| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 134,775 | T→C | 19.4% | intergenic (+25/+13) | yacL → / ← speD | UPF0231 family protein/S‑adenosylmethionine decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 134,775 | 0 | T | C | 19.4% | 102.8 / 5.1 | 73 | intergenic (+25/+13) | yacL/speD | UPF0231 family protein/S‑adenosylmethionine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base T (36/19); new base C (2/14); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCA > NC_000913/134550‑134996 | tCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTTGCGGCTTACCGCAATTTCGTGCAGCAGAAGGAAATAAATTTGGCGGAGC‑CGGGGGGCTCCg > 1:720133/1‑229 (MQ=255)tCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGGGCTCCg > 1:153145/1‑229 (MQ=255)tCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCATGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCCATTTGTTGCACAGGAAGTAAAAAAATTTTGCGGGGC‑CTGGGGGCTCCg > 2:108222/1‑229 (MQ=255) gcacggggcaggggaGGAATCCCCCCTGTGGATGGTGGGGGAAGAGGTGATGGTTCGGTCAAATAAACTGAAATTCGCGGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGGGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCag < 2:79894/223‑1 (MQ=255) gggcagggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CGGGGGGCTCCGCCAGAGCCGt > 1:446867/1‑229 (MQ=255) gggcagggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCCCATTTTGGTGCAGCAGAGGGAAAAAAACTTGGGGGGGC‑CTGGGGGTCCCGcccagaccct > 2:364882/1‑221 (MQ=255) gggcaTGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTAAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCTGGCAGCAGAAGCAAAAAAATCGTGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTacaa > 1:693839/1‑226 (MQ=255) gAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCCCCAGAGCCGTTAAACAGCTg > 1:367664/1‑228 (MQ=255) cacCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGTCGGAGC‑CTGGGAGCTCCCCCAGAGCCGTTAAACAGCTGGCaaat > 1:392551/1‑229 (MQ=255) acCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGATCCGCCAGAGCCGTTAAACAGCTGGCCTAtt > 1:647617/1‑229 (MQ=255) cccTGTGGATGGCCGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGACGAAGTAAATAAATCGGGCGGAGC‑CTGGGAGCTCCGCCAAGGCCGTTAAAAACATGGCAAAtta > 2:281126/1‑228 (MQ=255) tgtgGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGGGCCGTTAAACAGCTGGCATATTgcgc > 1:234421/1‑229 (MQ=255) gtgGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCCGAGC‑CTGGGGGCTCCGCCCGAGCCGTTAAACAGCTGGCCAATTtcgc > 1:643183/1‑228 (MQ=255) tggatggaCGGGGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGTCGGAGC‑CTGGGGGGTCCGCCAGAGCCGTTAAAAATCGGGCATATTGCGcc > 1:267104/1‑228 (MQ=255) ggggtggaCGGGGAAGGGGTGGTGTGTCGCGCCAATCAACTGAAATGCGGTGGCGATGAAATGGAAGAGGGTATGAACTAATCCGACGAAGAAAGCCTTGCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGcc < 1:376387/224‑1 (MQ=255) gggatgggCGGTGAAGGGGTGGTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGGGGGGATTAACTACTACGACGAAGAAAGCCTGTCGCTAGGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgcgc < 1:364882/226‑1 (MQ=255) gTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTAACGCAATTTCGTGCAGCCGAAGTAAATAAATCTGGCGGGGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCGGGCACATTGGGCCCGTAATAAAtc > 1:640269/1‑229 (MQ=255) gTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGAGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCATAAGTAAATAAATCTGGGGGAGC‑CTGGGGCCCCCGCCAGAGCCCTAAAACACATGGCAATCTGGGCCCGtaactaaca > 2:399512/1‑223 (MQ=255) ggggtGGTTCGCGCCAATCAACTGGAATTCGGTGGCGATGAAATGGAAGAGGGGATGACCTCCTACGACGAAGAAAGCCTGTCGCTAGGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCAAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCa < 1:544859/224‑1 (MQ=255) ggggtGGGTCGCACCAATCACGGGGAAGTCGGTGGCGATGAAATGGAAGAGGGGAGGAACTACTACGACGAAAAAGGCCTGTCGGTATGGGGTGTTGAGGATTTTAT‑GA‑AG‑GTCGTGGGGGCTTACCGCAATTTCGTGCAGCAGAATTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCa < 2:153145/224‑1 (MQ=255) aTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCCGAGCCGTTAAAAAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAttt > 1:384224/1‑228 (MQ=255) aTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAAACGCTGGAATATTGCGCCCGGAATAAATCTCGCGCAatt > 1:150945/1‑228 (MQ=255) aTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGCCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTCGGCCCGTAATAAATCTCGCGCAttt > 1:416039/1‑228 (MQ=255) aTGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGGAAACAAATCTGGCGGAGC‑CCGGGCGCTCCGCCAGAGCCGTTAAAAAACTGGCCTATTTGCACCGTAATAAACCTCCCGCCtttt > 2:745622/1‑228 (MQ=255) cgcgCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAGGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGCGCCGTTAAACACCGGGCATATTCCGCCCGTACTAAATCTCGCGCATTTCTTTCca > 1:380034/1‑229 (MQ=255) cgcgCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCATAGCCGTTAAACAGCTGGCATATTGCGCCCGGAATAAATCTCGCGCATTTCTTTccc > 1:532121/1‑228 (MQ=255) gcaaagcAACGGGAATTGGCTGGCAGGGAAATGGAAGAGGGGATGAACTACGACGACGAAGAAAGCCTGTCCTTATGCGGCGTTGAGGATTTTCG‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTGCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCAcag < 2:522622/223‑1 (MQ=255) cgggCGGTGAAAGGGAAGGGGGGATGAACTCCTACGACGAAGAAAGCCTGGCGCTATGCGGCGTTGGGGATTTTCT‑GC‑AG‑GGCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTcgcg < 2:485839/193‑1 (MQ=255) cTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTccctgcctcttttacacacctccg > 1:485839/1‑206 (MQ=255) cTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTGATTTCCTgg > 1:657344/1‑229 (MQ=255) ggggggggATGAATTCCTAGGACAAAGAAAGCTTGTCGCTATGCGGCTGTGAGGATGTTCG‑GG‑AG‑GTCGGGGCGGCTTCCCGAAATTCCGTGCAGCAGAAGTAAATAAATCGGGCGGAGC‑CGGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGtt < 2:450247/225‑1 (MQ=255) tcttacGAGCAAGAAAGCCTGCCCCTATGGGGCTTTGGGGATTTTCG‑GC‑GG‑GTCGTGGCGGTTTCCCGCATTTTCGGGCAGAAGAAGAAAATAAAGCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGtt < 2:367664/225‑1 (MQ=255) actacGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTCATTTCCTGGCGCTCGCTGTCGGTTAGGTCTTCCGGtt > 1:600107/1‑227 (MQ=255) actacGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCGCGCTGCCGGTTAAGTCCTCCGGGTTg > 2:321633/1‑229 (MQ=255) ctacGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCATGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATAATGCGCTCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCACCGGGAATATCCTGGCGCTCCCTGTCGGTTAAGGCTTCCCGTTTgg > 1:683673/1‑229 (MQ=255) tctgtcgaAGAAGGTCTGTTCCTATGCGGGGTTGAG‑‑TTTTTT‑GCAAGAGTGGTGGCGTCTCCGCGAATTTTGGTCCATAAGACGTAATTACTGCGGGCGGAGC‑TTGGGATCTCCGCATGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAGTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTGGCTGTCGGTTAAGTATTCCGGTTTGgt < 2:131971/224‑1 (MQ=255) acgagaaagaaagctggtgcttATGTGGCGATGAGGATTTTCT‑GC‑GG‑GTGTGGGCTGCTTACGGCATGTTCGAGAAGCAGAAGTAAATAAGTCTGGCGTGCC‑CTGGGAGCACGCCCAGAGCCGTTAAACAGCTGTCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgt < 2:108958/207‑1 (MQ=255) acgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCCCAGCCCAGGGGGAATTTCCTTGGGCCCGCCGTCGGTTAAGTCTTCCCGGTTggggggg > 2:666221/1‑224 (MQ=255) gaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGGAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACAt > 1:423893/1‑229 (MQ=255) gaggctctctgccttGCGGCGTTGAGTATTTTCG‑GC‑GG‑GTGGTGGCGGCTTACCGCAATTTCGTGCAGCCGAAGTAAAAAAATCGGGGGGCGC‑CTGGGACCTCCGCACGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATg < 2:657343/215‑1 (MQ=255) agcttttggCTAGGGGGCGTGGAGGTTTTGTG‑GC‑GG‑GTCGTGGCGGCTTCCCGAAATTTCGGGCAGCGGAAGTAAATAAACCGGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTa < 2:392551/221‑1 (MQ=255) gTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCCCAGCGCAGCGGTAAGTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGGGTGGagacggtagggc > 1:603190/1‑217 (MQ=255) gTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGGCTTCCGGTTTGGGGTGGAACATGTAGGGc > 1:195196/1‑228 (MQ=255) cctttcgggggttttgagtttttGT‑GG‑AG‑GTTGTGGGCGCTGTCCGCCTCTTCGTGAAGCGGAAATAAAAAAGTCTGGCGGAGC‑CTGGGCGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTa < 2:347462/210‑1 (MQ=255) tggcgTTTGGGATTTTGC‑GC‑AG‑GGCGTGGCGGGTTGCCGCAATTTCGTGCAGCAAGAGAAAAAAAATCCGGGGGGGC‑CTGGGACCCGCGCCAGGGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa < 2:301029/228‑1 (MQ=255) gggctTTGAGGTTTTTTT‑GC‑AG‑GTCGGGGCGGGTTCCCGGCATTGAGTCAAGGCATAGAAAATAAATCGGGCGGGGC‑TGGGAAGATCCGCCGGAGGCGGTAAACAGCTGGCATATTACGCCCGTAATAAATCTCGCGCAGTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa < 2:635774/228‑1 (MQ=255) cggtttgggtgtgtttCT‑GC‑AG‑GGCCGGGGAGTTTCCCGAAATTCCGGGAAGCAGAATTAACAAAATGTGGTGGAGC‑ATGGGAGCCCGGCAGGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAAGCTCGCGCATGTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTGTGGTGTGGAACATGTAGTGCTTAAGGTCGa < 2:401209/216‑1 (MQ=255) cggcgTTGA‑GATTTTCTGGA‑AG‑GTCGTGGCGGCTTACCGGAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGACCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGa < 1:666222/229‑1 (MQ=255) ggcgTTGAGGATTTTCT‑GC‑AG‑GTCGTGGGGGCTTACCGCAATTTCGTGCAGCAAAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGaa < 1:496109/229‑1 (MQ=255) cgTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGGTAAGTCTTCCGGTTTGGTGTGGAACATGTAAGGCTTAAGGTCGacat > 1:186094/1‑226 (MQ=255) ttttCT‑GG‑AG‑GTGGTGGCGGCTTCCCGCAATTTCGGGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCAGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGc < 2:186094/228‑1 (MQ=255) tttCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGGAGAAGTAAATAAATCTGGCGGAGC‑CGGGGAGCTCCGCCAGGGCCCTTAAACAGCTGGCATATTGCGCCCGGAATAACTCTCTGGCATTTCTTTCCGCAGCGCAGCGGGAATTTCCTGTCGCCTGAGGTCGGTTAAGTCGTCCGGTTTGGTGGGGAACCTTATGTGCTTAAGGTCGAACACCTTCAGCaa > 2:540287/1‑229 (MQ=255) tttCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGGTTGGTGGGGAACATGTGATGCTTAAGGTGGAACCCTTTacacaa > 2:499560/1‑224 (MQ=255) tttttttggtgtgcggggcgtcTCACGGTAATTTCGTGCAGCAGAAGTAAAAAAAGCGGGGGGCGG‑CTGGGAGCCCCGCACGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAg < 2:688642/212‑1 (MQ=255) cgtgggggcgggggggcTTCCCCAAATTTCGTTCAGCAGAAGAAAAGAACT‑TGGGGGGGCTCTGGAGGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGTCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACAt < 2:647616/215‑1 (MQ=255) ggggcgttacggatatgccGTGCAGGAGAAGAAAAGAATTGGGGGGGAGC‑CGGGGAGCCGCGCAGGAGCCGTTAAACAGCTGGCATAGTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGa < 2:183634/211‑1 (MQ=255) ggcggcTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAGATCTTTAAGCAACATCTTG‑GTATgg > 1:602252/1‑228 (MQ=255) ggcgcgttcccGCAATTTGGGTCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGCGCCCCGCCAGGGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGa < 1:72759/220‑1 (MQ=255) ttACCGCAATTTCGTGCAGCAGAAGGAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCCTATTGGGACCGGAATAAATCTCGCGGATTTCTTTTCCCAGCGCAACGGATATTTCCTGCGCCCCGCCGTGGGTTTAGTCTTCCGGCTTGGGGGGGGAAAGTGATGGCTGTAGCTCGGACTCTTTTATGAAAGACTTG‑GGATGGAAGatat > 2:497355/1‑229 (MQ=255) ttgcggccagaagAAGTAAATAAATCTGGCGGAGC‑TGGGAACCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGa < 1:108222/221‑1 (MQ=255) tGCAGCAGAAGTAAATAAATCTGTCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATATCGCGCGTTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAATCTTCCGGATTGGTGTGGAACATGTAGGGCTTATGGTCGAACTCTTTAAGCAACATCTTT‑GTTTGGTAGGTGTTTTCCTGCTAGAc > 1:728832/1‑228 (MQ=255) gcagAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCACCGCCAGAGCCGTTAAACAGCTGGCATAGTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTca < 1:87094/229‑1 (MQ=255) aaGTAAATAATTGTCGCCGAGC‑TGGGGATCCCCGCCCGAGCCGTTAAACAGCTTGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCACCATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATc < 1:702844/229‑1 (MQ=255) gtaaatatgttggggggggC‑CTGGGGGCCCCCCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATcca < 2:195196/211‑1 (MQ=255) taaataatGCGGGCGGTGC‑CTGGGATCTCCCCCAGAGCCTTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGACCTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATcca < 1:497355/228‑1 (MQ=255) taaataaaTCGGGCGGATC‑CTGGGAGCTCCGCCGGGGCCGTTAAACAGCGGGCATATTGCGCCCGTAATAAAGCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATcca < 1:399512/228‑1 (MQ=255) taaataaaGTTGGGGGAGC‑CGGGGAACTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCACGTAATAAAGCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATcca < 1:609046/228‑1 (MQ=255) gagacaggtCTGGCGGTGC‑CTGGGTGCTCCGCCAGGGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATcca < 1:409309/220‑1 (MQ=255) aaataattTGGGGGGGGC‑CTGGGCGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATccacc < 2:446867/229‑1 (MQ=255) aataatgtgggCGGGGC‑TCGGGAGCCCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATccacc < 2:423893/219‑1 (MQ=255) ggCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCAGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGGAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGGTTGGACCATGTAGGGCTTAAGGTCGAACTCTTTAAGCAAC‑TATTGTGGATGGAAGAAATTTTCCCGTTCGACGtctccacacaccacctcatcaa > 2:494845/1‑208 (MQ=255) ggggggC‑CTGGGGGCCCCCCCAGGGCCCTTAATCAGCGGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAg < 2:640268/224‑1 (MQ=255) c‑cTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGCAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGGAGATATTTTCCTGTAAGACGTTCACATCTATCAttccactctggcctct > 2:409309/1‑214 (MQ=255) tggggtgtCCGCGAGAGCCGTTAAACAGCTGGCACATTGCCCCCGTAATAAATCCCGCGGGTTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCa < 2:532121/222‑1 (MQ=255) | TCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCT‑GC‑AG‑GTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGC‑CTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTG‑GTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCA > NC_000913/134550‑134996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |