BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,993	T→G	17.5%	S87S (TCA→TCC)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,993	0	T	G	17.5%	270.4 / 6.5	125	S87S (TCA→TCC)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (62/35); new base G (4/22); total (66/59)
Fisher's exact test for biased strand distribution p-value = 1.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

AAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATA  >  NC_000913/4241757‑4242229

aaggagaCCAGTTCCCCGGAAATATGAGCTGGCGCCAGTTCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGa                                                                                                                                                                                                                                               <  1:190062/236‑1 (MQ=255)

      aacAGTTTCCCGCGAATATGAGCTGGAGCCACTTCCGCAATAT‑TCATTGGCGAGAGCACTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGCTCAATAACAAATTTCGGGACATAACCTACCAGAGTCATGGG‑CACACTGTTGTCC‑GGG‑ATTCT‑CGAGGG‑AAAACCCAGTTC‑AATCCAG‑GCAGAA‑CGTACA‑CGAGCGAGCGAACACAGGAA‑C‑GCAGCAATATTAATGGAATCacg                                                                                                                                                                                                                                         >  2:694977/3‑239 (MQ=255)

      aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCacg                                                                                                                                                                                                                                         >  1:598804/1‑239 (MQ=255)

             tCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CAAGAAATAAAAAACAGGAA‑A‑GCAGCAATCTTAAGGGAATCCCGACGACCg                                                                                                                                                                                                                                  >  1:1008141/1‑239 (MQ=255)

                 gcgAATATGAGCTGGAGCCAGTTCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑Gc                                                                                                                                                                                                                             >  1:879854/1‑239 (MQ=255)

                     atagGAGCGGGAGCCAG‑TCCCCAAAATAACATTGGCAAGATCATTGAGGCAAAACCACCCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGtt                                                                                                                                                                                                                         <  1:467956/239‑1 (MQ=255)

                      taggagatggacccggccccGAAATAT‑ACATGGGGGAAAGCATGGAGGATAAACCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTa                                                                                                                                                                                                                        <  1:954767/221‑1 (MQ=255)

                              ggAGCCAGTTCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACCGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATa                                                                                                                                                                                                                >  1:614012/1‑239 (MQ=255)

                              ggACCCAGTTCCGAAATAC‑ACTTTGGCGAGAGCATTGAGGCTAAACCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAAt                                                                                                                                                                                                                 <  1:31263/238‑1 (MQ=255)

                                acccAGTTCCGAAATAT‑ACATGGGCGAGAGCATTGAGCAAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATaa                                                                                                                                                                                                               <  1:581259/236‑1 (MQ=255)

                                      ttCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCCCC‑Ga                                                                                                                                                                                                       >  1:773148/1‑239 (MQ=255)

                                                      tGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGGTACTGCAATAACAACC‑GAGGGCACCGCCgatga                                                                                                                                                                                        >  1:987761/1‑239 (MQ=255)

                                                       ggCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAACTAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTAATGCAATAACCACC‑GAGGGCACCCCCgatgaa                                                                                                                                                                                       >  2:658585/1‑238 (MQ=255)

                                                         cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGCTGATGc                                                                                                                                                                                     >  1:511968/1‑239 (MQ=255)

                                                         cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑ACGGTTACTGCAATAACCACC‑GAGGGACACGCCgagaaggc                                                                                                                                                                                     >  1:584092/1‑233 (MQ=255)

                                                         cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCAAA‑GCGGTTCCTGCAATAACCACC‑GAGGGCACCGCCGATGATGc                                                                                                                                                                                     >  1:1028656/1‑239 (MQ=255)

                                                         cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACAACCGAA‑GCGGTAACTCCAATAACCACC‑GAGGGAAacgcccaggagga                                                                                                                                                                                     >  2:1082042/1‑231 (MQ=255)

                                                           agagatttGGGGCTAACCCAACCAAAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTTTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑GGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATACCCACC‑GAGGGCACCGCCGATGATGCa                                                                                                                                                                                    <  1:910416/232‑1 (MQ=255)

                                                           agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGGGAATCACGACAACCGAA‑GCGGTTACTGCAAAAACCACC‑AACGGCACCCCCgacacagaaa                                                                                                                                                                                   >  2:358730/1‑231 (MQ=255)

