BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,463,875	T→G	15.7%	H279P (CAC→CCC)	treC ←	trehalose‑6‑P hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,463,875	0	T	G	15.7%	235.9 / 6.9	96	H279P (CAC→CCC)	treC	trehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand): ref base T (25/54); new base G (13/4); total (38/58)
Fisher's exact test for biased strand distribution p-value = 9.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCA  >  NC_000913/4463641‑4464109

tttCCCGGCATGCCATTCGGCCCCTTCGCCAGCATTTTTGCCGCAGGCACGCGGTTTTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGtgatg                                                                                                                                                                                                                                               <  1:136989/238‑1 (MQ=255)

gTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGtgatg                                                                                                                                                                                                                                               <  2:880830/239‑1 (MQ=255)

cttcccttccttccctcccccaCCATCGCCAGCATTTTTGCCGCAGGCCCGCGGTATTCCCCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGtgatg                                                                                                                                                                                                                                               <  1:844259/219‑1 (MQ=255)

 ttCCCTGCATGCCATGCAGCCCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGtgatg                                                                                                                                                                                                                                               <  2:325348/238‑1 (MQ=255)

 ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAAAGTTTTCAAAGCCACAAAACCAGGTTTAGCCAGCGTCCATTTTTCACCCCCCGGGAAATCTACCTTCAAGggatga                                                                                                                                                                                                                                              >  2:803111/1‑239 (MQ=255)

   cccTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCCAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAACCACCTTCAGTGGATGaaa                                                                                                                                                                                                                                            >  2:123129/1‑239 (MQ=255)

       tctttccttcacgccccATCG‑CAGCATTTTGACCGGCGGGACGCGGTAGTGCCCTTCATCACCAAAGCGAGAAAACATGCGCGGTTGCTCGTGGATACTCCGGAACAAGGCATTCCATGCTACGTTGTGCAGTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTGTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑Taa                                                                                                                                                                                                                                      <  1:233561/224‑1 (MQ=255)

             cattgcgcaCCATCGCCAGCTTTTGTGCCGCAGGCACGGGGTATTCAGCTTCATCACCAAAGCCAGAAACAATGCGCGTCTGATCGTTGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTc                                                                                                                                                                                                                                 <  2:883734/233‑1 (MQ=255)

              atgcAGCCCAATCGCCAGCATTTTTGACGAAGGCACGCGGTATTCACCTTCAGCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTc                                                                                                                                                                                                                                 <  2:108685/238‑1 (MQ=255)

               tgaagcaCCATCGCCAGCATTTTTGCCGGAGGCACGCGGTATTCCCATTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑Taa                                                                                                                                                                                                                                      <  1:913314/229‑1 (MQ=255)

                     aCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGa                                                                                                                                                                                                                            <  2:733237/236‑1 (MQ=255)

                     aCCATCCCCCGCCTTTTTGCCCAAGGCCCGGGGTATTCACCTTCAACCCCAAAGCGAGAAACAATGCGGGGCTGATCGTGGTTACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGa                                                                                                                                                                                                                            <  1:483727/236‑1 (MQ=255)

                       ccccGCCAGCATTTTTGCCCCAGGCCCGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAAtt                                                                                                                                                                                                                       <  1:414058/236‑1 (MQ=255)

                       cATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATGAAAAT‑TAAAGGTCATCGACAAt                                                                                                                                                                                                                        >  2:414058/1‑238 (MQ=255)

                         tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCACCACCCCCAAATCAGGTTTAGCCAGGCGCCATTTTTTAACCCCGGGGTAAACCAACTTCCGGGGGAGAAAAA‑TAAAGGttcacgccaattac                                                                                                                                                                                                                     >  1:990649/1‑226 (MQ=255)

                         tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGGTTAGCCAGCGTCCATTTTTCCCCACCGGGATAATTGACCTTCAAGGGATGAAAAT‑TAAAGGTCATCGACAAtta                                                                                                                                                                                                                      >  2:71704/1‑237 (MQ=255)

                         tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAAAGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTTACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCgccctgtct                                                                                                                                                                                                                     >  2:913314/1‑231 (MQ=255)

                            gcAGCATTTTTGCCGCCGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTg                                                                                                                                                                                                                  <  2:798177/238‑1 (MQ=255)

                                 aTTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAAAGCCCCACAGTCAGGTTTTGCCAGGGGCCATTTTTCACCCCCGGGATAAACGACCTTCCGGTGATGAAAATATAAAggaatcgcaaaatcaataccctt                                                                                                                                                                                                              >  1:600975/1‑218 (MQ=255)

                                   tttgtccGCGGGGACGCGGTGTTTACCTTAAACACCAAAGCGAAGAAAAATGGGCGGCTTATCGGTGTTACGCCAGAACAAGGCATTCCAGGCTACGGTGGTCAGTCCTTGTTGCCAGGGGCGGAAGAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGCCCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCa                                                                                                                                                                                                          <  1:529204/234‑1 (MQ=255)

                                           cAGGCACGCGGTATTCCCCTTCATCCCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCcgcg                                                                                                                                                                                                  <  2:990649/239‑1 (MQ=255)

                                                       ttttgccttcccccccAAAACGAGAAACAATGCGCGGCTCATCGTGGTTACACCAGAACAGGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                       <  1:803111/224‑1 (MQ=255)

                                                       agtCACCTTCAACACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                       <  2:910126/236‑1 (MQ=255)

                                                           cccTTCAACACCAAAGCGAGAAACAATGCGCGGCTGATCGGGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑t                                                                                                                                                                                 <  2:526740/238‑1 (MQ=255)

                                                           aCCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCGTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGaa                                                                                                                                                                                                                                             <  2:885284/182‑1 (MQ=255)

                                                                 atcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGc                                                                                                                                                                           <  2:600975/239‑1 (MQ=255)

                                                                             agaAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGGTAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGCCAAATCACTG‑CCTGTCAAAGCCGCGTATCGCTGGCAATGC‑TCAAAGCTGGGGGAGGACa                                                                                                                                                               >  2:9547/1‑239 (MQ=255)

                                                                             agaAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGGAAAT‑TAAAGGTCATCGACAATTTACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCCAGGCTGGTGGGGGACa                                                                                                                                                               >  2:170160/1‑239 (MQ=255)

                                                                               aaaCAATTCGCGGCGTTTCGTGGTTACACCCGAACAAGGCCTTCCATGCTACCTTGGGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACAt                                                                                                                                                              <  1:71704/238‑1 (MQ=255)

                                                                                      gcgcGTCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTa                                                                                                                                                      <  2:915151/239‑1 (MQ=255)

                                                                                       cgcgGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGGTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTAc                                                                                                                                                     <  2:360822/239‑1 (MQ=255)

                                                                                            cTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCACGGCTGGTGGAGGACATTTCACCCACGGGCa                                                                                                                                                >  2:528819/1‑239 (MQ=255)

                                                                                               aTCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCAAAGTCAGGTTTTGCCAGCGTCCATTTTTCCCCACCGGGGTAAACCACCTTCAGGTGATGAAAAT‑TAAAGGTCCTCCACAATTCACTGCCCTGCCA‑AGCCCCGTCACCCTGGCAATTC‑CTAAGGGTGGGGAGGGACATTTTCCCTCCGGGCCTT‑a                                                                                                                                            >  1:999239/1‑239 (MQ=255)

                                                                                                       tatcccAGAACAAGGCCTTCCATGGTTCCTTGGTCAATCCTTGTGTCCAGGGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGGTGAGCCAGCGTCCATTTTTCACCACCGGGATAAGCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATGCACTG‑CCTGTCAGAGCCGCTTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGt                                                                                                                                    <  1:251351/235‑1 (MQ=255)

                                                                                                       tACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGt                                                                                                                                    <  2:96547/239‑1 (MQ=255)

                                                                                                       tACAGCAGAACAAGGCATTCCATGCTACGTTGGGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCCTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGt                                                                                                                                    <  2:404779/239‑1 (MQ=255)

                                                                                                       tACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCcgcg                                                                                                                                     <  2:580628/238‑1 (MQ=255)

                                                                                                       tACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCcgcg                                                                                                                                     <  2:830893/238‑1 (MQ=255)

                                                                                                       tACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGt                                                                                                                                    <  2:105912/239‑1 (MQ=255)

                                                                                                       tACACCAGAACAAGGCATTCCATGCTACGGTTTGCATTCCTTGTTGGCAGTGGCGGAAAAATGTTTTCAACGCCTAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGt                                                                                                                                    <  2:70097/239‑1 (MQ=255)

                                                                                                         cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACCCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACAACCGCGATAATCGACCTTCATGGTATGAAAAT‑TAAAGGGCATCGCCAATTCACTG‑CCTCTCACAGCCGCGGATCCCTGTCAAATC‑TCAAGGCCGGTGGAGGACATTTCACCTACCGTACTT‑ACCCCGCGTg                                                                                                                                   >  2:962697/1‑238 (MQ=255)

                                                                                                              gAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCAACGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGGCCTTCAGGGGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑GCTGTCAAAGCCCCGTATTGCTCGCACAGG‑TCAAGGCTGGTGGAGGACAATTCACCTACGGTCCTT‑TACCCCCGTgtggcaa                                                                                                                             >  2:834742/1‑233 (MQ=255)

                                                                                                              gAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGGCAATTAACTG‑CCTGTCAGAGCCGCGGATCGCTGGCAATGC‑TCAAGGCTGGGGGAGGGCATTTTACCTACGGCCATT‑AAACCCCGGGGCGC‑aa                                                                                                                            >  2:357371/1‑239 (MQ=255)

                                                                                                              gAACAAGGCATTCCATGCTACGTTGGGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTTAACGCCACCAAATCTGGTTTAGCCAGCGTCCATTTTTTACCACCGGGCAAATAGAACTTCAGGGGAGGAAAAA‑TAAAGGTAATCTAAAACTCACAG‑CCTGTCAAGGCCCGGTATCCCCGGCAAATA‑CCAAGGTAGTGGGGGGACATTTCACCTACggggataacaacccgtgggggaa                                                                                                                              >  1:250753/1‑218 (MQ=255)

                                                                                                               aaCAAGGCATTCCGTGCTCCGTTTGGCATTCCTTGTTGCCAGGTGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGTCCTGCAGGTGATGAAAAT‑TAACGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑aaa                                                                                                                           <  2:49816/239‑1 (MQ=255)

                                                                                                               aaCAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCCGGATAATCGACCTTCAGGTGGTGAAAAT‑TTAAGGTCCTCGCAAATTCACTG‑CCCGTCAGACCCGCGGATCGCTGGCAATGCTTCCAGCCGGGGGGGGGCCATTT‑ACCTACGGGCCTT‑TACCCCCGGAGCGa‑aaa                                                                                                                           >  1:668083/1‑239 (MQ=255)

                                                                                                                    ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCCCCAAGTCAGGTTTAGCCAGCGTCCATTTTTTACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGGCATAGCCAATTCACTG‑CCTGTCAAAGCCCCGTATCGCTGGCAATGC‑TCAAGGCGGGGGGGGGAGATTTCACCTACGGGCATT‑AAACCCCGGGGGGa‑aaaaaaa                                                                                                                       >  1:532552/1‑234 (MQ=255)

                                                                                                                    ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAAGCTGGTGGAGGACATTTCACCCACGGTCATT‑AACCCTCGTGGCGT‑AAAaaca                                                                                                                       >  2:270113/1‑238 (MQ=255)

                                                                                                                    ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAAAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGGGGAGGAACTTTTACCTACGGGCCTT‑AACCCCCGGGGGGT‑aaaacaa                                                                                                                       >  2:841776/1‑234 (MQ=255)

