BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,837	G→C	8.8%	G139G (GGC→GGG)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,837	0	G	C	8.8%	343.8 / 4.9	135	G139G (GGC→GGG)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base G (82/38); new base C (4/10); total (86/49)
Fisher's exact test for biased strand distribution p-value = 6.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

AGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACG  >  NC_000913/4241607‑4242074

agcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAACTAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGCGGCTAAACCAACGCCCAtcc                                                                                                                                                                                                                                         >  1:349861/1‑236 (MQ=255)

 gcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTTAGGCTAAACCAACGCCAATAcc                                                                                                                                                                                                                                        >  1:521570/1‑238 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGTAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAAACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGGGCATTTAGGCTAAAACAACCCCAAAAcccc                                                                                                                                                                                                                                      >  2:282411/1‑237 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCg                                                                                                                                                                                                                                       >  1:247034/1‑238 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCg                                                                                                                                                                                                                                       >  1:343395/1‑238 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGc                                                                                                                                                                                                                                      >  1:66360/1‑239 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATAAACA‑TTGGCGAGAGAATTAAGGCTAAACCAACGCCAAAACCGc                                                                                                                                                                                                                                      >  2:628645/1‑239 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCAAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCACCGCCAATACCGc                                                                                                                                                                                                                                      >  1:605023/1‑239 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTACCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATAAA‑TGGGCGAGAGCATTGAGGCTAAAAAAACGcacataaac                                                                                                                                                                                                                                       >  2:605583/1‑230 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑CCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGGGCCAGTTCCGACATATACA‑TTGGCGAGGGCATTTAGGCTAAACCAACGCCaaaaacc                                                                                                                                                                                                                                       >  2:578394/1‑233 (MQ=255)

  caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑CCAAAC‑TGCT‑T‑AAAAGAGACCA‑GTTTCCCTCGA‑ATA‑TGAGCTGCAGCCAGTTCCGCAATATACA‑TTGCCGAGAGCATTAAGCGTAAACCAACGCCCAAACCg                                                                                                                                                                                                                                       >  1:1099868/1‑238 (MQ=255)

          aCAGCAGTGCAGGGATACATTCCGAGGAAAACATATAACGCCAGCCGTCAGTATCCAGCCAGCTGCCATCACCGGAACGGCAAATAAAATAG‑TTTACGA‑AGTAAACTAAAAGTTGCCGGAAAAAAATC‑GAAAAC‑TGGT‑T‑AAAAGAGACCA‑TTTCCCCGCAA‑ATA‑TGAGCTGGAGCCAGTCCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATg                                                                                                                                                                                                                              <  1:590167/239‑1 (MQ=255)

            agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGCGAGCATTAAGGCTAAACCAACGCCAATACCGCCAATAATGCg                                                                                                                                                                                                                            >  1:704770/1‑239 (MQ=255)

            aaaacgggggggttcattcccGAGGAAAAAAAAAAACCCCAGCCGCAATTATACACCCAGCGGGCATACCCGGAAGGGAAAAAAAAATAG‑TTTACAC‑AGTAAACAAAAAGTTGCCAGAAAATAATC‑GCAAAC‑TGTT‑A‑AAAAGAGACCA‑GTTCCCCGCGA‑ATA‑TGGGCTGGAGCCAGTTCCGAAAAAAACA‑TTGGCGAGAGCATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCg                                                                                                                                                                                                                            <  2:1316403/220‑1 (MQ=255)

                 tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaa                                                                                                                                                                                                                       >  1:141698/1‑239 (MQ=255)

                           cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAAAAAAtt                                                                                                                                                                                                              >  1:1358955/1‑238 (MQ=255)

                            aTTCGGAGGCAAACATATACCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGAAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCGGAAAATAATC‑GCAACC‑TGGT‑T‑AAAAGAGACCA‑GTTCCCGGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                                                                                            <  1:207808/239‑1 (MQ=255)

                             ttCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGCGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACaactgtct                                                                                                                                                                                                           >  1:1316403/1‑233 (MQ=255)

                                cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGGGAGCATTGAGGCTAAACCAAACCCACAACCGCCAAAAATGCGATAAAAAAAAAATTCCGGGa                                                                                                                                                                                                        >  2:207808/1‑239 (MQ=255)

