑1 (MQ=255)

                     gCTGGAGCCAGTTCCGAAATATACATTGGGG‑AGAGTATTGAGGCTAACCCAA‑CCCAAAAACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGc                                                                                                                                                                                                                       <  2:1216839/238‑1 (MQ=255)

                         gAGCCAGTTCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                                                                                                                                                                                                   >  2:763991/1‑238 (MQ=255)

                                ttCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTTACTGCAATAACCACCGa                                                                                                                                                                                                           >  2:435445/1‑239 (MQ=255)

                                ttCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGCTTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACATCAGCAACCTTAAGTGAACCACCATACCCGAAGCGGTTACTGCAATAAACACCgc                                                                                                                                                                                                           >  2:511262/1‑238 (MQ=255)

                                     cAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAACTAAAAAAA‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTATTGCAATAACCACCCAGGGc                                                                                                                                                                                                       >  2:556009/1‑238 (MQ=255)

                                      aaTATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGAAAGCAGCAATCTTAAGGTAATCACGACGACCGAAACGGTAACTGCAAAAacacacaagggga                                                                                                                                                                                                      >  1:739997/1‑227 (MQ=255)

                                        tataCATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGCTAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CACAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACCACGACCGAAGCGCTTACGCCCATAAACCCCAAGGGCAcc                                                                                                                                                                                                    >  1:761977/1‑238 (MQ=255)

                                          catgtatggggagaattttGAGAATAACCCAA‑CCACAACCCCACAAATAAGGCAAAAAATAA‑AAATTCCGGGGACAA‑AACCTGCCAGAT‑AAACGGGCAAGTTGTTGCCCGGGTTT‑AA‑AGAGAAAAAA‑CCAAGTTCCGG‑CCA‑GG‑CAGACCCTACCC‑CAAAAAAAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGCCGCCCGAGGCGGTTCCTGCAATACCCACCGAGGGCAccgc                                                                                                                                                                                                  <  1:1120889/222‑1 (MQ=255)

                                             aTTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAaccctgtatcttatacaaat                                                                                                                                                                                               >  2:1303474/1‑221 (MQ=255)

                                               tGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                                                                                                            >  2:1243231/1‑239 (MQ=255)

                                               tGGCG‑AAAGCATTGAGGCTAAACCAA‑CGCCAATACCGCAAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTCGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CACAACCTACAC‑CATAAATAAAAAAC‑A‑GGACAGCACCAATATTACGTGAATCACGACTACCGAACCGGTTACCGCCAAAACCACCGAGGGCAACCCCgaaga                                                                                                                                                                                            >  1:730262/1‑239 (MQ=255)

                                                  gg‑agaGTATTGCGCCAAAACCAC‑CGCAAACCCCGCCAATAATGCGATAAATAACAAATGCC‑GGAACAT‑ACCCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCGGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAACCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                                                                                                                                                          <  2:379625/237‑1 (MQ=255)

                                                  cG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccg                                                                                                                                                                                                   >  2:797991/1‑229 (MQ=255)

                                                  cG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGTTGc                                                                                                                                                                                         >  2:280949/1‑239 (MQ=255)

                                                  cG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGc                                                                                                                                                                                         >  2:448477/1‑239 (MQ=255)

                                                  cG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCCCCGATGATGc                                                                                                                                                                                         >  2:535218/1‑239 (MQ=255)

                                                  cG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACAACGACCGAAGCGGTTACTGCAAAAACAACCGAGGACACCGCCgaggaaga                                                                                                                                                                                         >  1:1146954/1‑236 (MQ=255)

                                                     agagCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAacca                                                                                                                                                                                                               >  2:505659/1‑215 (MQ=255)

                                                     agagCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGCTGATGCa                                                                                                                                                                                        >  2:234969/1‑238 (MQ=255)

                                                     agagCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGATT‑AT‑AGAGGTAAAA‑CCAAGTTCTGA‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATATTAAGTGAATCACGACTACAGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGACTATGCa                                                                                                                                                                                        >  2:789789/1‑238 (MQ=255)

                                                       agCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAATCGGTTACTGCAATAACCACCAAAGGGACCGCCGATGATGAAAcc                                                                                                                                                                                     >  2:720431/1‑239 (MQ=255)

                                                       agCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTTATTGCAATAAAAACCCAAGGCCCCCCCAAGAATGCAAca                                                                                                                                                                                     >  1:100242/1‑238 (MQ=255)

                                                       agCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACGACGACCGAAGCGGTTACTGCAATAac                                                                                                                                                                                                                 >  2:1122716/1‑211 (MQ=255)