                                                                                                                    ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATGAAAAT‑TAAAGGGCATCGGCAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGGGGAGGACCTTTCACCTACGGTCATT‑AAccccccggggggaaaaaca                                                                                                                        >  2:395840/1‑222 (MQ=255)

                                                                                                                    ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAACCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCCCGTATCGCTGGCAATGC‑TCAAAGCCTGGGGAGGGCATTTCTCCTCCGGTCATT‑TAACCACGTGGCGC‑AAaaacc                                                                                                                       >  2:24400/1‑237 (MQ=255)

                                                                                                                      cATTCCATGCTACGTGGTGCATTCCTTGTTGCCAGTGGCGGAACAATTTTTGCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACAt                                                                                                                      <  2:1019562/237‑1 (MQ=255)

                                                                                                                             tGCTACGTTGTGCATGCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTa                                                                                                                                                      <  2:565957/200‑1 (MQ=255)

                                                                                                                               ttcgtttGTGCTATCCTTTTTGCCAGTGGCGGACCAGTGTTTTCAACGCCACAAATGCCGGTTGAGCCAGCTTCCAGTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCAtc                                                                                                           <  1:267073/234‑1 (MQ=255)

                                                                                                                                 aCGTTGTGCATGCCTTTTTGCCAGTGGGGGAAAAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCCCCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCAtctc                                                                                                         <  1:841776/239‑1 (MQ=255)

                                                                                                                                     tgtgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGGCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGAAATGC‑TCAAGGCTGGTGAAGGACATTTCACCTACGGTCATT‑AACCCGCAGGGCCG‑AAACACATccgaggtcaatttcggc                                                                                                     >  2:260965/1‑223 (MQ=255)

                                                                                                                                       tgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTATCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCCCTG‑CCTGTCAGAGCCGCGTATCGCTGGCACTGC‑TCAAGGCTGGTGGAGGACATTTCCCCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCCCGGTCCATCTC‑GCGCac                                                                                                  >  2:378447/1‑238 (MQ=255)

                                                                                                                                       tgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTa                                                                                                                                                      <  1:324988/190‑1 (MQ=255)

                                                                                                                                       tgCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAACGACCTTCAGGGGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTa                                                                                                                                                      >  2:324988/1‑190 (MQ=255)

                                                                                                                                         ctttCCTTGTTGCCAGGGGCGGAAAAATTTTTTTAACCGCACAAATGCAGGTTTAGCCAGCGTCCATTTTTCACCCCCGGGAAAAACGCCCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCaaa                                                                                                 <  1:24400/236‑1 (MQ=255)

                                                                                                                                         cATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGGAAAGC‑TCAAGGCTGGGGGAGGGCATTTCACCTACGGTCCTTAAACCCGC‑TGGCGG‑AAACACACTCCGGTTcgatcccggcaaaa                                                                                                 >  2:295313/1‑226 (MQ=255)

                                                                                                                                         cATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTTACCTACGGGCATT‑AACCCCCGTGGGGG‑AAACAAATCCCGGTTTATCCC‑GTGCaaaa                                                                                                >  2:240140/1‑239 (MQ=255)

                                                                                                                                            tCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTTGCCAGCGTCCATTTTTTACCACCGGGATAAAAGACCCTCCGGGGATGAAAAT‑TAAAGGTTCTCGACAATTCACTC‑CCTGTCCAAGCCCCGTATCCCTGGCAATGA‑TCAAGGCTGGTGGCGGGCATTTGACCTACCGCCATT‑AATCCGCGTGCGGT‑AAAAACACAGCGGtgctacccgggccaaaaaa                                                                                              >  1:408283/1‑221 (MQ=255)

                                                                                                                                                ttttgCCTGTGGCGGAAAAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCCGGTGCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCgtg                                                                                         <  1:707438/237‑1 (MQ=255)

                                                                                                                                                ttgtcccAGTGGCCGAACAATGGTTCTAACGCCACAAAGTCCGGTTTAGCCATGGTCCATTTCTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCgtg                                                                                         <  2:773433/234‑1 (MQ=255)

