BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,285,483	A→G	12.7%	S630P (TCG→CCG)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,483	0	A	G	12.7%	336.5 / 7.9	147	S630P (TCG→CCG)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (69/54); new base G (4/18); total (75/72)
Fisher's exact test for biased strand distribution p-value = 1.09e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 148 total aligned reads displayed.

AATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGC  >  NC_000913/4285248‑4285718

ttctCCCGCTCCCTTATGGGGGAAGTTTACCTCCCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGt                                                                                                                                                                                                                                           <  2:532311/236‑1 (MQ=255)

aaTTCCCGGTCCTTGATGGGGGAAGGTTAACGGTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGt                                                                                                                                                                                                                                           <  2:228145/239‑1 (MQ=255)

      cGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGCCTACGCCAGGATCGGGAAAGGTCGAGGTATCGc                                                                                                                                                                                                                                      >  2:813338/1‑238 (MQ=255)

      cGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGcc                                                                                                                                                                                                                                     >  1:1044715/1‑239 (MQ=255)

                     gaAGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGGCGAGGTATCGCCCAGGTTGCTGG‑TAt                                                                                                                                                                                                                      >  1:214654/1‑238 (MQ=255)

                     gaAGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGGCGAGGGATGGCCCAGGTTGCTGG‑TAt                                                                                                                                                                                                                      >  1:1033230/1‑238 (MQ=255)

                     gaAGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAACCTTCGAGGAATCGCCCAGGTTGCTGG‑AAt                                                                                                                                                                                                                      >  1:665012/1‑238 (MQ=255)

                      aaGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCACCGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGCTGGAATCGCG‑ATAGCCTGCTTCTCTTCAACCAGCTTCTCGCCTTCGCCCGGATGGCCCACAGCCGAGGTATCCCCCCGGTTGCgggatac                                                                                                                                                                                                                      >  2:114920/1‑234 (MQ=37)

                       aGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGTTGGAATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACCCCCGGAGCGGCAAGCGTCGGGGTAACCCCCAGGTTGTGGG‑GATcg                                                                                                                                                                                                                    >  2:321554/1‑238 (MQ=38)

                         gTTACCGCTCGGTTACCCCTTGCCAAAGTGT‑GGCCGAAAAAGGCGTTTACCCCCCATCCGGCAATCAATTCCTGAGGCGACGCTGTCGCGTCTTATCGGGCCTACAAACCGTTCCCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATcgcc                                                                                                                                                                                                                  <  2:299361/238‑1 (MQ=255)

                           tAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGGGGTATCGCCCAGGTGGCTGG‑GTAcgcccg                                                                                                                                                                                                                >  1:804332/1‑238 (MQ=255)

                             aCGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAACGTCGAGGTATCGCCCAGGTTGCTGG‑TATcgccagcc                                                                                                                                                                                                              >  1:812790/1‑238 (MQ=255)

                                   gggTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGACCGGCAAGGGTCGGGGTATCGCCCAGGGTGCGGG‑TGTCCCCCGCCCCAAttt                                                                                                                                                                                                       >  2:840040/1‑239 (MQ=38)

                                    ggTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGAATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAAtttt                                                                                                                                                                                                      >  1:636659/1‑239 (MQ=255)

                                         cccTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑CTAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTCCGCCAGGATGGGCAAGCGTCGAGGGACTGCCCAGGGTGCTGG‑TATCCCCCCCCCCCAtttttccga                                                                                                                                                                                                 >  2:406414/1‑234 (MQ=38)

                                             tGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAAt                                                                                                                                                                                             >  1:603138/1‑239 (MQ=255)

                                              gCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCCGGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATa                                                                                                                                                                                            <  1:36539/239‑1 (MQ=255)

                                                     tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGATTACGCCAGGATCGGCAAGCGTCGAGGCATCGCCCCGGCTGTTGG‑TATCGCCCGCCGCACTTTTGCGCGGAATAcgccgc                                                                                                                                                                                      >  1:476373/1‑239 (MQ=38)

                                                     tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGAACGGCAAGCGTCGAGGGATCGCCCCGGTTGCTGG‑ATCCGCCCACGGCAATTTTGCGCCGaaaaaggggc                                                                                                                                                                                      >  2:392932/1‑231 (MQ=38)