                                cGAGACAAAAATAGAACGCCAGCCGTAATTATCCACCCAGCTGGCAGCCCCGGAACGGGAAATAAAAAAG‑TTTACGA‑AGAAAACAAAAATTGGCCAAAAAATAACC‑GAAAAC‑TGGT‑T‑AAAAGCACCCA‑GTTCCCCGGGA‑AGA‑TGAGCTGGAGCCAGTCCCGCAATATACA‑TTGGCGAGAGCATGGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGa                                                                                                                                                                                                        <  2:1309926/239‑1 (MQ=255)

                                        aCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACAAACGCCAATACCGCCAATAATTCGATAAATAACAAATTCCGGGACCAAACCt                                                                                                                                                                                                >  1:1169291/1‑239 (MQ=255)

                                          atatAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCCCCGGAACGGGAAATAAAATAT‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAACC‑TGTT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGACCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                                                                                            <  1:889481/225‑1 (MQ=255)

                                              aaccccgccctacattttccagccaggTGGATACACAGGAACGGACAAAAAAATAG‑TTTACGA‑AGTAAACTAAAAGTTGCCAAAAAAAAACC‑GCAAAC‑TGGT‑A‑AAAAGAGCCCA‑GTTTCCCGGAA‑ATA‑GGAGCTGGAGCCAGTTCCACAAAATACA‑TTGGCGAGAGAATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                                                                                          <  2:605023/220‑1 (MQ=255)

                                              aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaataa                                                                                                                                                                                                                    >  1:616844/1‑213 (MQ=255)

                                                   ctgCCGTCAGTTTTCAGCCAGCGGGCATCCCCGGAACGGGAAATAAAATAG‑TTTACGA‑AGGAAACTAAAAGTTGCCGAAAAATAATC‑GAAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTCCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGAAATATACA‑TTGGCGAGAGCATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                                                                                                                                                     <  1:663251/237‑1 (MQ=255)

                                                    aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTGAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAAACTGCCAGATAAACa                                                                                                                                                                                    >  1:1133447/1‑239 (MQ=255)

                                                    aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACCGCCAATAATGCGATAAAAAAAAAATTCCGGGACAAAAccctatctctaaaac                                                                                                                                                                                    >  2:889481/1‑226 (MQ=255)

                                                      ccGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCCATAATGCGATAAATAAAAAATTCCgggcaaaaaacctctcccttataaac                                                                                                                                                                                  >  2:838305/1‑216 (MQ=255)

                                                        tgtttttatCAGCCAGGGAGCAACGCCGAAACGGAAAATAAAAATG‑TTTAGCC‑AAAAAACAAAAAGTTG‑CCAAAAAAAATT‑GCAAAC‑GGGTGT‑AAAGGAAACAA‑GTTTCCCGGGA‑ATA‑AGAACGGGGACCAGTCACGAAAATTACA‑TGGGCGAAAAAATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGca                                                                                                                                                                               <  2:1176355/231‑1 (MQ=255)

                                                        gTCAGTATTCACCCAGCGGGCATCACGGGAACGGGCAAAAAAATAG‑TTTACGA‑AGTAAACTAAAAGTTGCCCAAAAATAATC‑GCAAAC‑TGGT‑A‑AAAAGAGCCCA‑TTTCCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGca                                                                                                                                                                               <  1:239683/239‑1 (MQ=255)

                                                           attttccagcaagcggaaaTCCCCGGAACGGGAAAAAAAATAG‑TTGACGA‑AGAAAAATAAAAGGTGCCCAAAAAGAATC‑GAAAAC‑TGGT‑A‑AAAAGAGACCA‑TTTCCCCGCGA‑AGA‑GGAGCGGGAGCCAGTCCCGAAATATAAA‑TGGGCGAAAGAATGGAGGCTAAACAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAg                                                                                                                                                                            <  2:751370/221‑1 (MQ=255)

