BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,718	T→A	8.9%	Y179F (TAC→TTC)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,718	0	T	A	8.9%	514.1 / 3.9	160	Y179F (TAC→TTC)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (84/57); new base A (2/15); total (87/73)
Fisher's exact test for biased strand distribution p-value = 1.82e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

TGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTA  >  NC_000913/4241481‑4241955

tgtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTACGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTa                                                                                                                                                                                                                                                   >  1:1289757/1‑239 (MQ=255)

tgtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTa                                                                                                                                                                                                                                                   >  1:715964/1‑239 (MQ=255)

tgtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTa                                                                                                                                                                                                                                                   >  1:1128478/1‑239 (MQ=255)

  tttAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCGGCCGTCAGTATTCAGCCAGCTGGCATCCCCGGAACGGGCAGTAAAATCA‑TTTACGC‑AGTaa                                                                                                                                                                                                                                                  >  1:1104775/1‑238 (MQ=255)

          ccTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATAAATTCCGAGGCAACCATATAACGCCATCCGTCGGTATTCAGCCAGCTGGCATACCCGGAACGGGCAATAAAAAAA‑TTTAAGC‑AGAAAAATaaaaat                                                                                                                                                                                                                                         >  2:1156323/1‑237 (MQ=255)

                   ccTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGaaa                                                                                                                                                                                                                                >  1:1242834/1‑239 (MQ=255)

                   ccTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGaaa                                                                                                                                                                                                                                >  1:909896/1‑239 (MQ=255)

                   ccTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGaaa                                                                                                                                                                                                                                >  2:1275422/1‑239 (MQ=255)

                   ccTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCCaaa                                                                                                                                                                                                                                >  1:862156/1‑239 (MQ=255)

                        gTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGCCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAaataa                                                                                                                                                                                                                            >  1:1176355/1‑238 (MQ=255)

                              aGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGGGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCaaa                                                                                                                                                                                                                    <  1:553271/239‑1 (MQ=255)

                              aGCATGGTGCCCATAATGTG‑TCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCaaa                                                                                                                                                                                                                    <  1:507966/239‑1 (MQ=255)

                                 gtgtTGCCCAAAATTTTGGCGC‑GGAACCCTTCCGCCTTTTCTTCGTTGCCGGGCGACATCAGCCAGCGGGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCaaa                                                                                                                                                                                                                    <  2:763233/236‑1 (MQ=255)

                                   ttttcccATATTTTT‑GCCGGGGATACCGTCCGCCTTTTCCTGCTTGCCGCGGGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGt                                                                                                                                                                                                               <  2:1325451/235‑1 (MQ=255)

                                                        ggttACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCcg                                                                                                                                                                                         <  2:566811/236‑1 (MQ=255)

                                                         ggtcccttgccccgtttttgtgctgcCGCGGCACAACAGCCCGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCcg                                                                                                                                                                                         <  2:1222691/215‑1 (MQ=255)

                                                         gATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCCTACCCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAACCTAAAAGTTGCCCGAAAAAAATC‑TCAAACCGGT‑T‑AAAAAAAACCAATTTacccg                                                                                                                                                                                        >  2:1077746/1‑237 (MQ=255)

                                                             ccTTCCCCCTTTTCTTGTTTCCCGCGCGAACACCGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑G‑AAAAGAGACCAGTTTCCCGCGA‑at                                                                                                                                                                                   <  2:1111920/239‑1 (MQ=255)

                                                                   gCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGCACAGCAGTGCAGGGATACATTCCGAGGCCAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGAAAACTAAAAATTGCCCGAAAAAAATC‑CAACACGGGG‑T‑AAAAGAAACCCGTTTCCCGGCA‑ataata                                                                                                                                                                               >  2:1202582/1‑234 (MQ=255)

                                                                       gTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTgg                                                                                                                                                                          >  1:314581/1‑238 (MQ=255)

                                                                         ttttgttggcCGGGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAACATTAAGAACAGCAGTGCAGGGATACAGTCCGAGGCAAACATATAACCCCAGCCGTTAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGc                                                                                                                                                                       <  2:406673/231‑1 (MQ=255)

                                                                              gttgcCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCCGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAAGAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGtt                                                                                                                                                                  <  1:99279/238‑1 (MQ=255)

                                                                              cttgcCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACCTTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGTCCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGtt                                                                                                                                                                  <  1:424931/239‑1 (MQ=255)

                                                                               ttgcCGGGCGAAATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGtt                                                                                                                                                                  <  1:240647/238‑1 (MQ=255)

                                                                                  ccGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑AAATGAGCTGGAGCCAGTTCCGc                                                                                                                                                              >  2:68328/1‑239 (MQ=255)

                                                                                  ccGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGCATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCATCAGTATTCAG‑CAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGAGGCCAGTTTCGCa                                                                                                                                                             >  1:935550/1‑239 (MQ=255)

                                                                                   cgcgcgACATCAGCCAGCGGGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCa                                                                                                                                                             <  2:65565/239‑1 (MQ=255)

                                                                                     cccgccATCAGCCCGGGAGGACTTTCTGGCCCGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAat                                                                                                                                                           <  2:1255398/234‑1 (MQ=255)

                                                                                           aTCAGCCAGCGAGGACTTTCTGGCCAGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGATGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACa                                                                                                                                                      <  1:275042/238‑1 (MQ=255)

                                                                                            tCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑tt                                                                                                                                                   <  1:1028252/239‑1 (MQ=255)

                                                                                               gccagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACAGATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGc                                                                                                                                                <  2:314581/239‑1 (MQ=255)

                                                                                                 cgacGAAGACTTTCTGGCACGGTATCCAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACAC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGc                                                                                                                                                <  1:889025/234‑1 (MQ=255)

                                                                                                 cagcGGGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCga                                                                                                                                              <  2:864324/239‑1 (MQ=255)

                                                                                                 cagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGGGCAGGGATTCATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCg                                                                                                                                               <  1:997441/238‑1 (MQ=255)

                                                                                                    cGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGagag                                                                                                                                           >  1:1095816/1‑239 (MQ=255)

                                                                                                        ggtaaaaagaaaagggataAAGCCGGATTAAGAACAGGAGGGCAGGGATACATTCCGGGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCAtt                                                                                                                                       <  2:853619/223‑1 (MQ=255)

                                                                                                         aCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCAtt                                                                                                                                       <  2:1289757/238‑1 (MQ=255)

                                                                                                                 gCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTaa                                                                                                                               >  1:1309926/1‑238 (MQ=255)

                                                                                                                   aCGGTATACAGAAGCATTAAGAACAGCAGTGCAGGGATGCATTCCGAGGCAAACATATAACGCCAGCCGGCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAAcc                                                                                                                            <  1:374116/239‑1 (MQ=255)

                                                                                                                    cGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAAcc                                                                                                                            <  1:1275422/238‑1 (MQ=255)

                                                                                                                               agcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAACTAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGCGGCTAAACCAACGCCCAtcc                                                                                                                 >  1:349861/1‑236 (MQ=255)

                                                                                                                                gcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTTAGGCTAAACCAACGCCAATAcc                                                                                                                >  1:521570/1‑238 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGTAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAAACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGGGCATTTAGGCTAAAACAACCCCAAAAcccc                                                                                                              >  2:282411/1‑237 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACa                                                                                                                                                      >  1:845154/1‑200 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACa                                                                                                                                                      <  2:845154/200‑1 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCAtt                                                                                                                                       >  1:1244524/1‑214 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCg                                                                                                               >  1:247034/1‑238 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCg                                                                                                               >  1:343395/1‑238 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGc                                                                                                              >  1:66360/1‑239 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTCAGGCTAAACCAACGCCAATACCg                                                                                                               >  1:455818/1‑238 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGACCTGGAGCCAGTTCCGCACTATACA‑TTGGCGAGGGAATTGAGGCCAAACCAACGCCAAAACCGc                                                                                                              >  2:360493/1‑239 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTACCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATAAA‑TGGGCGAGAGCATTGAGGCTAAAAAAACGcacataaac                                                                                                               >  2:605583/1‑230 (MQ=255)

                                                                                                                                 caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAAGC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGtt                                                                                                                                                                  <  1:1219663/188‑1 (MQ=255)

                                                                                                                                        aaCAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAat                                                                                                                                                           <  2:346753/188‑1 (MQ=255)

                                                                                                                                        aaCAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGCGCATTGAGGCTAAACCAACGCCAATACCGCCaataat                                                                                                       >  1:929200/1‑239 (MQ=255)

                                                                                                                                         aCAGCAGTGCAGGGATACATTCCGAGGAAAACATATAACGCCAGCCGTCAGTATCCAGCCAGCTGCCATCACCGGAACGGCAAATAAAATAG‑TTTACGA‑AGTAAACTAAAAGTTGCCGGAAAAAAATC‑GAAAACTGGT‑T‑AAAAGAGACCATTTCCCCGCAA‑ATATGAGCTGGAGCCAGTCCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATg                                                                                                      <  1:590167/239‑1 (MQ=255)

                                                                                                                                             cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGCAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCTCGA‑ATATGAGCTGAAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCCTTGAGGCTCAACCAAACCCAAAACCCCCAAAAATggcaa                                                                                                  >  2:239683/1‑235 (MQ=255)

                                                                                                                                                tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaa                                                                                               >  1:141698/1‑239 (MQ=255)

                                                                                                                                                       aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACGCCCCATAATGCAAAAAATAACaaa                                                                                        >  1:584727/1‑239 (MQ=255)

                                                                                                                                                          cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTcc                                                                                                                                                                <  2:916923/165‑1 (MQ=255)

                                                                                                                                                          cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAAAAAAtt                                                                                      >  1:1358955/1‑238 (MQ=255)

                                                                                                                                                           aTTCGGAGGCAAACATATACCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGAAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCGGAAAATAATC‑GCAACCTGGT‑T‑AAAAGAGACCAGTTCCCGGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                    <  1:207808/239‑1 (MQ=255)

                                                                                                                                                            gtaaaaagaggcattaaaaCGCCAGCCGTCAGTATTACGCAAGCTGGCATCACGGGAACGGCCAATAAAATAG‑TTTACGC‑AATAAACAAAAAGTTGCCCAAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCATTTCCCCGCAA‑ATATGAGCTGGAGCCAGTTCCGAAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                    <  1:838305/221‑1 (MQ=255)

                                                                                                                                                               cggggaaaaCATATAGCCCCAGCCATCAGTATCCAGCAAGCTAGCACCCACGGAAGGGGAAATAAAATAG‑TTTACGC‑AGAAAACTAAAAGTTGCCCAAAAATAATC‑GAAACCTGGT‑T‑AAAAGAGACCAGTTTCCCGAGA‑ATAGGAGCTGGAGCCAGTTCCGCAATATACA‑TGGGCGAGAGCATTGAGGCAAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGa                                                                                <  1:360493/233‑1 (MQ=255)

                                                                                                                                                               cGAGACAAAAATAGAACGCCAGCCGTAATTATCCACCCAGCTGGCAGCCCCGGAACGGGAAATAAAAAAG‑TTTACGA‑AGAAAACAAAAATTGGCCAAAAAATAACC‑GAAAACTGGT‑T‑AAAAGCACCCAGTTCCCCGGGA‑AGATGAGCTGGAGCCAGTCCCGCAATATACA‑TTGGCGAGAGCATGGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGa                                                                                <  2:1309926/239‑1 (MQ=255)

                                                                                                                                                                       aCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACAAACGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACCAAACCt                                                                        >  1:1169291/1‑239 (MQ=255)

                                                                                                                                                                         atatAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCCCCGGAACGGGAAATAAAATAT‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAACCTGTT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGACCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                    <  1:889481/225‑1 (MQ=255)

                                                                                                                                                                             aaccccgccctacattttccagccaggTGGATACACAGGAACGGACAAAAAAATAG‑TTTACGA‑AGTAAACTAAAAGTTGCCAAAAAAAAACC‑GCAAACTGGT‑A‑AAAAGAGCCCAGTTTCCCGGAA‑ATAGGAGCTGGAGCCAGTTCCACAAAATACA‑TTGGCGAGAGAATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                  <  2:605023/220‑1 (MQ=255)

                                                                                                                                                                             aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaataa                                                                                            >  1:616844/1‑213 (MQ=255)

                                                                                                                                                                                  ctgCCGTCAGTTTTCAGCCAGCGGGCATCCCCGGAACGGGAAATAAAATAG‑TTTACGA‑AGGAAACTAAAAGTTGCCGAAAAATAATC‑GAAAACTGGT‑T‑AAAAGAGACCAGTTCCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGAAATATACA‑TTGGCGAGAGCATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                             <  1:663251/237‑1 (MQ=255)

                                                                                                                                                                                   aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACCGCCAATAATGCGATAAAAAAAAAATTCCGGGACAAAAccctatctctaaaac                                                            >  2:889481/1‑226 (MQ=255)