                                                       tcgggcGGATAAGGCGTTTACGCCGCCTCCGGCAATCAATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCAt                                                                                                                                                                                    <  1:89826/234‑1 (MQ=255)

                                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATaa                                                                                                                                                                                  >  1:591517/1‑239 (MQ=255)

                                                                    gCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                                                                                       >  2:381426/1‑239 (MQ=255)

                                                                     gttttcccccccctCCGGCACACAATGCGTGACGGGACGCCGTCGGGTCTTAACAGGCCTACAAACCGTTACGGACTGGCATCGGGCAATTGTGGGTTACGATGGCTGCGCG‑ATGGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGc                                                                                                                                                                      <  2:598421/226‑1 (MQ=255)

                                                                     gttttccccccattCCGGCAACAAATGCCTGATGCGCCGCGGTCGCGTCTTATCAGGCCTAAAAACCGTTACCGACGCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                                                                                       <  2:908389/225‑1 (MQ=255)

                                                                      ttttcccccgcTTCCGGCAATCAATGCTGGAGGGGACGCTGGTGCGTCTTATCAGGCCTACAACCCTTTCCCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGc                                                                                                                                                                      <  2:68590/231‑1 (MQ=255)

                                                                      ggttgcgccgcATCCGTCAATCAATGACTGATGGGACGCTGTCGCGTTTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGGGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGc                                                                                                                                                                      <  1:406414/233‑1 (MQ=255)

                                                                      ctttagcccgcACCCGGCAATCAATGCCTGAGGCGGCGCTGTCGCGTCTTATCAGGCCTACAAGCCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑AGAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGc                                                                                                                                                                      <  2:659571/231‑1 (MQ=255)

                                                                        ttACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGcc                                                                                                                                                                                                                                     <  1:893004/174‑1 (MQ=255)

                                                                         tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGCGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTTCCGGacggggg                                                                                                                                                                  >  2:253049/1‑233 (MQ=255)

                                                                         tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGt                                                                                                                                                                  >  1:576041/1‑239 (MQ=255)

                                                                              cgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGGGCATAATTTTTCAGG‑GCGGGGTTTaag                                                                                                                                                            >  2:690929/1‑237 (MQ=255)

                                                                               gcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCATCTTCTCGACTACGCCCGGATCGGCAAGCGGCGAGGGATCGCCCAGGTTGCTGG‑CAACGCCCCCCGCCATTTTGCCACGAAAACGGGCCATCATTTTGACGGAGGGGGGTTTTgg                                                                                                                                                            >  2:573529/1‑239 (MQ=37)

                                                                                    cGGCAATCAATGCCTGATGCGACGCTGTCGCCTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCCCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAg                                                                                                                                                         <  1:690929/237‑1 (MQ=255)

                                                                                        aatcaatGCCTGATGCGCCGCTGTCGCGTCTTAGCAGGCCTACAAACCGTTCCCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                                                                                    <  1:65090/238‑1 (MQ=255)

                                                                                                    aTGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGCGGTATCGCCCAGGTTGCTGG‑TATCGCCCCCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGACAGGGTTTTAGGGAGGGGGTCGGTCCCGGgca                                                                                                                                        >  2:729485/1‑238 (MQ=255)

                                                                                                    aTGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑AATCGCCCCCCGCAATTTTGCCCAGAATACGGCGCATAATTTTGCCGGAGGGGGTTTTAGGCAGGGAGAGGGTCAAGGgcag                                                                                                                                       >  2:671250/1‑239 (MQ=255)

                                                                                                        gACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCTTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCGGAATACGGCGCATAATTTTGCCGGGCGGGGGTTTAGGcgagggtgcggccccgggagcacg                                                                                                                                    >  2:812366/1‑215 (MQ=255)

                                                                                                        gACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGGCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCCGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCCGTGCAGCACg                                                                                                                                   >  1:272864/1‑239 (MQ=255)

                                                                                                                 gcgTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCCTAATTTTGCCGGAGGGGGGTTTAGGCAGGGAAGCGGGCCAgggaggccagtctgggggc                                                                                                                          >  2:1012010/1‑221 (MQ=255)

                                                                                                                    tCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTCCAGGACGTCTGGCGGCGcc                                                                                                                       >  1:422659/1‑239 (MQ=255)

