BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,872,595	A→G	15.1%	L75P (CTC→CCC)	ftsB ←	cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,872,595	0	A	G	15.1%	203.9 / 3.6	94	L75P (CTC→CCC)	ftsB	cell division protein
Reads supporting (aligned to +/- strand): ref base A (32/43); new base G (8/8); total (42/52)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGA  >  NC_000913/2872359‑2872830

cATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGGGt                                                                                                                                                                                                                                              >  1:516306/1‑239 (MQ=255)

         aGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCAGTTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTTCCCCACA‑GACTGGGGGCGCC‑T‑CGACG‑GCTCAGGCCCCAGA‑CGAAAAAAAGGTTTGCCC‑GGCGTGGGAATGCTATGTTCATTaac                                                                                                                                                                                                                                      >  2:857163/1‑236 (MQ=255)

            ttttaaaagtGGTTTGATTACCGATTGAGGGATTTTGCTTAGGACATTCCGTTTGCAGTCCGCGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑Ca                                                                                                                                                                                                                                 <  2:368156/230‑1 (MQ=255)

            gtgcAAGAATGGTTTGATTTCCGATTGAGAGATATTGCTTAAGACATTCCGTTTGCATGCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTG‑CCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑Ca                                                                                                                                                                                                                                 <  1:790538/235‑1 (MQ=255)

            gtTTAAGAGTGGTTTGTTTGCCGATTGAGAGATTTTGCTTAGGACATTCCTTTTGCATTCGCCGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAATTGAGTGT‑TTGCCAGGTTAATTCCCGGGCTG‑ATTTATCGAGTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑Ca                                                                                                                                                                                                                                 <  2:724849/239‑1 (MQ=255)

            gtTCAAGAATGGTTTGATTGCCGATTGAGAGATATTTCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑Ca                                                                                                                                                                                                                                 <  1:902226/239‑1 (MQ=255)

            gtTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑Ca                                                                                                                                                                                                                                 <  1:628819/239‑1 (MQ=255)

            gtTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑Ca                                                                                                                                                                                                                                 <  1:838856/239‑1 (MQ=255)

             ttCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GCCTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGTAAAAAAGTTTCGCCC‑GGCCTGGTCAAGTTGAGTTCATTACGCG‑CAc                                                                                                                                                                                                                                >  2:76835/1‑239 (MQ=255)

             ttCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GCCTGTGCGCGCT‑T‑CGACG‑CGTCAGGACCCAAA‑CGGTAAAAAGTTTCGCCC‑GGCCTGGTCAGGCTCGGTTAATTACacg‑cc                                                                                                                                                                                                                                 >  2:141507/1‑237 (MQ=255)

              tccaGATTGTTTTGATTGCCGATTGAGAGATTTGTCTTAGGGGATTCCGTTTGAAATTGATGGCCAAACCCGGACGCCGGAACCACGGCGAAAAATTCCAAAGGTGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGC‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACg                                                                                                                                                                                                                               <  2:959312/236‑1 (MQ=255)

                       gTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGGGTTCATTACGCG‑CACGCCCTTCggg                                                                                                                                                                                                                      >  2:183039/1‑237 (MQ=255)

                       gTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGGGGTCATTCCGCC‑CACGCTCTTCGa                                                                                                                                                                                                                       >  1:1078423/1‑238 (MQ=255)

                          tGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACCCCTTTCGAggc                                                                                                                                                                                                                    >  1:418130/1‑236 (MQ=255)

                               aCCGCTTGAGAGTTTTTGGTTAGGACATTCCGTTTCACTGTGCCGGCCAAACCCGTCCCCCGGAACCCCGGCGCAAACATCCAAATGAGTGG‑TTGCCATTTTAATTCCCGGGCGG‑ATTTATCGATTGGTTTGCCCCGCA‑GATTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGcctcct                                                                                                                                                                                                              <  2:755471/239‑1 (MQ=255)

                                 cgtttgagcatttttcgttAGGCCCTTCCGTTGGCATCCGAGGGCAAAATCCGGCCCCCGGAACCACGGCCCAAACCTCCAAATTAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATGGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTgg                                                                                                                                                                                                            <  2:396022/222‑1 (MQ=255)

