Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,171,546	G→A	12.9%	pseudogene (182/299 nt)	gatR ←	pseudogene, repressor for gat operon, interrupted by IS3, split galactitol utilization operon repressor, fragment 2, split galactitol utilization operon repressor, interrupted

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,171,546	0	G	A	12.9%	218.4 / 2.0	85	pseudogene (182/299 nt)	gatR	pseudogene, repressor for gat operon, interrupted by IS3, split galactitol utilization operon repressor, fragment 2, split galactitol utilization operon repressor, interrupted
Reads supporting (aligned to +/- strand):  ref base G (52/19);  new base A (5/7);  total (58/27)
Fisher's exact test for biased strand distribution p-value = 4.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGG  >  NC_000913/2171311‑2171780                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              tatCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTTGAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGG‑GGGTAGGGTCAATCATCCCTTCGTGGATCATTTTTACTGCCGCCTGCGCGATTTTGTTTTTTTGCGGGCT‑GGCGGGC‑TGAAAT‑CGCCCTTTAAA‑ACCGCCTTCCTGGGGCTC‑GGTTTTCATTTTACCAAAC‑a                                                                                                                                                                                                                                           >  2:354939/1‑239 (MQ=18) tatCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTTACTGCCGCCTGCGCTATTCTGTTTTTTTGCGCGCT‑GGCGGGC‑TTAAAT‑CGCCCTTTAAA‑ACCGACTTCCTGGGGCTC‑GGGTTCACCATTACCAAAC‑a                                                                                                                                                                                                                                           >  1:557800/1‑239 (MQ=21)   tCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGCCTTCCTGGGTCTC‑GGTTTCCATATTCCCAGAC‑ctt                                                                                                                                                                                                                                         >  1:754247/1‑239 (MQ=255)      aaTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGGTCATTTTTACTTCCGCCTGCGCCATTCTGTTTTTTTGCCCGCT‑GGCGAGC‑TGAAAT‑CGCCCTTTAAA‑CCCCACTTCCTGGGGCCC‑GGTTTCCCTATTAACAAAC‑aata‑tt                                                                                                                                                                                                                                     >  2:747162/1‑239 (MQ=21)      aaTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGG‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGCGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTTACCATTACCAGAC‑attt‑tt                                                                                                                                                                                                                                     >  1:1045416/1‑239 (MQ=255)        tgtgATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCCTGGATCATTTTTACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCC‑GGCGGGC‑TGAAAT‑CGCTCTTTAAA‑CCCCACTTCCTGGGTCTC‑GGTTTCACCATTACCAGAC‑ATAA‑tttt                                                                                                                                                                                                                                   >  2:879832/1‑239 (MQ=255)        tgtgATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAAAACCTCGCTTAGGCCTGTGTCCCTATTACGT‑GGGTAGGAACAGTCATCCCTTCGTGGGTCATTTTTACTGCCGCCCGCGCTTTTTTGTTTTTTTTCGCGCG‑TGCGGGC‑GGAAATCCCCTCTTTAAA‑‑CCGGCTTCCTTGGTCCC‑GGGTTTACCTTTTCCCAAC‑AATA‑tttt                                                                                                                                                                                                                                   >  2:671929/1‑239 (MQ=17)        tgtgATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGACTTAGACCGGAACAATTTGAAAACAAGAACCCTCCTTAAGCCTGTGTCCCAAATAACT‑GGGTAGGGTCAGCGATCCCTTCGTGGGTCCTTTTGAATGCCACACGGGCTATTTTGTTTTTTGGCCGGCC‑GG‑GCGCGTGCACA‑TGCGCCTTGAA‑CCCGACTTCCCGGGGTCC‑GGTTTCCCTATTTCAAAAC‑ATTA‑attt                                                                                                                                                                                                                                   >  2:969784/1‑239 (MQ=9)                cAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAACAACCTCGCTTAGGGCTGTGTCCATATTACGG‑GGGGAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTTTTTTTTTTTGCGCGCT‑GTGGGGC‑TGCAAT‑AGCTCTTTTAA‑ACCGCCTTTCTGGGGTCC‑GGGTTTACCATTACCAGAA‑AATA‑TTTTTGCCTCa                                                                                                                                                                                                                            >  2:505195/1‑238 (MQ=18)                cAAGCGGTGGCGGCGGCACAGTTGGTGTGGGGGCCTAAGTCCGGAACAATTTGAAACCAAGACCCTCGGTTGGGCTTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCAc                                                                                                                                                                                                                           <  1:844108/239‑1 (MQ=38)                                 acaGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGGTCAGTCATCCCTTCGTGGATCATTTTGACTGCCCCCTGCGCTCTTCTGTTTTTTTGCGCGCT‑GGCGAGC‑TGAAAT‑CGCCCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCCCTATTACCACAC‑CTTATTTTTTGCCCCCCCCCCATGAAAGcgcga                                                                                                                                                                                                           >  2:214399/1‑238 (MQ=25)                                             gcggcCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGGTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCCCCGCCATGAAAGCGCGTAA‑CCACGCCtttt                                                                                                                                                                                             >  1:465890/1‑239 (MQ=255)                                                      gTCCGGAACATTTTGAAAACGAGATCCTCGTTTGGGCTGGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTGGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCgaga                                                                                                                                                                                    <  1:100663/239‑1 (MQ=255)                                                        