                                                                                                                         tCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGGCGGGCCAGGGAAGCACGTCTGGGGGCGcccgggg                                                                                                                  >  2:348441/1‑234 (MQ=255)

                                                                                                                                tACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGGGCGGGGTTTAGGCCGGGAGTCGGTCCAGTGCGGCCCGTCTGGCGGCGCCAGCGGGCCaa                                                                                                             >  1:443759/1‑237 (MQ=255)

                                                                                                                                  cAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATCATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGGGCAGCACGCCTGGCGTCGCCACCGGGCCaaac                                                                                                           >  2:328075/1‑235 (MQ=255)

                                                                                                                                  cAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGCTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAAtc                                                                                                           >  1:339229/1‑237 (MQ=255)

                                                                                                                                         ttACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATACTTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCCCGTCTGGCGGCGCCCGCGGGCCAATCTCTTTACGc                                                                                                  >  2:271269/1‑239 (MQ=255)

                                                                                                                                            ccgcccccctcccggaattggggggTTCCGTTGGCATCGCC‑ATAGCCTGCTTCTTCTCAAGCGGCTTTCCGACTACGCCAGGCTCGGCAAGCGCCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                                                                                               <  2:361531/217‑1 (MQ=255)

                                                                                                                                            ccGACCCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCCCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                                                                                               <  1:862978/239‑1 (MQ=255)

                                                                                                                                               aCTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTgcg                                                                                                                                                                                                   <  2:336956/136‑1 (MQ=255)

                                                                                                                                               aCTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTgcg                                                                                                                                                                                                   >  1:336956/1‑136 (MQ=255)

                                                                                                                                                    cATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGACCTGTGCAGCACGCCGGGGCCCGCCACGGGGCCCATCTCTTTCCCCCCCCaagatgg                                                                                       >  2:847870/1‑233 (MQ=255)

                                                                                                                                                      tCGGGCAAGTGGGGGTTACGATGGCATCGGG‑ATAGCCTTCTTCTCTTCAAGCTGCTTCTCGACTACGCCAGGAACGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                                                                     <  1:669128/239‑1 (MQ=255)

                                                                                                                                                      cggggCATTGGTGGGTTACGA‑GGCTTCCCGTATAGCCTGCTCTTCTTAAAGCCGCTTCTCGACTACGCCAGGAGCGGAAAGCGTCGGGGTACCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                                                                     <  2:591517/237‑1 (MQ=255)

                                                                                                                                                      ccGGGCTTTTGGGGGTTACGTTGGCAGCGCG‑ATCGGCTGCTTCTCGTGAAGACGCTTCTCGACGACGCCGGGATGGGAAAGCGTGGAGGTATCGCCCGGGTTGCTGG‑TACCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                                                                     <  2:881654/238‑1 (MQ=255)

                                                                                                                                                         ggCAATTGTGGTTTAGGGGGGCATCGCG‑ATGCCCGTCTTTTCTGCAAGCGCCTTCTCGCCTGCGCCAGTCTGGGCAAGGGACGGGGTATCCCCCCGGTTTCTGG‑TAGCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACtt                                                                                  <  2:443759/239‑1 (MQ=255)

                                                                                                                                                         ggCAATTGGGGGTTACGATGGCAACGCG‑ATAGCCTTCTTCTTTTCAAGCAGCTTCTCGACCACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACt                                                                                   <  1:729485/238‑1 (MQ=255)

                                                                                                                                                            tgttgtgttttgcgtgggCGTCGCG‑CTCGCCTGCTTCCCTTCAAGCCGCTTTTCGACCTCGCCAGGTCGGGCAAGGCTCGGGGTATCCCCCAGGTTCCGGG‑TTTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCt                                                                                <  2:129445/222‑1 (MQ=255)

                                                                                                                                                                   ggTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCAAGTTGCGGACTTCTCCGTACAg                                                                        >  1:966786/1‑239 (MQ=255)

                                                                                                                                                                    gTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                                                                                       >  1:37960/1‑143 (MQ=255)

                                                                                                                                                                        cGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCCGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACCCACCCAGTTGCGGGCTTCCGCGTACAGTTCTg                                                                   >  2:295169/1‑239 (MQ=255)

