BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,156,549	T→C	13.0%	S180P (TCC→CCC)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,549	0	T	C	13.0%	287.7 / 3.0	111	S180P (TCC→CCC)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand): ref base T (61/30); new base C (4/13); total (65/46)
Fisher's exact test for biased strand distribution p-value = 1.10e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

AGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAG  >  NC_000913/4156311‑4156783

ggggttggttCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGctct                                                                                                                                                                                                                                               <  1:207976/235‑1 (MQ=255)

     tttttttgggtggtgggtataGCGTCAAAGTGCCCCAGGTTGATGAAGATTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GAc                                                                                                                                                                                                                                         <  2:353856/219‑1 (MQ=255)

            cggtgcgcgggACAGCGTCAAAGTGCCCCAGCTTGATGAAGAGTTAGGTCATGTTGGGCTGGCGCAGGCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑g                                                                                                                                                                                                                                 <  2:915622/229‑1 (MQ=255)

            cgggggcggggccAGCGTAAAAGGGACCCAGTTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑g                                                                                                                                                                                                                                 <  2:1004600/227‑1 (MQ=255)

            cGGTGACGGGGGCAGCGTCAAAGTGGCCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑g                                                                                                                                                                                                                                 <  1:605810/239‑1 (MQ=255)

            cGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGACACGCTGGGCGCGGATCTGATTTTGCTCTCC‑Gaggccgacg                                                                                                                                                                                                                                  >  1:283974/1‑231 (MQ=255)

             ggTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTGGCTTTCC‑GACGTCAGC‑g                                                                                                                                                                                                                                 >  1:1002500/1‑238 (MQ=255)

                 aCGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCCATGCCGACCAGGCGGCAACGGCCATGGCGGCACCGCCGGGCGCGGACCTGATTTTGCTCCCC‑GCCGCCACC‑GGcct                                                                                                                                                                                                                             >  2:333566/1‑236 (MQ=255)

                       aCAGCCTCAAAGGGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGGAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGa                                                                                                                                                                                                                       <  1:863238/238‑1 (MQ=255)

                           cGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCg                                                                                                                                                                                                                        <  1:167743/233‑1 (MQ=255)

                                    gccccAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGAAGCAAGGTTCCCCTAATCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGAAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCa                                                                                                                                                                                                           <  1:980443/235‑1 (MQ=255)

                                     aCCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGCAGGGCAACTGATGAACGTCAATGCAGACCAGGCGGCAACGGCGCTGGAGGCAACGCTGGGCGCGGTCCTAGTTTTGTCCTCC‑CACGTCCGC‑GGCAGTCCCGCCGGCAAAGGGctgg                                                                                                                                                                                                         >  2:167743/1‑235 (MQ=255)

                                      cccAGCTTGATGAAGAGTTAGGGCATGGTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACGCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCAGTCTCGACGGCAAAGGGCAACg                                                                                                                                                                                                        <  1:630827/238‑1 (MQ=255)

                                           cTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTg                                                                                                                                                                                                   >  1:1076382/1‑238 (MQ=255)

                                                tgaAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑AGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGCCAGC‑GCCATTCCCGACGCCCAAGGGCAACGCCTTGCCCaaa                                                                                                                                                                                             >  2:417080/1‑239 (MQ=255)

                                                     agTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCCTTGCCGAAATGAc                                                                                                                                                                                         >  1:303023/1‑238 (MQ=255)

                                                     agTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAc                                                                                                                                                                                         >  1:355900/1‑238 (MQ=255)

                                                     agTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGAAGGCAAAGGGCAACGAATTGCCGAAATGAc                                                                                                                                                                                         >  1:413197/1‑238 (MQ=255)

                                                         aGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAc‑cgccg                                                                                                                                                                                   >  1:105032/1‑239 (MQ=255)

                                                         aGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACCCATTCCCGAAATGAc‑ggccg                                                                                                                                                                                   >  1:381056/1‑239 (MQ=255)

