Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,487,251	C→T	14.4%	L345F (CTC→TTC)	lptF →	lipopolysaccharide export ABC permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,487,251	0	C	T	14.4%	222.3 / 4.3	99	L345F (CTC→TTC)	lptF	lipopolysaccharide export ABC permease
Reads supporting (aligned to +/- strand):  ref base C (32/50);  new base T (13/2);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 1.23e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGAAT  >  NC_000913/4487018‑4487479                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          acgggCGTATCACGTTGGTATTCACCGTGTTTATGATGGCCTTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAAc                                                                                                                                                                                                                                      <  2:250379/236‑1 (MQ=255)     gCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTACAGGG‑AAACCGGACCCGACG‑CTGGGGGTTGGGAACA‑CTA‑ACCAGATTTATTT‑G‑GGT‑TTAG‑GGATTG‑TTGTAAACCctt                                                                                                                                                                                                                                  >  1:360120/1‑239 (MQ=255)     gCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGTTGTGGGCCG‑CTA‑ACCTGGTTTATCT‑T‑GCT‑TTAT‑CGATTG‑GTTTCAACCttt                                                                                                                                                                                                                                  >  2:379361/1‑239 (MQ=255)          tCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGTCGGTAACGGT‑AAGCTGGGCCCGACG‑CTGTGGATGTGGGCCG‑TTA‑ACCTAGTTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTTTCAACCTTTgggg                                                                                                                                                                                                                              >  2:125886/1‑237 (MQ=255)          tCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACC‑CTGTGGAGTTGGGCCG‑GTA‑ACCTGATTTTTCT‑G‑GCT‑TTTT‑CGATTG‑TTCTCAACCTTTGGG‑ac                                                                                                                                                                                                                            >  2:483033/1‑239 (MQ=255)               ttGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGCAAAGGT‑AAGATGGACCCGACG‑CTGTGGAGTGGGAACG‑GTA‑CCCTGATTTTGTA‑G‑GCT‑TTTA‑TCATTG‑TTTTCGAACTTTggggacccg                                                                                                                                                                                                                         >  1:206077/1‑232 (MQ=255)                   tATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGATAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGCCCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGCTTG‑TTTTCAACCTTTGGG‑ACACCggtccc                                                                                                                                                                                                                   >  2:466433/1‑234 (MQ=255)                        aCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACTCACGTCAGGGACGCGTACTGTCGATGCTGTCAGCCATGTTGCTGTATCTACTTTTCTTCCTGATCCCGACCTCCCTCAAATCGA‑A‑CGGCGGTTTCGGTGAAGC‑GGGGCCAACT‑CCGTGGTCAGGGACCG‑GTA‑TCCTGAATTAACT‑G‑GCT‑TTCC‑GTCGTT‑TTCTCATACTTTGGG‑acacacggacggcgc                                                                                                                                                                                                               >  1:191190/1‑227 (MQ=255)                        aCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑C‑CGGCGGTAAAGGG‑AAACTGGACCCGGCG‑CTGTGGATGTGGCGCG‑GTA‑AACTGCTTTTTCT‑G‑GCT‑TTAG‑AGATTT‑TTTTCAACCTTTTGG‑CGACccgg‑ccgggcac                                                                                                                                                                                                             >  1:194012/1‑235 (MQ=255)                          cGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGA‑TAGCTGGACCCGACC‑ACTTGGATGTGGACCG‑TTA‑CACTGATTTATTT‑G‑GGT‑TTGG‑CGATTG‑TTTTCAAACTTTTGT‑AGAACGTG‑CCGGtgccc                                                                                                                                                                                                            >  1:779687/1‑234 (MQ=255)                            tgtTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGT‑A‑CGGCGGTAAAAGT‑AAACTGGACCCGACC‑CCGTGGATGTGGACCG‑TTA‑ACCCGATTTATTT‑G‑GCG‑TTCG‑CGCTTT‑TTTTTAACCTTTGGG‑AAACCGTG‑GCGGGCcggcgc                                                                                                                                                                                                         >  1:766119/1‑239 (MQ=255)                            tgtTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGTCCCCCCG‑CCGTGGATGTGGACCC‑TTA‑ACCTGATTTATTT‑G‑GAT‑TTAG‑CGCTTG‑TTTTTACCCCTTTGG‑ACACCGTA‑CCGGGgcgccg                                                                                                                                                                                                          >  1:516265/1‑238 (MQ=255)                            tgtTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGGGGATGTGGACCG‑TTA‑ACCTGATTTATTT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGgccgccc                                                                                                                                                                                                          >  2:87401/1‑237 (MQ=255)                                    atgGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATTT‑G‑GCT‑TTTG‑GGATTG‑TTTTCAAACTTTGGG‑ACACCGTG‑CCGGTCCCCCCCCTgcg‑cg                                                                                                                                                                                                 >  2:370498/1‑238 (MQ=255)                                      ggCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGG‑AAACCGGACCCCGAG‑CTGTGG‑GGTGGGGCGATTA‑CACGTCTTTATCT‑T‑GCTGTTAG‑GGTTTT‑TTCT‑AACCTTTGGGAACACCGGG‑CCGGCccgagccccgcgccca                                                                                                                                                                                                >  1:812990/1‑226 (MQ=255)                                      ggCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAAGG‑AAGACGGACCCGCCGCCTGGGGA‑GTGGAGCG‑TTACACTTGATTT‑TCTGG‑GTT‑TTCG‑CGTTTG‑TTTCAAACCTTGGGG‑AC‑CCGGG‑CCgggtcggccccctcgccgcc                                                                                                                                                                                               >  1:71254/1‑221 (MQ=255)                                        cACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTCAAAGT‑AAACTGGAACCGACC‑CCTTGGATGTGGACCG‑TGA‑AACCGATTTTTCT‑T‑GGT‑TTAGAGGATTG‑TT‑TCACCCTTTTGG‑ACACCGTGCCCGGCCCGCC‑CCCGCG‑CGccggg                                                                                                                                                                                            >  1:279268/1‑236 (MQ=255)                                        cACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGGTGAGGTGGACCG‑CTA‑ACCTTATTTTTCT‑T‑GCT‑TTGT‑CGATTG‑TTTTCACCCTTTGGG‑GACCCCTG‑CCGGTCCCCCCCCTCCG‑CGCCa                                                                                                                                                                                              >  1:312482/1‑237 (MQ=255)                                             aTGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCCGGTTTATCT‑G‑GCT‑TTTG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCCTT‑CCGGTCCCCCGCCTTCG‑CGCCCGttttt                                                                                                                                                                                        >  2:389605/1‑238 (MQ=255)                                                   tgacggctgggctgggTTAACCCACTTCAGGGACGCGTACTGTCGATGCTGCCAGCAATGCTGCTGTATCTACTTTTCTTCCTGATCCAGGCCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTa                                                                                                                                                                                 <  2:349619/224‑1 (MQ=255)                                                    tcccGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCa                                                                                                                                                                                                                                        <  2:344622/184‑1 (MQ=255)                                                    tACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑GTG‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGGG‑CCGGGCCGCCCCCTGCG‑CGCCAGTTTTTTGC‑GGaaa                                                                                                                                                                               >  2:346943/1‑239 (MQ=255)                                                    tACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAAGT‑GAACTGGACCCGCCC‑CTCTGGATGGTGAACG‑TTA‑ACTTTCATTTTCTAG‑GTT‑TAAG‑CG‑TTG‑TTTTTAACCCTTTGG‑AAACCGGT‑GCGGGCCGCCGGCCGCG‑CGGCCGATTTTTGC‑GGaaa                                                                                                                                                                               >  1:436852/1‑239 (MQ=255)                                                     aCCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGG‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCCTG‑CCGGTCCGCCGCCTGCG‑GGCCAGTTTTTCCC‑GTAAAg                                                                                                                                                                              >  2:236522/1‑239 (MQ=255)                                                         cTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCTC‑GTAAAGGAGc                                                                                                                                                                          >  2:135373/1‑239 (MQ=255)                                                                 ggTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGGAAAGGG‑AACCTGGACCCCACC‑CTGTGGTTGGGGACCG‑GTT‑ACCTGATTTATTT‑G‑GCT‑TTTG‑CGATTGTTTCTCACACTTTGGT‑ACA‑CGTG‑CCCGTTCGCCGCCCGCGCCGCAAGTTTTTGAC‑GTAAgggggggcggggg                                                                                                                                                                   >  1:768972/1‑229 (MQ=255)                                                                  tttACCCCCCGTCAGGGACGGTTATTGTCGATGCTTCCAGCCAGGCTGCTGTTCTTGCTTTTCTTCCTGATCCAGACCCCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTTTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATg                                                                                                                                                                 <  1:346943/238‑1 (MQ=255)                                                                    taccccccttcggggagcgtggcTGTCGAGGCGGCCAGCAATGCTGCTGTATCTACTTTTCTTCCGTATCCAGCCCTCCCTGAAATCGA‑C‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCa                                                                                                                                                               <  1:858355/217‑1 (MQ=255)                                                                           cGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACC‑CTGGGGATTGGGACCG‑CTA‑AACTGATTTTTTT‑G‑GGT‑TTAG‑AGGTTG‑TTTTCAACCTTTGGG‑GCACCCTG‑GCGGGCCCCCGCCCGCG‑CGCCCGTTTTTCTCGGCAAAGGAgggggggagggaacccttt                                                                                                                                                         >  1:338302/1‑224 (MQ=255)                                                                            ttcagggctctgtctttgcGATGCCCCCAGCCTTGTTGCTGTTTTTATTTTTTTCCCGGACCCAGGCCTCCCTGAAATCGA‑C‑CGGCGGTAAAGGT‑AAGCGGGCCCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTg                                                                                                                                                       <  1:519657/221‑1 (MQ=255)                                                                                    