                                                             agCGTTGAGGAAAAACAAGCACAAAGACCGCAAATAATGCAATAAATAAAAAATTCGGGGAAATAACCTGCCAGATAAACAGG‑CCCAGTGTTGTCA‑GGG‑TTTAT‑AGGGGT‑AAAACCAATTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑TCGCAAATCTTAAGTGAATCACGACGACCGAA‑GCGTTTACTGCAATAACCACC‑GGGGGCACCGCCGATGATGCAAc                                                                                                                                                                                  <  1:886565/238‑1 (MQ=255)

                                                             agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAAcc                                                                                                                                                                                 >  1:525606/1‑239 (MQ=255)

                                                             agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAAcc                                                                                                                                                                                 >  1:334190/1‑239 (MQ=255)

                                                             agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATAACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAAcc                                                                                                                                                                                 >  1:212103/1‑239 (MQ=255)

                                                                  gtgaaaaaaacccccacaaaacccGACAATAAGGAAAAAAAAAAAAAATTCCGGAACATACCCTGCCAGATAAACAGG‑CACAGTGTTGT‑C‑GGG‑GTTAA‑AAAGGTAAAAACCAAGTTC‑GGCCCAG‑CAAGAC‑CCAACA‑CCAAAAAAAAAAAACAGGAC‑A‑GCACAAATCTAAAGTGAACCACGACGACCGAA‑GCGGTTACGGCAATACCCCCC‑GAGGGCACCGCCGATGATGCAACCAATCa                                                                                                                                                                            <  2:212103/217‑1 (MQ=255)

                                                                        tAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTCCAATAACCACC‑GAGGGCAACGCCGATGATGCAACCAATCAGAgccc                                                                                                                                                                      >  1:921165/1‑237 (MQ=255)

                                                                         aaaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGATTCACGACGACCGAA‑GCGGGTACTGCAAGAACCACC‑AAGGGCACCGCCGATGCGCCAACCAATCAGAgcgcg                                                                                                                                                                     >  1:496784/1‑238 (MQ=255)

                                                                          aaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTAACTGCAATAACCACC‑GAGGGACCCGCCGATGATGCAACCAATCAGAGCGCt                                                                                                                                                                     >  1:1079087/1‑238 (MQ=255)

                                                                            caacccacaaacccGCAAAGAATGCGATAAATAACAAATCCCGGGACATAACCTGCCAGATAACCAGG‑CAAAGTTTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAACCCAAGTTC‑TGGCCAG‑GCAGAC‑CCTACC‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGGGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGc                                                                                                                                                                  <  1:401106/227‑1 (MQ=255)

                                                                                cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTGACTGCAAAAACCCCC‑GAGGGCACCCCCGATGAGGAAACCAAACAGAGCACTGGCCACg                                                                                                                                                              >  2:347918/1‑239 (MQ=255)

                                                                                   acacccccccAATAATGAAAAAAAAAAAAAATCCGGGAAAAAAACCCGCGAAATAAACGGG‑CACAGTGTTGCCC‑GGG‑TTTAA‑AGGGGT‑AAACCCAAGTTC‑GGGCCAG‑GCAGAA‑CCTACA‑CCAGAAAAAAAAAAAAGGAC‑A‑GCAGCAATCTTAAGTGAATCCCGACGACCGAA‑GCGGTTACTACAAAAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                           <  2:635009/231‑1 (MQ=255)

                                                                                   aaaaccccgaaaatggtgaaaaaaaaaCAAATTCGGGGAAATACCCGCACAAATAAACGGG‑AACAGTTGTGCCC‑GGG‑TTAAA‑AGAGGA‑AAAACCAAGTTC‑TGGCAAG‑GCAGAA‑CCAACA‑CCAAAAAAAAAAAACAGGAC‑A‑GCAACAATGTTAAGTGAATCCCGACGCCCGAA‑GGGTTTACTGAAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                           <  2:655517/218‑1 (MQ=255)

                                                                                      tACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACGGCAATAACCACC‑GAGGGCACCGCCGATGAAGAAACCAAACaaaaccatggcccacacaaa                                                                                                                                                         >  2:183073/1‑219 (MQ=255)

                                                                                                  tGCGATAAATAACAAATTCCTGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTa                                                                                                                                                                                                                        <  2:889544/164‑1 (MQ=255)

                                                                                                  tGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTa                                                                                                                                                                                                                        >  1:889544/1‑164 (MQ=255)

                                                                                                   gcgcTAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGCAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCCCC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACCGGGAGt                                                                                                                                           >  1:161259/1‑239 (MQ=255)

                                                                                                   gCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGt                                                                                                                                           >  1:56962/1‑239 (MQ=255)

