| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,561,718 | T→C | 16.8% | G35G (GGA→GGG) | glpE ← | thiosulfate:cyanide sulfurtransferase (rhodanese) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,561,718 | 0 | T | C | 16.8% | 145.5 / 4.5 | 63 | G35G (GGA→GGG) | glpE | thiosulfate:cyanide sulfurtransferase (rhodanese) |
| Reads supporting (aligned to +/- strand): ref base T (38/13); new base C (3/9); total (41/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCT > NC_000913/3561483‑3561952 | aCAGTATAAAGCGTTCCGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTcc < 2:713740/238‑1 (MQ=255) tataAAGCGTTACGCGCCGTACGCCGCCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCAtt > 2:173781/1‑238 (MQ=255) taaacggttccccgcCGTCCCCCCCCTCTGGGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTACAGACCACATCGTAGCCCTG‑TTGCAGCAGTTACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCg < 1:10814/227‑1 (MQ=255) taAAGGGTTACGCGCCGTACGCCACCCCTGGTGGAATCTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTAGAGCCCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCTCGCATAAAAGCGCCGAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCg < 2:107416/239‑1 (MQ=255) taAAGCGTTCCGCGCCGTACGCCCCCTCGGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCg < 2:574374/239‑1 (MQ=255) taAAGCGTTACGGGCCGTCCGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCg < 2:670359/239‑1 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAACGCCGCCGTCAATGCTATAGACCACATCGTCGCCCTG‑TTTCAGCAGCTACTGCCCCGCGCCTTTGCTTCTTTTTCCGTGTTACCTAATCACCTTCACCGGCCGGTCAAAGTCGTTATCACGCATAAAGCCGCCCACCGGTTCGTCG‑TTTAAATGGAAAGCCTCACCCCCATGTCACATTGCGCAACTC‑t‑g > 1:71780/1‑239 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGTTAACACATCACCATCACCGGAGTGTCAAAGGCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCAAGTCCCATTGCGCAACGC‑t‑g > 2:147569/1‑239 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGTAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑t‑g > 2:890462/1‑239 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑t‑g > 2:573547/1‑239 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCCCATGTCCCATTCCGAAActc‑tt > 2:240988/1‑238 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGAAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑t‑g > 2:227589/1‑239 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTATTCACGCATAAAAACGCCAAGCGTGTCGTTG‑GTTAACTGGAAAACCGGCACCGCATGTCCCATAGAGAAAatcgg > 1:215870/1‑237 (MQ=255) tACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACACCACCATCACCGGAGTGTCAAAGACGTTGTCGCGCCTCAAACCACCCCGCGTGTCGGTG‑GTTAACAGGAAAGCCTTCACCACAGCCCCCATAGCGaaaagcgt > 1:766999/1‑234 (MQ=255) tACGCGCCGTACGCCACCTCTGC‑GGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAACCCTGCACCGCATGCCCCATTGCGAAAccc‑ttt > 2:389643/1‑237 (MQ=255) cgcgCCGTACGCCCCCCGTTCGGGAAACTGAGGTGGCCAGGCTTCAAAGCCGCCGCCAATGCTAGAGACCACATCTTAGCCCGG‑TTGCAGCAGATACGGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACGCATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑t‑gt‑g < 1:627004/239‑1 (MQ=255) cGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGCAAGCCTGCACCGCATGTCCCATTGCGCAACTC‑T‑GT‑GGATc > 2:383632/1‑238 (MQ=255) cccccTCCGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGGCCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑Aata < 2:609727/236‑1 (MQ=255) ctctGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGCCCCATTGCGAAACCC‑T‑GT‑GGTTCGCG‑AAAATca > 2:529906/1‑237 (MQ=255) ctGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGAcc > 2:462183/1‑239 (MQ=255) gCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCCCG‑AATATCGACCAG‑CACCGCCTCTTTTTc > 2:386495/1‑238 (MQ=255) gCTATACACCCCATC‑TTGCCCTGTTTACAGAAGATCCTGCGCCGCGCTTTGGCTTCTATTGCCGTTAAAACACACCCCCATCCCCGGGGTGTAAAAGGCGTTACCCCAAAAAAAAGCGCCCGGCGTGTCGTGG‑GTTAAATGAAAAGCCTGCCCCCCATTCCCCATTGAGAATCTC‑T‑GT‑GGAGCGCG‑AATACCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGc < 1:503430/237‑1 (MQ=255) ataGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGt > 2:556968/1‑239 (MQ=255) caTCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGt > 2:655225/1‑216 (MQ=255) aTCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑Aata > 2:620281/1‑178 (MQ=255) aTCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑Aata < 1:620281/178‑1 (MQ=255) aTCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCACCTTCTGGGGCGCGCCGGCaaacgtaaaaaaac > 1:618141/1‑227 (MQ=255) aTCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATa > 2:503430/1‑235 (MQ=255) aTCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAACCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCCCTTTTTCCTGCCACTTCTGTTGCGCGCCGGCACCGTTAATAcct > 1:541746/1‑236 (MQ=255) cccTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGGGCGCGCCGGCAACGTTAATACATTCGAACTg > 2:244955/1‑239 (MQ=255) ttGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGCCCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGACCCa > 2:846846/1‑239 (MQ=255) ttGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATc > 2:707305/1‑237 (MQ=255) gcgccTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGCGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑TTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GAACGGCG‑AATATCGCCAAG‑ACCCGCCCCTTTTCCCTGCAACCTCGGGTGCGCGCTGGCCCACTTAATAACTTCGAACTGCGCCACTCGCATTCCTTTCTTTac > 1:572910/1‑239 (MQ=255) tggtgttatttccttaaaaaCACACCACCGTCACCGGAGTGTAAAAGTCTGTATCCCGAAAAAAAGCGCCAAGGGTGGCGTGG‑GTTAAATGGAAAGCCTGCACCGCAGGCCCCATTGGGAACTGT‑T‑GG‑GGATGGGG‑AATACCGACCAG‑CCCCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAg < 1:173781/221‑1 (MQ=255) aTTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GG‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCaa > 2:809872/1‑239 (MQ=255) gTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTCAAAACAAGTGGGCAAATTTa > 2:198542/1‑238 (MQ=255) aTAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATTTACCAG‑CACCGCCTCGTTTTCTTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCTTCTCTCTTTTTTTACAAAAAAATGGGCAAATTTAccac > 1:163023/1‑237 (MQ=255) aTAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACAAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGc > 2:164156/1‑239 (MQ=255) aaacacctccaccgccccGGTGTGTGAAATGCTTTACCACACAAAAAAGCCCCCAG‑GGGTGGGTGTGTAAAATGGAAACCCCTCCCCGCTTGCCCCTTTGCG‑AACTG‑TGGG‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGca < 1:707305/223‑1 (MQ=255) caccatcaccGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTAc > 2:772150/1‑239 (MQ=255) caccatcaccGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCAACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTTGTGCGCGCCGGCAACGTTAATACATTCGAACTGATCCATGAGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTAc > 2:419166/1‑239 (MQ=255) gtgtCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGGGCGCGTCGGCAACGTTAATACATTCCAACTGATCCCTGCGTCTCTCTTTTTTTACAAACAAGAGGGCAAATTTACCCCAAAGTTTACGTCGacagggaag > 1:731222/1‑231 (MQ=255) tCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCCCTCTTTCTTTCCAACAAAGGGGGCAAATTTACCGCCAAATTTAACTGGAACCGGCaaaaa > 1:53381/1‑235 (MQ=255) aaaGTCGTTACCCGGCAAAAAGCCGCCCAGGGTGTCGTTG‑GTTAAGGGGAAACCCCGCCCCGCATGTCCCATTGGAAACCTT‑T‑TG‑GGATCGGA‑AATTTCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATaaa < 2:618141/239‑1 (MQ=255) ttttaccccgaaTAAAAGC‑CCCAGGGTGGTGTTT‑GTTAAAAGGAAAACCCTCCCCCGATGTCCCCATGCAAAAC‑C‑T‑GTGGGGGTGCGAAAAATCCACCAC‑CCCCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCa < 1:556968/228‑1 (MQ=255) ctttctcccgtaaaaaaGCGCCCGGGGTGTGTTTG‑GTAAAATGGAAAGCCTCGCCCGTATTCCCCATGGC‑AAACTCTT‑GG‑GGATGGGA‑AATATCGCCCGG‑CCCCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCa < 1:207631/226‑1 (MQ=255) ctttatccggaataaagccccccAGGATGCCGTTG‑GTAAAATGGAACCCCTGCACCGCATGCCCAATTGCGAAACTC‑T‑GT‑GGATCGCA‑AATTTCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATGAACGCCa < 2:124111/219‑1 (MQ=255) ctttaccgcGCAAAAAGCCGCCCAGCGGTTCGTGG‑GTAAATTGGAAAGCCGCCCCCGAAGGCCCCATTGCGAATCTT‑T‑GT‑GGACCGCG‑AATATCGCCCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCa < 2:541746/231‑1 (MQ=255) ctttATCACGCAAAAACGCGCCCAGCGTGTCTTGT‑GTAAAGGGGAAAGCCTGCACCGCATTCCCCTTTCCAAACCTC‑T‑GT‑GGTTCGCA‑ATTAGCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCa < 2:454114/237‑1 (MQ=255) cGTTACCCCCCAAAAAAGGCCCCGGCGTTTCTGTG‑GTTAAAGGGAAAGCCCGCACCGCATGCCCCATTGCAAACCTC‑T‑GG‑GGATCGGA‑AATACCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCa < 2:47660/239‑1 (MQ=255) tcaccaaaaaaggccgcaggCGTGTGGTTG‑GTTAAAGAGAAACCCGGCAACGCATGCCCAATCGGAAAACTG‑T‑GT‑GAGTAGGG‑AATTCCGGACGC‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATg < 2:766999/220‑1 (MQ=255) cGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTta > 2:201637/1‑239 (MQ=255) cccAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATTTtata > 2:566986/1‑229 (MQ=255) cccAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGGTTACGTCGAAGCGGCAGATAACCGCCATAATGTTATACATATCActc > 2:50655/1‑239 (MQ=255) tCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGtt > 2:871629/1‑239 (MQ=255) gg‑gTAAAAGGGAACCCCTGTCCCGACTGTCCCATTGCAAAACTC‑G‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGtttttt < 2:818267/238‑1 (MQ=255) ttgtaaaaGGGAAACCCTCCCCCCAATCTCCCTTTGCGAAACT‑‑T‑GTGGGAGCCAG‑AATCGCCGCAAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGtttttt < 1:846846/234‑1 (MQ=255) aaaTGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACCTATCACCCTAAAATGTTTTTTCAATg > 2:766724/1‑239 (MQ=255) aaGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCCCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATAAATATACCCCTAAAATGTTTTTTAAATTTTACATa > 1:250391/1‑239 (MQ=255) cccATGTCCCATTGGAAAACCT‑T‑GT‑GGTTCCGG‑AATTGCG‑CCAGAGACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGAtt < 2:665387/237‑1 (MQ=255) cATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCCCTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCt > 2:804697/1‑239 (MQ=255) cATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCCCTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCt > 2:893010/1‑239 (MQ=255) cATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCt > 2:447514/1‑239 (MQ=255) | ACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGCCGTCAATGCTATAGACCACATCGTAGCCCTG‑TTGCAGCAGATACTGCGCCGCGCCTTTGCTGCTATTGCCGTGATAACACATCACCATCACCGGAGTGTCAAAGTCGTTATCACGCATAAAAGCGCCCAGCGTGTCGTTG‑GTTAAATGGAAAGCCTGCACCGCATGTCCCATTGCGAAACTC‑T‑GT‑GGATCGCG‑AATATCGACCAG‑CACCGCCTCTTTTTCCTGCAACTTCTGGTGCGCGTCGGCAACGTTAATACATTCGAACTGATCCATGCGTCTCTCTTTCTTTACAAACAAGTGGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCT > NC_000913/3561483‑3561952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |