BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	770,523	A→C	11.0%	H849P (CAC→CCC)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	770,523	0	A	C	11.0%	290.6 / 2.1	131	H849P (CAC→CCC)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand): ref base A (49/62); new base C (4/13); total (54/77)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TACAACAAAATCCCATGGGATGTGATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGC  >  NC_000913/770285‑770755

       aaaTCCCATGGGATGTGATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGCTGCGACTGTTGCTTTCA‑‑GTCGCGAGGGGATTTCTTGCTCAGAAACGCTGATGGATGAACACATTac                                                                                                                                                                                                                                      >  2:903229/1‑238 (MQ=255)

       aaaTCCCATGGGATGTGATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGGGATTTCTTTCTCAGAAACGATGATGGATGACACCATTac                                                                                                                                                                                                                                      >  2:1145144/1‑238 (MQ=255)

              aTGGGATGTGATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGGTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAgg                                                                                                                                                                                                                              <  2:801839/239‑1 (MQ=255)

               tGGGATGTGATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGGGATTTCTTGCTCAGAAACGATGATGGATGAacacctgtctcttataaa                                                                                                                                                                                                                             >  2:1150105/1‑225 (MQ=255)

                    tgtgATTAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGAATGTTGCTTTCA‑‑TTCGCGAGGTGATTTCTTGCCCAGAAACGATGATGTGGGAACAAATTAACACCGAGGaaaaaa                                                                                                                                                                                                                        >  1:836266/1‑237 (MQ=255)

                    tgtgATGAAGCTCAACAAAGCCGGATTCAAGGTGCCGGAACGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGACAGATGAAGTCCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCGGAAATAGCAACCTGTGTCGCGACTGTTGCTTTGAATATCGCGAGG‑GATTTC‑TGCTAAGAAAAGAAGATGGAATAAAAAATTTCCA‑CGCGGaaaaaaa                                                                                                                                                                                                                       >  1:423305/1‑236 (MQ=255)

                    tgtgATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCC‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATc                                                                                                                                                                                                                        >  2:1174880/1‑239 (MQ=255)

                    tgtgATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGAGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGCCTTGTGCTTTCA‑‑GTCGCACGGGGTTTTCTTGCTACGAAACGATGATGGTGTAACAAATTACCACCCAGGAAAAta                                                                                                                                                                                                                        >  1:484761/1‑238 (MQ=255)

                     gtgATGAAGGTAAAAAAAGCCGGATTCAACGTGACGGAAAGTTATAATTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATGTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGaaaagc                                                                                                                                                                                                                        <  1:325336/238‑3 (MQ=255)

                           aaGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACACCCGAGGAAAATCAAGGTTc                                                                                                                                                                                                                 >  2:484670/1‑239 (MQ=255)

                            aGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCCCTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTTTTGCTTTCA‑‑GTGCCGAGGGGAATTCTTGCTCAGAAACGATGTTGGATGACACCATTGACCCCGGGGAAAAACAAGGATCa                                                                                                                                                                                                                >  1:275013/1‑239 (MQ=255)

                            aGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTCGTTTTA‑‑GTTGCAGGGGGGTTTCTTGCTCAGAACAGAATATGGGGGAACACATACCCCCCCGGAAAAATAAAgggaaa                                                                                                                                                                                                                >  1:697114/1‑235 (MQ=255)

                            aGCCCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAGTCATCAACTCGGGATGGGCATCTGCCTTTCA‑‑ATACAGAGGAGATTTGTTGTTACGATACGAAGATGGATGGACGCATTtcaaccagaaaaaatacaaggtag                                                                                                                                                                                                                >  1:629175/1‑221 (MQ=255)

                                  acaaAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGGTTAATCCCGATTAATAAACAACCTGTGGACCGCCTGTTGCTTTCC‑‑ATCGCGGGGTGCATTCTTGCTCAGAAACGATGATGGATGAAACCATTACCAACAGGGAAAAGAAAGGGTCaaaagaa                                                                                                                                                                                                          >  1:1147541/1‑235 (MQ=255)

                                        ccGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGCGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCggg                                                                                                                                                                                                     >  2:966770/1‑238 (MQ=255)

                                         cGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCggg                                                                                                                                                                                                     <  2:1147541/237‑1 (MQ=255)

                                         cGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCggg                                                                                                                                                                                                     <  2:965687/237‑1 (MQ=255)

                                                       cGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGCCCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGc                                                                                                                                                                                     <  2:445721/239‑1 (MQ=255)

