Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	111,197	C→G	14.4%	R52G (CGT→GGT)	mutT →	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,197	0	C	G	14.4%	210.7 / 2.7	88	R52G (CGT→GGT)	mutT	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (10/62);  new base G (13/2);  total (24/64)
Fisher's exact test for biased strand distribution p-value = 8.96e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGG  >  NC_000913/110963‑111434                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                   gTGCTATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTAGTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑Tg                                                                                                                                                                                                                                                                <  1:219299/238‑1 (MQ=255)   gcaagggCCGTCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGAAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑Tg                                                                                                                                                                                                                                                                <  2:646914/231‑1 (MQ=255)   gCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑Tg                                                                                                                                                                                                                                                                <  1:707354/236‑1 (MQ=255)   gCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGCAAAATTGCAATGGGTGAAACGCCGAGACA‑G‑GACGG‑T‑GGG‑GCG‑GG‑ag                                                                                                                                                                                                                                                             >  1:19962/1‑238 (MQ=255)   gCCAGGGCCGCCTGCAATAAAAGGTAACTGTTGAAGTAAAAGGCGCAGGGTTTTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTAGTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCCCATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑a                                                                                                                                                                                                                                                              <  2:360845/237‑1 (MQ=255)           cgccTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGga                                                                                                                                                                                                                                                    <  1:1236977/238‑1 (MQ=255)              cTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGGGAAACGCCGGAACA‑G‑G‑CGG‑G‑GGT‑GGG‑TG‑GAC‑TTTCGgaaa‑aa                                                                                                                                                                                                                                               >  1:978736/1‑239 (MQ=255)                gCAATAAAAGCTAACTGTTGAAGGAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑t                                                                                                                                                                                                                                            <  1:62013/239‑1 (MQ=255)                gCAAAAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑t                                                                                                                                                                                                                                            <  1:804134/239‑1 (MQ=255)                        aacTAACTGTTGAAGTAAAAGGCGCAGGTTTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTAGTATTCGCACCGAGAACAATGAAATCTTTATACCGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGCAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTa                                                                                                                                                                                                                                 <  2:958330/237‑1 (MQ=255)                           tAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGGGAAACGCCGGAACAGG‑G‑GGG‑T‑GGG‑GGG‑GG‑AAC‑TTGTGGAAG‑AAA‑G‑C‑gggggtac                                                                                                                                                                                                                                 >  2:959596/1‑233 (MQ=255)                                         aaaGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑G‑GGG‑GCG‑GG‑AAA‑TTCCGGGAGAAAA‑C‑C‑GGGA‑TAACCCCCCA‑AA‑A‑ttttc                                                                                                                                                                                                               >  1:181900/1‑237 (MQ=255)                                                    aTTCTTGGCTTTTTTTATTGGTTTAAGAAAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGATAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGaaa                                                                                                                                                                                                <  2:181900/238‑1 (MQ=255)                                                        tGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGGGAAACCCCGGAAAA‑GCG‑GGG‑G‑GGG‑GGG‑GG‑CAT‑TTC‑GGGAG‑AGA‑A‑C‑GGGG‑ATACCCCCCC‑CC‑A‑ATTTTTG‑C‑TA‑TTTTaaaaaaaa                                                                                                                                                                                           >  2:62903/1‑236 (MQ=255)                                                        tGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGGAAAATTGTAATGGGGGAAACGCCGGAACA‑G‑G‑CGG‑G‑GGT‑GGG‑GT‑AGA‑TTTAGGAAG‑AGG‑TGG‑GGGG‑TTACCCCCCA‑AC‑‑‑TTTTTTT‑C‑GA‑TTTGaaaaaaaa                                                                                                                                                                                           >  2:748808/1‑236 (MQ=255)                                                             cTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAAACCCGGAAAA‑G‑G‑GCG‑T‑GGG‑GGG‑GGTAAC‑CAC‑GGAGG‑AAG‑A‑CGGGGG‑TAACCCCCCA‑AC‑A‑tttttgtgttttttaaaaaagggaaa                                                                                                                                                                                          >  2:1184427/1‑218 (MQ=255)                                                             cTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGGGAAACGCCGGAACA‑G‑G‑CGG‑A‑GGTGGCG‑GC‑AAC‑TTAGGAAAG‑AAG‑T‑G‑GGGA‑TTACCCCCCC‑AC‑A‑TTTTTCC‑C‑TT‑TTTGAAAAAC‑GGGaaa                                                                                                                                                                                      >  1:679696/1‑238 (MQ=255)                                                               tttttATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACCCCGGAACA‑G‑G‑AGG‑C‑GCG‑GGG‑GG‑CACATCCAGAAAA‑AAG‑A‑C‑GGGA‑TCACCCCCC‑‑AC‑A‑ATTTTCGTC‑TT‑TTTATAAAAA‑TGGaaaaa                                                                                                                                                                                    >  2:304443/1‑236 (MQ=255)                                                               tttttATAGGTTTAAAACAAAGAAAAAGCGTCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGGCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATg                                                                                                                                                                                   <  2:650895/239‑1 (MQ=255)                                                                  ttATAGGTTTAAGAAAAAGAAAAAGCTGCAAATTGGGGTGGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AAt                                                                                                                                                                               <  2:19962/239‑1 (MQ=255)                                                                       ggTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑C‑GGT‑GCG‑TG‑AAC‑TTCAGGGAG‑AAA‑T‑C‑GGGG‑GTAACCCCCA‑AA‑A‑TTTTTTG‑C‑TA‑TTTTAAAAAC‑ATGCAAATG‑AATTACCg                                                                                                                                                                          >  1:555138/1‑239 (MQ=255)                                                                        gTTTAAGACAATGAAAAAGCTGCAAAGTTCGGTGGGTTTTTTTCGCAAAGAGAACAATGAAATTGTTATAACGCGTGGCGAAGAGGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCgg                                                                                                                                                                         <  2:34886/239‑1 (MQ=255)                                                                         tttAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTc                                                                                                                                                                             <  1:31153/234‑1 (MQ=255)                                                                         tttAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCg                                                                                                                                                                          <  1:309726/237‑1 (MQ=255)                                                                          ttaaagcAACGAAAAAGCTGCAAATTGCGGTTGGTATTATTCGCACCGAGAACAAGGAAATCTTTATAACGCGTCGGGCAGCAGATGCCCACATGGCCAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTCCCCCCCA‑AC‑A‑TTTCTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  2:330947/233‑1 (MQ=255)                                                                          ttaaacaaaggAAAAAGGTGCAAATTGGGGTAGGTATTAATCGCAACGAGAACAATGAAATCTTTATTACGCGTCGCGCAGCAGATGCGCACATGGGGAATAAACTGGAGTTTCCCGGCGGTAAAAATGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑TCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  2:180466/229‑1 (MQ=255)                                                                          ttaaacaaaagAAAAAGCTTCAAATTGCGGTTGGGTTTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGAGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑GTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  2:420210/229‑1 (MQ=255)                                                                          ttAAGACAATGAAAAGGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  1:946062/239‑1 (MQ=255)                                                                          ttAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  1:736956/239‑1 (MQ=255)                                                                          ttAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTAAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  