Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,277,498	T→G	16.4%	V55G (GTG→GGG)	yhaV →	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,498	0	T	G	16.4%	165.0 / 6.3	88	V55G (GTG→GGG)	yhaV	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (14/51);  new base G (9/6);  total (31/57)
Fisher's exact test for biased strand distribution p-value = 8.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACAAGGAAAGAAACTTGTCGCTGGCAT‑GGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCA  >  NC_000913/3277267‑3277728                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              caacaaGGAAAAAAACTTGTCGCTGGCAT‑GGACCTCAAAATTGATGATGAGGTTGGGGACGACAAATAATGGTTTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGTTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTgag                                                                                                                                                                                                                                        <  2:837743/239‑1 (MQ=255) caacaaGAAAAAAATATTGTCGTGGACTG‑GGACTTCAACATGGATGAGGAGAGTGGCGACGAAGAATAATGGTTTTTCCACAAAGGGTTAA‑TGGTGGGGTTTTTTATGCTCATCCCTGTTTTCAGGAAACCAAC‑TACGCTTTAGTTGCCGAAGTGGAAACA‑TAAAAGGG‑AAAAGATCCTAAAAATTATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTgag                                                                                                                                                                                                                                        <  2:469887/239‑1 (MQ=255) caaaaaGGAAAAAAACTTGTCTCTGGAAT‑GGACGTCAACAGTGAGGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTGGGGCGCTATAGGCTCACCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGAAA‑TTAAAGGG‑AAAAGACCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTgag                                                                                                                                                                                                                                        <  2:866713/239‑1 (MQ=255) aaacaaGGAAAGAAATTTGTCGCGGGAGA‑GGACGACAACATTGAGGAGTAGTTTGGCGACCAGAAATAAGTGATTTTCCAAAAAGGTTTAA‑TGGTTGGGCGTTATATGCCCATCCCTGTTTCAAGGAAACCTCC‑GACGCTTTAGTTGCCGAAGTGGATACA‑TAAAAGGA‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTgag                                                                                                                                                                                                                                        <  2:612668/238‑1 (MQ=255)     aaggaaataacctgtcccgtGGCAT‑GGAGTACAACATGGATGAGGAGATTGGCGACGACAAATAATGGATTTTCCAAAAAGGGTTAA‑GGGTTGGGCGCTATATGCTAAACCCTGTTATCAGGAAACCTAC‑GACGCTTAAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGc                                                                                                                                                                                                                                    <  1:726000/220‑1 (MQ=255)     aaGGAAAGAACCTTTCCGCTGGCAG‑GGACCTAAAAATTGGTGGAGAGATTGGCGACGAAGAATAATGGATTTTCCACAAAGGGTTAA‑GGGTTGGGCGATATATGCTCACCCCTGTTTCCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TAAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGc                                                                                                                                                                                                                                    <  2:1015247/239‑1 (MQ=255)       ggAAAGAAACTTGTCGCTGGCAT‑GGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGAtttgaggggat                                                                                                                                                                                                                                  >  1:660743/1‑230 (MQ=255)            gaaaCTTGTCGCTGGCAT‑GGACGTCAACATTGATGATGAGATAGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TAGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAACGGG‑AAAAGATCCTGCAAATTATCCGAGACAAGCCG‑CCCCAAACTTATTGGCGGCAGGCCAT‑CAAATGATTgagtagacatttcc                                                                                                                                                                                                                             >  1:971723/1‑231 (MQ=255)                cttgtgctgggCAT‑GGACGTAAAAATGGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGTTATATGCTCATCCCTGTTTTCAGGAACCCTAC‑GACCCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTc                                                                                                                                                                                                                         <  1:575341/230‑1 (MQ=255)                   gTCGCTGGCAT‑GGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAGACCTAC‑GAGGCTTTAGTTGCCGAAGTCG‑CACACTTAATGGG‑AAAAGATCCGGAAAATTATCGAAGAAAAGCCG‑CCACAAAGTTATTGTCGGGAGACCCA‑AAAAAGATTTAGGAGCaaaaaaagggcca                                                                                                                                                                                                                       >  2:232394/1‑226 (MQ=255)                   gTCGCTGGCAT‑GGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGGAGTACAT‑AAAATGagttaagaggacaaaaagggccaa                                                                                                                                                                                                                      >  2:53758/1‑216 (MQ=255)                    gggCTGGGATGGGACTTCAACTGTGATGGTGGGGTGGGCGCCAAAGAATAAGGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGTTATATGCTCATCCCTGTTTGAAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGAAA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAAt                                                                                                                                                                                                                      <  2:221934/237‑1 (MQ=255)                             t‑GGGCGTAAACATGGATGATGTGTTTGGCGACGACAAAAAATGGATTTTCCACAAGGGGTTAA‑TGGTTGGGCGCTAAATGCTCAGCCCTGTTTACAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TAAAAGGG‑AAAAGATCCTGAAAATTATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CAc                                                                                                                                                                                                           <  2:732899/239‑1 (MQ=255)                                     caaattttatgatgcAATTGGCGACGACGAATAATGGATTTTCCAAAAAGGGTTAA‑TGGTGGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAATTCGAGACA‑TTAAAGGG‑AAAAGTCCCTGAAAATTATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCAtt                                                                                                                                                                                                    <  1:113648/231‑1 (MQ=255)                                     cAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAACCCG‑CCACAAAGTTATTGTCGGTAGTCCAT‑AACAAGATTTAGGGGCAATACACGGTCAATCCACCAT‑CACCTGcct                                                                                                                                                                                                     >  1:952034/1‑236 (MQ=255)                                                 tgaGATTGGAGACGGCGAATAAGGGATTTTCCACAAGGGGTTAA‑TGGTTGGGCGCTATAGGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGTTTTAGTTGCCGAAGGCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCa                                                                                                                                                                                         <  1:738924/238‑1 (MQ=255)                                                  gatagtGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAg                                                                                                                                                                                       <  1:589002/234‑1 (MQ=255)                                                  gagaTTGGCGACAACAAAAAAGGGATTTACCACAAGGGGTTAA‑TGTTTGGGCGTTATATGCCCATCCCTGTTTACAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGGCGAGACA‑TTAAAGGG‑AAAAGATCCTAAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAg                                                                                                                                                                                       <  1:680850/239‑1 (MQ=255)                                                     aTTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGCAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAAAGATTCAGGGAGATATCACCGTCAAGCCACCAC‑CACCCGCCTTcctctatggtacac                                                                                                                                                                                      >  1:431240/1‑225 (MQ=255)                                                     aTTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACCAAGTTATTGGCGGGAGTCCAT‑AAAATGGTTAAGGAGGATAACAAGGTCAAACCACCATACACCgaaatacctcataggaaa                                                                                                                                                                                        >  2:309135/1‑219 (MQ=255)                                                       tGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAA‑AAAAGGCTTGAGGAGCCAATAAAGGTCAATACATCAT‑CAACGGCATTTCGCCATGGCAAGTCGtt                                                                                                                                                                                  >  1:331828/1‑239 (MQ=255)                                                            tctacgaATTATGTTTTTTCCAAAAAGGGTTAA‑GGGTTGGGCGTTATTTGCTCATCCCGTTTTTAAGGAAACCTAC‑CACGCTTTAGTTGCCGAAGTCGAGACA‑TAAAAGGG‑AAAAGATCCTGAAAATTAGCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGct                                                                                                                                                                            <  2:716838/236‑1 (MQ=255)                                                             cgacgaATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGGAGTCCCTAAAAGGGTTTGGGGGGCA‑ATACCGGGCAATCCATCAT‑CACCGGGCTTTCCCAATGGCAAATGGTT‑Aggg                                                                                                                                                                             >  1:215978/1‑236 (MQ=255)                                                                   