BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,932,227	A→T	10.6%	I305F (ATC→TTC)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,227	0	A	T	10.6%	259.8 / 3.3	103	I305F (ATC→TTC)	kup	potassium transporter
Reads supporting (aligned to +/- strand): ref base A (30/59); new base T (10/2); total (42/61)
Fisher's exact test for biased strand distribution p-value = 1.42e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGAC  >  NC_000913/3932006‑3932462

tGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGGTATttcttggtcccttc                                                                                                                                                                                                                                 >  1:33217/1‑227 (MQ=255)

       tGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGGAATTGCCTCGCAGGCGGTTTTTTCTGGCGGCTTTTC‑ATTGAccc                                                                                                                                                                                                                       >  1:484755/1‑237 (MQ=255)

         atatGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCGGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGTACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGCCGGTAGTTGCCTCGCAGGCGTGTTTTTCTGGctgttttccctgtacccg                                                                                                                                                                                                                       >  1:1084909/1‑221 (MQ=255)

           atGGGGCACTTTGGTAAGTTCCCTAGTCGCCTGGCGTGGTTCACCGTCGTATTTCCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCa                                                                                                                                                                                                                   <  1:55597/239‑1 (MQ=255)

            tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGCCGGTAATTGACGCCCAGGCGGTTATCTCTTGGGgcttcccccttaccgccccg                                                                                                                                                                                                                   >  2:285061/1‑224 (MQ=255)

            tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTCTC‑CTTGTCGCGTccg                                                                                                                                                                                                                  >  1:921806/1‑237 (MQ=255)

            tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGGAAATGCCTCGCAGGCGGTTCTTCCTTGCGTCTTTTC‑CTTAAccctgcgg                                                                                                                                                                                                                  >  2:196783/1‑232 (MQ=255)

             ggggCCCTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAgg                                                                                                                                                                                                                 <  1:882192/239‑1 (MQ=255)

              gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTTTCTTTGGGGGCTTTTC‑CTTGTCGCGTCCggg                                                                                                                                                                                                                >  1:199846/1‑238 (MQ=255)

              gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTCCGGATCATCGCCGCACTGGCGACGGTAATTGCTTCGCAGGCGGTTTTTTGTGGCGGCTTTCC‑Atttaacggccacgg                                                                                                                                                                                                                >  1:355733/1‑227 (MQ=255)

               ggccttttGGTAATTTCCCTTTTCCCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGc                                                                                                                                                                                                                <  2:44789/233‑1 (MQ=255)

                 ctcTTTTGTAAGTTCCCTTTTTGCCTGGCGTGGTTCACCGGCGTTTTCCCTTTCTTGACCCTTAATTTCTTCGGTCAGGGAGCGCTTTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCgg                                                                                                                                                                                                              <  2:451965/236‑1 (MQ=255)

                 cccTTTGGTAAGTTCCCTAATCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCTATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCgg                                                                                                                                                                                                              <  1:285061/236‑1 (MQ=255)

                 cACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCgg                                                                                                                                                                                                              <  1:342843/238‑1 (MQ=255)

                   cTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGCCGCCTCTTC‑ATTGTCGCGTCAGGCGGGAc                                                                                                                                                                                                           >  1:451965/1‑239 (MQ=255)

                      tttttttttCCCTTTTCGGCGGGCGTGGTTCACAGTCGTATTTCCTTCCTTGACCCTTAATTACCTCGGTCAGGGGGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTc                                                                                                                                                                                                        <  2:187341/232‑1 (MQ=255)

                         tAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGTTCATCGCCGCACTGGCGACGGTAATGGCCTCGCAGGCGGGTTTCTCTGGGGCGTTCTC‑CTTTAccgggcaaggggtccggcggg                                                                                                                                                                                                     >  2:313970/1‑219 (MQ=255)

                              tCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTTTC‑ATTGTCTCGTCAGGCGGTACGTCTggggact                                                                                                                                                                                                >  1:782664/1‑235 (MQ=255)

                              tCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGCTTTTTCTTGGGGCCTCCC‑ATTGAAGGCTCCGGGGGGACGGCTggggcta                                                                                                                                                                                                >  1:994143/1‑235 (MQ=255)

                                  tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCCCTGGCGCCGGTAATTGCCTCGCAGGCGGTTCTCCATGGCGGCCTATC‑TTTGCGCCGTCAGGCgcgccgccgggggtgttttt                                                                                                                                                                                            >  2:614389/1‑220 (MQ=255)

                                  tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTTTCTCTGGCGCCTTCTC‑ATTGACGCGTCAGGCGGTACGGCTGGGGGAtttt                                                                                                                                                                                             >  1:624079/1‑237 (MQ=255)