ccGGAAAAATTTGAAAACAAGAACCTCGTTTAGGCCTGTGTCCATATTACGG‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAC‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCgaga                                                                                                                                                                                    <  1:671929/237‑1 (MQ=255)                                                           gAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAgcg                                                                                                                                                                               >  1:178562/1‑239 (MQ=255)                                                            aaCAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTGCGG‑GGGTAGGATCATTCACCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAgcg                                                                                                                                                                               <  1:730496/238‑1 (MQ=255)                                                                 tttGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTaa                                                                                                                                                                                                         >  1:620902/1‑208 (MQ=255)                                                                 tttGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCCCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTTTAGAAAACGCAAATc                                                                                                                                                                         >  2:238051/1‑239 (MQ=255)                                                                     aaaaaaAGACCCCCGGTTGGGTCT‑TGTCCCTTTTTCGTGGGGTGGGATCAGTCATCCCTTCGGGGTGTATTTTGCCTGCCGCCTGCGCTTTTCTGTTTTTTGGGGCGTG‑GGCGAGC‑TGAAAC‑CGCTCTTTAAA‑CCCCGCTTCCTGGGTCTC‑GGTTTCACTATTACCAGCC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCa                                                                                                                                                                     <  2:742286/239‑1 (MQ=255)                                                                      aaaCAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCAc                                                                                                                                                                    >  1:322893/1‑239 (MQ=255)                                                                            gAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCCCTATTACCAGAC‑ATTA‑TTTTAGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGCGAAAGCGCAAATCGGCACGGCTTg                                                                                                                                                              >  1:248427/1‑239 (MQ=255)                                                                                   gCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTTTTCGAGAAAGCGCAAATCGGCCCGGATTGTAGCTTc                                                                                                                                                       >  2:153788/1‑239 (MQ=255)                                                                                   gCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCtt                                                                                                                                                        >  1:57460/1‑238 (MQ=255)                                                                                    cTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTcc                                                                                                                                                      >  1:627846/1‑239 (MQ=255)                                                                                                   atatTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCCAATAc                                                                                                                                        >  1:350105/1‑238 (MQ=255)                                                                                                   atatTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATAc                                                                                                                                        >  1:789906/1‑238 (MQ=255)                                                                                                        aCGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAgcgc                                                                                                                                                                                                             >  1:646064/1‑165 (MQ=255)                                                                                                        aCGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGCGGCAGCAAATACTCCCg                                                                                                                                   >  1:281094/1‑238 (MQ=255)                                                                                                        aCGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCCTTTTGTTCGAGAAAGCGCAAATCGGAACGGATTGACCCTTCCAAGGCAGCAAATACTCCCGc                                                                                                                                  >  1:150097/1‑239 (MQ=255)                                                                                                         cGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCa                                                                                                                                                                     >  2:15596/1‑203 (MQ=255)                                                                                                         cGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCa                                                                                                                                                                     <  1:15596/203‑1 (MQ=255)                                                                                                         cGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGcc                                                                                                                                 >  1:780329/1‑239 (MQ=255)                                                                                                          