BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,024,846	T→C	14.9%	S126P (TCT→CCT)	helD →	DNA helicase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,024,846	0	T	C	14.9%	232.5 / 2.8	112	S126P (TCT→CCT)	helD	DNA helicase IV
Reads supporting (aligned to +/- strand): ref base T (34/52); new base C (17/4); total (52/60)
Fisher's exact test for biased strand distribution p-value = 1.14e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCACCGCTGAATCCGGCGCAGGCCCGGGCA  >  NC_000913/1024620‑1025082

cgGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGGGGAGTGGCGAGGTGAGCGAAATTTGGTCTGGGGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAAAAAAT‑GGCTG‑G‑CGCGGGGGCAAACCCCTggggggcggc                                                                                                                                                                                                                                  >  2:889446/1‑231 (MQ=255)

  gTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑AAT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTTGCGTGCAGcaa                                                                                                                                                                                                                                >  1:247136/1‑239 (MQ=255)

     tGGTATGGGGGGGGGTGGAATTTGTACTGCCGGACGAAAAAGGTGGGCGTTCGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGcaacaa                                                                                                                                                                                                                             <  2:356123/239‑1 (MQ=255)

           ggggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGGGGTGCGTCTGCCCGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGc                                                                                                                                                                                                                       <  1:1088130/239‑1 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGGGTGGCGCGATGAGCGAAATTGGGTCTGGTGGTTTACGCCAGCAACCGGATTTGATGTCCACCCGC‑ACT‑GGGG‑GAAAAAAAT‑GGCTGCC‑CGCG‑GGGCAAACCTCCgggtgggaggcaaaaaaccccca                                                                                                                                                                                                                     >  2:1068543/1‑218 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGGGGGGGGAGGTGGGCGAAATTTCGTCTGGGGTTTTACGCCAGCAACTGGGTTTTGTTGCCCACCGC‑CCT‑GGGG‑AAAAAAAAT‑GGGTG‑A‑CGGGTGGGCAAACCCCTGGCGTGCAGCAAAAAAACAGccg                                                                                                                                                                                                                     >  2:881923/1‑238 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGGGCGAAATTGCGTCTGGGGTTTTTCGCCAGCAACTGGATTTGATTGCCACCGGC‑CCT‑GGGG‑AAAATAAAT‑GGCTG‑A‑AGCGGGAGCAAAACCCTGGCGGGCAGAAACAAAACCCCCa                                                                                                                                                                                                                     >  2:811078/1‑239 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGGAATTGCGTCTGGTGTTTTACGCCACCAACTGGGTTTGATTGCCCAGCGC‑ACT‑GGGG‑GAAATAAATGGGCTG‑‑‑CGCGGGCGCAAACCCCTGGCCTgccacacaaaacacgccc                                                                                                                                                                                                                     >  2:210897/1‑223 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGATG‑A‑CGCGTGAGCCAACCCCTGGCGTGCCGCAACAAATCCCCCa                                                                                                                                                                                                                     >  1:128368/1‑239 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGGGTTTTTCGCCCGCAACTTGGTTTGATTGCCACCCGC‑ACT‑TGGG‑GAAATAAAA‑GGCTT‑G‑CGCGTGAGCAAACCCCCGGCGGGCAGGGAAAAAACCGCCa                                                                                                                                                                                                                     >  2:719332/1‑239 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGGGGCGAGGTGGGGGAAATTGGGTCTGGGGTTTTACGCCCGCAACGGGGTTTAATTGCCCCCGGC‑CCT‑GGGG‑AAAAAAAAT‑GGCTG‑ACCGGGTGCGAAAACCT‑TGGGGGGCGCCAAAAAAACCGCCa                                                                                                                                                                                                                     >  2:258742/1‑239 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGGGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACCTGATTTGATTGGCAC‑CGCGACC‑GGGG‑GAAAAAAAA‑GGCTG‑C‑CGAGTGAGCAAAACCCCGGCGTGCCCCAAAAAAACcccc                                                                                                                                                                                                                      >  2:62391/1‑238 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGGGGCGAGAAGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGTCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAAACTCTGGCTGGCTGCAACAAATACGccc                                                                                                                                                                                                                     >  1:403672/1‑238 (MQ=255)

             ggCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGGGGAGTGGCGAGCTGAGCGAAATTGCGTCTGGCGTTTTACGCCAGGAACTGGATTTGCTTGCCACGCGC‑CCT‑GGGG‑AAAAATAAT‑GGCTG‑A‑CGCGGGAGGAAACCCCTGGGGCGCAGCCAaaaaaaccccc                                                                                                                                                                                                                     >  2:870298/1‑232 (MQ=255)

                 aGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGGTGAGCGAAATTGCGTCTGGTGGTTTACGCCAGCAACGGGATTTGATTGCCACGCGC‑ACT‑GGGG‑GCAAAAAAT‑GGCTG‑C‑CGCGTGGGCAAAACCCCGGCGGGCAGCAACAACACCCCAAGGCt                                                                                                                                                                                                                 >  2:980490/1‑239 (MQ=255)

                 aGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGGGCAAACCTCTTGCGGGCAGCAACAAATCCGCCAGCCt                                                                                                                                                                                                                 >  1:11425/1‑239 (MQ=255)

                 aGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGGGGCGAGGTGAGCGAAATTGCGTCCGGGGTTTTACGGCCGCAACTGGGTTTTGTTGCCACGCGC‑ACC‑GGGG‑AAAATAAAT‑GGGTG‑A‑CGCGTGCGCAAACCCCTCGCGGCCAGCAAAAAATCCCCCAGGcg                                                                                                                                                                                                                 >  2:252482/1‑238 (MQ=255)

                 aGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGCGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGGGCAGCAACCAATCCCCCCGGCt                                                                                                                                                                                                                 >  1:695272/1‑239 (MQ=255)

                          ttGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGcg                                                                                                                                                                                                        <  1:645151/239‑1 (MQ=255)

                           tGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGcgt                                                                                                                                                                                                       <  1:644298/239‑1 (MQ=255)

                           tGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGGGTTTTAAGCCAGCAACTGGATTTGTTTGCCACCGGC‑CCT‑GGGG‑AAAATAACT‑GGCTG‑A‑CGCGTGAGCAAACCCTTGGCGTGCAGCAACAAATCACCCAGGCTTTTTCGGcgt                                                                                                                                                                                                       >  1:536660/1‑239 (MQ=255)

                                                ggtgCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGGGCAAACCCCTGGCGTGCAGCCACAAATCCGCCAGGCTTTGCCGGCGTTGCCGTTTCCGGTTAACCgcc                                                                                                                                                                                  >  1:809199/1‑237 (MQ=255)

                                                    cGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCCGGGGTTTTACGCCAGCAACTGGATTTGATTGCACCCCGC‑ACT‑GGGG‑AAAATAAAA‑TGCTG‑A‑CCGGGGGGCAAACCTCTGGCGTGAAGGAACAAATCCGACAGGCTTTTTCGGCGGTGCCGTTGCCGGTGAACAGACAGga                                                                                                                                                                              >  2:41151/1‑239 (MQ=255)

                                                     gtttGCACGGAACCGAATGGGGGGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTgg                                                                                                                                                                               <  1:491423/234‑1 (MQ=255)

                                                       cggCACGGCACCGAATGGGGCGAGACGCAGCGTGTTTACCATCATCTTGATGCTCACTGGCGGCTGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGgaag                                                                                                                                                                            <  1:41151/236‑1 (MQ=255)

                                                       cTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCCGCAACTGGGTTTGATTGCCACGGGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGGGGGCAAACCCCTGGCGTGCAGCAACAAATCCGCCCGGCTTTTTCGGCGTTGCCCTTCGCGGTTAACCGACCGgaaaa                                                                                                                                                                           >  2:1067926/1‑237 (MQ=255)

                                                         gccgggCACCGAAGGGGGCGAGACGGAGCGTTTTTGCCATCATGTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAAt                                                                                                                                                                         <  1:476217/235‑1 (MQ=255)

                                                           acgggtccGAAGGGGGCGAGACGGAGGGGTTTTACCATCATCTTGATGCCCCCTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAACCCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTc                                                                                                                                                                       <  1:171188/233‑1 (MQ=255)

                                                               cACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGGGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGcgttg                                                                                                                                                                                                     <  1:537951/205‑1 (MQ=255)

                                                               cACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGt                                                                                                                                                                                          <  1:795486/216‑1 (MQ=255)