                                                             cttgtgGGGCTGGCGCAGCCAGGTTCCCATAAGCTTATCAACTCCTTGCGGGAAAAC‑GGTTAACTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGaa                                                                                                                                                                               <  1:216512/237‑1 (MQ=255)

                                                             catttgggACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACCCCTTGCTGGAGAAC‑GGTTATCGGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGaa                                                                                                                                                                               <  1:110536/233‑1 (MQ=255)

                                                               tGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTTTCGCCGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGaaa                                                                                                                                                                              >  1:545363/1‑238 (MQ=255)

                                                               tGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCACC‑GGCATTCTCGACGGCAAAGGGAACGGCATTGCCGAAAAAAC‑CGCCGCGAAAg                                                                                                                                                                             >  2:521942/1‑239 (MQ=255)

                                                               tGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACCCTGGGCGCGGATCTGATTTTGCTCTCC‑GCCGTCAGC‑GGCAATCTCGCCGGCCAAGGGCAACGCATTTCCGAAAtaaacccccgcaaag                                                                                                                                                                              >  1:990357/1‑225 (MQ=255)

                                                                gTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGc                                                                                                                                                                            >  1:266221/1‑239 (MQ=255)

                                                                 ttGGCCTGGCGCCCCCGGTTTCCCTTAGTCTTTTCAACCCCTTGCTGGAGAAG‑GGTTATCTCCGGGTGGTCCGCTCCATGGGGGTAAAAGACGAAGGGCAACTGAAGAACGTCAATGCCGACCAGGCGGCAAGGGCGCTGGCGGAAACGCTGGGCGCGGATCTGATTTTGCTCCCC‑GACGTCAGC‑GGCATTCTCGACGGAAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCa                                                                                                                                                                           <  2:1002500/239‑1 (MQ=255)

                                                                  ttgccTGGCGCAGCCGTGTTCGCTGAATTTTACCATCTCCTTGCGGGAGAAG‑TGTTTCCTGCCGGTGGTCCGCCCCATGGGGGTAACAGCCGAAGGGAAACTGAGTACCGTCAATGCCGGCCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCCCCCC‑GCCGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAg                                                                                                                                                                          <  2:283974/235‑1 (MQ=255)

                                                                    gACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGTAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGac                                                                                                                                                                        >  1:807430/1‑238 (MQ=255)

                                                                       tGGCGCAGCCAGGTTCGCCTAGGCTTAACAACGCCGTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGaaca                                                                                                                                                                      <  1:186877/238‑1 (MQ=255)

                                                                         gcgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACATCAGC‑GGCATTCCCGACGGCAAAGGGCAACGCATTGCCGAAAGGAC‑CGCCGCGAAAGCAGACCACCt                                                                                                                                                                   >  1:698225/1‑239 (MQ=255)

                                                                          cgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCCCC‑GCCGTCAGC‑GGCATTCTCGCCGTCAAAGGGGAACGCATTTCCGAAATTAC‑CCCCCCCAAAACAaaaaaaaca                                                                                                                                                                  >  2:1045105/1‑236 (MQ=255)

                                                                          cgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGATCTCC‑GCCGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAGAGCAGaaaaacgg                                                                                                                                                                  >  1:1083682/1‑237 (MQ=255)

                                                                          cgcAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACCCTGGGCGCGGATCTGATTTTGCTCTCC‑GCCGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCCTTGCCGCAATGAC‑CGCCGCGACAGCAGaaca                                                                                                                                                                      >  1:1027184/1‑235 (MQ=255)

                                                                               ccAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGCCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGCCAAAGGGCAACGCCTTGCCGAAATAAC‑CCCCCCGAACCCCGAAAAACGGATTGa                                                                                                                                                             >  2:933732/1‑239 (MQ=255)

                                                                                cggtttCGCCTGAGCTTATCAATCCGTTGTGGGAGAAG‑GGTTCGCTGCCGGTGGGCGGCTCCTTGGGCGTAACAGCAGAGGGGATACTGGTGAACGTCAATGCCGACCGGCGGGCAAGGGGGCTGGCGGAAACGCTGGGGGCGGATCTGATTTTGCTCCCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCACCGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAg                                                                                                                                                            <  2:545363/235‑1 (MQ=255)

