BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,935,990	T→C	11.7%	S137P (TCC→CCC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,990	0	T	C	11.7%	293.2 / 3.9	115	S137P (TCC→CCC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base T (66/31); new base C (2/14); total (68/47)
Fisher's exact test for biased strand distribution p-value = 3.65e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCG  >  NC_000913/2935752‑2936228

tgccgaagccggTATCGCGGGGTGGGTTGAACGCCCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTAAGGTAACGCCTGGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCACCCCGCCCGAAGGCCATGTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATgct                                                                                                                                                                                                                                                 <  2:952356/228‑1 (MQ=255)

    gATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCAt                                                                                                                                                                                                                                             >  1:125792/1‑239 (MQ=255)

       cccTGTATCGGGGTTATGGCCGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACAATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTa                                                                                                                                                                                                                                          <  1:820161/238‑1 (MQ=255)

            tATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCGGACAGGCATCCCAGCATTCTCCATTTACGGTc                                                                                                                                                                                                                                     >  1:209981/1‑239 (MQ=255)

            tATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTc                                                                                                                                                                                                                                     >  1:93035/1‑239 (MQ=255)

            tATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CCCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTc                                                                                                                                                                                                                                     >  1:460971/1‑239 (MQ=255)

             aTCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCCTTCCGGTAACGCCTTGCTGGTACTATGGCAGTGAAACCATCGAACTGGACCCAACCCGGCCGAAGGCCATTTGGGGCTTTAACCGCACTGAACGCCCCGGCCCTGTTTAACCTGCAGCGGCCCCTGT‑CA‑CACCCAGCCCAAAAGCCCCCCCACCATTCTCCaatagcgggca                                                                                                                                                                                                                                    >  2:903181/1‑229 (MQ=255)

               cgcgGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                                                                                                                                                                                                                                  <  1:693131/239‑1 (MQ=255)

                  gggTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACg                                                                                                                                                                                                                               <  1:803092/239‑1 (MQ=255)

                      aTGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCa                                                                                                                                                                                                                           >  1:1085221/1‑239 (MQ=255)

                              agctcctgctTGCGAAAAAAAATTCGTCAGTCGGTATGTAGGCCTCCACATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGcc                                                                                                                                                                                                                    <  2:121766/233‑1 (MQ=255)

                                  tctgctTGGCAAGAAAAAGTCAGGAGGCAGAATGGAGGGCACACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGa                                                                                                                                                                                                               <  2:125792/238‑1 (MQ=255)

                                  gctgctTGCGAAGAAAAATTCAGCAGTCAGAATTTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGa                                                                                                                                                                                                               <  1:342825/239‑1 (MQ=255)

                                  cctgctTGTGAAAAAAAATTCAGGAGGCGGAATTTGGGCCCCACCATTCCGGTCACGCCTTGGTGGTGCTCTGGCAGTGAAACCCTCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGa                                                                                                                                                                                                               <  2:896512/238‑1 (MQ=255)

                                        tacGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATc                                                                                                                                                                                                         >  1:206486/3‑239 (MQ=255)

                                                  aaTTCATCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCGGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTCCCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGcc                                                                                                                                                                                               <  1:770752/239‑1 (MQ=255)

                                                                aaggtgggCCTCCCCATTACGGTAACGCCTTGCTGGTGCTATGGAAGGGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTTTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                                                                                                                                                                                                                             <  2:474037/189‑1 (MQ=255)

                                                                     tggCCTCACATTTCCGGGACAGCCTTGCTGGGCGTAGGGCAGTGAAACCATCGGCATGGACCCAACCCGCCGGAAGGCCCTTTGGGGGTTTAACGGCACTGAGCGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAActgc                                                                                                                                                                            <  2:209981/238‑1 (MQ=255)

                                                                      ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGCTGTTGAAGAAAAAct                                                                                                                                                                              >  1:278502/1‑236 (MQ=255)

                                                                          tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTCCGGTCATGACGTTCAGGAGGCCGAACACACCACGATTCCTGCCCAAGTTAAAAAAAAAACGCTccg‑c                                                                                                                                                                      >  2:55147/1‑239 (MQ=255)

                                                                          tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACtgctgc                                                                                                                                                                         >  1:733819/1‑237 (MQ=255)

                                                                          tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACtgctgc                                                                                                                                                                         >  1:147652/1‑237 (MQ=255)

