BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,565,286	T→G	10.9%	H432P (CAC→CCC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,286	0	T	G	10.9%	274.7 / 3.1	129	H432P (CAC→CCC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base T (62/46); new base G (3/16); total (67/62)
Fisher's exact test for biased strand distribution p-value = 9.34e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

TTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGAGAAG  >  NC_000913/3565050‑3565522

   ggAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CGGATTAGAGTGCAGTTCGTATACACCGTTAAATTTGTGGccc                                                                                                                                                                                                                                             >  2:361432/1‑237 (MQ=255)

        tCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAACCAACGATTGCACCAC‑CCGATTAGAGTGCAGTTCCGATACTCCGTTAACTTTTTGGCCca‑cca                                                                                                                                                                                                                                        >  1:861855/1‑236 (MQ=255)

                ttgttgCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACGGTTAACTTTTTGGCTCA‑C‑AA‑CAACcccc                                                                                                                                                                                                                              >  1:823588/1‑238 (MQ=255)

                   ttggcgcattttAAGCAGGCTAAGGTGGGGGGCCCGGTTCAGGCCCAGGTGTTCGGCCGGCACGGCGGAAAGGGATGGGTTCGGTACCGCCGGCCAGCGAC‑GGGGCGTCACACCGTTGGTTGCGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAGCTTTGTGGCTCA‑C‑AA‑CAACCgccagcc                                                                                                                                                                                                                          <  2:355240/229‑1 (MQ=255)

                   ttgCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGCCGGCGAACAACGATTGCACCAT‑CAGATTAGATGTCAGTTCCGATACACCGGTAACTTTTTGGCTCC‑C‑AA‑CAACCgccaccc                                                                                                                                                                                                                          >  2:958101/1‑239 (MQ=255)

                        gCTCATAAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGGGACGTTGGTGAACCGACCCCGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATAAACCGTTACATTTGCGGCTCC‑C‑AA‑CAACAcccagcccgacc                                                                                                                                                                                                                     >  2:639215/1‑234 (MQ=255)

                            aTTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AC‑CAACCGCCAGCCAGGCCATa                                                                                                                                                                                                                  >  1:175897/1‑238 (MQ=255)

                            aTTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATa                                                                                                                                                                                                                  >  1:95977/1‑238 (MQ=255)

                              tAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGGGACGTTGGTGAAACGACCCGGGCAGATTTTCGCAAAGTCGGAAAACAACGATTGCACCAC‑CAGATTGGAGGGCATTTCCGATACCCCGTAAACTTTATGGCCCA‑C‑AA‑AAACACCCAACCAGGcaaaaaca                                                                                                                                                                                                               >  2:381096/1‑232 (MQ=255)

                                     gCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCCTACGCACac‑gac                                                                                                                                                                                                       >  1:38046/1‑239 (MQ=255)

                                     gCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGCTGGGGACGTTGGCGAAACGACCCGGGAAGATTTTCGCAACGACGGCAAAAAACGATTGAACCAC‑CAGATTAAAGGGCAGCTCCGAGACACCCTTACCATTTTGGCCCC‑C‑AC‑CAACCGCCCCCCAAGCCCTAccccacccac                                                                                                                                                                                                        >  2:903594/1‑230 (MQ=255)

                                      caaggggcggtgggcctttgccGGCCCAGGGGTCGGGCCGGCCCGGTTGAAAGGGGTGGTGTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACCCCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCATTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACac‑gac                                                                                                                                                                                                       <  2:1021250/217‑1 (MQ=255)

                                               gTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTTGGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCAAAC‑GACGCCCGTTGGa                                                                                                                                                                                             >  2:471671/1‑239 (MQ=255)

                                               gTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCC‑C‑AA‑CAACCGCCAGCCAGGCCATACGAACAC‑GACGCCCGTTGGa                                                                                                                                                                                             >  1:464655/1‑239 (MQ=255)

                                               gTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGa                                                                                                                                                                                             >  1:1011306/1‑239 (MQ=255)

