BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,463,824	T→G	8.7%	D296A (GAC→GCC)	treC ←	trehalose‑6‑P hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,463,824	0	T	G	8.7%	349.2 / 2.0	141	D296A (GAC→GCC)	treC	trehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand): ref base T (51/71); new base G (9/7); total (63/78)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

GCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCG  >  NC_000913/4463586‑4464060

gcccgTTAACTGCGGGTTTGTCATGCCAATCTCTTCGCCCTGGTAGGTATTAGGCGTGCCCTGCATGCCATGCAGCCCCATCGCCAGCATTTTTGCCGCAGGCCCGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGt                                                                                                                                                                                                                                                  <  1:590391/236‑1 (MQ=255)

 cgcgTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGG‑ACAATGGTTTCAAC‑GCCACAAAGTc                                                                                                                                                                                                                                                 >  2:1128507/1‑239 (MQ=255)

 cgcgTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAAGGTTTTTAAC‑GCCCCAAAGTc                                                                                                                                                                                                                                                 >  2:240805/1‑239 (MQ=255)

  gcgTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCCTTCCTTGTTGCCAGTGGCGGA‑ACAATTTTTTCAAC‑CCCCCAAAGtac                                                                                                                                                                                                                                                >  2:713184/1‑237 (MQ=255)

      gAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCCCAAAATCCGGtt                                                                                                                                                                                                                                            >  2:1223029/1‑239 (MQ=255)

                        gCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCAttttt                                                                                                                                                                                                                          <  2:851114/239‑1 (MQ=255)

                          cAATCTCTTCCCCCTGGTAGTTTTCCCGCTTTCCCTGCATGCCCTTCGTGCCCATCGCCAGGATTTTTGCCGGAGGCACGCGGTATTCGCCTTCACCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTc                                                                                                                                                                                                                         <  1:183167/238‑1 (MQ=255)

                             tctcTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTTAAC‑CCCCCAAAAGCAGGTTTAACCAACGGCCCTTTTTcacc                                                                                                                                                                                                                      >  2:771174/1‑238 (MQ=255)

                             tctcTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAA‑GCCACAAAGGCAGGTTTAACCCGCGTCCCtttttttcc                                                                                                                                                                                                                      >  2:140739/1‑234 (MQ=255)

                                       cTGGTAGATTTACGGCGTTCCCTGCATGCCATGCAGCACCAGCGGCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAt                                                                                                                                                                                                           <  2:1126716/239‑1 (MQ=255)

                                       cTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAt                                                                                                                                                                                                           <  2:841172/239‑1 (MQ=255)

                                             gATTTACCGCGTTCCCTTGATTCCATGCAGCCCCATCGCCGACATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCt                                                                                                                                                                                                     <  1:827341/239‑1 (MQ=255)

                                                    ggCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑TCCACAAAAGCAGGGTTAGCCATCGTCCATTTTTTACCACCTGGACAATCGACCTT‑CAggag                                                                                                                                                                                             >  2:1156800/1‑237 (MQ=255)

                                                    ggCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCCCCACCGGGCTAATCGACCTT‑Cggggg                                                                                                                                                                                             >  2:1069814/1‑237 (MQ=255)

                                                        ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGCGAAACAATGCGCGGCTGATCGTGGTTCCACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGACGGACACAATTTTTTAAAC‑ACC‑CCAAGACAGCTTGTGCCACCAGCCACTTTTTACCACCCGGAAAAAACACCTT‑CCGGagaag‑a                                                                                                                                                                                        >  1:715135/1‑239 (MQ=255)

                                                        ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGG‑ACAATGTTTTCACC‑GCCACAAAGTCAGATTTAGCCAGCGTCCCTTTTTCTCCACCGGGAGaaactgacctcgcgggatga                                                                                                                                                                                          >  2:818179/1‑221 (MQ=255)

                                                        ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCCGGTTTAGCCAGCGTCCATTTTTTACCCCCCGGGAAATTGACCTT‑CCGGcgatg‑a                                                                                                                                                                                        >  2:1212928/1‑239 (MQ=255)

                                                        ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGGGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTCTTGCCAGTGGCGGA‑AACATGGTTTTAAT‑GCCCCAAAGTCAGGGTTAGACCGCGGCCCATTTTTACCCCCGGCGTAAAAGACCTTCCAGGtgtta                                                                                                                                                                                          >  2:784221/1‑236 (MQ=255)

