Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,091,192	T→A	18.2%	I392F (ATT→TTT)	frvB ←	putative PTS enzyme, IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,091,192	0	T	A	18.2%	188.4 / 8.8	86	I392F (ATT→TTT)	frvB	putative PTS enzyme, IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base T (32/34);  new base A (13/3);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 2.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCATCGCA  >  NC_000913/4090957‑4091430                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     cggcgAAACCACAATCCTTCCAGGACGATAAGGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGAAAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGTGCCAGACGTTTGATCCCG‑AAGGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAg                                                                                                                                                                                                                                                    <  2:603093/238‑1 (MQ=255)  ggcgTAACCCCAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGCCGTTTGATTCCG‑AAGGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGc                                                                                                                                                                                                                                                   <  1:1034692/238‑1 (MQ=255)     gTACCCCCAATCCTTCCAGGAGAATAATGATTGAAATGTTCCCCCCGCGGCCAACCGCCAGCCCCAAAAAAAACGAACCCCCCGCGGACACCAGCCCGATCGGCGGTTCAAAAATCCCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGACCACCAGCA‑CGCCGGAAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcggt                                                                                                                                                                                                                                               <  2:993940/239‑1 (MQ=255)      tagtcacagcccgtgcAGGAGGATAAGAAATGACATGTGCAGCGCGCAGTCAATCACCAGCACCACAAAAAACGACCCCGCCGGCGACTTCAGGCCGACCAGCGGGTCAAAGATCCCCAACCCA‑GGTGCCAGGCGTGGGACCCAG‑AACGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcgctt                                                                                                                                                                                                                                              <  2:462007/224‑1 (MQ=255)      tAACCACAATCCTTTCAGGACGAAAAGGAAGGAAATGTTAAGCGCGAGGCAAACCGCCAGCACCAGATAAAACGACCCCACCGGCGACATCAGCCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGGGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcggt                                                                                                                                                                                                                                               <  2:360662/238‑1 (MQ=255)      gaacccgaatcctcgagacacaaTAATGAAAAAAAGGTTCAGCGCCAGCCACACCGCCAGCACCAGATAAAACGACCCCACGGGCACCATCAGCCCGAGACGCGGGCCAAATATCCACAAACAA‑GGTGCCCGCCTTTTGAGTCCG‑AAAGCGACCACCAGCA‑CCCTGGAAAGCCTGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCcggt                                                                                                                                                                                                                                               <  2:950335/216‑1 (MQ=255)             accctgtccatgagtataataAATAAAATTCTCACCCCGAGGCAAATCCCCGCCACCAGATAAAACGACCCCACCGTCTACTCCCTGCCACTCAGCGGGTAAAAGTTACCCAAACCA‑GGTTCCAGACGTTTGATCCCG‑AAAGCGATCCCCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGc                                                                                                                                                                                                                                       <  2:424796/224‑1 (MQ=255)             aaTCCTTCCAGGACGTAAATGAAGAAAATGTTCAGCGGGGGGCCAATCCCCAGCACCAAATAAAACGAACCCACCGGCGAAAACCGGCCGATCCGCGGGTAAAAATCACCCAAACCA‑GGTCCCGGCCGTTGGATTCCG‑AAAGCGATCCCCGGCA‑CGCCGTTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGc                                                                                                                                                                                                                                       <  2:1127668/239‑1 (MQ=255)              aTCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAC‑GGAGCTGCCCGGGGGGAA‑GGAAAAACcgggtcgggttgg                                                                                                                                                                                                                                      >  2:86710/1‑234 (MQ=255)                  tttCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGCAATCATCGGCAAC‑AGGACTGCCAGCGCGTAA‑GGAAAAGCCGATTCTGTTGCG‑GCtg                                                                                                                                                                                                                                 >  2:761677/1‑239 (MQ=255)                    tCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGCGCCGCCCGAGCGGAA‑GGAATAAACAGTTCGGGTgaggccgtg                                                                                                                                                                                                                                >  2:507642/1‑231 (MQ=255)                          cGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCAcc                                                                                                                                                                                                                         <  2:130620/238‑1 (MQ=255)                           gATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAA‑CGAGCTGCCAGGGGGCAA‑GGAAGAGCCGGGTCGGCTGCG‑AGTGG‑GGCACCg                                                                                                                                                                                                                        >  1:1043625/1‑238 (MQ=255)                           gATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATACTCGGCAAC‑GGAGGTGCCCGAGCGGAA‑GGAAAAGCCGGTTCGGTTGCG‑GCCTT‑GGCCCCg                                                                                                                                                                                                                        >  1:1062046/1‑238 (MQ=255)                             tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGc                                                                                                                                                                                                                                                   >  1:153950/1‑211 (MQ=255)                             tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGGCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATAAACCGCAAC‑GGAGACGCCGGAGCGTAA‑GGAATAGCCGGTTCGGGTGCG‑GCAGT‑gggcccagac                                                                                                                                                                                                                     >  2:406278/1‑231 (MQ=255)                               atgaatgaAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACcac                                                                                                                                                                                                                   <  1:1058207/239‑1 (MQ=255)                               agaaaagaaattcacAGCGAAAGGCCAGTCCCCAGCACCAAATAAAGCGAACCAGCCGGCACAAACAGCCCGAACAGCGGGTCAAACATACCAAAACCA‑GGGGCCAGCCGTTTGTTCCCA‑AAAGCGACCCCCACCA‑CGCCGGTAAGGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACcac                                                                                                                                                                                                                   <  2:935692/225‑1 (MQ=255)                                   atgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATg                                                                                                                                                                                                               <  1:897649/239‑1 (MQ=255)                                      aaaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTAAAAGATCCCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATgg                                                                                                                                                                                                              <  1:1155817/237‑1 (MQ=255)                                       aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAA‑GGAGCTGCCCGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCa                                                                                                                                                                                                            >  1:431799/1‑238 (MQ=255)                                       aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGAAATCATCGGCAAC‑GGAGCTGCCCGAGCGGAA‑GGAAAAGCCGGGTTGGGTGGG‑ACTGT‑GGGGCCCACcacacatgga                                                                                                                                                                                                            >  1:512837/1‑232 (MQ=255)                                       aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCACGCCAGCGTATTGGCGGCACGCATCCGCGAC‑CGAGCTGACCGAGCGGAAGGGAAAACCCGGTT‑GGGTGCG‑ACTGG‑GGCGCCGGCCACACGGGCa                                                                                                                                                                                                            >  2:269630/1‑238 (MQ=255)                                          gTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGCAATCATCGGCAAA‑GGGGCCGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAAAGGCacta                                                                                                                                                                                                         >  1:856099/1‑238 (MQ=255)                                            tCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAAACATCGGCAAA‑GGAGCTGCCCGGGCGTAG‑GGAAAAGCCGGGTGGGTTGGG‑GCCGT‑GGCACCGGACAAAATgggccaaaag                                                                                                                                                                                                      >  1:1097077/1‑231 (MQ=255)                                             cAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCAGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTTCG‑ACTGG‑GGCCCCGAACACAATGGGACCACTg                                                                                                                                                                                                      >  1:1323707/1‑238 (MQ=255)                                              aGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAAACATCGGCAAC‑GGAGCTGCCCGCGCGGAA‑GGAATAGCCGGTTCGGGTGGG‑GCTGT‑GGCACCGACCACAAAGGCACTACCgg                                                                                                                                                                                                     >  2:278101/1‑238 (MQ=255)                                                cgcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCCAC‑GGAGCGGCCAGAGCGTAA‑GGAAGAACCGGGTCGGGTGGG‑ACCGT‑GGGACCGGCCCCCAAGGCCCTCCTGGCa                                                                                                                                                                                                   >  2:754034/1‑238 (MQ=255)                                                cgcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCAC‑GGAGCTGCCAGAGCGGAA‑GGAAAAACCGGGTCGGGTGCG‑GCCGG‑GGGCCCGCACACAAAGGCACCACTGGCa                                                                                                                                                                                                   >  2:1155817/1‑238 (MQ=255)                                                 gcaagccaaaTCGCCAGCCCCAGAAAAAACGCACCCCCCGGCGACAACAGGCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGACCACCAGCC‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAg                                                                                                                                                                                                  <  2:1300141/230‑1 (MQ=255)                                                     ggCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAA‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGGG‑ACTGT‑GGCACCGACCACACAGGGACTACTgggcgccact                                                                                                                                                                                             >  1:1168749/1‑231 (MQ=255)                                                            cgcaagacccaaaaaaaaCGAACCCCCCGGCGACATCAGGCCAACCAGCGGGTCAAAGATACCAAAACAA‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGCTCACAAGCC‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCt                                                                                                                                                                                      <  1:1253804/225‑1 (MQ=255)                                                            cGCCAGCACCAGATAAAACGAACCCGCCGGCGACATCAGGCCGACCAGCGGGTCAAAGACACCCAAACCA‑GGTGCCAGACTTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCt                                                                                                                                                                                      <  2:1054994/239‑1 (MQ=255)                                                              ccacccccAGATAAAACGACCCCACCGGGGACATCGGGCCGACCGGGGGGTCAAAGATCCCCAACCCA‑GTTGCCAGACGTTTGATCCCA‑AAAGCGACCCCCACCA‑CGCCGTTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGt                                                                                                                                                                                    <  2:512837/233‑1 (MQ=255)                                                               cAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAAAGGCACCACTGGCAGCCTCTTTTTCCTGTc                                                                                                                                                                                   >  1:280667/1‑239 (MQ=255)                                                                aGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCCGAGCGGAA‑GGAATAGCCGGTTTGGGTGCG‑ACTGG‑GGCACCGACCCCAATGGGACTACACGCAGCCTCTTTTTcccttcg                                                                                                                                                                                  >  2:175343/1‑234 (MQ=255)                                                                 gCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATTCTCCGCCAC‑GGAGCTGCCAGAGCGGAA‑AGGATAACCGGGTCGGTTGCG‑ACTGG‑GGCAACGGCCACAAAGGCACTACTGGCAACCTCTTTTTCCTGTCgg                                                                                                                                                                                 >  2:594096/1‑239 (MQ=255)                                                                   acaaGAAAAAACAAACCCACCGGCGACATCAGGCCAATCGGCGGGTAAAAGAT‑CCCAAACCAGGGTGCCAGACGTTTGATCCCG‑AAAGCGATCCCCAGCA‑CGCGGGTAAGGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCg                                                                                                                                                                                  <  2:569369/233‑1 (MQ=255)                                                                    ccAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTa                                                                                                                                                                              <  1:603688/239‑1 (MQ=255)                                                                       gATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAC‑GGAGCTGCCAGAGCGGAA‑GGAAAAACCGGTTCGGTTGCG‑ACTGT‑GGCACCGCCCACCATGGCACTAATGGCAGCCTCCTTTTTCTGTCGGGTAAAc                                                                                                                                                                           >  1:670224/1‑239 (MQ=255)                                                                       caaaaaaCGAACCCCCCGGAGACAGCAGGCCGCACAGGGGGTAAAAGATCCCCAACCCG‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                                                                                                                                                           <  2:1062046/236‑1 (MQ=255)                                                                         tAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                                                                                                                                                                           <  1:618769/237‑1 (MQ=255)                                                                                aaCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCAC‑GGGGCTGCCAGGGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCGCCGACCACCAAGGCACCACTCGCCGCCCCCTTTTTCTGTCTGGTAAAAAGT‑TTCGgc                                                                                                                                                                 >  2:314843/1‑239 (MQ=255)                                                                                 aCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCCGCGTATTGGCGGCAATCATCGGCCACGGGACCTG‑CAGGGCGGAA‑GGAATAGGCCGGTCGGTGGGGCCCGGT‑GGC‑CCCACCCCAAAGGGCCTACCGGCAGCCCCTTTTTCCTGTCgggggagctgtttgagc                                                                                                                                                                  >  2:1283039/1‑224 (MQ=255)                                                                                 aCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGgc                                                                                                                                                                 >  1:368111/1‑238 (MQ=255)                                                                                      ccGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAA‑GGAGCTGCCCGAGCGTAA‑GGAAAAACCGGGTCGGTTGCG‑ACTGT‑GGGACCGACCACAATGGGAACACTGGCGGCCTCTTTTTTCTGTCGGGGAAACAGA‑TTTGGCGCAATg                                                                                                                                                           >  2:529782/1‑239 (MQ=255)                                                                                        ggCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGaa                                                                                                                                                         <  1:86710/239‑1 (MQ=255)                                                                                           gACATCAGGCCGACCAGCGGGCCAAAGATACCCAACCCG‑GGTGCCAGAGGTTTGATCCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑g                                                                                                                                                      <  2:724574/238‑1 (MQ=255)                                                                                             cATCAGGCCGATCAGCGGGTAAAAGATCCCCAACCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGAc                                                                                                                                                                                                                      <  2:397133/174‑1 (MQ=255)                                                                                               tCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGGAAC‑GGAACTGCCCGAGGGGAA‑GGAATAACCGGGTCGGTTGCG‑ACAGTGGGCACC‑ACCACCAAGGACCAACCGGCAGCCCCTTTTTTCTGTACGGTGAACAGTGTTggggccaagtaatccgc                                                                                                                                                    >  2:431288/1‑224 (MQ=255)                                                                                                      caccggggggTAAAAGAT‑CCCAAAGCC‑GGTCTCAGGTCTTTTATTCCGAAAAGCG‑CCCCCAGCCCCGCCGTTAATCCCGCCCGCCGGCGTATTGGCGGTAATCATGGGCAAC‑GGAGCTCCCAGAGCGTAA‑GAAATAGCCGGTTCGTTTGCG‑ACTGT‑GGCCCCGACCACAATGGCACTCCTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TGGGGCGCAATGAAC‑GTCGCAA‑GACccg                                                                                                                                         <  2:1291617/232‑1 (MQ=255)                                                                                                       aTCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAA‑CGAGCTGCCAGAGCGGAA‑GGAATAGCCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGGCCCTTTTTTCCGTCGGGGAAACAGG‑TTTGGCGCAATGAAC‑ATCCCAA‑GAaccc                                                                                                                                         >  2:742344/1‑237 (MQ=255)                                                                                                       aTCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCa                                                                                                                                                                                                            >  1:397133/1‑174 (MQ=255)                                                                                                            cGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTTGGCGCAATGAAC‑GTCGCAA‑AACCCGCcgcg                                                                                                                                    >  1:938951/1‑238 (MQ=255)                                                                                                              ggTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATTCTCCGCAAC‑GGAGCCGCCCAAGCGTAA‑GGGAAAGACGGTTCCGCTGCG‑ACCGC‑GGCACCGACCCCAAATGCACCACCGGCAGCCTCTTTCTTCTGTCCGGTAAAACGA‑TCGGGCGCCATGACC‑GTCGCAC‑TACCcgccgcccac                                                                                                                                 >  2:1060536/1‑235 (MQ=255)                                                                                                                         cccAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCAC‑GGAGCTGCCCGAGCGGAA‑GGAAAAGCCGGTTCGGGTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACCTGCAGCCCCTTTTTTCTGTTCGGTGAACAGG‑TTTGGCGCAAAGAAC‑GGCGCCA‑GACCCCCCCCGTCgggggggctga                                                                                                                       >  2:1039856/1‑233 (MQ=255)                                                                                                                              aCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGG‑TTTGGCGCCATGAAC‑GTCGCCA‑GACCCGCCGCGACGGGCGGCATCAGcgcg                                                                                                                  >  1:23870/1‑238 (MQ=255)                                                                                                                              aCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGGCGGTTCGGGTGCG‑ACTGT‑GGGACCGACCACAATGGCACTACTGGCAGCCTCCTTTTTCTGTCGGGGAAACAAG‑TTTGGCGCAAATAAC‑CGCGCCA‑AGCCCGGCGCGACGGGCGGCagacggcgg                                                                                                                  >  2:65039/1‑230 (MQ=255)                                                                                                                                                tGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAAAAACCGGGTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCCCTTTTTTCTGTCGGGGAAACAGT‑TTGGGCGCAACGAAC‑GGCGCCA‑AACCCGCCCCGGCGGGCGGCCTCAGGCCGACAAAGcccgcgcagggg                                                                                                >  2:1145576/1‑229 (MQ=255)                                                                                                                                                       g‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGT‑TTGGGCGCAATGAAC‑GTCGCCA‑GACCCCCCCCGACGGGCGGCCTCCGCGCGACCACGCCGCCCATGGCGTACCac                                                                                          >  2:68690/1‑237 (MQ=255)                                                                                                                                                         aagggcacccccAGCC‑CGGCGGAAGGGCCGCCCGCCAGCGTATTGGGGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                                                                        <  1:68690/229‑1 (MQ=255)                                                                                                                                                         aaaGCGACCCCCGGCC‑CGCGGGTAGGCCCGCCCGCCAGCGTATGGGCGGTAATCACCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCGGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TGGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                                                                        <  1:622097/239‑1 (MQ=255)                                                                                                                                                              gATCCCGAGCA‑CGCGGGTAATGCCCCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATaaa                                                                                   <  1:742344/239‑1 (MQ=255)                                                                                                                                                                       cA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCCGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGG‑TTGGGCGCAATGAAA‑GGCGCAA‑AACCCGCCCCGGCGGGCGGCAACACGGCGCACACCCCGCCCACGGCGGACCAGCCATAAAAGAGttttt                                                                          >  2:1257764/1‑239 (MQ=255)                                                                                                                                                                         acgcggtaaaggccgccCGCCAGCGTTTTGGGGGTAAGCAGCGGCAAC‑GGGGCTGCCAGGGGTTAA‑GAAGTAGCGGGTGCGGTTGCG‑AGTGG‑GGCACCGCCCAAAATGGCCATACTGGCAGCCTCTTTTCCCTGTGGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTc                                                                         <  2:1142804/228‑1 (MQ=255)                                                                                                                                                                             cGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTTCTGTCGGGGAAAAAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc                                                                     >  1:342983/1‑239 (MQ=255)                                                                                                                                                                                       gcccgccAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGGACCAGTCATAAATGTGTTTTTTCAGCAGTGAGAAGc                                                           >  1:349611/1‑239 (MQ=255)                                                                                                                                                                                        cccgccAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCCCAATGGCACTACTGGCAGCCTCTTTTTCCTTTCGGGGAAACCGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCCTAAATGTGTTTTTCCAGCAGTGCGAAGCa                                                          >  1:354151/1‑239 (MQ=255)                                                                                                                                                                                         ccgcgggggTATGGGGGGAAACCGTCGGCAAC‑GGGCCTGCCAGGGCGTAA‑GGAATGGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCCCTACTGGCAGCCTTTTTTTCCTGTCGGGAAAACAGT‑TTGGGCGAAATGAAC‑GCCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAGGCag                                                         <  2:670224/231‑1 (MQ=255)                                                                                                                                                                                          cgccAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCCGAGCGGAA‑GGGAAAGCCGGTTCGGTTGCG‑ACCGT‑GGCACCGAACACAATGGCACTACTGGCCGCCTCTTTTTCCTGTCGGGGAAACAGG‑TTGGGGGCAAAGAAA‑GTCGCAA‑AACCCGCCGCGGCGGGGGGGCTTCGAGCGGCAACGCCGGCCGTGGCCGAACAGGCATAAAAGGGTGTTTCccgcaagggggaggagg                                                        >  2:841083/1‑227 (MQ=255)                                                                                                                                                                                                  ttttGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGAAA‑GAAATAGCCGGTTCGTTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCa                                                 <  1:1232954/236‑1 (MQ=255)                                                                                                                                                                                                   tttcgggtaaagcatcGGCAAC‑GGAGGTGCCAGTGCGTAG‑GGAAGGGCCGTTGCGTCGGCG‑CCTGG‑GGGCCCGACCAAAATGGCACTACTGGCGCACTTTTTTTCCGGCGGTGGAACCGGT‑TTGGCCGCATATAGC‑G‑GGCAACGACCCGCCGCGACGGGCGGCATCAGCGCGACAACTCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAGCCAgg                                               <  2:1043625/227‑1 (MQ=255)                                                                                                                                                                                                   agtGGCGGAAATCACCGGCAAG‑GGGGCTGCCAGGGCGGAA‑GAAAAGGCGGGTTCGGTGGCG‑ACTGT‑GCCCCCACCCAAAAGGGCCCTACGGGCGCCTTCTTTTCCCTGTGGGAAAAACAGT‑TGGGCCGAAATGAAC‑GTCGCAA‑GCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg                                               <  2:288620/237‑1 (MQ=255)                                                                                                                                                                                                   aTTGGCGGTAATCATCGGCAAC‑GGGGCTGCCAGAGCGTAA‑GGAATGGCGGGTTCGGTTGCG‑ACTGT‑GGCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg                                               <  1:754034/239‑1 (MQ=255)                                                                                                                                                                                                   aTTGGCGGTAATCACCGCCAAC‑GGAGCGGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTGGCG‑ACGGT‑GGCACCGACCACAATGGCACTACTGGCGCCCTCTTTTTCCTGTCGGATAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg                                               <  1:314843/239‑1 (MQ=255)                                                                                                                                                                                                    tttagctgtaccaatcggacggggAGTTGCCAGAGGATAA‑GGAATAGCCGTGGCTGTGGCG‑CGTGT‑GGCACCGACCACAATGGAACTACTGGCACCCTCTTTTTCCTGTGGGGAAAACAGT‑TGGGGCGCAATGAAC‑GTCACAG‑TGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGATCCAgg                                               <  2:469573/217‑1 (MQ=255)                                                                                                                                                                                                           gaaTCACCGGCAAC‑GGAGCGGCAAGAGCGGAG‑AGAAAAGCCGGTTCGGTGGCG‑ACTGT‑GGCACCGACCACAAGGCCACTTGTGGCAGCCTCTTTTCCCGGTCGGGTAAACAGT‑TTGGGCG‑AAAGAACGGCCGCAA‑GCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGtttt                                        <  2:703682/237‑1 (MQ=255)                                                                                                                                                                                                               cctcGGCACG‑GGAGCTGCAAGAGGGTAA‑GGAAGAGCGGGTGCGGTGGCG‑ACGGC‑GGCCCCGACCACAAGGGCACTACTGGCAGCCTCTTTTTCCTGCCGGTAAAACAGT‑TTGGGCGCAAGGACC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGa                                   <  2:1304864/237‑1 (MQ=255)                                                                                                                                                                                                                 tcGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTTGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATAGCGTCCCCGTCATCAATGTGTTTTTCCAGCAGTGTGAAGCCGAGGCACCAGGCGGATTTCTTGC‑Tg                                >  2:603339/1‑239 (MQ=255)                                                                                                                                                                                                                 tcGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑Tg                                >  1:1194718/1‑239 (MQ=255)                                                                                                                                                                                                                            aGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGCTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTCTTGATTGGGgccgcc                        >  2:528106/1‑238 (MQ=255)                                                                                                                                                                                                                                gCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGCCGGGCGGCATCAGCGCGACAACGCCGAAGATGGCGCACCAGTCATAAATGTGTTTTTCAAGCAGTGAGAAGCAGAAGAACCAGGCGGGTTTATTGT‑TGGGGCCCGCCATATc                  >  2:1043736/1‑239 (MQ=255)                                                                                                                                                                                                                                       cgaaa‑GGAAAAGCCGGTTCGGTTGCG‑ACGGG‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGAAA‑GCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGc            <  1:1012662/235‑1 (MQ=255)                                                                                                                                                                                                                                           a‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAgaagaa                                                    >  1:607227/1‑195 (MQ=255)                                                                                                                                                                                                                                             ggAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCa       >  2:1094124/1‑238 (MQ=255)                                                                                                                                                                                                                                                aTAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGGCCCTAACGGACCCCCGccatcc   >  2:1007969/1‑234 (MQ=255)                                                                                                                                                                                                                                                 tAGCCGGTTCGGTTGGG‑ATTGT‑GGCACCGACCAAAATGGCACTACTGGCCGCCTCTTTTTCCTGGCGGGAAAACAGT‑TTGGGCGCAATGAAC‑GCCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCATCGCa  <  1:295941/239‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAC‑GGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCG‑ACTGT‑GGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT‑TTGGGCGCAATGAAC‑GTCGCAA‑GACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCATCGCA  >  NC_000913/4090957‑4091430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