                                                               tgtgtttttttGAAAATGCCCCCAAGGGGAGGCAGAATAAGCGCACGTAAGAAAGTTGAAGACCGCAAAGAAGTGATTTTGCGGCTGTTTAATCCGGGTGAATCAGCAACCGGTGATGCGACGGTGGCTTTCA‑‑GTCGCGAGGTGATGTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAATTCAAGGTTCAAATCTATCGGGGCCTTTTTTCCCCGGCCAGTCa                                                                                                                                                                               <  1:1091938/232‑1 (MQ=255)

                                                                  gaccggtttAAAATGCCCCCAGGGGGATGCCTGAAAAGCGCACTTAAGAAAGCTGAAGAACGACAAGAATTGATTTTACGGCTGTTTAATGCGGCTGAATCAGCAAGCTGTGATGCGACTGTTGCTTTCA‑‑GTAGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGa                                                                                                                                                                           <  1:865576/233‑1 (MQ=255)

                                                                      tgttgAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTGTTGCTCAGAAACGATGCTGGATGAACACATTACCACCGGGGAAAATCAAGGTTCAAAACGATCGGGGCCTTTTTTACCCGGCCAGTCCCGGACGttg                                                                                                                                                                      >  2:1187661/1‑238 (MQ=255)

                                                                        ttgAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCAAGGACGTTAAg                                                                                                                                                                    >  2:1009879/1‑239 (MQ=255)

                                                                               tGCCCCCAGTGGGATGGCTGATAAGCGCAATTAAAAAAGCTGAAGGCCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCgg                                                                                                                                                             <  1:1280257/239‑1 (MQ=255)

                                                                                 cccccAGTGGGATGTCTTATAAGGCGACTTAAGAAAGCTGAAGCCCGACAAGAGGGGATTTTACGGCTGTTTAACCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGGTCAGTTACCgg                                                                                                                                                             <  1:757796/237‑1 (MQ=255)

                                                                                 cccccAGGGGCGTGCCTGATACGCGCACTTAAGAAAGCGAAAGCCCGAAAAGGAGTGATTTTACGGGTGTTTAATCCGGCTGAATCAGCAACCTGTGACGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTGCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑t                                                                                                                                                          <  1:354274/239‑1 (MQ=255)

                                                                                  ccccAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGCAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTa                                                                                                                                                                 >  2:865576/1‑232 (MQ=255)

                                                                                  ccccAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑tt                                                                                                                                                         >  2:757796/1‑239 (MQ=255)

                                                                                    cgaGTGGGATGCCTGAGAAGCCCAATTAAAAAAGTCGAAGTCCGACAAGAAGTGATTTTACGGCTTTTTAATCCGGCTGAATCAGCATCCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGc                                                                                                                                                       <  2:1088220/237‑1 (MQ=255)

                                                                                    ccAGTGGGTTGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCCGAAAGAAGTGATTTTACGGGTGTTCAAGCCGGAGGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACTCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGc                                                                                                                                                       <  1:279881/239‑1 (MQ=255)

                                                                                             tGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAGGGTTCAAATCTATCCGGGCCTTTTTTACCCGGCCAGTCACGGACGTCCAGTTACCGGC‑TTGCCTGAATAGc                                                                                                                                              >  2:605589/1‑239 (MQ=255)

                                                                                             tGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTCCCTGAATAGc                                                                                                                                              >  2:145328/1‑239 (MQ=255)

                                                                                             tGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGGGATTTCTTGCTCAGAAACGGTGATGGATTAACACGTTCACCCCGGGGGAAATCAAGGGTCAAATCTATTGGGGCCTTTTTTCACCGGCCGATCACGGAGCTTCAGTAACCGGC‑TTGcccgcaaaga                                                                                                                                              >  1:374879/1‑231 (MQ=255)

                                                                                                 tgtgaaGGGCACTTAAGAAAGATGAAGACCGACAAGGAGTGTTTTTACGGGTGTTTAAGCCGGGTGAATCAGCAACCTGGTATGCGACTGGTGGTTTCA‑‑GCCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAAt                                                                                                                                           <  1:703963/234‑1 (MQ=255)

                                                                                                  gATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTCATCCGGCTAAATCAGCAACCTGTGATGCGCTTGTTGCTTTCA‑‑ATCGCGAGTGGATTTCTTGCCCAGAAACGATGATGGATGAACAACTTACCACCGAGGGAAATCAAGGGTCAAATCTATCGGGGCCTGTTTTACCCGCCCAGTGCCGCACGTTACGTTCCCGGcggtcctgagaatcaatta                                                                                                                                          >  1:1140913/1‑221 (MQ=255)