                                  tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTCGGGTTTTCCCTTTTCACG‑GTCAGCGGGTACGCCTGGGTCAtttt                                                                                                                                                                                             >  1:897547/1‑237 (MQ=255)

                                         cTGGCGTGGTTCCCCGTCGTTTTCCCTTCCTTGCCCCTTAATTCCTTCGGTCAGGGGGCCGTGTTGTGAAAGAACCCGGGAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑At                                                                                                                                                                                    <  2:274342/239‑1 (MQ=255)

                                          ttgcgggtgttaCCCTCGTATTTCCTTCCTTGACCCTTAATTCCTTCGGTCAGGGATCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATg                                                                                                                                                                                   <  2:624079/228‑1 (MQ=255)

                                           ggCGTGGTTCACCGTCGTATTTCCTTCCTTGACCCTTAATTTCTTCGGTCAGGGAGCGCTGTTGTTAAAGACCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATgc                                                                                                                                                                                  <  1:896012/239‑1 (MQ=255)

                                              gtttttCACCGTCGTTTTTCCTTCCTTGGCCCTTAATTCCTGCGGTCCGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCa                                                                                                                                                                               <  2:71031/235‑1 (MQ=255)

                                               tGGTTCACCGTCTTATTACCTTCCTTGTCCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCa                                                                                                                                                                               <  1:147058/238‑1 (MQ=255)

                                                   tccccGTCGTATTTCCTTCCTTGACCTTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCatta‑tt                                                                                                                                                                         <  1:124024/236‑1 (MQ=255)

                                                     tcctgcgtTTTACCTTCCTTTCCCCTTAATTACGTCCGTCAGGGGGGGCTTTTGTTAAAGAACCCGGAAGCAATTAAGACCCCGTTCTTCCTGTTGGCCCCGGACTGGGCGCTGATCCCGCTGCTGATCAGCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTca                                                                                                                                                                       <  2:659386/234‑1 (MQ=255)

                                                        gtggttttgccttccttGACCCTTAATTACTGCGGGCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCacac                                                                                                                                                                    <  2:205119/230‑1 (MQ=255)

                                                           gtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGGCAGGCGGTACGTCTGGGATATTTTTTGCCG‑GTTCGCATTA‑TTTACACCt                                                                                                                                                                  >  1:590864/1‑238 (MQ=255)

                                                            tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATTTCTGGCGGCTTTCC‑ATTGAGGCGTCAGGGGGGACGGCCTGGGTATTTTTCTCCgcacggccctttttccaaccc                                                                                                                                                                   >  1:494517/1‑218 (MQ=255)

                                                            aaaggaccgcctttgcccTTTATTTCTTCGGGCCGGGGGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTcc                                                                                                                                                                <  2:302642/224‑1 (MQ=255)

                                                                aCCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTCTC‑ATTTTCTGGGCCGGGGGGACGTCTGGGGTATTTTTCGCCG‑CTGCGCCTTA‑TTCACACCCCCGcaa                                                                                                                                                            >  2:628445/1‑239 (MQ=255)

                                                                aCCTTCCGTGCCCCTTTATTTCTTCGGTCAGGGTGCGCTGTTTTTAAAGCACCCGGGAGCGGTTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGaaa                                                                                                                                                            <  2:52894/239‑1 (MQ=255)

                                                                 ccttccttGCCCCTTAATTACTTCGGTATGGGAGCGCTGTTGGTAAAGAACCCGGCAGCGATGAAAAACCCCTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCCGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAAt                                                                                                                                                           <  2:352567/239‑1 (MQ=255)

                                                                 ccttccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑CTTGACGCGTCAGGCGGTACGTCTGGGGTATTTGTCGCCG‑ATGCGCATTT‑TTCACACCTCCGaaaa                                                                                                                                                           >  1:403041/1‑238 (MQ=255)

                                                                    tccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATTTCTTGCGTCTTCTC‑ATTTACGCGTCCGGCGGGACGTCTGGGGTATTTGTCGCCG‑ATGGGCCTTA‑TTTACACCTCCGCAATggg                                                                                                                                                        >  1:351792/1‑238 (MQ=255)

                                                                    tccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTCTC‑ATTGACCCGTCAGGCGGGACGCCTGGGGTATTTGTCGCCG‑ATGCGCATTA‑TTTCCACCTCCGCAATAGa                                                                                                                                                        >  1:432342/1‑239 (MQ=255)

                                                                    tccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGCTATCTCTGGGGGCTTCTC‑ATTGTCGCGTCCGGCGGGACGTCTGGGGTATTTGTCGCCG‑GTGCGCATTA‑TTcaaccccccaacacgga                                                                                                                                                        >  1:989025/1‑224 (MQ=255)