                                                        gtCCATGCATGCCAGGCTGCACCATCGACAGCATTTTTTGCGCAGGCACGCGGTAGTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGGTCGTTGTTGCACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGtgatg                                                                                                                                                                                          <  2:473595/237‑1 (MQ=255)

                                                               catgccatgcCGCACCATCGACAGCATTTTTGCCGGAGGCCCGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATTCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTaa                                                                                                                                                                                 <  2:1307935/239‑1 (MQ=255)

                                                                       gcagcaCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGGCTCCATTTTTTACCCCCCGGCTAATCCACCCT‑CAAGGGTAG‑AAAATTAAAGGTCaa                                                                                                                                                                          >  2:1143156/1‑237 (MQ=255)

                                                                       gcagcaCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGGTGCCAGTGGCGGA‑ACCAGGTTTTCAAC‑GCCACCAAACCAGGGTTAACCAGCCTCCCATTTTAACCACCCGGCTCATCGCCCTT‑CAAGGGAGT‑GAAATTAAAAGTCATc                                                                                                                                                                         >  1:1077910/1‑239 (MQ=255)

                                                                        ccaacccATCGCCCGCATTTTTGCCGCAGGCCCCCGGTTATCCCCTTCAGCACCAAAGCTAGAAACAATGCGCGGCTGATCGGGGTTACACCAGAACAAGGCCTTCCATTCTACGTTGTGAATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCg                                                                                                                                                                        <  1:1277903/234‑1 (MQ=255)

                                                                         cgccccATCGCCAGCGTTGTTGGCGCAGGCACGCGGTATTCCCCTTCATCACCAAAGCGAGAAACAATGCGCTGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCCACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGa                                                                                                                                                                       <  1:217891/235‑1 (MQ=255)

                                                                           caCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTCCGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑CCCACAAAGTCAGGGTTATCCACCGGCCCCTTTTTACCACCCGGGTAAAAGCCCTT‑AAAGGGAAG‑AAAAATaaaagacaccaaca                                                                                                                                                                     >  1:737028/1‑228 (MQ=255)

                                                                              cATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGGTTAGCCAGCGGCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGGGATG‑GAAATTAAAGGTCATCgc                                                                                                                                                                       >  2:731419/1‑233 (MQ=255)

                                                                              cATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑AAAATGTTTTCAAC‑GCCACCAAGTCAGGTTTAGACGCCGCCCCTTTTTTCCACCCGGGGTAAACGCACTT‑CTGCGGCGG‑AAAAATAAAGAGAAACGACAAtt                                                                                                                                                                  >  1:469700/1‑239 (MQ=255)

                                                                                tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTTACCACCGGGATAATCGACCTT‑CAGGGGATG‑AAAATTAAAGGTCATCGCCAATTc                                                                                                                                                                 >  2:457425/1‑238 (MQ=255)

                                                                                tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAAAGTTTTCAAA‑GCCCCAAAATCAGGTTTTGCCCGGCTCCCTTTTTTACCACCCCGATAATCCACCCT‑CATGTGGTG‑GAAAATAAAAGGCATCGaaaaaata                                                                                                                                                                >  1:908586/1‑235 (MQ=255)

                                                                                tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑AAAATGTTTTTAAA‑GCCCCAAAGCACCGGTTAGCCTGCCTCCATTTTTTACCACCGCGGTAAAAGAACTT‑CAGGGGACGAAAAATTAAAgaacagcaaaaaaaa                                                                                                                                                                 >  1:925717/1‑225 (MQ=255)

                                                                                tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCAGCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCa                                                                                                                                                                <  2:56897/239‑1 (MQ=255)

                                                                                   ccaccctttttGCCCGCGGCCCGCGGTATTCACCTTCAACACCAAAGCCGAAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTAAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTg                                                                                                                                                             <  1:1128507/233‑1 (MQ=255)

                                                                                   ccAGGATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCAACAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTg                                                                                                                                                             <  2:595535/239‑1 (MQ=255)

                                                                                   ccAGCTTTTTTTCCGCAGGGCCGGGGTATTCACCTTCATCACCAAAGGGAGAAACAAGGCGCGGTTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTg                                                                                                                                                             <  1:457425/239‑1 (MQ=255)

