BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	65,670	T→G	16.1%	A37A (GCA→GCC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,670	0	T	G	16.1%	307.6 / 7.6	119	A37A (GCA→GCC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand): ref base T (20/75); new base G (21/3); total (41/78)
Fisher's exact test for biased strand distribution p-value = 2.44e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCA  >  NC_000913/65433‑65907

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                         <  2:1325237/239‑1 (MQ=255)

cATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTg                                                                                                                                                                                                                                                         <  2:704172/239‑1 (MQ=255)

       tAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGc                                                                                                                                                                                                                                                    <  2:31672/237‑1 (MQ=255)

       tAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCg                                                                                                                                                                                                                                                   <  2:817537/238‑1 (MQ=255)

          cGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGGGCAAGCGTaa                                                                                                                                                                                                                                                >  1:282793/1‑238 (MQ=255)

           gTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑c                                                                                                                                                                                                                                              <  1:208696/238‑1 (MQ=255)

            ttCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGGAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGGACCTGATCGGCGGGGAAAAACG‑CCACCGACTCTTGCGGGGGCAGCGCAA‑ccg                                                                                                                                                                                                                                            >  2:67528/1‑238 (MQ=255)

                 gCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAAAGCCTTGTTTACCCTGCAAAATATTCTGAGCGCGGGGAACCTGATCGGCGGGAGACACCC‑CCACCGACCGTTGCGCGGCAAGCCGAA‑CCTG‑CCAc                                                                                                                                                                                                                                      >  2:1072640/1‑239 (MQ=255)

                    gACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGAAAAAAG‑CCCCGGAGCCCTGCGGGGGAAGCGTAA‑ACCG‑CAACgg                                                                                                                                                                                                                                    >  2:365581/1‑238 (MQ=255)

                    gACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCCTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAAAC‑ACACCGACTCTTTCGGTGCAAggcacaccctccaccg                                                                                                                                                                                                                                      >  2:926760/1‑223 (MQ=255)

                      cGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGAAAAAAG‑CCCCCGGCCCTTGCGGGGGAAGGGGAA‑CCTG‑CCACgggc                                                                                                                                                                                                                                  >  2:277906/1‑236 (MQ=255)

                      cGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCACCGGTGTCAGGCGCAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAAAAAACC‑CCACGCACACCTGGCGGGCAAGCgcacacctcaccggca                                                                                                                                                                                                                                    >  2:1009158/1‑223 (MQ=255)

                                  tCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCgc                                                                                                                                                                                                                     <  2:440395/239‑1 (MQ=255)

                                     tAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAAG‑CCACGGACTCTTGCGGGGCAAGGGTAA‑ACTG‑CAACGGGCCGTTTTCCgccgccc                                                                                                                                                                                                                   >  1:515309/1‑237 (MQ=255)

                                          ggTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTTAGCGCGGGGAACCTGATCGGCGGGGATAAACG‑CCCCGGGCTCTTGCGGTGCCAGCGTAA‑CCCG‑ACACGGccccgtttcccgcgcccccccca                                                                                                                                                                                                             >  2:1133296/1‑219 (MQ=255)

                                          ggTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAAAAAACG‑CCCCGGACCCTTTCGGGGCAAGCGCAA‑CCCGCCAACGGCCC‑TTGTCCGTCgcccccccga                                                                                                                                                                                                             >  2:911048/1‑232 (MQ=255)

                                              aCGCCCCCTTCACGCAGGCGCTTTGCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGAGGTTAAAAGCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                        <  2:1277717/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                        <  2:1242034/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                        <  1:487175/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                        <  2:1030138/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                        <  2:1013099/239‑1 (MQ=255)

                                              aCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                        <  2:777251/239‑1 (MQ=255)

                                              aCGCCACCTTCACACAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGga                                                                                                                                                                                                        <  1:255337/239‑1 (MQ=255)

                                                     cTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCt                                                                                                                                                                                                 <  1:41114/239‑1 (MQ=255)

