BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,412,891	T→G	11.6%	G168G (GGT→GGG)	yjfM →	DUF1190 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,412,891	0	T	G	11.6%	406.7 / 5.2	140	G168G (GGT→GGG)	yjfM	DUF1190 family protein
Reads supporting (aligned to +/- strand): ref base T (35/86); new base G (17/2); total (52/88)
Fisher's exact test for biased strand distribution p-value = 6.42e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAT  >  NC_000913/4412657‑4413127

cTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                                                                                                                                                                                                                                                <  1:499123/238‑1 (MQ=255)

         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTGCTCTTTTTCTTCGCGGCCCGTCTGGCGGAGCACTTCTTGGGGT‑TACTTCTGgc                                                                                                                                                                                                                                     >  1:377342/1‑239 (MQ=255)

            cAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTGTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCt                                                                                                                                                                                                                                  <  2:760533/239‑1 (MQ=255)

            cAAAACGGCATTTTAAGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCt                                                                                                                                                                                                                                  <  2:702798/239‑1 (MQ=255)

                    cATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTc                                                                                                                                                                                                                           <  1:324769/238‑1 (MQ=255)

                           tGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCaaaa                                                                                                                                                                                                                   <  2:81607/239‑1 (MQ=255)

                            gCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGGT‑TACTTCTGGCGCTCCCGTTCTGGCaaaaa                                                                                                                                                                                                                  >  1:297342/1‑239 (MQ=255)

                            gCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCaaaaa                                                                                                                                                                                                                  >  2:957434/1‑239 (MQ=255)

                              cGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCaaaaaa                                                                                                                                                                                                                 <  1:473056/238‑1 (MQ=255)

                                aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCCCCCCGTCTGGCGCAGCCCTTCTGGTGAT‑TACTCCTTGCGCCCCGGCTCTGGCAAAAAagag                                                                                                                                                                                                              >  2:1291910/1‑239 (MQ=255)

                                    tCCGAAGAATATGACTCAGCAGAACTTTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGAGTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCATCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TTCTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCtt                                                                                                                                                                                                          <  2:408731/239‑1 (MQ=255)

                                                gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGggcg                                                                                                                                                                                             <  1:1024809/239‑1 (MQ=255)

                                                  cTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTc                                                                                                                                                                                                                           <  2:919021/208‑1 (MQ=255)

                                                  cTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTc                                                                                                                                                                                                                           >  1:919021/1‑208 (MQ=255)

                                                  cTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGGTATCCTCTTGG‑GCTCCGGTCTCGGCAAAAAAAAGACTT‑CTC‑TTTCggcggcg                                                                                                                                                                                          >  2:917173/1‑238 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGGCTTATTC‑TTCTGGGGGCttt                                                                                                                                                                                        >  1:68287/1‑239 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGTTTCGCGCCCCGTCTGGCGCAGCACTTCCGGGGAT‑TATACCCGGCGCTCCGGCTCTGGCAAAAAAGAGGCTTACCC‑TTTGGGCGGCttt                                                                                                                                                                                        >  2:733289/1‑239 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCCGGCTGGGGCAGCACTTCTTGGGAT‑TACTTCTTGCGCTCCGGGTCTGGCAAAAAACGGGCTTAATCTTTCGggcgcttt                                                                                                                                                                                         >  1:1290941/1‑235 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCttt                                                                                                                                                                                        >  1:243222/1‑239 (MQ=255)

                                                       cagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTTCTCTTTTGCTTCGCGGCCCGTCTGGCGGAGCCCTTCTCGGGGT‑TACCTCTGGGTCTCCGGTTTAGGCCAAAAAGAGACTTATTC‑TTTCGGCGGCcttt                                                                                                                                                                                       >  2:137599/1‑237 (MQ=255)

                                                       cagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGATAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTa                                                                                                                                                                                       >  1:188750/1‑238 (MQ=255)

                                                        agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTac                                                                                                                                                                                      <  1:560577/238‑1 (MQ=255)

