Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,154,040	T→G	13.3%	S160R (AGT→AGG)	yghB →	general envelope maintenance protein, DedA family inner membrane protein, putative multidrug efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,154,040	0	T	G	13.3%	179.5 / 3.2	84	S160R (AGT→AGG)	yghB	general envelope maintenance protein, DedA family inner membrane protein, putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (31/38);  new base G (10/2);  total (44/40)
Fisher's exact test for biased strand distribution p-value = 2.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCTGGCTAAGTTATATTCAGGGGCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT  >  NC_000913/3153805‑3154274                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              gttgttgtttatttttttttggggggCGTTGG‑TTGGGAAATACCAAAC‑G‑GGGGAAGGGCGGGCGGG‑CA‑CATCTCCCT‑GAAAAATACCCCCCGCGCGCCACCTGTTTTTT‑T‑GCCCGCCCCGGTCTGCTGGCGCTGCTGGTGGGACTTTTTCTGGCATTTGCCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTgg                                                                                                                                                                                                                                             <  2:591340/216‑1 (MQ=255) gtctgtggtattgttttttttaGGGGGGGTTG‑TTTGGGAATACCAAAACC‑GGTGAAGGGTTGTC‑GG‑CACCAGTTTCTT‑GCTAAATACCCCAGGGGCGCCACCTGAATTTT‑T‑GCCCGCCGCGGTCTGCTGGCGCTGCGGGCTGGACGTTTTCTTGCTTTTGTCCGTACGCTGCTGCCACCCATGGCGGGAATTC‑CCGGTCTGCCAATCCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTgg                                                                                                                                                                                                                                             <  2:102182/218‑1 (MQ=255)   tgtgggttAATTTATATTCGGGGGGGTTGG‑TTAGGAAATACCAAAG‑C‑GGAGAAGGGCTGGCTGG‑CA‑CAGTTTCCT‑GCTAAATATCACCAGCGCGCCCCCTGCATGTT‑T‑GCCGGCCCCGGTCTGCTGGGGTGGCTGGCTGGACGTTTTCTTGCATTTGTCCTTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑t                                                                                                                                                                                                                                          <  1:867200/231‑1 (MQ=255)   tggtgGCTAATTTATATTCAGGGGGCGTGG‑TTAGGGAATCCAAAAA‑C‑GGTGAAAGGCTGGCGGG‑CA‑CGGTTTCCT‑GCAAAATATCACCAGCGCGCCTCCTGCATGTT‑T‑GACCGCCACGGTCTGGTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑t                                                                                                                                                                                                                                          <  1:129347/235‑1 (MQ=255)    tgggggttatttttttttaGGGGGCCTGGTTTAGGAAAACCCAAAA‑G‑GGGAAAAGGGTGGGGGG‑CA‑CGTCTTCCG‑TCAAAATCTCCCCGGGCCGCCCCGTGCTTTTT‑G‑GCCCGCCCCGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCTTTTGTCCTTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑t                                                                                                                                                                                                                                          <  2:111884/220‑1 (MQ=255)     ctgGCTAAGTTATATTCAGGGGCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCCG‑TTTTTCAAC‑TGGTTAAGTGGA‑tt                                                                                                                                                                                                                                         >  1:855095/1‑237 (MQ=255)             tttttttgggggggCGCT‑G‑TTGGGGAAAACCCAAC‑C‑GGGGAAAGGGTGGGTGGTCA‑CGGCTCCGT‑TCTAATTCCTCCCGGCGCGCCCCGTG‑TTGTTGT‑GCCCCCCGCGGTCTGCGGGCGCTGCTGGCGGGCCGTTTTCTGGACTTGGCCCGTACGCTGCTGCCACCCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTc                                                                                                                                                                                                                               <  2:748314/229‑1 (MQ=255)                  atTCAGGGGCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGCCTGCCAAACCGCCGCTTCCCG‑TTTTTCAAC‑TGGTTAAGGGGA‑TTGCTGTGGGGCAGCGt                                                                                                                                                                                                                          >  1:386451/1‑239 (MQ=255)                        gggCGCTTG‑GTTGGGAATTCCCAAA‑CGGGGGAAGGGCTGGGGGG‑CC‑CGGCTTCCG‑GCAAAATCCCCCAGGCGCGCCCCCTGCATGTT‑T‑ACCCGCCACGGTCTGCGGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTaa                                                                                                                                                                                                                     <  2:931915/239‑1 (MQ=255)                        gggCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGGTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCGGCTGCCAACCATGGCGGGAATTT‑CCGCGTTGCCAAACCCGCGCTTCCCGCTTTTTCAAC‑GGGTGAAggggtgttttgggggggggggggacac                                                                                                                                                                                                                      >  2:1043603/1‑216 (MQ=255)                        gggCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTT‑CCGGTCTGCCCAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGATAAGTGGA‑TTGCTGTGGGTCAGCGGGGGaa                                                                                                                                                                                                                     >  1:1030129/1‑238 (MQ=255)                        gggCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTTCCAAACCGCCCCCTCCCG‑TTTTTTCAC‑GGGTTAAATGGA‑TTGCTTGGGGTCCACGTGGTaaa                                                                                                                                                                                                                    >  2:294580/1‑238 (MQ=255)                        gggCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCCG‑TTTTTCCAC‑TTGTTAAGGGGA‑TTTCTGTGGGTCAGCGGGGGaa                                                                                                                                                                                                                     >  1:1059795/1‑238 (MQ=255)                        gggCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCCA‑GTTTTCAAC‑TGGTTAAGTGGA‑TTTCTGTTGGTCAGCGGGGAAAc                                                                                                                                                                                                                    >  1:263860/1‑239 (MQ=255)                        gggCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCCCTTCCCG‑TTTTTTCAC‑TGGTTAAGGGGA‑TTGCTGTGGGTCAGCGGGGGAAc                                                                                                                                                                                                                    >  2:219913/1‑239 (MQ=255)                        gggCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCCCTTCCAG‑TTTTTCAAC‑TGGTTAAAGGGA‑TTGCTTTGGGGCACCGGGGTaa                                                                                                                                                                                                                     >  1:337770/1‑238 (MQ=255)                           cgcTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTT‑CCGGTCTGCCAAACCGCCGCTTCCCG‑TTTTTTAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAggctgggaaaccg                                                                                                                                                                                                                 >  1:485122/1‑227 (MQ=255)                                g‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTT‑CCGGTCTGCCACACCCCCGCCTCCCG‑GTTTTCAAC‑TGGGTTAGGGGG‑TTGGTTGGGGTCGGCCGGGTAACCCGttttt                                                                                                                                                                                                            >  2:618442/1‑238 (MQ=255)                                     gggAAACCCCAAA‑C‑GGTGAAAGGCGGGGCGG‑CC‑CCTGTTCCTGGAAAAATTCCCCCGGCGCGCCCCCTCCTTTTT‑G‑GCCCGCCGGGGTCTGCTGGCGCTGTGGGCTGGACGTTTTCTTGCTTTTGCCCGTACGCTGCTGCCACCCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCt                                                                                                                                                                                                         <  2:1066907/239‑1 (MQ=255)                                     ggaaaTCCCAAAC‑C‑GGTGAAAGGTGGGTGGG‑CA‑CGGCTTCCT‑GCAAAATTCCACAGGCGCGCCCCCGGCATGTT‑T‑GCCCGCCACGGTTTGCGGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTa                                                                                                                                                                                                        <  1:70823/236‑1 (MQ=255)                                      ggAAAACCCAAA‑CGGGTGAAGGGCGGGCTGC‑CA‑CAGTTTCCT‑GCTAAATACCCCCAGCGCGCCCCCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTa                                                                                                                                                                                                        <  1:93713/239‑1 (MQ=255)                                      gaaaTCCCAACA‑C‑GG‑GAAAGGGTTGGTGG‑CC‑CAGCTTCCTGGTTAAATTCCCCCGGGGCG‑CTCCTGGTTTTT‑TGGCCCCCCACGGTCTGCGGGCGGTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTa                                                                                                                                                                                                        <  2:510718/236‑1 (MQ=255)                                         aTACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGGCTGCCAAACCGCCCCCTCCAG‑CTTTTCAAC‑TGGTTAAGTGGAGTTGC‑GTGGGGCAGCGTGGTAAACAGGTTTTGCTAtt                                                                                                                                                                                                      >  2:156904/1‑236 (MQ=255)                                           aCCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCCAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTTCTTTgg                                                                                                                                                                                                                                  >  1:366050/1‑207 (MQ=255)                                           aCCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCCG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCCGTTTTGGCTATG‑CCtt                                                                                                                                                                                                 >  1:357590/1‑239 (MQ=255)                                           aCCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCCCCGCTTCCAG‑TTTTTCAAA‑TTGTTAAGTGGA‑TTGCTCTGGGTACACGTGGTAACCCAGTTTGGCATTG‑CCtt                                                                                                                                                                                                 >  1:125198/1‑239 (MQ=255)                                           aCCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGGT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCTTACGCTGCTGCCAACCATGGCGGGGATTT‑CCGGACTGCCAAAACGCCGCTTTCCG‑TCTTTTCAC‑TGGTTAAATGGA‑TTGCTGTGGGTCACCGGGGTAAACAGTTTTGGCAATG‑ctta                                                                                                                                                                                                 >  2:137938/1‑236 (MQ=255)                                               aaa‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGGGGG‑TTGCTGTGGGTCAGCGTGGGAACCCGTTTTGGCTTGG‑CCTTaaag                                                                                                                                                                                             >  1:1065461/1‑237 (MQ=255)                                                                 gctgg‑cA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCCAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGGTAAGGGGA‑TTGCTTTGGGTCAGCGGGGTAACCAGTTTTGGCTAGG‑CCTTAAGTTTGGTTCCGTTCGTTa                                                                                                                                                                             >  1:434553/1‑239 (MQ=255)                                                                     gcca‑caGTTTCCG‑GTAAAATATCCCCGGCGCGCCCCCGGCTTGTT‑G‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTaaa                                                                                                                                                                           <  1:11034/236‑1 (MQ=255)                                                                           gggttCCT‑GATAAATTCCACCAGGGCGCCACCTGCTTGTT‑T‑GCCCGCCACGGTCTGCTGGCGTTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTCCGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCAt                                                                                                                                                                     <  1:548005/236‑1 (MQ=255)                                                                             cTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTaaagc                                                                                                                                                                         >  1:286346/1‑231 (MQ=255)                                                                                  t‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTT‑CCGGTCTGCCACAACGCCGCTTCCAG‑CTTTTCAACGTGGTTATG‑GGG‑TTTCTGTGGGGCAGCCCTGTAAACCGTTTTTGCTATGCCCTTAAGAGTCTTTCC‑TTCCTTAAACACCCGGAAGaaa                                                                                                                                                              >  2:769039/1‑237 (MQ=255)                                                                                  t‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAC‑TTTTTTACA‑TGGTTAAGGGGG‑TTGCTGTGGGTCCGCGGGGTAACCACATTTGGCTATG‑CCTTAAGGATGATTCCGTTCGTTAAACGCCCTGACGAta                                                                                                                                                              >  2:827492/1‑238 (MQ=255)                                                                                       aaaTATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTTCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCCGCGTGGGAACCAGTTTTTGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAAACCCATGAAAATCAggg                                                                                                                                                          >  2:845742/1‑238 (MQ=255)                                                                                        aaTATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGCCTGCCAAACCGCCGCTTCCAG‑GTTTTCAAC‑TGGTTAAGGGGA‑TTGCTGTggg                                                                                                                                                                                                                                 >  1:657527/1‑168 (MQ=255)                                                                                               ccAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGGGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGt                                                                                                                                                  >  1:804745/1‑239 (MQ=255)                                                                                               ccAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTCTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGGTTGATTCCGTTCGTTAAAACCCATGAAATTCAGGTAATTACGt                                                                                                                                                  >  1:141169/1‑239 (MQ=255)                                                                                               ccAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGAGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTTTG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCCTGAAGATAAGGTAAGGCCGt                                                                                                                                                  >  1:19669/1‑239 (MQ=255)                                                                                                ccgccgcccccttttttGTT‑T‑CCCCGCCCTGGTCTGGGGGCGCTGGTGGTTGGCCTTTTTTTTGCATTTGTCCGTACGCTGCTGCAAACCAGGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGtt                                                                                                                                                 <  2:830412/223‑1 (MQ=255)                                                                                                  gcgcgcCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCCAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAAACAGTTTTGGCCATG‑CCTTAAGTATGATTCCGTTCGTTAACAGCCATGAAGATCAGGGAATGGCGtt                                                                                                                                                 >  2:944636/1‑237 (MQ=255)                                                                                                  gcgcgcCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGGCTGGTAACCAGGTTTGGCTATG‑CCTTAAGTATGATTCCTGTCGTTAAACGCCATGCAGTTAAGGTAATgcgctt                                                                                                                                                 >  1:494525/1‑232 (MQ=255)                                                                                                   cgcgcCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGCTAAGTGGA‑TTGCTTTGGGTAAGCGCGGGAACCAGTTTTTGTTATG‑ACTTACGTATGATTCGCTTCCTTAAACTCCATGAAACTAAGGAAATGaactt                                                                                                                                                 >  2:444265/1‑232 (MQ=255)                                                                                                     cgcCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCtga                                                                                                                                            >  1:830412/1‑239 (MQ=255)                                                                                                                  tt‑t‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATTAAGATCAGGGAATGACGTTCCTTAGGATCCTTCCAAt                                                                                                                               >  1:328113/1‑239 (MQ=255)                                                                                                                   ttttacgccccgctgtttgctgGGGCGGCTGGGTGGACTTTTTCTGCCTTTTGCCCTTACGCTGCTGCAAACCATGGGGTGAATTC‑CGGGTCTGCCAACCCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCaa                                                                                                                                <  2:178117/222‑1 (MQ=255)                                                                                                                     t‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGGAATGACGTTCCTGATGATCCTGCCAAtt                                                                                                                              >  1:715153/1‑238 (MQ=255)                                                                                                                       gACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAAAGCCATGAAGATCAGGGAATGACGTTCCTGATTATCCTGCCAATTg                                                                                                                             >  1:147049/1‑238 (MQ=255)                                                                                                                             cACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGGATTT‑CCCGTCTGCCAAACCGCCGCCTCCAG‑TTTTTCAAC‑TGGGTAAGTGGA‑TTGCTGTGGGTCACCGTTGTAACCAGTTTTGGCTCTT‑CCTTAAGAATGTATCCCTTCGTTAAACGCCAGAAAGCTCCGGGAAGGAGGTTCCTCTCGATACTGCCCATGCCCTTGtt                                                                                                                      >  2:579808/1‑239 (MQ=255)                                                                                                                                   ctggtgGCGCTGCTGGCTGGCCGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGAAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCg                                                                                                                 <  1:219913/238‑1 (MQ=255)                                                                                                                                    tgggggcgtgtggggctggCCGTTTCTTTGCATTTGTCCGTACGATGCTGCCAACCATGGGGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCt                                                                                                               <  1:606063/226‑1 (MQ=255)                                                                                                                                            ttgtgggccggACGTTTGCTTCAATTGGTCCGTACGTTGTTGCCAACCATGGCGGGAATTG‑CCGGTCTGCAAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGt                                                                                                        <  1:174984/233‑1 (MQ=255)                                                                                                                                            ctgctgGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGGGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCCGGTAATAACGTTCCTGATGATCCTGCCAATTGCCTTTTTAACCGCCGGCTtttt                                                                                                       >  1:351673/1‑239 (MQ=255)                                                                                                                                                gggctggACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCGGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAgg                                                                                                    <  1:618442/237‑1 (MQ=255)                                                                                                                                                  gttggcttttttCTTTCTTTTTTCCCTTCGGTTCT‑CCAAACCTGGGGGGAATTTCCCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCAc                                                                                                 <  2:916354/231‑1 (MQ=255)                                                                                                                                                  gttggacttttctttcgttttGTCGGTCCGGTTCTGCCAACATTGGGGGGATTTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGTAc                                                                                                 <  2:263860/221‑1 (MQ=255)                                                                                                                                                   ctggACGTTTTCTTGCATTTGTCCGTACGCTGGTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCAc                                                                                                 <  1:827492/238‑1 (MQ=255)                                                                                                                                                    tggctgTTTTTTTGCATTTGTCCGTACGCTGTTGCCACCCATGGCGGGAATTT‑CGGGTCGGCCAATCCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACg                                                                                                <  1:1043603/233‑1 (MQ=255)                                                                                                                                                    tggACGTTTTCTGCCATTTGCCGTTACCTGGGTTCCAACCATGGCGGGAATTT‑CGGGTCGGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACg                                                                                                <  1:916512/238‑1 (MQ=255)                                                                                                                                                                       gTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGT‑TTGCTGTGGGTTAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCTTTAAACGCCATGAAGATCAGGGAATGACGTTCCTGTTGATCCTGCCCATTGCCTTGTTAACCGCCGGCTTGTTTGGCACCG‑TGTTTGTGGGGTTTacaaa                                                                           >  1:633393/1‑239 (MQ=255)                                                                                                                                                                       gTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTACCTTGTTAACCGCTGTCTTTTTTGGGCCGCGTTTTGGGGGTGTTTaaaa                                                                            >  2:248335/1‑239 (MQ=255)                                                                                                                                                                        tCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCAAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTg                                                                                                              <  1:763328/204‑1 (MQ=255)                                                                                                                                                                            tACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTgtggtctgtcttttatac                                                                       >  1:37433/1‑225 (MQ=255)                                                                                                                                                                                 tgctgcCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAAACGCCGGCTTGTTAGGCACGC‑TGTTTGTGGGGATTAAAAAAAAACACTGt                                                                 >  1:293719/1‑239 (MQ=255)                                                                                                                                                                                    ctccAACCATGGGGGAAATTT‑CAGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTTCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTaa                                                               <  1:610002/236‑1 (MQ=255)                                                                                                                                                                                                cGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTTCCTTGTTAACAGCTGGCTTGTTAGGCACGC‑TGTTtg                                                                                        >  1:242897/1‑201 (MQ=255)                                                                                                                                                                                                            gttctccaaacccccctgttCCAT‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATTATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTccc                                       <  2:351673/221‑1 (MQ=255)                                                                                                                                                                                                                tGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGGTTAAAAAAAAAAACTGTAACGCCTGACGATTTTCCCCGTTCCCGGtg                                   >  2:980070/1‑238 (MQ=255)                                                                                                                                                                                                                 tcccaccccccgcTTCCGG‑TTTTTCAAT‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATGCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTg                                  <  2:804745/230‑1 (MQ=255)                                                                                                                                                                                                                   aaacccgccgGTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTTGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCt                                <  1:248335/235‑1 (MQ=255)                                                                                                                                                                                                                   aaacccgccgCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCt                                <  1:845742/235‑1 (MQ=255)                                                                                                                                                                                                                          ccgcTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTGCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGGTTGTGGGGATTAAAAAAAAATACCGTAACGCTTGACGATTTTCCCCGGTCCCGGGGGCTGGACggg                         >  2:816885/1‑239 (MQ=255)                                                                                                                                                                                                                          ccgcTTCCAG‑TTTTTCAAC‑TGGTTAAGAGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGTTTTTCCCCGTTCCCGTTTTGTGTACCgg                         >  1:819662/1‑239 (MQ=255)                                                                                                                                                                                                                            gtttCCAT‑TTTTTCAAT‑TTTTTAAGTGGA‑TTTCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGa                       <  2:147049/237‑1 (MQ=255)                                                                                                                                                                                                                            gtttCCAG‑TTTTTCAAC‑TGGTTAAGTGGT‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTGGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGa                       <  2:1059795/237‑1 (MQ=255)                                                                                                                                                                                                                            gtttCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAATGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGa                       <  1:294580/237‑1 (MQ=255)                                                                                                                                                                                                                            gcTTCCAT‑TTTTTCAAT‑TGGTTAAGGGGA‑TGGGTGGGGGTCAGCGTGGTAACCAGTTTTGGCTAGG‑CCTTAAGTATTATTCCGTTCGTTAACCGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCCGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGa                       <  2:293719/239‑1 (MQ=255)                                                                                                                                                                                                                             ctttcatttttttCAAC‑TGTTTAATTGGA‑TTGGTGTGGGGCAGCGTTGTAACCAGGTTTGGCTATG‑CCTTAGGTAGTATTCCGTTGGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGa                       <  2:125198/231‑1 (MQ=255)                                                                                                                                                                                                                              ttCCAT‑TTTTTCAAC‑TGGTTAATGGGA‑TTGGTGTGGGTCAGCGTGGTAACCAGTTTTGGCTTTG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGTCGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAAc                     <  1:816885/239‑1 (MQ=255)                                                                                                                                                                                                                                 cAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAACCGCCATTAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTa                  <  1:444265/239‑1 (MQ=255)                                                                                                                                                                                                                                       tttCAAC‑TTGTTAAGTGGT‑TTGCTGTGGGGCAGGGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTTTGATTCCGTTCGTTAAACGCCATGAAGAACAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTaa             <  2:855095/239‑1 (MQ=255)                                                                                                                                                                                                                                            aC‑TGGGTAAGTGGG‑TTTGTGGGGGTCGGCGTGGTAACCAGTTTTGGGTATG‑CCTTAAGTATGATTCCGTGCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTccc        <  2:633393/239‑1 (MQ=255)                                                                                                                                                                                                                                                ggTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCGTTACCGGGAACGAATTTAATTCCCCtc     >  2:155722/1‑238 (MQ=255)                                                                                                                                                                                                                                                  ttAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCa   >  1:780939/1‑239 (MQ=255)                                                                                                                                                                                                                                                   tAAGTGGA‑TTGCTGGGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAt  <  1:650761/239‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GCTGCTGGCTAAGTTATATTCAGGGGCGCTGG‑TTAGGGAATACCAAAA‑C‑GGTGAAAGGCTGGCTGG‑CA‑CAGCTTCCT‑GCTAAATATCACCAGCGCGCCACCTGCATGTT‑T‑GACCGCCACGGTCTGCTGGCGCTGCTGGCTGGACGTTTTCTTGCATTTGTCCGTACGCTGCTGCCAACCATGGCGGGAATTT‑CCGGTCTGCCAAACCGCCGCTTCCAG‑TTTTTCAAC‑TGGTTAAGTGGA‑TTGCTGTGGGTCAGCGTGGTAACCAGTTTTGGCTATG‑CCTTAAGTATGATTCCGTTCGTTAAACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGC‑TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT  >  NC_000913/3153805‑3154274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