                                              ttGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCCCCAGA‑CGGTAAAAAGTTTCGCCC‑GGCCCGGTCAAGCTGGGTTCATTTCGCG‑CACCCGCTTCGAGCGCCCCCTGGC‑CCCCCTTggggt                                                                                                                                                                                              >  2:566384/1‑235 (MQ=255)

                                                 cTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATCAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACCCG‑CACGCCCTTCGAGCGACCCCGGGC‑CCCCCttagaaccgc                                                                                                                                                                                           >  2:195318/1‑231 (MQ=255)

                                                   tAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGGGCGCCTCCTGGC‑CGCCATTGGGATCGCCa                                                                                                                                                                                         >  1:543901/1‑239 (MQ=255)

                                                     ggACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTAAGGCACCAGA‑CGATAAAAAGGTTCGCCC‑GGCCTGGTCAGGCTGGGTTCATTACGGC‑CACGCCATTCGAGCGCCCCCTGGC‑CGCCATTGAGATCGGCAAt                                                                                                                                                                                       >  2:365359/1‑239 (MQ=255)

                                                      gACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGGGTTCATTACGCG‑CACCCTCTTCGAGCGCCCCCTGGC‑CGCCATTGAGATCGCAAAtt                                                                                                                                                                                      >  2:591969/1‑239 (MQ=255)

                                                      gACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGCGATCGTCAAtt                                                                                                                                                                                      >  1:933461/1‑239 (MQ=255)

                                                           tCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGCCG‑CGTCAGGCCCACAA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCCTGCTGCGTTCATTAACCG‑CACCCCCTTCGAGCGCCTCCTGTC‑CCCCATTGCGATCGTCAATTTcccc                                                                                                                                                                                 >  2:692766/1‑236 (MQ=255)

                                                           tCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAAATGGTCAATTTCGGc                                                                                                                                                                                 >  1:258359/1‑239 (MQ=255)

                                                           tCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCCCCTGGC‑CGCCATTTAGATCGTCAATTTCGGc                                                                                                                                                                                 >  1:1073073/1‑239 (MQ=255)

                                                           tCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTAATGCTGAGTTCATTACGCG‑CACGCCCTTCGAGCGCCCCCTGCC‑CGCCATTAAGATCGTCAATTTCGGc                                                                                                                                                                                 >  2:338197/1‑239 (MQ=255)

                                                           tCCGTTTGCATTCGACGGCCAAATCCGGCCACCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GCCTGTGCGAGCT‑T‑CGACG‑CGTCAGGAACCCAC‑CGATCAAAAGTTTCGCCC‑GGCCTTGCCAGGTTGGGTTCATTCCCGG‑CACTCTCTTCTGGCCCCT‑CTGGCACGCACTTGAGAAAGTCAAATTCGGc                                                                                                                                                                                 >  2:1029629/1‑239 (MQ=255)

                                                            ccGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCa                                                                                                                                                                                <  1:353357/239‑1 (MQ=255)

                                                                   cATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGCGCGCCACCTGGC‑CGCCATTGAATTCGTCACTTTGGGCCAAAAGtg                                                                                                                                                                         >  2:1053634/1‑238 (MQ=255)

                                                                     ctCGCCGGCCAACTCGGGCCGCCGGAACCACGGCGCAAACATCCAAATGGGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTGCGGCAAAAAGTTGa                                                                                                                                                                       <  2:464935/238‑1 (MQ=255)

                                                                             gcAAACCCGGCCCCCGGACCCCCCGCCAAAACACCCAAATGATGGG‑TTGCCCTGTTAATTCCCCGGCTG‑TTTTATCGTATGTTTTTCACCGCA‑GACCTTGCGCGCT‑T‑CGGAG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTATTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTcg                                                                                                                                                               <  2:657798/238‑1 (MQ=255)

                                                                             ccAAACCCGGCCCGCGGAACCACGGCGACAACATCCAAATGGGTGG‑TTGCCATGTTAAGTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑GGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTc                                                                                                                                                                <  1:950053/238‑1 (MQ=255)

                                                                                       ccgccgGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCATGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCTTTACCCG‑CAGGCTCTTCGAGCGCCCCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAATTGATTGTTTGGCGGTTaaatt                                                                                                                                                     >  2:685231/1‑237 (MQ=255)

