BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,135,439	T→G	12.0%	T311P (ACC→CCC)	pitB ←	phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,135,439	0	T	G	12.0%	301.6 / 2.6	126	T311P (ACC→CCC)	pitB	phosphate transporter
Reads supporting (aligned to +/- strand): ref base T (28/75); new base G (17/3); total (48/78)
Fisher's exact test for biased strand distribution p-value = 1.77e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GCCATGATGATCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATC  >  NC_000913/3135204‑3135671

cccaggagtatCCCCACCGGGGCGTACTCAATGGTGCTTAACAAATCGCTGGGAAGTTTTGTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGTTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTAtt                                                                                                                                                                                                                                        <  2:548122/230‑1 (MQ=255)

        tttCCACCCCTGGGCGGACCAAAGGGTGCTTACCATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCGTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GGTTTAACGCGCGTAATAGCATCAAAGGTATTTGCCGGAt                                                                                                                                                                                                                                <  2:856186/237‑1 (MQ=255)

        gatCCACCCCGGGGTGTTCTTAATGGTGCTTAACCTATCGCGGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGGTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGAt                                                                                                                                                                                                                                <  2:474394/239‑1 (MQ=255)

               cccGGAGCGTACTCAATGGTGCTTAACATTTCGCTGCGAAGTTTTTTCAGGCGGTTTTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTg                                                                                                                                                                                                                         <  1:700158/238‑1 (MQ=255)

                 cGGTGCGTACTCAATGGTGCTTAACATTTCGCTACGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGaa                                                                                                                                                                                                                       <  1:469186/239‑1 (MQ=255)

                   gAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GGTACATTCCATATTGCCTGGG‑CGCAT‑C‑CGTTTTACGCGCCCAAAAACATACAAGGGATTTTCCGGAGGAAAggaaaa                                                                                                                                                                                                                      >  2:68431/1‑233 (MQ=255)

                    aGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GGAACTTTCCATATTGCCTGGC‑GGCAT‑C‑CCTTTAAAGCGCGCAATAGCATCAAAAGTATTTTCCGGCTGCCCGTGaaaa                                                                                                                                                                                                                     >  1:1035969/1‑237 (MQ=255)

                    aGCGTACTCAATGCTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACCTTCCCAATTTCCTGGC‑AGCATCC‑GTTTTAATG‑ACGCAATTGCCACCAAAGTACTTGCCCGAGGCCAGTTaaaa                                                                                                                                                                                                                     >  2:883289/1‑237 (MQ=255)

                      cGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGGAAACTTTCCA‑ATTGGCCGGG‑AGGAT‑CGGTTTAAACGCGCGCAAAACATTCAAAGGAAttttcgggtgtaagtgaaaacct                                                                                                                                                                                                                   >  1:585543/1‑219 (MQ=255)

                            cAATGGTTCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGGCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACt                                                                                                                                                                                                            <  1:1249841/239‑1 (MQ=255)

                               tGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTTGCAGTTTCGCTAGCCTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGc                                                                                                                                                                                                         <  1:99659/239‑1 (MQ=255)

                                ggtacctAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACGCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCg                                                                                                                                                                                                        <  1:1195351/233‑1 (MQ=255)

                                  ggCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGt                                                                                                                                                                                                       <  1:966831/237‑1 (MQ=255)

                                   gCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCCGGC‑AGCAT‑C‑GTTTTTACCCGCCCCAAAGGATCAAAGGGATTTTCCGGATGGCAGTGAAACCCTGTTACtttgggg                                                                                                                                                                                                      >  1:534693/1‑233 (MQ=255)

                                    cTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCCAAGGGATTTTCCGGATGACAGTGAAACTCTGTTACTTTCGttc                                                                                                                                                                                                     >  1:752449/1‑236 (MQ=255)

                                     ttAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCa                                                                                                                                                                                                   <  1:1102946/239‑1 (MQ=255)