                                                                                                  gATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGCCGTTCAGTTACCGGC‑TTGCCCGTATAGAAAtaa                                                                                                                                         >  1:1240146/1‑237 (MQ=255)

                                                                                                          cACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCC‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACAAATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTACAGGCCGTTCAGTTCACGGC‑TGGCCTTACTACACATCAAACCaaa                                                                                                                                  >  1:826918/1‑238 (MQ=255)

                                                                                                          cACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTCTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAAAGATGATGGATTAACACCTTACCACCGGGGAAAATCCAGGTTCAAATCTATCGAGGCCTTTTTTACCCGGCCCAGCACCGACGCTCCGTTCCCGGCTTTGCCTgtgagcaaacacaccaaa                                                                                                                                   >  1:722597/1‑221 (MQ=255)

                                                                                                              tAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGGTTTCTTGCTCAGAAACGATGATGGGTGAACACATTACCCCCGAGGCAAATCAAGGGTCAAATCTAACGGGGCCTTTTTTACCCGGCCAGGCACGGAAGTTACCGTACCGCC‑CTGCCTTGAAAGCAACCaaaccaaaaccaa                                                                                                                             >  1:1147611/1‑238 (MQ=255)

                                                                                                                     gCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGAAGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGCTGAACACATTCCCACCGAGGAAAATCAAGGTTCAAACCCATCGGGGCCTTTTTTACCCGGCCAGTTACGGACGGTCAGTTACCGGC‑TCGCCTGCATACCAATCAAACCCAAGACACAAAAgcg                                                                                                                      >  1:1053862/1‑239 (MQ=255)

                                                                                                                       tGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCgg                                                                                                                                                             >  2:465019/1‑199 (MQ=255)

                                                                                                                       tGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCgg                                                                                                                                                             <  1:465019/199‑1 (MQ=255)

                                                                                                                       tGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATgcgc                                                                                                                     >  2:263460/1‑238 (MQ=255)

                                                                                                                       tGAAGACCGAAAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATgcg                                                                                                                      <  2:697114/237‑1 (MQ=255)

                                                                                                                          aGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGcc                                                                                                                 <  2:826918/239‑1 (MQ=255)

                                                                                                                          aGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGcc                                                                                                                 <  2:220424/239‑1 (MQ=255)

                                                                                                                              cGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGGGACTGTTGCTTTCA‑‑GTCGCGGGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTAAAATCCATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCATATAACCGC‑TTGCCTTAACTGCCATCAAACCGCAGCCACatttaccgcgcccaat                                                                                                             >  1:1037279/1‑225 (MQ=255)

                                                                                                                                aCAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGCCTGTTGCTTTCA‑‑GTCGCGAGGGGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACAACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAAAAGCAATCAAAACGAAGCCACATATGCACGGCCAGAttg                                                                                                           >  2:340797/1‑239 (MQ=255)

                                                                                                                                aCAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTAACGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCCGCCAGAttg                                                                                                           >  2:541562/1‑239 (MQ=255)

                                                                                                                                   agaagTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGAttgttg                                                                                                        >  2:5571/1‑239 (MQ=255)

                                                                                                                                         atgtttCCGGTTGTTTAATCCGGCGGAATCAGAAACCTGTAATGCCACTTTTCCTTTCA‑‑GACGGGAAGTTATTTCTTGCTCAGGAACGATGATGGATGAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTAAC‑GGGCCCTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACaaa                                                                                                   <  2:911926/234‑1 (MQ=255)

                                                                                                                                           ttttACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAgg                                                                                                 <  2:484761/238‑1 (MQ=255)

                                                                                                                                           ttttACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAgg                                                                                                 <  2:271574/238‑1 (MQ=255)

                                                                                                                                           ttttACGGCTGTTTAATCCGGCTGAAGCAGCAACCTGGGAGGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAgg                                                                                                 <  2:338104/238‑1 (MQ=255)

                                                                                                                                              agacagtGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGgcgc                                                                                             <  2:535367/233‑1 (MQ=255)

                                                                                                                                               cctgttGTTTGATACGGATGAATCGAGAACCTGTGATGCGACTTTTGTGTGCC‑‑AGCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTCCCACCGAGGAAAATCAAGGTTCAAAGCTATCGCGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCt                                                                                            <  1:980575/234‑1 (MQ=255)