                                               gTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑A‑AA‑CAACCGCCAGCCAGGCCATACGCACCA‑GACGACCGTGGGa                                                                                                                                                                                             >  1:296113/1‑239 (MQ=255)

                                               gTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGGGAAACGACCCGGGAAGCTTTTCGCAAAGTCGTATAACAACAATTGCACCAT‑AAGATTATGTTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑A‑AA‑CAACCCCCAGCACGGCCATACCCCAAC‑GCCAACCGTTGGa                                                                                                                                                                                             >  2:228535/1‑239 (MQ=255)

                                               gTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGACGGGTTCGCTACCGCCAGCCAGCAAC‑GCGGCGTCACACCGTTGGTGACGTTGGCGAACCGACCCGGGAAGATTTTCGCAAAGTCGGCAACCAACGATTGCACCAC‑CAGCTTAGAGTGCAGCTCCGCTACACCACTAACTTTGAGGCTCA‑C‑AA‑CAACCCCCCGCCCGTCCATACCCacaccagacaagccgga                                                                                                                                                                                              >  2:427879/1‑226 (MQ=255)

                                               gTGCGCCAGTTACGGCCCAGGTGGTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑ACGGCGTCACACCGTTGGTTACGTTGGTGAAACGACCCGGGCAGATTTTCGCAAAGTCGGCAAACAAGGATTGAACCAT‑CGGATTCAAGCGACGTTCCGATACACCGTTAACATTGTGCTCCA‑C‑AA‑CAACCGCCGGCCCGGCCCTACGACCAC‑CCAGACCATTGGa                                                                                                                                                                                             >  2:952118/1‑239 (MQ=255)

                                                  ggcCGGTTCCCGCCCAGGTGTGCGCCCAGCGCGGGTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GGGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGAtt                                                                                                                                                                                           <  1:1003088/237‑1 (MQ=255)

                                                  cgccgtttccGGCCCGGGTGTGCTGCCAGCGCGGGTGAAGGGGGTGGGTTCGGTGCCCCCAGCCAGCGGC‑GGGGGTTCCCCCCGTTGGGGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGAtt                                                                                                                                                                                           <  2:10162/229‑1 (MQ=255)

                                                          aCGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGa                                                                                                                                                                                  >  1:21018/1‑239 (MQ=255)

                                                              cccAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAG‑CAGATTAGAGTGCAGTTCCGATACACCGGTAACTTTGGGGCTCC‑C‑AC‑CAACCGCCAGCCCGGCGATACCAACAC‑GCCGACCGTGGGaacaataaatgaaggc                                                                                                                                                                              >  2:378270/1‑224 (MQ=255)

                                                                cgggttttccgccgccccGGCTGAAAGCGATGGGTTCCCTACCGCCAGCAAGGGAC‑CGGGGGTCCCCCCGTGGGTGACGTGGGTGAAACGACCCGGGAAGATTTTCGAAAAGTCGGCAAAAAACGATTGCCCCAT‑CAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACg                                                                                                                                                                            <  2:793661/222‑1 (MQ=255)

                                                                cAGGTGTTGGTCAAGCACGGCTAAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GGGGCGTCACACCGGTGGTGGCGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACg                                                                                                                                                                            <  1:664521/239‑1 (MQ=255)

                                                                 aGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AC‑AAACCGCCAGCCAGGCCATACGCACCC‑GACTACCGTTGGATTAATCAATGATCGACg                                                                                                                                                                            >  1:523969/1‑238 (MQ=255)

                                                                 aGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCAATACGCAAAC‑GCCGACCGTTGGATCCATAAATGATCGCCg                                                                                                                                                                            >  1:311735/1‑238 (MQ=255)

                                                                  ggtttgcgccccgCAGGGCTGAAAGGGGTGTGTTCGCGTCCGCCAGCCGGCGAC‑GGGGCGGCCCACCGTTGGGGACGTTGGTGAAACGACCCGGAAAGATTTGCGCAAAGGCGGAAACAAACGATTGCACCAC‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGcc                                                                                                                                                                          <  2:523969/227‑1 (MQ=255)