                                                                                catgTTCGCTTATGCTTTCCACCCCCGTGC‑GGAGAACGGGTTTCCTGCGGGTGGCAAGCCCCATTGGGGTAACGGACGAAGGGCAACTGAGGAACGTAAATGCCGACCGGGCGGCAACGGGGCTGGGGGCAACGCTGGGCGCGGATCTGATTTTGCCCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGAAACGAATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAg                                                                                                                                                            <  2:315135/236‑1 (MQ=255)

                                                                                   aaaagggacagctttcaaaCTCCTGGCTGGAGAAG‑GGTTATCTGCGGGTGGTCAGCTCCAGTGGGGAAACAGAGGAAGGGCACCTGATGAACGTCAAGGCCGACCAGGCGGCAACGGCGCGGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAg                                                                                                                                                         <  2:891199/222‑1 (MQ=255)

                                                                                    ttCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAg                                                                                                                                                         <  1:225895/238‑1 (MQ=255)

                                                                                    tctgtcTATGCTTATCAACTCCTGTGTGGAGAAC‑GGTTATCGGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCACCGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAg                                                                                                                                                         <  2:228017/233‑1 (MQ=255)

                                                                                                 accAACGCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCGCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGa                                                                                                                                           <  1:698447/237‑1 (MQ=255)

                                                                                                   caccTCCTTGCGGGAGAAC‑GGTTATCGGCCGGTGGTCAGCTCAATGGGCGTAACAGACGAAGGGCAACGGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGAAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACg                                                                                                                                         <  1:194484/236‑1 (MQ=255)

                                                                                                       tCCTTTTTGGAAACC‑GGTTTTCTGCGGGTGGTCCGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCAt                                                                                                                                     <  2:381056/239‑1 (MQ=255)

                                                                                                       tCCTTGGTGGGAAAC‑GTTTATCGGCCGGTGGCCAGCCCCTTGGGCGTAACAGCCGAGGGGCAACGGATGAACGGAAATGCCGACCGGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCCCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCAt                                                                                                                                     <  2:799390/239‑1 (MQ=255)

                                                                                                       cccttgtggggaaaC‑GGTTTTCTGCGGGGGGCCGGCCCCCTGGGGGTAACAGAGGAGGGGAAACTGAGGAACGGCAAGGCCGCCCGGGGGGCAACGGGGCTGGCGGCAACGCGGGGCGGGGATCTGATTTTGCTCCCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCAt                                                                                                                                     <  2:303023/227‑1 (MQ=255)

                                                                                                        ccTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATg                                                                                                                                    >  1:799390/1‑239 (MQ=255)

                                                                                                        ccTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGCCATg                                                                                                                                    >  1:288148/1‑239 (MQ=255)

                                                                                                        ccTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGACCTGATTTTGCTCCCC‑GCCGTCAGC‑GGCATTCTCGCCGGCAAAGGGCAAGGACTTGCCGAAATGCC‑GGCCGCGCAAGCAGAAACAAGGACTGAGAAGGGCAATATAACTAACggaagg                                                                                                                                    >  2:473348/1‑235 (MQ=255)

                                                                                                         tttGCGGGAGACC‑GTTTTTCGTCCGGTGGCGACCCCAATGGGAGGAACAGACGAGGGGCAACGGATTAACGTCAAGGCCGACCAGGCGGAAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTTATTTTGCTCGCC‑GACGTCAGC‑GGCATTCTCGACGTCAAAGGGCATCGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACt                                                                                                                                             <  2:698740/228‑1 (MQ=255)

                                                                                                         cTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGCCGGCAAAGGGCAACGCATTGCCGAAATGAC‑CCCCCCGAAAGCAGAACAACTGATTAGGAAGGGCATAATTATTAACGCCagag                                                                                                                                   >  2:757471/1‑236 (MQ=255)