gT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTCCCAGAC‑ATTA‑TTTTCTCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGccc                                                                                                                                >  1:221138/1‑238 (MQ=255)                                                                                                          gT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCCGCAAAAACCCCCGcaa                                                                                                                                >  2:827983/1‑237 (MQ=255)                                                                                                              ggTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGa                                                                                                                              >  1:812371/1‑238 (MQ=255)                                                                                                              ggTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGa                                                                                                                              >  1:384829/1‑238 (MQ=255)                                                                                                               gTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCCCGGATTTTCGCTTCCTAGGCAGAAAAAACCCCCCCCAAAt                                                                                                                             >  2:81320/1‑238 (MQ=255)                                                                                                               gTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATAATCCCACCAGag                                                                                                                             >  2:177795/1‑237 (MQ=255)                                                                                                               gTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCCCTTCCGAGGCAGCAAAAACTCCCCCCaaac                                                                                                                             >  2:357405/1‑235 (MQ=255)                                                                                                                  ggATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGGGGCAGCAAAAACTCCCCCCAAACCCTg                                                                                                                         >  2:1033342/1‑239 (MQ=255)                                                                                                                   gATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGa                                                                                                                        >  1:949536/1‑239 (MQ=255)                                                                                                                   gATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CAACGCCTTTTTGTTCTAGAAAGCGCAAATCGGCACGGATTTTCCCTTCCGAGGCAGCAAAAACCCCCGCCAGATCCGGa                                                                                                                        >  2:241004/1‑239 (MQ=255)                                                                                                                          ctcctcTTCGTGGTTCTTTTTCTCTCCCCCCTGCGTTTTTTTTTTTTTGGGCG‑GCTGGGGGAGC‑TGAAAT‑CGCTCTTTAAC‑CCGGACTTCCGGGGTCTC‑GTTTCCACTATTCCCAGAC‑TTTA‑TTTCCCCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCa                                                                                                                 <  2:1045416/234‑1 (MQ=255)                                                                                                                            gcccTTGGGGTATCATTTGGACTGCCGCCTGGGCTTTTCTGTTTTTTGGCGCGCT‑GGCGATC‑CAAAAT‑CGCTCTTTAAC‑CCCGCCTTCCTGGGTCCC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACg                                                                                                               <  1:750815/238‑1 (MQ=255)                                                                                                                            cccttgcgggtatattttgGACCGCCGCCTGCGCTTTTCTGTTTTTTGGCGCGCT‑GGCGAGG‑TGAAAT‑CCCTCTTTAAC‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACg                                                                                                               <  1:586664/224‑1 (MQ=255)                                                                                                                             cccTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAAATCCTCAACAAACACgg                                                                                                              >  2:814887/1‑239 (MQ=255)                                                                                                                              ctttCGTGGATCATTTTGACTGCCGCCTGCGTTTTCCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCCCTTTAAC‑CCCGACTTCCTGGGTCCC‑GGTTTCACTTTTCCCAGAC‑ATTA‑TTTTCCCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGt                                                                                                             <  1:1033342/237‑1 (MQ=255)                                                                                                                              ccTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTTTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGACGCAAAAACTCCCGCCaaaacccaaaaaagaaagg                                                                                                              >  2:518422/1‑220 (MQ=255)                                                                                                                                 tCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGCTTGTCCCTTCCGGGGCAGCAAATACTCCCGCCAGTCCCTGacaacaaacgggtc                                                                                                           >  2:651965/1‑225 (MQ=255)                                                                                                                                 tCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACCCCCGCCAAACCCTGAACAAGACCGGTTc                                                                                                           >  2:586664/1‑238 (MQ=255)                                                                                                                                  cGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTc                                                                                                           >  1:777111/1‑237 (MQ=255)                                                                                                                                   gTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAAAACTCCCCCCAGATCCCGAAAAAGAACGGTTcccc                                                                                                        >  2:109740/1‑238 (MQ=255)                                                                                                                                       aTCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCGGCAAATACTCCCGCCAGATCCTGAACAACCACGGTTCCCTGtta                                                                                                    >  1:1085816/1‑238 (MQ=255)                                                                                                                                       aTCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGttc                                                                                                    >  1:106057/1‑239 (MQ=255)                                                                                                                                       aTCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAACCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCCCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGACCGGTTCCCTGttc                                                                                                    >  1:511678/1‑239 (MQ=255)                                                                                                                                         cATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGttcat                                                                                                  >  1:263054/1‑239 (MQ=255)                                                                                                                                          tttttGCCTCCCCCCTGTTCTTTTTTTTTTTTTGGCGGTCT‑GGAGAGC‑TAAAAT‑CGC‑CTTTAAACCCCGCCTTCCGGGGTCTG‑GTTTCCCCTTTTCCCAAAC‑TTTA‑TTTCCGCCGCCCCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGttcatt                                                                                                 <  2:557800/238‑1 (MQ=255)                                                                                                                                                     cccctgctttttttttttttttGGCGCGCT‑GGCGAGC‑TAAACT‑CGCTTTTAAAC‑CCCGCCTTCCGGGGTTCC‑GGTTCCACTTTTCCCAAAC‑TTTA‑TTTCCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGga                                                                                      <  2:384829/223‑1 (MQ=255)                                                                                                                                                     ccccTGCCCTTTTCGGTTTTTTGGCGCGCT‑GGGAAGC‑TGAAAT‑CGCTTTTAAAA‑CCCGACTTCCTGGGTCTG‑GGTTTCATTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGga                                                                                      <  1:879832/237‑1 (MQ=255)                                                                                                                                                      ccctgggtttttgtGTTTTTTGGGCGGCTGGGCGATC‑GAAAAC‑CCCTTTTAACA‑CCCG‑CTTCCCTGGGCTCGGGTTTCATTTTTCCCGAAC‑TTTT‑TTTCCGCCGCCCCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGga                                                                                      <  2:106057/226‑1 (MQ=255)                                                                                                                                                      cccTGCGCTATTTTGTTTTTTGGCGCTCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCGGGGTCTC‑GTTTTCACTATTACCAGAC‑ATTA‑TTTCCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGgat                                                                                     <  1:238051/238‑1 (MQ=255)                                                                                                                                                                  cgtttttttGGCGGGCT‑GGCGAGC‑TAAAAT‑CGCTTTTTAAC‑CCGGATTTCCTGGTTCTG‑GGTTCCATTTTTACCAGAC‑ATTT‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCt                                                                         <  1:638134/236‑1 (MQ=255)                                                                                                                                                                     ttttttGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATa                                                                                                                                         >  1:116590/1‑172 (MQ=255)                                                                                                                                                                        tttGGCGCGCT‑GGCGAGC‑TGCAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCCCTCATTGGATGACCTTATTCCTTTGCTc                                                                   >  1:573647/1‑239 (MQ=255)                                                                                                                                                                          tGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATa                                                                                                                                         >  1:728105/1‑167 (MQ=255)                                                                                                                                                                          tGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCCGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCga                                                                 >  1:236241/1‑239 (MQ=255)                                                                                                                                                                                        aggtcgAAAT‑CGCTCTTTAAC‑CCCGCCTCCCGGGGTCCG‑GGTTTCCCTATTCCCAGAC‑ATTG‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAAGCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCCGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATc                                                      <  1:109740/233‑1 (MQ=255)                                                                                                                                                                                        aGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGAAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGt                                                    >  1:97227/1‑239 (MQ=255)                                                                                                                                                                                            tGAAAT‑CCCTTTTTAAA‑CCCCACTGCCGGGGTTTG‑GGTTTCCCTTTTCCCAAAC‑ATTT‑TTTTCGCCCCCCCGGCATGAAAGCGCGTAA‑CCCCGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGt                                                    <  2:780329/236‑1 (MQ=255)                                                                                                                                                                                               aaaatcccctttaaaaccgcgtctCCCGGGTTCC‑GTTTTCAATATTACCGGCC‑GTTT‑TTTTCGCCGCCCCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCAtt                                                <  1:153788/216‑1 (MQ=255)                                                                                                                                                                                                ct‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCGAAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGGCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTAc                                             <  1:745331/238‑1 (MQ=255)                                                                                                                                                                                                 accgttctTTAAA‑CCCGACTTCCGGGGTCGC‑GTTTTCCCTATTACCAGAC‑ATTA‑TTTTCGCCCCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTAc                                             <  1:814887/234‑1 (MQ=255)                                                                                                                                                                                                   cGCTTTTTAAC‑CCCGTCTCCCGGGGTCTC‑GTTTTCCCT‑TTTCCAGTCAATTT‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTAccc                                           <  1:518422/239‑1 (MQ=255)                                                                                                                                                                                                      tctTAAAA‑CCCGGTCTCCGGGGCCCC‑GTTTTCACTTTTCCAAGCA‑ATTT‑TTTCCCCCACACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCata                                        <  1:958015/239‑1 (MQ=255)                                                                                                                                                                                                          tAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTAATTCCTTCGCCCGAATGAATCAATCGTCATTTACCCAAATTCa                                    >  2:859284/1‑239 (MQ=255)                                                                                                                                                                                                                   aCTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATtttg                              >  1:839012/1‑237 (MQ=255)                                                                                                                                                                                                                               tc‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGa                >  1:622470/1‑239 (MQ=255)                                                                                                                                                                                                                                       cACTATTCCCAACC‑TTTA‑TTTCCCCCGCCCCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGa         <  1:272832/239‑1 (MQ=255)                                                                                                                                                                                                                                        aCTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCCCCCGAATGAATTCATCGCCCTTTACCCATATTCAATTTTGGCTAGGTTAAAAAAAGGGGGAc        >  2:309193/1‑239 (MQ=255)                                                                                                                                                                                                                                          ttttACCACAC‑TTTA‑TTTCCCCCGCTCCGCCATGAAAG‑GCGTAATCCACGCCTTTTTGGTCGAAAAAGCGCAAATCGGCACGGATTTGCGTTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCa      <  1:651965/237‑1 (MQ=255)                                                                                                                                                                                                                                          tATTCCCAAAC‑TTTT‑TTTGCCCCCCCCCGCCATGAAAGCGCGTAA‑CCCCGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCa      <  2:977558/239‑1 (MQ=255)                                                                                                                                                                                                                                            ttACCAGAC‑TTTA‑TTGCCGCCACACCGCCATGAAAGTGCGTAA‑CCCCGCCTTTTTGTTCGAGAAAGCGCAAACCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCAtt    <  1:519070/239‑1 (MQ=255)                                                                                                                                                                                                                                              aCCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCCCTAATTGGATAATCTTATTCCTTCGCTCGAATGAATTCATCGTCCTTTACCCATATTCACTTGTGGCTAGAGAAAACGAAaggggcacatggg  >  2:338424/1‑231 (MQ=255)                                                                                                                                                                                                                                              aCCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAAtt                                 >  1:290858/1‑208 (MQ=255)                                                                                                                                                                                                                                              aCCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTTTGGCTAGTGTAAACGAAGGGGGACCATTgg  >  1:977558/1‑239 (MQ=255)                                                                                                                                                                                                                                                  gAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCga                                                                 >  1:727971/1‑172 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              TATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGT‑GGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCT‑GGCGAGC‑TGAAAT‑CGCTCTTTAAA‑CCCGACTTCCTGGGTCTC‑GGTTTCACTATTACCAGAC‑ATTA‑TTTTCGCCGCACCGCCATGAAAGCGCGTAA‑CCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGG  >  NC_000913/2171311‑2171780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