BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,467,784	G→T	12.6%	L53F (TTG→TTT)	mgtA →	magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,467,784	0	G	T	12.6%	328.5 / 2.4	117	L53F (TTG→TTT)	mgtA	magnesium transporter
Reads supporting (aligned to +/- strand): ref base G (35/64); new base T (16/2); total (51/66)
Fisher's exact test for biased strand distribution p-value = 3.50e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 117 total aligned reads displayed.

TATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACGGAAGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTA  >  NC_000913/4467551‑4468013

 ttttccTTCAGGTAAGGTTTCCCCCCGCTTTAAGACATTTCTGTACTGTTTCAGACGGTGGGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCCTTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGaaa                                                                                                                                                                                                                                      <  2:551911/235‑1 (MQ=255)

  ttaCCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGGGAATACGGGGGGCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGGGAGGACCCAAGAAAAA‑ATTG‑GGaaaa                                                                                                                                                                                                                                     >  2:300355/1‑239 (MQ=255)

       ttCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGACAATGGCGGGGAGGCCCGACGCAGAA‑TTTT‑GGAAAACG‑TTc                                                                                                                                                                                                                               >  1:1001066/1‑239 (MQ=255)

             tAAGGCTTCGCCAGGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGacac                                                                                                                                                                                                                          <  1:456747/238‑1 (MQ=255)

             gtaGGCTTCGCCACGCCTCAAGCCTTTTCTGTACTTGTTCCGACTGTGCGGAGCGACTCCTTAGTGTTAAAGAAATTTTTACCCGGCTCATGCGCCATTTACCGTCCCGTCTGGTTCATCGTGATCCAATGCCTGGCGCGCAGCAGACAGTGAATACGGTTGTCCCGCCGTCCTTAATTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGacac                                                                                                                                                                                                                          <  2:613559/236‑1 (MQ=255)

                  cTTCGCCACGCCTGAAGACATTTCTGGACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCAt                                                                                                                                                                                                                      <  1:247515/237‑1 (MQ=255)

                    tCGCCACTCCTGAAGAAATTTTTGTACTGTTTCAGACAGTGGGGAGGGACTCCTTAGGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACACATCCgg                                                                                                                                                                                                                  <  2:279653/239‑1 (MQ=255)

                    tCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGACGGGGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCgg                                                                                                                                                                                                                  >  1:289906/1‑239 (MQ=255)

                     cGCCCCGCCTTAAGACTTTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGGCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGa                                                                                                                                                                                                                 <  2:188401/239‑1 (MQ=255)

                          cGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGCCAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGGTAGGCCCCGCGAAGAA‑TTGT‑GGAAAACGCTTGAACCCCCACCCGGAgggg                                                                                                                                                                                                             >  2:1065574/1‑239 (MQ=255)

                           gCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTCCCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGaca                                                                                                                                                                                                                           <  1:978550/223‑1 (MQ=255)

                                   tctttttggttcttttgccgACAGGTCGGAGGGCCTCCTTTATTTTAAAGAAATTTTTACCCGGACCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAAGACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACCCCCATCCGGAAGGGTTAAATCAgg                                                                                                                                                                                                   <  2:541083/220‑1 (MQ=255)

                                   ccATTTCTGGTGTTTTTCACACCGTGGGGAGGGCCTCCTTATGTTTAAAAAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTTTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAgg                                                                                                                                                                                                   <  2:118505/238‑1 (MQ=255)

                                     aTTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGGTTCCCCACGAAGAA‑ATGT‑TGAAAAAC‑ATGGAAACACACCCGGAAgggggaaaaaagggg                                                                                                                                                                                                 >  2:309442/1‑227 (MQ=255)

                                                 tttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGCAGAAGAA‑TTGT‑GGGAAAAG‑ATTGACCACCATCCGGCAGGGTTAAATCAGGCGGaaagggatgc                                                                                                                                                                                      >  1:412226/1‑230 (MQ=255)

                                                 tttCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAgg                                                                                                                                                                                                   <  1:721991/225‑1 (MQ=255)

                                                      gACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑ATTT‑GGAAAACG‑TTCGACAACAACCCGGAAGGGTTGAAACAGGGGGagagggaaaacacccg                                                                                                                                                                                >  1:605728/1‑224 (MQ=255)