                                       aaCATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCCTATTTCCCGGC‑AGCAT‑C‑GTTTTTAAGCCCGCAATAGCATCAAAGGGATTTGCCGGATGTAAGTGGAACCTCGTTAATTCCGTCCCATc                                                                                                                                                                                                 >  1:101199/1‑239 (MQ=255)

                                       aaCATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAAATTTCCACATTTCCCGGCGACCAT‑C‑GTTTAAGCGCACCCAAAAGACACAAAGG‑ATTTCCCCGGTGACAAGGAAAcatcttttatttcgggcctac                                                                                                                                                                                                 >  2:656882/1‑219 (MQ=255)

                                            atCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTTCCTGGC‑CGCAT‑A‑CTTTTTAAGACCGCAATACCCTCAAAAGGATTTGCCGGTTGAACGGGAAAATCTGTTACTTTCGTGCCacacgacc                                                                                                                                                                                            >  2:854218/1‑232 (MQ=255)

                                             taagagaccggTTTTTTCAGGAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGGTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGa                                                                                                                                                                                           <  1:685675/229‑1 (MQ=255)

                                              cGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCCTATTTCCTGGC‑AGCAT‑C‑GGTTTAACCCGCGCAAAAGCATCCAAGGGATTTTCCGGATGACCGTGAAACTCTGTTACTTGCCTGCCCCCAGCCGAt                                                                                                                                                                                          >  1:813005/1‑239 (MQ=255)

                                              cGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCCTATTGCCTGGG‑AGCAT‑C‑GTTTTAAAGCGCGCACAAGCATCAAAGGGATTTGCCGGATGACAGTGAAAACCTGTTACTTTCGGGCCATCAGTccgt                                                                                                                                                                                          >  1:504197/1‑236 (MQ=255)

                                                 tGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACATCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑CTGACCTTCCCTATTTCCTGGC‑AGCCC‑A‑CCTTTTACACGCGCCAAAGCATCTATGCTAGTTGCCGGATGAAAAGGAAACACCGCTACTCCCGTACCAACACTCCAtgca                                                                                                                                                                                       >  2:558780/1‑238 (MQ=255)

                                                  gggAAGTTTTTGCAGCGGGGTCTGGTCTTCTTTACGGACGCCTGGCAGTTTCGGTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAtgc‑tg                                                                                                                                                                                     <  1:364021/237‑1 (MQ=255)

                                                                             ttttttGCTGGCCCCTGGGAGGTTCGCGCGGTTCGGGGGGGTATCAGAGATGCACTGCATAAGGGGGCCAAGCTGGCTGCGTTGAGTCTGCCTTAACGGCTC‑GTAACTTGCCATATTGCCTGGC‑AGCAT‑C‑GGTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCa                                                                                                                                                          <  2:417822/236‑1 (MQ=255)

                                                                               cttTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGTCCGGC‑CGCAT‑C‑GTTTTTAAACGCGCCACAGCATCAAAGGGATTTTCCGGATGACAGTTGAAATCTTTTACTTGCGTGCCCTCAGCCGAGGC‑TGCCGGCAAAGGGGGGTcccaccacataaa                                                                                                                                                         >  2:517902/1‑227 (MQ=255)

                                                                                       aCGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCCAAGGGATTTGCCGGATGACCGTGAAAATCTGTTACTTGCGTGCCATCAGTCGATTC‑TGCCGGCCATAGAGGTTCCTACCCAATCAACTTCTGCg                                                                                                                                                 >  1:361870/1‑238 (MQ=255)

                                                                                            tGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTTCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTCCGGCGGt                                                                                                                                            >  1:54550/1‑238 (MQ=255)

                                                                                              gCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCCTATTTCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAAAAGCATCAAAGGGATTTTCCGGATGACAGGTAAACACTTTTTATTGCGTGCCATCCAACGAGTC‑TGCCGGCAAAGGGGGGTCCATACCAATCAACCTCTTCGGCGGTTCa                                                                                                                                         >  2:1251427/1‑239 (MQ=255)

