Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,151,689	G→T	27.3%	intergenic (+43/+22)	yegJ → / ← yegK	uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,689	0	G	T	27.3%	29.7 / 2.6	36	intergenic (+43/+22)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (14/11);  new base T (4/6);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAC  >  NC_000913/2151456‑2151913                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      tAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATTCTTTTTTTGTTACCTTTCCAAAAGAAGAAAACGACGAGCGAAGAGCAAAAATAGTTTCCTTATCAAACGTTATATTTTTTTTTTAAAATAGCGG‑TGT‑AA‑TTTTTG‑ttttt                                                                                                                                                                                                                                 >  1:699365/1‑238 (MQ=255)                                            gcAGGGGGGGAAAAAAAAAAAAAAAGAATATTTAAGGTTGGATGTATACGCGGGGGGAAAAAATTTCGGGGGTTTATACCATTGATCTTTTTTTGGTCCCCTATCAAAAAGAAAACGCCGAAGAGAAAAGAGAAAAATTAGTGCGTTAATCAGCCTTTTTTTTTTTTTTTAAAACGGGGGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGc‑c                                                                                                                                                                                     <  2:468798/237‑1 (MQ=255)                                                 aGTAGAAAAACAAAAAAGAAT‑ATAGTTAAGACTGGTGTTATAGGCGGGGGAATAAATTTCGCGGGTGTTATACATTTAATCCTTTATGGCCCTCCAACCAAAAAGAAAAAGCGGATGAGCTAAGAAAAAAATTAGTTCGATAGCCAGCGTTTATATTTTTTGTTTAACCGGGGGG‑TTG‑AA‑TTGTTT‑TCTC‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGtt                                                                                                                                                                               <  2:972473/238‑1 (MQ=255)                                                        aaTAAAAAAAAAAG‑ATATTTCAGCCTGGATGTATCCGCGGGGGAAAAAAATTTACGGCGGTTATCCCATTGTTCTTTTATTGCTCACCTACCAAAAAGAAGAAGCCAACGAGAAAAGAAAAAAATATGTTCGTTAATCACCCTTTATATTTATTGTTTAACCTGGGGG‑TTA‑AA‑TTTTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTatcatc                                                                                                                                                                       <  1:443884/239‑1 (MQ=255)                                                                        tacctcatgcTGGAGTTACACGCGCTGGAATAAAATTTACGGGCTTTACACTTTGGACCCTTTATGGCACACCTAGCAAAAAAAAGGAGGCGACAGGTCAAGAAAAAAAAGTCTGCGCTACACACCCGTTTTATTTATTGTTTAGCCTGGGGG‑TAA‑AT‑TTGTT‑‑TCCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGtt                                                                                                                                                       <  2:115863/230‑1 (MQ=255)                                                                                                                    gTTATACCATTGATCCTTTATTGGTCCCCTACCAAAAAGAAAAAGCCGAGGAGCTAAGAGAAAAATTAGTCCGCTAACCAGCCGTTATATTTATTGTTTAAACGGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTatcatcat                                                                                                                                                                     <  1:211309/182‑1 (MQ=255)                                                                                                                           ctttGATCTTTTATTGGCCCCCTACCAAAAAGAAAAAGCCGAAGAGCTAAGAGAAAAATTAGTTCGTTAATCAGCTTTTATTTTTTTTTTTAAAACGGGGGT‑TTA‑AT‑TTTTTT‑TCTC‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                    <  1:544188/237‑1 (MQ=255)                                                                                                                              tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑TA‑TTTTTT‑TTTT‑CCA‑CCGCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGC‑CAACGTTTTATCCTCCT‑CCGTACCCTc                                                                                                                                                          >  2:211309/1‑182 (MQ=255)                                                                                                                               gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CCA‑CCGCCATGAGGAAATCAATCTGCCCCCAGCGCCAGTGC‑CAACCGTTTATCATTAT‑CCGTTAGCCCGTGTAACGCCGGGCTGGACCAAAACTGCTTTTACAATTTCGGTTATAAATCGAgttgt                                                                                                >  1:430787/1‑239 (MQ=255)                                                                                                                                     ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGC‑CAACGGTTTTTCATCAT‑CCGTAAGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAAACGAGTTGTTCCTGc                                                                                          >  1:567766/1‑239 (MQ=255)                                                                                                                                      tATTGCTCCCCTACCAAAAAAAAAAAGCCGACGAGCTAAGAGCAAAATTATTCCGTAAATCAGCTGTTATATTTATTTTTAAAACTGGGGG‑TTA‑AA‑TTGTTG‑TCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg                                                                                         <  1:914921/239‑1 (MQ=255)                                                                                                                                           gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTTTTG‑TCTT‑CAA‑CCCCCATGAGAAACTCACTCTGCCCCCAACGCCCGTGC‑CCACGGTTTTTCATCAT‑CCGTACGCTCGTTTACCGCCCGGCTGGACAAAAACATCTTTAACAATTTAGGGAATAAATCGAGTTGTTCCTGCGTtgct                                                                                    >  2:268980/1‑239 (MQ=255)                                                                                                                                            tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑TA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGC‑CCACGGTTTATCATCAT‑CCGTACACTCGTTTTCCGCCCGGCCGGACAAAAACTGCTTTAACCAATCCGGGAATAAATCGAGTTGTTTCttggtttct                                                                                    >  2:977994/1‑230 (MQ=255)                                                                                                                                            tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTTCTGCGTtgct                                                                                    >  1:897092/1‑238 (MQ=255)                                                                                                                                             cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTT‑GA‑TTGTTG‑TGCT‑CCA‑CCGCCCTGGGAAAATTACTTCCCCCCCAACCCCCGTGCGAAACCTTTTTTCA‑CAT‑CCGGACGCCCGTTTTCCACCCGGCTCGACCCAAACTGCTTTTACCATTTAGGTaaaaaaattctgtgtttcttcgtcgctcg                                                                                  >  2:1147034/1‑216 (MQ=255)                                                                                                                                              aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCCCGTTTACCCCCCGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAACGAGTTTTTTCTGCggttcttc                                                                                  >  1:1091478/1‑231 (MQ=255)                                                                                                                                                                           aaGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TGTT‑CAA‑CCCCCATTAGAAAATCACTCTGCCCCCAGCGCCAGTGC‑CAACGGTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGGTGGGCAAAAACTGCTTTCACAATTTCGGCAATAAATCGAGTTTTTCCTGCGGTTCCGCCGCCAGTCCCATGGAAAACGGGGGaaagca                                                    >  2:749916/1‑237 (MQ=255)                                                                                                                                                                                 aaaaTTTGTTCCATAAACAGCCCTTATTATTTTTTTTTAAACCGGGGG‑TTT‑AATTTTTTG‑TTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGca                                               <  1:295498/239‑1 (MQ=255)                                                                                                                                                                                 aaaaTTGGTTCCTTAATAACCCTTTATTTTTTTTGTTAAACCTGG‑GG‑TTT‑AA‑ATTTTT‑TCCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                              <  1:500990/239‑1 (MQ=255)                                                                                                                                                                                   aattgtttcgttaaaccccctttATTTTTTTTG‑TTAAACTGGGGG‑TTT‑AA‑ATTTTGTTCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACAt                                            <  1:1030252/218‑1 (MQ=255)                                                                                                                                                                                      tAGTTCGTTATACACCCTTTTTTTTTTTTATTAAACCTGG‑GG‑TTT‑AA‑TTTTTT‑TTTTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                         <  1:99038/239‑1 (MQ=255)                                                                                                                                                                                        tgtccatAATCAGCCGTTATTTTTATTGTTAAAACTGGGGG‑TTG‑AA‑TTTTTG‑TCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagga                                       <  1:307402/233‑1 (MQ=255)                                                                                                                                                                                             cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑ATG‑TA‑TTGTTT‑TTTT‑TCA‑ACGCCATGAGAAAATAACTCTGCCCACAACCCCCATGC‑CCACGTTTTTTTATCATCCCGTAC‑CTCTGTTACCGCCGGGGTGGACAAAAAATTCTTTTACAATTCATGGAATAAATAGCGGTGTTTCTGCGGTTCTTCCGCCAGTCCATTGTAAAACGGGGAAAAAAAAGGCACATGAGGGGAAtt                                  >  2:1231099/1‑239 (MQ=255)                                                                                                                                                                                                ccagctcttaaaatttgttTTATAAAGTGGCGT‑TTG‑AT‑TTTTTG‑TCTA‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                               <  1:757242/227‑1 (MQ=255)                                                                                                                                                                                                acaaGCCTTTTTATTTATTGTTAAACCGGGCGG‑TTG‑AT‑TTTTTT‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                               <  1:872061/236‑1 (MQ=255)                                                                                                                                                                                                     ccGTTATATTTATTGTTTAAACTGGCGG‑TTG‑GA‑TTGATG‑TCTT‑CCG‑ACGCCATCAGAAAATCACTCTTCCCCCAACGCCAGGGC‑CAACCTTTTATCATCAT‑CCGAAAGCTCGTTTTTCCCCGGGCTGCACCAAAAATGCTTTAACAATTCCAGCAATAATTCATGTTGTTCCGGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGATAAACAAATGCACATGAGGAGAAGTATCCAGCa                          >  2:636701/1‑239 (MQ=255)                                                                                                                                                                                                                attGTTTAAACTGGCGG‑TTG‑AA‑TTGTTT‑TCTT‑CAA‑CCGCCTTGAGAAAATCACTCTGACCACAGCGCCAGTGC‑CAACGTGTTTTCATCAT‑CCGTTCGCTCGTTTAACGCCGCGCTGGACAAAAAATTCTTTTACAATTCAGGTAATAAATTGAGTTGTTTCTGCGTTGCTGCCGCCAATCCATTGGAAAAAGGGGAGAAAAAAGGGACAATAGGAGAATTATCCAGGATATTTAACGcc               >  1:1331392/1‑239 (MQ=255)                                                                                                                                                                                                                 attattAAACCGGCGGTTTG‑AC‑TGTTTG‑TCAC‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc               <  1:1231099/235‑1 (MQ=255)                                                                                                                                                                                                                  tGTTTAAACTGGCGG‑TTT‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGGTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg             >  1:753409/1‑239 (MQ=255)                                                                                                                                                                                                                  tGTTTAAACTGGCGG‑TTT‑AA‑TTGTTG‑TCTT‑CAA‑ACCCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCGGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTCCCTTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg             >  1:760198/1‑239 (MQ=255)                                                                                                                                                                                                                  tGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TTTT‑CAA‑CCGCCATGAGAAAATCACTCTGCCCCCAACGCCAGTGC‑CCACGTTTTATCATCAT‑CCGTACGCCCGTTTACCGCCGGGCTGGACAAAAAATGCTTTTACAATTCAGGTAATAAACTGAGTTGTTCCTGCGGTGCTGCCGCCCGTCCATTTGAAAACGGGGGAAAGAAAAGCACAAGAGGAGGATTATCCAGCCTATTTAACGcccg             >  1:66511/1‑237 (MQ=255)                                                                                                                                                                                                                  tGTTTAAACTGGCGG‑CTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACCCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCCCGTTTTCCGCCGGGCTGGACAAAAACTTCTTTAACAATTCCGGTAATAAATCGAGTTGTTCCTGCGGTTCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTTACGCCaa             >  1:884981/1‑238 (MQ=255)                                                                                                                                                                                                                  tGTTAAAACGGCCGT‑TTA‑AT‑TTTTTG‑CCTA‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg             <  1:1095048/239‑1 (MQ=255)                                                                                                                                                                                                                   attttttaacggggcggtaaatgtgttc‑tCTC‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc               <  2:24955/216‑1 (MQ=255)                                                                                                                                                                                                                    tttAACCGGGGGG‑TTA‑AA‑TTGTTG‑TCTT‑AAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                    <  1:1315240/150‑1 (MQ=255)                                                                                                                                                                                                                              ggg‑TTGAAA‑TTGTTT‑TTTC‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa   <  1:1012115/237‑1 (MQ=255)                                                                                                                                                                                                                              cGG‑TTG‑AA‑TTTTTT‑TCTT‑TAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGGTTTATTATCAT‑CCGTACGCCCGTTTTCCCCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGGAATAAATCGAGTTGTTTCTGc                                                                                          >  2:1315240/1‑150 (MQ=255)                                                                                                                                                                                                                                gcgtttaattttttg‑tCTT‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa   <  1:176128/226‑1 (MQ=255)                                                                                                                                                                                                                                 ggtttaattttttg‑tCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc  <  1:268980/227‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      TAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAG‑ATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTG‑AA‑TTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC‑CAACGTTTTATCATCAT‑CCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAC  >  NC_000913/2151456‑2151913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