                                                                          tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACtgctgc                                                                                                                                                                         >  1:464294/1‑237 (MQ=255)

                                                                          tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAAACATCGATTCCTGCCGATGTTGAAGAAAAACtgctgc                                                                                                                                                                         >  1:880045/1‑237 (MQ=255)

                                                                            aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGGAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCAAAGCCAGAAAGGCATCCCAGCCTTACCCATTTACGGTCCTGCCCTTCAGGATACCGATGACACATCGACTCACCCCCATGTTGAAAAAAAACGGCTGCG‑Ctt                                                                                                                                                                    >  2:482751/1‑239 (MQ=255)

                                                                            aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCAt                                                                                                                                                                                                                                   <  2:450377/177‑1 (MQ=255)

                                                                            aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCAt                                                                                                                                                                                                                                   >  1:450377/1‑177 (MQ=255)

                                                                            aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGACGAAAAACTGCTGCG‑Ctt                                                                                                                                                                    >  1:2337/1‑239 (MQ=255)

                                                                            aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGAAGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAAAAAAACtgcggccatt                                                                                                                                                                     >  2:931022/1‑235 (MQ=255)

                                                                                 tACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCa                                                                                                                                                                                                                                    <  2:1015598/171‑1 (MQ=255)

                                                                                 tACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCa                                                                                                                                                                                                                                    >  1:1015598/1‑171 (MQ=255)

                                                                                 tACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGcc                                                                                                                                                                >  1:117232/1‑238 (MQ=255)

                                                                                      tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCCCAGCCAGAAAGCCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACCCATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGcc                                                                                                                                                          >  2:1098319/1‑239 (MQ=255)

                                                                                        aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATTTTGAGAAAAAACTGCTGCT‑CTTTGCCCCCGCCgg                                                                                                                                                        >  1:402032/1‑239 (MQ=255)

                                                                                        aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGCAAAACTGCTGCG‑CTTTGCCCGCGCCgg                                                                                                                                                        >  1:549507/1‑239 (MQ=255)

                                                                                        aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCCTTGACCCACCGATCCCTTCCGATGTGGAAGAAAAATTCCGCCG‑CTTTGCCCCCCCCgg                                                                                                                                                        >  2:390521/1‑239 (MQ=255)

                                                                                        aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAAGACGCCGAAGACCCCTCGATCCCTGCCGAGGTTGAAGAAAAActcgcgccctttacacccccagg                                                                                                                                                         >  2:117863/1‑218 (MQ=255)

                                                                                        aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCCCCATTTACGGCCATGACGTTCAGGATGCCGATTACCCATCCATTCCTGCCGATTTTGAAGAAAAAATGCTGGC‑CTTTGCCCGCCCCgg                                                                                                                                                        >  2:292824/1‑239 (MQ=255)

                                                                                              tGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGCTGTTGAAGAAAAACTGCTGCG‑CTTTGCCCCGCCCGGTTTgg                                                                                                                                                   >  1:1067625/1‑238 (MQ=255)

                                                                                                    tGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCg                                                                                                                                             >  1:542171/1‑238 (MQ=255)

                                                                                                    tGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCCCGCCGGTTTGGCTGTCg                                                                                                                                             >  1:32679/1‑238 (MQ=255)

                                                                                                     gCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGc                                                                                                                                            >  1:119789/1‑238 (MQ=255)

                                                                                                     gCTATGGCAGTGAAACCATCGACATGGATCCAACACGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTGCCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAACCTTATCCCTTTACGGTCATGACGGTCAGGATGCCTATGCCAACACGATCCCTGCCGAGGTTGATGCAAAAATGCTGCG‑CTTTTCTCGCGCCGGTTTGGCcgccgca                                                                                                                                           >  2:817323/1‑238 (MQ=255)

                                                                                                             aGTGAAACCATCGACCGGGATCCAACCCGCCCGAAGGCCATGTGGGGCTTTAACGGCACTGAACCCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTGCGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGa                                                                                                                                    <  1:398986/238‑1 (MQ=255)

                                                                                                              gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGc                                                                                                                                                                 >  1:83476/1‑208 (MQ=255)

                                                                                                               tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCCTGAAAg                                                                                                                                 >  1:1008773/1‑239 (MQ=255)

                                                                                                               tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCC‑CTGACACATCGATTCCTGCCGCTGTTGAATAAAAACTCCTCAG‑CTTTGCCCGCGCCGGCTTGGCCTTCGCCAGCCTGAACgg                                                                                                                                >  1:1048037/1‑239 (MQ=255)

                                                                                                                   aCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CCGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGGTGAAGAAAAACTGCTGCG‑CTTTCCCCCTCCAGGTTTGGCCGTCGCCAGCAGGAAAGGGaa                                                                                                                             >  2:162693/1‑239 (MQ=255)

                                                                                                                     cATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTaaaa                                                                                                                           <  1:89576/239‑1 (MQ=255)

                                                                                                                      ttCGACAGGGGTCCAACCCGGCAGAAGGCATTTGGGGGCTTTAACGGCACTGATCGCCCCGGTGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGCTGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAg                                                                                                                          <  1:903181/238‑1 (MQ=255)

                                                                                                                                 cccACCCCGCCCTAAGTCCTTTGGGGGTTTTAACGGCCCTGAACCCCCCGGGGCTGTTTCCCGGGCAGCGG‑TTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCg                                                                                                                <  2:542171/237‑1 (MQ=255)

                                                                                                                                     aCCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCg                                                                                                                                                                                              <  1:805724/157‑1 (MQ=255)

                                                                                                                                     aCCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGCTGAAGAAAAACTGCTGCG‑CTTTTCCCCCGCCGGTTTGGCCGTCGCCAGCATAAAAGGAAAAAGCCATCTGTCGCTggg                                                                                                           >  2:533109/1‑239 (MQ=255)

                                                                                                                                       ccccccgAAGGCCTTTTGGGGTTTTAACGGCACTGAACGCCCCGGGGCTGTTTACCTGGGAGGGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGggcg                                                                                                         <  2:147652/236‑1 (MQ=255)

                                                                                                                                          cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCCCAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGCCGTTCAGGATGCCGTTGACACATCGATTCCTGCCGTTGTTGAAGAAAAACGGC‑GCGCCTTTGCCCCCGCCCGTTGGCACTGCGCCGGCATAAAAGGAaaaacccaccgtccgcggggggggg                                                                                                      >  2:588115/1‑218 (MQ=255)

                                                                                                                                          cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACCGCCAGAAAGGCATACCAGCATTCTCCATTTACGGTCCTGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGCTGTTGAAGCAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGGCGCCAGACCGAAAGGGaaaaccattctttgcccgggggggg                                                                                                      >  2:989432/1‑218 (MQ=255)

                                                                                                                                          cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCCCTGGgcggcg                                                                                                      >  1:282202/1‑239 (MQ=255)

                                                                                                                                                     tttGGTGCTTAAAGGGCCTTAGACCCCCCGGCGCTGTTTGCCTGGCCGCGG‑‑TCTGGCCCGTCCACGCGCAGAAAGGCATCCCAGCATTCTCTATTTACGGTAATGACGTTCAGGATGCCGATGCCCCATCGATTCCTGCCGGTGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGTCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGTCGTTGCGATGGGt                                                                                           <  2:840705/239‑1 (MQ=255)

                                                                                                                                                     tttGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGCTTCCTGCCGATGTTGAAGAAAAACTGCGCCT‑CTTTGCCCCCGCCGGTTTGGCCGTCCCCGCCATCAAAGGGAAAAGCTATCTGTCCCGGGGGGGGGTTGCGCTggag                                                                                           >  2:219824/1‑237 (MQ=255)

                                                                                                                                                     tttGGGCCCTTACCGGCCGTGCACGCCCCGGGGCTGTTTACCGGGCTGCGG‑CTCTGG‑CAGCTCACAGCCGGAAAGGCATCCAAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACCCGTCCGTTCCTGCCGATGTTGAAGAAAAACGGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGt                                                                                           <  2:880045/239‑1 (MQ=255)

                                                                                                                                                          gggtttacctgcagcgAACGCCCGGCGGGTGTTTCCCGGGCGGCGT‑GTCTGG‑CATACCACAGCCAGTAAGGCAGCCCAGCATTTCCCATTTACGGGTTGGTCGTCAAGGA‑GTCCAGGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCTCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGGCGCTGGGCGGCGTTTCGATGGGTATCGc                                                                                      <  2:1048037/223‑1 (MQ=255)