1:438252/239‑1 (MQ=255)                                                                          ttAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑CTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  2:551392/239‑1 (MQ=255)                                                                          ttAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTAGAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGa                                                                                                                                                                        <  1:1187866/238‑1 (MQ=255)                                                                          ttAAGACAATGAAAAAGCTGCAAATGGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  1:727375/239‑1 (MQ=255)                                                                          tgaaGCCATTGTAAAAGCTTCAATTTGTGGTAGGGATTATGCGCAACGAGTACAATGAAATCTTTATGACGCGGCGAGCAGGAGATGGGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  2:124215/237‑1 (MQ=255)                                                                           tAAGGCAATGAAAAAGGTGAAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTAAAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  1:457318/238‑1 (MQ=255)                                                                           tAAGGCAATGAAAAAGGTGAAAATTGCGGTAGGTAATTATCGGAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAc                                                                                                                                                                       <  1:275663/238‑1 (MQ=255)                                                                           tAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GGG‑TG‑AAC‑TTTAAGGAG‑AAG‑T‑C‑GGGG‑TTAACCCCCA‑ACAA‑TTTTTCG‑‑‑TA‑CTTTTAAAAC‑CGGGATATG‑AAATccccggcc                                                                                                                                                                      >  1:183985/1‑234 (MQ=255)                                                                           tAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGGGAAACGCCGGAACA‑G‑G‑CGG‑TGGGG‑GGG‑GG‑AAT‑TTATGAAAG‑AAT‑A‑C‑GGAG‑TTACCCCCCA‑CC‑A‑TTTTTTG‑C‑TA‑TTTAAAAAAA‑TGAAaaata‑aataacgggaa                                                                                                                                                                       >  2:220808/1‑231 (MQ=255)                                                                            aaaaCAATGAAAAATCTGCAAAATTGGGTAGGTATTATTCGCAACGAGAAAAATGAAATCTGTATAACGCGTCTCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATGGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  2:695165/236‑1 (MQ=255)                                                                            aaaaCAATGAAAAAGCTTCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  1:1184427/236‑1 (MQ=255)                                                                            aaGACAATGAAAAAGCTGCAAATTGCTGTAGGTTTTATTCTCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAAGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  1:836095/239‑1 (MQ=255)                                                                            aaGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  1:1097480/239‑1 (MQ=255)                                                                            aaGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  1:959596/239‑1 (MQ=255)                                                                            aaGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTAGTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  1:1311021/239‑1 (MQ=255)                                                                            aaGACAATGAAAAAGCGTCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  1:776352/239‑1 (MQ=255)                                                                            aaGAAAATGAAAAAGCTGGAAATTGCGGTATGTATTAGTCGAAAAGAGAACAATGAAATCGTTATAACGCGTGGCGCAGCAGATGCGCACATGGCGAAGAAACTGGAGTTGCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TTGAATATG‑AATTCCCGGACAg                                                                                                                                                                     <  2:744241/239‑1 (MQ=255)                                                                                cAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGAGCCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGc                                                                                                                                                                   <  1:1304378/237‑1 (MQ=255)                                                                                 aaTGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCata                                                                                                                                                                <  1:1146650/239‑1 (MQ=255)                                                                                   tGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAACATTGAAATGGGTGGAACACCGGAACA‑G‑G‑CGG‑G‑GGG‑GGGCGG‑AAC‑TTCA‑GAGA‑AAG‑A‑C‑AGGAGTTACCCCCCA‑AC‑C‑ATTTTCT‑C‑CT‑TTTTAAAAAA‑GGGAAAAAG‑AATTCCCCGACACGCaaa                                                                                                                                                                >  2:309726/1‑236 (MQ=255)                                                                                   tGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAAAGGGGGAAACGCCGGAACA‑G‑G‑CGGCG‑GGG‑GGG‑GG‑AAC‑TCCGCGAGA‑CAA‑T‑C‑GGGG‑TTCCCCCCCA‑CC‑A‑TTTTTCT‑C‑GC‑TTTCAAAAAC‑GAGGATATA‑ATTTCCCGCAcgacacca                                                                                                                                                                >  2:31153/1‑231 (MQ=255)                                                                                   tGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGATTCCCGGCGGGAAAATTGAAATGGGGGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GTG‑GG‑AAA‑ATCAGGGAA‑AAGAT‑C‑GGGA‑TAACCCCCC‑‑CC‑A‑ATTTTCG‑C‑CA‑ATTTAAAAACATGGaaataaaatacccgaacagaagaaa                                                                                                                                                                >  2:1187866/1‑216 (MQ=255)                                                                                    gAAAAAGCTGCAAATTGCGGTAGGTTTTATTCCCAACAAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑aa                                                                                                                                                           <  2:555138/239‑1 (MQ=255)                                                                                        aaGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACt                                                                                                                                                       <  2:211840/239‑1 (MQ=255)                                                                                                     cGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCCTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGGAATGGGTGAAACGCCGGAACC‑G‑T‑CGA‑G‑GGG‑GCG‑TG‑AAC‑ATCCGTGAG‑AAA‑G‑C‑GGGG‑GTACGCACCC‑CC‑C‑TCGTTCG‑C‑TC‑TTCGATAAAC‑AGGGATAAT‑AAATACCCGCCAGACATA‑T‑Aaaacacagtgcttgtgtg                                                                                                                                          >  2:1197689/1‑225 (MQ=255)                                                                                                         aggtaTTATGCGCAACGAGAACAATGAAATCTTTTTAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAAGTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCg                                                                                                                                      <  1:99203/239‑1 (MQ=255)                                                                                                                      aaCGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑G‑GGG‑GCG‑TG‑GAC‑TTCAGGAAG‑AAA‑A‑C‑GGGG‑TTACCCCCCA‑AA‑A‑TTTTTTG‑C‑TA‑TTTTAAAAAC‑CGGGATATG‑AATTTCCCGACAGGCCTA‑T‑AAAACTGTGGGTTTGGCTGGTTG‑GAAGC‑TTGGGAgg                                                                                                                       >  1:418647/1‑239 (MQ=255)                                                                                                                       acggaaaCAATGAAATTTTTTAAACGGGTCGGGCAGCAGATGCGAACTGGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAAGGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAggg                                                                                                                      <  2:260671/234‑1 (MQ=255)                                                                                                                          gaaaCAATGAAATCTTTATAACGGCGCGGGAAGCAGATGGCCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AAGTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAg                                                                                                                   <  2:687600/237‑1 (MQ=255)                                                                                                                            agcAATGAAATCTTTAAAACGCGTCGCGGAGCAGATGCGCCCATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGcc                                                                                                                 <  1:1025780/237‑1 (MQ=255)                                                                                                                             acaaataaaTCTTTATAACGCGTCGGGCAGCAGATGCCCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCg                                                                                                                <  1:13358/233‑1 (MQ=255)                                                                                                                             aaaaTGAAATTTTTATAACCCGTCGCGAAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCg                                                                                                                <  2:273175/237‑1 (MQ=255)                                                                                                                             aaaaTGAAATCTTGATAACCCGTCGGGCAGCAGATGCGCCAATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AAGTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCg                                                                                                                <  1:603326/237‑1 (MQ=255)                                                                                                                             aCAATGAAATCTTTATAACGCGGCGGGCAGCAGATGCGCAAATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCg                                                                                                                <  2:679696/239‑1 (MQ=255)                                                                                                                              aaaTGAAATTTTTATAACGGGGGGGGCAGGAGATGCGCACAGGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGGGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCg                                                                                                                <  2:418647/237‑1 (MQ=255)                                                                                                                                  gAAATCTTTAAAACCCGTCGCGCAGCAGATGCGCAAAGGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTgggg                                                                                                           <  2:70067/239‑1 (MQ=255)                                                                                                                                     aTCTGTAAAACGCGTCGCGCAGAAGATTCGCACATGGCGAATAAGCTGGAGTTGCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑G‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTaa                                                                                                        <  1:740134/239‑1 (MQ=255)                                                                                                                                         ttATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑GG‑GAA‑TTCCAGAAA‑AAA‑G‑C‑GGGG‑TTACCCCCCACAC‑T‑TTTTTC‑‑C‑CA‑TTTTAAAAAA‑CGGAAAATA‑AAATCCCGCGCAAGCCTT‑T‑AAACCTTGGGGTTTGGGTGGGCG‑AACGCGTGGGAGGGGGGGGCGGGGGGGaaaag                                                                                                     >  2:1025780/1‑237 (MQ=255)                                                                                                                                         ttATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑G‑GGG‑GCG‑TG‑GAC‑TTCAAGGAA‑AAG‑G‑C‑GGGG‑TTAACCCCCA‑AA‑A‑TTTTTTG‑CGCT‑TTTGAAAAAC‑T‑GAAAATGAAATTCCCCCA‑AGGGAAA‑T‑AAAACTGTGGGTTTGTGGGGTGC‑AACGC‑GGGGAGggggggggggggggggaaaaa                                                                                                    >  2:1215480/1‑222 (MQ=255)                                                                                                                                                  gTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑TGGGG‑GGG‑GG‑AAC‑TCAAGGAAG‑AAG‑G‑C‑GGGA‑‑TACCCCCCC‑AC‑ATTTTTTCT‑C‑TA‑TTTGAAAACA‑TGGAAATTG‑AATTTCCGG‑CAGGCATA‑TAAACCCTTGGGTTTTGGCGGGTCG‑AACAC‑GGGGAAGGGGGACCGGGGGGAAAAAAA‑GGGCAAcc                                                                                           >  2:442103/1‑239 (MQ=255)                                                                                                                                                   ttccgcgGCAGTTGCGCACATGGGGAATAAACGTGGGTTTCCCGGCGGTAAAATTGAAATGGGTGGAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCg                                                                                         <  2:851039/236‑1 (MQ=255)                                                                                                                                                           aagATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCGCCA‑CC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGAAAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACg                                                                                                                                 <  1:835600/192‑1 (MQ=255)                                                                                                                                                              aTGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑G‑TGG‑GGG‑TG‑GAA‑CTCAGGGAG‑AAG‑A‑C‑CGGG‑GTAACCCCCC‑AC‑A‑TTTTTCG‑C‑TC‑TTTTGAAAAA‑TGGTAAATG‑AATTTCCCGACACGCCTATT‑AACACAG‑GGTTTTGGCTGGTTGTCAGCC‑GGGGAAGGGGGGCGTGGGGGGAAAGAG‑GGACAACCAgggaggggggg                                                                               >  2:494315/1‑230 (MQ=255)                                                                                                                                                               tGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATg                                                                              <  1:442103/238‑1 (MQ=255)                                                                                                                                                                gcgcACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑A‑GGT‑GCG‑GG‑AAC‑TTCAGGAGA‑AAG‑G‑C‑GGGG‑TTATCTCCAC‑AC‑A‑CTTTTCG‑C‑TC‑TTTGAAAAAC‑AGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACATTGGTTTTTGTGGGGAG‑GAGGC‑GAGGAGAGGGGGGCGTGGGGGAAAAAAGGGGCAACCCgggcgaggaggcg                                                                             >  2:95352/1‑228 (MQ=255)                                                                                                                                                                  gcACAGGGCGAAAAAGCGGGAGTTTCCCGGCGGTAAAATTGAAATGGGGGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTGAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCACA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCg                                                                           <  2:1146473/238‑1 (MQ=255)                                                                                                                                                                      aTGGGGAATAAACTGGAGTTTCCCGGGGGTAAAATGGAAATGGGTGAAACGCCGGAACG‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTggt                                                                      <  2:1144389/239‑1 (MQ=255)                                                                                                                                                                       tGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGGCGGAACA‑G‑G‑CGG‑T‑GGT‑TCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTggtc                                                                     <  1:62903/239‑1 (MQ=255)                                                                                                                                                                       tGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTggtc                                                                     <  1:304443/239‑1 (MQ=255)                                                                                                                                                                       tGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTggtc                                                                     <  1:1215480/239‑1 (MQ=255)                                                                                                                                                                                  cTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGG‑GGG‑GG‑AAA‑TTTAGGGAA‑AAA‑G‑A‑GGGG‑TTAACCCCCC‑AC‑A‑ATTTTTT‑C‑TATTTTGAAAAAA‑TGAATAATG‑ATTTCCCGGCCA‑GCATA‑T‑AACACCTTGGGTTTGGCGGGTCG‑CAAGC‑TGGGAGGGGGGGCCGGGGGGTAAAAAG‑GGGAAACCGGGGGGGGGGGTGGGGCTggtgggcgtaaagcg                                                          >  2:772462/1‑230 (MQ=255)                                                                                                                                                                                                  gTAAAATTTAAAGGGGGGAAACGCCGGAAAG‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTCCCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑GGGAATATG‑AATTCCCGGACGGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGGGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCgcc                                           <  2:978736/238‑1 (MQ=255)                                                                                                                                                                                                  gTAAAATTGAAATGGGTGAAACTCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTCTAG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACTC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGAGGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCgcc                                           <  1:872237/238‑1 (MQ=255)                                                                                                                                                                                                     aaaTTGAAATGGGGAGAACCCCGGAAAA‑G‑G‑GGG‑T‑GGT‑GGG‑TT‑AAC‑CTAAGGAAA‑GAG‑G‑C‑GGGA‑TTTCCCCCCA‑AC‑A‑TTTTTCG‑C‑TT‑TTTGAAAAAC‑TGGAATAGG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCgccagc                                        <  2:357859/238‑1 (MQ=255)                                                                                                                                                                                                                      ccGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GGG‑TG‑AAC‑TTCAGGAAG‑AAG‑G‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGG‑AGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCg                     <  1:772462/238‑1 (MQ=255)                                                                                                                                                                                                                       cGCCGGAACA‑G‑G‑CGG‑T‑TGT‑TCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTTCCCCCCA‑AC‑A‑TTTTGCG‑C‑TA‑TTTGAAAAAC‑TGGAATAGG‑AATTCCCGGACAGGCATA‑T‑AACTCTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCg                     <  1:95352/239‑1 (MQ=255)                                                                                                                                                                                                                           ggAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCCGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AAAGC‑TGGGAAGGGGAGCCGTGGGGGAAAGAA‑GGGCAACCCGGGGGGGGGGGGTGGCTGGTGGGTGTTAAAGCCGATGATTTTCCCCCAGCAAAGGAAACGGGaaagtggaaga                 >  2:716281/1‑230 (MQ=255)                                                                                                                                                                                                                                  g‑g‑CGG‑T‑TGT‑GCG‑TG‑AAC‑GTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCg                     <  1:154205/229‑1 (MQ=255)                                                                                                                                                                                                                                            ggt‑gCG‑GT‑AAC‑TACAGGAAG‑AAG‑T‑G‑GGGA‑TTCCCCCCCA‑AC‑A‑TTTGTCG‑C‑TA‑TTTGAAAAAC‑GTGAATATG‑AATCCCCGGAAAGGCATA‑T‑AACACGGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTg      <  2:954862/238‑1 (MQ=255)                                                                                                                                                                                                                                                tcG‑TT‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAgg  <  1:220808/238‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                   GTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCGCGCAGCAGATGCGCACATGGCGAATAAACTGGAGTTTCCCGGCGGTAAAATTGAAATGGGTGAAACGCCGGAACA‑G‑G‑CGG‑T‑GGT‑GCG‑TG‑AAC‑TTCAGGAAG‑AAG‑T‑C‑GGGA‑TTACCCCCCA‑AC‑A‑TTTTTCG‑C‑TA‑TTTGAAAAAC‑TGGAATATG‑AATTCCCGGACAGGCATA‑T‑AACACTGTGGTTTTGGCTGGTCG‑AACGC‑TGGGAAGGGGAGCCGTGGGGTAAAGAA‑GGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGG  >  NC_000913/110963‑111434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