                                                                                                                                                tgttgCCGGTGGCGGAACAATTTTTCCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCCCCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCgtg                                                                                         <  2:250046/239‑1 (MQ=255)

                                                                                                                                                 gttgCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTGCCTTTTTCACCACCGGGATAATCGACCTTCAGGGGATGAAAAT‑TAAAGGTCATCGACCATTTACTG‑CCTGTCCAAACCCCGTATTACGGGCAATGC‑CAAAAGTGGGTGTGGGGCATTTCACCCACCGTCATT‑AACCCCCG‑GGCGTGAAACAAAACCGGGTTCATCCCTGTGCAAAAAccgggg                                                                                         >  1:747092/1‑234 (MQ=255)

                                                                                                                                                 gttgCCAGTGGCGGAACAATGTTTTCAAAGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGGTGAAAAT‑TAAAGGTCATCGACAATTCACAG‑CCTGTCAGAGCCCCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGGAAATTCACCTACGGTGATT‑AACCCCCGGGGCGG‑AAACCAAACGCGGGTTATCCC‑GTGCaaaaaaccacgg                                                                                        >  1:658534/1‑232 (MQ=255)

                                                                                                                                                              gtaacattttttGAACGCCAAAAAGTTAGGTTTAGCCAGTGTCTATTTTTCACCACCGGGGTAATCGACCTTCAGGGGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGACCGt                                                                           <  1:378447/231‑1 (MQ=255)

                                                                                                                                                              gaaataTGTTTTCAACTCCACAAAGTGCGGTTTAGCCAGCGTCCATTTTTCACCTCCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCAGCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGGGGCGT‑AAACACCTCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                           <  2:824732/234‑1 (MQ=255)

                                                                                                                                                               aaCAATTTTTTCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                           <  2:900365/238‑1 (MQ=255)

                                                                                                                                                               aaCAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                           <  2:945996/238‑1 (MQ=255)

                                                                                                                                                                aCAATGTTTTCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCg                                                                         <  2:658534/239‑1 (MQ=255)

                                                                                                                                                                 cAATGTTTTCAACGCCACAAAGTCGGGTTTAGCCCGCGTCCATTTTTCCCCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCgg                                                                        <  2:412310/239‑1 (MQ=255)

                                                                                                                                                                     gTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCACCGACAAGTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTa                                                                    <  2:293838/239‑1 (MQ=255)

                                                                                                                                                                            aCGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCCTGGCGT‑AAACACATCGCGGTTCATCTC‑TTGCAAAAACTCGGTTGCTCGTGGCCCGCAGGTGTAGAAGCg                                                              >  1:730967/1‑238 (MQ=255)

                                                                                                                                                                                cacaAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                       >  2:374676/1‑117 (MQ=255)

                                                                                                                                                                                cacaAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                           >  2:674836/1‑221 (MQ=255)

                                                                                                                                                                                cacaAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                           <  1:674836/221‑1 (MQ=255)

                                                                                                                                                                                caaaaaGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCa                                                                                                                                                                                       <  1:374676/114‑1 (MQ=255)

                                                                                                                                                                                     atttaGGTTTAGCCAGCTTCCATTTTCCACCACCGGGATAATCGGCCTTCAGGGGATGAAAAT‑GAAAGGTCATCGACAATTCACTG‑CCTGGCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                    <  1:528819/235‑1 (MQ=255)

                                                                                                                                                                                     aggcAGGTTTGACCGGGGGCCATTTTTCCCCACCGGGATAAACGACCTTCAGGTGATGGAAAT‑TAAAGGTCATCGAATATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                    <  1:170160/236‑1 (MQ=255)

                                                                                                                                                                                     aGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                    <  2:532552/239‑1 (MQ=255)

                                                                                                                                                                                     aGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                    <  2:240017/239‑1 (MQ=255)

                                                                                                                                                                                          ggTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGCGGACATTTCACCTACGGTCATT‑AACCCGCGt                                                                                                                                    >  1:803415/1‑156 (MQ=255)

                                                                                                                                                                                          ggTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGt                                                                                                                                    <  2:803415/156‑1 (MQ=255)