                                                           aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAACGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGACCCAGTTCCGCAATATACA‑TTGGCGAGAGAATTGAGGCTAAACCAACGCCCATACCGCCAATAATCCGATAAATAACAAATACCGGGACATAACCTACCAGATAAACA‑GGcccct                                                                                                                                                                            >  1:742661/1‑235 (MQ=255)

                                                           aGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAg                                                                                                                                                                            >  1:751370/1‑239 (MQ=255)

                                                                  gacacaggtgcacaaCCGGAACGGGAAATAAATTAT‑TTGACGC‑AGAAAACTAAAAGTAGCCAGAAAATACTC‑GAAAAC‑TGGT‑T‑AAAAGAGGCCA‑GTTCCCCGAGA‑ATT‑TGAGCTGGAGCAAGTCCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                                                     <  1:1346150/225‑1 (MQ=255)

                                                                  acccagcgagaaTCACCGGAACGGGCAATAAAATAG‑TTTACGA‑AGAAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑A‑AAAAGAGCCCA‑GTTCCCCGCGA‑ATA‑GGAGCGGGAGCCAGTCCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                                                     <  1:987730/228‑1 (MQ=255)

                                                                   ccccgggtggaatctcgggaaggggAATAAAAATG‑TTTTAGCAAGAAAACAAAAAGTGGCCGAAAAATAATA‑GCAAGC‑TGTT‑T‑AAAAGAGACCA‑TTGTCCGGAGA‑TTA‑GGGGCTGGAGCCAGTCCCGAAATACACA‑TGGGCGAGAGCATAGAGGATAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                                                     <  2:66360/218‑1 (MQ=255)

                                                                   acccggtgggaacccccgAAAGGGGAAAAAAAAAT‑TTTTCGCAAGAAAATAAAAATTTGCCAGAAAATAACC‑GCAACG‑TGGT‑A‑AAAAGAGCCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATAAA‑TGGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                                                     <  2:521570/222‑1 (MQ=255)

                                                                     catggggtcaccccGGAAGGGAAAAAAAAAGAG‑TTTACCG‑AAAAAACAAAAGGTTCCCAGAAAAAAATC‑GCAAAC‑TGGT‑A‑AAAAGAGCCCA‑GTTCCCCGCGA‑ATG‑TGAGCGGGAGCAAGTCCCGCAATATACA‑TGGGGGAGAGAATTGAGGCAAAACAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                                                     <  2:141698/224‑1 (MQ=255)

                                                                       gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCATTATCCCCAATATTGCGATAAATAACAAATTCCGGCCCATAACCTTCCAGAAAAAAA‑GGAACAGTGTTGCCCGGGt                                                                                                                                                                >  2:968645/1‑239 (MQ=255)

                                                                       gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAgtg                                                                                                                                                                          >  1:728733/1‑229 (MQ=255)

                                                                       gcTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTCCCAGATAAACA‑GGCACAAGGTTGTCCGGGt                                                                                                                                                                >  2:981172/1‑239 (MQ=255)

                                                                            caccccgggAAGGGGAAATAAAATAG‑TTGACGA‑AGTAAAAAAAAAGTGGCCAGAAAATAATC‑GAAAAC‑GGGT‑T‑AAAAGAGCCCA‑TTTCCCCGCGA‑ATA‑GGAGCTGGAGCCAGTTCCGCAATAGCCA‑TTGGGGAGAGCATTGAGGCAAAACAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTtat                                                                                                                                                            <  1:578394/231‑1 (MQ=255)

                                                                            aagtcccGGAACGGGGAAAAAAAAAGTTTTACGA‑GGAAAGAAAAAAGTTGCACGAAAATAAGC‑G‑AAAC‑CGGT‑TAAAAAGAGCCCG‑TTTCCCCGAGA‑ATA‑GGAGCTGGAGCAAGTCCCGAAATATACA‑TTGGCGAGAGCATTGAGGCTAACCAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTtat                                                                                                                                                            <  2:206934/234‑1 (MQ=255)

                                                                                 ccGGAACGGGCAATAAAATGG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGGGACCA‑GTTTGCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATATCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGt                                                                                                                                                      <  1:968645/239‑1 (MQ=255)

                                                                                 ccGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAACTAACAAATTCCGGGACATAACCCGCCCGCTAAACA‑AGCAAAGTGTTGTCCGGGTTTTTAAAgga                                                                                                                                                      >  2:1287603/1‑238 (MQ=255)

