Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,151,328	T→G	22.7%	A590A (GCA→GCC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,151,328	0	T	G	22.7%	132.3 / 4.2	91	A590A (GCA→GCC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base T (46/17);  new base G (10/15);  total (57/34)
Fisher's exact test for biased strand distribution p-value = 6.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCGTTGTTC  >  NC_000913/4151091‑4151565                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gctggaGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAACAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAT‑CAGCGCCGCATGA‑GC‑AGGTg                                                                                                                                                                                                                                                <  1:427222/234‑1 (MQ=255) gcgGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTg                                                                                                                                                                                                                                                <  2:416556/239‑1 (MQ=255)    gTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCCCATGA‑GC‑AGGTg‑cg                                                                                                                                                                                                                                             >  1:1244980/1‑238 (MQ=255)           tCGCAGGAGGTGACTGACAGTTCATCCATAATGCTCCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCcgcg                                                                                                                                                                                                                                     <  2:1250838/239‑1 (MQ=255)           tCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCcgcg                                                                                                                                                                                                                                     <  2:502615/239‑1 (MQ=255)               aGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCCTGA‑GC‑AGGGG‑GCGCGCCGCGACCa                                                                                                                                                                                                                                 >  1:281225/1‑239 (MQ=255)               aGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGAccc                                                                                                                                                                                                                                 >  1:1071122/1‑238 (MQ=255)                    aTGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑CGGTG‑CGCCGCCGCGGCCAATggg                                                                                                                                                                                                                            >  1:2955/1‑238 (MQ=255)                      gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATTGCCCCGGCATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGTGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCCTGA‑GC‑AGGTG‑CGCCGCCGCGGCCTATGG‑aa                                                                                                                                                                                                                          >  1:594836/1‑238 (MQ=255)                           acAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATTA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AAccgcc                                                                                                                                                                                                                     >  1:487070/1‑238 (MQ=255)                             aGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGCCGGTTGTGACCA‑CCACGCCCGACGACGC‑A‑GTG‑CGCCGCCGCGCCCACTGG‑AAAcgccgc                                                                                                                                                                                                                   >  2:764245/1‑238 (MQ=255)                                tCATCCAAAAGGCGCCGCCAGCTTTTTGCCGGCGCGGGGGGTGGCAGCAGGTTGGTTT‑CCAGAACCGCCCCGGTAAAAAGCG‑ACGGGCGGCTGACGGTGATTTCGGGCAGACCATATTAAAAGCGG‑ATCTC‑CCCCCCTTGTGCGGTTACGCGCGGGCCGCC‑TTTCGGG‑CTTCGGGGCGGTTGTGCCAG‑CGGCGCCGCAGGA‑GC‑GGGTG‑CCCCGCCGCGACCAATGG‑AACCGCCGCGAcc                                                                                                                                                                                                               <  2:1108863/239‑1 (MQ=255)                                       tAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGGG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGaac                                                                                                                                                                                                        >  2:1122938/1‑239 (MQ=255)                                         aTGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGTCGGCCCCC‑TTTCCGG‑CTTCCTTGCGGCTTGGCCAG‑CAGCGCCGCACTT‑CC‑CGGGG‑CGCCGCCTGGGCCCAAGG‑GACCGCCGCGCCCGCgggccaa                                                                                                                                                                                                      >  2:727116/1‑234 (MQ=255)                                         aTGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGAGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGGCCCATGG‑AACCGCCGCGACCGGGGcacaa                                                                                                                                                                                                      >  1:145426/1‑239 (MQ=255)                                             gACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCCCCCCGACCGTGGAACAACGTc                                                                                                                                                                                                  >  1:786985/1‑239 (MQ=255)                                              aCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGGATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGGTGTGACAA‑GAGCGCCGCATGA‑GC‑AGGTG‑CGCCCCCGCGCACAAAGG‑GACCCCCGCGACCCGGGAAAAACGACa                                                                                                                                                                                                 >  2:610449/1‑239 (MQ=255)                                               cGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGGG‑CGCCGCCGCGACCAATGG‑AACCGCCCCGCCCGTGGAACAACGTCa                                                                                                                                                                                                 >  1:1108543/1‑238 (MQ=255)                                               cGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGGTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGGTGTGGCAG‑CAGCGCCCCCTGA‑GC‑AGGTG‑CGCCGCCCGGACCAATGG‑CACCGCCGCGACCGTGGTACAACggcc                                                                                                                                                                                                 >  2:96814/1‑235 (MQ=255)                                                 ccAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGGG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAAAAAACTCAGc                                                                                                                                                                                               >  1:1216143/1‑238 (MQ=255)                                                        tctTGCGGCGCGGGCGGGGGCGGCAGGTTGGCTT‑CCAGAACCCCCCGGGTATAAAGCG‑CGAGGCTGCTGCGGGTTTTTCGTGGCAGACTATTTTAAAATCGG‑TCCAT‑CTCCCCCTTTTCGGTCCCGGGCGGGCCGCC‑TTTCCGG‑CTTCCTGGGGGTTGTGACAG‑CAGCCCCGCATGA‑GC‑AGGGG‑CGCCGCCGCGACAAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATAcc                                                                                                                                                                                       <  2:832295/239‑1 (MQ=255)                                                          tttCGGCTCCGGCGGTGGCAGCAGGTTTGCTT‑CCAGAATCTCCCCGGTATAAAGCG‑GCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑CTCAT‑CTCGCCCTGCTCGGGTACGCCCAGGCCGCC‑TTTCAGG‑CTTACTGGCGGCTTTGACAG‑GCTAGCCGCACGA‑GG‑CGGGG‑CCCCGCCCCCAACAAACG‑AACCGCCGCGGACGAGGaacaactggccgccaaaacac                                                                                                                                                                                      >  2:67077/1‑223 (MQ=255)                                                               gCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCCTGA‑GC‑AGGGG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGGGGCACAACGGTCGCTCAATACCCGCtttt                                                                                                                                                                                >  1:159460/1‑239 (MQ=255)                                                               gCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGG‑GC‑AGGGG‑CGCCGCCGCGACCCATGG‑AACCGCCGCGACCGTGGGACAACGTCAGGTCAATACCCGCtttt                                                                                                                                                                                >  1:724406/1‑239 (MQ=255)                                                               gCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCCCGCCCGCGGAACAACGTCAGCTCAATACCCGCtttt                                                                                                                                                                                >  1:992046/1‑239 (MQ=255)                                                                   gggggggggcggcGGGTGGGCTC‑CCAAAATCGCCCCGGAAAAAAGCG‑ACGGGGTGCTGACGGTGTTTTCGGGCAGACCATTTTAAAAGCGG‑TTCAT‑CTCCCCCTGTTCGGTTACGCGCGGGCCGCC‑TTTCAGG‑CTTCCGGGCGGTTGTGCCAG‑CGGCGCCGCGGGA‑GC‑GGGTG‑CGCCGCCGCGACCAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCg                                                                                                                                                                             <  2:272977/231‑1 (MQ=255)                                                                   gggggggggcggcAGGTGGGCTT‑CCAAAATCGCCCCGGTATAACGCG‑ACGGGCTGTGGCGGGTGTTTTCTGGCAGCCCATATTAAAAGCGG‑ATCTC‑CCCGCCCTTTGCGGTTACGCGGAGCCCGCT‑TTCCAGG‑TTTCCGGGGGGTTGTGAAAG‑CAGCCCCGCATGG‑GC‑AGGGG‑CGCCGCCGCGACAAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGc                                                                                                                                                                            <  2:1165494/232‑1 (MQ=255)                                                                    gggggggggaggagGTTGGCTTCCCAGACCCCCCCCGG‑ATAAAGGGCCCGGGCTGCTGCGGGTGATTTGGGGCAGCACTTATTT‑AAGCGG‑ATCCTCCCCGCCTGGTTCGTTTCCGCGCGGGCCGCC‑TTCCGGG‑CTTCCTGGGGGTTGGGCAGG‑CAGCCCCGCATGG‑GC‑AGGGG‑CGCCGCCGCGACCAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGc                                                                                                                                                                            <  2:774967/227‑1 (MQ=255)                                                                    ggcggTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGGTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGCCCAAGGG‑AACCGCCCCGACCGGGGGACAACGCCCGCTCAATACCCCCCTTTTCGCa                                                                                                                                                                           >  2:939735/1‑239 (MQ=255)                                                                    ggcggTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGGGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCCTGG‑GC‑AGGGG‑CGCCGCCGCGACCAACGG‑AACCGCCGCCGCCGGGGGAACACGTCAGCTCAATACCCGCTTTTTccgc                                                                                                                                                                           >  2:944214/1‑236 (MQ=255)                                                                                        cttgcagaggcgccgggttataGAGCG‑GCGGGCTGGGGGCGGTGTTTTGGGGCAGACAATTTTTAAGGCGG‑ATCCT‑CTCGCCTTGTTCGTTTCCGCGCGGGCCGCT‑TTGCAGG‑CTCCCGGGGGTTTGGGACAG‑CAGCGCCGCAGGA‑GG‑GGGGG‑CGCCGCCGCGCCCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATc                                                                                                                                                         <  2:1244980/220‑1 (MQ=255)                                                                                        ctcccaaaaccccccgGGTATAAAGCA‑ACGGGCTGTGGGGGTTGTTTTCTGGAAGACCTTTTTAAAAGCGG‑ACCTT‑CTCCCCCTTTGGGGTTAGGCGCGGGCCGTC‑TTCCGGG‑CTTCCGGGGGTTTGTGAAAG‑CGGCCCCGAATGG‑GC‑AGGTG‑CGCCGCCGCGCCAAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATc                                                                                                                                                         <  2:1216143/226‑1 (MQ=255)                                                                                         tgccagacgccccccGGTATAAAGCG‑ACGGGTGTGTGACGGTGGTTTTGGGCAGACCATTTTAAAAGCGG‑TTCAT‑CTCGCCCGGTTCGGTTCCGCGCGGGCCGCC‑TTTCAGG‑CTTCTTGGCGGTTGTGACGG‑CAGCCCCGCAGGA‑GC‑GGGTG‑CCCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCt                                                                                                                                                       <  2:992046/228‑1 (MQ=255)                                                                                         tgccaacacctcccgtgTAAAAAGGG‑ACGGGCTGCGGACGGGTGTTTCGGACAGACCTTTTTTAAAGCGA‑ATCCC‑CCCGCCTTTTTGGGTTCCCGCCCGCCCGCT‑TTGCAGG‑CTTCGGGGCGTTTTGGCCAC‑CGGCGCCGCATGA‑GC‑GGGGG‑CCCCGCCGC‑ACCAAAGGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCt                                                                                                                                                       <  2:739785/223‑1 (MQ=255)                                                                                           ccAGACCCGCCCCGTAAAAAAGGG‑ACAGGCTGCTGGCGGTTATTTCGGGCAGACCATTTTTAAGGCGG‑ATCAC‑CCCCCCTTGTTGGGTTCCGCGCGGGCCGCT‑TTTCAGG‑CTTCCGGGGGGTTGGGAAAG‑CAGCCCCGCAGGG‑GG‑GGGGG‑CGCCCCCGCGACCAAGGA‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCtgt                                                                                                                                                     <  2:159460/239‑1 (MQ=255)                                                                                                      ccGGAATAAAGCG‑ACAGGCTGCTGGGGGTGTTTGCTGGCAGACAATTTTTAAAGCGT‑ATCAC‑CGCGCCTGGTTCGGTTACGCGCAGGCCCCT‑TTTCAGG‑CTCCCTGGGGGTTGTGAAAG‑CAGCGCCGCATGA‑GC‑GGGCG‑CGCCCCCGCCCCCAATGA‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTg                                                                                                                                          <  2:487070/239‑1 (MQ=255)                                                                                                               aGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAAAGCATCCTGTGCCTGATATTGCGCCCCggc                                                                                                                                 >  2:953639/1‑238 (MQ=255)                                                                                                                    aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAT‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGaa                                                                                                                                >  1:291453/1‑236 (MQ=255)                                                                                                                    aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGGCCGTGGAACAACGGCACCTCAATACCCGCTTTTTCGCAGGTTTTTATTAATGCATCCTTTGCCTGCTATTGCGCCCAGGCAGCt                                                                                                                             >  1:1272396/1‑239 (MQ=255)                                                                                                                    aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGaa                                                                                                                                >  1:975448/1‑236 (MQ=255)                                                                                                                    aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGaa                                                                                                                                >  1:1217406/1‑236 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGTCAA‑CAGCCCCGCATGA‑GC‑AGGTG‑CGACGCCGCGACCAATGG‑AACCGCCGCCACCGTGGAACAACGTACGCACAAAACCCGCTTTTTCCCGGGGTTTGATTAACGCTACCTCTGCCTGTAAGTTCGACCAAGAAGcc                                                                                                                             >  2:716331/1‑237 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑GAACGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AAACGCCGCGGCCGCGGGACAAAGTCCCCTCCATACCCGCTTTTTACCCGGATTTGATTAAAGGAATCTTTGCCTGATATTTCGCCCACGacggt                                                                                                                             >  2:974599/1‑234 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGCCCAATGG‑AACCGCCGCGCCCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTTATGCATCCTGTGCCTGCTATTTCGCCCAGGAAGCt                                                                                                                             >  2:890566/1‑238 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑GACCGCCGCGACCGTGGAACAACGCCAGCTCCATACCCGCTTTTTCGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTGCGACCAGGAAGCt                                                                                                                             >  2:555176/1‑238 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCCGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTTCGCCCCGGAAGCt                                                                                                                             >  1:742229/1‑238 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCCATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                             >  1:384538/1‑238 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTTCGCCCAGGAAGCt                                                                                                                             >  1:1255006/1‑238 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                             >  1:236754/1‑238 (MQ=255)                                                                                                                     cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGCG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGCTTTGCTTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCt                                                                                                                             >  2:113935/1‑238 (MQ=255)                                                                                                                          ggctgcggttgttctctcgcgaaCCTTATTTAATGCGA‑TCCAT‑CCCCCCCGTTTCGTTACCGCGCGGCCCGCC‑TTGCAGG‑CTTCCGGGTGGTTTGCACGG‑GATC‑CCGGAAGG‑GCTGGGCG‑CGCCGCCGCGACAAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCAt                                                                                                                        <  2:67985/216‑1 (MQ=255)                                                                                                                            ctgACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCa                                                                                                                      >  1:242862/1‑238 (MQ=255)                                                                                                                            ctgACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGGG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGGCCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTGCCCCCAGGAAGCTGCCCTCa                                                                                                                      >  1:1313431/1‑238 (MQ=255)                                                                                                                                 gtttaTTTCTGG‑AGACCCTTGTTAAAGCGGTCTCAC‑CCCGCCGTTTGGGGTTCGCCGCAGGCCGCT‑TTTCAGC‑CTTCTGGGGTGTGGTACCAG‑CAGGCCCGAATGA‑GA‑GGGTG‑CCCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCg                                                                                                                <  2:678647/235‑1 (MQ=255)                                                                                                                                   ttatttcggcaaGACCATATTAAAGGGGG‑ACCAC‑CTCGCCTTGTTCGGTTCCGCGCGGGCCGCC‑TTCGGGG‑CTTCCGGGCGGTGGGACCAG‑CAGCGCCGCAGGA‑GC‑GGGGG‑CCCCGCCGCGACCAATGA‑ACCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGc                                                                                                               <  2:236754/227‑1 (MQ=255)                                                                                                                                           ggCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCtttt                                                                                                        >  2:1352940/1‑237 (MQ=255)                                                                                                                                                aCCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTTATTAATGCCTCCTGTGCCTGATATTTCGCCCAGGAAGCTGCCATCCCCCCCCCCTCTTTTGCTTAg                                                                                                  >  2:602462/1‑238 (MQ=255)                                                                                                                                                 ccATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCCCGACCGGGGAACAACGTCAGCCCAAAACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTTCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGGGTc                                                                                                >  2:686464/1‑239 (MQ=255)                                                                                                                                                      tgttaaaggcggttcccccgccccTGTTGGGTCCCGCGGAGGCCCCC‑TTCCGGG‑CTCCCGGGGGGTTGGGACAG‑CCGCGCCGCAGGA‑GC‑GGGTG‑CCCCCCCGCGACCAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGa                                                                                             <  2:975448/218‑1 (MQ=255)                                                                                                                                                        tAAAGGGG‑ATCGT‑CTCGCCCTGTGCGATTGCGCGGCGGCCCCC‑TTCGGGG‑CTGCGGGGCGGTGGAGAACG‑GAGCCCCGCGTGG‑GA‑GGGGG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAg                                                                                         <  2:1108543/239‑1 (MQ=255)                                                                                                                                                         aaaGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGGGCCTGATATTGCGCCCCGGAAGCTGCCCTCACTCCCGCCTCTTTTGCTGAGTCGGAATAg                                                                                         >  2:952911/1‑238 (MQ=255)                                                                                                                                                            gCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCTAGGAAGCTGCCCTCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCaa                                                                                     >  1:877379/1‑239 (MQ=255)                                                                                                                                                              