                                                         aGTGCGGGGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCcgcg                                                                                                                                                                              <  1:284814/238‑1 (MQ=255)

                                                         aGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGAGGCCCGAAGACAAA‑TTGT‑GGAAAACC‑TTTGACACCCATCCGGAAGGGGTTAATCAGGCGGAAGTGGGAACTGCCCGCGa                                                                                                                                                                             >  1:328508/1‑239 (MQ=255)

                                                           tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGTGGCCCCAAGAAGAATTTGG‑GGAAAACG‑‑TCGCACCCCACCCGGCCGGGGGAAAACAGGGGGGAGGGGAAACTGccccgaaaa                                                                                                                                                                           >  2:202584/1‑233 (MQ=255)

                                                           tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGGTGCCCGGAGAAGAA‑TTTT‑GGAAAAAG‑TTCGAACCCCCTCCGGGAGGGGTAAATCAGGGCGAAGGGGaaatggcccttatac                                                                                                                                                                           >  2:721991/1‑226 (MQ=255)

                                                           tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCCGAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCCGGAGGGTTAAATACGGGGGGAGTGGGATCTTGACGCGaaa                                                                                                                                                                           >  1:224780/1‑238 (MQ=255)

                                                           tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGGGATGCCCGCGAAAGAA‑ATTT‑GGGAAAAG‑TTTGGCACCCCTCCGGCAGGGGTTAAACAGCCGGGAGGGGaacagcaccccgaca                                                                                                                                                                           >  2:284814/1‑226 (MQ=255)

                                                           tGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGGGATGCCCGAGGAAGAA‑TTTT‑GGAAAACG‑TTCGACACCCACCCGGAAGGGTTAAAACAGGGGGAAGGGGAAAACGCCCCCGaaa                                                                                                                                                                           >  2:963695/1‑238 (MQ=255)

                                                            gggggggggccctttttGTTTAAAGAAATTTTTCCCCGGCCCATTCCCCTTTTACCTTCCCGTTGGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGaa                                                                                                                                                                            <  2:755959/221‑1 (MQ=255)

                                                            gCGGAGGGACTCCTTATGTTTAAAGAAATTTTTGCCCGGCTCATTCGCCATTTTCCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCcgcg                                                                                                                                                                              <  1:202584/235‑1 (MQ=255)

                                                             cGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGGGGTGCCCGCAGAAGAA‑ATTT‑GGGAAAAG‑TTCGACACCCACCCGCGACGGTTAAATAAAGCGGAAGGGGAAtaagtccccgcacaa                                                                                                                                                                         >  2:84421/1‑225 (MQ=255)

                                                                    aCTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGa                                                                                                                                                                                                                             <  1:624221/180‑1 (MQ=255)

                                                                    aCTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGt                                                                                                                                                                  <  1:951107/238‑1 (MQ=255)

                                                                     cTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGCAGAAAAA‑TTGT‑GGAAAACC‑TTCGCCACCCCCCCCGAAGGGTTAAATAAGGCGGAGGTGGAAATTTCCcgccgacacaccggggg                                                                                                                                                                 >  1:117846/1‑225 (MQ=255)

                                                                     cTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGaaa                                                                                                                                                                                                                                      <  2:322273/171‑1 (MQ=255)

                                                                     cTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGaaa                                                                                                                                                                                                                                      >  1:322273/1‑171 (MQ=255)

                                                                      ctcTTATTGTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGaa                                                                                                                                                               <  1:17810/237‑1 (MQ=255)

                                                                          tATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGAGGGCCGACGAAGAA‑TTTT‑GGGAAACG‑TTCGGCACCCCACCGGAAGGGGTCAAAAAGGCGGGGGGGGGAACTGCCCCCGca‑caaaaagggggaaaaa                                                                                                                                                           >  2:951107/1‑226 (MQ=255)

                                                                                tAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTTT‑GGAAAACG‑TTCGaca                                                                                                                                                                                                                           >  2:734943/1‑170 (MQ=255)

                                                                                tAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGaca                                                                                                                                                                                                                           <  1:734943/170‑1 (MQ=255)

                                                                                tAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCACAGAAGAA‑CTTT‑TGAAAACA‑GTCGCCACCCCTCCCGGAGGGGTAAATAAGGGGGCAAGGGAAACTGCCCCCCAA‑CAAAACAGGGaaaaaaaaaaa                                                                                                                                                      >  2:367698/1‑235 (MQ=255)