                                                                                                         cTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCa                                                                                                                                                                                                                  >  1:565606/1‑161 (MQ=255)

                                                                                                         cTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCa                                                                                                                                                                                                                  <  2:565606/161‑1 (MQ=255)

                                                                                                         cTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCAttatta                                                                                                                                               >  1:891199/1‑227 (MQ=255)

                                                                                                         cTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCCGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAAATGATTGAGCAGGGCATTATTACTGACGGGATGa                                                                                                                                   >  1:292816/1‑239 (MQ=255)

                                                                                                           tGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGCTTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCCCAATGAC‑CGCCGCGCAACCGGACAAACTGATTAAGCAGGGCATTTTTACTGCCGGCCAGATa                                                                                                                                 >  2:442414/1‑239 (MQ=255)

                                                                                                           tGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGaaa                                                                                                                                 >  1:1079859/1‑237 (MQ=255)

                                                                                                           tGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CCCCGCGAAAGCAGAACAACGGATTGAGAAGGGCATTATTACTGACGGCAAGaaa                                                                                                                                 >  2:599366/1‑237 (MQ=255)

                                                                                                                   aaC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGCTGAACGTCAATGCCGCCCAGGCGGCAACGGCGCTGTCGGCAACGCTGGGCGCGGATCTGCTCTTGGTCTCC‑GACGCCAGC‑GGCATTCTCGCATGCACAGGGCAACGACGTGCCGCAA‑GACGCGCCGCGAAGGCAGGACCACTGCTTTACCCGGGCcttattcatggccgactgataatagaagg                                                                                                                         >  2:769447/1‑216 (MQ=255)

                                                                                                                   aaC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCAGGa                                                                                                                                   >  1:698740/1‑229 (MQ=255)

                                                                                                                           tttgcccGGGGGTCAGCCCCATGGGCGAAAAAGGCAAAGGGCAAGCGATGAACGGCAATGCCGACCGGGCGGCAACGGCGGGGGCGGCAACGCGGGGCGCGGATCTGATTTTGCTCCCC‑GACGTCAGC‑GGAATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAAcgcg                                                                                                                  <  2:1079859/234‑1 (MQ=255)

                                                                                                                           aTCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGCTCTGATTTTGCTCTCC‑GACGTCCGC‑GGCATTCTCGCCGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGAAGAACAACTGATTGAGCAGGGCATTATTACTTACGGCAAGGAAGTGAAAGGGAAagcg                                                                                                                  >  2:360818/1‑239 (MQ=255)

                                                                                                                                ccGGTGGTCAGCTCCACTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACgcggc                                                                                                                >  1:674807/1‑236 (MQ=255)

                                                                                                                                 cGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGACAGCAGAACAACTGATTGAGCCGGGCATTATTCATGACGGCCTGTAagggaaaaggaaaggggggcgg                                                                                                            >  2:878116/1‑224 (MQ=255)

                                                                                                                                           ccccATTGGGTGAACAGCCGAAGGGAAACTGATGAACCGCAAGGCCGACAGGGCGGCACGGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCCCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGccc                                                                                                  <  2:164131/237‑1 (MQ=255)

                                                                                                                                             ccATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTTACGGCATGAGagtgaaagtgaaacggggcggggggggggcccgc                                                                                                >  2:938904/1‑217 (MQ=255)

                                                                                                                                                ttGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATATTGAAAGGGAACGCGGCGCCGGAGGCGGCCCGccc                                                                                              >  1:341331/1‑236 (MQ=255)

                                                                                                                                                ttGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGccc                                                                                              >  1:972249/1‑236 (MQ=255)

                                                                                                                                                     gTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTCCTGACGGCATGAAAGGGAAAGGGAACGCGGCGCTGGAGGCGGCCCGCCCGCAggg                                                                                        >  2:711427/1‑239 (MQ=255)

                                                                                                                                                      tAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGAGAGTGAAAGGGAACGCGGCGCTGGAGGCGGCCCGccaccggggg                                                                                       >  2:522040/1‑230 (MQ=255)