                                                                      cttGCCCCTTAATTTCTTGGGTCAGGGAGCGCTGTTTGTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGt                                                                                                                                                      <  2:35325/239‑1 (MQ=255)

                                                                      cttGCCCCTTAATTTCTTCGGTCAGGGAGCGCTGTTGTTAACGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGt                                                                                                                                                      <  2:496573/239‑1 (MQ=255)

                                                                      cttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTC‑ATTGTCGCGTCAGGCGGGACGCCTGGGGTATTTTTTCCCG‑TTTCGCctta‑tttcacccccccaaaaggag                                                                                                                                                       >  1:863757/1‑220 (MQ=255)

                                                                       gattggtataagagacagggTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGt                                                                                                                                                      <  1:114844/220‑1 (MQ=255)

                                                                            ccTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGc                                                                                                                                                                                         <  1:341932/200‑1 (MQ=255)

                                                                             tttatttttttCGGTCAGGGGGCTCTGTTGTTAAAGAACCCGGAAGCGATTAAGGACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCa                                                                                                                                               <  2:484755/230‑1 (MQ=255)

                                                                             cTTTATTGCTTCGGTCAGGGGGCGCTGTTGTTAAAGAACCCGGAAGGGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCa                                                                                                                                               <  2:199846/239‑1 (MQ=255)

                                                                             cTTAATTTCTTCGTTCCGGGGGCCCGTTTGTTAAAGAACCCGGAAGCGATTAAATACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCa                                                                                                                                               <  2:403041/239‑1 (MQ=255)

                                                                             cTTAATTCCTTCGGTGAGGGAGCGCTGTTGTGAAAGAACCCGGAAGCGATTAAGAACCCTTTCTTCCTGTTGGCACCGGACTGGGCGCTGGTCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCa                                                                                                                                               <  1:1002533/239‑1 (MQ=255)

                                                                             cTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCa                                                                                                                                               <  2:73926/239‑1 (MQ=255)

                                                                             cTTAATTACCTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCa                                                                                                                                               <  1:628445/239‑1 (MQ=255)

                                                                               tAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGGTATTTGTCGCCG‑ATGCGCCTTA‑TTCACACCCCCGGAATGGGGTCAGGGCaaa                                                                                                                                             >  1:35325/1‑239 (MQ=255)

                                                                               tAATTACCTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCaaa                                                                                                                                             <  1:694683/239‑1 (MQ=255)

                                                                                aaTTACTTGGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAGCCCGGTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCaaa                                                                                                                                             <  1:846042/238‑1 (MQ=255)

                                                                                aaTTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTTGCGTCTTCTC‑ATTGACGCGTCAGGCGGGACGGCTGGGATATTTGTCGCCG‑ATGCGCATTT‑TTTACACCCCCGCAATggggg                                                                                                                                                      >  1:302642/1‑226 (MQ=255)

                                                                                     cTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCCTCTC‑ATTGACGCGTCAGGCGGGACCTCTGGGGTATTTGTTGCCG‑ATTCGCCTTA‑TTCTCACCCCCCAAATTGAGTCAGGGCCAAtata                                                                                                                                         >  1:257207/1‑237 (MQ=255)

                                                                                      ttCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATACCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGGACGCCTGGGGAATTTTTGGCCG‑ATGGGCCTTA‑TTTCCCCCCCCGaaaatggacccggggaaaacaaata                                                                                                                                      >  2:962676/1‑217 (MQ=255)

                                                                                        cGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTTTCTCTGGCGTCTTCTC‑ATTGACCGGTTAGGCGGGAGGTCTGGGATATTTGTCGCCG‑ATGGGCCTTA‑TTTCCACCTCCGAAATGGAGGCAGGGCaaaatttaatt                                                                                                                                     >  1:682074/1‑230 (MQ=255)

                                                                                          gTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑CTTGGAGCGTCAGGCGGTGCGGCTGGGGTATTTTTGGCCC‑CTAGGGATTA‑TTCTCCCACCCGCAATGGGGTCAGGGCAAATCTATATTccc                                                                                                                                  >  2:846042/1‑238 (MQ=255)

                                                                                          gTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTCTC‑ATTGACCCGGCAGGCGGGACGTCTGGGGTATTTGTCGCCG‑CTGCGCCTTT‑TTCACACCTCCGAAATGGAGGCAGGGCCAATCTATATTc                                                                                                                                    >  1:355315/1‑237 (MQ=255)