                                                                                                                                                               tAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTTCCCGCGCCGGTTTGGCCGTCGCCCGCATGAAAGGTAAAAGCAATCTTTCCCTGGGCGGCGTTTCGTGGGGATTCGCCGGt                                                                                  >  2:647609/1‑238 (MQ=255)

                                                                                                                                                                aaCGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTTCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAACGATTCTGTCGCTGGGCGGCGTTTCGATGGGGATCGCCGGTTc                                                                                >  2:165032/1‑239 (MQ=255)

                                                                                                                                                                  cGGCACTGACCGCCCCGGCGGTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATCCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCa                                                                              <  1:165032/239‑1 (MQ=255)

                                                                                                                                                                      ccgtacccccccGGCGGTTTTTCCCGGGGAGGGG‑CTCGGG‑CAGCTCCAAGCCAGAAAGGCCTCCCAGGATTTCCCATTTACGGTCATGGCGTTCAGGATGCCGGTGACACGTCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCAttg                                                                           <  2:235301/230‑1 (MQ=255)

                                                                                                                                                                       ccgcaccccccgggtttttttACCTGGGGGCGG‑GTCTGG‑CACGTCCAAGCAAGAAGGGCATCCAAGCATTCGCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCAttgtt                                                                         <  2:687403/221‑1 (MQ=255)

                                                                                                                                                                          acccccccGGCGTTTTTTTCTTGGCAGCGT‑GTCTGG‑CTCCTCCCAGCCAGAAGGGCATCCCATCATTCCCCATTTACGGTCATGAGGTTAAGGATGCCGATGCCACATGGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGAt                                                                      <  2:460971/235‑1 (MQ=255)

                                                                                                                                                                          aaCGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGa                                                                       >  1:350454/1‑238 (MQ=255)

                                                                                                                                                                           accccccgtgcctttttACCTGGCAGGGG‑CTCTGG‑CCGCCCAAAGCGAAAAAGGCCTCCCAGCATTCCCCATTTCCGGTCATGACTTTCAGGATGCCGAGGACCCACCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATc                                                                     <  2:1085221/225‑1 (MQ=255)

                                                                                                                                                                               cccGGGGCTTTTTACCTGGCAGGGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCCCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACaa                                                                 <  1:533109/239‑1 (MQ=255)

                                                                                                                                                                               cccGGCGGTTTTTCCCTGGTCGGTG‑CTTTGG‑CAGCTCACAGCCAGAAAGGCCTCCCAGCATTCCCCATTTACGGTCATGACGTTCAGGATGCCGATGACCCATCGTTTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACaa                                                                 <  2:93035/239‑1 (MQ=255)

                                                                                                                                                                               cccGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAACGGTAAAAGCTATCTGTCGCGGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATcaca                                                                  >  1:114024/1‑238 (MQ=255)

                                                                                                                                                                               cccGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATccca                                                                  >  1:235301/1‑238 (MQ=255)

                                                                                                                                                                               cccGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGATGCG‑CTTTGCCCGCGCCGGTTTGGCCCTCGCCAGCATGAACGG‑GAAAGCTATCTGCCGCTGGGCGGCGTTTCGGAGGGTAAGGCGGGGTCCATTGTTGATAACaaa                                                                >  2:552799/1‑238 (MQ=255)

                                                                                                                                                                               cccGGCCCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCgg                                                                                   <  1:658267/221‑1 (MQ=255)

                                                                                                                                                                                         tttACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGa                                                        >  1:446208/1‑238 (MQ=255)

                                                                                                                                                                                         tttACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGAAAAAGCTATCTGTCGCTGGGCGGCGTTTCGCGGGGGATCGCCGGTTCCAttgttgctgtctcttatacac                                                         >  2:658267/1‑222 (MQ=255)

                                                                                                                                                                                          ttACCTGGCCGCGG‑CTTTGG‑CGTCACCCAGACAGAAAGGCAACCCAGCATTCCCCATTGACGGTTGAGACGTTGAGGATGCCGATGACACATGGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCTGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAAt                                                      <  2:32679/239‑1 (MQ=255)

                                                                                                                                                                                                 cggccggggttctggcgcatCACAGACCGAAAGGCATCCCAATATTTCCCATTTACGGTCATGAAGTTAAGGATGCGCATGACCCCCCGATCCCTGCCGAGGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCctgg                                                 <  2:774517/220‑1 (MQ=255)

