Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	216,077	G→C	5.6%	intergenic (+98/+102)	nlpE → / ← yaeF	lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,077	0	G	C	5.6%	274.0 / 2.9	96	intergenic (+98/+102)	nlpE/yaeF	lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (41/47);  new base C (2/5);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGC  >  NC_000913/215839‑216312                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  gAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTAcg                                                                                                                                                                                                                                                   <  1:443259/239‑1 (MQ=255) gAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGGTTTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTAcg                                                                                                                                                                                                                                                   <  1:1072947/239‑1 (MQ=255)    aaaCCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCCGAA‑GGAGATATTTCAACAT‑TTTGAATTTGCACGTTTTTTTTAGGC‑CGGAAAAGGCGTTTAcgccg                                                                                                                                                                                                                                                >  1:486184/1‑239 (MQ=255)      cccGGTTTTTCTTTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTTCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCa                                                                                                                                                                                                                                              <  2:5058/238‑1 (MQ=255)            gtTGCTGGCAGTAGAAGGCCATTTTACGCTTGAGGGTAATCCGGATTCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCg‑g                                                                                                                                                                                                                                        <  1:290771/238‑1 (MQ=255)                     aGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAAATTTTACCCC‑ACCACGATTTCAGAAGTTTGGGGCCGACACCCGTCTTT‑AGAAACAAACAAACGCAAAAAAGGGCACAA‑GAGTATTTTTCAACAT‑TTTGAAATTTCACGTTTTTTGTATGC‑CGGGTAAGGCGTTTACCCCCCATcccgacatggggg                                                                                                                                                                                                                                >  2:651695/1‑227 (MQ=255)                            cgtccatttACGGTTGAGGGTAACCCGGATACGGGTGCGGCGACTAAAGTATTGGCCCCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGcc                                                                                                                                                                                                                       <  2:31580/233‑1 (MQ=255)                             gTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCCGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCCGAA‑GGATCTATTTCAACAT‑TTTGAATTTGCACGGTTTTTTTAGGC‑CGGGTAAGGCGGTTACGCCCCATCCG‑GCAATTGGGCTCAAcc                                                                                                                                                                                                                         >  1:87676/1‑235 (MQ=255)                              ttaCTTTACGCTTGAGGGTAACCCGGATACCGGTGCGCCGACTAAAGTATTGGCCCCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGcc                                                                                                                                                                                                                       <  1:978030/235‑1 (MQ=255)                                 cTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCCGGATTGCAGTAGTTTGGGGCAGGAACCCGTCTTG‑GGAACGAAACAAACGCCAAACAGGCCAGAA‑GGATATATTTTAACAT‑TTTGAATTTGCACGTTTTTTTTAGGC‑CGGATAAGGCGTTTATGCCGCATCCG‑GGaagtgggtgccaaacccgatg                                                                                                                                                                                                                  >  2:997053/1‑220 (MQ=255)                                 cTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGCAGTTTGGGGCAGCAACACGACTTG‑TGACAGCAACAAACGCAAAAAACGCCGACA‑GGATAAATTTTAACAA‑TTAGTATTTTCTCGGTTTTTTTAGTC‑CCGGTAAAGCGTTTACACGCCATCCG‑CCAATAGGGTTCAAACCCTgtg                                                                                                                                                                                                                   >  2:207217/1‑236 (MQ=255)                                           gAGGGTACTCCGGTTACCGGTGCCCCGATCAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCACCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGgc                                                                                                                                                                                                       <  2:533569/239‑1 (MQ=255)                                           gAGGGTAATCCGGATTCCGGTGCGACGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTGCCCCAACCAGGATTGCAGTAGGTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGgc                                                                                                                                                                                                       <  1:442567/239‑1 (MQ=255)                                            aGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAAATTTACCCCAAACAGGGTTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAAAAACACACCAAAAACAGGCCAGAA‑GGGTATATTTCAACAT‑TTTGAATTTGCACGATTTTTTTTGGC‑CGGACAAGGGGTTTACGCCCCACCCG‑GCAAGGGGTTCCAACCCCCGAT‑GCGACCCtcggg                                                                                                                                                                                                      >  2:1037550/1‑235 (MQ=255)                                                  aTCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGa                                                                                                                                                                                                                    <  1:974146/220‑1 (MQ=255)                                                      ggATCCCGGTGCCCCGACTAAAGTTTTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCa                                                                                                                                                                                            <  2:1086536/239‑1 (MQ=255)                                                       gttACCGGTGCCCCGAATAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCAt                                                                                                                                                                                           <  2:916828/237‑1 (MQ=255)                                                                 gcccGCCTAAGGTTTTGTCCCCCGATTCGGCATGG‑AAATTTTACCCCAACCGGGATTGCAGTAGTTTGGGGCATTAACCCGTTTTG‑AGACCGAAACAAACGCAAACCAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  2:818762/237‑1 (MQ=38)                                                                 cgcCGACTAAAGGATTGGCACCCGGTACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Ac                                                                                                                                                                                <  1:41400/238‑1 (MQ=255)                                                                  gcCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  1:372523/238‑1 (MQ=255)                                                                  cccaaCTAAAGTTTTGGCCCCCGGTACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  2:87676/234‑1 (MQ=255)                                                                    cGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAAAGCCTGAT‑GCGACGCTGGCGCGCCTTATCATGCCT‑ACA‑Acccc                                                                                                                                                                             >  1:1016878/1‑238 (MQ=255)                                                                       cTAAAGGTTGGGCACCCGATTCGGCAGGG‑AAATTTTCCCCCAACCAGGATTGCCGTAGTTGGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACCCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTc                                                                                                                                                                         <  2:946254/239‑1 (MQ=39)                                                                        tAAAGTTTTGGCACCCGATACGGCAGGG‑AAATTTTCCCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑GGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        <  2:7698/239‑1 (MQ=255)                                                                        tAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        <  1:644113/239‑1 (MQ=255)                                                                        tAAAGGATTGGCACCCGGTTCGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTc                                                                                                                                                                         <  1:137742/238‑1 (MQ=255)                                                                           aGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTTTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGGGCTCAACGCCCGAT‑GCGACGGTGGGGGGGCTTCTAATGCCC‑AAACAcccccccaca                                                                                                                                                                       >  2:921116/1‑234 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACa‑aac                                                                                                                                                                               >  1:878311/1‑222 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑Acc                                                                                                                                                                               <  2:878311/224‑1 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAAACCCTGAT‑GCGACGCTGGCGCGGCTTATCATGCCT‑CCC‑ACCCCCCCCAAACCTTaa                                                                                                                                                                >  1:586214/1‑239 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAAGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCCGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTaa                                                                                                                                                                >  1:163646/1‑239 (MQ=255)                                                                                tGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGGTTGGGGCAGGAAACCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCCGAA‑GGGTATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAAGCGGTTTCGCCCCAACCG‑GGAAAAGGGCTCAAACCCCGATGGGGGCGC‑GGCGCGGCTTTTCCTTCCT‑CCA‑Aaccccccccacccctaa                                                                                                                                                                >  2:685496/1‑228 (MQ=255)                                                                                    aCCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGGGCTCAACCCCCGAT‑GCGGCGCGGGGCCGGCTTTTCATGCCTCAAA‑CCCCCCCCCACACCTTAGGGc                                                                                                                                                             >  2:828043/1‑239 (MQ=255)                                                                                       cGATGCGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTTGTTTGGGGCATTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:1020974/239‑1 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAATAGTTTGGGGCAGTAAACCGTCTTG‑AAACAGAAACAAACGACAAACAGGCCAGGA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGCTAAAGCGTTTACGCCGCCACCG‑GCAATGGGGGCCAACCCCCGAT‑GCGGCGCTGGCGCGGCTTACACTACCT‑TCA‑ACCCCCCCCCTACCTTAcggcggga                                                                                                                                                         >  2:221360/1‑237 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTTACCCCAAACAGGATTGCATTAGTTTGGGGCAGTAACCCGTCTGG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑TCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:129787/239‑1 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTTACCCCAAACAGGATTGCAGTAGTTAGGGGCAGTAACCCGGCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:499533/239‑1 (MQ=255)                                                                                       cGATACGGCAGGG‑AAATTTGACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGAt                                                                                                                                                         <  1:48609/239‑1 (MQ=255)                                                                                        gATACGGCAGGG‑AAATTTTTCCCCAACCAGGATTGCAGTAGGTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCATCAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATa                                                                                                                                                        <  1:651695/239‑1 (MQ=255)                                                                                                aGGG‑AAATTTTGCCCCAACCAGGGTTGCAGTAATTTGGGGCAGAAACCCATCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCAtt                                                                                                                                                 <  1:579225/238‑1 (MQ=255)                                                                                                  