BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,054,191	T→G	10.0%	T363P (ACC→CCC)	glnG ←	fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,054,191	0	T	G	10.0%	281.8 / 2.4	128	T363P (ACC→CCC)	glnG	fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand): ref base T (45/60); new base G (17/2); total (66/62)
Fisher's exact test for biased strand distribution p-value = 2.65e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TGCGTATGTCGCAACGCGGTCGTCAGTAACGTCCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCG  >  NC_000913/4053953‑4054426

tGCGTATGTCGCAACGCGGTCGTCAGTAACGTCCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCACATCCTGAATCAACACTTCCTGCCCGCCGGCCATCCCCGTTAGCAACCGCCAGgt                                                                                                                                                                                                                                                 >  1:1055892/1‑239 (MQ=255)

            aaCGCGGTCGTCAGTAACGTCCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCACCT‑g                                                                                                                                                                                                                                    >  1:756046/1‑239 (MQ=255)

            aaCGCGGTCGTCAGTAACGTCCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCCCCGCAACCGTTGATCCAAACAGTTCGCCGGGCAAATCCTGAATCAAAACTTCCTGCCCGGCGGCCATCACCCTTAGCCCCCGGACGGGGTTTTcccaccg                                                                                                                                                                                                                                     >  2:532463/1‑234 (MQ=255)

                 tgtcgtcAGTACCGTCCGGCCCAGCTCGGGGCGGGCTTCGGAAAGCAGTTTTTGATGACCGGAACGCAGCGCTCTGGCTGCCCACTGCGCAAGAAGCGTCGCCAAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCa                                                                                                                                                                                                                               <  2:844634/238‑1 (MQ=255)

                  gttctcAGTAACCTCCGCTTCAGCTTTGGCTGCGCTTCGGAAAGCAAATTTTGATGGCCGGAACGCAGCGGTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCAc                                                                                                                                                                                                                              <  2:17086/235‑1 (MQ=255)

                  gtcgtcAGTAACGTCCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTGGCCGGGCAAATCCTGAATAACCACTTCCTGCACGGCGGCCATCCCCCTTCCCCAGAGGAAGGGGATTTCCAGCT‑Ggcgccc                                                                                                                                                                                                                              >  2:651546/1‑237 (MQ=255)

                            aCGTCCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGc                                                                                                                                                                                                                   <  1:791303/239‑1 (MQ=255)

                                 cGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCT‑GGGCCACGTTCCC‑CGGCCAcc                                                                                                                                                                                                               >  1:251959/1‑237 (MQ=255)

                                 cGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGCCCATCACCGTTAGCCAGCGGCAGGAGTTTTCCAGCG‑GGCGCCCgggtgccggccccg                                                                                                                                                                                                                >  2:584696/1‑225 (MQ=255)

                                    tCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCCCGCCAg                                                                                                                                                                                                           >  1:782180/1‑239 (MQ=255)

                                     ccAGCTTTGGCTGCGCTTCGGAAATCAGATTTGGATGACCGGAACGCAGCGCTTGGTCTGCCCACTGCGCAAGAAGCGGCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGa                                                                                                                                                                                                          <  1:584696/239‑1 (MQ=255)

                                     ccAGCTCTGGCTTCGCGTCGGAAAGCAGATTTTGATGACCGGAACGCCGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGa                                                                                                                                                                                                          <  1:229185/239‑1 (MQ=255)

                                     ccAGCTCTGGCTGCGCTTCTGAAAGCAGATTTGGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGACCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACTCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGa                                                                                                                                                                                                          <  1:651546/239‑1 (MQ=255)

                                     ccAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGa                                                                                                                                                                                                          <  1:957382/239‑1 (MQ=255)

                                              gCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCCGCGGCAGGGGTTTTCCAGCG‑CGCGCACCTTGCCGGGGCCACCCCAAACG‑GTCaga                                                                                                                                                                                                 >  2:229185/1‑239 (MQ=255)

                                              gCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTCAATCAACACTTCCTGCCCGGCGGCCATCACCGCTAGCCAGCGGCAGGTGTTTTTCAGCC‑GGCGCACGTTGCC‑TGGCCACCCCCGACGCGGCaga                                                                                                                                                                                                 >  1:541088/1‑239 (MQ=255)

                                                  cgcTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGCGTTTTCCGGCT‑GGCGCACGTTGCC‑TTGCCCCGCCAGACGCGTCAGacca                                                                                                                                                                                              >  1:802852/1‑238 (MQ=255)

