Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,536,214	G→A	16.3%	intergenic (+182/+401)	yjhR → / ← nanS	pseudogene, helicase family, putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,536,214	0	G	A	16.3%	164.1 / 3.9	71	intergenic (+182/+401)	yjhR/nanS	pseudogene, helicase family, putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase
Reads supporting (aligned to +/- strand):  ref base G (12/45);  new base A (10/2);  total (24/47)
Fisher's exact test for biased strand distribution p-value = 8.31e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAAAAATGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATAA  >  NC_000913/4535979‑4536448                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              gTAAAAAATGAAATAAAGTCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgca                                                                                                                                                                                                                                             <  1:26337/238‑1 (MQ=255)    aaaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gca                                                                                                                                                                                                                                        <  1:1069433/239‑1 (MQ=255)     aaaaTGAAATGAAGGCAATAAATGGGAGTCTGGAGCAACGGCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTGACCAATGTTAAGTCGACTTTCCGTTTTATTGCGAGTCACCTTTCCTGATTATAAATCTTACTGTTTTCCTAATCATTTTGCTTTACATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TGTTAGGACTG‑AAAGAAAATgcaa‑acaa                                                                                                                                                                                                                                       <  2:1274836/239‑1 (MQ=255)     aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:630128/239‑1 (MQ=255)     aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  2:469431/239‑1 (MQ=255)     aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:1146796/239‑1 (MQ=255)     aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:952703/239‑1 (MQ=255)      aaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTGACTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCaat                                                                                                                                                                                                                                      <  2:256208/239‑1 (MQ=255)          gAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAataata                                                                                                                                                                                                                                  <  2:639793/239‑1 (MQ=255)               aaaTGCAATATAGGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTTATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGTCTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAAtaatataata                                                                                                                                                                                                                             <  1:3960/239‑1 (MQ=255)                   gCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:1224707/225‑1 (MQ=255)                   gCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:1270000/225‑1 (MQ=255)                     aaTATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:252653/223‑1 (MQ=255)                       tatatGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgca                                                                                                                                                                                                                                             <  1:278039/216‑1 (MQ=255)                         tatGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:1252782/219‑1 (MQ=255)                         tatGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAAtaatataata                                                                                                                                                                                                                             <  1:377107/229‑1 (MQ=255)                            ggTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GT‑TTTTTTGTTTT‑GTAGCAAATATAT‑GCAAAAATATaataatcgggttatttt                                                                                                                                                                                                                >  2:466653/1‑228 (MQ=255)                             gTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGG‑TTTTTGGATTT‑AAAAAAAATG‑AA‑ACAAAAAAAaaataaaattttctttatt                                                                                                                                                                                                               >  2:1224707/1‑226 (MQ=255)                             gTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GG‑TTTTTGTTATT‑AAAGAAAAAGAAA‑GCAATAAAAtaataaatgtttcttttaa                                                                                                                                                                                                               >  2:1270000/1‑226 (MQ=255)                             gTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTTGTTATG‑TAAGAAAATGCAA‑ACAATAATATaataattgtctttttttt                                                                                                                                                                                                               >  1:537540/1‑227 (MQ=255)                               aGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGG‑GC‑GTTTTGGTCTG‑AAAGAAAATAGAA‑GCAAaaaaataaaaattgttttttatgacc                                                                                                                                                                                                             >  2:252653/1‑225 (MQ=255)                                 tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGTG‑GC‑TTTTTGGTATG‑TAAAAAAATACAA‑GAAATAATATAATAATAGCCTAATtttttttt                                                                                                                                                                                                           >  2:191298/1‑237 (MQ=255)                                 tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GG‑TTTTTTGTATT‑ACATAAAATGCAATCCATTAATAAAAAAATGCACTATTTTCttta                                                                                                                                                                                                            >  2:291815/1‑238 (MQ=255)                                 tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTTTTAATG‑AAAGAAAAAAGAA‑GCAataatatttgtctctttttcacatatgta                                                                                                                                                                                                           >  