Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	365,783	T→C	18.1%	S175G (AGC→GGC)	lacZ ←	beta‑D‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,783	0	T	C	18.1%	192.9 / 2.4	97	S175G (AGC→GGC)	lacZ	beta‑D‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (27/48);  new base C (18/4);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 1.90e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATGGGCGCATCGT  >  NC_000913/365548‑366019                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  tCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCCCATATCCTGATCTTCCAGATAACTGCCGTCACTCCCGCGCAGCCCACTCACCCCGCGCCGGTTTTCTCCGGGGCGGAAAAAACCG‑CCCAg                                                                                                                                                                                                                                                >  2:1065063/1‑239 (MQ=255) tCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATACCCGCGAGGCGGTTTTCCCCCGGGCGTAAAAATGCG‑CTccg                                                                                                                                                                                                                                                >  1:588134/1‑237 (MQ=255)   ccGCAACGCGCCGAACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGt                                                                                                                                                                                                                                              <  1:462492/238‑1 (MQ=255)             gcccacaTCTGAACTTCCAGCTCCAGGACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGa                                                                                                                                                                                                                                    <  1:6085/236‑1 (MQ=255)             ccgcccttttGAACTTCAGCCTCCGGTACAGCGCGGCGAAAATCATAATTAAAGGGAGTGGCAACAGGGAAATCGGTGATTTGTGTAGTCGGTTTAGGCAGCAACGAGC‑CG‑TCAGGGAAAATGCCCCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGa                                                                                                                                                                                                                                    <  2:101332/230‑1 (MQ=255)             ccgcACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGa                                                                                                                                                                                                                                    <  1:794866/239‑1 (MQ=255)                  caTCTGAACTTCAGCCTCCAGTAAAGCGGGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCa                                                                                                                                                                                                                               <  1:617666/239‑1 (MQ=255)                   aTCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCCGGTGAAATTCAGACGGCaa                                                                                                                                                                                                                              >  1:813869/1‑239 (MQ=255)                      tGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTTTCCGGCGCGGAAAAATGCC‑CCCAGGTCAAATTCAAACAGCaaaag                                                                                                                                                                                                                           >  1:349720/1‑237 (MQ=255)                       gAACTTCAGCCTCCAGTACAGCGGGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGa                                                                                                                                                                                                                          <  1:784884/239‑1 (MQ=255)                       gAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGa                                                                                                                                                                                                                          <  1:563302/239‑1 (MQ=255)                            tacccccccAGTACGGGGGGGGTAAAACCATCATTAAAGCGTGTGGAAACAGGAAAATCCTTGATTTGGTTAGTGGGTTTATGCACCAACGAAC‑CG‑TCCCGGAAAATGCCGCTCAACCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTc                                                                                                                                                                                                                     <  2:316432/232‑1 (MQ=255)                            tCAACCGCCAGTACAGCTGGGCTGAAATCATCAGTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTc                                                                                                                                                                                                                     <  1:409275/239‑1 (MQ=255)                             cAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACCTATCCTGATCTTCCAGATAACTGCCGTCACTCCACCGCAGCCCCATCCCCGCGAGGCGGTTTTTTCCCGCGCGTCAAAATGCG‑CCCCGGCCAAATTCCGCCGGCAAACAACCGTcc                                                                                                                                                                                                                    >  2:784884/1‑239 (MQ=255)                             cAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCCCCGCGAGGCGGTTTTCTCCGGCGCGTAAAAAAGCA‑CCCAGGTCACATTAACAGGGCAAACGAcgtgcc                                                                                                                                                                                                                    >  1:604207/1‑234 (MQ=255)                             cAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCCCGCAAAAAAGCG‑CCCCGGTCAAAATCAAACGGCAAACGAATTTcc                                                                                                                                                                                                                    >  2:234029/1‑239 (MQ=255)                                  tCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGGAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCa                                                                                                                                                                                                                               <  1:525376/223‑1 (MQ=255)                                     aGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTTTCCGGCGCGTAAAAATGCG‑CTCAGGTCCAATTCCAACGGAAAACGCCCTTCCTGACCGTa                                                                                                                                                                                                            >  1:156824/1‑239 (MQ=255)                                          aGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCCCGTAAACATGCG‑CCCAGGTCAAATTACGACGGCAAACGACTGCCATGGACGAAACCg                                                                                                                                                                                                        >  2:900337/1‑238 (MQ=255)                                            cgcgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGACAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGGTTTTTCCGGCGCGTAAAAATGCG‑CCCCGGTCAAATTCAAACGGCAAACGAcgggccccggctcttaaacac                                                                                                                                                                                                     >  2:525376/1‑219 (MQ=255)                                            cgcgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGCGGCGGTTTTCTCCGGCGCGCAAAAATGCG‑CCCCGGCCCAATTCAGACGGCTAACGACTGTCCTGGCCGTAACCGAc                                                                                                                                                                                                      >  1:866094/1‑238 (MQ=255)                                            cgcgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CCCAGTGCCAATTCAGACGGCAAAAGAACGGCCTGGCCGTAACCGAc                                                                                                                                                                                                      >  2:1059093/1‑238 (MQ=255)                                            cgcgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CCCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGCAACCGAcc                                                                                                                                                                                                     >  1:91272/1‑239 (MQ=255)                                            cgcgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGGTAAAATGCG‑CTCAGGGCAAATTCAGACGGCAAACGACTGGCCTGGCCGGAACCGAc                                                                                                                                                                                                      >  1:505263/1‑238 (MQ=255)                                            cgcgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGGTTTCTCCGGCCCGTAAAAATGCG‑CCCAGGCCAAATTCAGACGGCAAACAAACGTCCAGGCAGAAACCCAcc                                                                                                                                                                                                     >  2:264508/1‑239 (MQ=255)                                              cgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATTCG‑CCCAGGGCAAATTCAGACGGCAAACGACTGTCCTGGCCGGAACCGAccc                                                                                                                                                                                                    >  1:964066/1‑238 (MQ=255)                                              cgGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGGAACCGAccc                                                                                                                                                                                                    >  1:761600/1‑238 (MQ=255)                                                 cTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGCTTTTTCCGGCGCGTAAAAAAGCG‑CCCCAGCCAAATTTCGAAGGCCAACGACAGTCCCGGGCGACAACGACCC‑aag                                                                                                                                                                                                >  2:1006645/1‑236 (MQ=255)                                                   gAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGGCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGcc                                                                                                                                                                                             <  1:261865/239‑1 (MQ=255)                                                          tcatAAAAGCGAGGGGCAGCATGGAAATCGCTGATTGTGGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGca                                                                                                                                                                                      <  1:173765/239‑1 (MQ=255)                                                                     aGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCCAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCCCAGATGaa                                                                                                                                                                           >  1:925618/1‑239 (MQ=255)                                                                     aGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CCCAGGGCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑CCCCCCCGGTGcaccaaccatgaa                                                                                                                                                                           >  2:809235/1‑231 (MQ=255)                                                                     aGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTCTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTTAGACGGCAAACGACTGTCCTGGCCGTAACCCACCC‑ACCGCCCGTTGaacccaccatgaa                                                                                                                                                                           >  1:585869/1‑230 (MQ=255)                                                                     aGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGCCGGCTTTCTCCGGCCCGTAAAAATCCG‑CCCCGGCCCACTTCATACGGCAAACGCCCGGCCTTGCCCGAACCCACCC‑ACCGCCCGCTGCCCcacaaaaaaa                                                                                                                                                                           >  2:100235/1‑233 (MQ=255)                                                                      gTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGAACGTCCTGGCCGTAACCGACCCAACCCCCCGTTGC‑CCCCAGAAGaaa                                                                                                                                                                          >  2:1005247/1‑239 (MQ=255)                                                                       tGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCCACAGGCAAACGACTGTCCTGGCCGTAACCGACCC‑CGGCACCGTTGaaccaccatggaaa                                                                                                                                                                          >  1:188958/1‑230 (MQ=255)                                                                        caaagcatgaatgggttgtttttGTG‑AGGCGGTTTAGGCCGCACAGGCA‑CG‑TCACGGAAAATGCCGCCCCGCCGCCAACGATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGc                                                                                                                                                                       <  2:937202/219‑1 (MQ=255)                                                                         gcaaacaggAAATCGCTAATTTGTGTCGTCGGTTTATGAAGCAACGAGA‑CG‑GCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACg                                                                                                                                                                        <  1:1055895/231‑1 (MQ=255)                                                                                   acctctttttttgttttgtCGGTTTTAGCACCAACGGAC‑CC‑TCCCGGAAAATCCCCCCCAACCGCCCCATATCCCTATCTTCCAGATAACGGCCGTCACTCCAGCGCAGCCCCATCCCCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACg                                                                                                                                                             <  2:505263/230‑1 (MQ=255)                                                                                     gctcttttttGTTTAGTCGTTTTATGCCACAACGAGAGCG‑AC‑CGGAAAACCCCCCTCACCCGCCACATATCCGGCTCTTCCAGATAACTGCCGTCACTCCAGCGCAGCCCCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGcc                                                                                                                                                           <  