                                                                                                 gTTTCGCTTGCTTCGCGGAGGTATGCGTGATTCCCAGCATAATGCGGGGCAGCTGGCTGCGCTGACCCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGGGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGa                                                                                                                                      <  2:504197/239‑1 (MQ=255)

                                                                                                   ttccctggtttCGCGGTGTTTTCAGAGATGCACAGCTTAAGGCGGCGCGGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATg                                                                                                                                    <  2:24537/230‑1 (MQ=255)

                                                                                                   ttCGCTAGCTTCGCGGAGGTTTCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATg                                                                                                                                    <  1:656882/239‑1 (MQ=255)

                                                                                                   ttCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAAAGCGCGCAATAGCATCAAAGGTATTTTCCGGGTGACAGTGAAACTCTGTTATTTTCGTGCCATCAATCGATGC‑TGCAGGCAATAGGGGTTCCATCGCAAACAACTTTTGCGGcgagtacgggag                                                                                                                                    >  2:1325071/1‑228 (MQ=255)

                                                                                                   ttCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCAAATTTCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCCATAGCATCAAAAGGATTTTCCGGATGACCGTGAAACTCTGTTAACTTCGGGCCATTCTGCGATGC‑TGCAGGCAATGGAGGGTCCATCGCAATCaaactttccggcgggtccgggg                                                                                                                                     >  1:423271/1‑218 (MQ=255)

                                                                                                     cGCTGGCTTCGGGGAGGTATCAGAGATGCACAGAATAATGCGGCGCAGCTGGCTGCGCTGACTCACGTTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCt                                                                                                                                  <  2:54550/239‑1 (MQ=255)

                                                                                                        tAGCGTCGCGGAGGTAACAAGGATGCCCAGGATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGtt                                                                                                                               <  1:1122062/239‑1 (MQ=255)

                                                                                                         aGCTTCGGGGGGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTg                                                                                                                              <  1:93061/239‑1 (MQ=255)

                                                                                                              cgcgGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGt                                                                                                                         <  1:287419/239‑1 (MQ=255)

                                                                                                               ggtggagtaacaggagtGCACAGCATAATTGGGCGGAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTa                                                                                                                        <  1:1239886/223‑1 (MQ=255)

                                                                                                                   aGGTATCAGGAAGGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGAATCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGa                                                                                                                                      <  1:780760/221‑1 (MQ=255)

                                                                                                                    ggTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTTGC‑AGCAT‑C‑GCTTTAACCCCCGCAATAGCCTCCAAAGTATTTGCCGGGTTGCCGTGAAACTCTGTTACTTTGGTGCCATCAGTCGATGC‑CGCAGGCAATGGGGGTTTCAACGCAATCAACTTCTTCGGCGGTTCAGGATgtctttggtgggatggtt                                                                                                                   >  2:798099/1‑222 (MQ=255)

                                                                                                                     gTATCAGATATGCAACGCATAATGCGGCCCAGCTTGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCAGCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGtt                                                                                                                   <  2:981128/238‑1 (MQ=255)

                                                                                                                                 cccaGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACGTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTaa                                                                                                       <  1:944500/236‑1 (MQ=255)

                                                                                                                                      cATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTTCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCa                                                                                                                                                                                                   >  1:511355/1‑142 (MQ=255)

                                                                                                                                      cATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTTGC‑CGCAT‑C‑GTTTTAAAGCGCGCAATAGCATCCAAAGTATTTGCCGGATGACAGGGAAACTCTGTTACTTGCGTGCCACCAGTCGGTGCATGC‑GGGAAAAGAGGGTCCATCGCAATCAACATCTTCCGCAGTTTACGAGGCCGTTGCGGGTAGGGTTCGGAGTTGGTAAAggaca                                                                                                 >  2:1066973/1‑236 (MQ=255)