                                                                                    ccggttttttGCGGCGGGCTCGGGGTATTACCCTTCAGCAACAAAGCCAGAAACAAGGCGCGGCTAATCGGGGTTGCACCGGAACAAGGCATTCCATGGTACGTTGTGCATTCCGTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGTTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTg                                                                                                                                                             <  1:457234/233‑1 (MQ=255)

                                                                                        aTTTTTTCCCGAGGCACGCGGTATTCCCCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTGGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGt                                                                                                                                                        <  2:432141/239‑1 (MQ=255)

                                                                                             tgtccggagcccccgtgaTTCACCTTTACCACAGAAGGGAAAACCAAGGCGCGGATGATCGTGGTTACACCAGAACAAGGCATTCCAGGCTACGTTGTGCATGCCTTGTTGCCAGTGGCGGA‑ACAATGTTGTCAAC‑GCCACAAAGTCAGGTTTAGCAAGGGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGAGG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCagag                                                                                                                                                   <  1:1072714/222‑1 (MQ=255)

                                                                                                             ttctccccTTCAACCCCAAGACGAAAAACAATGGCGGGCTGATGTTGGTTCCACCAGAACAAGGGATTCCATGCTACGTTGGGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTCCAAC‑GCCACAAAGTCAGGTTAAGCCAGCGTCCATTTTTCCCCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑Ccgcg                                                                                                                                             <  1:380139/222‑1 (MQ=255)

                                                                                                             tATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAt                                                                                                                                                                                                           <  2:459466/169‑1 (MQ=255)

                                                                                                              ttttcccTTCATCCCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGGTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCa                                                                                                                                  <  1:1069814/233‑1 (MQ=255)

                                                                                                              tttcacttttctccacAAATCGAGAAAACATGCGCGGCTTATCGTGGTTACACCAGAACAAGGCATTCCATGCTACATGGTGCATTCCTTGTTGACAGGGGCGGA‑ACAATGTTTTCAAA‑GTCACAAAGTCAGGTTTAGCCAGAGTCCATTTTTCACCTCCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCa                                                                                                                                  <  1:46085/223‑1 (MQ=255)

                                                                                                              aTTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCa                                                                                                                                  <  2:988651/238‑1 (MQ=255)

                                                                                                                   cctttacagcaaaaGCGAGAAAAAATGCGGGGCTGAGCGGGGTTACCCAAGAAAAAGGCATTCCCTGCTACGTTTTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTc                                                                                                                            <  1:1238370/228‑1 (MQ=255)

                                                                                                                       catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCCACACCCGGATAAACGGCCTT‑CAGGGTATG‑GAAATTAAAGGTCATCGACAATTTACTGCCTGTTAGAG‑CCGCGTATACCTGGAAATGGTccaa                                                                                                                         >  2:724101/1‑235 (MQ=255)

                                                                                                                       catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGCTG‑GAAATTAAAGGTCATCGACAATTCCCTGCCTGTTAGAG‑CCCGGTATCGCTGGccctgtctcctt                                                                                                                        >  2:380139/1‑228 (MQ=255)

                                                                                                                       catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAAGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGGCATCGACAATTAACTGCCTGTCAAAG‑cccccgatacccggcaaagcctcag                                                                                                                         >  2:71805/1‑215 (MQ=255)

                                                                                                                       catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGGCAGGTTTAGCCCGCGTCCATTTTTCACCACCGGGATAATCGCCCTT‑CCGGCGATG‑AAAATTAAAGGGCATCGACAATTAACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCCAg                                                                                                                         >  2:1238370/1‑238 (MQ=255)

                                                                                                                        atccCCAAAGCGATAAACAATGCGCGGCTGATCGTGGTTACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGc                                                                                                                       <  2:361011/239‑1 (MQ=255)

                                                                                                                           cccaaacgagaaaaCCATGGCCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCtgg                                                                                                                    <  2:1227164/228‑1 (MQ=255)

                                                                                                                              aaaGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGGCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCCCTGCCTTTCAGAA‑CCGCGGATCGCTGGGAATGTCCAAGGCtggggg                                                                                                                 >  2:468357/1‑239 (MQ=255)