                                                                                       ccgccgGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAACGTTGATCGTTTCGCGCTTTAAGt                                                                                                                                                     >  2:910746/1‑239 (MQ=255)

                                                                                         gccgGACCCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGttt                                                                                                                                                   <  1:76835/239‑1 (MQ=255)

                                                                                         gccgGAACCACGGCGCAACCATCAAAATGAGTGG‑TTGCCAGGGTAATTCCCGGGGCG‑ATTTATCGATTGTTTTGCCCCGCA‑GGCTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGttt                                                                                                                                                   <  1:1053634/239‑1 (MQ=255)

                                                                                         gccgGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGttt                                                                                                                                                   <  1:909159/239‑1 (MQ=255)

                                                                                                   cGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGGCCTTTAAGTTTCGCGTTTGTa                                                                                                                                         >  1:36703/1‑239 (MQ=255)

                                                                                                           acacCCAAAGGGGGGG‑TTTCCTGGTTAACGCCCGGGCTT‑ATTTGTCTGTGGTGTTGCCCCGCA‑GACGGTGCGCGCT‑T‑CGCCG‑GGCAAGGCACCAGA‑CGATAAAAGGTTTCGCCC‑GGCCTGGTCAGGCGGATTTAATTACGCG‑CCCGCTCTTCGAGCGCCTCTTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCtg                                                                                                                                 <  2:654323/239‑1 (MQ=255)

                                                                                                             ctCCAAAGGATTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTTCGCGCT‑T‑CGACG‑CGTCAGGCCCCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCtgtg                                                                                                                               <  1:920688/238‑1 (MQ=255)

                                                                                                                  catttggtttttcccATTTTAATTCCCGGGTCT‑ATTTATCGATTTTTTGCCCCCGCA‑GCTTGGGGGCGGT‑T‑GGGCG‑GTGCGGGCACCAGA‑CGAAAAAAAGTTCCCCCC‑GGCCTGGCCATGCTGGTTTCATTACGCG‑CCCGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTgccgc                                                                                                                           <  2:1073073/226‑1 (MQ=255)

                                                                                                                  aattggggtgtgccccttttAGTTCCC‑GGCTGTATTTCACGTTTGTTTTGCCCCGCA‑GCATGTGCGCGCT‑T‑CGACG‑CGTCGGGCACCAGA‑CGAAAAAAAGTTGCCCCC‑GCCCTGGTCATGCTGGGTTCATTACGCG‑CACGCTCGTCAGGCGCTTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTgccgc                                                                                                                           <  2:292499/221‑1 (MQ=255)

                                                                                                                  aaTGGGTGGTTTGCATTGTTAGTTCCGGGCTTG‑ATTTACTGATT‑TTTTGCCCCCCAGGACTGGGCG‑GCTCT‑CCACG‑TGCCAGGACCCAGA‑CGATAAAAAGTTTCGCCC‑GGCCGGGGCATGCGTGGTTCATTCCGCG‑CACGCCTCTCGAGCCCTTCCGGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATAGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTgccgc                                                                                                                           <  2:836969/239‑1 (MQ=255)

                                                                                                                      ggTGG‑TTGCCTTTTTAATTCCCGGGCGT‑ATTTACGGGTTTTTTGGCCCAGCG‑GATTGTGCGCGCT‑T‑GGTCG‑GGCAAGGCCCCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CCCGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTGGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGATGTGCCGCCAcat                                                                                                                      <  2:704048/238‑1 (MQ=255)

                                                                                                                      aGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCCTTGAGATCGTCAATTTCGGCCAAAAGTTGATCGTTTCCGCCTTTAAGTTTGCGGTTTTTAGCTTGTGGTgccgcccaa                                                                                                                       >  2:595000/1‑235 (MQ=255)

                                                                                                                            ttGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAACAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGGGCCGACAcatcatca                                                                                                                 >  1:292499/1‑239 (MQ=255)

                                                                                                                              gCCGTGTTAATTCCCGGGGGG‑TTTTTACATTTGTTTTCCCCCGGA‑GATTGGGCGGCCT‑T‑CGACG‑CGGCAGGCACCGAA‑AGAAAAAAGGTTCCGCCC‑GGCCTGGTCAGTCGGAGTTCATTCCGCG‑CCCGCTCTTCGAGCGCCTCCTGGC‑CGCCATGGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCAtt                                                                                                               <  2:36703/239‑1 (MQ=255)