                                                        gCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGAACCGCCGATGATGCAACCa                                                                                                                                                                                    >  2:551547/1‑239 (MQ=255)

                                                         gaatggggcaaaaacaacccaaaaTCCCGCCAATAATGCGAAAAATAACAAATTCC‑GGAACAA‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAcc                                                                                                                                                                                     <  2:789744/216‑1 (MQ=255)

                                                            tGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGAATAAACAACCGCGGGCACCCCCGAGATTGCAACAAAtaa                                                                                                                                                                                >  1:917360/1‑237 (MQ=255)

                                                                   aaaCCAA‑CGCCAATAACGGCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACATGCCAGAT‑AAACAGGCACAATGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCACTCTTCAGTGAATCCAGAAGACCGAAGCAGCAAACCGAATAACCACCGAGGGAACAGCAgaggaggaaaaacaacaaaacgaa                                                                                                                                                                         >  1:592140/1‑220 (MQ=255)

                                                                           cGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGATAAAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCa                                                                                                                                                                    >  2:190357/1‑238 (MQ=255)

                                                                            ccaaaacccGACAAAAAGGGGAAAAAAAAAAAATTCC‑GGGACAA‑AACCGCCAAGAT‑AACGAGGAACAGTGTTGTCCGGGTTT‑AT‑AGAGGAAAAA‑CAAAGTTCTGG‑CCA‑GG‑CAAAACCTACCC‑AAAAAAAAAAAAAA‑A‑GGACAGCAGCAATTTTAAGTAAACCACGACCACCGAAGCGGTTATTGAAAAACCCACCGAGGCACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGc                                                                                                                                                                 <  1:1172038/232‑1 (MQ=255)

                                                                             caaaacccccaaaaaatGCGAAAAATAACAAATCCC‑GGGACAT‑ACCCTGCCAGAT‑AAACAGGCACAGTTTTGTCCGGGTTT‑AG‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCa                                                                                                                                                                <  2:789078/225‑1 (MQ=255)

                                                                              acaaccccacAATAATGGGAAAAAAAAAAAATTCC‑GGGACAA‑AACCAGACAGAA‑AAAACGGCACAGTGGTGTCCGGGTTT‑AA‑AGAGGTAAAA‑CCAAGTTCGGG‑CCA‑GG‑CAAAACCTACCA‑CAGAAAAAAAAAAC‑A‑GGACAGCAGAAATCTTAAGGGAACCACGACGACCGAAGCGGTTACTGCAAAAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                               <  1:1243231/230‑1 (MQ=255)

                                                                              aactcccacaaaaaggtcaaaaaaaaaaaaaTCCC‑GGGAAAA‑AACCTGCCAAAA‑AAACGGGCAGTGTGTGGTCCGGGTTT‑AG‑AGAGG‑AAAA‑CCCAGTTCTGG‑GCA‑GGACAG‑ACCATCACACAGAAAAAAAAAAC‑G‑GGAAAGCGTCAATCTTAGGTGAACCCCGACGCCCGAAGCGGTTACGGAAATAACCCCCGAGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                               <  1:720431/218‑1 (MQ=255)

                                                                                  aCCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAAc                                                                                                                                                            >  2:196185/1‑238 (MQ=255)

                                                                                      ccAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGACAAACCCTaa                                                                                                                                                       >  2:577924/1‑239 (MQ=255)

                                                                                      ccAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAACCACGACGACCGAAGAGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCCGGCCACGCAAAACCCTaa                                                                                                                                                       >  2:1256836/1‑239 (MQ=255)

                                                                                      ccAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GA‑CAGAACCTCCAC‑CAAAAATAAAAAAC‑A‑GGACAGCAGCAATCTTCAGTGAATCACGACGACCGAAGCGGCTACTGCATTAACCACCGAGGGCACCCCCGATGCTGCAACCAATCACAACGCTGACCACGCCAAACCCTac                                                                                                                                                       >  2:862267/1‑238 (MQ=255)

                                                                                         aaaatggaataaaaaaCAATTCCA‑GGGGCAT‑AACATGCCAGAA‑AAACGAGCACAGGGTGGTCCGACTTTCAA‑AGA‑GAAAAA‑CAAAGTCCTGA‑CCA‑GG‑AAGAATCTACAC‑CAAAAAGAAAAAAC‑A‑AGACAGCAGCAATCTTAAGTGAACCACGACGACCGAAGGGGTGACTGTAGTAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAg                                                                                                                                                    <  1:1035054/231‑1 (MQ=255)