                                                                                                                                                                                     ccGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCCATAATGCGATAAATAAAAAATTCCgggcaaaaaacctctcccttataaac                                                          >  2:838305/1‑216 (MQ=255)

                                                                                                                                                                                       tgtttttatCAGCCAGGGAGCAACGCCGAAACGGAAAATAAAAATG‑TTTAGCC‑AAAAAACAAAAAGTTG‑CCAAAAAAAATT‑GCAAACGGGTGT‑AAAGGAAACAAGTTTCCCGGGA‑ATAAGAACGGGGACCAGTCACGAAAATTACA‑TGGGCGAAAAAATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGca                                                        <  2:1176355/231‑1 (MQ=255)

                                                                                                                                                                                          attttccagcaagcggaaaTCCCCGGAACGGGAAAAAAAATAG‑TTGACGA‑AGAAAAATAAAAGGTGCCCAAAAAGAATC‑GAAAACTGGT‑A‑AAAAGAGACCATTTCCCCGCGA‑AGAGGAGCGGGAGCCAGTCCCGAAATATAAA‑TGGGCGAAAGAATGGAGGCTAAACAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAg                                                     <  2:751370/221‑1 (MQ=255)

                                                                                                                                                                                          aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAACGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGACCCAGTTCCGCAATATACA‑TTGGCGAGAGAATTGAGGCTAAACCAACGCCCATACCGCCAATAATCCGATAAATAACAAATACCGGGACATAACCTACCAGATAAACAGGcccct                                                     >  1:742661/1‑235 (MQ=255)

                                                                                                                                                                                          aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGCCATAACCTGCCAGAaaaaacggcccat                                                     >  2:945222/1‑230 (MQ=255)

                                                                                                                                                                                          aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAg                                                     >  1:751370/1‑239 (MQ=255)

                                                                                                                                                                                            tATTCAGCCAGCTGGCATCACCGGAACGGGAAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAACC‑GAAACCTGGT‑T‑AAAAGAGCCCAGTTCCCCGCAA‑ATATGAGCTGGAGCCAGTCCCGCAATATACA‑TGGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAgtg                                                   <  1:68328/239‑1 (MQ=255)

                                                                                                                                                                                                 gacacaggtgcacaaCCGGAACGGGAAATAAATTAT‑TTGACGC‑AGAAAACTAAAAGTAGCCAGAAAATACTC‑GAAAACTGGT‑T‑AAAAGAGGCCAGTTCCCCGAGA‑ATTTGAGCTGGAGCAAGTCCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                              <  1:1346150/225‑1 (MQ=255)

                                                                                                                                                                                                 caccatcTGGCACCCCCAGAAGGGACAAAAAAATAA‑TCTACGA‑AGAAAAATAAAAGTAGCTCAAAATAAATC‑GAAAAATGTT‑A‑AAAAGGGACCATTTTCCCACAG‑ATATGAGCGGGACCGAGTTCCGCAATATATA‑TTGGCGAGATTATATGGGTTAAACAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                              <  2:1086463/237‑1 (MQ=255)

                                                                                                                                                                                                 agccagcGGGAATCCCGGGAACGGACAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTGGCCCGAAAATAATC‑GAAAACTGGT‑T‑AAAAGGGACCAGTTCCCCGCGA‑ATATGAGGTGGAGCCATTTCCGAAATATACA‑TTGGCGAGAGCATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                              <  1:605583/239‑1 (MQ=255)

                                                                                                                                                                                                  ccccgggtggaatctcgggaaggggAATAAAAATG‑TTTTAGCAAGAAAACAAAAAGTGGCCGAAAAATAATA‑GCAAGCTGTT‑T‑AAAAGAGACCATTGTCCGGAGA‑TTAGGGGCTGGAGCCAGTCCCGAAATACACA‑TGGGCGAGAGCATAGAGGATAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                              <  2:66360/218‑1 (MQ=255)

                                                                                                                                                                                                  acccggtgggaacccccgAAAGGGGAAAAAAAAAT‑TTTTCGCAAGAAAATAAAAATTTGCCAGAAAATAACC‑GCAACGTGGT‑A‑AAAAGAGCCCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATAAA‑TGGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                              <  2:521570/222‑1 (MQ=255)

                                                                                                                                                                                                   ccagcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAACTAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGCCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCCATAAATAACAAATTCCGGGCCATAACCTGCCAGCACAACCGGCACAACGTTTTCCg                                            >  1:1141565/1‑239 (MQ=255)