                                                                                                                              gCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAGAAGTTGATCGTTTCGCGCTTTAAGTTTCCAGTTGTTAGCTTGCTGTGCGCCCCCATCATCAtt                                                                                                               >  1:819074/1‑239 (MQ=255)

                                                                                                                                 aTGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAGGTTTCGCGTTTGTAGCTTCCTGTGCCGCCAAACAATCATTTAc                                                                                                            >  2:150231/1‑239 (MQ=255)

                                                                                                                                  ttttAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAc‑g                                                                                                          <  1:703634/237‑1 (MQ=255)

                                                                                                                                  tGTTAATTCCCGGGGTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAc‑g                                                                                                          <  1:150231/239‑1 (MQ=255)

                                                                                                                                  tGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGGGCGCGCT‑T‑CGAGG‑CGTCAGGCACAAGA‑CGATAAAAGGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAc‑g                                                                                                          <  1:141507/239‑1 (MQ=255)

                                                                                                                                   gTTAATTCCCGGGCTG‑ATTTAACGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAc‑gc                                                                                                         <  1:183039/239‑1 (MQ=255)

                                                                                                                                      aaTTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCggg                                                                                                      >  1:387520/1‑239 (MQ=255)

                                                                                                                                        ttCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGta                                                                                                    <  1:839200/239‑1 (MQ=255)

                                                                                                                                        ttCCCGGGCTG‑ATTTACCGATTGTTTTGCCCCGCA‑GACTGGGGGCGCT‑T‑CGGCG‑CGTCAGGCCCCAGG‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCCCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGta                                                                                                    <  1:338197/239‑1 (MQ=255)

                                                                                                                                        ttCCCGGGCGT‑TTTTGTCGTTTGTTTTGCCCCCCA‑‑AGTGTGCCCCCT‑T‑TGGCGCCGGCAGGCACCAGA‑CGAAAAAAAGTTTCGCCC‑GCCCTGGTCATGCTGAGTTCATTACGCC‑CCCCCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGta                                                                                                    <  2:933461/239‑1 (MQ=255)

                                                                                                                                         ttccggggtgttttttccgttttttttCCCCCGCA‑GATTGTGCGCCGT‑T‑CG‑CG‑CCTAGGGGCCCAGACCAATAAAAAGTTCGCCCC‑GCCCGGGTCTTGCTGGGTTCATTCCGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGta                                                                                                    <  2:543901/216‑1 (MQ=255)

                                                                                                                                         aaagggagcggttttttcgttgtttttGCCCCGAA‑GCTTGGGCGGGTT‑T‑CGAGG‑GGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGta                                                                                                    <  2:587490/216‑1 (MQ=255)

                                                                                                                                          cccGGGCTG‑GTTTACCAGTTGTTTGCCCCCGCA‑GACTGGGCCGCCT‑T‑CGACC‑GGTCAGGCCCCGGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTGCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGtata                                                                                                  <  1:365359/239‑1 (MQ=255)

                                                                                                                                          cccGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGtata                                                                                                  <  1:217958/239‑1 (MQ=255)

                                                                                                                                          cccGGGCGG‑ATTTATCGTTTGTTTTGCCCCGCA‑GCCTGTGCGCCCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGtata                                                                                                  <  1:31132/239‑1 (MQ=255)

                                                                                                                                           ccgggtgttttttccgcttttttttCCCCCGCG‑GACTGGGCGCCTT‑C‑CGCCG‑GGTCAGGCACCAGA‑CGAAAAAAATTTCCGCCC‑TGCCTGGCCATGCTGGGTCCATTACCCG‑CACGCTCCTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGtata                                                                                                  <  2:626700/218‑1 (MQ=255)

                                                                                                                                           cccgggcgttttgttCGTTTGTTTTGCCCGGCG‑GAC‑GTGTGCTCT‑TGTGACC‑GGTGAGGCCCCAGT‑CGATAAAAAGTTTCGCCC‑GCCCGGGTCATGTTATTTTCATTCCGCG‑CCGGCTGTTCGGGCGCCACCTGGC‑CGCCTTGGAGATCGTCAGTTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGtata                                                                                                  <  2:905437/225‑1 (MQ=255)