                                                                                            acgcaaaaaataa‑AAATTCC‑CGGAAATAAACCTACCAAAA‑AAACGGGCAAATGGTGGCCGGGGTTT‑AA‑TGAGGAAAAC‑CCATGTTC‑GG‑CCA‑GGTCAAACCCTACAC‑CAAAAAAAAAAAAC‑A‑GGACAGAAGCAAGCTTAAGTGAACCCCGACGACCAAAGCGTTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGa                                                                                                                                                 <  1:551547/232‑1 (MQ=255)

                                                                                                 ataaataaCAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CCGCAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAAGCGGTTAATGCAATAACACCCCAGGGAACAGCCGATGATGCAACCAATCACACCCCTGGCCACCCAACACCCCAACAGGGATTTg                                                                                                                                             >  1:902110/1‑238 (MQ=255)

                                                                                                 ataaataaCAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTg                                                                                                                                             >  2:926763/1‑238 (MQ=255)

                                                                                                       aaCAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTACGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCACCCAATCAGAGCCCTGGCCACGCAAAACCCTAACAGGGAGTTg‑gacccgg                                                                                                                                     >  2:1080555/1‑233 (MQ=255)

                                                                                                        aaaaaTTCC‑GGGACAA‑AACCGCCCAGAA‑AACCGGGCAAGGTGTGGCCGGGGTTT‑AA‑AGAGGAAAAC‑CCAAGTTCTGG‑CAG‑GG‑AAGAGCCAACAC‑AAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTAAAGTGAACCACGACGACCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGa                                                                                                                                    <  2:552579/237‑1 (MQ=255)

                                                                                                            aTTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGAAGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCACTCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTc                                                                                                                                 >  2:891073/1‑238 (MQ=255)

                                                                                                              tCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACCACGACCGAAGCGGTTACTGCCATAACCACCGAGGGCACCGCCAATGATGCAACCAATCAAAACGCGGGCCCCCCAACACCCTAAAAGGGAGTTG‑GGAGCGGACTCACt                                                                                                                              >  1:676144/1‑239 (MQ=255)

                                                                                                                          aaCCTGCCAGGT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAACGGGCTGGCAACCCAAAACCCAAAAAGGGAGTTg‑gagccggagtaaataaaagttagt                                                                                                                    >  1:1152365/1‑216 (MQ=255)

                                                                                                                               gCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGGGc                                                                                                               >  2:300731/1‑239 (MQ=255)

                                                                                                                                ccAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTACCTTAAGTTTTGTGGGGca                                                                                                              >  2:1143465/1‑239 (MQ=255)

                                                                                                                                 cAGTT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTAATGCAATAACCACCGAGGGCACCGCCGAGGATGCAACCAATCAGAGCGCGGGCCACGCAAAACCTCAACAGGGAGTGG‑GCAACGGGATCCCTTAAGATTTGTGGAGgaa                                                                                                             >  1:824555/1‑239 (MQ=255)

                                                                                                                                        aacgggcaagtttgttgccGGG‑TT‑AT‑AGAGGGAAAACCCAGGTGCGGG‑CAA‑GG‑AAAAACCAAC‑C‑AAAAAAAAAAAAACAA‑GGAAACCAAAAAATTAAAGGGAACCACGACGCCCGAAGGGGTTCCTGCAATACCCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa                                                                                                       <  1:997188/222‑1 (MQ=255)

                                                                                                                                                 aGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACa                                                                                                                                                     >  2:635963/1‑185 (MQ=255)

                                                                                                                                                  ggtttttCCGGTTTT‑AT‑AAAGGAAAAC‑CCAGGTTC‑GG‑CCAGGG‑CGAAACCTACCC‑CA‑AAAAAAAAAAC‑AGGGACACCAGCAACCTAAAGTGAATCACGACGACCGAAGCGGTTCCGGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                                                                                             <  1:926763/233‑1 (MQ=255)

                                                                                                                                                    tttttCCCGGTTT‑ATAAGAAGAAAAA‑CCAACTTCTGG‑CCG‑GG‑AAAAACTACCAC‑AAAAAAAAAAAAAC‑A‑GGACAGCAGAAACCTAAGGTGAACCCCGACGACCAAACGGTTTACAGCAATACCCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCATGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt                                                                                            <  1:508295/235‑1 (MQ=255)

                                                                                                                                                    gttttacGGGTTT‑GT‑AAGGGTAAAA‑CCCAGTTCGGGCCCA‑GA‑CAGAACATAAAC‑AAGAAAAAAAAACA‑A‑GGACGGCCGAAATGTTAAGGGAATCACAACGACAGAAGCGATTACTGCAATACCCCCCGGGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt                                                                                            <  1:556009/233‑1 (MQ=255)