                                                        agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTCCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTac                                                                                                                                                                                      <  2:1207829/238‑1 (MQ=255)

                                                          aaCTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGGCTTCCTC‑TTCGGGCGGCTTTccca                                                                                                                                                                                    >  1:1008235/1‑238 (MQ=255)

                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCaaaa                                                                                                                                                                              >  1:1286267/1‑239 (MQ=255)

                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGGTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTTTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTATCACCaaaa                                                                                                                                                                              >  2:1216637/1‑239 (MQ=255)

                                                                cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCaaaa                                                                                                                                                                              <  1:30339/238‑1 (MQ=255)

                                                                    cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCCGCACTTCTGGTGGT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAAAGTCTTACTC‑TTCGGGCGGGTTTACCCACaaaaaaac                                                                                                                                                                          >  2:392448/1‑236 (MQ=255)

                                                                             aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGGTCTGGCCAAACAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGtt                                                                                                                                                               >  1:201940/1‑239 (MQ=255)

                                                                                   gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTcgc                                                                                                                                                         >  1:400698/1‑239 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTcgcg                                                                                                                                                        <  1:1234561/238‑1 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTcgcg                                                                                                                                                        <  1:1247517/238‑1 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTcgcg                                                                                                                                                        <  1:502555/238‑1 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTcgcg                                                                                                                                                        <  1:957434/238‑1 (MQ=255)

                                                                                      actacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCgcggc                                                                                                                                                      <  1:1338129/239‑1 (MQ=255)

                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TGCTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTTCCACCAAAAAAGCGT‑CTACCGTTTTTCGCCGCGGCTAt                                                                                                                                                >  1:61692/1‑239 (MQ=255)

                                                                                             cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATg                                                                                                                                               <  1:911382/239‑1 (MQ=255)

                                                                                               aTGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGt                                                                                                                                             <  2:94029/239‑1 (MQ=255)

                                                                                                    gAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGtt                                                                                                                                         <  2:803278/238‑1 (MQ=255)

                                                                                                          agTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCTTCTTTTGCTTAGCGCCCAGTCTTGCGCAACACTTCTGGGGAA‑TACACCTGGCGCTCCGGTTCTTGCCAAAAAAAGGTTTTCTC‑TTTGAGCGGCTTTTCCAACCACAAAACGTACTA‑CGTTTATCGAGGCGGGGATGGTCGTTTTGCCac                                                                                                                                  >  2:255825/1‑238 (MQ=255)

                                                                                                          agTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCTCGCCCAGTCTGGCGCAGCCCTTCTGGTGAT‑TATTCCTCGCGCTTCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGCGCGGCTTTTCCACCAAAAAAGCGT‑CTACCGTTTCTCTGCGCGGCGATTGTCGGTCTTcccg                                                                                                                                  >  1:67256/1‑237 (MQ=255)

                                                                                                          agTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTcgcg                                                                                                                                                        <  1:1253959/217‑1 (MQ=255)

                                                                                                            ttGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCCCCCCAGCTGGCGGAGCACTTCTGGTGGT‑TTCTCCTGGCGCTCCGGTTCTGGCCAAAAACAGGCTTTATC‑CTCGGGCGGCTTTACCACCACAAAAACGT‑CTAACCTTTCTCGCGGGGGCTCTTGTTGTTTTTcccaa                                                                                                                                 >  2:752257/1‑235 (MQ=255)

                                                                                                            ttGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGGT‑TACTCCTGGCGCTCCGGTTCTTGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAACGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGgtctttccga                                                                                                                                 >  1:404190/1‑233 (MQ=255)

                                                                                                            ttGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑gc                                                                                                                                >  1:830133/1‑238 (MQ=255)

                                                                                                             tGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑gcg                                                                                                                               <  1:968968/238‑1 (MQ=255)

                                                                                                                 tCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGt                                                                                                                         <  2:478470/239‑1 (MQ=255)