                                                                                                                                                                                             ttAGCCCGGGTCTATTTTTCCCGACCGGGATTAACGACCTTGAGGTGATTAAAAG‑TAAAGGTCATCAACAAGTCGCTG‑CCTGTCAGAGGCGCGGAGCGCTGGCAATGC‑TCAAGGGTGGTGGAGGACATTTCACCTTCGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCa                                             <  1:270113/238‑1 (MQ=255)

                                                                                                                                                                                             ttAGCCAACGTTCTTTTTGCACCACCGGGATAAGCGCCCTTCAGGTGATTAAAAT‑TCAAGGTCAGCGATAATTTCCGG‑CCTGTCTGAGCCGCGTCTCGATGTAAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACCCATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCTCGGCGGTGTAGAGGCGACGGACGTCGCCGGCCAg                                            <  1:123129/239‑1 (MQ=255)

                                                                                                                                                                                               aGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AAACCGCGTGGCGT‑GAACACATCGCGGTTCATCTC‑TGGCAAAAACTCTGGGGCCGGTGGCCCGGCGGTGTAGAAACGAGCCCCGtgcccggcccgag                                          >  1:917896/1‑227 (MQ=255)

                                                                                                                                                                                                     cGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGGGTAAAAGCGACGCCCTTCGCCGTCCAGGTCTT‑CAg                                   >  2:102030/1‑239 (MQ=255)

                                                                                                                                                                                                            ttttCTCCCCCGGGATAAGCGCACCTCCGGGGGTGAAAAT‑TGAGGGTCATCAACAATTCACTG‑CCTGTGAGAGCCGCGTAGCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAAcg                            <  1:834742/239‑1 (MQ=255)

                                                                                                                                                                                                            ttttCCCCCCCGGGATAATCGGCCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGGGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAAcg                            <  2:484985/239‑1 (MQ=255)

                                                                                                                                                                                                            ttttCCCCCCCGGGATAATCGCCCTTCAGGGGAGGAAAAT‑TAAAGGTCACCGACAATTCCCTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAAcg                            <  1:569477/239‑1 (MQ=255)

                                                                                                                                                                                                              ttCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAAcgcg                          <  2:1000649/239‑1 (MQ=255)

                                                                                                                                                                                                                     ccGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCCAGGCTGGTGGAGGACATTTCACCTCCGGTCATT‑AACCCGCCTGGCGT‑AAACCCATCGCGGTTCATCTC‑GTGCAAAAAATCGTGTGCCCGTGTCCCGGCGGGGTATAAACCACGCCCGACGCCGCCAAGGACTTACGAGGAAACGggggcgtg                    >  1:369069/1‑235 (MQ=255)

                                                                                                                                                                                                                     ccGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGCCGGTGTGAAAGAGACTCCCGGCGCCGTCCAGGGCTT‑CAGGGAAACGCgggctctg                   >  1:789503/1‑234 (MQ=255)

                                                                                                                                                                                                                      cGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAACGCGGGTCTTTg                  >  2:840745/1‑239 (MQ=255)

                                                                                                                                                                                                                      cGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAACGCGGGTCTTTg                  <  2:668083/239‑1 (MQ=255)

                                                                                                                                                                                                                      cGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAACGCGGGTCTTTg                  >  2:569477/1‑239 (MQ=255)

                                                                                                                                                                                                                                      cAGGTGAGTAAAAG‑TAAAGGTCAGCGACAATTCACGG‑CCTGTAAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAACGCGGGTCTTTGGAGATCAGATTcacca  <  2:695502/239‑1 (MQ=255)

GTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGAACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATGAAAAT‑TAAAGGTCATCGACAATTCACTG‑CCTGTCAGAGCCGCGTATCGCTGGCAATGC‑TCAAGGCTGGTGGAGGACATTTCACCTACGGTCATT‑AACCCGCGTGGCGT‑AAACACATCGCGGTTCATCTC‑GTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTT‑CAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCA  >  NC_000913/4463641‑4464109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`