                                                               cACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGGGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑CCT‑GGGG‑AAAATAAAT‑GGCTG‑C‑CGCGGGGGCAACCCTCTCGCGTGCAGCAACAAATCCGCCCGGCTTTGTCGGCGGTGCCGTGCCCGGGTAACGCACGGgaaaaaattaa                                                                                                                                                                     >  2:491423/1‑232 (MQ=255)

                                                               cACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCGCACTGGCGGCGGTGGGGTGGCCAGATCAGCGAAATTGCGGCTGGGGGTTTACGCCACCACATGGATTTGATAGCCGCGCGC‑ACT‑GGGG‑GTAATAAAG‑GTACA‑A‑CGGTTGCGGAAACCTCCTGCTCGCAGGACCAAAAACGCCTCGCATTTCCGGCCGGGCCGTTCCCGGTTAAcccgacggaaaaatcacaaa                                                                                                                                                                   >  2:955726/1‑221 (MQ=255)

                                                                 ccGAATGGGGGGAGCCGCAGCGTTTTTACCATCATCTTGATGCTCCCTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATaa                                                                                                                                                                  <  1:281097/238‑1 (MQ=255)

                                                                   gAATGGGGGGAGACGCGGGGTTTTTACCATCTTCTGTATGCTCCCTGGCGGCGGTGGAGTGGCGAGATGAGGGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTg                                                                                                                                                               <  2:872120/239‑1 (MQ=255)

                                                                   gAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTg                                                                                                                                                               <  1:258742/239‑1 (MQ=255)

                                                                         ggCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGGGCAAACCTCTGGCGTGCCGCCACAAAACCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCCCCTggcggtcccttttccacatctgac                                                                                                                                                         >  2:795486/1‑217 (MQ=255)

                                                                         ggCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCCGGTGTTTTACGCCCGCAACTGGATTTGATTGCCACGAGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CCCGTGAGCAAAACACTGGCGGCCAGCAAAAAATCCGCCAGGCTTTCTGGGCGTTGCCGTTTACGGGTAACCGCCGGgaaaaaaccagtaatgcccgcgg                                                                                                                                                         >  2:116104/1‑222 (MQ=255)

                                                                           cGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCCACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCGG‑A‑CGCGTGAGCAAACCCCTGGCGGGCAGCAACAAATCAGCCCGGCTTTGTCGGGGGTGCCGTTGCCGGTTAACCCACTGGAAGAATTCGAAAAccgccgcgggg                                                                                                                                                       >  2:32802/1‑234 (MQ=255)

                                                                              gaCGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCCAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTTTCGGGGTTGCCGTTTCCCGTTAACCGCCTGGAAGAATTCGGTaaacgcccggaggggt                                                                                                                                                    >  2:713936/1‑225 (MQ=255)

                                                                              gaCGCAGCGTTTTTACCATCATCTTGAGGGTCACGGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACGGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAggcg                                                                                                                                                     <  1:1068543/238‑1 (MQ=255)

                                                                              aacggggCGTTTTTTCCATCCTCTTGATGCTCACGGCGGGCGGGGGAGTGGCGAGATGAGCGAAAGTGCGGCTGGGGTTTTACGCCGGCAACTGGATTTGATGGCCACGCGG‑ACT‑GGGG‑AAAACAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATACCTGCCGTGAggcg                                                                                                                                                     <  2:757599/232‑1 (MQ=255)

                                                                                   aGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCCACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGCATTCGATAACTGCCGTTAggcgtggcgt                                                                                                                                               >  1:675655/1‑239 (MQ=255)

                                                                                    ttttataggggatgcttttGGTGCCCACGGGCGGGGGTGGGGGGGGGAGATGGGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACG‑GGGA‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAACCCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTa                                                                                                                                              <  2:2004/222‑1 (MQ=255)

                                                                                      gTTTTTACCATCATCTTGATGCTCCCTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAggcgtggcgt                                                                                                                                               <  1:713936/236‑1 (MQ=255)

                                                                                              catcatCTTGTTGCTCAGTGGGGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCGGGTGTTTTACGCCAGCAACGGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGc                                                                                                                                    <  2:140430/239‑1 (MQ=255)

                                                                                              catcatCTTGATGCTCCCTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTTTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGc                                                                                                                                    <  1:673816/239‑1 (MQ=255)