                                                                  ggTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GCCGACCGTTGGATTCATCACTGATCGACGc                                                                                                                                                                           >  1:625463/1‑238 (MQ=255)

                                                                  ggTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACCCCAAC‑GACGCACGTTGGATTCATCAATGATCGACGc                                                                                                                                                                           >  1:1079537/1‑238 (MQ=255)

                                                                  ggTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAACCAGGCCATACGCACAC‑GACGACCGTTGGATTCACCAATGACCGACGc                                                                                                                                                                           >  1:203567/1‑238 (MQ=255)

                                                                        cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑AACGACCGTTGGATTCATCAATGATCGACGCCCGTccc                                                                                                                                                                    >  1:722448/1‑239 (MQ=255)

                                                                          tccgagctgggtaaaaggggggggTTGGCTTCCGCCAGCCAGGGAT‑GGGGCTTCAGACGGTTGGTGACGGTGGTAAAACTCCCGGGGAAGAGTTTCGAAAAGTCGGCAACAAACGTTTGCCCCAA‑CAGTTTAGAGTGCAGTGCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                                                                                                                                  <  2:237076/218‑1 (MQ=255)

                                                                          gccggCACGGTTGAAAGCATGGGGTTCCTCACCGCCCGCCGGCGGC‑GGGGCGGCCCCCCGGTGGGGCCGTTGGGGAAACGACCCGGGAAGATTTTCGAAAGTTCGGCAAACAACGATTTCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTACCTTTGGGGCTCC‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                                                                                                                                                                  <  2:804114/235‑1 (MQ=255)

                                                                             aGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCAGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGCCCATACCACCAC‑GACGACCGTTGGATTCAACAACAATCGCCGCCCGGCCcacaca                                                                                                                                                               >  2:355093/1‑235 (MQ=255)

                                                                                cgggttGAAAGCGATGGGTCCCGTACCGCCAGGCAGCGAC‑GCGGCGTCACACCGTTGGTGACGGTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGAt                                                                                                                                                             <  1:744331/233‑1 (MQ=255)

                                                                                    cggAAAGGGGTGGGTTCGCTCCCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGt                                                                                                                                                         <  1:830773/236‑1 (MQ=255)

                                                                                    cTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGCAGATTTTCGCAAAGCCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGCTCGACGCCCGTCCCATCAGATCGGTa                                                                                                                                                        >  1:114506/1‑239 (MQ=255)

                                                                                      taagtgcgggggTCCGCCACCCCCCGCGAGGGAC‑GCGGCGTCACACCGTTGGGGCAGGGGGGGAAACGACCCGGGAAGGTGTTCGCAAAGTCGGAAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTAt                                                                                                                                                       <  2:295056/229‑1 (MQ=255)

                                                                                       tgacggttgtgTTCGTTACCGCCAGGGAGGTGCGGCGGCGCGACACCGTTGTGGACTGTGGGGAAACGAGCCGGGAAGCTTTTCGGAAAGTGGGAAAACAACGTGTTCACGAT‑CAGATTAGAGTGCAGTTCCGATACCCCGGTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTAt                                                                                                                                                       <  2:203567/228‑1 (MQ=255)

                                                                                            gATGGGTTCGCTACCGCCAGCCAGCGAC‑TCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAACCAACGATTGCCCCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTc                                                                                                                                                  <  1:671639/237‑1 (MQ=255)

                                                                                                ggTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCAGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGCCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATa                                                                                                                                             <  1:355093/238‑1 (MQ=255)

                                                                                                    cccgcccGCCAGCCCGCGAC‑GGGGCGTCCCACCGTTGGTGACGGTGGGGAAACGACCCGGGAAGGTTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGGGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCATCAGATCGGTATCGTTCGGATACTGt                                                                                                                                         <  1:903594/233‑1 (MQ=255)

                                                                                                     gCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGa                                                                                                                                                                                             >  1:657446/1‑185 (MQ=255)