                                                                                                                                      cATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGGATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCCTCGCAATCAACTTCTGCGGCCGTTCAGGATGCTGTTGCAGGTAGTGTTCCCAGTTGGGAAAGGCa                                                                                                  >  1:1080076/1‑238 (MQ=255)

                                                                                                                                      cATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAC‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCa                                                                                                                                                                                                   <  2:511355/142‑1 (MQ=255)

                                                                                                                                       aTAATGGGGCCCAGGTGGCTGGTGGTACTAACGCTTAGCGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATc                                                                                                <  2:361870/239‑1 (MQ=255)

                                                                                                                                        tAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATcg                                                                                               <  1:517902/239‑1 (MQ=255)

                                                                                                                                        tAATGCGGCCCAGGTGGCTGCACTGGCTCACGCTTAGCGGCTC‑GTACCTGTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCTCGAAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACGTCTGCGGCAGTTCAGGAGGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATc                                                                                                <  2:629998/238‑1 (MQ=255)

                                                                                                                                            gtctcccAGCTTGTTGCGCTTCCTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGGAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGGCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGcggg                                                                                           <  2:375253/233‑1 (MQ=255)

                                                                                                                                             cggcgCAGCCGGCTTCGCTGACTCCCCTTTACCGGCTG‑CTAAATTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGGGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGcgggt                                                                                          <  2:1261051/239‑1 (MQ=255)

                                                                                                                                              ggcgCAGCTGGCTGCGCTTCCTTCCGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGcgggta                                                                                         <  2:1080076/239‑1 (MQ=255)

                                                                                                                                                cgcAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCCTATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAAATCTGTTACTTGCGTGCCATCCTTCGCTGC‑TGCAGGCCATGGAGGTTCCATCGCAATCAACTTCTGCGGCCGTTCAGGATGCTGTTGCAAGTGTTGTTCGTAGTTGGTAACGGCATCCcgggtacg                                                                                       >  1:285144/1‑239 (MQ=255)

                                                                                                                                                  cAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAgg                                                                                                                          <  1:597624/202‑1 (MQ=255)

                                                                                                                                                   gggttgggtcggttacgtccGCTTAACGGCTC‑GTAACTTTCCATAGTGCCTGGC‑AGCGA‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGcgggtacgggt                                                                                    <  2:101199/220‑1 (MQ=255)

                                                                                                                                                    gctggctgCGCTGGCTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGcgggtacgggta                                                                                   <  1:1325071/239‑1 (MQ=255)

                                                                                                                                                      tggctgCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGcgggtacgggt                                                                                    <  1:215740/236‑1 (MQ=255)

                                                                                                                                                              cTGACTCACGCTTTACGGGTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGc                                                                         <  1:690726/239‑1 (MQ=255)

                                                                                                                                                                  cTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATGGCCGGa                                                                     <  1:1293425/239‑1 (MQ=255)

                                                                                                                                                                   tCACGCTTAACGGTTC‑TTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGAc                                                                    <  1:1138426/239‑1 (MQ=255)

                                                                                                                                                                              ggCTC‑GGAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTGAACGCGCGAAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACGTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATAtt                                                         <  1:883289/239‑1 (MQ=255)

                                                                                                                                                                                 tC‑GGAACCTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTTCCGGATGTAAGTGAAACTCTGTTACTTGCGGGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTTCATCGGCATCAACTTCTGCGGCAGCTCATGATGCCGTTGCAGGGTGTGGTCGCAGGTGGGGACGGCATACCCGGGACGGGGCATTTTATAGTCGGagggatgtgtaatga                                                       >  2:163221/1‑224 (MQ=255)

                                                                                                                                                                                    gTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGGGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTgacga                                                    <  1:220688/239‑1 (MQ=255)