                                                                                                      ataaataaCAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACGCACAACCCTAACAGGGAGTTg                                                                                                                                         >  1:78479/1‑238 (MQ=255)

                                                                                                       aaaaaaaCAAATCCCGGGACAAAACCTGCCAGAAAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAA‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGCA‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGc                                                                                                                                       <  1:688057/238‑1 (MQ=255)

                                                                                                             aCAAATCCGGTGACATAACCTCCCAGAAAAACAGG‑CAAATTTTGTTCC‑GGG‑TTTAG‑AGAGGA‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAGCAGGAC‑A‑GCAGAAATCTTAAGTGAACCCAGAGGCCCGAA‑GGGGTTACTGCAATACCCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGa                                                                                                                                 <  2:1023294/239‑1 (MQ=255)

                                                                                                              aaaattccccgaattcagtcgCCAGATAAAAAGGACACGTGGTTTCCC‑GGG‑TTTAA‑AGAGGT‑AAACCAATGTTG‑CGTCCAG‑TCAATC‑TTTCCA‑CAAAAAATAAAAAACGAGGC‑A‑ACGACAAGCTAGAGTGAATCACGTCGTCTGAA‑GGGGATTGTACAATACCCGCC‑GGGGGCACCGCCGATGATGCAACCAATCAGAGAGCTGGCCACGCAAAAACCTAACAGGTAGTTGGCAGCGGa                                                                                                                                 <  2:728677/219‑1 (MQ=255)

                                                                                                                   tCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGATGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCAAGACGACCGAA‑ACGGTTACTGCAATAACCACC‑GAGAGCACCGCCGATGATGCAACAAATCAGCGCGCTGGCCACGCAAAACCCAAACAGGGAGTGGGCAACGGATGCACt                                                                                                                           >  2:763493/1‑239 (MQ=255)

                                                                                                                     cgtgACATAACCTGCCAGATAAACAGA‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACAAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCCGGCACAACCCTAACAGGGAGTTGGCAGCGGATTCACTTa                                                                                                                         >  1:880251/4‑239 (MQ=255)

                                                                                                                     cGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTa                                                                                                                         >  2:698661/1‑239 (MQ=255)

                                                                                                                      gggACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGTTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCCTTaa                                                                                                                        >  1:1023294/1‑239 (MQ=255)

                                                                                                                            tAACCTGACAGATAAACAGG‑CACAGTGTTGTCC‑GGT‑TTAAT‑AGAGGT‑AAACCCAAGTTC‑TGGCAAG‑GCAGAC‑CCTACA‑CCAAAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTtg                                                                                                                  <  1:590428/239‑1 (MQ=255)

                                                                                                                             aaCCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTACCTTAAGTTTtgt                                                                                                                 >  1:348131/1‑239 (MQ=255)

                                                                                                                                ccgcccagaaaaCAGGCCACAGTTTTGTCG‑GGG‑TTTAA‑AAAGGA‑AAAACCAAGTTC‑GGGCCAG‑GCAAAC‑CCAACC‑CCAGAAAAAAAAAACGGGAC‑A‑GCAGCAATCTAAAGGGAACCCCGACGACCGAA‑GCGGTTACGGCAATACCCCCC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTgg                                                                                                               <  2:525606/230‑1 (MQ=255)

                                                                                                                                 ctgctataaaaacacgctcgggtttgcCC‑GGG‑TTAAA‑AGAGGA‑AAAACCAGGCTG‑TGCCAAG‑GAAAAA‑CC‑ACACCCAAAAAAAAAAAACA‑GAC‑AGGCAGAACACTGAAGGGAATCACGACGCCAGAA‑GGGTTTACGGCAAAAACCACC‑GAGGGACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGa                                                                                                              <  2:334190/216‑1 (MQ=255)

                                                                                                                                  gCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCGGCGGATTCCCTTAAGTTTTTTGGAGc                                                                                                            >  1:914806/1‑239 (MQ=255)

                                                                                                                                  gCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGAGCTGGCCACGCAAAACCCCAACAGGGGGTTGGCCGAGGATTCCCTTAAGTTTTTTGGAga                                                                                                            >  2:85667/1‑238 (MQ=255)

                                                                                                                                         aaaacgggctagttgtgttCC‑GGG‑TTTAA‑AGGGGA‑AAAACCAAGTT‑‑TGGGCAGAGCAGAC‑CCTAAC‑ACAAAAAAAAAAAACGGGAA‑A‑GAAACACTCTAAAGTGAAACACGACGACCGAA‑GCGGTTACTGCAATACCCCCA‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaac                                                                                                         <  2:268162/218‑1 (MQ=255)