                                                                                                                                agggagaACAAATGGGGGGGTGATCGTGGTTACACCAAAACAAGGCATTCCATGCTTCGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTggag                                                                                                               <  1:1318023/236‑1 (MQ=255)

                                                                                                                                aGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTggag                                                                                                               <  2:925717/239‑1 (MQ=255)

                                                                                                                                aGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGGGTTG‑AAAATTAAAGGTCCTCGACCATTCACTGCCTGTCACAG‑CCGCGTTTCGCTGGCAATGCCCAAGGCcggtgggg                                                                                                               >  2:426890/1‑237 (MQ=255)

                                                                                                                                    agaAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCCCAAAGTCAGGTTTTGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑TAGGGGATG‑AAAATTTAAGGTCATTGACAATTTACTGACTGTCAGAGACCGC‑TATTGCTGGCAAAGTCCAAAGCTGGGGGCGGACa                                                                                                           >  2:512429/1‑239 (MQ=255)

                                                                                                                                     gaAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGGCAAGTTTAGCCAACGTCCATTTTTCACCACCGGGGTAATCGACCTT‑CAGGAGATG‑AAAATTAAAGGTCATAGACAATTTCCTGCCTGTCATAG‑CCACGGATGGCTGGCAACGCTCCAGGCTGGTagaggcaca                                                                                                          >  2:130450/1‑235 (MQ=255)

                                                                                                                                     aaaaCAATGCGCGGGTTGTCGTGGTTACACCAGACCAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTGCAAC‑GCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACAt                                                                                                          <  1:771174/238‑1 (MQ=255)

                                                                                                                                      aaaCAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCGTTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACAt                                                                                                          <  2:255417/238‑1 (MQ=255)

                                                                                                                                       aaCCATTCGCGGCTTATCGTTGTTAACCCAGAACAAGGCATTCCATGCTACGTTGGGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACAtt‑t                                                                                                       <  2:536168/239‑1 (MQ=255)

                                                                                                                                       aaCAATGCGCGGCTGATCGTGGTTACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACAtt                                                                                                         <  2:1014359/238‑1 (MQ=255)

                                                                                                                                        ccAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑Tc                                                                                                      <  2:873108/238‑1 (MQ=255)

                                                                                                                                            tGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGGCATCGACAATTCACTGCCTGTCAAAG‑CCGCGGATTCCTGGCAATGCTCAAGCCTGGGGGAGGACCTT‑TTACCt                                                                                                  >  2:130611/1‑239 (MQ=255)

                                                                                                                                                           ggTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑AAAATGTTTTCAAC‑GCCACAAAGGCAGGGTTAGCCCGCGTCCATTTTTTTCCACCGGGATAAACGACCTT‑CAGGGGATG‑AAAATTAAAGGCCATCGACAAATCAATGCCCGTCAAAG‑CCCCGCATCGCTGGCACTGTTCAAGGCTGGTGGGGGGCATT‑TTCCCACCGGCAATTAAcccc                                                                                   >  1:36966/1‑238 (MQ=255)

                                                                                                                                                             ttACACCGGAACAAGGAATTCCATGATACGTTGTGCTTTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAG‑CGCACAATGTCAGGTATAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCcgc                                                                                  <  1:862020/238‑1 (MQ=255)

                                                                                                                                                             ttACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTTCCCACCGGGATAATAGACCTT‑CAGGGGATG‑AAAATTGAAGGGCATTGACAATTCACTGCCTGTCAGAG‑CCCCGTATCGCTGGCAAGTGTCAAGGCCGGGGGATGGCATT‑TTACCTACGGTCCTTAACCcgcg                                                                                 >  2:313421/1‑239 (MQ=255)

                                                                                                                                                              tACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGt                                                                                <  2:634140/239‑1 (MQ=255)

                                                                                                                                                                cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAAGTCATCGACAATTCACTGCCTGTCAGAG‑CCCCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGGCATT‑TCACCTACGGTCATTAACCCGCGTgg                                                                              >  2:287866/1‑239 (MQ=255)

                                                                                                                                                                cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAACCCGCGTCCATTTTTCACCACCGGGGTAATCGACCTT‑CAGGTGATG‑AAAATTCAAGGGCATCGACAATTCACTGCCTGTCACAG‑CCGCGTATCGCTGGCCATGCTCAAGGCTGGTGGCGGCCATT‑TCACCTACGGGCATTAACCCGCGTgg                                                                              >  2:958290/1‑239 (MQ=255)

                                                                                                                                                                 acCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTc                                                                                                                            <  2:970189/193‑1 (MQ=255)

                                                                                                                                                                 acCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTCAACACCGGGATAATCGACCTT‑CAGGGGATG‑AAAATTAAAGGTCATCCACAATTCACTGCCTGGCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGGGGGGGACATT‑TCACCTACGGGCCTTAACCcgctcggg                                                                             >  2:753098/1‑234 (MQ=255)

                                                                                                                                                                      aaCAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGGATCGCTGGCAATGTTCAAGGCTGGGGGGGGACATT‑TAACCTATGGTCCTTAAcctgtctcttataaa                                                                        >  2:465298/1‑226 (MQ=255)

                                                                                                                                                                      aaCAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACAACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAAAG‑CCGCGTATCCCTGGCAATGCTCAAGGCTGGGGGAGGAAATT‑TCACCTACGGTCATTAACCCGCGTGGCGTaaa                                                                        >  2:796350/1‑239 (MQ=255)

                                                                                                                                                                             ctttCCATGCGACGTTGTGCGTTTCCTTTTGCCAGTTGCTGA‑ACAATGTTTTCAAG‑GCCCCAAAGTCCGGTTGAGCCAGCGTCCATTTTCCACCCCCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCGCTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATc                                                                  <  1:750207/236‑1 (MQ=255)

                                                                                                                                                                             cATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGGCATCGACAATTCACTGCCTGTCAGAG‑CCCCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGGCATTAAACCCCAGGGGGGAAAcacaac                                                                  >  2:115540/1‑236 (MQ=255)

                                                                                                                                                                              ttttcctttctcGTTGTCCTTTTCTTTTTGGCGGGGGCGAA‑ATAATGTTTTCAAC‑GCCACAAAGTCAGGTGTAGCAAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATcgc                                                                <  1:130611/228‑1 (MQ=255)

                                                                                                                                                                                tCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGGGGACATT‑TTACCTACGGTCATTAACCCGCGTGGCCGAAAAACATCCCgg                                                              >  2:70670/1‑239 (MQ=255)

                                                                                                                                                                                     tcttcGTTGTTCATTCCTGGTTGCCAGTGGCTGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTTCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCAt                                                         <  1:792564/235‑1 (MQ=255)

                                                                                                                                                                                      cTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGGCATCGACAATTCACTGCCTGTCAAAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCCCGTGGCGTCAACACATCCCGGGTCAt                                                         >  2:212721/1‑238 (MQ=255)

                                                                                                                                                                                            tgtgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCCCAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CCGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGCCATTAACCCGCGTGGCGTAAACACATCCCGGGTCATCTCGTGc                                                  >  2:1084095/1‑239 (MQ=255)

                                                                                                                                                                                              tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCCCAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAAGTGATG‑AAAATTAAAGGTCATCGGCAATTCACTGCCTGTCAGAG‑CCGCGAATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGGAAAAAC‑‑CCCGGTTCCTTTCCTGCaaa                                               >  2:334256/1‑238 (MQ=255)

                                                                                                                                                                                              tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGGGTCCATTTTTCACCACCGGGATAAt                                                                                                                                                                                                           >  1:275276/1‑88 (MQ=255)

                                                                                                                                                                                              tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAt                                                                                                                                                                                                           <  2:275276/88‑1 (MQ=255)

                                                                                                                                                                                              tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAACGACCTT‑CACGTGATG‑AAAATTAAAATGCACCGACAATTCACTCCCTGTCACAG‑CCCGGTACCCCTTGCAATTCGCCAAGCTGGGGGAGGCAATTCTACCCCACGG‑CCTTAACCACCGGGGCGGAAAcacaaccccgggcactccgggcaa                                                >  1:498612/1‑219 (MQ=255)

                                                                                                                                                                                              tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGGTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGGTAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTTGCAGAG‑CCGCGTATCGCTGGCAATGCCCAAGGCTGGGGGAGGGCCTT‑TAAACAACGGCCATTAACCCCCGTGGCGTAAAAACCACCCGGTCCATCGTGTGcca                                                >  2:1029580/1‑237 (MQ=255)