                                                                                                                                                    gttgtCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTCCCGG‑CCA‑GG‑AAAAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGGGAATCACGCCGACCGAAGGTGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTg                                                                                               <  2:441508/235‑1 (MQ=255)

                                                                                                                                                       gtCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                                                                                                  >  2:747573/1‑118 (MQ=255)

                                                                                                                                                       gtCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                                                                                                  <  1:747573/118‑1 (MQ=255)

                                                                                                                                                        tCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGAATc                                                                                       >  2:565899/1‑239 (MQ=255)

                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGCCGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGAGGATACAACCAATAAACGCGCTGGCCACGCAAAAACCAACAAGGCAGTTGAGCCG‑GGGTTTACTACAGTTTATTGGGGCAACAAAAAAGGGAGTGAGGGACTCcaca                                                                                   >  1:760276/1‑238 (MQ=255)

                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGGATTGAGTGACTCCACAg                                                                                  >  2:298958/1‑239 (MQ=255)

                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGAATTGAGTGACTCAACAg                                                                                  >  2:869051/1‑239 (MQ=255)

                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCACCGGATTGCCTTAAGTTTTGTGGGGCAACAAGGACGGTATTGAGTGACTCAACAg                                                                                  >  2:1217176/1‑239 (MQ=255)

                                                                                                                                                             gTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGCAACCAATCAGACGGCTGGCCACGAAAAACCTTAACAGGGAGTTG‑GCAGCGGGTTCACTTAAGATTTGTGGAGCACAAAAAAAGGAATTGAGGGaaacaaaaa                                                                                  >  1:374070/1‑231 (MQ=255)

                                                                                                                                                               tt‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGt                                                                                 >  2:879172/1‑238 (MQ=255)

                                                                                                                                                                   t‑aGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTTGGGAGCAACAAAAACGGAATTGGGGGACTCACCAGAACCg                                                                             >  2:1120734/1‑239 (MQ=255)

                                                                                                                                                                        gatAAAA‑CCAGGTTCTGG‑CCA‑GG‑CAGACCCACCAC‑CAAAAATAAAACAC‑A‑GGACAGCAACAATCTTAAGTGAATCACGACGCCCGAAGCGGTTCCTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGAtt                                                                                                                                  <  2:665662/180‑1 (MQ=255)

                                                                                                                                                                         gTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGGGCAACAAAGCCGGTATTGAGTGACTCAACGGTACCGGAAAt                                                                        >  2:345593/1‑239 (MQ=255)

                                                                                                                                                                         gTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACGCAAAACCCTAACAGGGAGTTG‑GAAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAAt                                                                        >  2:1175581/1‑239 (MQ=255)

                                                                                                                                                                           aaaa‑CCAATTTCTGG‑CCA‑GG‑CAACAACTACAC‑AAGAAAAAAAAAAC‑A‑GGACAGGAGCAATCTAAAGGGAAACACGACGACCGAAGCGGTTACTGCAATAACCACAGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATaa                                                                      <  2:852519/239‑1 (MQ=255)

                                                                                                                                                                                  aaGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGAATTGAGTGACTCACCAGTACCGGAAATAAcggcgg                                                                >  2:311284/1‑239 (MQ=255)

                                                                                                                                                                                   aGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGGGACTCAACAGGACCGGAAATAAaggcggg                                                               >  2:879507/1‑238 (MQ=255)

                                                                                                                                                                                    gTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCCGAGCGCTGGCCACGCACAACCCTAACAGGGCGTTG‑GCCGCGGATTCCCTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGCCTCAACAGTCCCGGAAAAAAcgccgggc                                                              >  1:852519/1‑237 (MQ=255)

                                                                                                                                                                                    gTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGGTTCACTTAAGTTTTGTGGGGCAACAAAACCGGAATTGAGGGACTCAAAAGAACCGGAAAAAAaggggggg                                                              >  1:465864/1‑237 (MQ=255)

                                                                                                                                                                                    gTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGGGgtg                                                              >  2:167647/1‑239 (MQ=255)

                                                                                                                                                                                       ggcagggcctgcaaacactcacac‑AAAAAATAAAAACC‑A‑GGCCTGCAGAAACCGTAAGTGAACCACGAGTACCGAAGCGTTTCAGTCACTACCCCGCGATGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTTGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAAcggcg                                                                 <  1:180701/216‑1 (MQ=255)

                                                                                                                                                                                          g‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCAAGCAAAACCCTAACAGGGGGTTG‑GCAGCGGATTCACTTAAGTTTTTTGGAGCAACAAAGACGGAATGGAGTGACACAAAAGAACCGGAAAAAAcgaggggggaggat                                                        >  1:147230/1‑227 (MQ=255)