gg‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATc                                                                                                                                                         >  1:198526/1‑169 (MQ=255)                                                                                                                                                               g‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                                  >  2:869555/1‑239 (MQ=255)                                                                                                                                                               g‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCCCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCCCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATTTTTTGCTGAGTCGGAATAGCCAatca                                                                                  >  1:665034/1‑239 (MQ=255)                                                                                                                                                                 atcat‑cTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCCGGAAGCTGCCATCAATCCCCCCTCTTTTGCTGAGTCGGAATAGCCAatca                                                                                  >  2:934803/1‑238 (MQ=255)                                                                                                                                                                 atcat‑cTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatca                                                                                  >  1:1071024/1‑238 (MQ=255)                                                                                                                                                                 atcat‑cTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTCGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGGGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAGTAGCCAatca                                                                                  >  1:1308944/1‑238 (MQ=255)                                                                                                                                                                  ccat‑cCCGCCTTGTGCGGTGACGCGCAGCCCTCC‑TTGAGGG‑CTCCCGGGCGGTTGTAACGG‑CGGCGCCGAAAGA‑GA‑GGGTG‑CCCCGCCGCGACAAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatcat                                                                                 <  2:613109/237‑1 (MQ=255)                                                                                                                                                                   tccccccGCCCTGTCGGGTTGCGAG‑AGGCCCCCTTTTCAGC‑GTTCCGGGGGGTGGTG‑CAGCCACCGCCACAGGG‑GC‑GGGGG‑CGCCGCGGCGACCAATGA‑ACCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatcat                                                                                 <  2:1217406/232‑1 (MQ=255)                                                                                                                                                                        tcGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCa                                                                            >  1:749878/1‑238 (MQ=255)                                                                                                                                                                            ccTGTGCGTTTGCGCCCGGGCCGCT‑TTTCAGG‑CTGCCGGGGGGTTGGCACAG‑CAGCGCCGCATGA‑GC‑GGGTG‑CGCCGCCGCGACCAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGt                                                                       <  1:151351/239‑1 (MQ=255)                                                                                                                                                                             cgggtCGGTACCGCGCGGCCCCCC‑TTTCGGC‑CTCCCGGGCGTTTGGGCCGC‑CAGCCCCGCA‑GG‑GC‑GGGGGCCGCCGCCGCGCCAAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGtt                                                                      <  2:786985/235‑1 (MQ=255)                                                                                                                                                                                ttCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTg                                                                    >  1:495444/1‑238 (MQ=255)                                                                                                                                                                                 cgggTTCCGGGCAGGCCCCT‑TTCCGGG‑CTCCCGGGGGGTTGGGACAG‑CAGGGCCGCAGGG‑GC‑GGGGG‑CGCCGCCGCGACAAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGcc                                                                  <  1:639649/237‑1 (MQ=255)                                                                                                                                                                                  tcggttcgcccccggcccc‑TTTCCGGCCTTCCTGGCGGTGGGGACAG‑CGGCCCCGCTGGG‑GC‑GGGGG‑GCCCGCCGCGCCCATTGT‑ACCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGcc                                                                  <  2:724406/222‑1 (MQ=255)                                                                                                                                                                                               gccgcc‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa                                                     >  2:1098238/1‑238 (MQ=255)                                                                                                                                                                                               gccgcc‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAACGCCGCCTGA‑GC‑AGGTG‑GGCCCCCGCGACCAATGG‑AACCGCCCCCCCCGTGGCACAACGTCAGCCCAATACCCGCCTTTTCGCCGGTTTTGATTAATGCATCCAGTGCCTGTTATTGCGCCCCGGAAGCTGCCTTCACACCCGCATCTTTTTCTGAGTCCGACTAGCCAAACATACCCCTCTTTTTGCCCTCAAACAGGccc                                                     >  2:72452/1‑237 (MQ=255)                                                                                                                                                                                                 