                                                                                                                                                                                                   aGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTTGCGCTGGGCGGCGTTTCGAAGGGCACCGCCGCTTTCATTGTTTGTCACAACTTCTTTGTATCctggttgg                                             >  1:597069/1‑239 (MQ=255)

                                                                                                                                                                                                     ccggttttGG‑AAGCTCACACCCCAAAAGGCATCCCAGCATTCCCCTTTTACGGTCATGACGTTCAGGATGCCGATGACCCATCGTTTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTggg                                            <  2:1008773/232‑1 (MQ=255)

                                                                                                                                                                                                     cGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCCTTGTTGATCACAACTTCTTTGAATCCTGGCTgggg                                           >  1:1035865/1‑238 (MQ=255)

                                                                                                                                                                                                            tGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCGCCATTTTCGGTCATGACGTTCAGGATGCCGATGCCACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGaaa                                     <  1:931022/239‑1 (MQ=255)

                                                                                                                                                                                                                    taacaGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTTCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGGAAAAGCTATCTGTCGCTGGGCGGCGTTTGCAGGGGGATCGCCGGTTCCCTTGTTGATCACACCTGCTTTGAATCCGGGCTGGGATGTAAAGTCAAgg                              >  2:927915/3‑239 (MQ=255)

                                                                                                                                                                                                                    ccccgcccAAAAAGGCTACCCCGTTTTTTCCATTGACGGTCATGGCTTTCCGGCGGCCGATGACCCATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAgg                              <  2:1092544/233‑1 (MQ=255)

                                                                                                                                                                                                                      acaGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGTTGGGTATCGCCGGTTCCCTTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCg                            >  1:111029/1‑239 (MQ=255)

                                                                                                                                                                                                                      aaaGCCAGAAAGGCCTCCCAGTATTTCCCAGTTACGGTCATAACGGTCAGGATGCCGATGACCCATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAAGCCTGGCTGGGAATGAAAGTCCAGGCg                            <  1:647159/237‑1 (MQ=255)

                                                                                                                                                                                                                        aGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGc                                                                                      <  1:310013/179‑1 (MQ=255)

                                                                                                                                                                                                                        aGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGc                                                                                      >  2:310013/1‑179 (MQ=255)

                                                                                                                                                                                                                        aGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCg                            <  1:840528/237‑1 (MQ=255)

                                                                                                                                                                                                                          cccgAAAGGCCTCCACGCATTCCCCTTTTACGGTAGTGACGTTAGGGATGCCGATGACACATAGATCCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGGCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCg                            <  2:687391/232‑1 (MQ=255)

                                                                                                                                                                                                                          ccAGAAAGGCCACCCAGCATTTCCCATTTACGGTAATGCCGGTCAGGATGCCGATGACCCCTCGTTTCCTGCCGAGGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCg                            <  2:710596/235‑1 (MQ=255)

                                                                                                                                                                                                                             gAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTGTAGCCGGTTCCATTGTTGTTCACACCTTCTTTGATTCCTGGTCGGGAATGAAAGTCCAGGGGGGGGgta                     >  2:67550/1‑239 (MQ=255)

                                                                                                                                                                                                                                aGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAGCGTTTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGa                  <  1:636552/239‑1 (MQ=255)

                                                                                                                                                                                                                                aGGCATCCCAGCATTCCCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGa                  <  1:656350/239‑1 (MQ=255)

                                                                                                                                                                                                                                  gCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGAAAAAGCTATCTGTCGCTGGGCGGCGTTTCGACGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGAGGGGACTGAAAGTCCGGGGGGGGGAAATTAcc                >  2:840528/1‑239 (MQ=255)

                                                                                                                                                                                                                                    aTCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGTTATGAcccg              >  1:687391/1‑237 (MQ=255)

                                                                                                                                                                                                                                    aTCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGa                  >  1:710596/1‑235 (MQ=255)

                                                                                                                                                                                                                                    aTCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGAAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCCCCGGTCCCATTGTTGACCCCAACGTCTTTGAAACCTGGCTGGGAATAAAAATCAAGGAGGTGGACA‑GACCGaa             >  2:494727/1‑239 (MQ=255)

                                                                                                                                                                                                                                                tcCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGCGGGATATGACCGAACTGCGtgtccg  >  1:116211/1‑234 (MQ=255)

TTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGG‑CTCTGG‑CAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCG‑CTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCG  >  NC_000913/2935752‑2936228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`