2:278039/1‑217 (MQ=255)                                  cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  2:64510/239‑1 (MQ=255)                                  cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  1:1284914/239‑1 (MQ=255)                                  cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  2:1030807/239‑1 (MQ=255)                                  cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  1:971343/239‑1 (MQ=255)                                  cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑a                                                                                                                                                                                                          <  2:740053/238‑1 (MQ=255)                                  cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCACTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  1:377101/239‑1 (MQ=255)                                  cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGGTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  2:79736/239‑1 (MQ=255)                                  cTGGATCAACGGCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTCTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  2:980212/239‑1 (MQ=255)                                   tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GT‑TTTTTTTTATT‑TAAGAAAAATCAA‑GCAATAAATTAATAAAGTGCTAATtttttttctt                                                                                                                                                                                                         >  2:377107/1‑235 (MQ=255)                                   tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GCGTTTTTGGATTG‑‑AAGAAAATACAA‑ACAAAAAAAtaaaaaatttttcttttatacata                                                                                                                                                                                                          >  2:1252782/1‑220 (MQ=255)                                   tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTTTTGTTGTAAAAAAAATG‑AA‑GAAATAATATAATAAATGACTAATttttttttaa                                                                                                                                                                                                         >  2:882913/1‑235 (MQ=255)                                   tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTTTGAATG‑AAAGAAAAAGCAA‑AACATAATATAAATATTGCCTAATtttttttata                                                                                                                                                                                                         >  2:377101/1‑235 (MQ=255)                                   tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑aa                                                                                                                                                                                                         <  2:1236499/238‑1 (MQ=255)                                         aaCGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCAtt                                                                                                                                                                                                  <  1:993584/239‑1 (MQ=255)                                          aCGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTa                                                                                                                                                                                                 <  1:291815/239‑1 (MQ=255)                                              cAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCa                                                                                                                                                                                             <  1:466653/239‑1 (MQ=255)                                                        tgtgATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATatcatcatc                                                                                                                                                                                   <  1:993977/239‑1 (MQ=255)                                                                    ttCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GT‑TTTTTTGTATG‑AAAAAAAAAAAAACACAAAAAAAAAAAAATAG‑CTTATTTTTTTG‑TAAATCATTAAACATATAATCATCGACTtttttttc                                                                                                                                                                       >  2:14928/1‑235 (MQ=255)                                                                            aCAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGt                                                                                                                                                               <  1:882913/239‑1 (MQ=255)                                                                                       aaaGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTtata                                                                                                                                                      <  1:720842/237‑1 (MQ=255)                                                                                        aaGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTTTGAATT‑AAAAAAAAAACAA‑ACAATAAAATAAAAATAGCCTAATTTTTTTT‑CACATAATTAAACATAAAATCATCGTCTATTTTCCAGAATCTCTCGACATTTAAt                                                                                                                                                    >  2:684727/1‑238 (MQ=255)                                                                                           ggCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTc                                                                                                                                                              <  1:397727/225‑1 (MQ=255)                                                                                           ggCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGG‑GG‑TTTTTTTGATG‑TAAGAAAAAACAA‑ACAATAATATTAAAATGGCCGTATTTTTTTT‑ATAATCATTTAAACTAACATCATATTCTATTTTCTATAATCAGTCGACTTTTAAtacc                                                                                                                                                 >  2:700906/1‑235 (MQ=255)                                                                                           ggCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGG‑GCTTTTTTGGATTG‑AAA‑AAAAAACAA‑GCAATAATATAAAAAAATGCATATTTTTTTT‑AACAACATTAATAAAATAATATTCATCAATTTTTCAGAAACCGTCGGCtttaatatcc                                                                                                                                                 >  2:987877/1‑230 (MQ=255)                                                                                             cATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GG‑TTTTTGGGATT‑GAAGAAAAAGTAA‑GGCATAATATAAAAAAGGCCCAATTTTTTTT‑TAAATCATTAAACATATAATCCTTTTCTATTTTCCACAATCCGTCCACTTATAAttaccca                                                                                                                                              >  2:565185/1‑233 (MQ=255)                                                                                             cATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGG‑GG‑TTTTTTGAATG‑AAAAAAAATGAAA‑GCAATAATATAATAATGGCCTAATTTTTTTT‑TAAAACATTAAGAATATCATCATCGTCTTTTTTTCAGAATCCGTCGACTTATaaaagacag                                                                                                                                              >  1:1088230/1‑232 (MQ=255)                                                                                              atatTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAg                                                                                                                                              <  1:684727/238‑1 (MQ=255)                                                                                                             tAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGa                                                                                                                               <  1:1314110/238‑1 (MQ=255)                                                                                                             tAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGa                                                                                                                               <  1:1209704/238‑1 (MQ=255)                                                                                                                    aCTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTTGGAATG‑AAAGAAAATGCTA‑GAAATAAAATAATAATAGGCGAATTTTTTTG‑GAAATCATTAAAAATATAATAAACGTCTATTTTTCAGAAACCGTCGACTTATTATTGCCAGAACACAATATCAGGACgtggcg                                                                                                                        >  1:1028264/1‑233 (MQ=255)                                                                                                                      tttCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCg                                                                                                                     <  2:1065766/239‑1 (MQ=255)                                                                                                                             tttATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCt                                                                                                              <  1:14928/238‑1 (MQ=255)                                                                                                                             tttATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCt                                                                                                              <  1:82167/238‑1 (MQ=255)                                                                                                                                         cGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACg                                                                                                 <  2:119632/239‑1 (MQ=255)                                                                                                                                         cGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACg                                                                                                 <  1:80727/239‑1 (MQ=255)                                                                                                                                         cGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACg                                                                                                 <  1:1285743/239‑1 (MQ=255)                                                                                                                                              ttCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCa                                                                                            <  1:472205/239‑1 (MQ=255)                                                                                                                                                   gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                       <  1:1075502/239‑1 (MQ=255)                                                                                                                                                   gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                       <  1:322089/239‑1 (MQ=255)                                                                                                                                                   gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                       <  1:565185/239‑1 (MQ=255)                                                                                                                                                      tataAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                       <  1:794419/236‑1 (MQ=255)                                                                                                                                                          aaTCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTa                                                                                <  1:538077/239‑1 (MQ=255)                                                                                                                                                            tCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gcaa                                                                                                                                                                                                                                       <  1:650997/88‑1 (MQ=255)                                                                                                                                                            tCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgca                                                                                                                                                                                                                                             <  1:722087/83‑1 (MQ=255)                                                                                                                                                             cTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATgcaa‑gc                                                                                                                                                                                                                                         <  1:620320/85‑1 (MQ=255)                                                                                                                                                               tATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGG‑GC‑TTTTTTGAATT‑AAAAAAAAATAAA‑GCAATAAAAAAATAAAGACCTTATTTTTTTT‑TACAACATTAAGCATATAAATATCCTCTTTTTTTCAGAATCCCTCGACATATAATTCCCACAACGGCCTATTAGGAAGGGGTCTCGAA‑CGCTGGTGCCCAAAAAGTTCCCGCAAACCGTGTAGgtgt                                                                           >  2:770888/1‑239 (MQ=255)                                                                                                                                                                aTTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑TG‑TTTTTGGGATT‑AAAGAAAAAGAAA‑GAAATAAAAATATAATGGGATAATTTTTTTG‑GAAAACATTAAAAAAATAATCATCGTCTATTTTCCAGAATACGACGAATTATTATCGCCAGGAAAGCATATTAGGAGGGGGCTGCGAACCGCT‑TGGCCCCAAAAGAACCAGGAAAAAGTTGAGATGt                                                                           >  1:197966/1‑238 (MQ=255)                                                                                                                                                                aTTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTTGGAATG‑AAAAAAAAAACAA‑GCAAAAATATAAAAAAAGCCTAATTTTTTTT‑AAAATCATTAAGCATATCATCATTGGCTATTTTCCAGAATCCGTCGACTTTTAATATCCAGAACGGCATATCTAGAAGGGGCTTCGAA‑CGCTGTGGCCCAAAACATTTCAGAAAACATTGTAGATGtt                                                                          >  1:1026374/1‑239 (MQ=255)                                                                                                                                                                                     cTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAAt                                                                                                                                                                   <  1:203622/130‑1 (MQ=255)                                                                                                                                                                                        tGCATAATGCGCCTGGGATTAATGGCGGGG‑GG‑TTTTTGGAATG‑GAAGAAAATGCAA‑GCAATAAAATAATAAAGGGCTAATTTTTTTT‑TAAATCATTAAAAAAAACATAATCGTCTTTTTTTCCAAATACGTCCACTTATAATCTCCCAAACCGCACATTAGGAAGGGGTTGCGGA‑AGCTGTGGCCCACAAACATCCACTAAAACTTGTAGGTGTTCGTAGGTTTCCGGGcccaaaacga                                                  >  2:604503/1‑231 (MQ=255)                                                                                                                                                                                           aTAATGCGCTGGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAAAAAATGCAA‑GCAAAAATATAAGAATGGCCTAATTGCTTTG‑AACATCATTAAGCATATAATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATAAGGACGGTGCGGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGaaa                                                <  1:210882/238‑1 (MQ=255)                                                                                                                                                                                               tgggcCAGGAATAAATGGCGGGT‑GC‑TTGTAGAAATG‑AAAAAAAATGAAA‑GCAAAAATATAATAATGGCTTAATTTCTTTA‑AAAAACATAAAACATATCATCATCGTCTATTTGCCAAAATCCGTCGACTTAAAATCGCCAGAACGAAATACAAGGAGGGTGCTGCAAA‑CGCTGTGGCCAAAAAGGTTCCAGTAAACTGTGTAGAGGTTCGTAGATTTCCGAGCCATAATCGAAAAGgcg                                           <  2:1183074/236‑1 (MQ=255)                                                                                                                                                                                                    cTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGAt                                      <  1:277243/239‑1 (MQ=255)                                                                                                                                                                                                     tGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAACCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTCCCGAGCCATAATCGAAAAGGCGCGGATg                                     <  1:700906/239‑1 (MQ=255)                                                                                                                                                                                                     ggggATAAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAAAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATg                                     <  1:987877/238‑1 (MQ=255)                                                                                                                                                                                                        gATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                       <  1:710665/186‑1 (MQ=255)                                                                                                                                                                                                                  gggT‑GC‑TTTGAGGAATA‑AAAGAAAATACAA‑GAAAAAATATAAAAATGGCCTAATTTCCTGG‑AATATAATTAAGCATAGCATCACCGTCATTTTGCCAGAACCCGTCGACTTATAAGCGCGAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCGATAAAACTGTGTGGATGTTCGTGGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCa                         <  2:1203606/238‑1 (MQ=255)                                                                                                                                                                                                                        c‑TTTTTGGAATG‑AAAGAAAAAGCAA‑ACAAAAATATAAAAAAGGCCTAATTTTTTTT‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCCTCGACTTATAATAGCCAGAACGGCATATCAGGACGGTGCTGCGGA‑CGCTGTGGCCCAAAACGTTCCAGTAAAATGTGTAGATGTTTGTAGATTTCCGAGCCATAATCCAAAAGGCGCGGGTGTCTATTAAGGCATcccg                    >  2:708034/1‑236 (MQ=255)                                                                                                                                                                                                                          tttaAGGAATG‑AAAAAAAATACAA‑ACATAAATAGAATAA‑GGGCTAATTTTTTTGTAACACCTTAAAGCAATACATCACGTTCGTTTTACCATAATTCGTCTATTTATAAGCGCAAAAACGGCATACCAGGTCGGGGCTGCAAA‑CGCTGTGCCCCAAAACTTTCAGGTAAACTGTGTAGATGTCGGTAGATTCCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCt                  <  2:7096/239‑1 (MQ=255)                                                                                                                                                                                                                                           aaaTGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAGCGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAAt    <  1:764276/237‑1 (MQ=255)                                                                                                                                                                                                                                           aaaTGCAA‑GCAATAAAATAATATGGGCCTATTTTCTTTG‑ACCATCATTAAGCATATCATCATCGTTTATTTGCAAGATCCCGTCGACTTATAATCGCAAGAACGGCATACCAGGCCGGTGCTACGAG‑CGCTGGGGCACAAACCGTGCCATAAAACTGTGTATATGTTCTTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATaa  <  2:197966/239‑1 (MQ=255)                                                                                                                                                                                                                                           aaaTGCAA‑GAAATAATATAATAATGGCCTAATTTCTTTA‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAAt    <  1:124493/237‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              GTAAAAAATGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGT‑GC‑TTTTAGGAATG‑AAAGAAAATGCAA‑GCAATAATATAATAATGGCCTAATTTCTTTG‑AACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAA‑CGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATAA  >  NC_000913/4535979‑4536448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