                                                                                              catcatCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGc                                                                                                                                    <  1:870298/239‑1 (MQ=255)

                                                                                              catcatCTTGATGCCTACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGc                                                                                                                                    <  1:812507/239‑1 (MQ=255)

                                                                                               atcatcTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCCGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTTCCGGTTAACCCGCTGGAAGAATTCGAGAACAGCCGGGAGGCGGGGGGGAAATGTCAGGcc                                                                                                                                   >  2:644448/1‑239 (MQ=255)

                                                                                                 catcTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTTCCGTTGCCGGTTAACCGACTGGGAGAATTCGCTAACTGCCGTGAGGCTCGGCGTAAATATCAGGCCTg                                                                                                                                 >  1:655507/1‑239 (MQ=255)

                                                                                                   tcTTGATGCCCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGc                                                                                                                                    <  1:455079/234‑1 (MQ=255)

                                                                                                        atggcgcggggggggggTGGAGGGGGGGGAGGGGCGAAATTGCGTCGGGTTTTTTCCGCCAGCAACTGGATTTGATTGCCACGGGC‑ATG‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGaaa                                                                                                                          <  2:20230/224‑1 (MQ=255)

                                                                                                         tgcgccgggggcgggggggggTGGCGAGATGAGGGAAATTGCGTTTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCGCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCTGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGaaa                                                                                                                          <  2:752058/218‑1 (MQ=255)

                                                                                                             cACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑GAAATAAAT‑GGCTG‑A‑CGCGGGAGCAAACCTCTGGCGTGCAGCAACAAAACCGCCAGGCTTTGTCGGCGTTGCCCTTTCCGGTTAACCCACTGGCAGAATTCTGAAACAGCCGGGAGGCGGGGCGTAAAAGTCCGGCCTGGTTGaaaacgg                                                                                                                      >  2:455079/1‑234 (MQ=255)

                                                                                                              aCTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGc                                                                                                                                    >  1:2004/1‑223 (MQ=255)

                                                                                                               cTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTCACGTGAGCCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTg                                                                                                                   >  1:610985/1‑239 (MQ=255)

                                                                                                                     gcggTGGTGTGGCGAGATGAGCGAAATTGCGTCGGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACG‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCGCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAgcg                                                                                                             <  2:723543/239‑1 (MQ=255)

                                                                                                                      gggggggggggCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAG‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAgcg                                                                                                             <  2:809646/229‑1 (MQ=255)

                                                                                                                       ggTGGGGGGGCGAGATGAGGGAAGTTGCGTCTGGTTTTTTACGCCAGCACCTGGATTTGATTCCCACGCGC‑ATG‑GGGG‑AAAATAAAG‑GGCTG‑A‑CGCGGGAGCAAACCTCTGGCGTGCAGAAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCt                                                                                                           <  2:838317/239‑1 (MQ=255)

                                                                                                                             ggggCGTGGTGAGGGAAATTGCGTCTGGGGTTTTCGGCCAGCCCGTGGATTTGATTCCCCCGCG‑‑ACG‑GGGGAAAAATAAAT‑GGCCG‑G‑CGCGTGAGCAAACCTCTGGCGGGCAGCAACAACTCCGCCAGCCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATGCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGtt                                                                                                      <  2:816704/236‑1 (MQ=255)

                                                                                                                              tggggatgtgggCGAAATGGCGTTTGGGGTTTTACGCCAGCAACTGGATGTGATTGCACAGCGC‑ACT‑GGGG‑AAAATAAAG‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGGCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTg                                                                                                     <  2:247136/227‑1 (MQ=255)

                                                                                                                              tGGCGAGTTGAGCGAAATGGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACG‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTg                                                                                                     <  1:871615/238‑1 (MQ=255)

                                                                                                                                 agacaggAGCGAAATTGCGTCGGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTgcagc                                                                                                 <  1:120312/233‑1 (MQ=255)

                                                                                                                                  gaggtgggggaaagggggttgggTTTTTTACCCCAGAAACTGGTTTGGATTGCCCCGGGC‑CCG‑GGGG‑AAAATAAAG‑GGCTG‑A‑CGCGGGAGCAAACCCCTGGCGGGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTgcagc                                                                                                 <  2:302603/217‑1 (MQ=255)