                                                                                                                                                      tttAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCa                                                                                     >  2:729452/1‑239 (MQ=255)

                                                                                                                                                      tttAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTGTACAAAGGGCGCTTTTTTCa                                                                                     >  2:355856/1‑239 (MQ=255)

                                                                                                                                                      tttAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATGTTTGACAAAGGGCGCTTTTTTCa                                                                                     >  2:34070/1‑239 (MQ=255)

                                                                                                                                                       ttAATCCGGCGGAAACGGGAACCTGTGATGCGACTTGTGCTTTCA‑‑GGCGCGAGGTGATTTCTTGCTTAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCAt                                                                                    <  2:853871/239‑1 (MQ=255)

                                                                                                                                                        tAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATg                                                                                   <  2:1240146/239‑1 (MQ=255)

                                                                                                                                                        tAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATg                                                                                   <  2:1049447/239‑1 (MQ=255)

                                                                                                                                                         aaTCCGGCTTAAGCAGCAACCTGTGGTGGCACTGTTGCTTTCA‑‑GGCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACGCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTGTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCTGATTGTTGACAAAGGGCGCTTTGTTCATGc                                                                                  <  2:973274/239‑1 (MQ=255)

                                                                                                                                                               gCTGAATCAGCAACCTGTGATGCGATTGTTTCTTTCA‑‑GGCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATa                                                                            <  1:729452/239‑1 (MQ=255)

                                                                                                                                                               gCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAACCCGAACCCCCCTATGCGCGGCCCAATTGTTTGCAAAGGGGCCTTTGTTCaatccagtc                                                                             >  1:535367/1‑230 (MQ=255)

                                                                                                                                                                cTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTAc                                                                                                                                                                <  2:914997/155‑1 (MQ=255)

                                                                                                                                                                cTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTAc                                                                                                                                                                >  1:914997/1‑155 (MQ=255)

                                                                                                                                                                       cgcgactcggtatggagcTGTTGTTTTCG‑‑TGCGCGAGGTAGTTTTTTGCTCAAAAACGATTATGGAAGAACACATTACCACCGAGGAAAATCGATGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑a                                                                   <  1:903229/222‑1 (MQ=255)

                                                                                                                                                                          aatcTGTGATGGCACTGTTGCTTTCA‑‑GTCGCGAGGTTATTTCTTTCTCAAAAACGATGATGGATGAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑a                                                                   <  2:1297341/233‑1 (MQ=255)

                                                                                                                                                                                 taTGGGACTTTTTCTTTCA‑‑GTCGCGAGGTGATTTCTTGGTCAGAAACGATGATGGATGAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTAtt                                                         <  1:145328/238‑1 (MQ=255)

                                                                                                                                                                                 taTGCGCCTGTTGCTTTCA‑‑GTCGGGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTAtt                                                         <  2:176446/238‑1 (MQ=255)

                                                                                                                                                                                 gttggggtttgttgtttCA‑‑GTCGCGGGGTGATTTCTTGCTCAGAAACGATGATGGATGACCACTTTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTAtt                                                         <  1:1009879/225‑1 (MQ=255)

                                                                                                                                                                                                   a‑‑GTTGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTAACGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  2:836266/239‑1 (MQ=255)

                                                                                                                                                                                                   a‑‑GTCGCGAGGTGATTTATTGCTCAGAAACGATGATGGATGAACACATTCCCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  2:121448/239‑1 (MQ=255)

                                                                                                                                                                                                     ggtccggagggtaTTTATTGATCAGAAACGATGGTGGAGGAACACATTACCACGGAGGAAAATCAAGGTTAAAATCTTTAGGTGCCTTTTTTTCCCGGCCAGTCACGGACGTTTAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  1:965886/227‑1 (MQ=255)

                                                                                                                                                                                                     agttgtgtgttgttttCTGGCTCAGAAACGATTATGGATGAACACTTTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  1:202356/226‑1 (MQ=255)

                                                                                                                                                                                                     aggcgcgATGTGATTTCATGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  2:422576/236‑1 (MQ=255)

                                                                                                                                                                                                     aatcggggggTTATTTCTTTCTCAGAAACGATGGTGGGTGAACCTATTACCACCGGGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  1:484670/231‑1 (MQ=255)

                                                                                                                                                                                                       tCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCCAACCGAAGCCACATATTCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCCTGAAACACTTT‑TTCGGGCTCAAAAAAAAGGCaa                                     >  1:903684/1‑239 (MQ=255)