gctgctgtccggccTTCCGGGCGGGTGGGACAG‑CGCCGCAGAAGGA‑GC‑TGGTG‑CGCCGCGCCAAACAATGA‑ACCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa                                                     <  2:742229/225‑1 (MQ=255)                                                                                                                                                                                                   tc‑TTACAGG‑CTCCCGGAGGGTTGAGACGG‑TCGCGCCGCAGGG‑GC‑AGGGG‑CGCCGCCGCGCCCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                                <  2:35549/238‑1 (MQ=255)                                                                                                                                                                                                   cc‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCCTCTGTTTGCCCTGAATCAGGCCACGAt                                                 >  1:1348369/1‑238 (MQ=255)                                                                                                                                                                                                   cc‑TTGCAGC‑CTCCCTGGGGTTTGGGACGG‑CGGCGCCGAAGGA‑GC‑AGGGG‑CGCCGCCCCCACAAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                                <  1:1132866/239‑1 (MQ=255)                                                                                                                                                                                                          aGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGTTTAATGCATCCTGTGCCTGATATTGCGCCCCGGAAGCTGCCATCACTCCCGCATCTTTTGCTGGGTCGGAATAGCCAATCATCACACTCTGTTTGCCCTTAATCAGGCCACGCTAcacgc                                           >  2:1256747/1‑234 (MQ=255)                                                                                                                                                                                                                tCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGGAGCTGCCATCACTCCCGCCTCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCCCGCTACCCGTCAatat                                     >  2:989505/1‑239 (MQ=255)                                                                                                                                                                                                                         ctgtgACAG‑CAGCGCCGCATGA‑GG‑AGGTG‑CGCCGCCGCGACCAAAGG‑GACCGCCGCGACCGTGGAACAACGGCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTGAGCCCAGGAAGCTGCCATCACTCCCGCCTCTTTTGCTGAGTCGGAATAGCAAATCATCCCCATCTGTTTTTCCACAATAAGTCCACGAAACACGTCAAAAtttacagccg                            >  2:765060/2‑231 (MQ=255)                                                                                                                                                                                                                          tgggACAG‑CGGCGCCGCATGA‑GC‑GGGTG‑CGCCGCCGCGACAAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCt                            <  1:952911/235‑1 (MQ=255)                                                                                                                                                                                                                             gACGG‑CCGCGCCGCTGGG‑GG‑AGGTC‑CGCCCCCGCGACCAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTggg                         <  2:384538/238‑1 (MQ=255)                                                                                                                                                                                                                                          gctgga‑gg‑agGGG‑GGCCGGCGCGTCCAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGt             <  1:764245/234‑1 (MQ=255)                                                                                                                                                                                                                                               a‑GC‑AGGTG‑CGCCGCCGCGACCAAGGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCg        <  1:1122938/238‑1 (MQ=255)                                                                                                                                                                                                                                                 ggcggttG‑CGCCGCCGCGACAAATGT‑ATCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCATGCCCCGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCg        <  1:72452/232‑1 (MQ=255)                                                                                                                                                                                                                                                 gC‑AGGTG‑CGCAGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACACCGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCATGTGCCTGATATTGCGCCCCGGAAGCTGCGATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACAATCTGTTTGCCCTGAATCAGGCCACGCAACCGGTCAAAATTGATCTGCAGGGTCAGGGCATCGTTGGCgtg      >  2:1138593/1‑238 (MQ=255)                                                                                                                                                                                                                                                  gtgagTG‑CGCCGCCGCTACCAAGAA‑ATCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCg        <  2:39013/233‑1 (MQ=255)                                                                                                                                                                                                                                                       tG‑CGCCGCCGCGCCAAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCGTTGTTc  <  1:602462/238‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTT‑CCAGAATCGCCCCGGTATAAAGCG‑ACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGG‑ATCAT‑CTCGCCCTGTTCGGTTACGCGCAGGCCGCC‑TTTCAGG‑CTTCCTGGCGGTTGTGACAG‑CAGCGCCGCATGA‑GC‑AGGTG‑CGCCGCCGCGACCAATGG‑AACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGGCGTTGTTC  >  NC_000913/4151091‑4151565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