Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	490,367	A→C	14.0%	V149G (GTG→GGG)	priC ←	primosomal replication protein N''

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	490,367	0	A	C	14.0%	192.3 / 2.4	76	V149G (GTG→GGG)	priC	primosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base A (43/18);  new base C (8/5);  total (51/25)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTTCATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGAGGCTTCACGCGCGATGGCTTCCAGTTGTGCCGCCAGATGTTCCGCCAGCCAGGCCACTTG  >  NC_000913/490143‑490602                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             cTTTTCCTATTCCCGTTTTACAATTCACAGGCCGGGCAGCCCCGTTCTGGCCGGGTTCCGATTTTCCTCCCGCAGCCACATCAAATCCACACGCAGTTTGACATCGTCAGGGGCATTTTCCAGTGTAATATTCTCTTCATTGCTAGCGGGTT‑AAGCGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATATGCTTCCACTTCACGATGCAGc                                                                                                                                                                                                                                <  2:324772/239‑1 (MQ=255)   tttCATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGTCAGCGTGCCAGGCGCGCTTCATAGGCTTCCCCTTCACCATGCAGCGt                                                                                                                                                                                                                              >  1:769028/1‑239 (MQ=255)         tattccccTTTAAAATTCCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCCGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTg                                                                                                                                                                                                                        <  2:251198/232‑1 (MQ=255)            cACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCCCAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACCCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCACCGTGCCAGGCGCGCTTCATTGCGTTTCCCTTCCCGAGGACGCGttttccttt                                                                                                                                                                                                                      >  2:569123/1‑232 (MQ=255)                 tttACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCCTAGGCTTCCCCTCCCCCATGCCGCCTTTCCTTTTCCCCgg                                                                                                                                                                                                                >  2:924877/1‑238 (MQ=255)                   tACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATCGGCTTCCACTTCACGCTGCAGCGTTTGCTGTTcccccag                                                                                                                                                                                                               >  1:91723/1‑233 (MQ=255)                       ttttCCGGCGCGCGCAGAACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCgg                                                                                                                                                                                                          <  2:840203/236‑1 (MQ=255)                             aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGCCAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGCTTTTTT‑CCAGCGC‑ATGGCGGCAGCGGTCCAGGCGCGCTTCATCTGCTTCCACTTCCCGCTTCAGCGCTTGCTGTTCCACGAGATCGGTCAccc                                                                                                                                                                                                    >  2:632724/1‑239 (MQ=255)                             aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGGTTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCCAGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCCGTTCCACGGGATCgggcccc                                                                                                                                                                                                     >  1:846609/1‑233 (MQ=255)                             aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCTAATGCGTCCAATTCACGATGCAACGTTTGTGGTTcccccatgtctgttcac                                                                                                                                                                                                     >  1:15858/1‑223 (MQ=255)                             aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAcc                                                                                                                                                                                                     >  1:137619/1‑238 (MQ=255)                             aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAcc                                                                                                                                                                                                     >  1:61584/1‑238 (MQ=255)                             aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGGGCCAGGCGCGCTTCCTAGTCTTCCCCTTCACGATGCCGCGTTTGCTGTTCCCCGAGATCGGTcccc                                                                                                                                                                                                     >  2:275024/1‑238 (MQ=255)                             aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCACCGC‑ATGGCGGCAGCGCGCCAGGCGCGCTTCATAGGCTTTCCCTTCACCATGCCGCGTTTTGTGTTcccagagggcggcgccc                                                                                                                                                                                                     >  2:1088110/1‑223 (MQ=255)                               cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCCCTTCCCGATGCAGCGTTTGCTGTTCCCCGGGATCGGGCccccc                                                                                                                                                                                                   >  1:368142/1‑237 (MQ=255)                               cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCcg                                                                                                                                                                                                   >  1:922611/1‑238 (MQ=255)                               cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCCTGCCAGGCGCGCTTAATAGGCTTCCACTTCACGATGCAGCGTTTTCTGTTCCCCGAGATCGGTAAccca                                                                                                                                                                                                   >  1:277166/1‑237 (MQ=255)                               cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGAGGCAGCGGGCCAGGCGCGCTTCATAGGCTTCCCCTTCACGATGCACCGTTTGCTGTTCCCCGGGATCGGTAAcccc                                                                                                                                                                                                   >  1:402693/1‑237 (MQ=255)                                gcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCCGTTTTCGATTTTTT‑CCAGCCC‑ATGGCGGCAGCGTGCCCTGCCCGCTTCCTAGGCTTCCACTTCCCCCTGCCGCGTTTGCCGTTccccccgatccggccccccc                                                                                                                                                                                                  >  2:584687/1‑220 (MQ=255)                                     ccgcccGGTTCTGGCATGGTTTTTATTTTCCCCCAGGATCCCAATCAAATCGACAGCCAGTTTGACATCGGCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa                                                                                                                                                                                            <  2:231644/234‑1 (MQ=255)                                           ggTTCTGGGGGGGATCTGATTTTCCTCCAGGAGCAAAATAAAAGCGACATCCAGTTTGACCTCGTGAGGGTGATTTTCCAGTGACATATTCTCTCCATTGCTTGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGct                                                                                                                                                                                      <  2:922611/239‑1 (MQ=255)                                                tgcgggggttttGATTTTCCGCCAGCAGCACAATCAAATCGACAGCCAGTTTTACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑g                                                                                                                                                                                <  2:402693/228‑1 (MQ=255)                                                tGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGGGATCGGTCACCCGCCCCAGACGGGCCCTGC‑g                                                                                                                                                                                >  1:628110/1‑239 (MQ=255)                                                tGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGCTCGGTCACCCGCGCCCGACGGGCTCTGC‑g                                                                                                                                                                                >  1:630224/1‑239 (MQ=255)                                                tGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑g                                                                                                                                                                                >  1:1081507/1‑239 (MQ=255)                                                  gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTcc                                                                                                                                                                                                                                         >  1:152358/1‑180 (MQ=255)                                                  gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                                                                                                                                                                                                         <  2:152358/181‑1 (MQ=255)                                                  gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGGGATCGGTCACCCGCGCCAGACGGGCTCTGC‑Gt                                                                                                                                                                               >  1:705153/1‑238 (MQ=255)                                                       gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGCTTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGGCACCCGCGCCAGACGGCCCCTGC‑CTTCGGCa                                                                                                                                                                         >  1:298546/1‑239 (MQ=255)                                                       gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCCGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCCCTTCACCATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCCCCAGACGGGCTCTGC‑GTTCGGc                                                                                                                                                                          >  1:1078531/1‑238 (MQ=255)                                                        aTCTGATTTTCCCCCAGCAACCCAATCAAAACGACAGCCAGTTTGACATCGGCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCa                                                                                                                                                                         <  2:705153/238‑1 (MQ=255)                                                         tCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCaa                                                                                                                                                                        <  1:668105/238‑1 (MQ=255)                                                            ggttttCCCCCAGCAGCACAATCAAATCGACAGGCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCAt                                                                                                                                                                    <  1:632724/237‑1 (MQ=255)                                                            gATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCAt                                                                                                                                                                    <  1:569123/239‑1 (MQ=255)                                                                  cctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACg                                                                                                                                                              >  1:154401/1‑239 (MQ=255)                                                                        acagccaaaacAAACCGACCGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTACCCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCCCTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAgccg                                                                                                                                                        <  1:1088110/229‑1 (MQ=255)                                                                            cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCGGTTCCACGTGCTCGGTCACCCGCGCCAGCCGGG‑TCTTCGGGTCGGCAACCacttcccttaccccccg                                                                                                                                                     >  1:473989/1‑222 (MQ=255)                                                                                aacAAAGCCACAGCCATTTTCACATCGTCTGGGGCATTTCCCAGTGACATATTCTCTCCATTGCGAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑AGGCCGGCAGCGTGCCAGGCGCGCTTCAAAGGCTTCCTCTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCaa                                                                                                                                                <  2:202172/237‑1 (MQ=255)                                                                                        gccaccccttttGTCCTCGTCAGGGGCATTTTCCAGTGAATTTTTCTCTCCCTTGCTGGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTCCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGa                                                                                                                                        <  2:642664/230‑1 (MQ=255)                                                                                           aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGCCACCCGCACCAGACGGGGTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCCCTCAAAACCCTGATGc                                                                                                                                     >  1:723248/1‑239 (MQ=255)                                                                                                           tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCCCGAGATCGGGCACCCGCGCCAGACGGGCCCTGC‑GTTCGGCAACCACCCCACGTAACCGCCCCCCAAAATCCTGATgttcaaaaactttacccg                                                                                                                     >  2:1073687/1‑222 (MQ=255)                                                                                                           tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGc                                                                                                                      >  1:788844/1‑238 (MQ=255)                                                                                                             aGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGcgt                                                                                                                    >  1:1064159/1‑236 (MQ=255)                                                                                                                 cctttttccggggtctttttCTCTCCTTGGCCGGGGGGTT‑AACCGGCCTACCCTGTTTTGGATTTTTT‑CCGGGGC‑ATGGCGGCCGGCTCCCGGGCGCGCTTCATGGGCTTCCCCTTCAGGATGCCGCGTTTGCTGTTCCACGAGATCGGTCCCCCGCCCCAGACGGGCTCTGC‑TTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAg                                                                                                                <  2:630224/220‑1 (MQ=255)                                                                                                                           tgtcttttttttctcCTTTTCCAGGGGGTT‑AAACGCCCTACCTTTTTTTCGATTTTTT‑CCCGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTCCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAAtttt                                                                                                     <  2:20616/228‑1 (MQ=255)                                                                                                                             gaaattttCCCTCCATTGCTAGGGGGTT‑AAACGGGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑AGGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAAtttt                                                                                                     <  1:584687/231‑1 (MQ=255)                                                                                                                              aCATTTTCCCCCCATTGC‑AGGGGGTTCAAGCGCGCTACCCTTGTCTTGATTTTTA‑CCGGCGC‑TTGGCGGCAGCGTGCCGGGCGCGCTACACAGGCTCCCCCTGCACGCTGCGGCGTTTGGTTTTCCACGAGATCGGTCACCCGCGCCAGCCGGGCTCTGA‑GTTCGGCAACCTTCTCACGGAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCgg                                                                                                  <  2:823068/239‑1 (MQ=255)                                                                                                                                   ttCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCCGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCggtggt                                                                                              >  1:706946/1‑238 (MQ=255)                                                                                                                                      tctcCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCACAAAATCCTTATGCTAAAAACGTTTACGCGCCCAAGGGGCAATTTTCGGGGGTGccc                                                                                          >  2:624168/1‑237 (MQ=255)                                                                                                                                        tcCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATCCGTTTCACCCGCCAGCGGGGAATTTTCGGGGGGCCACt                                                                                         >  2:681790/1‑238 (MQ=255)                                                                                                                                        tcCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCa                                                                                           >  1:20616/1‑236 (MQ=255)                                                                                                                                        tcCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACt                                                                                         >  1:734393/1‑238 (MQ=255)                                                                                                                                        tcCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGGAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCACCGGGCAATTTTCGTTGGTGCACt                                                                                         >  1:43445/1‑238 (MQ=255)                                                                                                                                             tGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑TTGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCACAATCCTGATGCTGAATACGTTTACCCTGCCACAGGGCAATTTTCGGTGGTGCACTTTccc                                                                                    >  1:522996/1‑238 (MQ=255)                                                                                                                                             tGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATCCGTTTACGCTGCCAGCGGGCAATTTTCGGTGGGGCACTGTccc                                                                                    >  1:114721/1‑238 (MQ=255)                                                                                                                                             tGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGACCTGTccc                                                                                    >  1:1078534/1‑238 (MQ=255)                                                                                                                                               ctggCGGGTT‑AACCCCTCTACCTTTTTTTTCATTTTTT‑‑CAGGGCGAGGGGGGCGGCGGCCCAGGCGCCCTTCAGAGGCTCCCTCTGCACGATGCAGGGGTGGGTGTTCCCCGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCCCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACt                                                                                 <  2:528062/236‑1 (MQ=255)                                                                                                                                               ccggggggTT‑AAACGCGGTACCCTGTTGTCGATTTTTT‑CCAGCGA‑CTGGCGGCAGCGTGCCAGGCGCGCTTCAAAGGCTTCCACTTCCCGATGCAGCGTTTGCTTTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACt                                                                                 <  1:425382/234‑1 (MQ=255)                                                                                                                                                tggCGGGTT‑AAACCCGCTAACCTTTTTGCGTTTTTTC‑CCAGCGC‑ATGGCGGCCGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGGTGTTCCACGAGATCGGTCCCCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTc                                                                                <  2:788844/237‑1 (MQ=255)                                                                                                                                                  gCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCCAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTcc                                                                               >  1:481781/1‑237 (MQ=255)                                                                                                                                                    gggTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑g                                                                                                                                                                                <  1:749047/139‑1 (MQ=255)                                                                                                                                                    gggTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑g                                                                                                                                                                                >  2:749047/1‑139 (MQ=255)                                                                                                                                                                 cTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAAAACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCg                                                                >  1:138333/1‑239 (MQ=255)                                                                                                                                                                    aCCTTTTTTTGTTTTTTTCCCAGCGC‑GGGGGGGCGGGGTGCCGGGGCCGCTTCAAGGGTTTCCCCGTCCGGATGCAGGTTTTGCTGTTCCAGGAGATCGGCCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGa                                                              <  2:137619/239‑1 (MQ=255)                                                                                                                                                                          tttCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGGGGCTTc                                                        >  1:32403/1‑238 (MQ=255)                                                                                                                                                                              gATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACCCTGCCCGCGGGCCATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGCCCAGCCGGAGGCTTCCcgc                                                    >  1:995494/1‑238 (MQ=255)                                                                                                                                                                                   ttt‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGGGGTGCCCTGGCCCACTCCTCCAATACCCAGGCGgggggctccagccgaatg                                              >  2:815730/1‑225 (MQ=255)                                                                                                                                                                                           cgc‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCCGGCAATGACCAGGCGGAGGCTTCACGCGCGATGGCTTCCa                                       >  1:484912/1‑239 (MQ=255)                                                                                                                                                                                                  gccgcAGCGTGCCGGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCCCGAGATCGGTCCCCCGCGCCAGACGGGCCCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGAGGCTTCACGCGCGATGGCTTCCAGTtgt                                  <  1:924877/235‑1 (MQ=255)                                                                                                                                                                                                    ggccgggggccgggccggcTCCATGGGCTCCCCCTTCACGATGCAGCGTTTGCTGTCCCCCGAGATCGGCCACCCGCGCCAGACGGGCTCGGC‑GTTCGGCACCCATCTCCCGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGAGGCTTCACGCGCGATGGCTTCCAGTTGTgcc                               <  2:91723/222‑1 (MQ=255)                                                                                                                                                                                                      cAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGt                                                                                                                            >  1:169117/1‑144 (MQ=255)                                                                                                                                                                                                      cAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGGGCACTGTCCCACTCCCGCAATAACCAGGCGGAGGCTTCACCGCCGATGGCTTCCAGTTGGgccg                              >  1:1035892/1‑238 (MQ=255)                                                                                                                                                                                                             ccAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGGAGCCGCCGCCCAAAATCCTGATGCTGAATACGTTTAAGCGCCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCCCCCGAGCAATGACCAGGCGGAGGCTTCACCCGCGACGGCTGCCAGTtggtgcacccaatg                       >  2:256892/1‑226 (MQ=255)                                                                                                                                                                                                                                 tCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGAGGCTTCACGCGCGATGGCCTCCAGTTGTGCCGCCCAATGTTCCCCCACCCAGGCCCCttc  >  2:126043/1‑238 (MQ=255)                                                                                                                                                                                                                                 tCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATCACCAGGCGGAGGCTTCACGCGCGATGGCTTCCAGTTGGGCCCCCCGTCGTTCCGCCACCCCGCCCACtgc  >  2:148590/1‑237 (MQ=255)                                                                                                                                                                                                                                   cACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGAGGCTTCACGCGCGATGGCTTCCAGTtgt                                  >  2:411958/1‑205 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             CTTTTCATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTT‑AAACGCGCTAACCTGTTTTCGATTTTTT‑CCAGCGC‑ATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGGCTCTGC‑GTTCGGCAACCATCTCACGTAGCCGCCGCTCAAAATCCTGATGCTGAATACGTTTACGCTGCCAGCGGGCAATTTTCGGTGGTGCACTGTCCCACTCGCGCAATGACCAGGCGGAGGCTTCACGCGCGATGGCTTCCAGTTGTGCCGCCAGATGTTCCGCCAGCCAGGCCACTTG  >  NC_000913/490143‑490602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