                                                                                                                                                      tAACAGACGAAGGGCAACTGAGGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGGGGCAACGGTGGGCGCGGATTTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTggg                                                                                        <  1:333566/238‑1 (MQ=255)

                                                                                                                                                        aCAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGCTTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCg                                                                                     >  1:1010498/1‑239 (MQ=255)

                                                                                                                                                         cggagaaggggAAACGGATGAACGTAAATGCCGACCGGGCGGCAAGGGCGCTGGGGGCAACGCGGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGTAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGt                                                                                    <  2:807430/231‑1 (MQ=255)

                                                                                                                                                              gAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGCTTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCCAAGGGCAACGCATTGCCGCAATGAC‑CGCCGCGAAAGCAGAAAAACTGATTGGGCAGGGCATTCTTACTGCAGGCATGATAGTGAAAGTTAACGCGGCCCGGGATGGGGCCCGCACGCTGGGCCTCCCGGt                                                                               >  2:328809/1‑239 (MQ=255)

                                                                                                                                                              gAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGt                                                                               >  1:189764/1‑239 (MQ=255)

                                                                                                                                                                 gggCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGGGCAGGGCATTATTACTGACGGCATGATAGTGAAAGGGAAACCGGCGCTGGCTGCGGCCCCCACCCTGGGCCGTCCgggcgg                                                                            >  2:130740/1‑235 (MQ=255)

                                                                                                                                                                      aCTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCCGGGCATTATTACTGACGGCCTGAAAGGGAAAGGGAACGCGGCGCTGGGAGCGGCCCCCCCGCTGGGCCCGCCggggggtaac                                                                        >  2:626517/1‑230 (MQ=255)

                                                                                                                                                                      aCTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCCCCCTGGGCCGCCCGGTGGaattc                                                                        >  1:42233/1‑234 (MQ=255)

                                                                                                                                                                            gaACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGTTGCGGCCCGCACGCTGGGCCGTCCGGGAGATAACGcct                                                                    >  1:405398/1‑236 (MQ=255)

                                                                                                                                                                               cGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGCC‑CGCCGCTAAAGCAGGAAAACTGATTGAGCAGGGCATTATTACTGACGGCCTGCTCGTGAAAGTGACCGCGGCGCTGGATGCGGCCCGCCCCCTCGGCCGTCCGGGAGAGTTCGCCCCCTgcg                                                              >  2:760530/1‑237 (MQ=255)

                                                                                                                                                                                ctCAAGGCCCGCCCGGCGCCAACGGCGCTGGGGGAAACGCGGGGCGGGGATCTGATTTTGCCCCCC‑GACGTCGGC‑GGCATTCTCGACGGAAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCg                                                             <  2:387079/238‑1 (MQ=255)

                                                                                                                                                                                acgactccgacccggggggcAAGGGGGGTGGCGGAACCGGTGGGCGGGTGTCTGTTTTTGCCCACC‑GGCGT‑AGCGGGCATTCCCGAGGGCAAAGGGCACCGAGTGGCCGAAATGGC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCg                                                             <  2:135622/222‑1 (MQ=255)

                                                                                                                                                                                    aTGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGCCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCCGGGATATATTACCGCCGGCCTGATAGTGAACATGAACGGCGCGCTGGATTCGGCCCGACCGCGGGGCCGCCCGGCAggatacaccccccggcggaat                                                         >  2:1013779/1‑219 (MQ=255)

                                                                                                                                                                                          aCCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGAAATCGCCTCCTGGCGTCATGCGGa                                                    >  1:680532/1‑238 (MQ=255)

                                                                                                                                                                                            cAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAAGCCGGCGCTGGATGCGGCCCGCACACCGGGCCGGCCGGGAAATATCCCCCCCTGGCGCCaacgggggca                                                 >  2:830724/1‑230 (MQ=255)