                                                                                                                                                                                      aaCCTTCCATTTTGCCTGGC‑CGCCT‑C‑GGTTTAACGGCGGCAATAGGATCCAAGGGAATTGCCGGATGGCAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGG‑TGCAGGCAAGGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGacgacg                                                  <  1:678718/239‑1 (MQ=255)

                                                                                                                                                                                            ccATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGa                                                                     <  1:835550/214‑1 (MQ=255)

                                                                                                                                                                                              atatTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCcagc                                          <  1:991944/239‑1 (MQ=255)

                                                                                                                                                                                               atttGCCTGGC‑AGGAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCcagca                                         <  1:383010/237‑1 (MQ=255)

                                                                                                                                                                                                 tttccgtgc‑agcaT‑C‑GTTTTAACCCGCGCAATTGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCcagc                                          <  1:404848/229‑1 (MQ=255)

                                                                                                                                                                                                   gCCTGGG‑CGCAT‑G‑GGTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGGTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAgggg                                     <  2:1090623/239‑1 (MQ=255)

                                                                                                                                                                                                   gCCTGGC‑AGTAT‑C‑GTTTTAACGCGCGAAATAGCCACAAAGGGATTGGCCGGGTGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAgggg                                     <  2:50911/239‑1 (MQ=255)

                                                                                                                                                                                                   gCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGCTGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCCGGTAGGGTTCGAAGTTGGCAACGGCATTCCGGGTACGGGTACTTTCATAGCCGGACGCATTATTATTGAGCACGAACCCCGCAgggg                                     >  1:611019/1‑239 (MQ=255)

                                                                                                                                                                                                     cgggC‑AGCAA‑C‑GTTTTAACGCGGGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCa                                   <  1:798099/237‑1 (MQ=255)

                                                                                                                                                                                                        gc‑agcaT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGt                                                                                                                         <  1:1152758/150‑1 (MQ=255)

                                                                                                                                                                                                         c‑agcaT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATc                                >  1:65154/1‑238 (MQ=255)

                                                                                                                                                                                                             caT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAAc                                                                                                                                                                                                                     >  2:23399/1‑55 (MQ=255)

                                                                                                                                                                                                             caT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAAc                                                                                                                                                                                                                     <  1:23399/55‑1 (MQ=255)

                                                                                                                                                                                                               t‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGCGTTCGAAGTTGGTAACGGCATCGCGGGGACGGGTAATTTCATAGCCGGACGCCTTCATATTTCCGACGAAGCCAGCAAGGGCAATCCccacc                          >  1:343141/1‑239 (MQ=255)

                                                                                                                                                                                                                ctgtttttAACGCCCGCAATAGCATCCAAGGTTGTGGCCGGTGTGCAGGGAAACTCTGTTCCTGGCGGCCCTTCCGTCGGTGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCccac                           <  2:269785/235‑1 (MQ=255)

                                                                                                                                                                                                                actgTTTAAACCCGCGAAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGGGCCAGCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTGCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCccac                           <  2:285144/236‑1 (MQ=255)

                                                                                                                                                                                                                    ttttAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACATGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGt                       <  1:473656/239‑1 (MQ=255)

                                                                                                                                                                                                                     tttAACGCGCGCAATTGCATCAAAGGTTTTTGGCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTa                      <  2:534693/239‑1 (MQ=255)

                                                                                                                                                                                                                     tttAACGCGAGCAATAGCCTCAAAGGTATTTGCCGGATGACAGTGAAATGTTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTa                      <  1:311124/239‑1 (MQ=255)

                                                                                                                                                                                                                      ttaaccgcggcaataggtgaaaaGGTATTGCCCGGATGACAGGGAAACTCTGTTACTTCCGTGCCATCAGGCGAGGC‑TTCAGGTAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTAc                     <  2:982603/219‑1 (MQ=255)

                                                                                                                                                                                                                         ccccgcgcAATAGCATCAAAGGTATTTGCCGGAGGACAGTGAAACTTTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGGAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAg                  <  2:1144226/236‑1 (MQ=255)