                                                       tCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGt                                                                                                                                                                                               <  1:326594/239‑1 (MQ=255)

                                                       tCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGt                                                                                                                                                                                               <  2:130046/239‑1 (MQ=255)

                                                        cACGCAGGCGCTTTTCGTAATTCATCAATGGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGt                                                                                                                                                                                               <  1:1190819/238‑1 (MQ=255)

                                                               gcgcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAAAG‑CCACGGACCCTTTCGGGGGCAGCGTAA‑CCCG‑CCACGGCCCCGTGTCCGTCGCCAC‑CCAGAAAGAGACATCTGT‑CCCCTGCg                                                                                                                                                                                      >  2:26235/1‑239 (MQ=255)

                                                                 gcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CTACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGATGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCgg                                                                                                                                                                                     <  1:37665/238‑1 (MQ=255)

                                                                   ttttCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGgt                                                                                                                                                                                  <  1:365581/239‑1 (MQ=255)

                                                                          aaTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCg                                                                                                                                                                            <  1:299837/238‑1 (MQ=255)

                                                                          aaTTCATCAAGTGGCGAGGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTAAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCCCCCTGAAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGAAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGCCTTCTGT‑CCCTTGCGGGGTGTCCCGc                                                                                                                                                                           <  2:1016649/239‑1 (MQ=255)

                                                                                tcaATTGGCGATGGGCGCGACAATAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAgtgt                                                                                                                                                                     <  1:863883/239‑1 (MQ=255)

                                                                                    ttGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACA‑CCACCGGCTCTTTCGGGGGAAAAGCAACCCCC‑CAAGGGCCCGTT‑TCCGTCCCCCG‑CCCGAACGGGACTTCTGT‑CCCCTGCGGGGGGTCCCGCCCGTGTC‑ggg                                                                                                                                                                >  2:579222/1‑239 (MQ=255)

                                                                                       gCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAAG‑CCCCCGGCTCTTGCGGGGGAAACGTAA‑ACCG‑CCACCGCCCCCTGTCCCTCCCCCGCCCAGAGGGGGAC‑TCTTT‑CCCCTTCGGGGGGGCCCCCCCGGGGC‑ggggtg                                                                                                                                                             >  2:1108408/1‑236 (MQ=255)

                                                                                                            gCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCCCGGACTCTTTCGGGGGAAGCGTAA‑ACTG‑CCACGGCCCCTTTTCCGTCGCCCA‑CCCGAAGGGGACTTCTTT‑CCCCTGCGGGGTGTCCCCCCCGTGGC‑GGGTTTAGA‑TAAAACCTGCCTCCGcc                                                                                                                                        >  2:591459/1‑238 (MQ=255)

                                                                                                             ccATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCa                                                                                                                                       <  1:322698/238‑1 (MQ=255)

                                                                                                                 acacCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACCCTTTCGGTGGCAGCGTAA‑ACTG‑CAACGGCCCGTTTTCCGGCGCCAA‑CCCCAAGGAGACTTCTGT‑TCCTTTCGGGGGGGTCCCCCGGTGGC‑GGGGTTAGAGTAAAActccccccgccccccc                                                                                                                                    >  2:1008293/1‑223 (MQ=255)

                                                                                                                 acacCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAAGACCCCGGA‑TCTTGTGGGGCAAAGGTAA‑CCTG‑CAACGGGCCGTTGTCCCGCGCCCG‑ACAGAAGGAGACTTCTGTCCCCTCGCGGGG‑GTGCCGCCCGGGTC‑GGGGTTAGA‑AAAAAacagccccgcccccgccc                                                                                                                                  >  2:930373/1‑227 (MQ=255)

                                                                                                                            cGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATc                                                                                                                        <  1:370207/238‑1 (MQ=255)