                                                                                tAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGGGGTGCCCGCAGAAGAA‑TTGT‑GGGAAAAG‑TTTGAAACCCCTCCGCGAGGGGTAAATCAGGCGGGGGGGGGAACTGCCCGCGAA‑CAAAAAGGGGaaaaaaaaaaa                                                                                                                                                      >  2:963321/1‑235 (MQ=255)

                                                                                tAAAGAAATTTTTACCCGGCTCATTCACCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGCAGAAGAA‑TTGT‑GGAAAACG‑TTCGAAACCCCTCCGGAAGGGGTAAAACAGGCGGGAGGGGAACTTTCCCGCGAA‑CAACATGGGGGAAATAAATTa                                                                                                                                                      >  1:509646/1‑238 (MQ=255)

                                                                                 aaaGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTAcc                                                                                                                                                    <  1:397279/239‑1 (MQ=255)

                                                                                  aaGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGGGGAATCTGCCCGCGAA‑CAACATGGTGGAAATAAATTAcc                                                                                                                                                    >  1:18849/1‑238 (MQ=255)

                                                                                            cccccGGCTCTTTC‑CACTTTTCCTTCCGGTTTGGTTTTTTGTGATCCATTTCCGGGCGGCAAGGAGTAAGTGAATACGGTGGTCCCGCCGTGCTTAAGTGCGAATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAAcc                                                                                                                                        <  2:428784/237‑1 (MQ=255)

                                                                                                   cTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGGTGGCCGCAGAAGGA‑TTGG‑GGAAAACG‑TTCGCAACACATCCGGAAGGGGTAAATAAGGGGGGCATGGGATATGCCCGCGCA‑AAAACTGGGGGAAATAAAATAACCCAACaaaaacaccaccc                                                                                                                                  >  2:160706/1‑232 (MQ=255)

                                                                                                    tcctcccccATTTACCGTCCCTGCTGGTTCATCGTGATCCATTGGCTGTGGGGCAGCAGAAAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGcc                                                                                                                                  <  2:608917/231‑1 (MQ=255)

                                                                                                    cacttCGCCCTTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGcc                                                                                                                                  <  1:767694/235‑1 (MQ=255)

                                                                                                        tcgcgttttccctccccgttgttttCATCGTGATCACTTGCCGGGCCGGCAGCAGATAGTGAATACGGTTGTCCCGCCGGCCTGAAGTTCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGTGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCgtggt                                                                                                                             <  2:143096/216‑1 (MQ=255)

                                                                                                            tttttCCCTTCCCGTCTGGTTCATCGGGATCCCTTGCCTGGCGCGCAGCAGGCAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  2:122931/237‑1 (MQ=255)

                                                                                                            taagggtccgcccttgtGGTTCTTGGTGATCCATTGCCTGGGGGGCAGCAGACAGGGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  2:1040505/223‑1 (MQ=255)

                                                                                                            cttttACCTTCCCTTTTGGTTCATCGTTATCCATTGCCTGGCGCGCAGGAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTTATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  1:422074/237‑1 (MQ=255)

                                                                                                            ccttttCCTTCCCGTTTGGTTCATAGGGATCCATTGCCTGGCGGGCAGCAGGCAGTGAATACGGTTGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  2:905872/237‑1 (MQ=255)

                                                                                                            cctttCCCTTCCCGGCTGGTTCTGCTTGATCCATTGCCTGGGGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  2:808274/237‑1 (MQ=255)

                                                                                                            cATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  1:963321/239‑1 (MQ=255)

                                                                                                            cATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  1:978825/239‑1 (MQ=255)

                                                                                                            cATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  1:367698/239‑1 (MQ=255)

                                                                                                             aTTTCCCGTCCAGTCTGGTTCATCGTGAACCATTGCCTGGTGCGCAGCAGACAGTGAATGCGGTGGTCCCGCCGTCCGTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  1:519537/238‑1 (MQ=255)

                                                                                                             aTTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACC‑TTCGAACCCCATCCGGGAGGGGTTAATCAGGCGGAAGTGGAATCGGCCCCCGAA‑CAACAAGGGGGAAAAAAATTACCCGCACAACAACCCTCGcccgggggggga                                                                                                                        >  1:451209/1‑229 (MQ=255)