                                                                                  cggaacggaaAAAAAAATAG‑TTAACGA‑ATAAAACTAAAATGTGCCC‑AAAATAAACAGAAAAC‑TGTT‑A‑AAAAGAGCCGA‑GTTTCCCGGAA‑ATA‑GGGGCGGGCGCCGGTTCCGCAA‑AAACATTTGGGGAAAGAATTGAGGCTAAACCACCGACAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTa                                                                                                                                                     <  2:247034/239‑1 (MQ=255)

                                                                                         ggCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTaaaa                                                                                                                                                  >  1:206934/1‑235 (MQ=255)

                                                                                          gCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCCGATAAAAA‑GGCACAGTGTTGTCCGGGTTTAAAAAGGTaaaaaaaag                                                                                                                                             >  2:669284/1‑234 (MQ=255)

                                                                                                       tttacgcaaaaaaaagaaaaGTTCCCCAAAAATAACC‑CCAAAG‑TGGT‑A‑AAAAGAGCCCA‑GTTCCCCGGAA‑ATT‑TGGGCGGGGACCATTTCCGAAATATAAA‑TTGGCGAGAGCATGGAGGCTAACCCACCCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAgg                                                                                                                                  <  2:1133447/223‑1 (MQ=255)

                                                                                                        ttcccc‑AGTAAAATATTAGTCGCCCGAAAAAACTC‑GGAAAG‑GGGA‑T‑AAAATGGCCCA‑G‑TTCCCGCGAGATC‑AGAGTGGTGGCCAGTGCCGCAATCACCA‑TTGGGAACAGCATTGAGGATAACACAACCAGAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGGCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                                                                <  2:584727/234‑1 (MQ=255)

                                                                                                        ttACGC‑AGAAAAATAAAAGTTCCCAAAAAAAAAGC‑GAAAAC‑TGGT‑A‑AAAAGGGACCA‑TTTTCCCGCGA‑AAA‑GGGGCGAGGGCCAGTTCCGAAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATGCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                                                                <  2:1095816/239‑1 (MQ=255)

                                                                                                         ttccgacgaaaaCTAAAAGTTCCCAAAAAATTAGC‑G‑AAAA‑TGGT‑A‑GAAAAGGACCC‑GTTGCCCAGGA‑AAATTGAGTGGGGGCACGTCCCGAAATATACT‑TGGGCAAAAGAAGGAAAGCTAAACCAACCTCAATACGGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                                                                <  2:343395/230‑1 (MQ=255)

                                                                                                         tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATaa                                                                                                                                                                                                   >  1:399578/1‑172 (MQ=255)

                                                                                                         tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAAc                                                                                                                                                 >  1:130974/1‑221 (MQ=255)

                                                                                                         tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAAAGGTAAAACCAAGTTCTGGCCAGGCAg                                                                                                                               >  1:607078/1‑239 (MQ=255)

                                                                                                         tACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGGGTTTTCCGGGTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCaa                                                                                                                               >  2:583842/1‑238 (MQ=255)

                                                                                                                 tAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTa                                                                                                                         >  1:824892/1‑238 (MQ=255)

                                                                                                                 tAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGAAAAACA‑GGCACAGTGTTGTCCGGGTTTAAAGAGGAAAAACAAAGATCTGGCCAGGCAGAACCTaa                                                                                                                        >  2:682756/1‑238 (MQ=255)

                                                                                                                 tAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCCGGACACAACCTGCCAGATACACATGGCA‑AATGTTATACGGGTTTATAGAGGAAAAACCAAATTTATGCCAAGAAGAACCTaa                                                                                                                        >  2:812197/1‑238 (MQ=255)

                                                                                                                      tAAAAGTTG‑CCAAAAAAAAAC‑TCAAACTTGGT‑A‑AAAAGAGCCTA‑GTTCCCCGAGA‑ATA‑TGGCTTGGGCCCTGTCCCCAAATATACA‑TGGGGGAGAGCATGGAGGTAAAACAACCGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                                                                   <  2:824892/239‑1 (MQ=255)

