| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 559,176 | T→A | 17.8% | V68E (GTA→GAA) | sfmC → | putative periplasmic pilus chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 559,176 | 0 | T | A | 17.8% | 118.4 / 6.0 | 52 | V68E (GTA→GAA) | sfmC | putative periplasmic pilus chaperone |
| Reads supporting (aligned to +/- strand): ref base T (36/6); new base A (2/8); total (38/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGTCAGGAATACACAAGGCGTATTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGAA > NC_000913/558945‑559414 | ccGTCAAGAATTCACCAGGCTTTTTAACTATTATGACTAAAATAAAGTTAGTGATTCTCATTTTATTTTATTTAATCATTTCGGCCAGCGCCCAAGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑Taaaagaa < 2:878277/239‑1 (MQ=255) aTACCCAAGGCGTTTTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGtcatt < 2:642786/238‑1 (MQ=255) caAGGCGTATTAACTATGATGACTAAAATAAAGTTATTGATTCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTcatcat < 1:935376/239‑1 (MQ=255) aTTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGtcatt < 1:234665/225‑1 (MQ=255) atgaCTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAACTAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGGG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACtgtttgt > 1:510862/1‑239 (MQ=255) atgaCTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATTAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACACAAGCGGGG‑TAAAAGAAAAGTCATTCATCATTACAcctgtctctaaaaa > 2:234665/1‑227 (MQ=255) tAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGCATGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTAAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TCAAAGAAAAGcaattca > 1:337849/1‑212 (MQ=255) aaTAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGCCTGCGGTATTGATTAGACATAGCCATACCAATGAGCGCTTTCTGGCCAA‑TTCGTGGATTGAAAACAGCAGCGCTG‑TAAAAGAAAAGTCATTCCTCATTACCCCGCCACTGTTTGTTAGAGaa > 2:218412/1‑238 (MQ=255) aGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTAATTAATCATTACACCGCCACTGTTTGTTAGGAAACCCaaa > 2:580641/1‑239 (MQ=255) tatttaATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGtattat > 1:512416/1‑239 (MQ=255) aaTCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAATCGAAAAAACTTTGCGAATTATTTac > 1:1012316/1‑238 (MQ=255) tCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTAGACCGCCACTGTTTGTTAGTGAACACAAAAACGAAAAAAATTTACTTATAATTTACAcc > 2:462499/1‑239 (MQ=255) tCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCCCCACCGTTGTTTAGTGAACCCAAAAACAAAAAAACATTCGGAATTATTTAAAcc > 2:422718/1‑239 (MQ=255) tCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACAGCACCGTTTTGTTAGTGAACCCAAAAGCAAAAATACTTTGCGCATTATTTAAACAGGTcc > 2:396844/1‑239 (MQ=255) cccccccctggctgcGGGAGGTGTCGTATGGGGTGCCGGGTTTATTATTTCCCCCCATGATGAAAACCATGCGGCGGTATGGATTAAAAATAGCCATCCAAAGGAGCCTTTTCTGGTAAA‑TTCGTGGATTGAAAACAGCAGCGGTA‑AAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGc < 2:541189/229‑1 (MQ=255) aTGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTGAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCCAGTTCTGGTCAA‑TTCGAGGATTGAAAACAGCAGCGGTG‑AAAAAGAAAAGTCATTCATCATTACACCTCAACTGTTTGTTAGTGAACCCAAAAGCGAAAAACCTTTGGGAATACTTTAAACAGGCCCACCCCGGgaagca > 2:549988/1‑239 (MQ=255) aTGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TACAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCCCTGgcagca > 1:685708/1‑239 (MQ=255) aTGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATCATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGGGAAAATACTTTGCGaattat > 1:723445/1‑214 (MQ=255) cGGAGGCACCGCATGAGGGACCACGCTTATTATTTACCCCGCTGATGCAAAACAGACTGCGGTAGGGATTAGAAATAGCAATACAAATGAGCGCTTTCTGGACAA‑TTCGGGGATTGAAAACAGCAGCGGTT‑AAAAAAAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTg < 1:218412/239‑1 (MQ=255) aTCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCACCGGGG‑ACAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCAAAAAGCGAAAATACTTTTGGTATTTTTTAAACCGGTCCCCCGCTGgcagcaactccaggatc > 2:946343/1‑225 (MQ=255) cGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTAAACCGGTCCCCCCCTGgcaagaaaacggagggtcttgt > 2:751638/1‑220 (MQ=255) ggTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATCCTTGGCGTATTATTTAAACCGGTCCACCCCTGGCAGCAGATCGGGAGTTTTTGTTTTGGAt > 2:581827/1‑239 (MQ=255) gTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTTGGTATTATTTACACCGGTCCACCGCGGGCGGCCAACCGTGAGTCTCTGTTTTGGGTg > 2:23258/1‑239 (MQ=255) ggggttttttttacCCCGTTGTTGCTAAACAGATTGCGGTATGGATTAAAAAAAGCCATACAAATGGGCGTTTTCTGTTCAA‑TTCGGGGATTGAAAACAGCAGCGGTG‑AAAAAGAAAAGTCATCAATATTTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGtt < 1:422718/230‑1 (MQ=255) attattTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAgtc > 1:539917/1‑237 (MQ=255) tatCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTTAACCCAAAAGCGAAAATACTTTGCGACTTATTTACACCGGTCACCCGCTGGCGGAAGATCGTGGGTTCCTGTTCTGGATGAATGTTAAAAGGATc > 2:663237/1‑237 (MQ=255) atCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCt > 1:721949/1‑239 (MQ=255) atCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCt > 2:310216/1‑239 (MQ=255) cccGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTc > 1:176396/1‑239 (MQ=255) cTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTa > 1:1059627/1‑239 (MQ=255) tGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAg > 1:288935/1‑239 (MQ=255) aaaaCAGACGGGGGTATGGATTAAAAATAGCCATCCCAATGAGCGTTTTCTGGTCAA‑TGCGTGGATTAAAAACAGCAGCGTTG‑TAAAAAAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATaaaa < 1:23258/238‑1 (MQ=255) gACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGGATTATTTACACCGGTCCACCGCGTGCAGAAGATCGGGAGTCTATGTTCTGGATGAATGTTAAAACGATCCCTGCGGGAGATAAAAAAGCAt > 2:474820/1‑239 (MQ=255) tGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTAAACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTTTGGCTGAATGTTAAGACGACCCCTCCGGGAGATAAAAATGCAttaa > 2:563696/1‑237 (MQ=255) tttgtataaaaaaTAGCCAACCAAATGGGTGTTTTTGTGTAAA‑TTCGGGAATTAAAACCAGAAGGGGTA‑AAAAAAAAAGTTCATTCACCATTACCCCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACgg < 2:288935/228‑1 (MQ=255) tttGGATTAAAAAGAGCCAAACAATGGAGCGCTTTCT‑GTCAATTTCGTGGATTGAAAACGGCAGGGGTG‑AAAAAAAAAAGTAATACATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACgg < 1:751638/236‑1 (MQ=255) aTTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGaa > 1:997208/1‑239 (MQ=255) aTTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCAACCGCTGGCAGCAGATCGTGAGTCTCTATTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGAATTGAAAGGCCGGaa > 1:389613/1‑239 (MQ=255) aGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCa > 1:1015822/1‑239 (MQ=255) gCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTGGATAAAAATGCATTGAACGACAGGAATGTTTTGCaa > 1:104855/1‑239 (MQ=255) gCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCAACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGAACCCTTCGGTCGATAAAAATGCATTGAACGGCAGGGAATTTTTGCaa > 2:154567/1‑239 (MQ=255) tGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGTTAGGTCAAAATGCATTGAACGGCAGGAATGTTTTGCAAAGGGCGAttt > 2:657496/1‑239 (MQ=255) cgcTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAAGGCATTTAACGGCAGGAATGTTTTGCAAATGGGGATTTTATc > 2:395473/1‑239 (MQ=255) tttCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGAATTGAACGGCAGGAAATTTTTGCAACTGGGGTTTTTATcccc > 2:575809/1‑236 (MQ=255) ttgttgttaattccggggATGGAAAACGGCAG‑GGTGAAAAAAGAAAAGTCATTCATCATTCCACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATcgcg < 1:154567/223‑1 (MQ=255) tgtggaaaa‑TTTGTGCATGAAAAACATCAGGGGTA‑AAAAAAAAAAGTCATTCATCTTTCCTCCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCa < 1:563696/232‑1 (MQ=255) gttttttttgtgtATTAAAAACAGCGGGGGTG‑AAAAAAAAAATGTAGTCACCATCACCCCGCCCCTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGa < 2:510862/230‑1 (MQ=255) gTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGaa > 2:876431/1‑239 (MQ=255) a‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGCAAGGAATGTTTTGCAACTGGCGATTTTACCGCGCATGAAatta > 1:121291/1‑239 (MQ=255) ggATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGCTCCCTTCGGTAGATAAAACTCCATTGAACGGCAGGAATGTTTTGCAACTGGGCATTTTAACGCGCATGAAATTAtttat > 1:439891/1‑236 (MQ=255) cGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAAc > 2:353194/1‑172 (MQ=255) cGGTG‑TAAAAAAAAATTCATTCACCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAAc < 1:353194/172‑1 (MQ=255) tAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGaa > 1:665045/1‑239 (MQ=255) | CCGTCAGGAATACACAAGGCGTATTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTG‑TAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGAA > NC_000913/558945‑559414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |