Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,151,681	G→T	23.0%	intergenic (+35/+30)	yegJ → / ← yegK	uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,681	0	G	T	23.0%	31.1 / 4.3	38	intergenic (+35/+30)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (14/10);  new base T (4/4);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATA  >  NC_000913/2151443‑2151912                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTg                                                                                                                                                                                                                                                <  1:561242/239‑1 (MQ=255)      taatGGCGTAATCAGCAATGAACCTGGTATTGTTAAAAACGTAGAATATGGCTAGTATTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACGATTGATCCTTTATTGGTCGCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑ttg                                                                                                                                                                                                                                         <  2:947592/236‑1 (MQ=255)      tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑ttg                                                                                                                                                                                                                                         <  1:396693/239‑1 (MQ=255)              tAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATTCTTTTTTTGTTACCTTTCCAAAAGAAGAAAACGACGAGCGAAGAGCAAAAATAGTTTCCTTATCAAACGTTATATTTTTTTTTTAAAATAGCGG‑TGT‑AA‑TTTTTG‑ttttt                                                                                                                                                                                                                                >  1:699365/1‑238 (MQ=255)                                                         gcAGGGGGGGAAAAAAAAAAAAAAAGAATATTTAAGGTTGGATGTATACGCGGGGGGAAAAAATTTCGGGGGTTTATACCATTGATCTTTTTTTGGTCCCCTATCAAAAAGAAAACGCCGAAGAGAAAAGAGAAAAATTAGTGCGTTAATCAGCCTTTTTTTTTTTTTTTAAAACGGGGGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGc‑c                                                                                                                                                                                    <  2:468798/237‑1 (MQ=255)                                                              aGTAGAAAAACAAAAAAGAAT‑ATAGTTAAGACTGGTGTTATAGGCGGGGGAATAAATTTCGCGGGTGTTATACATTTAATCCTTTATGGCCCTCCAACCAAAAAGAAAAAGCGGATGAGCTAAGAAAAAAATTAGTTCGATAGCCAGCGTTTATATTTTTTGTTTAACCGGGGGG‑TTG‑AA‑TTGTTT‑TCTC‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGtt                                                                                                                                                                              <  2:972473/238‑1 (MQ=255)                                                                     aaTAAAAAAAAAAG‑ATATTTCAGCCTGGATGTATCCGCGGGGGAAAAAAATTTACGGCGGTTATCCCATTGTTCTTTTATTGCTCACCTACCAAAAAGAAGAAGCCAACGAGAAAAGAAAAAAATATGTTCGTTAATCACCCTTTATATTTATTGTTTAACCTGGGGG‑TTA‑AA‑TTTTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTatcatc                                                                                                                                                                      <  1:443884/239‑1 (MQ=255)                                                                                     tacctcatgcTGGAGTTACACGCGCTGGAATAAAATTTACGGGCTTTACACTTTGGACCCTTTATGGCACACCTAGCAAAAAAAAGGAGGCGACAGGTCAAGAAAAAAAAGTCTGCGCTACACACCCGTTTTATTTATTGTTTAGCCTGGGGG‑TAA‑AT‑TTGTT‑‑TCCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGtt                                                                                                                                                      <  2:115863/230‑1 (MQ=255)                                                                                                                                 gTTATACCATTGATCCTTTATTGGTCCCCTACCAAAAAGAAAAAGCCGAGGAGCTAAGAGAAAAATTAGTCCGCTAACCAGCCGTTATATTTATTGTTTAAACGGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTatcatcat                                                                                                                                                                    <  1:211309/182‑1 (MQ=255)                                                                                                                                        ctttGATCTTTTATTGGCCCCCTACCAAAAAGAAAAAGCCGAAGAGCTAAGAGAAAAATTAGTTCGTTAATCAGCTTTTATTTTTTTTTTTAAAACGGGGGT‑TTA‑AT‑TTTTTT‑TCTC‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                   <  1:544188/237‑1 (MQ=255)                                                                                                                                           tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑TA‑TTTTTT‑TTTT‑CCA‑CCGCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGC‑CAACGTTTTATCCTCCT‑CCGTACCCTc                                                                                                                                                         >  2:211309/1‑182 (MQ=255)                                                                                                                                            gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CCA‑CCGCCATGAGGAAATCAATCTGCCCCCAGCGCCAGTGC‑CAACCGTTTATCATTAT‑CCGTTAGCCCGTGTAACGCCGGGCTGGACCAAAACTGCTTTTACAATTTCGGTTATAAATCGAgttgt                                                                                               >  1:430787/1‑239 (MQ=255)                                                                                                                                                  ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGC‑CAACGGTTTTTCATCAT‑CCGTAAGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAAACGAGTTGTTCCTGc                                                                                         >  1:567766/1‑239 (MQ=255)                                                                                                                                                   tATTGCTCCCCTACCAAAAAAAAAAAGCCGACGAGCTAAGAGCAAAATTATTCCGTAAATCAGCTGTTATATTTATTTTTAAAACTGGGGG‑TTA‑AA‑TTGTTG‑TCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg                                                                                        <  1:914921/239‑1 (MQ=255)                                                                                                                                                        gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTTTTG‑TCTT‑CAA‑CCCCCATGAGAAACTCACTCTGCCCCCAACGCCCGTGC‑CCACGGTTTTTCATCAT‑CCGTACGCTCGTTTACCGCCCGGCTGGACAAAAACATCTTTAACAATTTAGGGAATAAATCGAGTTGTTCCTGCGTtgct                                                                                   >  2:268980/1‑239 (MQ=255)                                                                                                                                                         tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑TA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGC‑CCACGGTTTATCATCAT‑CCGTACACTCGTTTTCCGCCCGGCCGGACAAAAACTGCTTTAACCAATCCGGGAATAAATCGAGTTGTTTCttggtttct                                                                                   >  2:977994/1‑230 (MQ=255)                                                                                                                                                         tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTTCTGCGTtgct                                                                                   >  1:897092/1‑238 (MQ=255)                                                                                                                                                          cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTT‑GA‑TTGTTG‑TGCT‑CCA‑CCGCCCTGGGAAAATTACTTCCCCCCCAACCCCCGTGCGAAACCTTTTTTCA‑CAT‑CCGGACGCCCGTTTTCCACCCGGCTCGACCCAAACTGCTTTTACCATTTAGGTaaaaaaattctgtgtttcttcgtcgctcg                                                                                 >  2:1147034/1‑216 (MQ=255)                                                                                                                                                           aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCCCGTTTACCCCCCGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAACGAGTTTTTTCTGCggttcttc                                                                                 >  1:1091478/1‑231 (MQ=255)                                                                                                                                                                                        aaGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TGTT‑CAA‑CCCCCATTAGAAAATCACTCTGCCCCCAGCGCCAGTGC‑CAACGGTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGGTGGGCAAAAACTGCTTTCACAATTTCGGCAATAAATCGAGTTTTTCCTGCGGTTCCGCCGCCAGTCCCATGGAAAACGGGGGaaagca                                                   >  2:749916/1‑237 (MQ=255)                                                                                                                                                                                              aaaaTTTGTTCCATAAACAGCCCTTATTATTTTTTTTTAAACCGGGGG‑TTT‑AATTTTTTG‑TTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGca                                              <  1:295498/239‑1 (MQ=255)                                                                                                                                                                                              aaaaTTGGTTCCTTAATAACCCTTTATTTTTTTTGTTAAACCTGG‑GG‑TTT‑AA‑ATTTTT‑TCCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                             <  1:500990/239‑1 (MQ=255)                                                                                                                                                                                                aattgtttcgttaaaccccctttATTTTTTTTG‑TTAAACTGGGGG‑TTT‑AA‑ATTTTGTTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACAt                                           <  1:1030252/218‑1 (MQ=255)                                                                                                                                                                                                   tAGTTCGTTATACACCCTTTTTTTTTTTTATTAAACCTGG‑GG‑TTT‑AA‑TTTTTT‑TTTTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                        <  1:99038/239‑1 (MQ=255)                                                                                                                                                                                                     tgtccatAATCAGCCGTTATTTTTATTGTTAAAACTGGGGG‑TTG‑AA‑TTTTTG‑TCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagga                                      <  1:307402/233‑1 (MQ=255)                                                                                                                                                                                                          cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑ATG‑TA‑TTGTTT‑TTTT‑TCA‑ACGCCATGAGAAAATAACTCTGCCCACAACCCCCATGC‑CCACGTTTTTTTATCATCCCGTAC‑CTCTGTTACCGCCGGGGTGGACAAAAAATTCTTTTACAATTCATGGAATAAATAGCGGTGTTTCTGCGGTTCTTCCGCCAGTCCATTGTAAAACGGGGAAAAAAAAGGCACATGAGGGGAAtt                                 >  2:1231099/1‑239 (MQ=255)                                                                                                                                                                                                             ccagctcttaaaatttgttTTATAAAGTGGCGT‑TTG‑AT‑TTTTTG‑TCTA‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                              <  1:757242/227‑1 (MQ=255)                                                                                                                                                                                                             acaaGCCTTTTTATTTATTGTTAAACCGGGCGG‑TTG‑AT‑TTTTTT‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                              <  1:872061/236‑1 (MQ=255)                                                                                                                                                                                                                  ccGTTATATTTATTGTTTAAACTGGCGG‑TTG‑GA‑TTGATG‑TCTT‑CCG‑ACGCCATCAGAAAATCACTCTTCCCCCAACGCCAGGGC‑CAACCTTTTATCATCAT‑CCGAAAGCTCGTTTTTCCCCGGGCTGCACCAAAAATGCTTTAACAATTCCAGCAATAATTCATGTTGTTCCGGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGATAAACAAATGCACATGAGGAGAAGTATCCAGCa                         >  2:636701/1‑239 (MQ=255)                                                                                                                                                                                                                             attGTTTAAACTGGCGG‑TTG‑AA‑TTGTTT‑TCTT‑CAA‑CCGCCTTGAGAAAATCACTCTGACCACAGCGCCAGTGC‑CAACGTGTTTTCATCAT‑CCGTTCGCTCGTTTAACGCCGCGCTGGACAAAAAATTCTTTTACAATTCAGGTAATAAATTGAGTTGTTTCTGCGTTGCTGCCGCCAATCCATTGGAAAAAGGGGAGAAAAAAGGGACAATAGGAGAATTATCCAGGATATTTAACGcc              >  1:1331392/1‑239 (MQ=255)                                                                                                                                                                                                                              attattAAACCGGCGGTTTG‑AC‑TGTTTG‑TCAC‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc              <  1:1231099/235‑1 (MQ=255)                                                                                                                                                                                                                               tGTTTAAACTGGCGG‑TTT‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGGTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg            >  1:753409/1‑239 (MQ=255)                                                                                                                                                                                                                               tGTTTAAACTGGCGG‑TTT‑AA‑TTGTTG‑TCTT‑CAA‑ACCCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCGGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTCCCTTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg            >  1:760198/1‑239 (MQ=255)                                                                                                                                                                                                                               tGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TTTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCCCAACGCCAGTGC‑CCACGTTTTATCATCAT‑CCGTACGCCCGTTTACCGCCGGGCTGGACAAAAAATGCTTTTACAATTCAGGTAATAAACTGAGTTGTTCCTGCGGTGCTGCCGCCCGTCCATTTGAAAACGGGGGAAAGAAAAGCACAAGAGGAGGATTATCCAGCCTATTTAACGcccg            >  1:66511/1‑237 (MQ=255)                                                                                                                                                                                                                               tGTTTAAACTGGCGG‑CTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACCCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCCCGTTTTCCGCCGGGCTGGACAAAAACTTCTTTAACAATTCCGGTAATAAATCGAGTTGTTCCTGCGGTTCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTTACGCCaa            >  1:884981/1‑238 (MQ=255)                                                                                                                                                                                                                               tGTTAAAACGGCCGT‑TTA‑AT‑TTTTTG‑CCTA‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg            <  1:1095048/239‑1 (MQ=255)                                                                                                                                                                                                                                 tttAACCGGGGGG‑TTA‑AA‑TTGTTG‑TCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                   <  1:1315240/150‑1 (MQ=255)                                                                                                                                                                                                                                           ggg‑TTGAAA‑TTGTTT‑TTTC‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa  <  1:1012115/237‑1 (MQ=255)                                                                                                                                                                                                                                           cGG‑TTG‑AA‑TTTTTT‑TCTT‑TAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGGTTTATTATCAT‑CCGTACGCCCGTTTTCCCCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGGAATAAATCGAGTTGTTTCTGc                                                                                         >  2:1315240/1‑150 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATA  >  NC_000913/2151443‑2151912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