atatggttttttCCAAAAGGGGTTAA‑TGGTGGGGGCTTATAGGCCCATCCCTGTTTCAAGGAAACCTAC‑CACGTTTTAGTTGCCGAAGGCGGGACA‑TTAAAGGG‑AAAAGCGCCGGAAAATGATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGa                                                                                                                                                                     <  2:476774/230‑1 (MQ=255)                                                                   atagtttaTTTTCCACAAGGGGTATA‑TGTTGGGGGGTTTTGGTCTCATCCGTTTTTAGAAAAACACTAC‑GGCGTTTTTGTTGCCAAAGCAGAAAAA‑TGAAAGGA‑AAAAGACCGTAAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCAGCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGa                                                                                                                                                                     <  2:384585/231‑1 (MQ=255)                                                                   ataatGGATTTTCCCAAAAGGGTTAA‑TGGTTGGGCCTTATATGCTCACCCCTTTTTTCAGGAAACCTAC‑GATGCTTTAGTTGCCAAAGTCGAGACA‑TTAAAGGG‑AAAAGTTCCTAAAAATTATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGa                                                                                                                                                                     <  1:1076625/238‑1 (MQ=255)                                                                     aatGGATTTTCCAAAAAGGGTTAA‑GGGTTGGGCGCTATATGCTCATCCTTGTTTTCAGGAACCCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCcatcat                                                                                                                                                                                                               <  1:543344/197‑1 (MQ=255)                                                                           ttttCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAAGGAATTAGGAGCCTAATAATGGCAATCCATCAT‑CACCGGCATTCCGTCATGGCCAGGCGGT‑AGGCCCTGGGGAAAAATaaa                                                                                                                                                             >  1:118317/1‑239 (MQ=255)                                                                            ataggagaaaGGGTTAA‑TGGTGGGGCGTTTTATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCGAAAGTCGAGAAA‑TTAAAGGA‑AAAAGATCCTGAAAATTATAAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGa                                                                                                                                                           <  1:661832/232‑1 (MQ=255)                                                                              tCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGACCCT‑AAAGGATTTGAAGAGAATATAATGGGCAATCCATCAA‑CACCTGCATTCCTGCATTGCCAGTCGGT‑AGGCTCT‑GGGGAAAATaaaaaac                                                                                                                                                         >  1:44050/1‑235 (MQ=255)                                                                                   aaaGGGGTAAGTGGTTGGGGACTATTGCCCCATCCCT‑TTTTGAAGAAAACTACGGGCGTTTTAGGTGCCGAAGTCCAGCCA‑TTAAGGGG‑AAAAGACCCTGAAAATTATCGGAAAAACGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑Tc                                                                                                                                                    <  2:659671/239‑1 (MQ=255)                                                                                       ggTTAA‑TGGTTGGGCGTTATATGCTCATCCCTTTTTACAGGAAACCTCC‑GACGTTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGt                                                                                                                                                                                                                          <  2:193355/168‑1 (MQ=255)                                                                                                  tGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGGGATTGAGGAGCATATCACGGTCAATCcatca                                                                                                                                                                                                                >  1:193355/1‑168 (MQ=255)                                                                                                  tGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGGAGTCCAT‑AAAGAAATTTAGGAGCAAAACACAGGCAAACCATCAT‑CACCCGCATTCCCTTCAGGCAAGGCGTT‑TGGGTCC‑GGGGAAAATAAAAACAGGTTCAC‑AGGAAAAATTTg                                                                                                                                     >  1:23267/1‑239 (MQ=255)                                                                                                   gggCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCCT‑AAAGAGATTGAGGAGCATATTACGGTTAATCCATCAT‑CACCGGGATTCCGTCATTGCAAGACTTT‑AGGGTCT‑GGGAAAAATAAAGACTGG‑GCAC‑CGGGAAAATTTTGt                                                                                                                                   >  1:105750/1‑239 (MQ=255)                                                                                                   gggCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAATGATTGAGGAGCATATAACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATTAAAACTGG‑GCAC‑GGGGAAAATTTggg                                                                                                                                   >  1:217870/1‑238 (MQ=255)                                                                                                         