                                                   gcTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAACT‑GGCGCACGTTGCC‑TGGCCACGcacgacccgcccgatca                                                                                                                                                                                              >  1:432653/1‑221 (MQ=255)

                                                       cGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCCCGCCAGACCCGTCAGAGCAGCTTcc                                                                                                                                                                                        >  1:62987/1‑238 (MQ=255)

                                                               aGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCg                                                                                                                                                                                 <  1:32297/238‑1 (MQ=255)

                                                                ggtttttattcccGGAACGCCGCGCTCTGTCTGCCCACCGCGCAAGAAGCGTCGCCCCGCTGGCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGa                                                                                                                                                                               <  2:836355/228‑1 (MQ=255)

                                                                 atttgtatgaCCGGAACGCCGCGCTCTGTCTGCCCACGGCGCAAGAAGCGTCGCCCGGCTGGCCGGTTGCATTTGCGAAGTACTCGCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGa                                                                                                                                                                               <  2:476340/232‑1 (MQ=255)

                                                                  ttttGAAGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTGCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGa                                                                                                                                                                               <  1:323403/237‑1 (MQ=255)

                                                                   tttGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAACCAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGGTTTCCAGCTGGGCGCCCG‑TGCC‑TGGCACCGCCAGCCGCGGCAGGACCGCCTCGGGTTCCGGCTgg                                                                                                                                                                            >  2:213635/1‑238 (MQ=255)

                                                                                      cgcTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGGTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCCGACGCGTCAGAGCCGCTTCGGTTTCCGGATGCAGTAAATTCGCtttcacg                                                                                                                                                          >  1:453576/1‑233 (MQ=255)

                                                                                       gcTCTTTCTGCCCCCTGCCCAAGAAGCTTCCCCCAGCTTTCCGGTTGCCTTTGCGAAGTTCTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTTAATCAACCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGcc                                                                                                                                                        <  2:782180/239‑1 (MQ=255)

                                                                                             tctgCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCCGTAACTTCCCTTCTACGCCCAGtt                                                                                                                                                   >  1:695724/1‑238 (MQ=255)

                                                                                              ctgCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTcg                                                                                                                                                 <  1:2643/239‑1 (MQ=255)

                                                                                                  cccctggccAAGAAGCCTCGCCCAGCTGGCCGGTTGCATTTGCGAAGTTCCCCCCGCAACCGTTGATTCAAACAGGTCGCCGGGCAAATCCTGAATCACCACTTCCTGCCCGGCGGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTcgcgcg                                                                                                                                             <  2:724637/231‑1 (MQ=255)

                                                                                                     cTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTcggccgcg                                                                                                                                           >  1:238769/1‑238 (MQ=255)

                                                                                                     cTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACCTTGCC‑TGGCCACGCCAGACCCGGCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCCCTTCTACCCCCCGTTCCCGCgggg                                                                                                                                          >  2:1004272/1‑239 (MQ=255)

                                                                                                     cTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCCGTTcgcgcgcg                                                                                                                                           >  1:836596/1‑238 (MQ=255)

                                                                                                     cTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGCGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGGGCAGCTTCGGTTTCCCGAGGCAGTAACTCCGCTTTTACCCCCAGTTcgcgcgcg                                                                                                                                           >  1:607719/1‑238 (MQ=255)

                                                                                                      tGCCCAAGAAGCGTCCCCCAGCTGTCCGGTTGCATTTGCTAAGTCCTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGc                                                                                                                                         <  2:65617/239‑1 (MQ=255)

                                                                                                       gggcAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCa                                                                                                                                        <  1:1004272/237‑1 (MQ=255)

                                                                                                       gcgcAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTTCC‑TGGCCACGCCAGACGCGTCAGATGCGCTTCGGTTTCCGGATGCCGTAACTTCCCCTCTACGCCACGTTcgcgcggcgga                                                                                                                                        >  2:290897/1‑234 (MQ=255)

                                                                                                       gcgcAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCCCACGTTGCC‑CGGCCCCCCCAGACCGGTCAGAGCACCTTCGGTTTCCGCATGCGGTAACTTGCCTCTTACGCCCATTGcgcgcgcgcga                                                                                                                                        >  2:888587/1‑236 (MQ=255)