gg‑AAATTTTACCCCAACCAGGATTGCAGTAGGTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGGGCTCAACGCCTGAT‑GCGACGCTGGGGGGGCTTATCATGCCT‑ACA‑AACCCCCCCCAACCTTAAGGCGGGAAAGGAAttt                                                                                                                                                >  1:765187/1‑237 (MQ=38)                                                                                                      aaTTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACttt                                                                                                                                           <  1:997053/239‑1 (MQ=255)                                                                                                        ttttACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATc                                                                                                                                                                                             <  1:686763/189‑1 (MQ=255)                                                                                                           tACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                                                                                                                      <  1:221360/239‑1 (MQ=255)                                                                                                           tACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                                                                                                                      <  1:828043/239‑1 (MQ=255)                                                                                                               caaaCCA‑GATTTCGGTAGTTTGGGGCAGTAACCCGGTTTGAAGAC‑GAAACAAACGCAAAAAATGCCAGAA‑TGATATAGTTCAACCT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTAGCAC‑CGGGt                                                                                                                                <  2:842014/237‑1 (MQ=37)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTTGCCCGTCTTCTCATGCCT‑CCa‑a                                                                                                                                                                                 >  2:686763/1‑189 (MQ=255)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCa                                                                                                                              >  1:391617/1‑239 (MQ=255)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCa                                                                                                                              <  1:764869/239‑1 (MQ=255)                                                                                                                  aCCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCCCATACCTTAAGGCGGATAAGGCATTTACTTTATCCA‑CGGGCCa                                                                                                                              >  2:707594/1‑239 (MQ=255)                                                                                                                            cAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATa                                                                                                                                                        <  1:285638/204‑1 (MQ=255)                                                                                                                            cAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACaaa                                                                                                                   >  1:762997/1‑239 (MQ=255)                                                                                                                                 gtTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGa                                                                                                               >  1:212732/1‑238 (MQ=255)                                                                                                                                    tGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGTTCAACGCCTGAT‑GCGACGCTGGCGCGCCTTATAATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAAGCATTTACTTTATACC‑CGGGCCAAACA‑AAAAAAACCAccc                                                                                                            >  2:997746/1‑238 (MQ=38)                                                                                                                                    tGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGTT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCCCATACCTTAAGGCGGCGAAGCCATTTACTTTA‑CACACGGGCCAAACCAAACAAAGAGAca                                                                                                             >  2:317752/1‑237 (MQ=38)                                                                                                                                      gggCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGGCTTATCATGCCT‑CCA‑ACCCCCCTCATACCTTAAGGCGGCTAAGGAAttttc                                                                                                                                              >  2:285638/1‑202 (MQ=255)                                                                                                                                       ggCCGTAACCCGTCTTG‑AGACAGAAAAAAAAGAAAAACAGGACAAAA‑GGTTATA‑TTCAAAATGTTTGAATGTGCACGTTTTTGGTAGGC‑CGGAAAAGGCGTTTACGCGGAATCGG‑GCAATGGTGCTCAACGCCGGAT‑GCGAGGCTGGCGCGTCTTATCAGGCAA‑ACA‑CCCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACA‑AACAAAGCGACCCGcc                                                                                                         <  2:953608/238‑1 (MQ=255)                                                                                                                                           gTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAg                                                                                                                                                               <  2:640379/182‑1 (MQ=255)                                                                                                                                           gTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAg                                                                                                                                                               >  1:640379/1‑182 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGTA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGTT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCCTACCTTAAGGCGGGTAAGGCATTTTATTTTTCAC‑CGGGCCAAACC‑AACAAAACAACCCCCCTTGata                                                                                                   >  2:899855/1‑239 (MQ=38)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑CCA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AAAAAAGCGACCCCCCTTGaaa                                                                                                   >  2:1061242/1‑237 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        <  2:877118/172‑1 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCa                                                                                                                                                                        >  1:877118/1‑172 (MQ=255)                                                                                                                                            tAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGata                                                                                                   >  1:719344/1‑239 (MQ=255)                                                                                                                                                            aaaaaacaaacCAAAAACAGGCCAAAA‑GGATAATTTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTc                                                                                    <  1:921116/236‑1 (MQ=255)                                                                                                                                                                aacaaacCTAAAGCAGG‑CAGACGGGATTTATTTAAACGT‑TTTGAATTTTCCGGTTTGTTTGAGGC‑GGGCTAGGTGTTTTACGCCCCATCCG‑GAAAGTGTGATCAACGACTTGT‑GCGACGCTGGCGCTTTTTACCATGCCT‑ACA‑ACCCCCTTCATCCCTTAAGGCGGATAGTGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGt                                                                                <  2:615262/239‑1 (MQ=255)                                                                                                                                                                   aaccccAAAACGGGCCAGAA‑TTTTATTTTTTAAATT‑TTTGAATTGGCACGTTTTTTGTAGGC‑CGGATAGGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAG‑GCGACGCTGGCGCGTTTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTACCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGcc                                                                             <  2:391617/234‑1 (MQ=255)                                                                                                                                                                      cacAAAACAGTCTGGAA‑GGATTTTTTTGAACAT‑TTTTAATTGGCATGTTTGTTGTGGGC‑CGGATAGGGCGTTTCCGCCGCTGCCG‑ACAATGGTGCTCACCGCCGGAT‑GCGACGGGGGCGCGTCTTATCTTGCCT‑ACA‑CCCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCaa                                                                          <  2:585703/237‑1 (MQ=255)                                                                                                                                                                        cAAAACAGGCCAGAA‑GGATTAATTTCACCTT‑TTTGAATTGTCACGTTTTTTGAAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTTTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                                                        <  1:899855/239‑1 (MQ=39)                                                                                                                                                                                 ccAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑ACCAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGTCCCCAACATAcgtaa                                                                 >  1:283182/1‑233 (MQ=255)                                                                                                                                                                                     aa‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTCAGGCGGGTAAGGCATTTACTTTATCAC‑CGGGCCAAACC‑ACCAAAGCGACCCGCCTTGTTATAAATCCCAGGCTTCAGGTGCCCAACAAAcggaaaatccg                                                           >  2:702945/1‑229 (MQ=38)                                                                                                                                                                                          ttatatTTCAACAT‑TGTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑CCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGttt                                                        <  1:707594/237‑1 (MQ=255)                                                                                                                                                                                          atatatTTCAACAT‑TTTGAATTTGCACGTTGTTTGGAGGC‑CGGATAAGGCGTTTCCGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGttt                                                        <  1:702945/238‑1 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGtt                                                         >  1:773532/1‑236 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCCCCTTGATATAAATCCCAGGCTTCAGGTGCCCAAAATACTGAAACCGGGttttg                                                      >  2:556681/1‑237 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACCCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGt                                                                                                                                >  1:55474/1‑166 (MQ=255)                                                                                                                                                                                           tatatTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACCCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGt                                                                                                                                <  2:55474/166‑1 (MQ=255)                                                                                                                                                                                                ttCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCACCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCGTGATATAAAACCCAGCATTCAGGGGCCCAACAAACTGGAACTGCGTTTCTGGCtt                                                 >  2:274666/1‑239 (MQ=38)                                                                                                                                                                                                 ccaccAT‑TTTGAATTTGCCCGTTTTTGTGAGGC‑CGGAAAGGGCGTTTCCGCCCCATCCG‑GAAATGGGCCACAACCCCTGAT‑GCGACGCTGGCGCGTCTTACCATGCCT‑ACA‑ACCCCCCTAATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTa                                                <  1:1061242/235‑1 (MQ=255)                                                                                                                                                                                                   aaCAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGGGCCCAACATACTGTAACTGCGTTTCTGGCttca                                               >  1:475205/1‑236 (MQ=255)                                                                                                                                                                                                     cAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAAACAACTGTAAATTCGTTTCTGGCTTTAAc                                             >  1:354368/1‑238 (MQ=255)                                                                                                                                                                                                         ttttgtttttgggtttttttGGAGGCGCTGATAGGGCGTTTACCCCGCTCCCG‑GAAATGGTGCCCAACGCCTGAG‑GCGACGCTGCGTCGTCTTATCAGGCCT‑ACA‑ACCCCCCTCAGACCGTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCa                                          <  2:163646/225‑1 (MQ=255)                                                                                                                                                                                                         tttGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATCTAAATCCCAGGCTTCAGGGCCCCAACAAACTTGAACTCGGTTTTTGGCTTAAACGCaa                                         >  2:610082/1‑238 (MQ=38)                                                                                                                                                                                                          tgtaaTTTGCCTGTTTTTTGTAGGC‑CGAATAAGGGGTTTCCGCCGCATCCG‑GCAAGGGGCCTCAACGCCTGAT‑GCGACGCTGGGGGGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCaga                                        <  2:719344/236‑1 (MQ=255)                                                                                                                                                                                                                  gCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCAc                                >  1:880917/1‑239 (MQ=255)                                                                                                                                                                                                                     cGTTTTTTGTAGGC‑CGGATAAGGCGGTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCaa                             <  1:685496/239‑1 (MQ=255)                                                                                                                                                                                                                       ttttttGGTGGC‑CGGAATAGGGGTTTCCCCCGTATCCC‑GAAATGGTCCTCAACCCCTGAT‑GCGACGCGTGCGGGTCTTATCATGCCT‑ACA‑ACCCCCCCCATACCTTAAGGCGGAAAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATa                           <  2:475205/239‑1 (MQ=255)                                                                                                                                                                                                                       ttttttGGTGGC‑CGGA‑AAGGGGTTTTCCCCCCCACCGCGCAATGGGGCCCACCCCCTGAT‑GGGGCTCTGGCGCGTATTATCATGCCT‑ACA‑CCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATa                           <  2:762997/239‑1 (MQ=255)                                                                                                                                                                                                                                ggC‑CGGAAAGGGTGTTTCCGCCCTACCCG‑GCAATGGTGCCCAACCCCTGAT‑GCGCCGCTGGCCGGTCTTATCATCCCT‑ACA‑ACCCCCCTCATCCCTTAAGGGGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGc                  <  2:212732/239‑1 (MQ=255)                                                                                                                                                                                                                                 gC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACCTACTGTACCTGCGTTTCTGGCTTAAACGCAGAGCACTACCCAATACGCaaatcga                 >  1:510492/1‑236 (MQ=39)                                                                                                                                                                                                                                     ggATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATAAAAATcc                                                                                            >  1:712870/1‑161 (MQ=255)                                                                                                                                                                                                                                     ggATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATAAAAATcc                                                                                            <  2:712870/161‑1 (MQ=255)                                                                                                                                                                                                                                       aTAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACCGTAACTGCGTTTCTGGCTTAAACGCAGAGCCATCACCAATACGCATATGCATCCGa            >  1:849471/1‑239 (MQ=255)                                                                                                                                                                                                                                       aTAAGGCGTTTACGCCGCATCCG‑CAAAGGGTGCCAAACGCCGTAG‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCCC‑CGGGTCAAACC‑AACAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAg             <  1:610082/238‑1 (MQ=255)                                                                                                                                                                                                                                                ttACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATCCCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATcc                                                                                            <  1:594820/150‑1 (MQ=255)                                                                                                                                                                                                                                                ttACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATcc                                                                                            >  2:594820/1‑150 (MQ=255)                                                                                                                                                                                                                                                ttACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGGCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAAAGACATCACCAAAAAGCAAATACATAAGAACGGCAggg   >  2:454203/1‑239 (MQ=255)                                                                                                                                                                                                                                                 tACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGCCCCGCCTTGATATAAATCCCAGGCTTCAGGGGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATCAGCATATGCATAAGATCGGAAGGGc  >  2:8102/1‑239 (MQ=255)                                                                                                                                                                                                                                                 tACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAACCGCAAAACCATCACCAATAAGCAAAAGCATCAAATCGGCCgggg  >  2:745783/1‑238 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  GAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGG‑AAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTG‑AGACAGAAACAAACGCAAAACAGGCCAGAA‑GGATATATTTCAACAT‑TTTGAATTTGCACGTTTTTTGTAGGC‑CGGATAAGGCGTTTACGCCGCATCCG‑GCAATGGTGCTCAACGCCTGAT‑GCGACGCTGGCGCGTCTTATCATGCCT‑ACA‑ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAC‑CGGGTCAAACC‑AACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGC  >  NC_000913/215839‑216312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