tataTGCTCATCCCTGTTTTCAGGAACCCTAC‑GACGTTTTAGTTGCCGAAGTCGAAACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGa                                                                                                                                                           <  1:525664/211‑1 (MQ=255)                                                                                                         tataTGCTCACCCCTGTTTTGAGGAAACCTCC‑GCCGTTTTAGTGGCGAAAGTCGAGAAA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTgg                                                                                                                              <  1:930374/238‑1 (MQ=255)                                                                                                                tcacctcttttttCAGGAAACTACC‑GCAGTTTTAGTTCCAGAAGACAAGACA‑TTAAAGGA‑AAAAGATCCAGAAAATTACAATACAAACGCCG‑CCCCAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATc                                                                                                                     <  2:707822/230‑1 (MQ=255)                                                                                                                tCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGGCCAT‑AAAATGATTGAGGGGCACATCACGGTCAATCCCTCCT‑CACCCGCATTCCGCCAAGGCAAGTCGTTAAGACCCT‑GGG‑AAAATAAAGACTGG‑GCAC‑GGGGAAAAATTGGGGTCGGT‑CGGTATc                                                                                                                     >  1:274357/1‑239 (MQ=255)                                                                                                                   tCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCCT‑AAAAAGATTGAGGAACAAAACACGGGCAATCCAACCT‑CAACCGACTTCCGCCATGGCAAGTGGTT‑AGGGGCT‑GGGGAAAATAAAAACAGG‑GCCCTGGGAAAAATTTggggtggtcagtatag                                                                                                                      >  1:897907/1‑223 (MQ=255)                                                                                                                   tCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGAGATTGAGGAGCATATCCCGGTCCATCCATCAT‑CACCGGCATTCCGTCATGGCAAGGAGGT‑AGGCTCT‑GGGTAAAATAAAGACTGG‑TCCC‑GGGGAAAATTTGTTGCTGGT‑CGttttat                                                                                                                    >  1:785717/1‑233 (MQ=255)                                                                                                                       tGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAAGGATTTAGGGGAAAATCACGGTCAAACCATCAT‑CACCGGCCTTCCGTCATGGCAAAACGGT‑AGGCTCT‑GGGAAAAAAAAAAACAGG‑GCAC‑GGGGAAAATTTGGGGCTGGG‑GGTTATCGTCcctt                                                                                                              >  1:947182/1‑239 (MQ=255)                                                                                                                        gTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCCT‑AAAAGGATTTAGGAGACTATCACGGGCAATCCCTAAT‑CACCGGCACTTCGTCATTGCAAATGGGT‑AGGCGCT‑GGGGAAAAAAAAAACTGG‑TGAC‑GGGGAAAAATTGGTGTTGGT‑CGtttttcgctcttt                                                                                                             >  2:971771/1‑228 (MQ=255)                                                                                                                              agaaaaCCTCC‑GACGCTTTAGTTGCCGAAGTCGAGAAA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGt                                                                                                       <  1:1012267/236‑1 (MQ=255)                                                                                                                                  aaCCTAC‑GACGCTTTAGTTGCCGAAGGCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTta                                                                                                     <  1:389903/237‑1 (MQ=255)                                                                                                                                         cgagcttttgggtaccGAAGTGGGGAAA‑TAAAAGGG‑AAAAGATCCTGAAAATTCTCAGAGAAACGCCG‑CCACAAAGTTAATGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGaaa                                                                                            <  2:331828/225‑1 (MQ=255)                                                                                                                                               tttAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCCT‑AAAGTGATTTTGGGGCAAATCACGGGCAATCCATCAT‑TACCCGCCTTCCGTCATGGCACGTCGTT‑GGGGTCT‑GGGGAAAAAAACAAATAG‑GCAC‑GGGGAAAATTTTGGGCTGGTGGGTTAT‑GTCTCTTCTTTTTTTAT‑AGAGaaaaaagaa                                                                                      >  2:1005752/1‑235 (MQ=255)                                                                                                                                               tttAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCCT‑AAAAGGGTTGGGGGGCATATACCGGTCCATCCATCAT‑CACCGGCAATCCCTCATGGCAAGACGTT‑AGACTCT‑GGGGAAAATAAAAAATGG‑ATCC‑GGGGAAAATTTTGTGGTGGG‑CGTTATTTTCTCTTTTTTTGCTATGAGTaaaaaaaaa                                                                                       >  2:370956/1‑236 (MQ=255)                                                                                                                                               tttAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGAGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCCAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑GCAC‑GGGGAAAATTTGGGGCTGGT‑CGTTATCGTCCCTTTTTTCGTTAT‑AGTTaaaaaaaaa                                                                                      >  1:228469/1‑235 (MQ=255)                                                                                                                                               tttAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAATCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATTGCAAGACGGT‑AGGGTCT‑GGGGAAAATAAAGACTTG‑GCAC‑GGGTGAAATTTTGGGCTGGT‑TGGTTTCGTGCCttttttttgtttaatggaaaaaaga                                                                                       >  1:320236/1‑220 (MQ=255)                                                                                                                                                 ttgtttgacaaggtcgggaa‑TTAAAGGGAAAAAGATCGGAAAAATTATCAAAAAAAAGCCG‑CCCCAAAGTTTTTGGCGGGAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CCCCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGaa                                                                                     <  2:657312/220‑1 (MQ=255)                                                                                                                                                 tAGTTGCAGAAGTCGAGACA‑TAAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGaaa                                                                                    <  1:309135/239‑1 (MQ=255)                                                                                                                                                    gtGCCAAAGCCGAAACT‑GTAAAGGG‑AAAAGAACCTAAAAATTACCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAA‑TTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGtca                                                                                <  1:289748/238‑1 (MQ=255)                                                                                                                                                     tgccaaggctgaaaCA‑TTAAAGGA‑AAAAGCTCCTGAAAATAAAAAGAGAAAACCCG‑CCACAAAGTTATTGGCGGTAGGCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGtca                                                                                <  2:1008622/226‑1 (MQ=255)                                                                                                                                                      gCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAATGATTGAGGAGCATATCACGGTCAAACCATCAT‑CACCGGCATTCCGTCATGGCAAAGCGGT‑AGGCTCT‑GGGAAAAATAAAGACTTG‑TCCC‑GGGGAAAATTTTGGGCTGGT‑GGTGTTCGTCTCTTCTTTTTTTTT‑AGGGAAAAAAAGaaaagcat                                                                               >  1:1074119/1‑234 (MQ=255)                                                                                                                                                           aagtggaaaatttAAAGGG‑AAAAGTCTCTGAAATTTATCAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCAtt                                                                           <  2:215978/228‑1 (MQ=255)                                                                                                                                                              cgggaaa‑TTAAAGGA‑AAAAGGTCCAAAAAATTAAAGAAAAAAAGCCG‑CCACAAAGTTATTGGGGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTgg                                                                       <  2:947182/233‑1 (MQ=255)                                                                                                                                                                 aata‑TTAAAGGG‑AAAATATCCTAAAAATTTTAAGAGAAAGGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGgga                                                                     <  2:32470/235‑1 (MQ=255)                                                                                                                                                                        aaaGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGGCCAT‑AAAGTGATTGAGGAGCATATCAAGGTCAATCCATCAT‑CACCAGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑GCAC‑GGGGAAAATTTGGTGGTGGT‑GGTTATTGGCTCTTTTTTTGTTAT‑AGGGAAAAAAAGAAAATAATAATTATggggaggggga                                                              >  2:298402/1‑231 (MQ=255)                                                                                                                                                                          aGGT‑AAAAGTACCTGAAAATTCTCAAAAAAATGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                            <  1:232394/239‑1 (MQ=255)                                                                                                                                                                          aGGA‑AAAATATCCAAAAAATTAACAGAAAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                            <  2:1074119/239‑1 (MQ=255)                                                                                                                                                                          aGGA‑AAAAGATCCTGAAAATTATAAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                            <  1:57618/239‑1 (MQ=255)                                                                                                                                                                          