                                                                                                         gcAAGAAGCGTCGCCCCGCTTTCCGGTTTCTTTGGGGAAGTGCCCCCCGCAACCGTTGATTCAAACAGTTCCGCGGGCCAATCCTGAATCCACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAc                                                                                                                                      <  2:471834/239‑1 (MQ=255)

                                                                                                         gcAAGAAGCGTCGCCCAGCTGGCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACGTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAc                                                                                                                                      <  1:180604/239‑1 (MQ=255)

                                                                                                                  gTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGGaaaaa                                                                                                                            >  1:105877/1‑239 (MQ=255)

                                                                                                                            ggTCCGGTTGCATTTGCGAAGTACCCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGgcgcgc                                                                                                                   <  1:877070/237‑1 (MQ=255)

                                                                                                                              tCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCCATTCCCGCGCGGCAAC‑CTGTAAAAAATAGCGCCCCa                                                                                                                 >  2:954149/1‑238 (MQ=255)

                                                                                                                              tCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCCCGCGGCAAC‑CTGCTAAAAATGGCGCGcccg                                                                                                                >  1:136314/1‑237 (MQ=255)

                                                                                                                              tCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGGTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGCCCGAGCAGCTTCGGTTTCCGGAGGCGGGAACTTCGCTTCTACGCCCAGTTCGCGACCGGCCAC‑CTGTaaaaaagagcggccaaa                                                                                                                >  2:941596/1‑228 (MQ=255)

                                                                                                                              tCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCCGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCT‑GGCGCCCGTTGCC‑TGGCCACCCCCGCCCCGCCCAGGCGGCTTCGGTTTCCGGATGCAGTACATTTCCTTCTTCCCCCAGTTCCCGGCCGGCAAC‑CTGGAAAAAAAGGGACGCCAg                                                                                                                >  2:672782/1‑239 (MQ=255)

                                                                                                                               ccgtgtggattttggatttgctctCCGAAACCGTTGATGCAAACAGTTCGCCGGACAAATCCGGGAGCAACCCTTCCTGCCCGGCGGCCAGCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTACCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGa                                                                                                               <  2:149194/219‑1 (MQ=255)

                                                                                                                                   ttGCATTTGCGAAGTACTCCCCGGAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑gg                                                                                                           <  1:716612/238‑1 (MQ=255)

                                                                                                                                        tttGCGAAGTACTCTCCGCCACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACTCTTCCTGCCCGGCGGCCAGCACCGTTAGCCGGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAata                                                                                                     <  1:520079/239‑1 (MQ=255)

                                                                                                                                        tttGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑CGGCCCCCCCAAACGCGCTCGAGCGACTTCGGGTTCCGGATTCCGCAACTTCGCTTCCACGCCCAGTT‑GCCCGCGGGAACACTGTaaaaaaacgcccgcagaacaggggaaa                                                                                                      >  2:1005855/1‑218 (MQ=255)

                                                                                                                                                    ctccccccaCCGTTGATTCGAGCAGTTCGCCGGGACAATCCTGAACCAACCCTTCCGGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑CGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGt                                                                                         <  2:130506/231‑1 (MQ=255)

                                                                                                                                                       ccGCAACCGTTGATTCAAACCGGTCGTCGGGCAAATCCTGAACCAACCCTTCCTGCCCGGCGGCCATCACCGTTATCCAGCGGCAGTTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGTCGTTcg                                                                                      <  1:140434/239‑1 (MQ=255)

                                                                                                                                                        cGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGa                                                                                                               >  1:1070191/1‑214 (MQ=255)

                                                                                                                                                        cGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGa                                                                                                               <  2:1070191/214‑1 (MQ=255)

                                                                                                                                                          gtttaagggccgggggtgggcccccGGGCAAATCCCGAACCAACCCTTCCCGCCCGGCGGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCgcgc                                                                                   <  2:725311/216‑1 (MQ=255)

                                                                                                                                                           aaCCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGGTGCAGGAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATAGCGCGCCAGAC‑GGGGAAACACTTCCCgcacttccgccg                                                                                  >  2:838222/1‑228 (MQ=255)

                                                                                                                                                           aaCCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCC‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGCGCAGCTTCGGTTTCCGGATGCAGGAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CCGTAAAAAAATGCGCGCCACACAGGGGaaacttcccccaccgtccccaa                                                                                   >  2:189699/1‑219 (MQ=255)