                                                                                                                                         aaaCAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGCGCAACAAAg                                                                                                     >  1:1002895/1‑239 (MQ=255)

                                                                                                                                          accggg‑CACAGTTTTGCCC‑GGT‑TTTAT‑AGGGGA‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CAAGAAATAAAAAACAGGAC‑A‑GCAGCCACCTTAAGGAAACCCCGACGACCGAA‑GCGGTTACTGCAATACCCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa                                                                                                    <  2:71041/235‑1 (MQ=255)

                                                                                                                                          aaacgg‑CACAGTGGTGGCC‑GGG‑TTTAA‑AGGGGT‑AAAACCAAGTTC‑GGGCCAG‑GCAAAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGGGAATCACGACGACGGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa                                                                                                    <  1:752826/235‑1 (MQ=255)

                                                                                                                                          aaaaggaccgggggttgcCG‑GGG‑TTTAA‑AGAAGA‑AAAACCAAGGTT‑GGGCCAG‑GAAACC‑CAAACC‑CAAAAAAAAAAAAACGGGAA‑A‑CCAAAAATGTAAAGGGAATCACAGAGACCAAA‑GCGGTTCCGACAAAAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAg                                                                                                     <  2:928574/226‑1 (MQ=255)

                                                                                                                                          aaCAGG‑GACCGGTTTTTCC‑GGGTTTTAA‑AGAGAT‑AAAACCAAGTTC‑GGGCAGG‑GCAAAA‑CCCACC‑CAAGAAAAAAAAAACAGGCC‑A‑GAAGAAACCTAAAGGAAATCCCGAAGACGGAA‑GGGGTTACT‑CAAAAACCCCCGGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAg                                                                                                     <  2:1028656/239‑1 (MQ=255)

                                                                                                                                           cgggg‑CACGGTGTTGTCC‑GGG‑TTTAA‑AGAGGT‑AAAACCAAGTTC‑GGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa                                                                                                    <  1:46764/235‑1 (MQ=255)

                                                                                                                                                     gtgtTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGATACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                                                                                          >  1:95857/1‑239 (MQ=255)

                                                                                                                                                       ttttttc‑GGG‑TTTTTAAGAGGA‑AAACCCAGTTTC‑GGGCCAG‑CCACAA‑CCTAAA‑CCAAAAAAAAAAAACGGGAC‑A‑ACACAAATCTAAAGGGAACCACGCCGACCGAA‑GCGGTTCCTCAAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt                                                                                         <  2:598804/233‑1 (MQ=255)

                                                                                                                                                       ttttgcagggg‑TTTAA‑AGAGG‑‑AAAACCCAGTTCTTGGGCAG‑CGAAAA‑CCTACC‑CCAGAAATAAAAAACAGGAA‑A‑ACAGAAATCCTAAGTGAATCACGACGACAGAC‑GCGTTTACTGAAATACCCACC‑GGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                                                                                          <  2:710634/230‑1 (MQ=255)

                                                                                                                                                        gtttacaggg‑TTGAT‑AGAGGT‑AAACCCAATGTC‑AGACCGG‑GGAGAA‑CCAACA‑CAAAAAATAAAAAACGGGAC‑A‑GAAGCAATCTTAAGTTAATCACGACTGCCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                                                                                          <  1:79937/230‑1 (MQ=255)

                                                                                                                                                         tttccgggg‑TTTAA‑AGAGG‑‑AAAACACAGTTT‑GGGCCAGAACAAAA‑ACAACC‑CCAAAAAAAAAAAACAGGAC‑G‑GCAGCAATCTTAAGTGACACCCCCCGACCGAA‑GGGGTTCCTGCAAAAACCCCC‑GAGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGa                                                                                       <  2:78479/233‑1 (MQ=255)

                                                                                                                                                         ttccc‑GGGTTTTAA‑AGAGGA‑AAAACCAAGTCC‑GGGCCAG‑GCAAAA‑CACACA‑CCAGAAAAAAAAAGAAGAAA‑A‑GCAGAAATCTTAAGTGAATCCGGACGCACCAT‑GCGGTTACGCCAATAACCACC‑AGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGa                                                                                       <  2:33074/236‑1 (MQ=255)

                                                                                                                                                         tgtCCGGGG‑TTAAA‑AGATGA‑AAAACAAAGTTC‑GGCCCAG‑GCAGAC‑ACTAAC‑CAAAAAAAAAAAAAAAGGAC‑A‑GCAGAAACGTAAAGAAAATCACGACCACAGAA‑GCGGTTACTGCAATACCCACA‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGa                                                                                       <  2:161259/239‑1 (MQ=255)

