Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,142,657	C→G	16.7%	G43G (GGC→GGG)	frwC →	putative enzyme IIC component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,142,657	0	C	G	16.7%	202.1 / 6.9	86	G43G (GGC→GGG)	frwC	putative enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base C (14/56);  new base G (14/2);  total (28/58)
Fisher's exact test for biased strand distribution p-value = 7.09e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCATCGTGGTGCATTACCTGAAGAAA  >  NC_000913/4142420‑4142894                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        aaaGTCCATTAAATGGCCTTTTTTTCCCCTGTTTGCTCCGCCTTGGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGgcg                                                                                                                                                                                                                                                        <  2:789675/239‑1 (MQ=255) aaaGTCCATTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGgcg                                                                                                                                                                                                                                                        <  2:835620/239‑1 (MQ=255)         tttaaTGGCTTTTTTTTCCCTTGTTTTCCCCCGCTGCGATTGCCTTTTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCg                                                                                                                                                                                                                                               <  2:271413/236‑1 (MQ=255)          tAAATGGCCTGGTTATCCACTGTTTGCTCCGGCTGCGATTGCCTATTGTTTCCTCCATCAAAAAGATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCgg                                                                                                                                                                                                                                              <  1:613787/239‑1 (MQ=255)           aattGGCCTTTTTTACCCTTGTTTGCCCCGCCTGCGATTGCCTATTGTTCCCCCCATCAAAAATATGGGCCTGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGa                                                                                                                                                                                                                                             <  2:76262/236‑1 (MQ=255)           aaaTGGCCTTTTTTTCCCTTTTTTGCTCCGCCTGCGTTTGCTTTTTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGa                                                                                                                                                                                                                                             <  2:465002/239‑1 (MQ=255)           aaaTGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCgg                                                                                                                                                                                                                                              <  1:881509/238‑1 (MQ=255)           aaaTGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCgg                                                                                                                                                                                                                                              <  1:556073/238‑1 (MQ=255)               ggCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATAGGGCGGGATTTTGCTGGCGGGTTTCGG‑TAATGTGGTA‑TGG‑AAAGGGGCacgggcgggaggc                                                                                                                                                                                                                                          >  2:505422/1‑227 (MQ=255)                        atccagttgttGCTCCGCCTGCGATTGCCTATTGTTTCCCCCATCAAAAATATGGACATTGGCTTTCGCAAGCCCGGTAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑t                                                                                                                                                                                                                             <  1:1054563/230‑1 (MQ=255)                             cTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTTCG‑TCATGTTGTA‑TGGCCAAGGGGCAGTG‑CCGGGTGGCGTA‑ACC‑GG‑AC‑C‑aaa                                                                                                                                                                                                                      >  1:640353/1‑239 (MQ=255)                             cTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGCTTTCG‑TCTTTTTTGA‑TGGGCAAGGGGCGGGG‑TCGGATGCCGTA‑GCC‑GG‑TC‑C‑caa                                                                                                                                                                                                                      >  1:477208/1‑239 (MQ=255)                               tttttccccGCCTGCGATTTCCTTTTTTTTCCTCCATAAAAAATTGGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATc                                                                                                                                                                                                                    <  2:541791/232‑1 (MQ=255)                               gTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAACAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CCTGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGGTTCCGCTCAGTTTGTT‑TGGAAATGCGGCAGTC‑CGGGATGCCGAA‑GCC‑GA‑CC‑C‑aaac                                                                                                                                                                                                                     >  2:762043/1‑238 (MQ=255)                                tttGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGGTTCCG‑TCCTGTTGTA‑TGGGAAAGGGGCCGTG‑CCGGATGCCGTA‑GCC‑GC‑AC‑C‑AAATC‑t                                                                                                                                                                                                                  >  1:138697/1‑239 (MQ=255)                                   tccccGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACGTTGGCTTTCGCAAGCCCGGGAACAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGaa                                                                                                                                                                                                               <  1:505422/236‑1 (MQ=255)                                        gCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTTCG‑TCACGTTGTA‑TGGCCAAGGCGCAGTG‑CCGGGTGCCGTA‑GCC‑GACCC‑C‑AAACT‑TGaa‑aaaa                                                                                                                                                                                                          >  1:50101/1‑239 (MQ=255)                                                   ccTTTTTTTTCCTCCATAAAAAATTGGGGCATGGGTTTTCGAAAGCCCGGGAGAAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAAGCCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATc                                                                                                                                                                                              <  2:374145/239‑1 (MQ=255)                                                   acTATTGGTTCCTCCATCAAAAATATGGACCTGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAAGGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGaa‑aaaa                                                                                                                                                                                                          <  1:509697/226‑1 (MQ=255)                                                          tttCCTCCATTAAAAATTTGGGCATGGGGTTTCGCAAACCCGGGCGCAGCTTATGAAGGAGTGGGTGCAGATCCTGAAAATCTCCCGTCAGCATTTAATGCCGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTg                                                                                                                                                                                       <  2:439345/239‑1 (MQ=255)                                                           ttCCTCCATAAAAAATATGGCAATGGGCTTTGGCAAGCCCGGGAGCACGTTTTGAATGAGTTGGGGCAGACCCTGAAAAATCCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGc                                                                                                                                                                                      <  2:82404/239‑1 (MQ=255)                                                            tcctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGGTTTCGG‑CCAT‑TTTTA‑TGGCACAAGGGGAGTG‑GCGGGTGCCCGT‑GGC‑GA‑TC‑C‑CAATA‑TTAA‑AAAAAAGtttttaagatggggtggg                                                                                                                                                                                     >  2:1050847/1‑224 (MQ=255)                                                            tcctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCC‑GCCTTTTGTA‑TGGCTAAGGGGCAGTG‑CCGGGTGCCGTA‑GCC‑GA‑TC‑C‑AAATCTTAAA‑AAAACTTTTTGattccgggtgtcg                                                                                                                                                                                      >  1:411405/1‑228 (MQ=255)                                                            tcctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGAATCGGGCGGTATTTTGCTGGC‑GGTTTTCT‑CTCTGTTTTAGTGGAAAAGCGGCGGGG‑CCGG‑TGCCGTG‑GCC‑GC‑TCAC‑AAATT‑TGAA‑AAAAATGttcaatttggggggtgg                                                                                                                                                                                      >  2:116076/1‑224 (MQ=255)                                                             cctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTTCG‑TCCTTTTTTT‑TGGGCAAGGCTGAGGT‑GCGGATGGCGTA‑ACCGGG‑CC‑C‑AAACT‑TGAA‑AAAATTTTTTTATTTCgcgtttgag                                                                                                                                                                                     >  1:885533/1‑231 (MQ=255)                                                                 cATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATTTTGTT‑TGGCAAAGGGGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCtt                                                                                                                                                                                >  1:340330/1‑239 (MQ=255)                                                                    cAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTAGTTTGCTGGC‑GGTTTACG‑TCATGTTGGT‑TGGAAAAGGGGCAGTG‑CCGGAGGGCGCA‑GGC‑GA‑TC‑C‑AAACA‑TGTA‑AAAACCATTTGATATCGGAGTTGGGGGCGTGAc                                                                                                                                                                             >  1:505302/1‑239 (MQ=255)                                                                     aaaaaTTTGGGCATTGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGATTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACg                                                                                                                                                                            <  2:1081847/239‑1 (MQ=255)                                                                                    ggCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTTCG‑TCCTTGTTTT‑TGGCAAAGGGGCAGGG‑CCGGGGGCCCGT‑GCC‑GG‑TC‑CAAAATC‑GGAA‑AAAACT‑TTTGTTAACGGCGGTGggggggttagcgggggtggggcctt                                                                                                                                                              >  2:824273/1‑217 (MQ=255)                                                                                      ttttCGAAAGCCGGGGAGCTGCTTTTGAATGAGTTGGTTCAGATCCTGAAAAATTCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGGGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTcc                                                                                                                                                            <  2:796201/237‑1 (MQ=255)                                                                                        ttCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCCTGTTGTT‑TGGCAAAGGCGCCGGG‑CCGGATGCCGTA‑GCC‑GG‑TC‑C‑ACATC‑TTCA‑AAAAATGTTTGGTATCTGGGTTTCGGGGtttgcctgggtggggccttttcctc                                                                                                                                                         >  1:1019140/1‑216 (MQ=255)                                                                                        ttCCCAAGCCCGGGGGCAGCTTATGAATGAGTTGGTGCAGTTCCTGAAAAATCCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCg                                                                                                                                                         <  2:477208/239‑1 (MQ=255)                                                                                         tCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTTCG‑GCCTGTTGTA‑TGGCAAAGGGGCCGTG‑CCCGGTGCCGTACGCC‑G‑‑TC‑C‑ACATC‑TGAA‑AAAACTTGTTGTTATCGGGGTTTCGGGGTTTGCGCTGATGGGGCCTTTtctccc                                                                                                                                                        >  1:139202/1‑237 (MQ=255)                                                                                          gcaaaGCCCGGGAGCAGGTTTGGAATGGGTTGGTGCCGGTCCTGAAAAATTCCCGTCAGCATTTAATGCCGGGCGTTGCACA‑CATGATTCCCTTCGTGGTTTCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCa                                                                                                                                                       <  2:623649/236‑1 (MQ=255)                                                                                                 ccggggggtgtttattaATGGGTTGGTGCAGATCCTGAAAAATCCCCGTCAGCATTTAAGGACGGGCTTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACa                                                                                                                                               <  2:546236/228‑1 (MQ=255)                                                                                                 