Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,901,015	T→C	21.5%	S191G (AGC→GGC)	yoaE ←	putative membrane protein/conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,901,015	0	T	C	21.5%	127.7 / 5.3	62	S191G (AGC→GGC)	yoaE	putative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base T (26/20);  new base C (4/10);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATG  >  NC_000913/1900781‑1901246                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              cggcggtgtgtggAACATTGGCCTGACGTTCCCCGCCAACCAAACGCAGGATGGCATCGGCAGTACGGGCGCGCAGCGGCAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAAGCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                           <  2:174483/227‑1 (MQ=255) cGGCATCGTGCTGAACATGGGCCTGGCGTTGCCCGCCCAACAACCGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTAGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                           <  1:380947/239‑1 (MQ=255) cGGCAGCGTGCTGAACATGGGCCGGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                           <  1:635721/239‑1 (MQ=255)         gtgggaccacgggCCCGGCGTTTCCCGCCCATCAAACGCAGGATGGGATCGGCATGACGCGCGCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGGGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGcac                                                                                                                                                                                                                                  <  2:550655/228‑1 (MQ=255)              aaCATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGGGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGGTGACA‑CCGAAACCTTCTGCCACCCACCACAAACCCATCATTAACAGGAAGCCccgaca‑gaaccacacc                                                                                                                                                                                                                              >  2:793634/1‑230 (MQ=255)               aCATTGGCCTGACGTTTCCCGCCCAGCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAACCCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGcacca‑cca                                                                                                                                                                                                                            <  1:690443/238‑1 (MQ=255)                   tGGCCTGGCTGTTCCCGCCCAGAAAACGCAGGAGGGCATCGGCAGTACGCGCTCGCAGGGGAAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTg                                                                                                                                                                                                                       <  2:82674/239‑1 (MQ=255)                   tGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCCTACA‑GGAAACCTTTCGGA‑ATTTGGAAA‑CCGAAACCTTCTCCCCCCAGCACCACACCCACCCTTAACAGAGAACCCACACA‑GAg‑acca‑ccaaccccg                                                                                                                                                                                                                      >  2:50478/1‑233 (MQ=255)                   tGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACCCAGCCCAACCATTAACAGGAACCCCAAAAA‑GAACACCA‑CCACCCTg                                                                                                                                                                                                                       >  2:1042752/1‑239 (MQ=255)                        tgaactttCCCGCCCATCAAACGGAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGGCGACTGGGGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTg                                                                                                                                                                                                                 <  1:974699/234‑1 (MQ=255)                        tGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCCGCCTCAGACCAATCATTAACAGGAAGCTCAGACA‑GGGCACCA‑CCACCGAGGG‑Gt                                                                                                                                                                                                                  >  1:844227/1‑238 (MQ=255)                        tGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCCGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCCAACCCAAACCAATCATTAAAAAGAAACCCAGACA‑AAGCACCC‑CCACCGTggg‑ggg                                                                                                                                                                                                                 >  2:1046396/1‑237 (MQ=255)                          aCGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCCGACTCAGACCAATCATTAACAGGACGCTCAGACA‑GAGCACCA‑CCACCGCGGG‑Gt                                                                                                                                                                                                                  >  1:807064/1‑236 (MQ=255)                          aCGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAAAAGGAAGCTCAGACA‑GAGACCCA‑CCACCGGGGG‑GGGcg                                                                                                                                                                                                               >  1:928256/1‑238 (MQ=255)                                    cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCACTCATTAAAAGGAAGCTCCGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGGTAACGa                                                                                                                                                                                                      >  1:163340/1‑238 (MQ=255)                                    cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcagaca‑g                                                                                                                                                                                                                                       <  1:76467/207‑1 (MQ=255)                                    cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAAGCCAATCATTAACAGGAAGCTcagaca‑g                                                                                                                                                                                                                                       >  2:76467/1‑207 (MQ=255)                                         cAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGGTGATCGAGAAGCCAATCGCGGCATACA‑GGGAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGAACCA‑CCCCCGTGGG‑GTGCGGGCTAACGAACc‑gcg                                                                                                                                                                                               >  1:667632/1‑239 (MQ=255)                                           aaCGAAGGATGGGATCGGCAGTTAGCGACCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATTGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTc                                                                                                                                                                                             <  1:1083115/239‑1 (MQ=255)                                                     ggCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTgg                                                                                                                                                                                   <  1:284403/239‑1 (MQ=255)                                                          cGGGAGTAGGCGCGCGGAGTGGGAAAGGCCGGTGGTGGCGAACAAAGTGGGGACGCGCAATCTGGTTAAACACTTCGAGGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGcc                                                                                                                                                                              <  2:446597/239‑1 (MQ=255)                                                             cAGTACGCGCTCGCAGGGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAACCCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCTCCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAg                                                                                                                                                                            <  1:793634/238‑1 (MQ=255)                                                                    cggtCGCAGCGGCAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAATTTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACAt                                                                                                                                                                      <  1:1046396/234‑1 (MQ=255)                                                                         ggagcggaaatgtttgttgggggCGAAAAAAGTTCCGACGCCCAATCTGTGAAAACCTTTGGATGCTGACCGAGAAGCCAACCGCGCTAAACA‑GTGACCCTTTCGAA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACCCAGACCAATCATTAACAGGACGCTCAGACA‑GAGCACCA‑CCCCCTTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATTCCAGCAACATAACCGcc                                                                                                                                                               <  2:921072/222‑1 (MQ=255)                                                                              gggaaaGTCGACTGGGGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAAAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATc                                                                                                                                                            <  1:1084446/234‑1 (MQ=255)                                                                                            gtggCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCACCGGTTTGGATGCCAGCACCATAACCCCCATCGCAAtacatacctcc                                                                                                                                             >  1:953057/1‑228 (MQ=255)                                                                                               gcgactactattGCGACGCGCACTCTGAGTAAACACTTCAATGATGATCGAGACGCCAAACGCGGCATACA‑GGTAACCTTACGGT‑ATGAGGCAA‑CCGGAACCTTCTGCCACCAGACTCAGACCAATCATAAACAGGAAACTAAGACA‑GAGCAGCA‑CCACGGCGGG‑TTGTTGATTACTGACGA‑GCACCAGCGGTTTGGATGCCAGCAACATAACCGCAACCGCAAACAAAAccgcgccca                                                                                                                                         >  2:125729/11‑234 (MQ=255)                                                                                               gCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GGGCTGGTTAACGAATC‑GCGTCAACGGTTTGGATGCACGCAAAATAACCGCCATCGCAATCACAACCGCCCCca                                                                                                                                         >  2:533983/1‑239 (MQ=255)                                                                                                                  gtaaTTTGGTTAACCACTTCGATGATGATGGAGAAGCCAACCGGGGCATACA‑GGTAACCTTTGGGA‑ATGTGGAAC‑CCGAAACCTCTGGCCACCAGACCAAGACCAATCATTAACAGGAAGCCCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGt                                                                                                                       <  2:933631/236‑1 (MQ=255)                                                                                                                           ttAAACACTTCGATTATGCGCGGAAAGCCAATCGCGGCATACA‑GGTACCCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGCCTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTGGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATccc                                                                                                             <  1:1085250/239‑1 (MQ=255)                                                                                                                             aaccccTTCGATGATGAACGAAAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGGGGAAA‑CCGAACCCTTCGGCCACCAGACTCAGACCAATCATTAACAGGAAGCCCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  1:915017/234‑1 (MQ=255)                                                                                                                             