                                                                                                                                                                                                       tCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTAAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAATCAGGGaa                                     >  2:831571/1‑239 (MQ=255)

                                                                                                                                                                                                        cgcgATGTGATTTCTTGCTCAGAAACGATGATGGATTAACACATTACCATCGAGGAAAAGCAAGGTTCAAAGCTATCGGTGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  2:169144/236‑1 (MQ=255)

                                                                                                                                                                                                        cgcgAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  2:1075670/236‑1 (MQ=255)

                                                                                                                                                                                                        cgcgAGGTGATTGCGTGCTCAGAAACGATGATGGATGAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGc                                       <  2:1025821/236‑1 (MQ=255)

                                                                                                                                                                                                          cgAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGATCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTTAATAGCAATCAAACCGAAGCCACATCTTCCCGGCCCAATTGTTGCCAAAGGGCGCTTTTGTCATGCCGGTTACGGCCTA‑GAAGCTTTACTCGGTCTACcaaaacgggacaata                                  >  1:1025821/1‑228 (MQ=255)

                                                                                                                                                                                                             ggTGATTTCTTGCTCAGAAACGATGATGGATGAACCCATTCCCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAAtt                                  <  2:445804/236‑1 (MQ=255)

                                                                                                                                                                                                                taTTTCTTGGCAAAAAAGGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATTGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAata                             <  2:125321/237‑1 (MQ=255)

                                                                                                                                                                                                                gATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAata                             <  2:841299/238‑1 (MQ=255)

                                                                                                                                                                                                                gATTTATTGCTCAGAAACGATGAGTGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAAGCTATCGGGGCCTGTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAata                             <  2:1140913/238‑1 (MQ=255)

                                                                                                                                                                                                                caattCTTGCTCAGAAACGATGATGGATGAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGTGCGTTCAGTTACCGTC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAata                             <  2:232559/235‑1 (MQ=255)

                                                                                                                                                                                                                 tttttttTCTCAGAAACGATGATGGATTAACACTTTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAatata                           <  1:496781/238‑1 (MQ=255)

                                                                                                                                                                                                                 aTTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCCCATATGCGCGGCCAGATTGTTGGCAAAAGGGCCTTTGTTCATGCCGGATACGGCCTC‑AACGCTTTTTTCGGTcgcaaaaaaaaagcaaaacaaaaa                             >  1:1093505/1‑214 (MQ=255)

                                                                                                                                                                                                                     cTTGCTCAGAAACGATGATGGATGAACACATTacca                                                                                                                                                                                                                                    >  2:161348/1‑36 (MQ=255)

                                                                                                                                                                                                                     cTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCCTTAATCGGTCTACAAAAGCAGGCAAATTCCATATAATGc                       >  2:255824/1‑239 (MQ=255)

                                                                                                                                                                                                                       ttctcagtaacgagttaggatgAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAATTGCCGGC‑TTGCCGTAATATCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCag                     <  1:325500/222‑1 (MQ=255)

                                                                                                                                                                                                                       ttcgcaaaaaCGTGGATTGATGAACACATTACCACCGAGGAAAAGCAAGTTTCAAAGCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCag                     <  2:1091904/232‑1 (MQ=255)

                                                                                                                                                                                                                       tGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCag                     <  2:374879/239‑1 (MQ=255)

                                                                                                                                                                                                                                     tttggatgAACCCATTTCCCCCGGGGAAAATCCAGGTTCAAATCTATCGGGGGCTTTTTTACCCGGCCAGTCACGGACGGTCAGTTACCGGA‑TTGCCGGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGaca       <  1:1101519/237‑1 (MQ=255)

                                                                                                                                                                                                                                          aagAACCCATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCagag                   <  2:200792/220‑1 (MQ=255)

                                                                                                                                                                                                                                           tgAACCCCTTACCACCGAGGAAAACCAAGGTTAAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAACCAATCCGAAGCCACATATGCGCGGCCAGATTGGTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGc  <  2:365866/238‑1 (MQ=255)

                                                                                                                                                                                                                                           tgAACACATTACCACCGAGGAAAATCAAGGTTCAAATCCATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGc  <  2:701833/238‑1 (MQ=255)

                                                                                                                                                                                                                                           gtaaCACATTCCCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGc  <  2:1312171/236‑1 (MQ=255)

TACAACAAAATCCCATGGGATGTGATGAAGCTCAACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGATGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCA‑‑GTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGC‑TTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATG‑AACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGC  >  NC_000913/770285‑770755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`