cggttcttttCGTTGTTGCCAGCCTTTCTGTTTTTTCTTTTTTTCTTCCGGATCCAGCCCTCCCGGAAATCGA‑A‑CGGCGGTAAAGTT‑AAGCTGGCCCCGACG‑CTGTTGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACtt                                                                                                                                               <  1:370498/230‑1 (MQ=255)                                                                                       gTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAc                                                                                                                                            <  2:600812/239‑1 (MQ=255)                                                                                             gggagggtcccagccaTGCTTTTGTTTCTATTTTTCTTCCTGATCCAGCCCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGCCCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtat                                                                                                                                      <  1:716935/230‑1 (MQ=255)                                                                                             gctttccccccaggctTGCTTCTTTTTCTTCTTTTCTGACTGACCCAGCCCCTCCTTAAACTGG‑A‑CGGCGGTAATGGT‑ACGCTGGCCCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtat                                                                                                                                      <  1:711363/230‑1 (MQ=255)                                                                                                   ctgcCAGCCATGCTGCTGTATCTTCTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTg                                                                                                                                                       <  2:268165/216‑1 (MQ=255)                                                                                                      cccggcaTGCTGCTTTTACTACTTTTCTTCCGGATCCAGACCTCCCTGAAATCGG‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaa                                                                                                                             <  2:332018/234‑1 (MQ=255)                                                                                                       cagccaTGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaaa                                                                                                                            <  2:875181/239‑1 (MQ=255)                                                                                                                 gctttaacgaCTTTTCCTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTcac                                                                                                                 <  2:61896/230‑1 (MQ=255)                                                                                                                  ctgTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCCTTTGGCGTACTTGACCGCTATATCGGGAAAA‑CAATTTTcacc                                                                                                                >  2:327733/1‑239 (MQ=255)                                                                                                                     ttttttcTTTTCTTCCTGATCCAGACCTCCCTGAAACGGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCGCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCaccacc                                                                                                             <  2:809886/233‑1 (MQ=255)                                                                                                                     tATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCCGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCCGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGTGGTACTTTACCGCTATATCGGGAAAA‑CCATTTTCaccacc                                                                                                             >  2:740825/1‑239 (MQ=255)                                                                                                                       tctttttttCTTCCGGAGCCAGACCCCCCAGAAATGGA‑G‑CGGTGGTAAAGGG‑AAGCGGGACCCGTCG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTAGCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCACCCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACcat                                                                                                           <  1:591329/234‑1 (MQ=255)                                                                                                                       tcttctTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCGGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACcat                                                                                                           <  2:83703/239‑1 (MQ=255)                                                                                                                       tcttctTTTCTCTCTGCGCCAGACCCCCCT‑‑AATCGACACCGGCGGCAAAGGT‑AATGTGGCCCCGACG‑CTGGGGTGTTGGACCG‑TTA‑ACCGGATTTATCT‑G‑GCT‑GTAG‑CGATTG‑TTCTCAACCTTGGGG‑ACGCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCTCCACcat                                                                                                           <  1:455770/239‑1 (MQ=255)                                                                                                                       tCTACTTTTCTTCCTGGTCCAGACCCCCCTGAAATCGA‑A‑GGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACcat                                                                                                           <  2:204046/239‑1 (MQ=255)                                                                                                                         tACTTTTCTCCCTGACCACGACCCCCCGGAAAGCGA‑A‑CGGCGGTAAGGGT‑AAGCTGGCCCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACcatca                                                                                                         <  1:112181/239‑1 (MQ=255)                                                                                                                          aCTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTTGG‑ACACCGTG‑CCGGTCCGCCGCCTCCG‑CGCCAGTTTTTTGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTTACCGCTATATCGGTAAAA‑CCATTTTtaccccactcat                                                                                                        >  2:633978/1‑231 (MQ=255)                                                                                                                               tcttcCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACC‑CCGTGGATGTGGACCC‑GTC‑AACTGGTTTATCT‑G‑GCT‑TTAG‑ACATTG‑TTCTTAACCTTTTGG‑ACACCGTG‑CCGGTTCGCCGCCGTCC‑CGCCCGTTTTTTGC‑GTAAAGGGGCCGTGGGGTGGCACCTTTTGGCGGACTTTGCCGCATAATCGGTAAAACCTATTTTcccccaaacaaaatag                                                                                                    >  1:745936/1‑227 (MQ=255)                                                                                                                                cttcCCGCTCCAGGCCTCCCTAAAAGCGA‑C‑CGGGGGTAAAGGT‑AAGCTGGTCCCGAGT‑CTGTGGAGGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGac                                                                                                  <  1:526814/239‑1 (MQ=255)                                                                                                                                 ttcctcatgcgaccccccgggAAGTCGG‑A‑CGGCGGTAAGCGT‑AAGTGGGCCCCGCCG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTTGCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACGCCGTG‑CCTGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTATGGCGTACTTGACCGCTATATCGGTAAAT‑CTATTTTCACCACCATCATGATGaca                                                                                                 <  1:532496/219‑1 (MQ=255)                                                                                                                                 ttcCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGTTAAAGGT‑AAGCTGGCCCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTTATTTACCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGaca                                                                                                 <  2:812990/239‑1 (MQ=255)                                                                                                                                 ttcCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑CTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑GGCCCGTTTTTCGC‑GTAAAGGAGCGGGGGGGTGCAACCCTTTGGGGTCCTTTACCGCGTTTACTGTGAAA‑CAATTTTtaccaaaaacataataaaa                                                                                                 >  1:171319/1‑225 (MQ=255)                                                                                                                                   cccgctcccgtccTCCCTGACATCGA‑G‑CGGCGGTAAAGGT‑AGGCTGTACCGGACG‑TGTTGGATGTGGCCCG‑TTA‑ACCTGATTTATCG‑G‑GCT‑TTAG‑CGATTG‑TTCGCATCCTTTGGG‑AAACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTTGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACt                                                                                               <  1:229984/228‑1 (MQ=255)                                                                                                                                         ccAGTCCCCCCGTAAATCGA‑A‑CGGGCGTAAAGGT‑AAGCTTGCCCCGGCG‑TTGTGGATTTTGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCAt                                                                                         <  1:236522/239‑1 (MQ=255)                                                                                                                                                 cccTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCCGTTTTTCGC‑GTAAAGGAGCGGGGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCCTGCTGgtg                                                                                  >  2:614621/1‑238 (MQ=255)                                                                                                                                                  ccTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCCTCCTGATGCCACTGTTCATGCttgggt                                                                                 >  2:68637/1‑233 (MQ=255)                                                                                                                                                     cgtaaatgagaggggggTAAAGGT‑AATGTGGCCCCGGCG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTGG‑CGATTG‑GTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCg                                                                               <  1:389605/224‑1 (MQ=255)                                                                                                                                                                               aGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCCTTATCAAGTTTTTCg                                                      >  2:197026/1‑239 (MQ=255)                                                                                                                                                                                  tGGACCCGACG‑CTGGGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTGTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGAt                                                    <  2:537292/238‑1 (MQ=255)                                                                                                                                                                                  gggACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TGAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGAt                                                    <  2:516265/237‑1 (MQ=255)                                                                                                                                                                                     aCCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAG‑TTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCt                                               >  1:4398/1‑239 (MQ=255)                                                                                                                                                                                      cccGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGAAACTGTTCATCCTGGGGTCGCTGGCGGGCATTTTCAAGtttttgcttccgcg                                               >  1:471592/1‑228 (MQ=255)                                                                                                                                                                                      cccGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTCGGG‑ACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAACGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAACGCTATATCGGTAAAA‑CTATTTTCACCACCATCCTGATGACACTGTTCAGTCTGTGGGCGTTGTCGGGCATTAACAAGTTTGTGCATCa                                                  >  1:335840/1‑236 (MQ=255)                                                                                                                                                                                                    gtttgtcccG‑TTA‑ACCTG‑TTTTTAT‑GTGCT‑TTAG‑GCATTG‑TTCCTAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCggg                                  <  1:466433/232‑1 (MQ=255)                                                                                                                                                                                                     tgtggaCCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCggg                                  <  2:71254/238‑1 (MQ=255)                                                                                                                                                                                                     tgtggaCCG‑TTA‑ACCTGATTTAGCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑GCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCggg                                  <  2:526978/238‑1 (MQ=255)                                                                                                                                                                                                      gtggcCCT‑TTA‑CCCTGGTTTTACT‑G‑GCT‑TTAT‑GCTGTG‑TTCGCAACCTTTGGG‑ACACCTGG‑CCGGTCCGCCGCCTGCG‑CGCCAGGTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAC‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCa                                <  1:807535/239‑1 (MQ=255)                                                                                                                                                                                                      gtggaCCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGc                                 <  2:88526/238‑1 (MQ=255)                                                                                                                                                                                                       tgtaCCT‑TTA‑ACCTTATTTTTCT‑G‑GCT‑TTGG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑GGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  1:873215/236‑1 (MQ=255)                                                                                                                                                                                                       tggtCCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TGAG‑CTATTG‑TTCTCAACCGTGGGG‑ACGCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTTATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCAGGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  2:809720/239‑1 (MQ=255)                                                                                                                                                                                                       tggaCCG‑TTA‑ACCTGATTTATCG‑G‑GCT‑TTAG‑CGATTG‑GTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  2:360120/239‑1 (MQ=255)                                                                                                                                                                                                       gggaCCG‑TTA‑ACCTGATTTATTG‑G‑GTT‑TAAG‑GGATTG‑TTCTCAACCTTGGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGGGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  2:191190/238‑1 (MQ=255)                                                                                                                                                                                                        ggaCCT‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  2:816240/238‑1 (MQ=255)                                                                                                                                                                                                        ggaCCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCCCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  2:181699/238‑1 (MQ=255)                                                                                                                                                                                                         tttataagagacaggtttttttt‑G‑GCT‑TTTG‑GTATTG‑TTCTCAACCTTGGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAg                               <  1:284154/217‑1 (MQ=255)                                                                                                                                                                                                         gaCCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAggg                             >  2:288181/1‑239 (MQ=255)                                                                                                                                                                                                         gaCCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGACgggggggg                             >  1:877743/1‑234 (MQ=255)                                                                                                                                                                                                           ccG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGGGTCGCGGTCTGGCATTATCAAGTTGTTCGATCAGCTGAAAAAAGCGGGGCAggggg                           >  1:648152/1‑238 (MQ=255)                                                                                                                                                                                                               ttA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGGGtt                        >  2:726723/1‑239 (MQ=255)                                                                                                                                                                                                                 a‑aCCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTac                      >  2:873517/1‑239 (MQ=255)                                                                                                                                                                                                                    ccTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTacga                    <  2:471592/239‑1 (MQ=255)                                                                                                                                                                                                                        aTTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAACAAGCCGGGCAGGGGAGttcgaacgcg                >  1:554900/1‑231 (MQ=255)                                                                                                                                                                                                                             tCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTAAGACGCGTTAg            >  2:90490/1‑238 (MQ=255)                                                                                                                                                                                                                               t‑G‑GCT‑TTAG‑CGTTTG‑TTCTCACCCTTGGGG‑CCCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  1:614621/239‑1 (MQ=255)                                                                                                                                                                                                                               t‑G‑GCT‑TTAG‑CGATTT‑TTCTCAACCTTTGGG‑ACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  2:194012/239‑1 (MQ=255)                                                                                                                                                                                                                               t‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  2:877743/239‑1 (MQ=255)                                                                                                                                                                                                                                gg‑gCT‑TTAG‑CTGTTG‑TTCCCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGgcg         <  1:125886/238‑1 (MQ=255)                                                                                                                                                                                                                                   gCT‑TTAG‑CGTTTG‑TTTTCCACCTGTGGG‑CTACCGGG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATAACGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  1:68637/239‑1 (MQ=255)                                                                                                                                                                                                                                   gCT‑TTAG‑CGATTG‑TTCACAACCTTTGGG‑ACCCCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  2:766119/239‑1 (MQ=255)                                                                                                                                                                                                                                   gCT‑TTAC‑GGATTG‑TTCTCAACCGTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  2:127658/239‑1 (MQ=255)                                                                                                                                                                                                                                    cT‑TTAG‑CGATTT‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  2:67913/238‑1 (MQ=255)                                                                                                                                                                                                                                    cT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTacg                     >  2:284154/1‑224 (MQ=255)                                                                                                                                                                                                                                    cT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAg      >  2:10380/1‑239 (MQ=255)                                                                                                                                                                                                                                     gcttttcgtaTTT‑TTCTCAACCTTTGGG‑ACCCCGTG‑CCGGTCCGGCCCCGGCC‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCATCCTTTTGGCGTACTTGCCCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCa       <  1:483033/230‑1 (MQ=255)                                                                                                                                                                                                                                       ttAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGGCCGCCGCCTTCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTGTCACCCCCATCATGATGACACTGTTCATGCTGGGGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGa    <  2:436852/239‑1 (MQ=255)                                                                                                                                                                                                                                        tAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCCGGaa   >  2:294853/1‑239 (MQ=255)                                                                                                                                                                                                                                         aG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGCAACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGCTCACCTGAAAAAAGCCGGGCAGGGGAGTTACGAACGGTTAGCGGCAGGAAt  >  1:221112/1‑239 (MQ=255)                                                                                                                                                                                                                                         aG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAACCCGGGCAGGGGAGTTACGACGCGTTTGGGGCGGGaaa  >  1:711360/1‑238 (MQ=255)                                                                                                                                                                                                                                          tggggttG‑TTCCCAACCTTTGGG‑ACACCGGG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGAAt  <  1:726723/234‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          ACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGA‑A‑CGGCGGTAAAGGT‑AAGCTGGACCCGACG‑CTGTGGATGTGGACCG‑TTA‑ACCTGATTTATCT‑G‑GCT‑TTAG‑CGATTG‑TTCTCAACCTTTGGG‑ACACCGTG‑CCGGTCCGCCGCCTGCG‑CGCCAGTTTTTCGC‑GTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAA‑CTATTTTCACCACCATCATGATGACACTGTTCATGCTGGTGTCGCTGTCGGGCATTATCAAGTTTGTCGATCAGCTGAAAAAAGCCGGGCAGGGGAGTTACGACGCGTTAGGCGCAGGAAT  >  NC_000913/4487018‑4487479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