                                                                                                                    ttCCCGTCTTGTCCAGCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTAt                                                                                                                <  1:102785/239‑1 (MQ=255)

                                                                                                                      cccGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCCTCCGGAAGGGGTAAATACGGCGGAAGGGGAAACTGCCCGCGAACCAACTTGG‑GAAAATAAAATAACCCCCAAACAACACGCGCCGCGGGGGGGAC‑ATTTATgg                                                                                                              >  2:934632/1‑239 (MQ=255)

                                                                                                                       ccGTCTGGGTCTTCGGGATCCATTGCCTGGCGCGCAGCAGAAAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATggg                                                                                                             <  2:18849/239‑1 (MQ=255)

                                                                                                                              gTTCATCGTGATCCATTGCCTGGCGCGCAGCAGCCAGTGACTACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGCAAATGGCGGTGTTGCCCGCCAGCGAC‑ATGTGGGAAAGCG‑TTC‑CCCCCCCTCCCGTCGGGTTGAATCTATCCGGAGTGGAATCTTCCCCCCGC‑CCACATCATGGACATAAATTACCCCCCCCACAACCCTCGCCCGCGCGGGTGC‑TTTTAAGGGttctctt                                                                                                      >  2:1001411/1‑233 (MQ=255)

                                                                                                                                 cATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGGGAAAATAAATTACCCGCACAACAACCGCCCCCGTGGTGGGGAA‑AATTAttg                                                                                                              >  1:1040505/1‑226 (MQ=255)

                                                                                                                                   tCGTGCTCCATTGCCTGGCGCGCAGCAGAAAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCa                                                                                                <  1:475972/239‑1 (MQ=255)

                                                                                                                                   tCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGGTAAATCAGGCGGAAGTGGAGTCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGGGGGGAC‑ATTTATGGGTTTGC‑TATCGCa                                                                                                >  1:940096/1‑239 (MQ=255)

                                                                                                                                     tggATCCATTGCCGGGGGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGCGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAAc                                                                                              <  2:328508/237‑1 (MQ=255)

                                                                                                                                     gttaTCCATTGCCTGGCGCGCAGCAGACAGGGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAAc                                                                                              <  1:84421/236‑1 (MQ=255)

                                                                                                                                     gTGATCCATTGCCGGGCGCGCAGCAGACATTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAAc                                                                                              <  1:759093/239‑1 (MQ=255)

                                                                                                                                          ccATTTCCTGGGCCGCAGCAGAGAGGGAATGCGGGGGCCCCGCCGTCCTTAAGTGCGGATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCtt                                                                                         <  2:1001066/239‑1 (MQ=255)

                                                                                                                                          ccATTGCCTGGCGCGCAGTAGACAGGAAATACGGTTGTCCCGCCGGCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCtt                                                                                         <  1:488948/239‑1 (MQ=255)

                                                                                                                                            aTTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTa                                                                                       <  1:1065574/239‑1 (MQ=255)

                                                                                                                                               gCCTGGCGCGCAGCAGACAGGGAATACGGTGGTCCCGCCTTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAata                                                                                    <  1:233441/239‑1 (MQ=255)

                                                                                                                                                ccTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCg                                                                                                                                                                                                 <  1:753899/132‑1 (MQ=255)

                                                                                                                                                     cgcgcAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCCCAACAACCGTCGCCCCGGGGGGTGC‑ATTTCTGGGTCTTC‑TATCGCCACCCCTTTAATAtttt                                                                                >  1:597404/1‑237 (MQ=255)

                                                                                                                                                     cgcgcAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGAGGTGGCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGAAACCCATCCGGAAGGGGTAAATACAGCGGAAGGGGCAACTCCCCGCCAA‑CACCATGGGGGAAATAAATTACCCGCCACACAACCCTCCCCGTGGGGGGGAC‑AATTTTGGGGCGGC‑CATCTCAACCCCCTTCATAtttttc                                                                              >  2:829179/1‑237 (MQ=255)

                                                                                                                                                     cgcccAGCAGACAGGAAATACGGGGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGG‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTa                                                                               <  2:117846/238‑1 (MQ=255)

                                                                                                                                                                cggttaaTTCGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTTAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATtct                                                                    <  2:451209/233‑1 (MQ=255)