                                                                                                                                                          gtCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGGGTTGGCCGCGGATTCACTTAAGTTTTGTGGAGCAAAAAAAACGGCATTGAg                                                                                          >  2:46764/1‑234 (MQ=255)

                                                                                                                                                           tCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTc                                                                                    >  1:15277/1‑239 (MQ=255)

                                                                                                                                                             c‑GGT‑TTTAT‑AGAGG‑‑AAAACCTAATTT‑TGGCCCC‑GGACAACCCTACA‑CAAAAAAAAAAAAACGGGAC‑A‑CCAACACTTGTAATGAAATCACAACGCGCGAT‑GCGTTTACTGCAAAACCCCCA‑GGGGTCACCGCCGATGATGCAACCAGTCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGCCTCaa                                                                                  <  2:1068982/239‑1 (MQ=255)

                                                                                                                                                             c‑GGG‑TTTAA‑AGAAGA‑AACACCAAGTTC‑GGGACAG‑GCAAAC‑CCAACA‑CCAAAAAAAAAAAACAGAAC‑A‑GCACAAATTTAAAGTGAACCCCGACGCCCGAA‑GGGTTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCaa                                                                                  <  2:879854/239‑1 (MQ=255)

                                                                                                                                                              tcctggttttaaaaggaaaaaaCCATGTTC‑CGGCAAG‑GAAGAC‑ACAACC‑CAAGAAAAAAAAAACAGGAC‑G‑GCAGAACTCATAGGTAACTCCCAAAGCCAGAG‑GGGGTTACTGCAAAACCCCCC‑GAGACAACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGTAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACt                                                                                     <  2:1050596/221‑1 (MQ=255)

                                                                                                                                                                 g‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGtctgga                                                                                           >  1:928574/1‑222 (MQ=255)

                                                                                                                                                                  aaaaaagaagggT‑AAAACCAATTTC‑GGCCCAG‑GAAAAA‑CCTACC‑CCAGAAAAAAAAAACAGGAC‑A‑GCAGCAATCTAAAGTGAACCCCGACGACCGAA‑GCGGTTACTGAAATACCCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACa                                                                                <  1:300130/229‑1 (MQ=255)

                                                                                                                                                                             gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAACACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                                                                                          >  1:1077944/1‑218 (MQ=255)

                                                                                                                                                                             gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCCTTAAGTTTTGGGGAGCAACAAAGACGGAATTGAGGGACCCAAAAGGACCGGaaaa                                                                     >  2:671499/1‑238 (MQ=255)

                                                                                                                                                                             gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGAGGGAATTGAGGGAATCAAAAGAACCGGaaaa                                                                     >  2:806720/1‑238 (MQ=255)

                                                                                                                                                                             gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGGATTGAGGGACTCAACAGCACCGGaaaa                                                                     >  2:311430/1‑238 (MQ=255)

                                                                                                                                                                             gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTAACTTAAGTTTTGTGGAGCAACAAAGACGGAATTGAGTGACTCAAAAGAACCGGAAAt                                                                     >  1:201332/1‑239 (MQ=255)

                                                                                                                                                                             gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAACGGATTCACTTAAGTTTTGTGGACCAACAAAGACGGATTTTAGGGAACCAAAAGTACCGGaaaa                                                                     >  2:520615/1‑238 (MQ=255)

                                                                                                                                                                                        gTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCCTTAAGTTTTGTGGGGCAACAAAGACGGTATTGAGTGACTCAAAAGGACCGGAAAAAAcggcgggg                                                           >  2:74051/1‑237 (MQ=255)

                                                                                                                                                                                        gTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGGGCAACAAAGACGGAGTTGAGGGACTCAACAGTACCGGAAAAAAcggagggg                                                           >  2:116144/1‑237 (MQ=255)

                                                                                                                                                                                        gTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGGATTGAGTGACCCAACAGTACCGGAAATAAcggcgggg                                                           >  1:823695/1‑237 (MQ=255)

                                                                                                                                                                                        gTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAAACGGTATTGAGTGAACCAAAAGAACCGGgaaacggtccctta                                                           >  2:300130/1‑228 (MQ=255)

                                                                                                                                                                                                    agacaggaaccccccccAAAAAAAAAAAAAACGCC‑A‑GCACAAATCTAAAGGAAACCCCGACG‑CCGAAGGCGTTTAGGGCAAAACCCCCC‑GGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGc‑c                                                  <  2:571228/224‑1 (MQ=255)