                                                                                                                                           cccggcttgttttttggttttttttCCCCCGCA‑GCTTGGGCGCGCT‑T‑GGAGG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCGGGTCATGCTGATTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGtata                                                                                                  <  2:1078423/218‑1 (MQ=255)

                                                                                                                                             gggCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑CCGGGTATAGta                                                                                               >  1:903034/1‑238 (MQ=255)

                                                                                                                                             cgggttttttttTCGTTTTTTTGCCCCCGGA‑GCCTGGCCGCTCT‑T‑GGCCG‑CGCGAGGCCCCAGG‑CGAAAAAAAGTTTGCCCC‑GGCCTGGGCATGCGGAGTTCATTACGCG‑CACCCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGt                                                                                                <  2:903034/231‑1 (MQ=255)

                                                                                                                                                    tttttcggATTGTTTTGCCCCGCA‑GACGTTGCCCGTT‑T‑GGGCG‑GGTCGGGCCCCAAA‑CGAAAAAAAGTTTCGCCC‑GGCCTGGTAATGTTGAGTTCATTCCGCG‑CCCGCTTTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGta                                                                                                    <  2:785772/221‑1 (MQ=255)

                                                                                                                                                     tttATCGATTGGTTTGCCCCGCA‑GGCGGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CCCGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGtata                                                                                        <  1:886758/239‑1 (MQ=255)

                                                                                                                                                              tGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTTTAGCTTGCTGTGCCGCCACACCATCTTTGCC‑GCGGGtaaatgtctttaaaacgttttt                                                                                >  2:1051622/1‑218 (MQ=255)

                                                                                                                                                              tGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTTAC‑GCGGGTATAGTCATGTATACCcttctt                                                                                >  1:454923/1‑238 (MQ=255)

                                                                                                                                                              tGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGGATAGTCATGta                                                                                          >  1:587490/1‑228 (MQ=255)

                                                                                                                                                              tGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGCATAGTCATGtatctgtctcttt                                                                               >  1:785772/1‑229 (MQ=255)

                                                                                                                                                                ttttGCCCCGCA‑GTCTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGtata                                                                                        <  1:78861/228‑1 (MQ=255)

                                                                                                                                                                ttttGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGTTGTGCCGCCCCATCATCATTGTG‑GCGGGGTTATTCTTGATAACCGTTTTTAcc                                                                             >  2:541965/1‑239 (MQ=255)

                                                                                                                                                                          cA‑GGCTGGGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGc                                                                   <  1:627956/239‑1 (MQ=255)

                                                                                                                                                                          cA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCCCGTTTGTAGCTTGCTGGTCCGCCCACTCATCATTTCC‑CGGGGCAAAGGCATTTAAACCGttttatcccggctccttt                                                                   >  2:78861/1‑223 (MQ=255)

                                                                                                                                                                               cTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGa                                                                 <  1:195318/237‑1 (MQ=255)

                                                                                                                                                                                 gtgCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACcacaacacaa                                                               >  2:318055/1‑231 (MQ=255)

                                                                                                                                                                                  tgCGCGCT‑T‑CGACG‑CGTCAGGCCCCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACt                                                            <  1:485184/239‑1 (MQ=255)

                                                                                                                                                                                    cgcgcT‑T‑CGACG‑CGTCAGGCAACAAA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTCAGTTCATTACGCG‑CACGCGCTTAGAGCGCCTCCTGGC‑CGCCATGAAGATCGTCAATTTTGGGAAAAAGCTGCTCGTTTCACTCTTTAAGCTCTGCGGTTATAGCTGGCTTTGCCGCAACCTCATCA‑TGACATCGGGTCTAGTCATGTCTACCATACTCACAGAAacaccgagacaaccgt                                                          >  2:243379/1‑227 (MQ=255)

                                                                                                                                                                                       gtt‑t‑CGACG‑CGTGAGGCACCAGA‑CGATAAAAAGTTTCCCCC‑GGCCTGGTCATGCTGGGTTCATTCCGCT‑CACGCTCTCCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGc                                                       <  1:595000/237‑1 (MQ=255)