2:92786/232‑1 (MQ=255)                                                                                       gCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCAt                                                                                                                                                         <  1:189114/239‑1 (MQ=255)                                                                                        gtttttttggtgttCGGTTTAGGCAG‑AACGGGA‑CGTTCACGGAAAGTGCCCCTCACCCGCCACATTCCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCTCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATc                                                                                                                                                        <  2:80536/226‑1 (MQ=255)                                                                                        cTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCTCAACCCACCC‑AGCGCCCGTTGCCCCCCCGATGAACAGCCAACTTAAcc                                                                                                                                                             >  1:937202/1‑233 (MQ=255)                                                                                           aTTTGTTTAGGCGGTTTATGGAGCAACGAGA‑CG‑TCCCGGAAAAGGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGGGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCaaa                                                                                                                                                     <  2:591439/239‑1 (MQ=255)                                                                                                tgtAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCCCCGATGAAACGCCGAGTTAACGCCATCCAAaataa                                                                                                                                                >  1:185832/1‑239 (MQ=255)                                                                                                  tatgtggTTTATGCAGCAACGGAC‑CG‑TCACGGAAAATGCCGCTCCGCCGCCACATATCCTGGACTTCCGGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGGGGTTTTCTCCGGCGCGTAAAAAGGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAAtt                                                                                                                                              <  2:682565/234‑1 (MQ=255)                                                                                                   aGTCGGTTTAGTCCGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAAtt                                                                                                                                              <  1:886535/238‑1 (MQ=255)                                                                                                     tCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGGCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGAGCCCGTTGCACCACAGATGAAACGCCGGGTTAAAGCCATCCAAAATAATTccg                                                                                                                                           >  1:761771/1‑237 (MQ=255)                                                                                                     tCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCACGTCAAAATGCC‑CTCAGGTCAAATTCAGCCGGCCAACGACGTTCCCGGCCGTAACCGACCC‑AGCGCCCGTTGCCCCACAGCGGAAACCCCGAGCTAAACCCCACAAAaacaactac                                                                                                                                            >  2:206693/1‑234 (MQ=255)                                                                                                          ttATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATTCG‑CCCAGGGCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑ACCGCCCGTTGCACCACAGATGAAACCCCCGATTAACGCCACCaaaaaaaaataccgcccg                                                                                                                                      >  2:688124/1‑229 (MQ=255)                                                                                                          ttATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTTTCCTGGCCGTAACCAACCC‑AGCGCCCGTTGCACACCAGATGAAACGCCGAGTTAAAGCCCTCAAAAAAAAATCGCGCCt                                                                                                                                       >  1:923687/1‑238 (MQ=255)                                                                                                          ttATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCCCGTTAAc                                                                                                                                                              >  1:285491/1‑215 (MQ=255)                                                                                                                  caACGAGA‑GG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGca                                                                                                                                                                                      <  1:904921/183‑1 (MQ=255)                                                                                                                       aga‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCCCAGGTGAAACCCCGAGTTACCGCCATCAAAAATAATTCCCGGCTGGCCTTCCCGTAg                                                                                                                          >  1:889252/1‑238 (MQ=255)                                                                                                                       aga‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGGCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCCCCACAGATGAAACGCCGAGTTAAAGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAg                                                                                                                          >  1:423816/1‑238 (MQ=255)                                                                                                                        ga‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGc                                                                                                                         <  1:23628/238‑1 (MQ=255)                                                                                                                                     aacgcccccccacccGCCACATTTCCTGATCTTCCAGATACCTGCCGTCACTCCAGCGCAGCACCATCCCCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTtcatc                                                                                                              <  2:522785/225‑1 (MQ=255)                                                                                                                                     aaatgccggcactCCACCACATATCCTGATCTTCCAGATAACTGCCGTCATTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCGCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTtcatc                                                                                                              <  1:206693/226‑1 (MQ=255)                                                                                                                                     aaaTGCCCCTCACCCCCCACATTCCCTGATCTCCCAGATAACTGCCGTCCCCCCAGCGCAGCCCCATCCCCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTtcatc                                                                                                              <  2:761600/238‑1 (MQ=255)                                                                                                                                             cTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTGCCGGCTGGCCTTCCTTTAGCCACCTTTCATC‑CACATTaaa                                                                                                    >  