ccgggacccttttttgattggGTTGGTTCTGTTCCTGAAAATTACCCTGATGCATTGAATGCCGGGCGTTTCACA‑CATTATTCCCTTCGTGGTGTCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACa                                                                                                                                               <  2:485920/225‑1 (MQ=255)                                                                                                 ccGGGAGCAGTTTTTGAATGGGTTGGTGCAGATCCTGAAAAATCCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACa                                                                                                                                               <  2:340330/239‑1 (MQ=255)                                                                                                 ccGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAGTACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CTTGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACa                                                                                                                                               <  1:76984/239‑1 (MQ=255)                                                                                                 ccGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATTCCCGTCAGCATTTAATGGCGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACa                                                                                                                                               <  1:133546/239‑1 (MQ=255)                                                                                                       gcagttttttaattggTTGGGCCAGTTCCTGAAAAATGCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGt                                                                                                                                          <  2:885533/223‑1 (MQ=255)                                                                                                       gcagtTTTTGAATGAGTTGGTGCAGATCCTGAAAAATCCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGt                                                                                                                                          <  2:1073446/238‑1 (MQ=255)                                                                                                       gcagtTTATGAATGAGTTTGTTCAGATCCTTAAAAATTCCCGTCCGCATTTAAGGGCGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATTTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGt                                                                                                                                          <  2:505302/238‑1 (MQ=255)                                                                                                       gaaggttATGAATGAGTTGTGGCAGTTCCGGAAAAATGCCCGGCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGt                                                                                                                                          <  1:1050847/233‑1 (MQ=255)                                                                                                            ttttgaatgaGTTGGTGCAGATCCTGAAAAATCCCCGTCAACATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTc                                                                                                                                     <  2:681870/235‑1 (MQ=255)                                                                                                            ttATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTAtt                                                                                                                                      <  1:824273/237‑1 (MQ=255)                                                                                                             tATTAATGGGTTGGGTCAGAACCTAAAAAATTCCCGTCAGGATTTAATGACGGGCGTTTCCCA‑CATGATTCCCTTCGGGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCa                                                                                                                                   <  2:640353/239‑1 (MQ=255)                                                                                                             tATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCa                                                                                                                                   <  1:33867/239‑1 (MQ=255)                                                                                                                 aatgaGTTGGTGCAGATCCTGAAAAATCCCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGc                                                                                                                                                                                      <  1:243707/185‑1 (MQ=255)                                                                                                                    ggtgtggtggCGATCCTGAAAAATTCCCGGCAGCCTTTAAGGGCCGGCGTTTCCCC‑ACGGATTCCCGTCGCGGTATCGGGCGGTTGTTTGCTGGC‑GGTTGCCG‑TCAGGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCaga                                                                                                                            <  2:689553/235‑1 (MQ=255)                                                                                                                     aGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGGG‑CCGGGTGCCGTA‑GCC‑GA‑TA‑C‑AAATC‑TGCA‑AAAACTGTTTGTTATCGGCGTTGCGGGCTTTGCGCTGCTGGTGCCCTTCCTCGCCGC‑GTACATCGGTTATTCCCTTTCcgag                                                                                                                           >  1:72655/1‑239 (MQ=255)                                                                                                                      gTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGGGCAGGG‑CCGGGGGCCGGA‑GCC‑GG‑TC‑C‑CAAAC‑TGAA‑AAAACTGTTTGATATCCGCGTTGCGGGCTTGTGGCTGATGGTGCCTTTCCTCCCCGC‑GGACATCGGTGATTTCAtttctaggc                                                                                                                          >  1:321852/1‑232 (MQ=255)                                                                                                                                    tGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGATTTCG‑TCATGTTTTA‑TTGGCAAGGCGCACTGCCCGGAT‑CCCTA‑GGC‑GA‑CC‑C‑AAATA‑TGAACAAAACTGTTTG‑TAGTGGCGTTTCGGGGTTTTCGGCGCTGGGGCCCTTCTTCCCACC‑GTACCACGGGTATTCCCTTTCCGAACGttttcggccggc                                                                                                             >  2:688821/1‑228 (MQ=255)                                                                                                                                       aaatttcccctccgtttttAATGACGGGCGTTTCACA‑ACTGATTCCCTTGGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGGG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                                                                                                         <  2:349330/223‑1 (MQ=255)                                                                                                                                       aaaaTTCCCGTCAGCATTTAATGGCGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                                                                                                         <  1:881254/239‑1 (MQ=255)                                                                                                                                                cgttcggatttaattgggggGTTTTACATCATGATTTCTGTCGGGGTATCGGGCGGGATTTGGGTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCGGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCGCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                                                                                                 <  2:513234/223‑1 (MQ=255)                                                                                                                                                       tttAATGGCGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCt                                                                                         <  1:204918/239‑1 (MQ=255)                                                                                                                                                       tttAATGCCGGGGTTTTCACA‑CAGTTTTCCTTTGGTGGTATCGGGCGGTATTTGGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCt                                                                                         <  2:1019140/239‑1 (MQ=255)                                                                                                                                                                gggCTTTGCACA‑CATGATTCCCTTCGTGGTATCGGGCTGTATTTTGCTGGC‑GGTTTCCG‑GCATGGTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTGCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGt                                                                                <  1:903247/238‑1 (MQ=255)                                                                                                                                                                  gctttttcca‑caTTTTTCCCTTCGTGGTATCGGGCGGTTTTTTGCTGGC‑GGTTTCCG‑TCATGTTTGA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTaa                                                                              <  2:138697/230‑1 (MQ=255)                                                                                                                                                                  gCGTTTCCCA‑CATGTTCCCCGTCGTGTGATGGGGTGGTTTTTGTCTGGC‑GGGTTCGT‑TCATGTTGTT‑AGGCACATGCGAAGTG‑CCGGATTCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGTTGCCGTGCCTGGCAGC‑GTACAGCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTaa                                                                              <  2:968973/238‑1 (MQ=255)                                                                                                                                                                              tggATTCCCGTCGGGGTACCGGTCGGTATTGTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATAGCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTg                                                                   <  1:326070/236‑1 (MQ=255)                                                                                                                                                                              aTGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGcc                                                                                                                                                                 <  1:331435/146‑1 (MQ=255)                                                                                                                                                                               tttttCCCTTCGTGGTTTCGGGCGGTTTTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGTAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCg                                                                 <  2:139202/236‑1 (MQ=255)                                                                                                                                                                               tgtttCCCTTCGTGGTTTCGGGGGTTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCg                                                                 <  2:321852/236‑1 (MQ=255)                                                                                                                                                                                gATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TTGCAAAGGCGCAGTG‑CCCGAAGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCTCAGG‑GTACATCGGGTATTCCCTTGCAGAACGTTCTGCGCTGGCTCCCTGCGCTATCGGGGCCT‑GGGTTGGGAACAGCTttgttggtg                                                                >  1:237210/1‑236 (MQ=255)                                                                                                                                                                                   tCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCaga                                                                                                                            <  1:348654/177‑1 (MQ=255)                                                                                                                                                                                    cccTGCGGGGTATCGGGCGGTAGTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGctt                                                            <  1:366082/239‑1 (MQ=255)                                                                                                                                                                                      cTTGGTGGTTGCGGGGGGTTTTTTGGTGGC‑GGTTTCCG‑TCAGGTGGTA‑TGGCAAAGGCGCAGGG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGcttct                                                          <  2:72655/239‑1 (MQ=255)                                                                                                                                                                                       ttCGTGGTTTCGGGGGTTTTTTTGCGGGC‑GGTTTCCG‑TCATTTTGTA‑GGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGcttctt                                                         <  2:493954/239‑1 (MQ=255)                                                                                                                                                                                       ttCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑GCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GT‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGcttctt                                                         <  1:417592/239‑1 (MQ=255)                                                                                                                                                                                        tCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATTTTTTA‑TGGGAAAGGGGCGGGG‑CCGGATGCCGTA‑GCC‑GC‑GC‑C‑AAAAC‑TTAA‑AAAACTGGTTTTTAACCGGGTTTCGGGGTTGGCGCCGATGGTGCCTTTCCTCCCCGCGGCACCCCGGGATTTCCTTTGC‑GCGCGTTCTGCTGTGGGTCCCTGCGCTATTGGGGGCTCGGGGGGGGAACAACTTTGGGGGGGGgttttt                                                         >  2:937579/1‑239 (MQ=255)                                                                                                                                                                                                tCGGGCGGTATTTTGCTGGC‑GGTTTCCC‑TCATGTTTTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGGA‑GCC‑CA‑TC‑C‑CAATC‑TGAA‑AAAAACGTTTGGTATCGGCGGTGCGGGCTTGACCCGGATGGGGCCTTTCCTCGCGAC‑GTAACTCGGGTGTTTCCATGCCGAGGGTTTTTCGCTGGCTCCGCGGGCTCTCGGGGCCC‑GGGTGGGTAAAAGCGTTGGTGCGGGGTGCTTTggggg                                                   >  2:264895/1‑233 (MQ=255)                                                                                                                                                                                                  gggCCGTATTTTGCTGGC‑GTTTTCCG‑TCATGTTGTA‑AGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAGTC‑TTGA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGa                                              <  1:535693/239‑1 (MQ=255)                                                                                                                                                                                                        tATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCa                                                                                                                              <  1:597190/154‑1 (MQ=255)                                                                                                                                                                                                                   c‑GGTTTCCG‑TTATGTTGTA‑TGGCAAAGGCGCAGGC‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATcgg                              <  1:1040138/238‑1 (MQ=255)                                                                                                                                                                                                                    gggTTTCCG‑TCTTTTTGTT‑TGGAAAAGGGGCCGTG‑CCGGATGCCGGA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATcgg                              <  2:483344/237‑1 (MQ=255)                                                                                                                                                                                                                    cgttttCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTTTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATcgg                              <  1:916674/235‑1 (MQ=255)                                                                                                                                                                                                                        ttCCG‑TCATGTTGTA‑TGGCAAAGGGGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCa                         <  1:883930/239‑1 (MQ=255)                                                                                                                                                                                                                        ttCCG‑TCATGTTGGA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GAC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTTTTTGATATCGGCGTTGCGGGCTTGCCGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCa                         <  1:721426/239‑1 (MQ=255)                                                                                                                                                                                                                           cG‑TCTTGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTG‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATTTCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCATCg                      <  2:942671/239‑1 (MQ=255)                                                                                                                                                                                                                               tcttttgttagggaaagggCGCGGTT‑CCGGAGGCCGTG‑GCC‑GA‑AC‑C‑AAATC‑GTAA‑AAACCTGTTGGTTGTCGGCGGTGCGGGCTTGCCGCTGATGGTGCCTTTCCTGGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGGGCGCTAGCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCATCgtc                    <  2:696423/222‑2 (MQ=255)                                                                                                                                                                                                                                      tA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCATCGTGGTGCAtt             >  1:785831/1‑238 (MQ=255)                                                                                                                                                                                                                                         tGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTTTCGGCGGCACCGGGGGGCATTccc          >  2:1046209/1‑239 (MQ=255)                                                                                                                                                                                                                                            cAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATGGGTGCCT‑GGGTTGGTAAAAGATTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCATCGTGGGGGATTACCTga       >  1:506006/1‑239 (MQ=255)                                                                                                                                                                                                                                              aaGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCCCTCGTCGCCGGGATTATCGGCGGCCTCGTGGGGCCTTACCCgaag     >  2:884345/1‑239 (MQ=255)                                                                                                                                                                                                                                               aGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTTGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCCGGCTTCTTTGGTGCACGTTTCGCCGGGTTTATCGGCGGCTTCGTGGTGCTATTCCTgaaaa    >  2:159010/1‑237 (MQ=255)                                                                                                                                                                                                                                               aGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGGATCGGGGGGCATAACCGgaaga    >  2:379034/1‑239 (MQ=255)                                                                                                                                                                                                                                               aGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGAAAACGCTTTGGTGGGGGCTTCTTTGGTGCCCTGATCGCCGGGATTATCGGGGGCATCGGGGGGCATTACCTgagga    >  2:1052438/1‑239 (MQ=255)                                                                                                                                                                                                                                                 gcgcAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCATCGTGGTGCATTACCTGAAGaaa  >  1:748340/1‑239 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        AAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACA‑CATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGC‑GGTTTCCG‑TCATGTTGTA‑TGGCAAAGGCGCAGTG‑CCGGATGCCGTA‑GCC‑GA‑TC‑C‑AAATC‑TGAA‑AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGC‑GTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT‑GGGTTGGTAACAGCTTTGGTGCGGGCTTCTTTGGTGCACTGATCGCCGGGATTATCGGCGGCATCGTGGTGCATTACCTGAAGAAA  >  NC_000913/4142420‑4142894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