Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,030,269	C→T	14.9%	S69F (TCT→TTT)	yccX →	weak acylphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,030,269	0	C	T	14.9%	86.4 / 2.1	63	S69F (TCT→TTT)	yccX	weak acylphosphatase
Reads supporting (aligned to +/- strand):  ref base C (19/33);  new base T (7/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 3.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAG  >  NC_000913/1030037‑1030490                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                   cgTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAGGAGG‑GC‑GGGCCCCGCTTTgcggt                                                                                                                                                                                                                              >  2:333084/1‑237 (MQ=255)        taaggataaggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑Gaa                                                                                                                                                                                                                      <  1:47399/239‑1 (MQ=255)            gataaggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGCGCCTGGA‑GAACcgg                                                                                                                                                                                                                  >  1:894266/1‑239 (MQ=255)               aaggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGGTTTGCGGCTGTG‑GAACggggg                                                                                                                                                                                                                >  1:1123119/1‑236 (MQ=255)                aggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGGAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGGGGGTGTG‑GAACggggggt                                                                                                                                                                                                              >  1:127419/1‑235 (MQ=255)                aggaGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGGGAAGAAGGGCAGGGGGAAAAATTAATGCAGTGGTTAAAGGGGG‑GG‑GGGCCCCGGTCTTCGGgtgtgacacgggggga                                                                                                                                                                                                               >  2:1195444/1‑227 (MQ=255)                     agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGCCGATGGCAGCGTGGAAGTGGTTGCTTGCGGGTGAGAAGGGCAGGTGGAAAAATTAATGCATGGGTTAAAGCGTG‑GC‑AGGCGGCGGTCTgccggggggaaaaaggggcgctcag                                                                                                                                                                                                          >  2:266424/1‑216 (MQ=255)                     agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAAAGTG‑GC‑GGCCCCCGGTCTCGGGGTGTT‑GAACgggggggcgggg                                                                                                                                                                                                         >  2:859797/1‑230 (MQ=255)                      gcagcaATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGcgcggggtaaacggggggtcacagg                                                                                                                                                                                                         >  2:749315/1‑218 (MQ=255)                      gcagcaATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTCACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATCCAGTGGTTAAAGAGGG‑GC‑GGACAGGGGGATGCGCGCGGG‑GAAAGGGGGCTAAGCGa                                                                                                                                                                                                        >  2:1183921/1‑239 (MQ=255)                        ataaaTGTCGAAAGTTGGCATAATTCCCGGGGTTTACGGGGGATGTCAGGGCGTAGGATTGCGCTACCGCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAAGGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAg                                                                                                                                                                                                       <  2:563506/235‑1 (MQ=255)                          caATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAACGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAGAGTG‑GC‑GGCCCCGGTGCTGGGCGCGTG‑Gaaagaggggcgcacgggcg                                                                                                                                                                                                     >  2:76248/1‑221 (MQ=255)                            aTGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGGGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGATTTGCGCGCGTA‑GAACGGGGGCGACGCGAG‑CCGc                                                                                                                                                                                                  >  1:845685/1‑238 (MQ=255)                                  aaaGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGGTGTGGGCGGGTA‑GAACAGGGGGCAAGCGAG‑ACGcgcaacc                                                                                                                                                                                            >  2:421773/1‑232 (MQ=255)                                      tCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCATGGGTTAAAGGGTG‑GCGGGCCCGCGTTTTGGGCGTGTA‑GACGGGGGGCTAAGAGAC‑GCGCATCAccccgc                                                                                                                                                                                        >  2:340488/1‑237 (MQ=255)                                        ggaaaaaatGCGTGGGTTTAGGGGGGTGTGCGGGGCGTAGGTTTCCGCAACCCCACACAGGACGAAGCGAAAAGACTGGGTTTCACCGGGTACGCCAAAAATTTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGGGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCggg                                                                                                                                                                                     <  2:845685/231‑1 (MQ=255)                                         gCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGTTCTGCCGGGGTG‑GAACGGGGGTTCCGCGGG‑CCGCGTCCTCCCCCgggg                                                                                                                                                                                    >  1:475914/1‑239 (MQ=255)                                               ttGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGGCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTa                                                                                                                                                                              <  1:1183921/239‑1 (MQ=255)                                                  ccTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTGGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTTTGCGCGTGTA‑GAACGGGGGGTCAGCGAG‑CCCACTCACTCCCCGGGGGGATTTAc                                                                                                                                                                            >  1:906926/1‑238 (MQ=255)                                                    tGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGGCCGTGTA‑GAACGGGGGCTGAGCGGG‑CCGGCGCCGCCCACGGGGGGATTAAACgg                                                                                                                                                                         >  1:338548/1‑238 (MQ=255)                                                       gTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTTTGCGCGTGTA‑GAACGGGGGCTCAGCGAG‑CCCCACCATCCCTCGGGGGGATTAACCGAttt                                                                                                                                                                      >  1:335284/1‑239 (MQ=255)                                                        tttACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAAc                                                                                                                                                                                                                     <  1:1220194/192‑1 (MQ=255)                                                             gggCGTGTTCGGGGCGAAGGATTTGGCAACCCCCACCAGGCAGAGTCAAAAAGCATGGGTTTCAACGGGTACGCCAAAAATCTTGGCGGTGGAAGCGTGGACGTGGTTGCTTGGGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                <  2:846193/239‑1 (MQ=255)                                                             gggCGAGGTAAGGGCGTAGGATTTCGCTTCACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGGGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                <  1:266424/239‑1 (MQ=255)                                                                 ggtttCGGGGCTTGGGGTTTCGCTTCCCCCCAAAGTAAGGAGCGAAAAGACTGGGTTTAACGGGGTCCGCAAAAAATGTTGCAGGTGGCAGGGTGGAAGGGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCg                                                                                                                                                             <  2:245560/235‑1 (MQ=255)                                                                                        tacaccgaccaGTACGGAGCAAAAAGGCTGGGTTTAACCGGGTACGCCAAAAATCTTGACGTTGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTgg                                                                                                                                   <  1:1135153/230‑1 (MQ=255)                                                                                        tACACCAAAAAGGACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAATTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTgg                                                                                                                                   <  2:338548/239‑1 (MQ=255)                                                                                         acacCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGc                                                                                                                                  <  1:421773/239‑1 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGGGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCa                                                                                                                           <  1:1276321/239‑1 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGGGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTTACCGGTTTTCGTATCCGTTA‑AATACATTTTAACCGT‑TTTTGC‑GGGcgc                                                                                                                           >  1:1317053/1‑237 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGCGCGCGTA‑GAACGGGTGCTCAGCGAG‑CCGACTCATCCCTCGGGGGGATTAACCGATTTTTGTATCCGTTA‑AATACATTTTACCCGT‑TTTTGC‑AGGccc                                                                                                                           >  1:512785/1‑238 (MQ=255)                                                                                               cacaGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGCGCTGCGCGGGGA‑GAAAAGGGGCTCAGCGAG‑GCCCCGCCTCCCCCGGGGGACGTTACAGATTTTTGTAGCCCGTA‑AATAAAAATTACCGGT‑TTTGGG‑Agggcg                                                                                                                           >  2:624984/1‑236 (MQ=255)                                                                                                    tACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGGTCTTCGCGTGTA‑GAACGGGGGCTCCGCGAG‑CCGCCACATCCCTCGGGGGGATTAACCGGTTTTTGTATCCCTTA‑AATAAATTTTTACTGT‑TTTTGC‑AGGccacca                                                                                                                        >  1:368106/1‑237 (MQ=255)                                                                                                    tACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGGGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCCCGCGCTCGGCGGGCA‑GGACGGGTGCTCAGCGGC‑GCCCACCCTACCTCCGGGGATTTAACCGGTGTTTTGATCCCTTACAATA‑ATTTTACCGGTGTTTggtgggctacaca                                                                                                                        >  2:963989/1‑228 (MQ=255)                                                                                                              aaaGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGGCCGCCGTTTTCGCCGGTA‑GAACCGGTGGTCAGCGGG‑CCGCATACTCCCCCCGGGGGATTAAACGGTTTTTTTATCCGTTA‑AATACATTTTAACCGT‑TTTTGG‑AAGCCAGCAAatttttggggt                                                                                                            >  2:591246/1‑232 (MQ=255)                                                                                                              aaaGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGGGTT‑GAAAGGGTGGTCCGCGGG‑CGCCCTCCACCCTCGGGGGGATTTACCCCATTTGCTATCCCCTA‑GATAACATTTACCGGG‑TTTggatgggccccacattttgtggct                                                                                                             >  2:1247163/1‑217 (MQ=255)                                                                                                                                ggTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGGTCTGCGCGTGTA‑GAACGGGGGCTCAGCGAG‑CCGCATCATCCCTCGGGGGGATTAACCGATTTTTGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGCAAGGCCAGCAAATTTGGGGGCTTGCTTAGCCGGGCCtt                                                                                            >  1:536407/1‑238 (MQ=255)                                                                                                                                         aaaaTTTTGACGGTGGCGGGTGGGAAGTGGTTGCTGGGGGGGAAGAAGGGCGGGGGAAAAAATTAAGCCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGGGTGTA‑AAACGGTTGCTCAGCGAG‑CCGCATCATCCCTCGGGTGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGt                                                                                 <  2:906926/239‑1 (MQ=255)                                                                                                                                         aaaaTCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGt                                                                                 <  1:859797/239‑1 (MQ=255)                                                                                                                                         aaaaTCTGGACGGGGGCGGCGTGGAAGTGGTTGCTTGCGGTGAAGAGGGGCAGGTGGAAAAATTAATGCAGGGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGt                                                                                 <  1:340488/239‑1 (MQ=255)                                                                                                                                          aattttgagcGATGGAAGGGGGGAGGTGTTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATGAAGGCAGTGGTAAAAGAGGG‑GC‑GGCCCGGGTTCTGCGCGTGTG‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  2:368106/230‑1 (MQ=255)                                                                                                                                          aattcttggctttggactaggggAAGTGGTTGGGTGTGGTGAAGAAGGGACGGTGGAAAAATTAATGTGGGGGTAAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGTTGCTCCGCGAG‑CCGTATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  2:823460/218‑1 (MQ=255)                                                                                                                                          aaattgtGACGGTGGCAGGGTGGAAGTGGTTGCTTGGGGGGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCGGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:591246/233‑1 (MQ=255)                                                                                                                                          aaaTTTTGGCGGTGGACGGGTGGAGGTGGTTGTTTGGGGTGAAGAAGGGCAGGTGGAAAAATTAAGGAATGGGTTAAAGAGTG‑GC‑GGCCCGCTTTCTGGGGGTTTA‑GACCGGGGGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  2:335284/239‑1 (MQ=255)                                                                                                                                          aaaTTTTGACGGTGGGAGCGTTGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAAGGCAGGTGTTAAAGAGGG‑GC‑GGCCCGTGTTCTGGGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:624984/239‑1 (MQ=255)                                                                                                                                          aaaTCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTAAAGAAGGGCAGGTGGAAAAATTAAGGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:907540/239‑1 (MQ=255)                                                                                                                                           tgtgtagaagagaccggcttGGAAGTGGTTGCTTGCGGTGAAAAAGGGCAGGTGGAAAAATTAATTCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:988285/219‑1 (MQ=255)                                                                                                                                                      tggaggggTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCATTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAg                                                                     <  1:1195444/231‑1 (MQ=255)                                                                                                                                                       tgaggggTGGAGGGGTTTGGTTGGGGTGAAGAAGGGCAGGTGGAAAATTTAATGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑AAACGGGTGCTAAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGc                                                                    <  2:61223/232‑1 (MQ=255)                                                                                                                                                                      gTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCCGTCTGCGCGTGTAGGAACGGG‑GCTCAACGAG‑CGGCAACATCCCCCCGGGGGGTTAACCGAGTTTCGTAGTCGGTA‑AATACATTTTAACGGG‑TTTGGG‑CGGACCGCAATTTTTGGGGGTTTGTTTGCCGGGGCTTTTGGTAAAAAGCGGGATAAATAACgttcctttttataaa                                                     >  2:988285/1‑224 (MQ=255)                                                                                                                                                                                 tGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCGCAGCGGG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTa                                                                                                                                              <  1:1230595/140‑1 (MQ=255)                                                                                                                                                                                   aagaagGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                <  2:314652/121‑1 (MQ=255)                                                                                                                                                                                   aagaagGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGAGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTAt                                                                                                                                                                >  1:314652/1‑121 (MQ=255)                                                                                                                                                                                           gaggtgaaaaaattatgtgtggtgTTAAAGGGGG‑GC‑GGCCCGGTTTCTGGGGGTGGA‑GAACGGGTGCCCAG‑GAGTCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                <  2:1317053/215‑1 (MQ=255)                                                                                                                                                                                           cgggTGGAAAATTAAGTGAAGGGGTT‑AAGGGTGTGC‑GGCCCGGTTTCTGCGGGTTTA‑GAACCGGGCGTCGTCGTG‑TCGGATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                <  2:892202/236‑1 (MQ=255)                                                                                                                                                                                                      aTTAAGGCATGGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCttt                         <  1:679387/234‑1 (MQ=255)                                                                                                                                                                                                           tGCAGTGGTTAAAGAGGG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCCCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                <  1:1279688/222‑1 (MQ=255)                                                                                                                                                                                                                tGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGGG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCACTTTTGGTGGCTTTCTTTGCCGGCCCTTTCTGTAAACGACGGTGTAAAAAACGGCGGTTACCCTTTTTTTTTCCCAAATAATATTCCATCGCCTTTACCAGcccaactc           >  2:679387/1‑231 (MQ=255)                                                                                                                                                                                                                  gTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTc                                                                                <  1:683677/167‑1 (MQ=255)                                                                                                                                                                                                                     aaaGAGGG‑GG‑GGCCCGCGTTCTGCGCGGGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCg     <  1:749315/239‑1 (MQ=255)                                                                                                                                                                                                                          ggg‑gc‑ggcCCGCGTTTGGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag  <  1:76248/235‑1 (MQ=255)                                                                                                                                                                                                                          gTG‑GC‑GGCCCGTGTTTCGCGCGTGTA‑GAACGAGTGCCCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTTGCTTGCTTAGCCGGCCCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag  <  1:150513/237‑1 (MQ=255)                                                                                                                                                                                                                          gTG‑GC‑GGCCCGCGTTCTGGGCGTGTA‑GAACGGGTGCTAAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag  <  1:737342/237‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                   CGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGTGGAAAAATTAATGCAGTGGTTAAAGAGTG‑GC‑GGCCCGCGTTCTGCGCGTGTA‑GAACGGGTGCTCAGCGAG‑CCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTA‑AATACATTTTACCGGT‑TTTGGC‑AGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAG  >  NC_000913/1030037‑1030490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