                                                                                                                                                                                                      cggcggTGGCGGAACGGTTGGGGGGGGTTTTGTTTTTGCCCCCC‑GCCGGCAGC‑GGACTTCCCGACGGAAAAGGGCAACGCATGGCCGAAAGGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                       <  2:105032/239‑1 (MQ=255)

                                                                                                                                                                                                      cggcggGGGGGGCAACGGTGGGCGCGGATCTGATTTTGCCCCCC‑GGAGTAAGC‑GGCATTCCGGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                       <  1:711427/239‑1 (MQ=255)

                                                                                                                                                                                                      cGGCGCTGGCGGGAACGCGGGGCGGGGATCTGATTTTGCCTGCC‑GACGTCAGC‑GGCAGTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                       <  1:933732/239‑1 (MQ=255)

                                                                                                                                                                                                      cGGCGCTGGCGGCAACGCGGGGGGCGGATCTGATTTTGCCCTCC‑GAAGTCAGC‑GGAATTCGCGACGGCAAAGGGCACCGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                       <  1:830724/239‑1 (MQ=255)

                                                                                                                                                                                                      cGGCGCGGGCGGAAACGGTGGGCGGGGATTGGATTTTGCTCCCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                       <  1:599366/239‑1 (MQ=255)

                                                                                                                                                                                                            gggcggcAACGTGGGGCGGGGATCGTATTTTGC‑CTCCGGAACACAGG‑GGCAATCCCACCGCAAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGttt                                 <  1:440509/238‑1 (MQ=255)

                                                                                                                                                                                                               gggacagggtGGGCGGGGATTTGGTTTGCCCCCCC‑GCCGTCGGC‑GGCTTTCTGACCGAAAAAGGGGAAGCGATCGCCAAAATGAC‑CGCCGGGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                               <  2:189764/229‑1 (MQ=255)

                                                                                                                                                                                                               cggaAACGCTGGGCGGGGATCTGATTTTGCTCTCC‑GACGTGAGG‑GGCATTCCCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                               <  1:810980/238‑1 (MQ=255)

                                                                                                                                                                                                                   aaCGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGAGGCGGCCCGCACGCTGGGCCGTCCGGTAGCAATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTa                          >  1:1040773/1‑239 (MQ=255)

                                                                                                                                                                                                                        tGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTTTGCCg                     >  1:741775/1‑239 (MQ=255)

                                                                                                                                                                                                                                  ttttaTTTTGCTCCCC‑GACGTCAGC‑GGCATTCTCGACGGAAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTAcg            <  1:757471/234‑1 (MQ=255)

                                                                                                                                                                                                                                  tCTGATTTTGCCCTCC‑GACTTCAGC‑GCATTTCCAGACGGCAAAGGGCAACTCTTTGCCAAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTAcg            <  1:473348/238‑1 (MQ=255)

                                                                                                                                                                                                                                           gCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGGGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGGACGCGGATTTTa   >  1:425304/1‑238 (MQ=255)

                                                                                                                                                                                                                                           gCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTa   >  1:246201/1‑238 (MQ=255)

                                                                                                                                                                                                                                           gCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGAACGCGGATTTTAg  >  1:931867/1‑239 (MQ=255)

                                                                                                                                                                                                                                           gCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGACGCTGGATGCGGCCCGCACGCTGGGCGCTCCGGTAGATATAGCCCCCCGCCGGCAACCGGCCCCGCTTCCGCCCCTGTTTACAGGTAAGCCGAAGGGGAGGGGGAttgtgg  >  2:591574/1‑235 (MQ=255)

AGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGGCGCAGCCAGGTTCGCCTAAGCTTATCAACTCCTTGCTGGAGAAC‑GGTTATCTGCCGGTGGTCAGCTCCATTGGCGTAACAGACGAAGGGCAACTGATGAACGTCAATGCCGACCAGGCGGCAACGGCGCTGGCGGCAACGCTGGGCGCGGATCTGATTTTGCTCTCC‑GACGTCAGC‑GGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGAC‑CGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAG  >  NC_000913/4156311‑4156783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`