                                                                                                                                                                                                       tgttgCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCgtg                                       <  2:729335/239‑1 (MQ=255)

                                                                                                                                                                                                       tgttgCCAGGGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCgtg                                       <  2:1095570/239‑1 (MQ=255)

                                                                                                                                                                                                       tggtgCCGGGGGCGGA‑AAAAT‑TTTTCAACAGCCACAAATGCAGGTTTGGACAGCGTACATTTTTCACCACCGGGATAAACGACATT‑CAGGTGATG‑AAAAGTAAAGGTCATCGACAAGTAACTGCCTGGCATAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCgtg                                       <  1:1223029/236‑1 (MQ=255)

                                                                                                                                                                                                        gttgCCAGTGGCGGA‑ACAATGTTTTCAAA‑GCCACAAAGTCAGGTTTAGCCAACGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGCATCGCTGGCCATGCTCAAACCTGGGGGAGGGCATT‑TCACCTCCGGGCATTCAACCGCGTGGCGTGAACCAACACCGGTTCATTTCGTggcacaacactgtg                                      >  2:1055173/1‑226 (MQ=255)

                                                                                                                                                                                                               gTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTAc                                                                                                >  2:301132/1‑174 (MQ=255)

                                                                                                                                                                                                                      a‑aCAATTGTTTAAAC‑GCCATGAAGTCAGGTTTAGCCAGCGTCCATTTTCCACCCCCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  2:842298/238‑1 (MQ=255)

                                                                                                                                                                                                                      a‑aCAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCCCCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  2:500110/238‑1 (MQ=255)

                                                                                                                                                                                                                      a‑aCAATGTTTTCAAC‑GCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTGCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  2:366506/238‑1 (MQ=255)

                                                                                                                                                                                                                      a‑aCAATGTTTTCAAC‑GCCAAAAAGGCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  2:585245/238‑1 (MQ=255)

                                                                                                                                                                                                                      a‑aCAATGTTGTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  2:740946/238‑1 (MQ=255)

                                                                                                                                                                                                                       atcattcttttCAAC‑TCCAAGAATGTAGGTTTAGCCAGCGGCAATTTTTCACCACCGGGATAATCGACCTT‑CAGGGGATG‑AAAAGTGAAGGTCATCGAAAATTCACTGCCTGGCAGAG‑CCCGGTATCGCTGGCAATGCTCAAGGCTGGTAGAGGACATT‑TCACCTACGGTCATTAACCCGCGTTGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  1:2673/231‑1 (MQ=255)

                                                                                                                                                                                                                       aaaaaTGTGTTAAAC‑GCCACAAATGGTGGTTTAGCCAGGGTCCATTTTTCACCACCGTGATAACCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  1:1143156/235‑1 (MQ=255)

                                                                                                                                                                                                                        aatattttttttaagcccACAAAGTAAGGTTTAGCCTGGTACCATTTTTAACCTCCGGGATAATCGACCTT‑CAGGTGATG‑AAAATGAAAGGTCAGCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                         <  1:70670/222‑1 (MQ=255)

                                                                                                                                                                                                                                 tCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCCCGGTTCATCTCGTGCAAAAACCCGGGTGCTCGGGGCCCATCGGTGTAGaac              >  1:840793/1‑238 (MQ=255)

                                                                                                                                                                                                                                       gCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCAATGCCTGTCAGAG‑CCGCGAATCGCTGGCAATGCTCACGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGGAAACACATCCCGGTTCATCTCGCGCAAAAACTCGCGTGCCCGTGGACCGTCGGGGAAGAAACGAc          >  1:969029/1‑238 (MQ=255)

                                                                                                                                                                                                                                            aaaGTCAGGTTTAGCCAACGTCCATTTTTCACCCCCGGGATAATAGCCCAT‑GAGGTGATG‑AAAATTAAAGGGCATCGACACTCCACTGCCTGGCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGt    <  1:511675/239‑1 (MQ=255)

                                                                                                                                                                                                                                              aGTCAGGTTTAGCCAGGGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg  <  2:847584/239‑1 (MQ=255)

GCGCGTGAAATGCGGGTTGGTCATGCCAATCTCTTCGCCCTGGTAGATATACGGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAC‑GCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTT‑CAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATGCTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCG  >  NC_000913/4463586‑4464060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`