                                                                                                                                                                                             tggcgccgtttggtccccaa‑CGAGAAAAAATTTTGCCCGGGCCTGGTCGGGTTGGGTTACTTACGCG‑CACCCCTTTCGCGGGCCCGCTGTC‑CGCCATTGAGGCTGTCAATTGCGGCAAAAAGTTGATCGTTTCGCGTTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGcca                                                     <  2:1051543/220‑1 (MQ=255)

                                                                                                                                                                                              ggagcgttCAGGCCCCAGA‑CAAAAAATAGTTCCCCCC‑GCCCTGGTCATGCTGAGTTCATTACGCG‑CCCCGCCTACGAGCGCCTCCTGCC‑CGACTTTGAGATCGTAAATTTCGGCAAAAAGTTGATCTTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTGGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGccaga                                                   <  2:235745/232‑1 (MQ=255)

                                                                                                                                                                                                              aGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACACCGAATACTGTAGCCAGACCAGAAAAGCCAGCaa                                   >  1:826294/1‑239 (MQ=255)

                                                                                                                                                                                                                  tcaaaaaacttttCCCCC‑GGCTTGGTTCTGCTGAGTTCATT‑GGCGCCCCGCTCTTCGGGCCCCTCCTGGC‑GGCAATTGAGTTCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGTTTTAAGTTTCGGGTTTGTAGTTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAAcag                                <  2:922621/229‑1 (MQ=255)

                                                                                                                                                                                                                      aaaaaGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCACCaccagc                            >  1:918005/1‑239 (MQ=255)

                                                                                                                                                                                                                       aaaaGTTCCGCCC‑GGCCTGGTCATGGTGGTTTCATTACGCG‑CACGCTTTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGAGCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCg                           <  1:14953/239‑1 (MQ=255)

                                                                                                                                                                                                                                       ccTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGGATAGTCATGTATACCGTTCTTCCCGAACCACAGCGAATACTTTAGCCAGACCAGAAAAGCACGCaaacaaaaaggaaagtacaccaccc            >  2:430564/1‑216 (MQ=255)

                                                                                                                                                                                                                                         tGGTGATGCTGAGTTCATTACGCG‑ACCGCTTTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGGCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTg          <  1:175902/239‑1 (MQ=255)

                                                                                                                                                                                                                                           gTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACATCGAATACTGTAGCCAGACAAGAATAGCCACCAACAACACCGTTAGTTTACCCCTCCTGc         >  1:482493/1‑238 (MQ=255)

                                                                                                                                                                                                                                            tCATGCTGAGTTCATTACGGG‑CACGCCCTTCGAGCGCCTCCTGGC‑CGCCATTGGGTTCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAc                                                                                                            <  2:1049358/139‑1 (MQ=255)

                                                                                                                                                                                                                                                 cTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCCCATCATCATTGAC‑GCGGGTATTGTCATGTATACCGTTCTTACCGAACCCCACCGAAAACTGTAGCCAGACCAAAATAGCCAGGAACACCCCCCTTAGTTTACCCACCCCGccccccca  >  2:305865/1‑236 (MQ=255)

                                                                                                                                                                                                                                                 cTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTAACCATCCTGCCCCCTGa  >  1:854519/1‑239 (MQ=255)

CATGCACCGAGTGTTCAAGAATGGTTTGATTACCGATTGAGAGATATTGCTTAGGACATTCCGTTTGCATTCGACGGCCAAATCCGGCCGCCGGAACCACGGCGCAAACATCCAAATGAGTGG‑TTGCCATGTTAATTCCCGGGCTG‑ATTTATCGATTGTTTTGCCCCGCA‑GACTGTGCGCGCT‑T‑CGACG‑CGTCAGGCACCAGA‑CGATAAAAAGTTTCGCCC‑GGCCTGGTCATGCTGAGTTCATTACGCG‑CACGCTCTTCGAGCGCCTCCTGGC‑CGCCATTGAGATCGTCAATTTCGGCAAAAAGTTGATCGTTTCGCGCTTTAAGTTTCGCGTTTGTAGCTTGCTGTGCCGCCACATCATCATTGAC‑GCGGGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCCATCCTGCCCCCTGA  >  NC_000913/2872359‑2872830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`