                                                                                                                      aaaagGTTGCCAAAAAAAAATC‑GCAAAG‑TGTT‑T‑AAAAGAGACCA‑GTTCCCCGCAA‑ATA‑TGAGCGGGACCCATTTCCGCAATATACA‑TTGGGGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                                                                   <  1:682756/238‑1 (MQ=255)

                                                                                                                              gCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCT‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAATTAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGCGTTGTCCGGGTTTATCGAGGTAAAACCAAGTTCCGGCCAGGCCGAAACTACACTAGAAATaaaa                                                                                                           >  1:986106/1‑239 (MQ=255)

                                                                                                                               cccGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAGTAACAAATTc                                                                                                                                                                                                             <  1:55092/141‑1 (MQ=255)

                                                                                                                               cccGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGGTTATCGAGGGAAAACCAAGTTATGCCCAGGCAAAAACAAAACaaaaaaaaaaaa                                                                                                          >  2:1166159/1‑239 (MQ=255)

                                                                                                                                ccaaaaaTAATC‑GAAACC‑GGGT‑T‑AAAAGGGACCA‑GTTTCCGGGGA‑ATA‑GGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaaa                                                                                                         <  1:1034161/236‑1 (MQ=255)

                                                                                                                                   aatttgtaaaggagcaggggT‑T‑AAAAGAGACCG‑GTTCCCCGAGA‑ATA‑TGAGCGGGAGCCAGTGCCCAATTATACA‑TGGGCGAGACCATTGAGGCAAAACCAACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaaa                                                                                                         <  1:1268012/220‑1 (MQ=255)

                                                                                                                                     aataatC‑GCAAAC‑GGGT‑T‑AAAAGAAACCC‑TTTTCCCGAGA‑ATA‑TGAGCTGGACCCAGTTCCGCAATAAACA‑TTGGGGAGAGCATTGAGGCTAAACCAACGCCAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                                                                    <  1:583842/239‑1 (MQ=255)

                                                                                                                                      aataccctcAAAC‑T‑GT‑T‑AAAAGAAAACAGGTGCCCCGGAA‑ATA‑GGAGCTGGAGCCGGTCCCGAAATATCCA‑TTGGGGAGAAAATTGGGGATAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                                                                    <  1:1186289/231‑1 (MQ=255)

                                                                                                                                         atC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCAGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACAAAACCTGCCAGCTAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTCATGGCCAGGCAGAACCTAACCCAGAAATAAAAAACaagcaaac                                                                                                >  2:920094/1‑232 (MQ=255)

                                                                                                                                              cccaaatagggttaaagataaCCA‑GTTCCCCGGAA‑ATT‑TGGGCTGGAGCCAGTGCCGAAATAGACA‑TTGGGGAAGCCATTGAGGCAAACCCAACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagca                                                                                               <  1:1202582/219‑1 (MQ=255)

                                                                                                                                                      gT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTc                                                                                                                                                                     >  1:1240322/1‑159 (MQ=255)

                                                                                                                                                      gT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGATCTGGCCAGGCAGAACCTACACCAGAAAAAAAAAAAAGAACAAAAGCAAACTTaa                                                                                     >  2:34034/1‑239 (MQ=255)

                                                                                                                                                         taaaaaGAGACCG‑TTTCCCCGGAA‑ATG‑TGAGCTGGCGCCAGTTCCGAAATATAAG‑TTGGCGAGAGCATTGAGGAAAACCCAACTCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                                                     <  1:1287603/236‑1 (MQ=255)

                                                                                                                                                                  aCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGTCCAGGCAGAACCTACCCCAGCAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCacc                                                                           >  2:1325881/1‑238 (MQ=255)

                                                                                                                                                                  aCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaa                                                                                                           >  1:1210079/1‑207 (MQ=255)

                                                                                                                                                                  aCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAACCTTAAGTGAATCacg                                                                           >  1:909260/1‑239 (MQ=255)

                                                                                                                                                                  aCCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGGAAAACCAAGTTTTGGCCAGGCAGAACCTACACCCGAAATAAAAAAAAGGAAAGCAACAAATTTaaagaaaaaaac                                                                           >  2:1093831/1‑229 (MQ=255)