                                                                                                                 tCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGt                                                                                                                         <  1:915983/239‑1 (MQ=255)

                                                                                                                    cGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGGT‑TACTCCTGGCGCTCCGGGTCTTGCAAAAAAAAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CCACCGTTTCTCGCGGCGGCTATGGTCGTTCTTTCC‑ACCC‑CCGTgg                                                                                                                       >  2:583013/1‑238 (MQ=255)

                                                                                                                    cGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGGT‑TACTCCTGGCGCCCCGGTTCTGGCAAAAAAAAGACTTACTC‑TTTTGGCGGCTTTTCCACCCAAAAAGCGG‑CTACCCTTTTTCGCGGCGGCCCTGGTTGTTCTTCCC‑ACGC‑CCGCggg                                                                                                                      >  2:1121117/1‑239 (MQ=255)

                                                                                                                      gTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTTCTCTTTTGCTTCGCGCCCCGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTTGGGCGGCCTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCCATGGTCGTTCTTCCC‑ACGc‑cccgggg                                                                                                                      >  2:291831/1‑232 (MQ=255)

                                                                                                                      gTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCCGGTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCCTTTGTCGTTCTTTCC‑ACGC‑CCGTggg                                                                                                                      >  2:476193/1‑237 (MQ=255)

                                                                                                                       tCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCa                                                                                                                    <  2:150349/238‑1 (MQ=255)

                                                                                                                           ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTggg                                                                                                               <  2:488851/239‑1 (MQ=255)

                                                                                                                           ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTggg                                                                                                               <  1:733289/239‑1 (MQ=255)

                                                                                                                           ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTggg                                                                                                               <  2:400698/239‑1 (MQ=255)

                                                                                                                             gtGGATTTTTGGTAAGCCGGGTGAGTCGTAAAGAGCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCGCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑GTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGa                                                                                                             <  2:154799/238‑1 (MQ=255)

                                                                                                                                 aTTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGc                                                                                                          <  2:1166132/238‑1 (MQ=255)

                                                                                                                                    tttGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAAt                                                                                                      <  1:803053/239‑1 (MQ=255)

                                                                                                                                    tttGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAAt                                                                                                      <  1:719210/239‑1 (MQ=255)

                                                                                                                                         tAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGc                                                                                                 <  2:825333/239‑1 (MQ=255)

                                                                                                                                          aaGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTTCTCTTTTTCTTCGCCCCCAGTCTGGGGCCGGACTTCTGGGGGT‑TTCTCCTGGCGCTCCCGTTCTGGCAAAAAAGAGTCTTTCTC‑TTTGGGGGGCTTTACCACCACAAAACGGA‑CTACCCGTTTTCCCGGCGGGTCAGGTCGTTCTTCCA‑CCGCACCGCGGGCTTTGGGGGGGcgacaaggca                                                                                                 >  2:252496/1‑230 (MQ=255)

                                                                                                                                           aGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTg                                                                                               <  1:476193/239‑1 (MQ=255)

                                                                                                                                              cGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTgaga                                                                                            <  2:20202/239‑1 (MQ=255)

                                                                                                                                                   ttttCGTAAAGATCGCGATGGGCCGTTTGTGTATAACCGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACaa                                                                                       <  2:1149533/237‑1 (MQ=255)

                                                                                                                                                   tATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGAcaca                                                                                        <  1:255825/238‑1 (MQ=255)

                                                                                                                                                               tCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCg                                                                           <  1:90534/239‑1 (MQ=255)

                                                                                                                                                                  cgATGAGCCGTTTGTTTATAACAGCGGCGGTTTCTCTTTTTCTTTGCGCCCCGTCTGGGGCAGCACTTCTGGTGGA‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAAAGGCTTTATC‑TTCGGGGGGGTTTACCACCAAAAAAACGG‑CTAACGCTTCTCGCGGGGGCCATGGGTGTTCTTCCACGGCC‑CCGGGGGCA‑TGGGGGGGGGGATACTGGTGAGACACCACAGTCCtgtgggggc                                                                        >  2:610052/1‑234 (MQ=255)