                                                                                                          cGCCAGCCTGGGCC‑GCGGCGTCACACCGTTGGGTACGTTGGTGAAACGACCCGGGAAGTTTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGGGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                                                                                                                                  <  1:560662/239‑1 (MQ=255)

                                                                                                          cGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGAATCGATCGGAAACTTTTCCTGCa                                                                                                                                  >  2:56479/1‑239 (MQ=255)

                                                                                                           cccgcgcccgccaccggcgctcccacGGTTGGTGCCGTGGGTGAAACGCCCCGGGAAGTTTTTCCAAAATGCGGCAAACAAAGATTGCCCCAT‑CTGATTAGGCGGCAGTTCCGATCCCCCGTTAACTTTGGGGCTCA‑A‑CA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGACCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCa                                                                                                                                  <  2:1073812/216‑1 (MQ=255)

                                                                                                                 caggGAG‑GCGGCGTCACACCGTTGGTGACGTTGTTGAAACGACCCGGGAAGATTTTCGCAAAGGCGGCAAACAACGATTGCACCAT‑CAAATTAGAGTGCAGTTCCGATACCCCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGtttt                                                                                                                           <  1:176658/239‑1 (MQ=255)

                                                                                                                 caggGAC‑GCGGCCTCACACCGGTGGTGACGTTGGTAAAACGACCCGGGAAGATTTGCGCAAAGGCGGCAAACAACGATTGCACCAT‑CAGATAAGAGGGCAGTTCCGATACACCGTTAACTTGGGGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGtttt                                                                                                                           <  2:175897/239‑1 (MQ=255)

                                                                                                                       c‑gcgGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAAACGCCAGCCAGGCCATACGCACAC‑GACCACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGATACGTTCGGATCCTGTTCCCGCAAGGATTTCAGGaa                                                                                                                     >  2:776965/1‑239 (MQ=255)

                                                                                                                                 acacCGTTGGGTGGGTTGGTTAAAGGGACCGGGAAGATTTTCGCAAACGGGGCAAGCAACGATTGCCCCATACAG‑TGAGGGTGCGTTTCCGGTACCCCGTTACATTTGGGGCGCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGtt                                                                                                            <  2:861855/239‑1 (MQ=255)

                                                                                                                                    cctgtggtgacgtgggggaAACGACCCGGGAAGATTTCCCGAAGGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGAt                                                                                                         <  1:407346/235‑1 (MQ=255)

                                                                                                                                        tggtgacgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGACCGGAATCGTTCGGATCCTGTTCCTGCACGGTTTTCAGGAAAAAGACGTTGATTTCa                                                                                                     >  1:858810/1‑239 (MQ=255)

                                                                                                                                         ggttggtttgggaaaaCGCCCCGGGAAGTTTTTCCAAAAGTGGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACCCCGTTAACTTGGTGGCTCA‑A‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                                    <  2:300292/226‑1 (MQ=255)

                                                                                                                                         ggtgacgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCCCCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                                    <  1:776965/239‑1 (MQ=255)

                                                                                                                                         ggtgacgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGCCCCACCAGAACGGAATCGTTCGGAAACTGTTCCTGCAAGGGTTTCAGGAAAAAGTCGTTGATTTaaa                                                                                                    >  2:120589/1‑239 (MQ=255)

                                                                                                                                         gggcagtgtgggaaaaagccccGGGAAAATTTTCGAAAAGCCGGCAAAAAACGATTGCCACAA‑CAGATTAGGGGCCAGTGCCGATACACCGTTAACTTGGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCCTACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCaa                                                                                                    <  2:114506/220‑1 (MQ=255)

                                                                                                                                            ggcttgtgtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGAAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCTTTAACTTGTGGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAga                                                                                                 <  2:255919/232‑1 (MQ=255)

                                                                                                                                            aacgttggtgaAACGCCCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAg                                                                                                  <  1:220238/237‑1 (MQ=255)

                                                                                                                                             acgttgggtaAACGACCCGGGAAGATTTTCGAAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTCCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAga                                                                                                 <  1:471671/238‑1 (MQ=255)

                                                                                                                                                 tggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAtatga                                                                                              >  1:435982/1‑237 (MQ=255)

                                                                                                                                                  ggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGAATCGTCCGGGTACTGTTCCTGCAAGGTTTTCTGGAAATAGTCGTTGATTTCAAGgatga                                                                                              >  1:278825/1‑236 (MQ=255)

                                                                                                                                                    tgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGCTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATAATCtcc                                                                                         >  2:418780/1‑237 (MQ=255)

                                                                                                                                                             ccGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGGTAcgc                                                                                >  1:805347/1‑239 (MQ=255)

                                                                                                                                                             ccGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTCCAAGGTTTTAAGGAAAACGCGGTTGATTTAAAAAATAAACTGCAGGGGAcag                                                                                >  2:883205/1‑237 (MQ=255)

                                                                                                                                                                  aaGATTTTCGCAAAGTCGGAAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATAACCCGTTACCTTTGGGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCa                                                                            <  1:135306/238‑1 (MQ=255)

                                                                                                                                                                         tcccAAGGTCGGAAAACAACGATTCCACCAT‑AAATTTAGGGGCCAGTTCCGATACTCCGTTAACTTTGTGGCTCA‑A‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTa                                                                    <  2:805347/236‑1 (MQ=255)

                                                                                                                                                                            caaacgccgCAAACCACGTTTTCCCCAC‑CAGAGTAGAGTGCACTCCCGATCCGCCGTAAACTTTGT‑GCCCA‑CAAA‑CAGCCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                                 <  2:485850/231‑1 (MQ=255)

                                                                                                                                                                            aaaaGTCGGAAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTCCCGATCCCCCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                                 <  1:32699/238‑1 (MQ=255)

                                                                                                                                                                                gTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAAATGATCTCCAGGGGACCGGGCAGAATTTAACCCAgaa                                                             >  2:189528/1‑237 (MQ=255)

                                                                                                                                                                                            cactttcccccctcggtctggagTGCAGTTCCGACACCCCTTTAACTTTGTGGATCA‑A‑AC‑CAACCCCCAGCCAGGCCATTCGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGc                                                  <  2:554943/219‑1 (MQ=255)

                                                                                                                                                                                                    ccAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGTTACTGTTCATGCAAGGTTTTCAGGAAATGTTCGTGGATTTCAAGAAGGATCTGCAGG‑GGCACGGCCGAAATTTAACCAgacaaaaaaaacggccggggtacc                                        >  2:23084/1‑216 (MQ=255)

                                                                                                                                                                                                    ccAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCACCAAATAAACCGGCCAGGTTTcc                                         >  2:560720/1‑239 (MQ=255)

                                                                                                                                                                                                    ccAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAAAATTTTACCCAGCATATAAACCGGCAAGGTTTcc                                         >  2:886427/1‑239 (MQ=255)

                                                                                                                                                                                                       t‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGCCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATTAACCGGCCAGGGTTCCac                                       >  1:619077/1‑237 (MQ=255)

                                                                                                                                                                                                           gATTAAGGTGCGGTTCCGAACCACCGTTAACTTTGTGGCTAC‑A‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                                   <  1:231901/239‑1 (MQ=255)

                                                                                                                                                                                                            aTTGGAGTGCCGTTCCGATCCCCCGTTACCTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GCCGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCAGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCt                                  <  2:625463/239‑1 (MQ=255)

                                                                                                                                                                                                             ttAGGGTCCTGTTCCGATAC‑CCGTTTACTGTGGGGCCTA‑C‑AACCACCCGCCAGCCAGGCCATACGCACCC‑GACCACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGcc                                   <  2:38046/237‑1 (MQ=255)

                                                                                                                                                                                                                   tGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCAAGGTTTCCAGCGCCTCGCtca                            >  1:1003345/1‑238 (MQ=255)