                                                                                                                                                                                                ccggaaaaacccaacaaaaaaaaaaaaaaC‑G‑GGACAGAAGAAACCTAAAGTGAACCCCGACGGACGAAGGGGTACCGGAAATCACCCCCGGAGACACCGCAGATGAGGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGcc                                                      <  1:879172/220‑1 (MQ=255)

                                                                                                                                                                                                  tcAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAACGCTGGCCACGCAAAACCCTACCAGGTGGTTG‑CCAGCGGATTCACTTAAGTTTATTGGCGCACCAAAGACAGTAATAAAGGACTCAAACTTACCGGAAAAAAcacagcagctggtacaaaa                                                   >  1:136696/2‑221 (MQ=255)

                                                                                                                                                                                                   caaaacccaacc‑cAGAAATAAAAAAC‑A‑GGACAGCAGGAAGCTTAAGGGAACCCCGACGCCCGGAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCa                                                     <  2:127552/225‑1 (MQ=255)

                                                                                                                                                                                                       aCCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTAACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGTGGTGGTTGCCCAataata                                              >  2:1135800/1‑239 (MQ=255)

                                                                                                                                                                                                        gtaaaagagccagaaaaaaaaC‑A‑GGACAGCAGCACTCTTAAGTGAATCACGACGCCCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAataata                                              <  2:324102/224‑1 (MQ=255)

                                                                                                                                                                                                         cTACCC‑CAAAAAAAAAAAAC‑A‑GGACAGCAGCAATCTAAAGTGAATCACGACACCCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                            <  2:917360/239‑1 (MQ=255)

                                                                                                                                                                                                                    aaTAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCACCAGTACCGGAAATAACGGCGGGTTCTTAGCCCAATGAAAAaacaccccatt                                  >  2:213561/1‑234 (MQ=255)

                                                                                                                                                                                                                     aTAAAAAAC‑G‑GGAAAGCAGAAATCTTAAGTGAGTCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGt                                 <  2:100242/239‑1 (MQ=255)

                                                                                                                                                                                                                     aTAAAAAAC‑A‑GGACAGCAGCAATCTAAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCCCCGAGGGCCACGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGt                                 <  2:676144/239‑1 (MQ=255)

                                                                                                                                                                                                                       taaaaaC‑AGGGACAGCGGCAATCTTAAGTGAATCACGACGACCGAAGGGGTTCCTGAAATAACCCCCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTa                                <  2:1302457/238‑1 (MQ=255)

                                                                                                                                                                                                                         aaaaC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGa                                                                                              >  2:641917/1‑174 (MQ=255)

                                                                                                                                                                                                                          aaaC‑A‑GGCAAGCAGCAATTGTAAGGGAACCGCGGCGACCGAAGGGTTTACGGAAATACCCAGCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGa                            <  2:166109/239‑1 (MQ=255)

                                                                                                                                                                                                                                       cagcaATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGGTGGCAAAAAATAAAACACCTAATGTAGCGGCCAAGGGaaac                 >  2:1161968/1‑237 (MQ=255)

                                                                                                                                                                                                                                            aTCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTTTCCCAAAAATAAAACACCCAATGGAGCGCCTCAAGTaagtgcgaa            >  2:667481/1‑232 (MQ=255)

                                                                                                                                                                                                                                              cTTAAGTGAATCACGACGACCGAAGGTGTTACTGCAATAACCACCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAata          <  2:147230/239‑1 (MQ=255)

                                                                                                                                                                                                                                                     gAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc    <  2:1146954/238‑1 (MQ=255)

                                                                                                                                                                                                                                                     gAACCACGACGACCAAAGGGTTTACCGCAAAAACCACCGAGGCCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc    <  2:1152365/238‑1 (MQ=255)

                                                                                                                                                                                                                                                      aaTCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGGGTGGTAGCCAAATAATAAAACACCCAATGTAACGCCTAAAGGAATCAAAAAAATAAAAcca  >  2:469519/1‑237 (MQ=255)

ACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCG‑AGAGCATTGAGGCTAAACCAA‑CGCCAATACCGCCAATAATGCGATAAATAACAAATTCC‑GGGACAT‑AACCTGCCAGAT‑AAACAGGCACAGTGTTGTCCGGGTTT‑AT‑AGAGGTAAAA‑CCAAGTTCTGG‑CCA‑GG‑CAGAACCTACAC‑CAGAAATAAAAAAC‑A‑GGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTG‑GCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTG  >  NC_000913/4241763‑4242233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`