                                                                                                                                                                        cGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTATGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGCCTGGCGTCGCCACCGGGCCAATCCCTTTTCCCACCCACTTGCGGACTTCTGCGTCAAGTTCTg                                                                   >  2:89936/1‑239 (MQ=255)

                                                                                                                                                                            ggCATCGCG‑ATGGGCTGCTTCTCTTCAAGCAGTTTCCCGACTCCGCCAGGATCGGCAAGCGTCGAGGTATCGCCCGGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGa                                                               <  1:248827/239‑1 (MQ=255)

                                                                                                                                                                            ggCATCGCG‑ATATCCGGCTTCTTGTCAAGCAGCTTCGCGACTACGCGAGGAGCGGGAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGa                                                               <  1:392932/239‑1 (MQ=255)

                                                                                                                                                                             gCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGa                                                               <  1:326358/238‑1 (MQ=255)

                                                                                                                                                                             gCATCCGG‑AAAGCCGGCTTCCTTTCAATCACCTTTCGGACTACCCCGGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGa                                                               <  1:831818/238‑1 (MQ=255)

                                                                                                                                                                               aTCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACgg                                                            >  1:290512/1‑239 (MQ=255)

                                                                                                                                                                                     gttgcccTTCTTCCTTTCAAGCGGCTTCTGGACTCCGACAGGATCGGCAGGCGTGGGGGCATCGCCCAGGTTGCTGG‑TAGCGCCCGGCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtcct                                                       <  2:193223/234‑1 (MQ=255)

                                                                                                                                                                                       atgcctgttctcctTCAAGCAGCTTCTCGACTGTGCCAGGATCGGCAGTCGTCGAGGTATCGTCCAGGTTGCTGT‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtcctcc                                                     <  1:72483/227‑1 (MQ=255)

                                                                                                                                                                                         gcttgtttttcttctAGCAGCTTCTCGCCTCGGCCAGGACCGCCAAGCGGGGGGTTATCGCCCAGGTTGTTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATCCGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTcccc                                                   <  2:1044715/230‑1 (MQ=255)

                                                                                                                                                                                         gcttGCTTCTCTTAAAGCCGCTTCTCGCCTCCCCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TACCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTcccc                                                   <  1:433359/236‑1 (MQ=255)

                                                                                                                                                                                           tctgttCTCTCAAAGCCGTTTCTCGACTGCCCCAGGACGGGCAAGGGGCGAGGTTCCGCCAGGTTTGCGGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTcccc                                                   <  1:391605/233‑1 (MQ=255)

                                                                                                                                                                                              cTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTGTGCGTACAGTTCTGGTGACGGTTCCTcccccggat                                              >  1:13965/1‑234 (MQ=255)

                                                                                                                                                                                              cTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGAt                                              >  1:231454/1‑239 (MQ=255)

                                                                                                                                                                                               ttCTCTTCAAGCAGCTTGTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCTGGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGAtt                                             <  1:541486/239‑1 (MQ=255)

                                                                                                                                                                                               ttCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGAtt                                             <  1:804955/239‑1 (MQ=255)

                                                                                                                                                                                                  cctTCAAGCAGCTTCTCGACTCCGCCAGGATCGGAAAGCGTCGAGGTTTCGCCAAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAg                                          <  1:253049/238‑1 (MQ=255)

                                                                                                                                                                                                    tgcaagcgtgttCGCGACTGCGCCAGGATCGGCAAGGGTCGGGGTAGCGCCCGGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCg                                        <  1:169663/229‑1 (MQ=255)

                                                                                                                                                                                                           agcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACg                                   <  1:1037297/237‑1 (MQ=255)

                                                                                                                                                                                                                ctcGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCg                                                                             >  2:1049194/1‑190 (MQ=255)

                                                                                                                                                                                                                  cGACTACGCCGGGATCGGCAAGCGTCGAGGTACCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAg                          <  1:840040/239‑1 (MQ=255)

                                                                                                                                                                                                                       aCGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAAtc                                                                                                           >  2:887534/1‑153 (MQ=255)

                                                                                                                                                                                                                        cGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTCCAGTTCTGGGGCCGGTTCCTCCCCGTGCTTAAGCGGGCCGAGGCCGTAGATccc                     >  2:372425/1‑236 (MQ=255)

                                                                                                                                                                                                                        cGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCg                      >  1:234617/1‑237 (MQ=255)

                                                                                                                                                                                                                        cGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGGGACGGTTCCTCCCCGCGATTAAGCGGGACGTAGGCGTAGATCg                      >  1:82745/1‑237 (MQ=255)

                                                                                                                                                                                                                          gcaggtcgggCAAGCGGTGGGGTACCGCCCGGGTGGGGGG‑TAGAGCCGTCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  2:757016/231‑1 (MQ=255)

                                                                                                                                                                                                                          cccggttgggCATGCGTTGGGGTATCGCCCGGGTT‑CTGGTTCCCGCCCGCCGCAATTGTGCGCAGAATACGGCGCATAATTTGGCCGGCGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  2:221573/231‑1 (MQ=255)

                                                                                                                                                                                                                          cccggACCGGCAAGGTGCGAGTTTTCGCCCGGGTTGTTGG‑TCTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGTGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  2:422659/236‑1 (MQ=255)

                                                                                                                                                                                                                          ccAGGGTCGGCCAGCGTCGGGGTATCGCCCAGGTTGCTGG‑GTCCGCCCGCCGCAATTTTGCGCAGAATGCGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  1:955000/239‑1 (MQ=255)

                                                                                                                                                                                                                          ccAGGATGGGTAAGCGGCGGGGTCTGGCCCAGGTTGCTGG‑TATCCCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  1:107470/239‑1 (MQ=255)

                                                                                                                                                                                                                          ccAGGATGGGCAAGGGGCGGGGTATCGCCCGGGTGTCTGG‑TATCGCCCGGCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  1:582006/239‑1 (MQ=255)

                                                                                                                                                                                                                          ccAGGATCGGCAAGCGTGGAGGTACCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  1:321554/239‑1 (MQ=255)

                                                                                                                                                                                                                           ccggATGGGCAAGTGTCGGGGTATCCGCCGGGTTGGTGT‑TGCGGCCCGCCGCAATTTTGCGCAGAATACGGCGGATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  2:476373/236‑1 (MQ=255)

                                                                                                                                                                                                                           cAGGTTCGGCAAGCGTCGGGGTTACGGCCAGGTTGCTTG‑TATCGCCCGTCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  1:114920/238‑1 (MQ=255)

                                                                                                                                                                                                                           cAGGATCGGCAAGCGTCGAGGTCTCCCCCGGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTg                  <  1:813338/238‑1 (MQ=255)

                                                                                                                                                                                                                               atgggCAAGGGTCGGGGTATCGCCCGGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTATGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGAc                <  1:89936/233‑1 (MQ=255)

                                                                                                                                                                                                                                ctgggaggCGTGGGGGTATCGCCCGGTTTCGTGG‑TGTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGGGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCtt             <  2:315352/231‑1 (MQ=255)

                                                                                                                                                                                                                                   gCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCACCGGGCCAATCTTTTTACGCACCCAACTGCGGACTTCTCGGTCCAGTTCTGGTGACCGTTCCCCCCCGCAGTTAAGCCTGACGTAGCGGCATCTCGCCTGCCCTTTAat         >  2:1061897/1‑239 (MQ=255)

                                                                                                                                                                                                                                      aGCGTCGAGTTAGCCCCCAGGTTGCGGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATAtt      <  1:694455/239‑1 (MQ=255)

                                                                                                                                                                                                                                        cGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATAtttt    >  1:19104/1‑237 (MQ=255)

                                                                                                                                                                                                                                          tgtaGGTATCGCCGGGGTGGCTGG‑TTTGCCCCGGCGCAATTGTGCGCAGAATACGGCGCATAATTGTGCCGGAGCGGGTTTTAGGGAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGc  <  2:1010496/236‑1 (MQ=255)

                                                                                                                                                                                                                                          tCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGGGACGGTTCTTCCCCGGGATTAAGCGTGCCGAAGGCGTAGATCGCCTCACCTTTACTATTGTGc  >  1:561469/1‑239 (MQ=255)

AATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAA‑TGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCG‑ATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGG‑TATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGC  >  NC_000913/4285248‑4285718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`