                                                                                                                                      tggggaaaaTTGCGTCTGGTGTTTTAGGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAAc                                                                                            <  1:32802/233‑1 (MQ=255)

                                                                                                                                      tGAGCGAAATTGCGTCTGGTTTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAAc                                                                                            <  1:719332/239‑1 (MQ=255)

                                                                                                                                      tGAGCGAAATTGCGTCTGGTGTTTTACGCCAGGAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGATCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAAc                                                                                            <  1:64150/239‑1 (MQ=255)

                                                                                                                                      tGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGA‑ATT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAAGTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAAc                                                                                            <  1:617054/239‑1 (MQ=255)

                                                                                                                                       tggccaaatttgggcTGGTGTTTTCACCCCGCAACGGGATTGGATTCCCACGCAC‑ACT‑GGGA‑AAAATAAAT‑GGCGG‑A‑CGCTTGAGCAAACCGTTGGCGTGCAGCAACAAATCCGCAAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATGCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAAc                                                                                            <  2:120167/224‑1 (MQ=255)

                                                                                                                                       gtcgaaaatgtggtttGGTTTTTTACGCCAGCAACGGGATTTGATTGCCACGCGC‑CGT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCCCTGGCGTGCAGCAACAAACCCGCCAGGCTTTGTGGGCGTTGCCGTGGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAAc                                                                                            <  2:45293/223‑1 (MQ=255)

                                                                                                                                             aaTTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCg                                                                                      <  1:210897/238‑1 (MQ=255)

                                                                                                                                               ttGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGtat                                                                                   >  1:136644/1‑239 (MQ=255)

                                                                                                                                               ttGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTGCAAGCTGAACCAGGCGta                                                                                    >  1:846324/1‑238 (MQ=255)

                                                                                                                                                 gggtttGGTTTTTTGCCCCCGCAACTGGATTGGATGGCCCCGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑T‑CGGGTGAGAAACCCCCGGGCGGGCAGCAACAAATCCGCCAGGTTTTGTCGGCGTTGCCGGTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATAc                                                                                 <  2:837122/234‑1 (MQ=255)

                                                                                                                                                  gtcttggtttttgcctcaggaaaCTGGATTTGATTCCCCCGCGC‑ACT‑GGTG‑AAAATAAAT‑GGCGG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTGGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATAcc                                                                                <  2:695272/218‑1 (MQ=255)

                                                                                                                                                  ggtttGGTGTTTTCCGCCAGAAACTGGATTTGATTGCCGCGGGC‑AAG‑GGGG‑AAAATAAAT‑GGCGG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGGCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATAcc                                                                                <  2:809199/235‑1 (MQ=255)

                                                                                                                                                  cGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGGGCCGGGGTGCAGCAAAACCAGGccgtggca                                                                                >  2:120312/1‑232 (MQ=255)

                                                                                                                                                    tCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCCGCCACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTTCCGTGGGGCGTGGCGTAAATTACAGCACTGGTTGAACGATTTTGAAACCGCCCGGTGGCGGCAAAACCCGGCATATAccaa                                                                              >  2:565283/1‑237 (MQ=255)

                                                                                                                                                       ggTTTTTTACGCCAGAAACTGGGTTGGATTGCCCGGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGGGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGc                                                                           <  2:333390/239‑1 (MQ=255)

                                                                                                                                                       ggTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGc                                                                           <  1:854645/239‑1 (MQ=255)

                                                                                                                                                         ttttttTCGCCGGCAACTGGGTTTGATTGCCA‑GCGG‑CCTGGGGG‑AAAATAAAT‑GGCTG‑G‑CGGTGGGGAAAACCTCTGGGGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCa                                                                         <  2:961446/237‑1 (MQ=255)

                                                                                                                                                          tttttCCGCCAGCAACGGGATTTGATTGCCCCGGGC‑ACG‑GGGG‑AAAAAAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCGGGCGTGAAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCa                                                                         <  2:128368/237‑1 (MQ=255)

                                                                                                                                                          gtTTCACGCCAGCAACTGGATTTGATTGCCACGGGC‑ACT‑GGGG‑AAAATAAAG‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGAAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCa                                                                         <  1:1067926/238‑1 (MQ=255)