                                                                                                                                                           aaCCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCT‑GGCGCCCGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGCTGTAGTAACTTCGCTTCACCGCCCAGTTCGCCCGGGGCAAC‑CTGTAAAAAATGGGCGGCCCGAA‑CGGGAATAACTTtccccagttacgcca                                                                                  >  2:452504/1‑227 (MQ=255)

                                                                                                                                                                ttGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCCAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATATTCCCGACGTTCGCGCAgaggg                                                                             >  1:364046/1‑238 (MQ=255)

                                                                                                                                                                ttGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAgcggc                                                                             >  1:553915/1‑239 (MQ=255)

                                                                                                                                                                     tCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCCACGTTCGCGCAGCGGCGCCAg                                                                        >  1:933948/1‑239 (MQ=255)

                                                                                                                                                                            gTTCGCCGGGCAAATCCTGAAGCAACTTTTCCCGCCCGGGGGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGTAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAAcg                                                                 <  2:407068/239‑1 (MQ=255)

                                                                                                                                                                             ttCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTAGCGCAGCGGCGGCAGATGAAcgc                                                                >  1:90652/1‑239 (MQ=255)

                                                                                                                                                                               ccccGGGCAAATCCTGTATCCACCCTTCCCGCCCGGCGGCCATCACCGTTAGCCCGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGGTTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCgg                                                              <  2:311363/237‑1 (MQ=255)

                                                                                                                                                                                 ccGGGCAAATCCGGGATCACCCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCATGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGAt                                                            <  1:969273/239‑1 (MQ=255)

                                                                                                                                                                                  cGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGCCGTTCGCGCAACggcggccgcggccccttata                                                            >  1:725311/1‑224 (MQ=255)

                                                                                                                                                                                     gCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAAGGCGGATAACg                                                        >  1:311363/1‑239 (MQ=255)

                                                                                                                                                                                         atccgggaccAACCCTCCCCGCCCGGGCGCCATCCCCGGTAGCGCGCGGCAGGTGTTTTCCAGCT‑GGCTCACGTTGCC‑TGGTCACGCCAGACGCGTCAGAGCAGCGTCGGTTTCCGGATGCAATAACTGCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCa                                                    <  2:777102/230‑1 (MQ=255)

                                                                                                                                                                                         acccctaaaacacccTTCCGGCCCGGGGGCCATCCCCGTTACCCAGGGGCGGGTGTTTTCCCGCT‑GGCGGACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCa                                                    <  2:695724/225‑1 (MQ=255)

                                                                                                                                                                                           cccgAATCAACCCTTCCTGCCCGGCGGCCATCCCCGGTAGCCAGCGGCAGGGTTTTTCCAGCT‑GGCGCCCGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAgg                                                  <  1:672782/236‑1 (MQ=255)

                                                                                                                                                                                           ccTGAATCCACCTTTCCTGCCCGGCGCCCCTCCCCTTTGGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAgg                                                  <  2:756046/239‑1 (MQ=255)

                                                                                                                                                                                           ccTGAACCAACCCTCCCCGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCGGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAgg                                                  <  2:889253/239‑1 (MQ=255)

                                                                                                                                                                                              gaactaacctttCCTGCCCGGGGGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGTAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCgg                                               <  1:452504/229‑1 (MQ=255)

                                                                                                                                                                                              gAATCAACCCTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCgg                                               <  1:290897/239‑1 (MQ=255)

                                                                                                                                                                                                cgcAACGCTTCCGGCCCGGCGGCCAGACCCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCgg                                               <  1:297132/235‑1 (MQ=255)

                                                                                                                                                                                                   aaCACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTccc                                                                                              >  2:822488/1‑187 (MQ=255)

                                                                                                                                                                                                   aaCACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTccc                                                                                              <  1:822488/187‑1 (MQ=255)

                                                                                                                                                                                                     cacTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGGGCGGCCAC‑CTGTAAAAAATGGCGGGCCAAAC‑GGGGAAAATCTTCCCACCGTTCGCCCACAGGCGGCGGATGAAAACGGATAACATTCAGGCGGGGGaaaa                                        >  2:850140/1‑237 (MQ=255)

                                                                                                                                                                                                        ttCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGAt                                     <  1:353557/239‑1 (MQ=255)