                                                                                                                                                                                                    catggggtcaccccGGAAGGGAAAAAAAAAGAG‑TTTACCG‑AAAAAACAAAAGGTTCCCAGAAAAAAATC‑GCAAACTGGT‑A‑AAAAGAGCCCAGTTCCCCGCGA‑ATGTGAGCGGGAGCAAGTCCCGCAATATACA‑TGGGGGAGAGAATTGAGGCAAAACAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                              <  2:141698/224‑1 (MQ=255)

                                                                                                                                                                                                      gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCATTATCCCCAATATTGCGATAAATAACAAATTCCGGCCCATAACCTTCCAGAAAAAAAGGAACAGTGTTGCCCGGGt                                         >  2:968645/1‑239 (MQ=255)

                                                                                                                                                                                                      gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAgtg                                                   >  1:728733/1‑229 (MQ=255)

                                                                                                                                                                                                      gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTCCCAGATAAACAGGCACAAGGTTGTCCGGGt                                         >  2:981172/1‑239 (MQ=255)

                                                                                                                                                                                                           aagtcccGGAACGGGGAAAAAAAAAGTTTTACGA‑GGAAAGAAAAAAGTTGCACGAAAATAAGC‑G‑AAACCGGT‑TAAAAAGAGCCCGTTTCCCCGAGA‑ATAGGAGCTGGAGCAAGTCCCGAAATATACA‑TTGGCGAGAGCATTGAGGCTAACCAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTtat                                     <  2:206934/234‑1 (MQ=255)

                                                                                                                                                                                                            aTCCCCGGAACGGGCAA‑AAAATAGTTTTCCGA‑ATAAAATTAAAAGTTCCCGAAAAAAAATC‑G‑AAACCGGT‑TAAAAAGAGACAGGTTCCCCGGGA‑ATTGGAGCTGGGGCCAGTTCCGAAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTtata                                    <  1:628645/238‑1 (MQ=255)

                                                                                                                                                                                                                ccGGAACGGGCAATAAAATGG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGGGACCAGTTTGCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATATCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGt                               <  1:968645/239‑1 (MQ=255)

                                                                                                                                                                                                                ccGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAACTAACAAATTCCGGGACATAACCCGCCCGCTAAACAAGCAAAGTGTTGTCCGGGTTTTTAAAgga                               >  2:1287603/1‑238 (MQ=255)

                                                                                                                                                                                                                 cggaacggaaAAAAAAATAG‑TTAACGA‑ATAAAACTAAAATGTGCCC‑AAAATAAACAGAAAACTGTT‑A‑AAAAGAGCCGAGTTTCCCGGAA‑ATAGGGGCGGGCGCCGGTTCCGCAA‑AAACATTTGGGGAAAGAATTGAGGCTAAACCACCGACAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTa                              <  2:247034/239‑1 (MQ=255)

                                                                                                                                                                                                                        ggCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTaaaa                           >  1:206934/1‑235 (MQ=255)

                                                                                                                                                                                                                        gcaaaaaaaaTTG‑TTTACGA‑ATAAAACTAAAATTTCCCAAAAAATAAGT‑GCAACCTGGT‑T‑AAAAGAAACAGGTCTCCCGCGA‑ATTGGGGCTGGCGCCACTTCCGCAATAAACA‑TTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAAcc                         <  2:1236911/231‑1 (MQ=255)

                                                                                                                                                                                                                         gCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCCGATAAAAAGGCACAGTGTTGTCCGGGTTTAAAAAGGTaaaaaaaag                      >  2:669284/1‑234 (MQ=255)

                                                                                                                                                                                                                                       ttcccc‑AGTAAAATATTAGTCGCCCGAAAAAACTC‑GGAAAGGGGA‑T‑AAAATGGCCCAG‑TTCCCGCGAGATCAGAGTGGTGGCCAGTGCCGCAATCACCA‑TTGGGAACAGCATTGAGGATAACACAACCAGAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGGCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa         <  2:584727/234‑1 (MQ=255)

                                                                                                                                                                                                                                        tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATaa                                                                           >  1:399578/1‑172 (MQ=255)

                                                                                                                                                                                                                                        tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTTTCCGGGTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCaa        >  2:583842/1‑238 (MQ=255)

                                                                                                                                                                                                                                                tAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTa  >  1:824892/1‑238 (MQ=255)

TGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTT‑GCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAACTGGT‑T‑AAAAGAGACCAGTTTCCCGCGA‑ATATGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTA  >  NC_000913/4241481‑4241955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`