Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,509,878	A→C	15.1%	D84A (GAC→GCC)	yfeO →	putative ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,509,878	0	A	C	15.1%	151.1 / 4.3	73	D84A (GAC→GCC)	yfeO	putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base A (50/7);  new base C (2/14);  total (52/21)
Fisher's exact test for biased strand distribution p-value = 2.98e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGG  >  NC_000913/2509653‑2510109                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           tgttgtTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGACTTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGGTTTTTAAC‑GCAAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCA‑GGACCAGCAC‑CCGCCTGTGa                                                                                                                                                                                                                               >  2:354332/1‑238 (MQ=255) tgttgtTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGCATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGa                                                                                                                                                                                                                               >  2:377090/1‑238 (MQ=255) tgttgtTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTATGGGGATAGCCCAGGATT‑CACCCCACCGGATCATCGGTGTATTAAC‑GCTAACGGGAATTGCGGGGGGTTT‑GGTTTA‑CCGTTCTAG‑CAAGGGCA‑ATGCC‑AGACCAACCC‑CCGCCTGGGa                                                                                                                                                                                                                               >  1:276679/1‑238 (MQ=255)      ttattaTCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAAcc                                                                                                                                                                                                                            >  2:860410/1‑236 (MQ=255)        attatCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGCATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGCGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGGC‑ATGCT‑GCCCCCGACC‑CCGCCGTGGAACCGCTg                                                                                                                                                                                                                        >  2:833805/1‑238 (MQ=255)                tCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑TTGCC‑GGACCAGACC‑CCGCCTGTGAACCGTTGATCGgcgc                                                                                                                                                                                                                >  2:151668/1‑238 (MQ=255)                      ccgTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTAAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGGGAACCGCCGATCGGCGCCCCGGt                                                                                                                                                                                                          >  1:866728/1‑238 (MQ=255)                       cgTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGGGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGGCCAGACC‑CCGCCTGTGAACCCCCGACCGGCGAACCGGtt                                                                                                                                                                                                         >  2:613433/1‑238 (MQ=255)                          ggCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CAACCCTCTGGATCATCGGGAGATTAAC‑GCTAACGGGTATTCTGGTGGGGCT‑GGTTAT‑CCGTTTCAG‑CAAGGGAC‑CTTCG‑GGCTCAGACC‑CCCCCTGGGGACAGTGGAACGGCGCAcgcggtacg                                                                                                                                                                                                      >  1:691489/1‑230 (MQ=255)                             aaTTGGGATGTTGCCCCGTTTTGTTTTGTTTGTGGTGAACAAAATCCCCTGGGTATTACAGAATTTGCTCTGGCAACGACCGCCGGGACCTCTGGGGATAGCCCAGGATT‑CCCCCCGCTGGATCCTCGGTGTATTAAC‑GCTACCGGGTATTGCGTTGGGTTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGCCCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCct                                                                                                                                                                                                  <  1:799033/239‑1 (MQ=255)                                            cAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGCCCAGACC‑CCGCCTGTGAACCGCGGACCGGCGCACCGGTTTCGCCCTCTGCGCTACCTgg                                                                                                                                                                                     >  2:487390/1‑237 (MQ=255)                                                         aTTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCCtt                                                                                                                                                                      >  2:890954/1‑239 (MQ=255)                                                         aTTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCAtt                                                                                                                                                                      >  2:556668/1‑239 (MQ=255)                                                         aTTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTATGCGCTACCTACACTTATCTTAGCGtt                                                                                                                                                                      >  2:552572/1‑239 (MQ=255)                                                         aTTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGGGAACCGCTGATCGGGGCCCCGTGTCCGCCCTCCGCGCCCCCTGGAccttacggaacatt                                                                                                                                                                      >  1:474340/1‑226 (MQ=255)                                                         aTTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGGGAACCGCTGATCGGCGCACCGGTTCCGCCCCCGTCGCCACCTGGACTTTTGGGAGCAtt                                                                                                                                                                      >  1:695095/1‑239 (MQ=255)                                                         aTTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGGC‑ATGCC‑GGACCAGACC‑CCGCCGGTGAACCGCTGATCGGCGCACCGGTTCCGCCCCCTGCGCCACCTGGCCTTATCGGACCAtt                                                                                                                                                                      >  1:478089/1‑239 (MQ=255)                                                           tgtgGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑TTGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGGGCACCCGTTCCGCCCTCTCGCCAACCTGGCCtttctggtgcattaa                                                                                                                                                                    >  1:596035/1‑226 (MQ=255)                                                              ggtgATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCCCCGGTTCCGCCCTCTCCCCTACCTGGACTTATCGTAGCATTaaa                                                                                                                                                                   >  1:460169/1‑236 (MQ=255)                                                               gtgATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CGACCTGTGAACCGCTGACCGGGGCCCCGGGTCCGCCCTCCGCCCCACCTGGGCTTTACGTacacttatttt                                                                                                                                                                 >  1:27546/1‑228 (MQ=255)                                                                 gatgaAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTTTCGTAGCATTAATTCTCg                                                                                                                                                              >  2:224810/1‑239 (MQ=255)                                                                                          aaTTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTCCCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTggg                                                                                                                                     >  2:797454/1‑239 (MQ=255)                                                                                            tttGCTCTGGCAACGACTGCCGGGAGCTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGAATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTCCCTGGGCTTATCGTACACTTAATTTCCGGTCTTGCTGCGGGCGCCAcccggtgcc                                                                                                                                   >  1:401714/1‑233 (MQ=255)                                                                                            tttGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CTGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCCTTAATTCTCGGCCTTCCGTGCGGCGTACCCCTgggcc                                                                                                                                   >  1:536187/1‑237 (MQ=255)                                                                                            tttGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGcc                                                                                                                                                                                                    >  2:668708/1‑174 (MQ=255)                                                                                            tttGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTgggg                                                                                                                                    >  2:247245/1‑238 (MQ=255)                                                                                            tttGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATAGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCCGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCACTCTGCGCTACCTGGCCTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTAGGCCTtggg                                                                                                                                    >  2:438080/1‑238 (MQ=255)                                                                                            tttGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGACT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCACCCTCTGCGCTACCTGGACTTATCGGAGCCTTACTTCTCGGTCTTTCTGCGGGCGTAAGCCTGGGGc                                                                                                                                   >  1:313245/1‑239 (MQ=255)                                                                                            tttGCTCTGGAAACGACTGCAGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAAGGGGTATTGCGGGGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGc                                                                                                                                   <  2:478089/239‑1 (MQ=255)                                                                                               gctgtgccgaggcttGCCGGGACCTGGGGGGAGTGCCCATGTTT‑CCCCCCCGGGGTGCAGTGTTGTAGTAGC‑GCAAGCGGGTTGGGGGGTGGGTTG‑GGTTCG‑CCGTTTCGG‑CAAGGGTA‑ATGCC‑GGACCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGTCTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCgg                                                                                                                                <  1:506569/225‑1 (MQ=255)                                                                                               gCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATGAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCgg                                                                                                                                >  2:156488/1‑239 (MQ=255)                                                                                                    ggCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCTTCTGCGCTACCTGGACTTATCGTAg                                                                                                                                                                          >  2:60805/1‑192 (MQ=255)                                                                                                    ggCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCTTAGCATTAATTTTCGGTCTTGCTGTCGGCGTCAGCCTGTGCCCGGacaa                                                                                                                            >  2:524838/1‑235 (MQ=255)                                                                                                    ggCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACa                                                                                                                            >  2:10561/1‑238 (MQ=255)                                                                                                     gcaaggcggtccGGGAACTGTGGGGATACCCCGGGTTC‑CCCCCCTTGGGCTCAGCGGT‑TATCAACGGTTAAGGGGTTGGGGGGGGGGGTT‑GGTTAA‑CCGTCTCGG‑CAGGGGTC‑ATGCC‑GGCCCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACAt                                                                                                                           <  1:151668/228‑1 (MQ=255)                                                                                                             ttccGGGACCTTTGGGG‑TAGCCCCGGGTTCCCCCCCTCTGGTTCATGGTTGTATAAAC‑CTAAAGGGGTTTGGGGGTGGGGTT‑GGTTAT‑CCGTTCCAG‑CCGGGGTC‑ATGCC‑GGCCCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATg                                                                                                                  <  1:10561/237‑1 (MQ=255)                                                                                                                                tcccccgggttcccccccTCTGGATCTGCGGT‑TGTTAAC‑CGTTACGGGTTTTTGGGGGGGGTT‑TGTTTTCCCGTTCCAG‑CAGGGGCC‑AGCCC‑GGCCCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCt                                                                                                <  1:556668/224‑1 (MQ=255)                                                                                                                                 gCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCTTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATTGCCCtctg                                                                                             >  2:342087/1‑236 (MQ=255)                                                                                                                                 gCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGCCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGGCTTGCTGGGGGCGTCACCCCTGGGCCGGGCCACCCCATCactaccctacaaaacgccctttg                                                                                              >  1:790977/1‑217 (MQ=255)                                                                                                                                  cccAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTTCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCg                                                                                            >  2:408116/1‑239 (MQ=255)                                                                                                                                     aGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCCCGGCCTTGCTGGCGGCGTCAGCCTGCGGCCGGAACATCCGTTCATGACCGTCA‑ATATCGCCCTTGCGGtt                                                                                         >  2:481285/1‑239 (MQ=255)                                                                                                                                             aCCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCTTGACCTCCA‑ATATCGCCCTTGCGGTTGcacttg                                                                                  >  2:367785/1‑234 (MQ=255)                                                                                                                                             aCCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTg                                                                                  >  2:143763/1‑239 (MQ=255)                                                                                                                                             aCCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAACATTAATTCTCGGTCTTGCCGGCGGCCTCAGCCTGGGGCCGGAACCTCCGGCCAGAACCGTCACATGT‑GGCCTTGCGGTTGGCATTg                                                                                  >  1:84922/1‑239 (MQ=255)                                                                                                                                                   ctGGATCATGGGTGTATTAGC‑GCTAACGGGTATTGGGGTGGGTTT‑GGTTAT‑CCGTTTCAG‑CCGGGGTA‑ATGCG‑GGCCCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTc                                                                           <  2:474340/239‑1 (MQ=255)                                                                                                                                                      ggtccgcgtgtttatcgc‑GCTAGGGGTTATTGGGGTGGGGTG‑TGTT‑C‑CCGTCTCCGCCCAGGGCT‑ATGCG‑GGACCCGCCC‑CCGCCTTTGCACCGGTGATCGGAGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCTTTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGt                                                                         <  1:833805/221‑1 (MQ=255)                                                                                                                                                           tcGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGCTCCTGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCCCGCCTGtt                                                                    >  1:790481/1‑238 (MQ=255)                                                                                                                                                           tcGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTAGGGCCGGAACATCCGATCATGACCGGCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGtt                                                                    >  2:552726/1‑238 (MQ=255)                                                                                                                                                                gtATTAAC‑GCTAACGGGTATTGTGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTA‑ATGCC‑GGACCAGACC‑CCGCATGGGAACCGCTGATCGGCGCATCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGGAACATTCATTCTCGTGCTTGCTTGCGGCGCCAGGCGCGGGCCGGAACATCCGGTCATGACCGCAA‑CTATGGCCCCTGCTGTTGCATATGGC‑GCTCGTCTGTTAcccgc                                                              >  1:718568/1‑236 (MQ=255)                                                                                                                                                                gtATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGACCATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTTGC‑GCTCGTCTGTTACcgc                                                               >  2:366533/1‑238 (MQ=255)                                                                                                                                                                      aC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGGCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCCCGTCa                                                         >  2:395044/1‑238 (MQ=255)                                                                                                                                                                            aaCGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAGCATCCGATCATGACCGGCA‑CTATCGCCCTTGCGGTGTCCCTGGTC‑GCCCGTCTGTTAcgccccgcacaccgca                                                   >  1:557912/1‑224 (MQ=255)                                                                                                                                                                            aaCGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGc                                                                                       >  2:466861/1‑204 (MQ=255)                                                                                                                                                                              cGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATg                                                 >  2:449902/1‑239 (MQ=255)                                                                                                                                                                                 ttATGGCGGGGGGGTG‑TGTTTC‑CCGTTTCTG‑CC‑GGGTC‑CTTCCGGGCCCAGCCC‑CCTCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGa                                               <  2:105557/237‑1 (MQ=255)                                                                                                                                                                                 gttttGGGGTGGGGTT‑TGTTTC‑CCTTTTCGG‑CCGGGGTC‑CTGCC‑CGACCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTTCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCCGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGa                                               <  1:889275/235‑1 (MQ=255)                                                                                                                                                                                     tggggTGGGGTT‑TGTTAT‑CCGTTCACG‑CCAGGTTC‑AGTCC‑GGCCCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGa                                          <  2:313245/236‑1 (MQ=255)                                                                                                                                                                                     ggggggggggGT‑GTTTAC‑CGGTTCCAG‑CCAGGGTC‑AGGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGa                                          <  2:679222/233‑1 (MQ=255)                                                                                                                                                                                      gggggggggTTGGGTTTC‑CCGTTTCAG‑CCGGGTTC‑GGGCC‑GCACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGa                                          <  2:401714/234‑1 (MQ=255)                                                                                                                                                                                        gtgggggggtgttgttcccctttCCG‑CCCGGGTCGTTGCC‑GGCCCGCCCC‑CCGCCGTGGAACCGGTGTACGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGTAACATCCGAGCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTAGCGTGCGACAACCGAATGGAGTGGa                                          <  1:366533/219‑1 (MQ=255)                                                                                                                                                                                            gggTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGGTCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCTTTGTG‑GCCCGCCTTTTACCCCCCGTCAACCGAATGGGGGGGAAAAtttt                                   >  1:511829/1‑239 (MQ=255)                                                                                                                                                                                            gggTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑AAATCGCCCTTGCGGTTGCCCTTGAG‑CCTCGCCTGTTCCCGCGCGTCACCCACATGGAGTgggaaatttt                                   >  1:839146/1‑231 (MQ=255)                                                                                                                                                                                                             ttCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGCCTATTTTAGCCTCTGCCGGaa                     >  2:636645/1‑238 (MQ=255)                                                                                                                                                                                                                      gggTC‑ATGCC‑GGACCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGgcg            <  2:536187/239‑1 (MQ=255)                                                                                                                                                                                                                       ggTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGCGGCCGGATAATTCGCTCCTGCACGTCA‑CTGTCGCCCTTGCGGGTGCCATTGGCGGCACTTGT‑TTACCCGTCTGCAAACGCCGGGAGGGGACCATTTTTGCCTCTTCCGGAACCAACggtga           >  1:263442/1‑236 (MQ=255)                                                                                                                                                                                                                          tgaTGCC‑GGCCGAGCCC‑CCGCCTGGGACCCGCTGATCGGCCCACCGGGTCCGCCCTCTGCGCTCCCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCa          <  1:524838/236‑1 (MQ=255)                                                                                                                                                                                                                          tccggCC‑GGACCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCa          <  2:27546/234‑1 (MQ=255)                                                                                                                                                                                                                          tcagtcc‑GGACC‑GACCGCCGCCTGTGAACCGCTGATCGGCGCTCCGGTTCCGCCCTCTGCGCTACCTGGTCTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCa          <  2:26623/233‑1 (MQ=255)                                                                                                                                                                                                                          ccagcgc‑GGACCAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCa          <  2:596035/232‑1 (MQ=255)                                                                                                                                                                                                                             tGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCCTTGGC‑GCTCGTCTGTTACCGCGCGTCACCCGAATGGAGTGGACTATTTTAGCCTCTGCCGGaa                     >  2:395883/1‑224 (MQ=255)                                                                                                                                                                                                                                  ggcccAGCCC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTgg  <  2:460169/236‑1 (MQ=255)                                                                                                                                                                                                                                   gACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCa                                                         <  2:663067/183‑1 (MQ=255)                                                                                                                                                                                                                                   gACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCa                                                         >  1:663067/1‑183 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           TGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCTCTGGCAACGACTGCCGGGAACTCTGGGGATAGCCCAGGATT‑CACCCCTCTGGATCATCGGTGTATTAAC‑GCTAACGGGTATTGCGGTGGGGTT‑GGTTAT‑CCGTTTCAG‑CCAGGGTC‑ATGCC‑GGACCAGACC‑CCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCA‑ATATCGCCCTTGCGGTTGCCATTGGC‑GCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGG  >  NC_000913/2509653‑2510109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