                                                                                                                                                                                                             caaca‑cAAAAAAAAAAAAACAGGAC‑A‑GCAGCAATCTAAAGGGAACCCCGACGACCAAA‑GCGGTTACGGCAATACCCCCC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATaaa                                        <  1:311430/237‑1 (MQ=255)

                                                                                                                                                                                                             cTACA‑CCAAAAATAAAAACGAGGAC‑A‑GCAGAAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATaaa                                        <  1:358730/239‑1 (MQ=255)

                                                                                                                                                                                                             cTAAA‑CCAAAAAAAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGCCGACCGAG‑GGGGTTACTGCAATACCCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATaaa                                        <  1:654970/239‑1 (MQ=255)

                                                                                                                                                                                                              ccaccccAAAAAAAAAAAAC‑GGACAA‑GAAACAATTTTAAGTAAACCCCGCGCCCCGAA‑GCGGATACTCAAAAAACCCCC‑GGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATaaa                                        <  2:95857/234‑1 (MQ=255)

                                                                                                                                                                                                                ca‑cCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGAATTGGGTGACTCAACAGTACCGGAAATAAaggcggggacggggcaaaaaaaaaaaaa                                       >  2:710395/1‑216 (MQ=255)

                                                                                                                                                                                                                 a‑cCAAAAATAAAAAACAGGAC‑A‑GCAGAAATCTTAAGGAAATCCCGGCGACCGAA‑GCGTTTACTGAAATACCCACC‑GGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAaccac                                    <  1:1082042/239‑1 (MQ=255)

                                                                                                                                                                                                                 a‑cCAAAAATAAAAAACAGAAC‑A‑ACAGCAATCTTAGGTGAACCACGAGGACCGAA‑GCGGTTACTGAAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAaccac                                    <  1:763493/239‑1 (MQ=255)

                                                                                                                                                                                                                   ccAAAAAAAAAAAAAGGGGC‑A‑CCAAAATTCTTAAGAGAACCACGACGACCGAA‑GGGGTTACGGAAAAAACCCCC‑GGGGCCCCCGCCGATGATGCAACCAATCAGAGCGCTTGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGATATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAaccacc                                   <  2:84246/239‑1 (MQ=255)

                                                                                                                                                                                                                     aGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGCCTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCACaaaaaaaaaaaaacaa                                  >  2:64796/1‑233 (MQ=255)

                                                                                                                                                                                                                     aGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGGCGGAGC‑Caaaaaaaaaaaacggct                                 >  1:571228/1‑232 (MQ=255)

                                                                                                                                                                                                                     aGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGATTTGTGGAGCGACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGGGTCGGAGA‑CAAATAATAAaacaccca                                 >  1:527742/1‑237 (MQ=255)

                                                                                                                                                                                                                       aaaTAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                                                                                          >  1:634867/1‑181 (MQ=255)

                                                                                                                                                                                                                         aTAAAAAACAGGAA‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAACCACCTAATGt                             <  1:64796/239‑1 (MQ=255)

                                                                                                                                                                                                                          tAAAAAACGGAAC‑A‑GAAGAAATTTTAAGGGAACCCAGGCGACAGAA‑GGGGTTACGTAAATACCCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAACCACCTAATGTa                            <  2:511968/239‑1 (MQ=255)

                                                                                                                                                                                                                            caggtggttaaaagaaacagaTCTTAAGTAAATCCCGACGACCAAA‑GGGGTTCACGCAAAACCCCCG‑GAGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAACCACCTAATGTa                            <  2:851148/219‑1 (MQ=255)

                                                                                                                                                                                                                                               aaTCTTAGGGGAATCACGACGTCCGAA‑GGGTTAACTGCAATACCCACC‑GGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGa           <  1:119552/237‑1 (MQ=255)

                                                                                                                                                                                                                                                aTCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGGAGC‑CAAAACAAAAAACCCCTAATTTAGGGACTACAGGAATCGcaaa        >  1:711415/1‑239 (MQ=255)

                                                                                                                                                                                                                                                      aGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTGGTAGC‑CCAATAATAAACCACCTAATGTAACGACTAAAGTAAACGGAAAaatata  >  1:676215/1‑239 (MQ=255)

AAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATAT‑ACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACA‑CCAGAAATAAAAAACAGGAC‑A‑GCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGC‑CAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATA  >  NC_000913/4241757‑4242229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`