                                                                                                                               tGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAAG‑CCCCGGGCTCTTGCGGGGCCAGCGTAA‑ACTG‑CAACGGCCCGTTGTCCCTCGCCCG‑CCCGAAGGAGACTTCTTT‑CCCTTGCGGGGGGGCCCCCCCCGGGC‑GGGGTTAGG‑TAAAACCCGCCCGcccccccgcggaaatccctccaaa                                                                                                                    >  2:597539/1‑219 (MQ=255)

                                                                                                                                  aaaTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGCCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑c                                                                                                                <  1:67528/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCGGGGGGGTAAGGCGAAAGCCTTGTTCACCCGGCAAAATATGCTGGGCGCGGGGAACCTGGACGGCGGGAAAAAACG‑CCACGGTCTCTTGCGGGGGGAGGGTAA‑CCTG‑GAGCGGCCCGTTGTCCGTCGCCGG‑CCAAAAGGGGGCTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  2:345737/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  2:481280/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  1:63993/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  1:1108408/239‑1 (MQ=255)

                                                                                                                                    aTCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCa                                                                                                              <  1:833218/239‑1 (MQ=255)

                                                                                                                                       cTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGc                                                                                                          <  1:374158/239‑1 (MQ=255)

                                                                                                                                        tttAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCt                                                                                                         <  1:179813/239‑1 (MQ=255)

                                                                                                                                        ttgaaCGCCAGCGGTGTCAGGCGAAGGCCGTGTCCACCCTGCAAACGGTGCTGAGGGGGGGGAACCTGATCGTCGGCACTAAACG‑CCGCGGACTCTTGCGGGGCAACCGTAC‑CTTG‑CACCGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGCGCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTG‑AAATCCATCAAAAAA‑CCA‑GGCtt                                                                                                        <  2:450340/236‑1 (MQ=255)

                                                                                                                                             cGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGt                                                                                                    <  1:1018365/239‑1 (MQ=255)

                                                                                                                                              gCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGta                                                                                                   <  1:788263/239‑1 (MQ=255)

                                                                                                                                                        tCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGt                                                                                       <  1:911048/238‑1 (MQ=255)

                                                                                                                                                             cGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAAAC‑CCCCGGACTCTTGCGGTGCAAGCGTAA‑ACTG‑CAACGGCCCGGTGTCCGTCGCCAG‑CCCGAAGGAGGCTTCTGT‑CCCCTTCGGGGGGGCCCGCCCGGGGCGGGGTTAAAA‑AAAAACC‑GCCTGCGCCCCCCGGGAAAACCATCAAAAAA‑ACA‑GGGTTGAGAATATG‑CCG‑GGtttgtt                                                                                  >  2:224483/1‑236 (MQ=255)

                                                                                                                                                               aaaGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGGCTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAC‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGGGTCCCCCCAGTGGCGGGGTTAAG‑‑GAAAAACTGCCCTCGCCCCGCTGAAAAACCATCAAAAAACCCA‑GCCTTG‑GTAAA‑GCCCT‑GGttt‑gtttg                                                                               >  2:1188080/1‑239 (MQ=255)

                                                                                                                                                                  gCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGGGTCCCGCCCGTGGC‑GGGGTTAGA‑TAAAAACCTCCCGCGCCACGCTGGAAAACCCACCAAAAA‑CCA‑GGCGTTGGGATT‑A‑CCC‑GGTTTTgtttgttt                                                                            >  2:147273/1‑239 (MQ=255)

                                                                                                                                                                       gTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  1:277906/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCTGCAAAATAGGCTGAGCGGGGGGAACCTGATCGGCGGGAATAAACG‑CCAGGGACTCTTGCGGTGAAAGCGTAA‑CCTG‑AAACGGCCCGTTGTCGGTCGCCAG‑CCAGAAGGAGACTACTGT‑CCCTTGCGGGGTGTCCCGGCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  1:977219/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCGGCAAAATATGCCGAGCGGGGGGAGCCTGATCGGGGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAC‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  2:515309/239‑1 (MQ=255)