                                                                                                                                                                    gagtgtgtggttCCCGCCTGCCTTAAGTGCGCATTGCCTTAAAAGGGCGGTGATGCCCGAAAAAGAA‑TGTT‑GGAAAACT‑TGCAACCCCCATACGGAAGGGTGAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTCCCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATGTTACTCCCCATTCTCgg                                                                 <  2:536834/226‑1 (MQ=255)

                                                                                                                                                                    gAATACGGTGGTCCCGCCGTCCTTAAGTGCGGATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATtct                                                                    <  1:58902/234‑1 (MQ=255)

                                                                                                                                                                    gAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCCAAGAAGAA‑TTTT‑GGGAAACG‑TTCGACACCCATCCGGAAGGGGTAAATCAGGCGGAAATGGAATCTGCCCGCGAA‑CAAACAGGTGAAAATAAAATAACCGCCCAACAACCCGCGCCCTGGAGGGGCC‑ACTTTTGGGGATTA‑CATTCCAACCCCCTTAAAATTTTCATCACCATtctccgg                                                                >  2:82251/1‑235 (MQ=255)

                                                                                                                                                                     aaTACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGt                                                                                                                         <  1:642377/182‑1 (MQ=255)

                                                                                                                                                                     aaTACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTc                                                                            <  1:130302/225‑1 (MQ=255)

                                                                                                                                                                     aaTACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGgcg                                                               <  1:165390/238‑1 (MQ=255)

                                                                                                                                                                      aTACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGGGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCCCAAAAACCGTCGCCGTGGGGGGGAC‑ATTTATGGGTCTGC‑TATCGCAACCCCCTTAATATTTTACTCACCATTCTCGGCGCt                                                             >  1:181252/1‑239 (MQ=255)

                                                                                                                                                                      aTACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGGTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGGGAAAATAAATTAACCGCCAAACAACCGTCGCCGTGGTGGGTAA‑ATTTATGGGGCTGC‑TATCGCAACCCCCTTAATATTTTACTCACCCtttctgggcgt                                                             >  1:930452/1‑230 (MQ=255)

                                                                                                                                                                           gtggtCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGGTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCCCCGGGGGGGGTAC‑ATTTATGGGTCTGC‑TATTGGCACCCCCTTAAAATTTTACTCACCATTTTCgggcccatttt                                                        >  1:830240/1‑230 (MQ=255)

                                                                                                                                                                             ggtCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGCGAAGCCCGAGAAAGAA‑TTGG‑GGAAAACG‑TTAGACACCCCTCCGGAAGGGGTAAATAAGGCGGCAAGGGAATCCGCCCGCGGA‑AAACATTGTGGAAAAAAATTACCCCCACAACAACCGCCCCCCGGGGGGGGACGACTTATGGGT‑TGCTTTTCGCACCCCCTTTAATTTTTTTCacacacctcccgccgattttttt                                                       >  2:799834/1‑220 (MQ=255)

                                                                                                                                                                               tCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGGTGCCCGAAGAAGAA‑TTTT‑GGAAAAAG‑TTCGACACCCCTCCGGAAGGGGTAAAACAGGCGGAAGTGGAAACTGCCCCCGAA‑CAACATGGGGAAAATAAAATAACCGCACAACAACCCGCGCCCTGGGGGGGAC‑AATTATTGGGTTCC‑TTTCCCCACCCCTTTTATATTTTATTCCCCATTCTCTgctgttccttttaa                                                    >  2:130302/1‑227 (MQ=255)

                                                                                                                                                                               tCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTTT‑GGAAAACG‑TTCGAAACCCATCCGGAAGGGGTAAATCAGGCGGAAGTGGAATCTGCCCCCGAA‑CCACATGGGGAAAATAAATTACCCGCCCAAAAACCCTCGCCGGGGGGGGGGC‑ATTTTTGGTGCTCC‑TATCGCAAACCCTTTAATATTTTACTCCCCATTTTCGGCGCTATTTTTT‑Ac                                                   >  2:253214/1‑239 (MQ=255)

                                                                                                                                                                               tCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTTG‑GGAAAACG‑TTCGACAACCAACCGGAAGGGTTAAATAAAGCGGAAGTGGAATCTGCCCGCCAA‑AAACATGGTGGAAAAAAATTTACCGCACAACAACCCTCCACGTGGGGGGGAC‑AATTTTTGGTCTGC‑TATCCCCACCCCTTTCATATTTTTTTCACCATTTTCGGCGCCAttttttt                                                     >  2:179352/1‑237 (MQ=255)