                                                                                                                                                                     gCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGGGGCGTAAATGTCAGGCCGGGTTGAAAGATATTGAAAGCGCTCGGTTGCCGCATAAACAGGGGATTACCGAACCCATCCATAcccgga                                                             >  2:867841/1‑235 (MQ=255)

                                                                                                                                                                             aTTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGCAAGCCCCCGGTTGCAACATAACCAGGCGTAGACCGAAGCCTAGCTTACCGAGAATGCGGAt                                                     >  2:108444/1‑239 (MQ=255)

                                                                                                                                                                                            gc‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGt                                       <  1:811078/238‑1 (MQ=255)

                                                                                                                                                                                            gc‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGGGCAGCAACAAATCCGCCAGGTTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGt                                       <  1:62391/238‑1 (MQ=255)

                                                                                                                                                                                                             aaat‑GGCTG‑A‑CGCGTGAGCAAACCTTATGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGGCTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCACCGCt                        <  1:46907/239‑1 (MQ=255)

                                                                                                                                                                                                               at‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGGGTATGCGGATTTTTTCCGCCAGGCGCAGTCTTTCACGCTGa                      >  1:3783/1‑239 (MQ=255)

                                                                                                                                                                                                               at‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCCCCGCTGa                      >  1:765812/1‑239 (MQ=255)

                                                                                                                                                                                                               at‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAAGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGACATGCTTACCGCGTATGCGGATTTTTTCCGCCAGGCCGAGTCTTCACCGCTGa                      >  1:312448/1‑239 (MQ=255)

                                                                                                                                                                                                                acggCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGGCGGCGTTGCCGTTGCCGGGTAACCGACTGGAAGAATTCGATAACTGCCGTGAGCCGTGGCGTAAATGGAAGGACTGGGTGAAAGCTATGTAAAGGGCGCGGTTGCAGCATAACCAGGGGTATTCCGGAGAAATGCTTACCGCGAATGAGGCTTTTTTCCGccgaacacgtgctcaccccctga                      >  2:84062/3‑218 (MQ=255)

                                                                                                                                                                                                                        a‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCACCGCTGAATCCgg                >  1:787512/1‑238 (MQ=255)

                                                                                                                                                                                                                          cgggTGAGCAAACTCCGGGCGTGCAGGAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCACCGCTGAATCCGgcg              <  1:889446/236‑1 (MQ=255)

                                                                                                                                                                                                                              tGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGGTAACCGACTGGAAGAATTCGATAACTGCCGGGAGGCGTGGCGTAAATGTCAGGCCTGGTTGACAGATATTGAAAGCGCTCGGGTGCAGCATAACCAGGCGGTTACCGCAGCCATGCTTCCCGGGTAGTCGGTTTTTTTCCGCCAGGCCGAGTTTTCACCTCTGAATCCGGCGCAg           >  2:733562/1‑238 (MQ=255)

                                                                                                                                                                                                                                aGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGc                                                                                                                                    <  2:191573/115‑1 (MQ=255)

                                                                                                                                                                                                                                aGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTg                                                                                                     >  2:1013835/1‑146 (MQ=255)

                                                                                                                                                                                                                                aGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTg                                                                                                     <  1:1013835/146‑1 (MQ=255)

                                                                                                                                                                                                                                     aCCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCACCGCTGAATCCGGCGCAGGCCCGGGc   >  1:924744/1‑239 (MQ=255)

                                                                                                                                                                                                                                      ccTCTGGGGTGCAGTAACAAAGCCGCAAGGCTTTGTGGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCACCGCTGAATCCGGCGCAGGCCCGGGCa  <  1:759237/239‑1 (MQ=255)

CGGTCTGGTATGGGGCGAGCTGGAATTTGTACTGCCGGACGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTTTACCATCATCTTGATGCTCACTGGCGGCGGTGGAGTGGCGAGATGAGCGAAATTGCGTCTGGTGTTTTACGCCAGCAACTGGATTTGATTGCCACGCGC‑ACT‑GGGG‑AAAATAAAT‑GGCTG‑A‑CGCGTGAGCAAACCTCTGGCGTGCAGCAACAAATCCGCCAGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAGGCCTGGTTGAAAGATATTGAAAGCGCTCGGTTGCAGCATAACCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGATTTTTTCCGCCAGGTCGAGTCTTCACCGCTGAATCCGGCGCAGGCCCGGGCA  >  NC_000913/1024620‑1025082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`