                                                                                                                                                                       gTTCACCCGGCAAAAAATGCTGAGCGGGGGGAACCTGATCGGCGGGAATAAACG‑CCCCGGACTCTTGCGGTGCAAGCGTAC‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGC‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCtg                                                                       <  2:518784/239‑1 (MQ=255)

                                                                                                                                                                             ccTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTTCGGGGTGTCCCGCCAGTGTC‑GGGGTAAGA‑TAAAAACTGTCCGCGCCACGCTGAAAATCCATCCAAAAA‑CCC‑GGCTTCGGGATA‑A‑CCT‑GGTTTTGTTTTTTTGGCTGGGGgtt                                                                 >  2:653722/1‑239 (MQ=255)

                                                                                                                                                                             ccTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGGGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGGGTCCCGCCAGTGTC‑GGGGTAAAA‑AAAAACCTGCCCGCGCCCCGCTGGAAATCCATCAAAAAA‑AAA‑AGGTTGAGGATA‑G‑CCC‑GGGTTCTTTTTAtttggtttgggtt                                                                 >  2:275577/1‑228 (MQ=255)

                                                                                                                                                                              cTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑TCCCTGCGGGGGGTCCCGCCCGTGTC‑GGGTTAAAA‑TAAAACCCGCCTGCGCCCCGCTGGAAATCCCTCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTTGTTGGGCTTGGtttt                                                                >  2:1057315/1‑239 (MQ=255)

                                                                                                                                                                               ggCAAAATATGCTGAGCGCGGGGACCCTGATCGGCGGGAATAACCG‑CCACGGCCTCTTGCGGTGCAAGCGTAC‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTta                                                               <  2:1156825/238‑1 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGGGGGGGGGGAACCTGATCGGCGGGAATAACCT‑CCGCGGACTCTGGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTGGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAAGCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  2:395509/239‑1 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  1:1225153/239‑1 (MQ=255)

                                                                                                                                                                                gCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTtat                                                              <  1:926760/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaTATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCCCGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:1009158/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaTATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:1167058/239‑1 (MQ=255)

                                                                                                                                                                                  aaaaGATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  2:282793/239‑1 (MQ=255)

                                                                                                                                                                                     atatGCTGAGCGCGGGGAGCCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTGGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGt                                                         <  1:1210888/239‑1 (MQ=255)

                                                                                                                                                                                       atGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGGTAAGA‑TAAAAACTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑ACA‑GGCTTGAGGATT‑G‑CCC‑GGTTTCGTTTGATTGGGTGTGGTTTTTTACAGTCa                                                       >  1:28056/1‑239 (MQ=255)

                                                                                                                                                                                        tGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                            <  1:1069545/233‑1 (MQ=255)

                                                                                                                                                                                         gCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CCACGGCCCGTTGTCCGTCGCCCG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGGGTCCCGCCAGTGGC‑GGGGTAAAA‑TAAAAACTGCCCGCGCCCCGCTGAAAAACCATCAAAAAA‑ACA‑CGGTTGAGGATA‑A‑CCT‑CGTTTTGTTTGTTTGGGGGGGGTTTTTTACAgcactt                                                     >  2:919037/1‑234 (MQ=255)

                                                                                                                                                                                           tGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTTCGGTGCAAGCGTAA‑CCCC‑CAACGGCCCGTTGTCCGTCCCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGGGGCCCCCCCGTGTC‑CGGGTTATA‑TCAAACCTCCCCTCGCCCCCCTGAAAATCCATCAAAAAA‑CCC‑CGCCTGAGGGTT‑G‑CCTCGGTTCGGTTTATTTGTCAGTGTTTTTTTACATTcttat                                                    >  2:20053/1‑235 (MQ=255)

                                                                                                                                                                                           tGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTa                                                    <  1:597539/238‑1 (MQ=255)

                                                                                                                                                                                            gAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                  <  1:1135480/239‑1 (MQ=255)

                                                                                                                                                                                              gcgcGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGGGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGGGTCCCGCCCGTGTC‑GGGGTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCCTCAAAAAA‑ACA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGGTGTGGGTTTTTAAAGTCAtttatt                                                 >  2:550714/1‑234 (MQ=255)

                                                                                                                                                                                                gcgGGGAACCTGTCCGGAGGAAATAAACG‑CCACGGACTCTTGCGGTGCAAGCTTAC‑CCTG‑CAACGGCCCGTGGTCCGTCGCCAG‑CCAGAATGAGACTTCTTT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  2:378/238‑1 (MQ=255)

                                                                                                                                                                                                gcgGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  1:387394/238‑1 (MQ=255)

                                                                                                                                                                                                  ggggAACCTGATCGGCGGGAATAAACG‑CCACGGGCTCTTTCGGGGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCCGAAGGAGACCTCTGT‑CCCTTGCGGGGGGTTCCGCCAGTGGC‑GGGGTAAGA‑TAAAACCTGCCCTCCCCCCGCCGAAAAATCACCCAAAAA‑CCAGGGTTTGGTTAAT‑G‑CCG‑GGTTTGGTTTTATTGGTGGGGGTTTTTTACATGCATTCCtcttgt                                             >  2:1069545/1‑234 (MQ=255)

                                                                                                                                                                                                  ggggAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcc                                               <  1:535448/236‑1 (MQ=255)

                                                                                                                                                                                                         cTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCt                                                                                                         <  1:417810/174‑1 (MQ=255)

                                                                                                                                                                                                           gATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGGGCAAGCGTAA‑CCTG‑CACCGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCt                                   <  2:452020/239‑1 (MQ=255)

                                                                                                                                                                                                                  gggAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCgcgc                             <  1:1072640/238‑1 (MQ=255)

                                                                                                                                                                                                                  gggAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTGGCCCGCCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCgcgc                             <  2:363327/238‑1 (MQ=255)

                                                                                                                                                                                                                       tAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTGGTCCGTCGCCAG‑CAAGAAGGAGACTTCTGC‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCt                       <  1:926031/239‑1 (MQ=255)

                                                                                                                                                                                                                                aCGGACTCTTGCGGTGCAAGCGTAG‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               <  1:662599/239‑1 (MQ=255)

                                                                                                                                                                                                                                aCGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATTCCTTCCCGCCCTGCTTACGCAgt               >  2:174171/1‑238 (MQ=255)

                                                                                                                                                                                                                                aCGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGa               <  1:174171/239‑1 (MQ=255)

                                                                                                                                                                                                                                 cGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCGAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt              <  1:711437/239‑1 (MQ=255)

                                                                                                                                                                                                                                 cGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt              <  1:550714/239‑1 (MQ=255)

                                                                                                                                                                                                                                    aCTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgt           >  1:611669/1‑239 (MQ=255)

                                                                                                                                                                                                                                     ctctTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgt           <  1:39819/238‑1 (MQ=255)

                                                                                                                                                                                                                                      tctcGCGGTACAAGCTTAA‑CCTG‑AAACGACCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGGGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtgt         <  1:563627/239‑1 (MQ=255)

                                                                                                                                                                                                                                      tctTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtgt         <  1:1057315/239‑1 (MQ=255)

                                                                                                                                                                                                                                      tctTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgtgt         <  1:224483/239‑1 (MQ=255)

                                                                                                                                                                                                                                        ttGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                  >  2:612718/1‑196 (MQ=255)

                                                                                                                                                                                                                                         tGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTa      >  2:575654/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCca  >  1:713663/1‑239 (MQ=255)

CATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACG‑CCACGGACTCTTGCGGTGCAAGCGTAA‑CCTG‑CAACGGCCCGTTGTCCGTCGCCAG‑CCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTC‑GGGTTAAGA‑TAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAA‑CCA‑GGCTTGAGTATA‑G‑CCT‑GGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCA  >  NC_000913/65433‑65907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`