                                                                                                                                                                                                         tCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACCCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCCCTTCTACGCCCAGTTCGCGCGCGGCCAC‑CCGTAAAAAATGGCCCCCCAGAC‑GGGGAATATCTTCCCGACGTTCGCCCCGCGGCGGCCGCTAAACGCGGGAAACGTTCaccggcggggaacaaa                                     >  2:793236/1‑223 (MQ=255)

                                                                                                                                                                                                         tCCTGCCCGGCGGCCATCACCCTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATc                                    <  1:23725/239‑1 (MQ=255)

                                                                                                                                                                                                             gCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGGTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCa                                <  1:946856/239‑1 (MQ=255)

                                                                                                                                                                                                             gCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCACCAGCA‑CGGGAATATCTTCCCGACCTTTGCCCAGCGGCGGCCACTGAAACCCGCAAAAGTTCAGGCGGGGGCAAAGagactcc                                >  2:1043886/1‑233 (MQ=255)

                                                                                                                                                                                                             gCCCGGCCGCCATCCCCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCa                                <  1:693118/239‑1 (MQ=255)

                                                                                                                                                                                                             cgccGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCa                                <  1:353693/237‑1 (MQ=255)

                                                                                                                                                                                                             ccccGGCGGCCTTCCCCGTTAGCCCGCGGCGGGGTTTTTCCAGCT‑GGCGCCCGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAACATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCa                                <  2:432653/238‑1 (MQ=255)

                                                                                                                                                                                                               ccGGGGGCCCTCCCCGTTGGCCGGGGGCGGGTTTTTTCCAGCT‑GGGGCACGTGGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACg                              <  2:932892/239‑1 (MQ=255)

                                                                                                                                                                                                                  gcggcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCCGAGCAGCTTCGGTTTCCGGATGCAGTAACTTAGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATAAACCCGGATAACGTTTAGGCGTGGGAACAGATCCTCACggga                           >  1:161155/1‑237 (MQ=255)

                                                                                                                                                                                                                  gcggcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGCACCCGGGTAAAGTTCCGGCGGTGGAACAGATCCTCACGGa                            >  1:60012/1‑238 (MQ=255)

                                                                                                                                                                                                                    ggcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACt                         >  1:766965/1‑239 (MQ=255)

                                                                                                                                                                                                                                tggcccgggggaGGTGTTTGCCGGCT‑GGCGCTCGTTGCC‑GGGCCACGGCAGACGGGTCAGGGCAGGTTCGGTTTCCGGATGCAGTAACTTCGCCTCGACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCa             <  2:1055892/228‑1 (MQ=255)

                                                                                                                                                                                                                                  gCCAGGGGGGGGTGTTTTCCGGGG‑GGCGCACGTTGCC‑TGGCCACGCCAGGCGGGGCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACt           <  2:214551/239‑1 (MQ=255)

                                                                                                                                                                                                                                  gCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACCCGGATAACGTTCAGGCGGTGGAAACGATCCTCACGGAACTTACCTTCCTGCAc            >  1:384149/1‑238 (MQ=255)

                                                                                                                                                                                                                                    cAGGGGCGGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCg         <  1:849654/239‑1 (MQ=255)

                                                                                                                                                                                                                                    cAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACt           <  1:13506/237‑1 (MQ=255)

                                                                                                                                                                                                                                      gCGGCAGGTGTTTGCCAGCT‑GGCCCACGTTGCC‑TGGCCACGCCAGACGGGTCAGAGCAGCTTCGGTTTCCGGATGCAGGAACTTCGCTTCTACGCCCAGGTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCt       <  1:213635/239‑1 (MQ=255)

                                                                                                                                                                                                                                       cGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTc          >  1:707673/1‑235 (MQ=255)

                                                                                                                                                                                                                                          cAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGtt    <  1:838222/238‑1 (MQ=255)

                                                                                                                                                                                                                                            ggTTTTTTCCAGCC‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCg  <  1:850140/238‑1 (MQ=255)

                                                                                                                                                                                                                                            ggTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑Ct                                                                                                                                   <  1:569750/110‑1 (MQ=255)

                                                                                                                                                                                                                                            ggTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑Ct                                                                                                                                   >  2:569750/1‑110 (MQ=255)

TGCGTATGTCGCAACGCGGTCGTCAGTAACGTCCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCT‑GGCGCACGTTGCC‑TGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAAC‑CTGTAAAAAATGGCGCGCCAGAC‑GGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCG  >  NC_000913/4053953‑4054426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`