                                                                                           tCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTc                                                                                                                                                                                                        <  1:1043183/170‑1 (MQ=255)

                                                                                               ggAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGAACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCCGGCGTCTTCTC‑ATTGTCCCGTACGGCGGGGCGGCTGGGGTGTTTTTCGGCGCGTGC‑CATTA‑TTCTCACCCCCGCAAAGGGGTTGGGGCAAAATTATATTCCGTTtgt                                                                                                                             >  2:754988/1‑239 (MQ=255)

                                                                                                  gcgcTGTTGTTAAAGAACCCGGGAGGGTTTAAGCACCCGTTCTTCCTGTTGGCCCCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGa                                                                                                                           <  2:564629/238‑1 (MQ=255)

                                                                                                  gccttttttttAAAGACCCCGGAAGCGATTAAGACCCCGTTCTTCCTGTTGGCCCCGGCCTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGa                                                                                                                           <  2:355315/229‑1 (MQ=255)

                                                                                                    gcTGTTGTTAAAGAACCCTGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACt                                                                                                                        <  1:956936/239‑1 (MQ=255)

                                                                                                    gcTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGAGATTTGTCGCCC‑GTTGGCATTATTTCAC‑CCCCCGCAAGGGGGTCAGGGGaagacccgtctctttacaacacac                                                                                                                        >  2:114844/1‑217 (MQ=255)

                                                                                                      tgttgtTAAAGACCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTGGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTgg                                                                                                                      <  1:313970/239‑1 (MQ=255)

                                                                                                      tgttgtTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTgg                                                                                                                      <  1:962676/239‑1 (MQ=255)

                                                                                                      tgttgtTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTgg                                                                                                                      <  1:187016/239‑1 (MQ=255)

                                                                                                      gtgtgttAAAGGACCCGGAAGCGATTAAGACCCCGTTCTGCCTGGTGGCCCCGGCCTGGGCGCTGATCCCGCTGCTGATCATCGCCGGATTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTgg                                                                                                                      <  1:500778/236‑1 (MQ=255)

                                                                                                       ggtgttAAAGAACCCGGAAGCTATTAAGAACCCGGTCTTCCTGGTGGCCCCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTgg                                                                                                                      <  1:946744/236‑1 (MQ=255)

                                                                                                         tgttAAAGACCCCGGAAGCAATTTAGAACCCGTTCTTCCGGTGTGCCCCGGACTGGGCGCTTATCCCGCTGGGGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATg                                                                                                                   <  2:863757/239‑1 (MQ=255)

                                                                                                                  aCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTTACGCGTCAGGCGGTACGTCTGGGGTATTTTTCGCCC‑GTGCGCATTA‑TTCACCCCCCCCAAATGGAGGCAGGGGAAAACCAAATTCCGGTTTTGaaaaggaggcctctagtg                                                                                                          >  2:382388/1‑223 (MQ=255)

                                                                                                                   cccGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGGCAGGCGGGACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGGAAATCTATATTCCGTTTTTGAACTGGATGCTCTTTGtg                                                                                                          >  1:789370/1‑237 (MQ=255)

                                                                                                                        aaGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTTGCGTTTTCTC‑ATTGACGCGTCAGGGGGGACGTCTGGGAGATTTTTTGCCG‑GTCCGTCTTT‑TTTACCACTCCGAAAAGGAGCTAGGGCAAATCTATATTCCGTTTGTGGACTGGagcgccttggacgggggg                                                                                                    >  2:929822/1‑222 (MQ=255)

                                                                                                                            gATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCgtga                                                                                                <  1:382388/239‑1 (MQ=255)

                                                                                                                                        gTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGGTTTCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTGCGTCTGGGGACTTTGTTGCCG‑CTTCGCATTA‑TTCCCACCCCCGAAATGGAGGCCGGGCAAATCTATATTCCGCTTGTGAACAGGAAGCTCTCTGTCCCgggaggggttggtgttttgc                                                                                    >  2:589864/1‑221 (MQ=255)

                                                                                                                                              ccTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGCCTTCTC‑CTTGCCGCGTCCGGCGGGACGGCTGGGGTATTTGTCGCCG‑ATGCGCATTA‑TTTCCCCCTCCCCAATGGGGTCAGGGCAAAACTATATTTCGTTTGTGAACTCGAGGCTCTATTTAGCGGGCTGGATTGTGTGTGTCTGCtttt                                                                              >  2:281622/1‑238 (MQ=255)

                                                                                                                                                tGTTGGCCCCGGACTGGGCGCTGATCCCGCTGCTGATCATCGGCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAg                                                                            <  1:929822/239‑1 (MQ=255)