                                                                                                                                                                     tGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACggg                                                                      <  1:897040/238‑1 (MQ=255)

                                                                                                                                                                      gcgCCGTTTGTTTATACCAGCGGGGGTTCCTCTTTTGCTTCGCGCTCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGGTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGAt                                                                    <  1:1326622/237‑1 (MQ=255)

                                                                                                                                                                      gAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGAt                                                                    <  2:878815/239‑1 (MQ=255)

                                                                                                                                                                        gCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATc                                                                   <  2:188750/238‑1 (MQ=255)

                                                                                                                                                                              tgtttATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGAcaca                                                                                        <  1:1050374/211‑1 (MQ=255)

                                                                                                                                                                                          cggcggTTTCTCTTTTGCTTTGCGCCCAGGCTGGCGCAGCACTTCTTGGGGT‑TTCTCCTGGGGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGGTTTTCCACCAAAAAAACGT‑CTACCGTTTCTCGCGGCGGCTATTGTCGTTCTTTCA‑GCGC‑CCGCTGGGATTTGGGGGGGTAATACTGCTGAAGCACAACGGTCCTGTGCGCCGGGGTATGGACCATGTcgccccct                                                 >  2:1274617/1‑234 (MQ=255)

                                                                                                                                                                                          cggcggTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCCATGGTCGTTTTTTCC‑CCGC‑CCCTGGGGATTTGGGGGGGTAATACTTCTTGGAAACAACCGTTCTGTGCGGCCGGGTCttgtgccaaacgcccccg                                                 >  2:914684/1‑221 (MQ=255)

                                                                                                                                                                                             cggTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACa                                              <  1:336898/238‑1 (MQ=255)

                                                                                                                                                                                                    tctTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTc                                      <  2:1016105/239‑1 (MQ=255)

                                                                                                                                                                                                            ttCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                               <  1:576305/238‑1 (MQ=255)

                                                                                                                                                                                                            ttCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                               <  1:887209/238‑1 (MQ=255)

                                                                                                                                                                                                                cgcCCAGTCTGGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAATAAGCGT‑CTACCGTTTCTCGCGGCGGTTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGGGACACAACGATCCTGTGCGATGGGTTCTGGGTCACTTAGCCCCTTGCCACGGTTTCGACTTTCatat                           >  2:1131187/1‑238 (MQ=255)

                                                                                                                                                                                                                   ccAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcat                        <  2:243222/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcatcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:251166/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:1121117/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:1298924/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:414992/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:1132238/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:610052/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  2:199021/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  2:1079133/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  2:865534/238‑1 (MQ=255)

                                                                                                                                                                                                                               agcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAt            <  1:928292/238‑1 (MQ=255)

                                                                                                                                                                                                                               agcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAt            <  1:392448/238‑1 (MQ=255)

                                                                                                                                                                                                                                gcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAAAGGTTTCGACTTTCATATCATCGCCAATGCAATCt          >  2:1315955/1‑239 (MQ=255)

                                                                                                                                                                                                                                 caCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTa         >  2:389454/1‑239 (MQ=255)

                                                                                                                                                                                                                                  aCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTa         <  2:736458/238‑1 (MQ=255)

                                                                                                                                                                                                                                   cTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAtt       <  2:1286267/239‑1 (MQ=255)

                                                                                                                                                                                                                                     tCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGCCGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTgg     >  1:837714/1‑239 (MQ=255)

                                                                                                                                                                                                                                      cTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGTCCACGGTTTCGACTTTCATATCATCGACAATGAAAACTATTggg    >  2:430198/1‑239 (MQ=255)

                                                                                                                                                                                                                                        ggTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTTCTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt  <  1:252496/239‑1 (MQ=255)

                                                                                                                                                                                                                                        ggTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt  <  2:1267845/239‑1 (MQ=255)

CTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGT‑CTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCA‑GCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAT  >  NC_000913/4412657‑4413127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`