                                                                                                                                                                                                                         agggcagaaatcggctcgaaGGTTTTTGGCGGATTGCAGGTAAACCGTGTTACTTGGTTGCCATCGGTCGAGTC‑TGCAGGGAATGGAGGTTACATTGCAATCAATGTCTGCGGCAGTTCGGGTTGCTGTTGGAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGGAGTCATATTGACGACGAAGACAGCAGGGGCAATCCCCACCAGTTCCAg                  <  2:465170/221‑1 (MQ=255)

                                                                                                                                                                                                                         accccgcaaatggcgtaaaaTGTTTTTGCCGGAGAGCAATGAAACTGTGTTCCGTGCGTGCCATCAGTCGAGGC‑TGCAGGCAATGGAGGTTCCATGTCAATCAACTTCTGCGGCAGTTCAGTATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCCGGTACGGGTAATTTCATTGCCTGACGTATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCAGCAGGACCAg                  <  2:641761/222‑1 (MQ=255)

                                                                                                                                                                                                                         aCGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCGGCAGGGGCAATCCCCACCAGTACCa                   >  1:1144226/1‑238 (MQ=255)

                                                                                                                                                                                                                              cgcAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCCTACTGACGACGAAGCCAGCAGGGGAAATCCCCACCAGTACCACCAtt              >  1:202767/1‑238 (MQ=255)

                                                                                                                                                                                                                              cgcAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGGGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCCCCAGTCCCCGCCtt              >  1:52309/1‑238 (MQ=255)

                                                                                                                                                                                                                                        tCAAAGGTTTTTTCTGGATGACATTGAAACTCTGTTATCTGCGTGCCATCAGGGGATGC‑TGAGGGAAAATGAGGTTTCATCGGAATCAACGTACGCGGCAGTTCAGGATGTTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGAt   <  2:370145/239‑1 (MQ=255)

                                                                                                                                                                                                                                        tCAAAGGTATTTGGCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGAt   <  1:1229772/239‑1 (MQ=255)

                                                                                                                                                                                                                                        acAAAGTTGTGTGCCGGATGAAAGTGAATCTCTTTTACTTGCGTGCCATCAGTCGATGC‑TACAGGCAATGGAGTTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGAt   <  1:558780/238‑1 (MQ=255)

                                                                                                                                                                                                                                         cAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTTACGACGAAGCCAGACGGGGAGATCCCCACCAGGACCAGCATTCCCAggacact   >  1:693344/1‑233 (MQ=255)

                                                                                                                                                                                                                                           aaGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCCATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGCCGCATTCATATTGCCGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCCGCATTACCAGGCCGATc  >  1:502113/1‑237 (MQ=255)

                                                                                                                                                                                                                                           aaGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGa                                                                     <  2:457005/170‑1 (MQ=255)

                                                                                                                                                                                                                                           aaGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATc  >  1:765069/1‑237 (MQ=255)

                                                                                                                                                                                                                                           aaGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGAAATTTCATAGCCGGACGCATTCATATTGACGAAGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCCGGCCGATc  >  1:888180/1‑237 (MQ=255)

                                                                                                                                                                                                                                           aaGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGGACGGGTAATTTCATAGCCGGACGCATTCATATTTACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCCGCATTACCAGGCCGATc  >  1:838691/1‑237 (MQ=255)

                                                                                                                                                                                                                                           aaGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGGGTTCGAAGTTGGTAACGGCACCGCGGGTACGGGTAATTACATAGCCGGACGCATTAATATTGACGCCGAAGACAGCAAGGTCAAACCCCCCCCGAACCGGCATTAcacgccagacc  >  2:965445/1‑227 (MQ=255)

GCCATGATGATCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTC‑GTAACTTTCCATATTGCCTGGC‑AGCAT‑C‑GTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGC‑TGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATC  >  NC_000913/3135204‑3135671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`