Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,525,749	G→A	12.3%	L78L (CTG→TTG)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,749	0	G	A	12.3%	165.9 / 5.2	69	L78L (CTG→TTG)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base G (57/3);  new base A (2/7);  total (59/10)
Fisher's exact test for biased strand distribution p-value = 3.67e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCT  >  NC_000913/1525530‑1525986                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      cAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCCCCCAAT‑GCACGCAGACTAAAAAA‑CGAAAACa‑a                                                                                                                                                                                                                                   >  1:1250478/1‑238 (MQ=255)         gcAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAACCTCACGGGCATAAGAAACAAAACAGCC‑ACTGGAAGGGCGGGTTAAACCAAGCTCACCCAAT‑GCACCCaaaaaaaaaaa‑aaaaaaaa‑aacacaaaaa                                                                                                                                                                                                                          >  2:639568/1‑230 (MQ=255)             aGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTCCATCCAGCCAGCAACATAAGCGGCTTTCT‑CCACCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAAGGCGGGGTAGCCCCACCTCAACCAATGGCAC‑CAGAAAAAAAAA‑CGAAAAAA‑AACAAAA‑AAAtaaa                                                                                                                                                                                                                     >  2:1032659/1‑239 (MQ=255)                  cACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑c                                                                                                                                                                                                              >  1:253251/1‑239 (MQ=255)                     tGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAACA‑AAA‑ccag                                                                                                                                                                                                           >  1:855660/1‑239 (MQ=255)                        ggccccccacaaaccGCACCCCAGTAAGGCATATCAAGAGCGAGGGT‑GGTAATATCAACAATCCCCGTCAT‑CCCCCAGTTGATGAAGTACACCCAGCCAGCAAAAAAAGGGGCTTCCT‑CACCCAAAACCTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCTCCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑ccagtg                                                                                                                                                                                                         <  1:639568/224‑1 (MQ=255)                                                      cataacaaggggACGGCGGGAAATAAAAACAACCCCCG‑CATCCCCCCAGTTGAGAAAGTACACCCAGCCAGCAAAATAGGCGGCTTTCC‑CACCAAAAACCCCCCGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑Cg                                                                                                                                                                           <  1:1265168/226‑1 (MQ=255)                                                       tataCAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGc                                                                                                                                                                        >  1:931241/1‑238 (MQ=255)                                                       tataCAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CAAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGc                                                                                                                                                                        >  1:290895/1‑238 (MQ=255)                                                       tataCAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGGGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AAATAAAT‑AAA‑CCAGACCCAATGGGGGGCCCGCCAttacaaacgcggc                                                                                                                                                                          >  2:1330369/1‑227 (MQ=255)                                                              atcGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGCTGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAACGGCGGCGTAGCCCCAGCGCCCCCCAT‑GCACGCCAAATCAAAAA‑CGAAAAAA‑ACCCACA‑AATTAACA‑AAA‑CAAGTGCCATACCAGGCCACGCCAattcaaggcggtgacccgcac                                                                                                                                                                  >  2:253006/3‑221 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGCATAAAAAA‑CGCAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCAAGTGCGGGCCCCGCACTTT‑GCAAA‑CGGGCTCCTGCaa                                                                                                                                                                >  2:299045/1‑238 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGcc                                                                                                                                                                                                                                >  1:1151809/1‑180 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGcc                                                                                                                                                                 >  1:583682/1‑237 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTCCGGGCCCCGCCATTT‑GCAGT‑c                                                                                                                                                                            >  1:908796/1‑227 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAATGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCa                                                                                                                                                                 >  1:1201541/1‑238 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AACCACA‑AATTAAAT‑AAA‑ACAGTGCCACGGCGGGCCCCGCCATTT‑GCAGT‑CGGGCCCCCGCa                                                                                                                                                                 >  2:1066697/1‑238 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAAT‑AAAAA‑CGGAAACA‑AAGCCCC‑AATTAAATAAAA‑CAAGGGCCAGTCGGGGCCCCCCCttttcaagcggggcccttcac                                                                                                                                                                   >  1:77783/1‑221 (MQ=255)                                                              agCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAACGGCTTTCT‑CACCCAAAAACTCACGGGCCTAAGAAACAAAACTTCC‑ACTGGAAGGGCGGTGTAGCACCAGCCCACCCAAT‑TCACGCCAAATAAAAAA‑CGAAAACA‑A‑ACACACAATTAAAA‑AAA‑CCCGTGCCAAGCCGGGGCCCGGCAATT‑CCaagaggggccccaacc                                                                                                                                                                 >  2:509781/1‑224 (MQ=255)                                                               gCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGACTAAAAAA‑CCAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGGGCCAGTGCGGGACCCGCCCTTT‑GCAGT‑CGGGCCCCTTCAcc                                                                                                                                                               >  2:381469/1‑239 (MQ=255)                                                               gCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGGGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCAcc                                                                                                                                                               >  1:358885/1‑239 (MQ=255)                                                                cGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCCGCTCACCCCAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑CCAGT‑CGGGccctcgccacc                                                                                                                                                              >  2:651451/1‑229 (MQ=255)                                                                cGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGGGTACCACCAGCTCACCCAAT‑GCCCCCAGCATAAAAAA‑CGAAAACA‑AGCCCCA‑AATTAAAT‑AAA‑CAAATGCCCGTGCGGGCCCCCCCATTT‑GCAAC‑CGGGCCCCTgaccct                                                                                                                                                              >  2:1057201/1‑234 (MQ=255)                                                                   cggc‑ggTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAAT‑CGGGCTCCTGCACCTaaa                                                                                                                                                           >  1:1305060/1‑239 (MQ=255)                                                                         gTAATATCAACAATCCCCGTCAT‑CTCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCAAGGACATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACCAAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAACCCAGT‑CCAGGCAGGGCACCCCCATAT‑GCAGT‑CCGGCTCCTGCACCACAaaaaaa                                                                                                                                                      >  2:1188138/1‑239 (MQ=255)                                                                         gTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATtaaat‑aaa                                                                                                                                                                                                                >  1:1059258/1‑184 (MQ=255)                                                                         gTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGGGTACCACCAGCTCACCCAAT‑GCACGCCGCATAAAAAA‑CGAAAACA‑AGCCAAA‑ACTTACAT‑AAA‑CCAGTGCCCGTGCGGGCCCCGCCATTT‑GCAGTACGGGC‑CCTTCACCTAAAAACaa                                                                                                                                                      >  1:656624/1‑239 (MQ=255)                                                                                 aacaaTCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAACCCGTg                                                                                                                                               >  1:419193/1‑238 (MQ=255)                                                                                 aacaaTCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAAGGCGGGGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCAATT‑GCAGG‑CGGGCACCTGCACCTTAAAACAAAACGGTg                                                                                                                                               >  1:765317/1‑238 (MQ=255)                                                                                         ccGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATACAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGAGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAACCCGGAGCCAATcgc                                                                                                                                       >  1:839942/1‑238 (MQ=255)                                                                                           gTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCgcgc                                                                                                                                     >  1:302954/1‑238 (MQ=255)                                                                                           gTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACCCAGAATAAAAAA‑CGACAACA‑AGCCACA‑AATTAACT‑AAA‑CCATGGCCAGTGGGGGCCCCGCCATTT‑TCCAC‑CGGGGTCCTGCCCCCAAAAACAAGCCGGTGCCaacccgcc                                                                                                                                     >  2:823800/1‑232 (MQ=255)                                                                                                 cGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GAAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCCCGCCGCCaa                                                                                                                               >  1:1330828/1‑239 (MQ=255)                                                                                                      gttagaaacaGTACACCCACCCAAAAAAAAAGGGGGCTTTCC‑CCCCAAAAACCCCGCGGAAAAAAGAAAAAAAACTGCC‑ACTGGAAGGGCGGTGTAGCCACAGCTCACCAAAT‑GCACGCAAAATAAAAAA‑CAAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCg                                                                                                                          <  1:365222/230‑1 (MQ=255)                                                                                                        ttGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCgt                                                                                                                         >  1:721164/1‑237 (MQ=255)                                                                                                        ttGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCCCCTGCACCTAAAAACAAGCCGGGGCCAAACGCGCCGCCAATGGCGa                                                                                                                         >  2:393182/1‑238 (MQ=255)                                                                                                         tgagaaaGTACACCGAGCCAGAAACAAAGGCGCTTTTCC‑CCCCAAAAACCACCGGGGAAAAAAAAACAAACCGGCC‑ATTGAAAGGGCGGGTAAGCCCCACCCCCCCAAAG‑GCCCGAAAAAAAAAAAA‑CAAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCg                                                                                                                          <  1:1066697/231‑1 (MQ=255)                                                                                                                  aCATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTACAAACAAGCCGGTGCCAATCGCGCCGCCACTGGCCATACTCTTCAc                                                                                                               >  1:864698/1‑238 (MQ=255)                                                                                                                     tCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTTAAAACAACCCGTTGCCAATCGCGCCGCCAATGGCGATCATCCGCACCTgg                                                                                                           >  1:1127780/1‑239 (MQ=255)                                                                                                                     tCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTg                                                                                                            >  1:144208/1‑238 (MQ=255)                                                                                                                          ccagcAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCACTTGCACCTGGCGAt                                                                                                       >  1:874712/1‑238 (MQ=255)                                                                                                                             gcAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGCAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCCCCTAACAACAAGCCGGTGCCCATCGCGCCGCCACTGGCGATCATCtcccccagcgcattcc                                                                                                    >  2:865568/1‑223 (MQ=255)                                                                                                                                      tccgCTTTCTCCCCCCAAAACCACGAGGGCAAAAAAAAAAAACCCGCCAAGTGGAGGGGCGGTGAACCACCGGCTCACACATC‑GCACGC‑GAATAAAAAAGCAAAACCA‑AGCCACA‑AATTAAAT‑AAC‑CCGGTCCCAGGGCGGCCCCCGCCATTT‑GCAGT‑CGGCCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt                                                                                            <  2:765317/236‑1 (MQ=255)                                                                                                                                              ct‑cACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAc                                                                                   >  1:384364/1‑238 (MQ=255)                                                                                                                                                         accccccGGACATAAAAAAAAAAACCGCC‑AAGGGAAGGGGGGTGTAGCCCCAGCTCACCAAAT‑GCACGCAAAAAAAAAAA‑CGAAAACA‑AGCCAAA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGCCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTg                                                                        <  1:509781/233‑1 (MQ=255)                                                                                                                                                              aCGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTACAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCCTTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTTCCAATCCCGCCGCCCATGGCGATCAACTGACCCTTGCCATTTCCCATCCCTTTTTGATACCCCCCTTCGTGGGcct                                                                   >  2:1243275/1‑237 (MQ=255)                                                                                                                                                              aCGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGGGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGCTCATCTGCACCTGGCGATTGCCCACCGCCTTGTG‑TAccccccctccccggggct                                                                  >  2:827463/1‑225 (MQ=255)                                                                                                                                                                              aaaCTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGCGCCAACCGCGCCGCCAATGGCGATCATCCGCACCTGGCGATTGCCCATCCCTTTGGGTAACCCCCCTTCGCGGGCCTTAAGCCACCGCGGttt                                                   >  2:207335/1‑239 (MQ=255)                                                                                                                                                                                   gcacagaggAAGGGGGGTTTAGCACAGCCTCCCCAAAT‑GCCCGCAAAAAAAAAAA‑CAAAACAA‑AGCCACA‑AATTAAAA‑AAA‑CCAGTGCCAGGGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTcgcg                                               <  1:173992/232‑1 (MQ=255)                                                                                                                                                                                   gCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCCCCTGGCAATTGCCCAACGCTTTGTGAAACCCCTCTTCCTGGGCCTTAAACCAGCGGCGTTTCgcg‑g                                             >  2:121913/1‑239 (MQ=255)                                                                                                                                                                                               ggCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCAACGCTTTGGGAAACCCCCCTTCGGGGGCATTAAGCCAGCGGCGTTTCCCG‑GGGGGTTGAttt                                  >  2:1211577/1‑237 (MQ=255)                                                                                                                                                                                                gCGGTGTAGC‑CCACGCCACCCAAT‑GGCCCCAAAAAAAAAAACCGAAACAA‑AGCCAAA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATc                                   <  1:1057201/237‑1 (MQ=255)                                                                                                                                                                                                      tAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGCTACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCTTGTTGATCTGACgtgt                           >  1:1144150/1‑239 (MQ=255)                                                                                                                                                                                                            cAGCTCACCCAAT‑GCACGCAGAATAAAAAC‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCAAGCGGCGTTTACCG‑GCGAGGTGATCTGAAgcggcggtg                      >  1:445683/1‑238 (MQ=255)                                                                                                                                                                                                            cAGCTCACCAAGT‑GCACGAAAAAAAAAAAA‑CAAAAACA‑AGCCACA‑AATTAAAT‑AAC‑CCAGGCCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATCTGAAgtgtcggtgt                     <  1:393182/239‑1 (MQ=255)                                                                                                                                                                                                             accccacccaaatccccGCAAAATAAAAAA‑CGAAAACA‑GGCCCAA‑AATTAAAA‑AAA‑CCTGTCCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATCTGAAgtgtcggtgt                     <  1:299045/223‑1 (MQ=255)                                                                                                                                                                                                              gCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTACAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCCACTAAAAACAAGACGGTGCCAATCGCGCCGCCCATGGCGCTCACCTTCACCTGGCCACTCCCCATTGCTTTGGGCAACCCCTCTTAGTGGGCATTAAGCCAGCGTCGTTTCCCG‑GCGTGTTgtttgccagtgtgggggtgg                   >  2:1088927/1‑220 (MQ=255)                                                                                                                                                                                                              gCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCTTGGGCATTAAGCCAGCGGGGTTTCGCG‑GCGCGTTGATCTGAAgtgccggtgtcg                   >  1:770485/1‑239 (MQ=255)                                                                                                                                                                                                              gCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATCTGAAgcgtcggtgtcg                   >  1:848312/1‑239 (MQ=255)                                                                                                                                                                                                              gCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCCTGGGCATTAAGCCAGCGGGGTTTCGCG‑GCGTGTTGATCTGAAgggtcggggggg                   >  2:806347/1‑234 (MQ=255)                                                                                                                                                                                                              gCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCCCTTTGTGATACCCCTCTCCCTGGGCATTAAGCCCGCGGCGTTTCGCG‑GCGGGttttcttgaaagtggggggtcg                   >  2:572402/1‑219 (MQ=255)                                                                                                                                                                                                              gCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCACCTGCACCTGCCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCCTTAAGCCAGGGGCGTTTCGCG‑GGGTGTGGTTCTGAAaggtcggggtgg                   >  2:1371372/1‑237 (MQ=255)                                                                                                                                                                                                                    ccAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATCTGAAGTGCCGGTGTCGGGttt              >  1:228987/1‑238 (MQ=255)                                                                                                                                                                                                                        t‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAGGCCAGCGGCGTTTCCCG‑GGGTGTTGATCTGAAGTGTCGGCGGGGGGTTTACTCa         >  2:875307/1‑239 (MQ=255)                                                                                                                                                                                                                        t‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATCTGAAGGGTCGGGGTCGTGTTTACTCa         >  1:446445/1‑239 (MQ=255)                                                                                                                                                                                                                          gCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCCTTAAGCCAGCGGCGTTTCGCG‑GGGTGTTGTTCTGAAGGGTCGGGGTGGGGttttcccgt        >  2:736729/1‑233 (MQ=255)                                                                                                                                                                                                                          gCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAC‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAACGGCGTTTCGCG‑GCGGGGTATTCTTAAGTGTCGGGTGCGTGGTTACTTAt        >  1:643791/1‑239 (MQ=255)                                                                                                                                                                                                                             cGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTTCCCATCGCTTTGTGATACCCCCCTTCGTGGGCATTAACCCAGCGGCGTTTCGCG‑GCGTTTTGTTCTGAAGTTGCGGTGTGGTGGTTTCCCAtgt      >  2:706348/1‑236 (MQ=255)                                                                                                                                                                                                                               cAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGCTTGCCCATCGCTTTGTGATACCCCTCTCCGCGGGCATTAAGCCAGCGGCGTTTCCCGCGCGT‑TTGATCTTAAAGGTGGGCGGCGTGTTTAcccctttct    >  2:198977/1‑230 (MQ=255)                                                                                                                                                                                                                               cAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGGGTTTCCCG‑GCTGGTTGTTTTGAAGGGTGAGTGTCGGGTTTAACCATTggc    >  2:427906/1‑236 (MQ=255)                                                                                                                                                                                                                                 gAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCCCCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATGAACCCATGGCCGTTTCGCC‑GGGGGTTGAGTTGGAGGGTCGGGTTGGGGTTTACACAtttcctc  >  2:596905/1‑233 (MQ=255)                                                                                                                                                                                                                                 gAATAAAAAA‑AAAAAACACACCCCGA‑AAGGAAAA‑AAA‑CCAG‑GGCAGGGGGGGGCCCCCCATTTGGCAGT‑GGGGCACCGGCACATAAAAACAATCCGGTGCCAATCGGGCCGCCAATGGCGATCACCTGCACCTGGCGATTGCCCATCGCCTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGCGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGcgc   <  2:864698/238‑3 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      CAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC‑GGTAATATCAACAATCCCCGTCAT‑CGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCT‑CACCCAAAAACTCACGGGCATAAGAAACAAAACTGCC‑ACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAT‑GCACGCAGAATAAAAAA‑CGAAAACA‑AGCCACA‑AATTAAAT‑AAA‑CCAGTGCCAGTGCGGGCCCCGCCATTT‑GCAGT‑CGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCG‑GCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCT  >  NC_000913/1525530‑1525986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