                                                                                                                                                                              ccggaaattggggggggACCCAGTCCCGAAATATACA‑GTGGCGGAAGCATGGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAACCTTAAGTGAATCACGACGACCGaa                                                                  <  1:34034/224‑1 (MQ=255)

                                                                                                                                                                                   ata‑gGAGGTGGAGCCAGTTCCGCAATTACCA‑TTGGCGAGAGCATTGAGGCTAAACCAACCACAACACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                                                             <  1:125352/238‑1 (MQ=255)

                                                                                                                                                                                   aattggggctggacaacgtgccacAATATACA‑TTGGCGAAAGAATGGAGGACAAACCACCCCAAACGCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                                                             <  2:349861/216‑1 (MQ=255)

                                                                                                                                                                                              gAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAAACTTAAGAGAATCACGACCACCAAAACGGTAAcagaaaaaa                                                  >  2:125352/1‑231 (MQ=255)

                                                                                                                                                                                                              ataCA‑TTGGCGAGATAATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                                                                    <  1:1122679/173‑1 (MQ=255)

                                                                                                                                                                                                               taCA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACt                                                         >  1:116596/1‑215 (MQ=255)

                                                                                                                                                                                                                  a‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCGATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgag                              >  1:432248/1‑238 (MQ=255)

                                                                                                                                                                                                                        cGAGAGCATTGAGGTTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                                                                   <  1:459284/149‑1 (MQ=255)

                                                                                                                                                                                                                        cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTAATGCAATAACCACCAATGGCACCGCCGCTCATGc                         >  1:440471/1‑239 (MQ=255)

                                                                                                                                                                                                                        cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGAGGTAACTGCAATAacaaccagaggaacccccgcagatgg                         >  2:1272187/1‑219 (MQ=255)

                                                                                                                                                                                                                        cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAAAAGGACAGCAGCAATCTTAAGGAAATAACGACGCCCGAAACGGGTACTGAAATAACCACCGAAGGCAaccccgggaaaga                         >  2:710834/1‑232 (MQ=255)

                                                                                                                                                                                                                        cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCAAAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAg                                                                                                                   >  2:459284/1‑149 (MQ=255)

                                                                                                                                                                                                                          agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCCCGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgccgcaaatgca                        >  1:45942/1‑230 (MQ=255)

                                                                                                                                                                                                                          agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAACCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCa                        >  1:248734/1‑238 (MQ=255)

                                                                                                                                                                                                                          agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAACCGGTTACTGCAAAAACCACCGAGGGACccgccagtaatgaa                        >  2:246263/1‑229 (MQ=255)

                                                                                                                                                                                                                            agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGCCAGCAGCAACCTTAAGTGAATCACGACGACCGAACCGGTTACTGCAATAACACCCCAGGGCaacccccacaatccacac                     >  2:840195/1‑222 (MQ=255)

                                                                                                                                                                                                                            agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAccc                     >  1:1209105/1‑239 (MQ=255)

                                                                                                                                                                                                                            agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATAACGACGACCGAAGCGGTACACGCAAAAACAACCAAGGGCACCGCCgatgagtaaacc                     >  2:90534/1‑232 (MQ=255)

                                                                                                                                                                                                                                        aaaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaaa                                                                                                         >  1:85622/1‑143 (MQ=255)

                                                                                                                                                                                                                                          aCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaa                                                                                                            >  1:1006240/1‑138 (MQ=255)

                                                                                                                                                                                                                                          aCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTg        >  1:727702/1‑238 (MQ=255)

                                                                                                                                                                                                                                               cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAggg                                        >  1:100027/1‑201 (MQ=255)

                                                                                                                                                                                                                                               cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGAAACCAATCAGAgcgcctgcctct  >  1:609786/1‑231 (MQ=255)

AGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAG‑TTTACGC‑AGTAAACTAAAAGTTGCCCGAAAATAATC‑GCAAAC‑TGGT‑T‑AAAAGAGACCA‑GTTTCCCGCGA‑ATA‑TGAGCTGGAGCCAGTTCCGCAATATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACA‑GGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACG  >  NC_000913/4241607‑4242074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`