aaaccttgcggggattgaggaaaaGCCACCCGCGGCATACA‑GGAAACCTTTCGGA‑ATGGGGAAA‑CCGAAACCTTCGGCCACCAGACTCAGGCCAATCATTAACAGAAGGCACAGACA‑GAGCACCA‑CCACCGGGGG‑GTGCTGGTAAACAAATC‑GCGTCAGCGGTTTGGATGCCAGCAACTTAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  2:819460/217‑1 (MQ=255)                                                                                                                             aaaacctatggttatgcacGAAAAGCCAATC‑CGGCAAAAAGGGTACCCTTCAGGA‑ATGGGAAAC‑CCGAACCCTTCCACCCCCAACCCCAGACCAACAATTAAAAGGAACCCAAGCAA‑GAGCCCCA‑CCCCCGGGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGTTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  2:760089/221‑1 (MQ=255)                                                                                                                             aaaCACTTGGATGATTAACGAGAACCCAATCGGGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCCGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGGGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  1:867782/239‑1 (MQ=255)                                                                                                                              aaCACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCCCTACCGCCGCCATCATCACCGGCAGATGGTTAACCATcccaa                                                                                                           >  1:527950/1‑236 (MQ=255)                                                                                                                                    tCGCTGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCCGt                                                                                                    >  1:967841/1‑239 (MQ=255)                                                                                                                                    tCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGt                                                                                                    >  1:1006214/1‑239 (MQ=255)                                                                                                                                           ggataggaaaGCAAATCGCGGAATACA‑GGTAACCTTCCGGA‑ATGTGGAAC‑CCGAAACCTTCTCCCCCCAGACTCAGACCAATCATTACCAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGAATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAc                                                                                              <  1:1042752/230‑1 (MQ=255)                                                                                                                                                  aaggacccgccggcaaaaaa‑GGTAACCTTCGGAA‑ATGGGGAAA‑CCGAAACCTTCCGCCCCCAAACCCAGCCCAATCATTAACGGAAAGCTAGGACA‑GAGCACCA‑CCCCCGGGGG‑GTGCTGGTTAACGAAGC‑GCGTCAGGGGTTTGGATGCCAGCAACAAAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATcc                                                                                       <  2:834496/219‑1 (MQ=255)                                                                                                                                                        aaTCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACCACCGCCGCCACCATCCCCGGCAGAGGGTTAACCACCCCAACCCCAGAAAATACCCACTCCAAA‑‑gaga                                                                              >  2:995618/1‑239 (MQ=255)                                                                                                                                                          tCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCACATGGTTAACCATCCCTACTGCAGTAATTACCCCACCCAAC‑‑Gaaaaa                                                                            >  2:154342/1‑238 (MQ=255)                                                                                                                                                            ccggctcctcatgTAACTTTCAGGA‑ATGTGGAAC‑CACAACCCCTCCGCCCCCGGCACCATCCCAACAATAAACAGGACGCCCAGACA‑TGGACCCT‑CCTCCGTGGGTGTG‑GGGTTAACGACTC‑GCGTGAGCGGTTTGGATGCCAGCAACTTAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑Gagaaga                                                                           <  2:1052259/227‑1 (MQ=255)                                                                                                                                                                         ttcccTTTCGGA‑ATTGGGAAA‑CCGAACCCCTCCCCCACACACCTCAGACAAACCTATAACGGGACTCCCAGACA‑GACCACCC‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGTTTTGGATCCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGAt                                                              <  2:928256/236‑1 (MQ=255)                                                                                                                                                                         tAACCTTCAGGA‑ATGTGGAAA‑CCGAACCCTTCTGCCCCCCGCCTCAGACCAATCATTAACAGGAAGCCCAGCAG‑GAGCACCA‑CCCCCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAgg                                                                <  1:533983/237‑1 (MQ=255)                                                                                                                                                                          taCCCTTCCGG‑ATGTGGAAACCCGAAACCTCCTGCCACCAGACCAAGCCCAACCATAACCAGGAACCCAAGAC‑‑GAGCCCCACCCGCCGTGGG‑GTGCGGTTTAACGAATC‑GGGCCGCGGGTTGGGATGCCACCAACATACCCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGAt                                                              <  2:809005/238‑1 (MQ=255)                                                                                                                                                                           aCCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCCTCATCACCGGCAGATGGTTAACCATCCCTCCTGCAGTAATTACCGCATCCAAC‑‑GCGAAGCCGGctcacaggatg                                                             >  1:576117/1‑228 (MQ=255)                                                                                                                                                                             cTTTCGGAGATGTGGAAA‑CGAAACCCGTGTCCCACCGCACCGAGCCCAATAATGAACAGGAGACGCGGAAA‑GGGCCCCC‑CCCCGGGGGG‑GTGCGGGTTAACGAATC‑GGGTCAGCGGTTGGAATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGAc                                                           <  2:501251/239‑1 (MQ=255)                                                                                                                                                                                tCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGCTGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAAACGACGTCAAGGAAGACaa                                                         >  1:539455/1‑237 (MQ=255)                                                                                                                                                                                tCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGCAGGCGTCAAGGAGGACaacc                                                       >  2:691727/1‑237 (MQ=255)                                                                                                                                                                                tCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑ACAAAGAAGGCGTCAACGATGCCaa                                                         >  1:330698/1‑237 (MQ=255)                                                                                                                                                                                tCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCCACGTCAGAAATTACCCCCTCCAAC‑‑GAAAAAACGGCgcaaagaaagaaaaaa                                                       >  2:887703/1‑224 (MQ=255)                                                                                                                                                                                              acccaacccctccgccccccaGACGCAAACCAAACACAAACAGGAGGCCCAGACA‑GGCCACCC‑CCCCCGTGGG‑GGGCGGGTTAACTAACC‑CCGTCAGGTGTTGGAATTCCAGCAATATACCCCCCATACCATTCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACGGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTgacgac                                            <  2:539455/220‑1 (MQ=255)                                                                                                                                                                                                ccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGGTAACCATCCCTACTGCAGCAATTACCGCATCCAAC‑‑GAGACGACGGCGTCAAGGATGCCAATCttgttgacgccac                                          >  1:111425/1‑226 (MQ=255)                                                                                                                                                                                                    aaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGCAGGCGTCAAGGTTGACAATCTGTGTGCCGACCACCCaa                                     >  1:378853/1‑238 (MQ=255)                                                                                                                                                                                                           tGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTg                               >  1:212925/1‑238 (MQ=255)                                                                                                                                                                                                            gccccaggACTCAGCCCAATCATTAACAGGAAGCTCA‑ACACGGCCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAACC‑GCGTCAGCGTTTTGGATGCCAGCAAAATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGc                             <  1:691727/232‑1 (MQ=255)                                                                                                                                                                                                                         gACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACaa                                                         >  1:885940/1‑198 (MQ=255)                                                                                                                                                                                                                             cacaagtAACGGGAATCCCAGACG‑GGCCACCA‑CCCCTGTGGG‑G‑GGTGGGTAAAGAAACGGCGTAGGCGTGTTGAAGCGCACCAAAATACCCGCCATCGCAATCACTCCCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGc            <  2:967841/233‑1 (MQ=255)                                                                                                                                                                                                                             aaccATTAACAGGAAGCCAAGACA‑GGCCACCA‑CCCCGGTGGG‑GTGCTGGTTACAGAACC‑GCGTCAGCGGTTTGGATGCCAGCAACATACCCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGc            <  1:180152/236‑1 (MQ=255)                                                                                                                                                                                                                              aTCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCACC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCCCCCAGAAACCGGCGTACCCTTTACCTTGGcg           >  1:1054442/1‑238 (MQ=255)                                                                                                                                                                                                                                      cAGGCAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTACTCACCGCATCCACC‑‑GAGAAGACGGCGTCAAGGATTACAATATTAGGGACGACCACCCCGAAACCGGCGTAACCTTTTACGAGGCCgggaacaa   >  2:188651/1‑233 (MQ=255)                                                                                                                                                                                                                                         gAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGCCGTAGCCTTTACCGTGGCCGGAAACATg  >  1:995959/1‑239 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              CGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACA‑GGTAACCTTTCGGA‑ATGTGGAAA‑CCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACA‑GAGCACCA‑CCACCGTGGG‑GTGCTGGTTAACGAATC‑GCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATG  >  NC_000913/1900781‑1901246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