1:684085/1‑239 (MQ=255)                                                                                                                                                    gCCACATCTCCTGACCTTCCAGATAACTGCCGTCCCCCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGc                                                                                             <  1:622408/239‑1 (MQ=255)                                                                                                                                                    cccacattcccgattCTTCCAGTTACCTTCCGTCACTCCGGCGCAACCCAATCCCCGCGAGGCGGTTTTCTCCGGCGCGTAAAAAGGCG‑TTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGc                                                                                             <  2:349720/225‑1 (MQ=255)                                                                                                                                                      cacaTATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGcc                                                                                                                                                                                             <  1:675948/142‑1 (MQ=255)                                                                                                                                                          tatCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTtcatc                                                                                                              <  1:332602/217‑1 (MQ=255)                                                                                                                                                                    ttCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCAACGATGAAACCCCGAGTTAACGCCCTCAAAAATAATTCCCGTCCGGCCTTCCTGTAGCCAGCTTTCATC‑AAAATTaaaagctgtctcttaaaaccatctg                                                                              >  2:332602/1‑216 (MQ=255)                                                                                                                                                                         gATAACGGCCGTCCCTCCAGCGCAGCACCATCCCCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑Ttc                                                                      <  1:1006734/239‑1 (MQ=255)                                                                                                                                                                                    tCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTACAAATGCG‑CCCAGGCCCAATTCAAACGGCCAACCACTGACCTTGCCGTAACCGACCC‑CGCGCCCGTTGGCCCCCAGATGGAACGCCGAGTTAACCCCATCACACATAATTCGCGGCTGGCCTTCCCGGAGACCGCTTTCCTCAAACTTTAAATGGGAGG‑GAG‑AACAACCCGGCGGG‑ATTTCCCTGGGaca                                                           >  2:552882/1‑237 (MQ=255)                                                                                                                                                                                      aCTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAAtt                                                                                                                                                                                                                                        >  1:611779/1‑68 (MQ=255)                                                                                                                                                                                        cccgagcgagccCCACCCCCGCGAGGCGGTTTTCCCCGGCCCGTAAAAA‑GCGCCTCAGGTCAAATTCAGCCGGCAACCGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAc                                                       <  1:859290/231‑1 (MQ=255)                                                                                                                                                                                              ccagccCCATCCCCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAACTTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGa                                                <  2:156824/238‑1 (MQ=255)                                                                                                                                                                                                cgccccccccccGCGGGGGGGTTTTCTCCGGGGCGAAAAAAGGGC‑CCCAGGTCAAATTCAGACGGCAAACGACTGGCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGGCTGGCCTTCCTGTAGGCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGAtt                                              <  2:761771/229‑1 (MQ=255)                                                                                                                                                                                                 gcaCCATCACCGCGAGGGGGTTTTCCCCGGCGCGTAAAAATGCC‑CTCGGGTCAAATTCAGACGGAAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTg                                             <  1:447236/239‑1 (MQ=255)                                                                                                                                                                                                  caCCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTTAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCCTCAAAAATAATTCGCGTCTGGCCTTCCCGTAGCCCGCTTTCTTC‑AACCTTCAAT‑TGGGCGGGGGAAAAAACCACCGGA‑TTCTCCCGGGGGACAAAa‑ggggggatt                                             >  2:597800/1‑234 (MQ=255)                                                                                                                                                                                                  caCCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTg                                             >  1:381334/1‑238 (MQ=255)                                                                                                                                                                                                  caCCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CCCAGGTCCAATTCAGACGGCAAAAGACCGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTTTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACACACCGTCGGA‑tt                                                                       >  1:284340/1‑213 (MQ=255)                                                                                                                                                                                                   gccATCGCCGCGGGGGGGTTTTCTCCGGCGCGTAAAAAGGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGAtt                                              <  1:1085508/235‑1 (MQ=255)                                                                                                                                                                                                   cccacccccccggggCTGTTTTCTCCGGGGCGTAAAAATGCG‑CCCAGGTCAAATCCAGACGGCAAACGACTGGCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGAc                                           <  2:381334/226‑1 (MQ=255)                                                                                                                                                                                                   cccATCCCCGGGAGGCGGTTTTCCCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGGCCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGAtt                                              <  1:597800/235‑1 (MQ=255)                                                                                                                                                                                                       tCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCCAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCCCAGGTGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGGTTTCATC‑AACATTAAAGTGGAGC‑GGGAAACAACCCTGCGGATTTCCCCGGGGAAAAAAAc‑ggggaagtgaggaa                                        >  2:310580/1‑227 (MQ=255)                                                                                                                                                                                                       tCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCCCAGATGAAACCCCGAGTTAAAGCCATCAAAAATAATTTCCGTCCGGCCTTCCTTTACCCCGCTTTCATC‑AACATTAAATGTGGAC‑GCGTAACAACCCCTGCGA‑ATCCCCGGGGGAAAAAACAGGAGGAGTGAcaga                                        >  2:422875/1‑236 (MQ=255)                                                                                                                                                                                                        ccccGCGGGGGGGTTTTCCCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATGCAGAGGGAAAACGACTGTCCCGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAAAGCCGAGTTAACGCCATAAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGGGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTaa                                      <  2:632528/237‑1 (MQ=255)                                                                                                                                                                                                            gcgAGGCGGTTTTCTCCGGCGCGTAAAAATTCG‑CTCCGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGGGTTAACGCCCTCAAAAATAATTCGCGTCTGGCCTTCCTGGAACCCACTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAAACCGTCGGA‑TTTTCCGGGGGAACAAAa‑gggaggaagaccgaaaaggg                                  >  2:1088877/1‑221 (MQ=255)                                                                                                                                                                                                             gggggCGGTTTTTCCCGGGCCGTAAAAATGCC‑CTCAGGTCAAATTCAGCCGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGa                                 <  1:1031512/236‑1 (MQ=255)                                                                                                                                                                                                             cacggCTGTTTTCCCCGGCGCCTAAAAACTCC‑TCCAGGACAAATTCAGACGGAACACGACTTGCCTGGCCGTAACCGACCC‑AGCGCCCGTTTCCACACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTGCATC‑AATATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGa                                 <  2:441235/236‑1 (MQ=255)                                                                                                                                                                                                              gAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGa                                 <  1:234029/238‑1 (MQ=255)                                                                                                                                                                                                              gAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGCCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCACCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGa                                 <  1:317543/238‑1 (MQ=255)                                                                                                                                                                                                               aGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCCTCAAAAATAATTCGCGTCTGGCCTTCCTGTAACCAGCTTTCATC‑AACAATAAATGTGAGC‑GAGTAACAACCCGCGGGA‑TTCTCCGTGGGAAAAAAC‑GGCGGGTTGCCCGGaagtggaaa                               >  2:128729/1‑232 (MQ=255)                                                                                                                                                                                                                   ggTTTTCCCCGGCGCGTAAAAATGCG‑CCAAGGGAAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGt                            <  1:639030/238‑1 (MQ=255)                                                                                                                                                                                                                      tttCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGACGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACg                        <  1:650782/239‑1 (MQ=255)                                                                                                                                                                                                                      tttCCCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACg                        <  1:688124/239‑1 (MQ=255)                                                                                                                                                                                                                       ttCTCCGGCGCTTAAAAATGCG‑CTCAGGGCAAATTCAGACGGGAAACGACGGCCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGACGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGt                       <  1:627152/239‑1 (MQ=255)                                                                                                                                                                                                                         ccccGGCGCGTAAAAATGCG‑GTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGGCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTg                     <  1:300499/237‑1 (MQ=255)                                                                                                                                                                                                                                    aaaacTGCC‑CTCAGGTAAAATTCCAACGGAAACCGATTCTCCTTGCCTTAACCGACCC‑AGCCGCCGTTGCAACACAGATGAAACGCCGAGTTAACGCCATCAAAAAGAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATggg          <  2:588134/239‑1 (MQ=255)                                                                                                                                                                                                                                    aaaaaTGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGGCCTTGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATggg          <  1:264508/239‑1 (MQ=255)                                                                                                                                                                                                                                      aaaTGCG‑GTCAGGTCAAATTCAGACGGCAAACGACTTGCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATGGgcg        <  1:900337/239‑1 (MQ=255)                                                                                                                                                                                                                                      aaaGGCC‑CTCAGGTCAAATCCAGGCGGCAAACGCCTGGCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTCCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATGGgcg        <  2:925618/239‑1 (MQ=255)                                                                                                                                                                                                                                            g‑cTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATGGGCGCATCGt  >  1:319392/1‑239 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  TCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGTCGGTTTATGCAGCAACGAGA‑CG‑TCACGGAAAATGCCGCTCATCCGCCACATATCCTGATCTTCCAGATAACTGCCGTCACTCCAGCGCAGCACCATCACCGCGAGGCGGTTTTCTCCGGCGCGTAAAAATGCG‑CTCAGGTCAAATTCAGACGGCAAACGACTGTCCTGGCCGTAACCGACCC‑AGCGCCCGTTGCACCACAGATGAAACGCCGAGTTAACGCCATCAAAAATAATTCGCGTCTGGCCTTCCTGTAGCCAGCTTTCATC‑AACATTAAATGTGAGC‑GAGTAACAACCCGTCGGA‑TTCTCCGTGGGAACAAAC‑GGCGGATTGACCGTAATGGGATAGGTCACGTTGGTGTAGATGGGCGCATCGT  >  NC_000913/365548‑366019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