                                                                                                                                                                                                                   tGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCAAATCAACCCCCCAGGGTTCCAGGGCCCCGCtcac                           >  2:914424/1‑238 (MQ=255)

                                                                                                                                                                                                                    gcgtttCCGATCACCCTTTAACTTTGGGCCCCA‑A‑AC‑CACCCGCCAGCCAGGCCATTCGCACCC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtcat                           <  2:722448/234‑1 (MQ=255)

                                                                                                                                                                                                                    gccgTTCCGCAACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGGCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCtca                            <  1:298481/234‑1 (MQ=255)

                                                                                                                                                                                                                    gCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTAccc                                                                 <  1:467600/200‑1 (MQ=255)

                                                                                                                                                                                                                     cAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTTCAGGTGa                                                                                   >  2:908381/1‑181 (MQ=255)

                                                                                                                                                                                                                     cAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGa                                                                                   <  1:908381/181‑1 (MQ=255)

                                                                                                                                                                                                                     cAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCACCCGGCCAGGATTCCAGCGCCTCGCtcatcc                         >  1:938595/1‑238 (MQ=255)

                                                                                                                                                                                                                     cAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAACGCCTCGCtcagca                         >  1:402799/1‑239 (MQ=255)

                                                                                                                                                                                                                      aGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCCCGCtcatca                         >  1:435136/1‑238 (MQ=255)

                                                                                                                                                                                                                         tcctaatccccttttaCTTTAGGGCCCA‑A‑AA‑CACCCGCCGGCCAGGGCAATCGCACACGGACG‑CCGTTGGAATCAACAATGAACGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTGCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCgt                     <  2:85066/224‑1 (MQ=255)

                                                                                                                                                                                                                         gccgagcccccGTTAACTGTGTGGCTCA‑C‑AA‑CATCCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGAGCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCgt                     <  1:639215/230‑1 (MQ=255)

                                                                                                                                                                                                                             aTACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGGGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCCGGTTTCCACCGCCCCCCCCACCCGCGTgggg                 >  2:688192/1‑239 (MQ=255)

                                                                                                                                                                                                                             aTACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGAATCGTTCGGATCCTGTTCCTGCATGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGACCAGGTTTCCAGCGCCTCGCTCCTCAGCGTGTgg                 >  1:399113/1‑239 (MQ=255)

                                                                                                                                                                                                                              ttcacCTTTAACTTTGGGGCTCA‑C‑AA‑CAACCGGCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                <  1:189528/237‑1 (MQ=255)

                                                                                                                                                                                                                              tACCCCGTAAACTTGGTGGCCCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGGTCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                <  1:364267/239‑1 (MQ=255)

                                                                                                                                                                                                                              tACACCTGTAACTTGGTGACCCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGGTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGt                <  1:244424/239‑1 (MQ=255)

                                                                                                                                                                                                                                  ccGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAgt            >  1:1006871/1‑239 (MQ=255)

                                                                                                                                                                                                                                   ctttacttttGGGGTTCA‑C‑AA‑CACCCCCCAGCCAGGCCCACCGCACCC‑GGCGGCCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAgtg           <  2:464655/232‑1 (MQ=255)

                                                                                                                                                                                                                                   cGTTAACTTTGGGGCACA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAgtg           <  1:56479/239‑1 (MQ=255)

                                                                                                                                                                                                                                         acttGTGGCCCATC‑AA‑AAGCCACCAGCCAGGCCCTACGCACAC‑GTCGCACGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGga      <  1:378270/237‑1 (MQ=255)

                                                                                                                                                                                                                                             gtgGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGGTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGggaag  >  1:559315/1‑238 (MQ=255)

TTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAC‑GCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCAT‑CAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCA‑C‑AA‑CAACCGCCAGCCAGGCCATACGCACAC‑GACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTTTCAGGAAATAGTCGTTGATTTCAAAGATGATCTGCAGGTGACGCGGCAGAATTTTACCCAGCATATCAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGAGAAG  >  NC_000913/3565050‑3565522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`