aGGA‑AAAAGATCCAAAAAATTAACAGAAAAAAGCCG‑CCACAAAGTTTATGGCGGTTGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                            <  2:118317/239‑1 (MQ=255)                                                                                                                                                                             gaaaagtatacggAAAATATTCAGGAAAAGGCCG‑TCACAAAGTTATTGGCCGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCAGTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTATCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGTAc                                                            <  1:459937/225‑1 (MQ=255)                                                                                                                                                                              aaaaagcatccaaaaataaaaagaaAAAAGCCC‑CCCCAAAGGTATTGGCGGTAGTCCAT‑AAAGGGATTGAGGAGCATATCACGGTCAAGCCATCAG‑CACCGGCATCCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAAGAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTGCTTGCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGa                                                          <  2:539593/217‑1 (MQ=255)                                                                                                                                                                                    atctaaaaatTTTTCAGAGAAACGCCC‑CCACAAAGTTATTGGCGGTAGTCAAA‑AAAGTTATGGGGGAGCATCTTACGGTCAAGCCATCAA‑CGCCGGCATTCCGTCATGGCAAGTCGTT‑AGGCGCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAac                                                   <  2:815187/233‑1 (MQ=255)                                                                                                                                                                                    aTCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCAAATCACGGGCAATCCATCAT‑CACCGGGATTCCGTCATGGGAAGGCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGGGCTGGG‑CGTTATCGGCTCTTCTTTCGTTAT‑ATTGAAAAAGAGAAAATCATCATTCTTGGATGGATCAACGATGaaaaa                                                   >  1:114907/1‑238 (MQ=255)                                                                                                                                                                                       cTGAAAATTAACAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGGCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACAct                                                <  1:720264/239‑1 (MQ=255)                                                                                                                                                                                          aaaaTCAGCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATACCACGGTCAAGCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTg                                             <  1:53758/239‑1 (MQ=255)                                                                                                                                                                                                attggaaaaaaGCCG‑CCTCAAAGTTATGGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCCCGGTACATCCAGCAT‑CACCGGCACTCCGTCATGGCAAGGCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTGCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCAcc                                       <  2:752446/232‑1 (MQ=255)                                                                                                                                                                                                   agaaAAAAGCCG‑CCAAAAAGTTATTTGCGGTATTCCAT‑AAATGGATTGAAGCGCATATCCCGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGGCTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                    <  1:425076/239‑1 (MQ=255)                                                                                                                                                                                                      aaaaaCCCC‑CCAAAAAGTTATTGGCGGGAGTCCAT‑AAAGTGATTTAGGAGCATAACCCGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                    <  2:897907/235‑1 (MQ=255)                                                                                                                                                                                                       aaaaGCCCCCCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                    <  2:1044102/236‑1 (MQ=255)                                                                                                                                                                                                         aaGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAATGATTGAGGGGCATATCACGGTCAATCCATCAT‑CACCGGCCTTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAAAAAAAACTGG‑TCAC‑GGGGAAAATTTGGTGCTGGG‑CGGTATCGTCTCTTCTTTCGTTAT‑AGGGAAAAAGAGAAAATCATCATTCTGGGGTGGATGAAACATGAAAAAACCTTGCGCCCCTCCGGTaaa                              >  1:1003045/1‑239 (MQ=255)                                                                                                                                                                                                              cggccaaaaGGTTTTGCCGGGTGTCCAT‑AAAGGTAATGAGGGGCTAATCACGGGCAAGCCCTCAT‑TCACGGCATTCCGTCATGGGAAGTCCTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑CGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaa                            <  1:425868/231‑1 (MQ=255)                                                                                                                                                                                                                gcacaAAGTTTTTGGGGGTAGTCCAA‑AAAGTTATTTGGGAGCATTGCACGGTCAAGCCATCAT‑CACCGGCATTCCGTCATGGCAAGGCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                        <  2:592831/238‑1 (MQ=255)                                                                                                                                                                                                                cctcaAATTTTTTGGCGGTATTCCAA‑AAAGTACTTGAGGAACATTACAGGGTACATCCAACAT‑CAACGGCATACCGGCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                        <  2:705727/236‑1 (MQ=255)                                                                                                                                                                                                                ccccaAAGTTATTGGCGGTAGTCCAT‑AAAGTGAATGAGGGGCATATCACGGTCAATCCATCAG‑CACCGGCATTCCGGCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                        <  2:873103/236‑1 (MQ=255)                                                                                                                                                                                                                ccACAAAGTTATTGGCGGTAGTGCAT‑AAAAGAGTTTAGGGGGATATCACGGTCAATCCCTCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGGTCT‑GGGGAAAAAAAAGAATGG‑TCAC‑GGGGAAAATTTTGTGCTGGT‑CGTTATCGTCTCTTCTTTTGTTAT‑AGTGAAAAAAAGAAAAGAATCATTTTGTGGTGGATGAACGATAGAAAAACTCTTGGCCCCTAAGGGgaaaaaaaa                        >  2:1081596/1‑237 (MQ=255)                                                                                                                                                                                                                ccACAAAGTTATTGGCGGTAGTCCAT‑AAAATGATTGAGGGGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCCAATCGTT‑AGGCTCT‑GGGGAAAATAAAAACTGG‑GCAC‑CGGGAAAAATTGGTGCTGGG‑AGTTTACGTTCCTTTTTTTGTTAT‑AGTGAAAAAGAGAAAATCATCATTTTGGGATGGGTGAACGTTGAAAACAATCTGCGGACCCACGGTaaaaaaaac                        >  1:222981/1‑237 (MQ=255)                                                                                                                                                                                                                 cacaAAGTTAATGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCAACAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                        <  1:38279/238‑1 (MQ=255)                                                                                                                                                                                                                          tttGGCGGTTGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATGTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata              <  2:429885/238‑1 (MQ=255)                                                                                                                                                                                                                          aTTGGCGTTAGGCCAT‑AAAGGGATTGAGGGGCATATACCGGTCAATCCATCAT‑CACCGGACTTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGCCGGG‑TCAC‑GGGTAAAAGTGGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata              <  2:249127/239‑1 (MQ=255)                                                                                                                                                                                                                          aTTGGCGGTAGTCCAT‑AAAGGGATTGAGGAGCATTGCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata              <  1:319912/239‑1 (MQ=255)                                                                                                                                                                                                                           ttGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAAATTGGGGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAAAGAAAGTCATCATTTTGGGATGGATGAACGATGAAAAAACTCTTCGCACCTACGGTAAAAAAACAAATGCCtcta              >  1:109962/1‑238 (MQ=255)                                                                                                                                                                                                                                ggTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTa         <  1:370956/238‑1 (MQ=255)                                                                                                                                                                                                                                      ccAT‑AAAGGGATGGAGGAGGATTTCACGGGTAAGCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACGGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCa  <  2:952034/239‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              CAACAAGGAAAGAAACTTGTCGCTGGCAT‑GGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAA‑TGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC‑GACGCTTTAGTTGCCGAAGTCGAGACA‑TTAAAGGG‑AAAAGATCCTGAAAATTATCAGAGAAAAGCCG‑CCACAAAGTTATTGGCGGTAGTCCAT‑AAAGTGATTGAGGAGCATATCACGGTCAATCCATCAT‑CACCGGCATTCCGTCATGGCAAGTCGTT‑AGGCTCT‑GGGAAAAATAAAGACTGG‑TCAC‑GGGTAAAATTTGGTGCTGGT‑CGTTATCGTCTCTTCTTTCGTTAT‑AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCA  >  NC_000913/3277267‑3277728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