                                                                                                                                                ggTTGGGCCCGTACTGGGCGCTTAGCCCCCGGGTGCTCGTCTCCGCTCTGGCGACGGGAATTGCCTCGCAGGTGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAg                                                                            <  2:75483/238‑1 (MQ=255)

                                                                                                                                                          ggACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTTACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCCTTTTTGAACTGGATGCTCCATGTCGCGGTCGGGATTGTGATTGTATGCTTTTAGCCCTCCCGc                                                                   >  1:250008/1‑238 (MQ=255)

                                                                                                                                                                 gcgcTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTTTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTgg                                                            <  1:196783/238‑1 (MQ=255)

                                                                                                                                                                          ccctttcttttcatcGCCGCCTTGGCGACGGTATTTGCCTCGTCGGCGGTTATCTTTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACCGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTa                                                  <  2:432342/229‑1 (MQ=255)

                                                                                                                                                                          ccGCTGCTGATCAGCGCCGCTCTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTa                                                  <  1:614389/239‑1 (MQ=255)

                                                                                                                                                                          ccGCGGCTGATCATCGCCGGACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTa                                                  <  1:191222/239‑1 (MQ=255)

                                                                                                                                                                          ccGCGGCTGATCAGCGCCGCACTGGCGGCGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTa                                                  <  2:782664/239‑1 (MQ=255)

                                                                                                                                                                          cagctgctTATCATCGGCGCACTGGCGAAGGGAAATGCCTCGCAGGCATTTATCTCGGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTATGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCGCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTa                                                  <  1:1079176/237‑1 (MQ=255)

                                                                                                                                                                              tgctgATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGt                                                                                                     <  1:459510/184‑1 (MQ=255)

                                                                                                                                                                                   atcatcGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTCTC‑ATTGGCGCGTCAGGCGGGACGGCTGGGAGATTTGTCGCCG‑ATGCGCATTA‑TTCCCACCTCCGAAATGGGGGTAGGGCAAAACTTTATTCCGTTTGGTAACCGGGTGCTCCATGGCGCGGGGCGGATTGTGATTGTGCCCTTTGAGGACCCCCCAAACCCggcggcggctgaacggggtgg                                         >  2:706140/1‑227 (MQ=255)

                                                                                                                                                                                    tcatcGCCGCACTGGGGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCg                                        <  1:268568/239‑1 (MQ=255)

                                                                                                                                                                                    ctaccgcccccatggtggcGGTGATTGCTTCGTCGGCTGTTATTCTTGGCTGCTTTTC‑ATTTACGCCTCAGGCGGTACGTTTGGGATATTTGTCGACG‑ATGCGCTTTG‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTTAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCg                                        <  2:573279/221‑1 (MQ=255)

                                                                                                                                                                                         gccgcACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGAc                                   <  1:178679/239‑1 (MQ=255)

                                                                                                                                                                                             cACTGGCGACGGTAATTGCCTCGCGGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTcc                                                                      <  1:904436/200‑1 (MQ=255)

                                                                                                                                                                                             cACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCCCACCTCCGAAATGGAGTCCGGGGAAAACTATATTCCGTTTGTGAACTGGATGACCTTATTCGCGGGCGTGGTTGGGATTTTTAGGTTTGAGCACTCCCCCAAACTACGGGGGGCGCACGGGGTTgggggagcggga                               >  2:408476/1‑231 (MQ=255)

                                                                                                                                                                                                 ggcggcGGTTATTTCCCCGGAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCa                           <  2:682074/239‑1 (MQ=255)

                                                                                                                                                                                                         tAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCt                    <  1:125883/238‑1 (MQ=255)

                                                                                                                                                                                                           gtttcttcggtggCGGTTTTCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGAc                 <  2:897547/228‑1 (MQ=255)

                                                                                                                                                                                                                   gCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGGACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGCAATGGAGTCAGGGCAAATCTATATTCCGTTTTTGAACTGGCTGCTCTATGTCGCGGTCGTGGTTGTGATTGTCAGCTTTTAGCACCCCCGCAACCTGGCGGCGGGGTACGGGGTTGCGGGGGCCGGGACCCTTGGGCTGGCggcctat          >  2:774012/1‑232 (MQ=255)

                                                                                                                                                                                                                   ccgggCCGTTTTTTCTGGCGTTTTCTC‑ATGGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATtc         <  2:921806/236‑1 (MQ=255)

                                                                                                                                                                                                                           ttATCTCCGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCTGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGAc  <  1:281622/238‑1 (MQ=255)

TGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTC‑ATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCG‑ATGCGCATTA‑TTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGAC  >  NC_000913/3932006‑3932462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`