                                                                                                                                                                                     cctCCTTTAGTGGGCATGTCCTGAAAATGGGGGGGATGCCCGAAGAAAAA‑TTGG‑GGAAACCG‑TTCGACACCCAACCGGAAGGGTTAAATCAGGCGGAAGTGGAATCGGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACg                                             <  2:553526/237‑1 (MQ=255)

                                                                                                                                                                                      gtcattaaggtggcATTGGCTGAAAATGGCGGGGAGGCCCGAAGAAGAA‑TTGG‑GGAAAACG‑TTCTACACCCATCCGGAAGGGTTAAATCAGGCGGAAGGGGAATCTGCACGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACgg                                            <  1:769882/227‑1 (MQ=255)

                                                                                                                                                                                         cTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTa                                                                                                                 <  1:641971/169‑1 (MQ=255)

                                                                                                                                                                                                 cgcATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGTAAACG‑TTCGACACCCATCCGGAAGGGGTAAATCAGGCGGAAGTGGAAACTGCCCGCGCA‑AAACATGGTGAAAATAAATTAACCGCACAAAAACCGTCCCCCCGGTGGGGGC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCCTACTCGGCGCGATTTCTT‑ACACCACGGAAAattttttt                                 >  2:759900/1‑239 (MQ=255)

                                                                                                                                                                                                   cATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAAAAACCCTCGCCGGGGGGGGGAC‑ATTTATGGGGATGC‑t                                                                                                      >  2:641971/1‑169 (MQ=255)

                                                                                                                                                                                                      tGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACGGAAGATTTATTtgctgc                            <  1:981359/239‑1 (MQ=255)

                                                                                                                                                                                                               aTGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACGGAAGATTTATTTGCTGCGGGCGTTAt                   <  1:300355/239‑1 (MQ=255)

                                                                                                                                                                                                                  gCGGGGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAAGGGTAAATCAAGCGGAAGTGGAAACTGCCCGCGGA‑CCACATGGGGAAAATAAATTACCCGCACAACAAACGTCGCCGCGGGGGGGAC‑ATTTATGGGGTTGC‑TTTCGGAACCCCCTTATATTTTTACTGCCCATTCTCAGCGCCTTTTCTTCACCCCAGGCAAGattattttttcggggcggtatgtg                 >  2:682850/1‑218 (MQ=255)

                                                                                                                                                                                                                            ccGAAGAAAGA‑TTGT‑GGAAAACT‑GTCGACACCCATCCGGAAAGGGTAAATCAGGGGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACGGAAGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTc      <  2:289906/239‑1 (MQ=255)

                                                                                                                                                                                                                            ccGAAAAAGAA‑TTGG‑GGAAAACG‑TTCGACACCCAACCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACGGAAGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTc      <  2:449475/239‑1 (MQ=255)

                                                                                                                                                                                                                             cGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACGGAAGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCg     <  1:138895/239‑1 (MQ=255)

                                                                                                                                                                                                                                cgaagaa‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCCCGGAAGATTTATTTGCTGCGGGGGTTATCCCGCTaacgtgcgact  >  2:690618/2‑230 (MQ=255)

TATTACCTTCAGGTAAGGCTTCGCCACGCCTGAAGACATTTCTGTACTGTTTCAGACAGTGCGGAGGGACTCCTTATGTTTAAAGAAATTTTTACCCGGCTCATTCGCCATTTACCTTCCCGTCTGGTTCATCGTGATCCATTGCCTGGCGCGCAGCAGACAGTGAATACGGTGGTCCCGCCGTCCTTAAGTGCGCATTGCCTGAAAATGGCGGTGATGCCCGAAGAAGAA‑TTGT‑GGAAAACG‑TTCGACACCCATCCGGAAGGGTTAAATCAGGCGGAAGTGGAATCTGCCCGCGAA‑CAACATGGTGAAAATAAATTACCCGCACAACAACCGTCGCCGTGGTGGGTAC‑ATTTATGGGTCTGC‑TATCGCAACCCCTTTAATATTTTACTCACCATTCTCGGCGCTATTTCTT‑ACGCCACGGAAGATTTATTTGCTGCGGGCGTTATCGCGCTAATGGTCGCTA  >  NC_000913/4467551‑4468013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`