| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,619,521 | A→C | 13.5% | T134T (ACA→ACC) | marR → | transcriptional repressor of multiple antibiotic resistance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,619,521 | 0 | A | C | 13.5% | 197.5 / 2.9 | 82 | T134T (ACA→ACC) | marR | transcriptional repressor of multiple antibiotic resistance |
| Reads supporting (aligned to +/- strand): ref base A (39/28); new base C (5/8); total (44/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCG > NC_000913/1619284‑1619755 | ttcTCCGGTTGAACTGAAAAAGGTATTGGCGGTCGGCCTGGGAGCACTGACCCGTACGCGGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAacac < 1:475221/237‑1 (MQ=255) cGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCAATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGCCGAATTGGCAACACttacg > 1:725996/1‑236 (MQ=255) ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCCCTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTa < 1:910527/238‑1 (MQ=255) tGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAt < 1:215793/238‑1 (MQ=255) gaagctgggaaaGGTATTGCCGGTGGACCGGGGAGCACGGGCCCGGATTAGGGACAGCCTGGTCTGTAAAGGCTGTGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTAACACGGGCGGCGCGGCAATATGTGCACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAGAACCTGACGGCGGACGAAGTGGCAACACTTGAGTaa < 2:47841/229‑2 (MQ=255) gagcTGAAAAAGGTATTGTCGGCCGACATGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGCCCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt < 1:661714/235‑1 (MQ=255) aCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAttt > 1:81081/1‑237 (MQ=255) tGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGGATTTGCTTAAGAAAGTCCt > 1:926169/1‑238 (MQ=255) cggtcgACCGGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGcc < 1:829831/238‑1 (MQ=255) aacACTGACCGTTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAag < 1:125465/236‑1 (MQ=255) cTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCCA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGCCGCAGAAGCACCCCCTGAAGATTTTCTTAAAAAAGTCCCGCCCTAAAAAAAAAAAAGGTa > 2:683852/1‑239 (MQ=255) cTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAggaa > 1:451648/1‑237 (MQ=255) cTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGGGGCCACACTTCATGATTTTCTTAAAAAAATCCTGCCGAAAACAAAAAAAAggaa > 2:918693/1‑237 (MQ=255) aCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACCCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAACCAAAAAAAAGGAATg > 1:232223/1‑238 (MQ=255) aCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGAATTTGCTTAAGAAAGTCCTTCCGGCAAACAAAAAGAGGTAtca > 1:880968/1‑237 (MQ=255) gctgggctgtcTGGTTTGTAAAGGCTGGGTGG‑AAAGGTTGCCCAACCCGAATGACAAGCGGAGAGTACTGGTAAACCTTACCACGGGGGGCGCGGCAATATTGTAAAAATGCCATCAA‑TTAGTGGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACGCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTcc < 2:922165/229‑1 (MQ=255) gATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGAGGCAACCCTTGAGAATTTGCTTAAGAAAGTCCGGCCGTAAACAAAAAAAAgggaatcagatatccaaac > 2:708278/1‑222 (MQ=255) gATCGCCTGGTCTGTAAAGGCTGGGCGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTAATGGTAAAACTTAACACCGGCGGCGGGGCCATATGTGACCAATGCCATCAA‑TTAGTTGGCCCGGACCTCCACC‑‑‑AAGGATTAAAAAACAACCTGCAGGAGGGCAACGGGGAAACACCTTAGCATTTCTTTAAAAAAGACCTACCGGAAACAAAAAAAAggagtcacactttcacaac > 2:231882/1‑222 (MQ=255) gATCGCCTGGTCTGTAAAGACTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAGACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACCCAGAAGAATTAACAAAAAACCTGACGGCGGCCGCAGTGGCAAAACTTGAGGAATTGCTTAAAAAAATCCCGCCCTAACAAAAAAAAAAGGATGAGGAGGTCCa > 2:427058/1‑239 (MQ=255) tCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACg < 1:812716/238‑1 (MQ=255) ccTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCCGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAAt > 1:631821/1‑239 (MQ=255) ggTCTTGAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGGGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt < 1:1098761/239‑1 (MQ=255) ggTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAACCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt < 1:20345/239‑1 (MQ=255) gtttgggaaagtcgggTGGAAAAGTTTCCCGAACCCAAATAGCAACAGGGTGGTGATGGAAAATCTTACCACCGGCGGGGTAGCAATATTGGAAAAATGCCATCAA‑TTAGTGGGCCAGGACATGCGCC‑‑‑AAGAATTAACAAAAAACCTGACTGCGGACGAAGTGGCAACCCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGTTATGACGATGTCCAGACGCAATACt < 2:232223/226‑1 (MQ=255) gTCGGTAAAGGCTGGGGGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt < 1:562675/238‑1 (MQ=255) tgtacaggggggggggg‑AAAGGTCGCCGAACCCGAAGAAAAAGCGGGGGGGAGTGGAAAAACTTACCCCCGGGGGCGGGGAAATATGGGAACAATGCCATCAA‑TTAGTGGGCCAGGACCTGCACC‑‑‑AAGAATTAAAAAAAACCCGGACGGCGGACGAAGTGTCAACACTGGAGTATTTGCGTAAGAAAGACCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGa < 2:879011/227‑1 (MQ=255) cTGTAAAGGCGGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGGGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACCAAGAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTGGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt < 1:427058/239‑1 (MQ=255) aaaGGTGGGGGGG‑AAGGGTGGCCGAACCGGAAGTACAAGCGCGGCGTCTCGGTAAAACTTCCCACGGGCGGCGCGGAAAAATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATGTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTa < 2:572635/239‑1 (MQ=255) aaGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACCCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGAATGACCCTGTCCAGACGCAATACTGACGCTAt > 1:572635/1‑239 (MQ=255) ggCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTCCCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt < 1:809532/229‑1 (MQ=255) cgggTGA‑AAAGGTTCCCGAACCCTAATGAAAAGGGCGGCGTTCTTGCAAAACGTGCCGCCGGGGGGGCGGCAATATCGAAACAAGGCCACCAA‑TTAGTTTGACAGGTCCTTCACC‑‑‑AAGAAGTAACAAAAAACCTGACGGCGAACAAATTGGAAAAACTGGAGTATTTGCTTAAGAAAGTTCTGCCGTAAACAAAAAAGATGTATGACGATGTCCAGACGCAATACTGACGCTATTAcc < 2:541168/237‑1 (MQ=255) gggggg‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAATATTTGCTTAAGAAAGACCTGCCGTAAACAAAAAAGAGGAATGACGATGTCCAGACGCAATACTGACGCTATTACCAt > 1:801993/1‑239 (MQ=255) gggTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGAAGAACGATGTCCAGACGCAATCATGACGCTATTACCAt > 1:566194/1‑239 (MQ=255) gggTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGGGGCAACACTTGAGTATTTGCTTAAGAAAGGCCTGCCGTAAACAAAAAAAAGGAATGAAGAGTTCCAAAAGCaaaaacaacccaattaaaat > 2:726549/1‑221 (MQ=255) ggTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt < 1:1050214/224‑1 (MQ=255) gcgg‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATAAA‑TTAGTTGGCCAGGACCTGCACA‑‑‑AAGAATTAACAAAAACCATGACGGCGGACGAAGCGGCAACACTTGAATATTTTCTTACGAAAACCCGTCCGGAAACAAAAAAAAGGAAACAAGATGCCAAAACGCAAAAcacacaccaatacaaaaa > 2:517254/3‑222 (MQ=255) ggg‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTg < 1:570044/222‑1 (MQ=255) aagggTTGCGGAGCCCGAATGGCAAGCGCGGCGTCCTGTTAAAACTTACCCCGGGCGGCGCGGTAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATa < 1:405349/235‑1 (MQ=255) aaaGGTTGCCGACCCCGAATGAAAAGGGGGGGGTACTGGAAAAACTTACCCCCGGCGGGGCGGAAAAATGTGAAAAATGCCACAAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATa < 2:926169/238‑1 (MQ=255) aaaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACCCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAAAGGGATGAAGATGGCCAAACGCAATACTGAAGCTAATAcacttatctt > 2:809532/1‑230 (MQ=255) ttGCCGAACCCGAATGACAAGCGCGGCTTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAtt < 1:726549/238‑1 (MQ=255) ttGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGGGTATTTACTTAAGAAAATCCTTCCGGAAACAAAAAAACAGTAAGACCATGTCCAAACCCAAAACTGAAGccaatcactgacccttaaaaa > 2:1050214/1‑219 (MQ=255) aaCCGGAATTAAAAGCGGGGGGTACTTGTAAAACTTCCCCCCGGCGGCGCGGCAATATGGAAACAATGCCATCAA‑TTAGTTGGCCAGGCCCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACt < 1:683852/239‑1 (MQ=255) aCCCGAATGACAAGCGCGGCGTACTGGTAAAACTGACCCCCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACt < 1:132111/238‑1 (MQ=255) cGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTCCCATTCATAGCATTTTGGACTggta > 1:963123/1‑237 (MQ=255) aaaagggggcggttcTGGTAAAACTTCCCCCCGGGGGCGGGGCAATATGTAAAAAATGCCATCAA‑TTAGTGGGCCTGGCCCCGCACC‑‑‑AAGAATTAACAAAAACCCTGACGGCGGACGAAGTGGAAACCTTGGATTATTGGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGa < 2:859750/225‑1 (MQ=255) caagcgtgggttcgtGGTAAACCTTACCACCGGCGGCGCGGCAATATGTGAAAAAGGCCATCAA‑TTAGTTGGAACGGTCCTGGAAA‑‑‑AAGAATTAAAAAAAAAGATGAAGGAGGAAGAAGGGGAAAAAGGGGAGGAGGGGGTGAAGAAAGGAAGGAAGGAAAAAAAAAAGAGGGAGGAAGAGGTGAAGAAGAAATAGGGAAGATATTAAAATTAATAGAATTTTGGACTGGATAGAGGa < 1:231882/224‑1 (MQ=255) tAAAACTTCCCCCGGGGGGGGGGGAAATAT‑TGAAAAATTCCCACAATTTAGTGGGCCAGGACCTGCACA‑‑‑AAAAATTAAAAAAAACCCTGACGCCGACCGAAGTGGAAAACTTTGAGTATTTGCTTAAGAAGGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCAc < 2:451648/238‑1 (MQ=255) aaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGAATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCGGGAATCGcc > 1:68368/1‑234 (MQ=255) aCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGCATGACGAAGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGAAAAACTGGATTCGCCACTGTCa > 1:242198/1‑239 (MQ=255) tACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCCTTTTGGACTGGATCGGGGCCAACCTGGAATCGcccacttcactg > 1:459307/1‑229 (MQ=255) ccaccGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGTTGTCCAGACGAAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGCCAACCTGGAATCGCCACTGTCACTggg > 1:932907/1‑238 (MQ=255) gccGGCGGCGGGGCAATATGTAAAAAAGGCCATCAA‑TTAGGTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACCCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGgaga < 1:708278/238‑1 (MQ=255) gAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGtt > 1:527308/1‑239 (MQ=255) aaaaaaTGCCATAAA‑TTAGTTGGCCAGGACCTGCACA‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGt < 1:203755/234‑1 (MQ=255) aaCAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGGGAAAGTGTCAGAGCGTTCGTGtt > 1:208865/1‑238 (MQ=255) tGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACACAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAAACCCACCTGGCACTGGAAAAAAGGGCAGAAGGTTCGGGTAAATCCa > 2:608914/1‑239 (MQ=255) tGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGCTGTCCAGACGCAATACTGAAGCTATTACCATTCATAGCATTTTGGACTGGCTCCAGGACAACCTGGAATCCCCCCTGTCCCCGGGGAAAGGGTCACACGGTTCGGGttcaccca > 2:111443/1‑233 (MQ=255) tGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACg > 1:514583/1‑135 (MQ=255) tGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGgag > 1:256702/1‑211 (MQ=255) tGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGCAAGCGTACGAGCGGTCTGGtagttcca > 1:424161/1‑232 (MQ=255) tGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGGTACTCCa > 1:1004277/1‑239 (MQ=255) gCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCaa > 1:831363/1‑239 (MQ=255) a‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATAAAAAAAAAACCTGACGGCGGACGAAGGGGCAACCTTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCAc < 1:608914/239‑1 (MQ=255) taagggacAGGCCCTGCCCA‑‑‑AAGAATAAAAAAAAAACCGGCCGGCGGAGGAAGTGGCAACGTTGGAGTATTTGCTAAAGAAATTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTg < 2:3093/232‑1 (MQ=255) aGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCCGAGCGTTCGGGGTACTCCAAATGGCACCTGAAACGGAt > 1:474079/1‑239 (MQ=255) gCACC‑‑‑AAGAATTAACAAAACACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGAATTTTGGACTGGATCGAGGACAACCTTGAATCTCCACTGTCACTGGAGGAAATGTCAAAGCGTTCGGGTTACTCCAAATGGCCCCTGCAAAGGAGGTTaaaa > 2:1093605/1‑239 (MQ=255) gCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATa > 1:367106/1‑135 (MQ=255) gCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGa > 2:451030/1‑157 (MQ=255) gCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGa < 1:451030/157‑1 (MQ=255) gCACC‑‑‑AAGAATAAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATa < 2:367106/135‑1 (MQ=255) aaGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGTACCCTGCAACGGATGTTTAAAAAAGa > 1:18112/1‑239 (MQ=255) aGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCCAATGGCACCTGCAACGGATGTTTAAAAAAGaa > 1:942943/1‑239 (MQ=255) aGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGt > 1:3093/1‑228 (MQ=255) aGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGAATCGAGGACACCCTGGAAACGCCACTGTAACTGGAGAAAGTGTCAGAGCGTTCCGGTTACTCCAAATGGAACCTGCAAAGGAGGTTTAaaaaaaaa > 2:499963/1‑239 (MQ=255) tAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCgg > 1:575672/1‑239 (MQ=255) aCAAAAAACCTGACGGCGGACGAAGTGGAAAACATTGAGGTTTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGtc < 1:1060092/239‑1 (MQ=255) cAAAAACCCGGACGGGGGAGAAGGTGAAAAGATTTGAGTTTTTGCTAAAGAAAGCCCTGCCGTAAACAAAAAAGAGGTATGACTATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGtca < 2:527308/239‑1 (MQ=255) aaaaaccccTGGCGGCGGACGAAGGGACAACATGTGGTTTTTTGCATAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGtca < 2:459307/232‑1 (MQ=255) gACGGCGGACGAAGTGGCAACCCTTGAGTTTTTGCTTAAGAAGGTCCTGCCGTAAACAAAAAAGAGGTAGGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGGCTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGc < 1:800383/239‑1 (MQ=255) cggcggACAAATGGGCAACCTTTGAATATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATTACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGcc < 1:431458/238‑1 (MQ=255) cggACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGGCTGGATCGAGGACAACCTGGAATCGCCCCTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCCAATGGCACCTGCAACGGATGTTTAAAAAAAAAACCGGGCCTTCCTTAGGCCAATa > 2:800383/1‑239 (MQ=255) cAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCg > 1:65015/1‑239 (MQ=255) | TACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGG‑AAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAA‑TTAGTTGGCCAGGACCTGCACC‑‑‑AAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCAGAGCGTTCGGGTTACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCG > NC_000913/1619284‑1619755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |